Shimadzu CBM-40 CL User manual

228-97201A
Jun. 2022
System Controller
for Shimadzu Liquid Chromatograph
CBM-40 CL
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.

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Read this Instruction Manual thoroughly before using
the product.
Thank you for purchasing this product.
This manual describes accessories, options and usage of this product.
Read this manual thoroughly before using the product and operate the product in
accordance with the instructions in this manual.
The following instruction manuals are included with the product in booklet or PDF
document. The PDF document is contained in the instruction manual CD-ROM (part
number 228-97193-41).
Document Name Document
Number Description
Instruction Manual (PDF) 228-97201 This book.
System Guide
(PDF) 228-97194
This section describes the entire system.
Optimization of system performance, analysis
procedures, troubleshooting methods,
validation, installation, etc. are described.
Safety Guideline
(Booklet/PDF) 228-97195 Precautions for safe use are listed.
Be sure to read "Safety Instructions" before using this product. "Safety Instructions"
includes precautions, product warranties, and after-sales service to ensure safe use.
The instructions for use must be kept with this product so that they can be viewed at
any time.
IMPORTANT
• If the user or usage location changes, ensure that this manual is always kept
together with the product.
•If this manual or a product warning label is lost or damaged, immediately contact
your Shimadzu representative to request a replacement.
•To ensure safe operation, read all Safety Instructions before using the product.
•To ensure safe operation, contact your Shimadzu representative if product
installation, adjustment, re-installation (after the product is moved), or repair is
required.
ã 2020-2022 Shimadzu Corporation. All rights reserved.
Original version is approved in English.
Introduction
CBM-40 CL i

Notice
• Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
• All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission from Shimadzu Corporation.
•Illustrations in the instruction manual may differ from the products you use.
• Nexera and LabSolutions are trademarks of Shimadzu Corporation.
Windows is a registered trademark of Microsoft Corporation in the United States and/or
other countries. iPhone and iPad are registered trademarks of Apple Inc. Other company
names, product names, service marks and logos that are listed in the instruction manual
are trademarks and registered trademarks of each company. TM and ® marks are not
specified in the text.
Indications Used in This Manual
The following precautions are included in this manual depending on the magnitude of
the hazard or damage:
Indication Meaning
Indicates a potentially hazardous situation which, if not
avoided, could result in serious injury or possibly death.
Indicates a potentially hazardous situation which, if not
avoided, may result in minor to moderate injury or equipment
damage.
Emphasizes additional information that is provided to ensure
the proper use of this product.
The following symbols are used in this manual:
Indication Meaning
Indicates an action that must not be performed.
Indicates an action that must be performed.
Indicates information provided to improve product performance.
Indicates the location of related reference information.
ii CBM-40 CL
Introduction

Electromagnetic Compatibility
Descriptions in this section apply only to the following models:
•228-65502-55 CBM-40 CL
This product complies with European standard EN61326, class B for electromagnetic
interference (Emissions) and industrial electromagnetic environment (Immunity).
n EN55011 Emissions (Electromagnetic Interference）
This is a class B product. When this product causes an electromagnetic disturbance to
devices being used near this product, create an appropriate distance between those
devices and this product in order to eliminate the disturbance.
Operating Nexera CL System
nIntended Use
This system is designed to perform qualitative and quantitative analysis of target
compounds in a sample matrix and can be used as a system controller of the liquid
chromatograph for general in vitro diagnostic applications. However, only personnel who
have received appropriate training on use of the system can use it for these purposes.
nCalibration
A calibration curve should be generated for each analyte in appropriate methods. At
least four out of six non-zero standards should meet the appropriate criteria, including
the calibration standard lower than the assumed LOQ and the calibration standard at
the highest concentration.
nQuality Control
Implement quality control of the instrument by routinely measuring at least one each of
four types of quality-control samples (a sample with a normal concentration level, a
sample with a concentration level higher than normal as well as a sample lower than
normal, and a blank sample). Check the selectivity/specificity in the matrix used for the
actual sample, the accuracy, correctness, recovery rate, dynamic range, linearity, and lower
limit of quantitation.
Based on these assessment results, check that the results are within the permissible range.
If the assessment results are out of range, the measured data may be invalid, so do not
use analysis results obtained from the instrument in question until it can be confirmed
that it is functioning normally. When, for example, analyzing samples that contain
complex sample matrices such as serum, plasma or urine, it may be possible to obtain
stabilized data by using an appropriate pre-treatment or an internal standard.
CBM-40 CL iii
Electromagnetic Compatibility

Contents
Introduction Indications Used in This Manual ......................... ii
Electromagnetic Compatibility ............................ iii
Operating Nexera CL System .............................. iii
1Overview 1.1 Features ............................................................. 1
1.2 Component Parts ................................................. 2
1.3 Optional Parts ..................................................... 3
2Parts Identification
and Function
2.1 Front .................................................................. 4
2.2 Back ................................................................... 5
2.3 Bottom ............................................................... 7
3Web Screen 3.1 Connect to the Web Screen (Windows) ................. 8
3.1.1 PC Specification ................................................. 8
3.1.2 Browser Settings ................................................ 8
3.1.3 Display Web Screen ........................................... 10
3.2 Connect to the Web Screen (iPad) ...................... 10
3.2.1 iPad Specification .............................................. 11
3.2.2 Preparation .................................................... 11
3.2.3 Display Web Screen ........................................... 11
3.2.4 Notes on Operating the iPad ................................. 11
3.3 Group Monitor Screen ....................................... 14
3.3.1 HPLC Explorer Area ........................................... 15
3.3.2 Status Summary Area ......................................... 17
3.3.3 Simple Device Monitoring Area ............................... 20
3.4 Device Monitor Screen ....................................... 21
3.4.1 Executing Analysis Status Screen ............................. 22
3.4.2 [Device Configuration] Screen ................................. 30
3.5 Group Management Screen ................................ 32
3.5.1 Change the System Name .................................... 36
3.5.2 Move the System to Another Group ......................... 38
iv CBM-40 CL

3.5.3 Create a New Group ......................................... 39
3.6 Utility Screen .................................................... 41
3.6.1 Store System Parameters Collectively on the PC ............ 42
3.6.2 Load System Parameters That Were Saved at Once ......... 44
3.6.3 Initialize Device Parameters ................................... 47
4Applied Operation 4.1 External Input/Output Terminal .......................... 48
4.1.1 Event Terminal Signals ........................................ 48
4.1.2 Connect the Event Cable ..................................... 49
4.2 Network Settings ............................................... 50
4.2.1 When Setting From Connected Device ....................... 50
4.2.2 When Setting From the Web Screen ........................ 51
4.3 GxP Enhancement Function ................................ 53
4.3.1 Set Up GxP Enhancement Function .......................... 53
5Maintenance 5.1 Periodic Inspection ............................................. 54
5.2 Cleaning the Exterior ......................................... 55
5.2.1 Usual cleaning ................................................. 55
5.2.2 Decontamination cleaning ................................... 55
6Technical
Information
6.1 Environment Setting List .................................... 56
6.2 Error Type ........................................................ 57
6.3 Specifications .................................................... 58
Index ........................................................... 60
CBM-40 CL v
Contents

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This instrument is a system controller for a Shimadzu liquid chromatograph. The system
controller builds a system by connecting the solvent delivery unit, the autosampler, the
column oven, the detector, etc., and performs analysis operations by controlling gradients,
sample injection, data collection, etc. for each unit according to the analysis conditions
of LabSolutions (chromatography data system). And it can monitor data for liquid
chromatograph systems with various equipment configurations. This instrument basically
controls the Nexera CL series.
1.1 Features
nNetwork Functions
Correspond with Ethernet Connection.
The instrument has the network function normally. By connecting with Shimadzu liquid
chromatograph data processing software (workstation), it can set up conditions, execute,
monitor, and process analysis from the PC.
Equipment Monitoring Function by Smart Device
The operation status of the equipment, analysis progress situation, maintenance
information, etc. can be monitored from a smart device such as an iPad via the network
even where you are away from the system.
nNumber of controllable modules
8 units. When optical connenctor expansion option is used: 12 units.
1Overview
CBM-40 CL 1
1

1.2 Component Parts
This equipment consists of the following parts: Check the contents and quantity when
unpacking.
No. Part Name Part No. Q'ty Remark
－CBM-40 CL Main body －1
－Safety Guideline (Booklet) 228-97195*1 1
－
Instruction Manual/
Nexera CL Series System
Guide
(CD-ROM)
228-97193-41 1
1 LAN Cable 228-61083-41 1 2 m
2Waste Liquid Tube
Silicone 228-25162-03 1
3 Drain Adapter 228-42204 1
4 Drain OUT, STD 228-42205 1
5 Drain OUT, CTO 228-42206 1
6 L Type Joint 035-61561-12 1
7 Straight Joint 228-28163 1
* 1 It contains cautions for use regarding the instrument.
2CBM-40 CL
1 Overview

1.3 Optional Parts
The optional parts that can be added to this instrument are shown below.
For details of optional parts, please contact our sales office/distributor or our designated
service representative.
Option Name Part No. Features
Optical Cable 0.6 m 228-77025-06
It is an optical cable for communication
between the system controller and each
module.
Optical Cable 0.8 m 228-77025-08
Optical Cable 1 m 228-77025-10
Optical Cable 2 m 070-92025-52
Optical Cable 3 m 070-92025-53
Optical Cable 5 m 070-92025-54
Remote Cable 228-28253-91
It is used for input or output of error / start /
stop signals from other equipment.
"4.1 External Input/Output Terminal"
P.48
Optical Connector
Expansion Board 228-70481-41
Optical connectors can be added with this
option. It can be mounted on CBM-40 CL and
expands from standard 8ch to 12ch.
CBM-40 CL 3
1.3 Optional Parts
1

2.1 Front
There is no power button on the CBM-40 CL. Of the connected pumps, turn
on/off the system power with the power button of the pump that has the lowest
address number.
No. Name Description
1 [CONNECT] Lamp It lights up when connected to the workstation.
2 [ERROR] Lamp It lights up when an error occurs in the system.
3 Side Cover Piping such as suction tubes can be passed through it. This
cover is removable.
2
Parts Identification and Function
4CBM-40 CL

2.2 Back
CBM-40 CL 5
2.2 Back
2

No. Name Description
1 [REMOTE] Connector
It is used for connection with each module.
Connect the autosampler to Ch.1.
2 [INIT] Switch
This is an initialization button to perform a factory
reset.
• All settings including device log,
network setting, etc. are initialized to
the factory default setting.
• While holding down the [INIT] switch,
turn the main power switch to "ON"
and initialize it by holding the [INIT]
switch for 20 seconds.
3 [ETHERNET] Connector It is a terminal for connection with a PC.
4External Input/Output
Terminal
It is a terminal for connection with external
equipment.
"4.1 External Input/Output Terminal" P.48
5 [OPTION] Connector Not used.
6 [AC REMOTE] Connector Not used in CL model.
7 [AC IN] Connector It is a terminal for connecting the power cord.
8 Main Power Switch Switch ON/OFF the main power supply of the
equipment.
6CBM-40 CL
2 Parts Identification and Function

2.3 Bottom
No. Name Description
1 Drain Port
When a leakage occurs in the unit located on top of the
CBM-40 CL, the liquid is discharged from this drain port.
Please connect the drain adapter according to the system
guide.
CBM-40 CL 7
2.3 Bottom
2

CBM-40 CL can be connected to the Web screen from the Web browser of the PC
connected to the network. Web screen provides analysis monitoring, system controller
environment settings etc.
• On the Web screen, only two ovens are displayed (Oven C, D can not be
displayed).
3.1 Connect to the Web Screen (Windows)
This section explains the case of Windows.
3.1.1 PC Specification
Describes the specifications of the PC necessary for accessing the Web screen.
When building a network, please consult with the department to be used, the
network management department of the business office, or the person in charge.
Item Description
CPU Intel Pentium E5300 (2.6 GHz) or more
Memory Recommended 2 GB or more
LAN Adapter 100 Base-TX compatible
Display 1024´768 or more
OS Windows XP / 7 / 8 / 10
Browser Internet Explorer 6 to 9.
Internet Explorer 10 and 11 are compatible view only
Language English
3.1.2 Browser Settings
In order to access the web screen of the system controller, several settings are required
in the web browser.
The following shows how to set Internet Explorer 11 as an example.
3Web Screen
8CBM-40 CL

1Click [Compatible View Settings] from the [Tools] menu to register the URL of
the system controller.
• For Internet Explorer 10 and 11, compatible view settings must be made.
• For Internet Explorer 6 to 9, compatible view setting is unnecessary, so skip this step.
2Click [Internet Options] from the [Tools] menu of the browser.
The [Internet Options] screen appears.
3Add the URL of this device to the zone of the local intranet.
1On the Security tab, select Local intranet.
2Click [Sites].
3Click [Advanced] Settings.
4In [Add this website to the zone], enter the URL of this device. (http://[IP address
of this device])
5Click [Add] and confirm that the URL of this device is displayed on [Web sites].
4Set the security level.
1On the Security tab, click Custom Level.
2Set the items in [Setting] as follows
Item Configuration
[ActiveX controls and plug-ins] - [Run ActiveX controls and
plug-ins] Enable
CBM-40 CL 9
3.1 Connect to the Web Screen (Windows)
3

Item Configuration
[ActiveX controls and plug-ins] - [Script ActiveX controls marked
safe for scripting] Enable
[Script] - [Active Scripting] Enable
[Download] - [File Download]*1 Enable
[Miscellaneous] - [Access data sources across domains] Enable
*1 Set it to save method file and sequence file on PC.
• If software with pop-up blocking function (security software or
browser toolbar) is installed on the PC, the Web screen may not be
opened. Please disable popup blocking function for system
controller URL. In the case of Internet Explorer, delete the
temporary file after disabling the pop-up blocking function.
• When the history data of Internet Explorer increases, the updating
of the Web screen slows down and the operation becomes unstable.
Click [Settings] in [Browsing History], and set [Disk space to use] to
'100' MB.
• If [Use a proxy server for your LAN] is checked on the [LAN settings]
on the [Connections] tab, please check [Bypass proxy server for local
address], or click [Advanced] settings and add the URL of this device
to [Exceptions].
• Avoid continuous operation by opening three or more web screens.
• Do not press shortcut keys such as "F5" (update) or "F11" (full
screen display). An error may occur or display on the screen may
be lost.
• When browser script errors occur, the screen update stops. By checking
[Browsing] - [Display a notifications about every script error] in Settings on
the Advanced tab, cause of the error can be easily identified when an error
occurs.
• To print the display contents of the screen such as maintenance information
and device configuration, press the "Ctrl" + "P" key.
•When printing the contents of the Web screen, check [Page Setup] - [Print
Background and Color Image] in the [File] menu.
3.1.3 Display Web Screen
Enter the following URL in the browser.
http://(IP address of this device)/html/
3.2 Connect to the Web Screen (iPad)
This section explains operation with an iPad.
10 CBM-40 CL
3 Web Screen

3.2.1 iPad Specification
Specifications that checked the operation are shown below.
Item Description
Model Wi-Fi model
OS iOS8～11
Browser Safari
Language English
3.2.2 Preparation
Add a wireless LAN adapter (Wi-Fi) to the same network as this device, and make the
iPad ready for connection.
• When building a network, please consult with the department to be used, the
network management department of the business office, or the person in
charge.
3.2.3 Display Web Screen
Enter the URL of this device in the browser.
http://(IP address of this device)/html/
By registering the Web screen on the home screen, the Web screen can be displayed just
by tapping the registered icon. For details, refer to the instruction manual attached to iPad.
3.2.4 Notes on Operating the iPad
Describes notes on displaying web screen from iPad.
nAbout Automatic Lock Function
The web application's operation may become unstable when locked due to the automatic
lock function of the iPad. In that case, turn off the automatic lock function by the
following procedure.
1Click the setting icon from the iPad's home screen.
Select "General" on the setting screen.
2Select "Auto lock" in General setting.
In the automatic lock setting screen, select "OFF".
CBM-40 CL 11
3.2 Connect to the Web Screen (iPad)
3

nAbout Continuous Operation
If connecting to the Web screen for a long time from the iPad, the operation of the
iPad may become unstable. When using continuously, please note the following points.
• Please close all screens of Safari about once a day.
• Avoid continuous operation by opening three or more operation screens.
Normally open the "Group Monitor" screen and "Device Monitor" screen one
by one and use them.
•If the operation of the iPad becomes unstable, turn off/on the iPad.
nAbout Safari's Cache
If the operation and display of the web screen are incorrect due to Safari's cache, please
delete the cache in the following procedure.
1Click the setting icon from the iPad's home screen.
On the setting screen, select "Safari".
2Select "Clear cache" in Safari setting.
A confirmation dialog will be displayed, so select "delete".
nUpdate Screen Button
Please do not press the update button in the address bar. An error may occur or screen
design may be lost.
12 CBM-40 CL
3 Web Screen