SOPHION BIOSCIENCE QPatch Compact User manual

QPatch Compact Manifold Recovery Guide
In case of unforeseen mechanical events, the manifold may
be jammed into a position where it will not release itself
during normal operation. Follow this guide to recover the
manifold via the built-in recovery function.
Start up and attempt homing
1. Read through guide
Please read the entire guide before starting the recovery process. Some steps
need to be monitored manually.
2. Close latches
Open the instrument lid and ensure that
both latches are fully engaged.
If the manifold has been dislodged from the
interface module so latches cannot fully
close, make sure to push the manifold and
the interface module together as much as
possible without closing the latches.
Caution! Never use excessive force to
attempt closing the latches.
3. Start up the QPC
Turn on the instrument (on the left-hand side). Log in via the touchscreen using
your normal login credentials for the QPC database.
4. Wait for homing
If the manifold releases itself during homing, remove the QPlate after homing is
done and proceed to checking manifold latches and verifying functionality.
If the manifold is stuck, the instrument will make a low-pitched humming during
homing and the “Preflight Check” status will stay in “Initializing QPlate slot”. In
that event proceed to the next step.

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Run recovery function
1. Enter Maintenance menu
Click the Maintenance icon on the left-hand side to enter the maintenance
menu.
2. Start recovery function
Press the “Recover from jam” button in the “Manifold” menu to start the
recovery process. A countdown timer next to the button will show the remaining
time for the process.
The recovery function will attempt moving the manifold back and forth between
the intra- and extracellular positions via a forced move. The instrument may
make a low-pitched humming during the process. This is normal.
The humming should stop during the process or at the latest a few seconds
after the countdown has ended.
Caution! If the humming does not stop shortly after the countdown, open the
instrument lid to stop the motor.
3. Monitor manifold move
Monitor the manifold through the pipetting access cut-out during the process.
When the manifold starts lifting, it is recommended to very gently pushing down
on the protruding QPlate handle a couple of times to aid the release process.
Caution! Do NOT attempt to remove the QPlate during the recovery process.
Caution! Do NOT touch the manifold during the recovery process.
4. Wait for countdown
When the countdown has stopped, remove the QPlate if possible.
5. Close latches
Open the instrument lid to check and close the manifold latches. See the user
manual and installation manual on how to check the manifold latches.

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Verify mechanical functionality and close software
1. Insert QPlate
Insert a QPlate. Ensure that the plate is fully inserted since the next steps are not
dependent on the plate sensor.
2. Move to home
Click the “Move to home position” button and verify that the manifold moves to
the home position.
3. Move to intracellular
Click the “Move to intracellular position” button and verify that the manifold
moves to the intracellular priming position.
4. Move to extracellular
Click the “Move to extracellular position” button and verify that the manifold
moves to the extracellular priming position.
5. Move to home
Click the “Move to home position” button and verify that the manifold moves to
the home position.
6. Remove QPlate
Remove the QPlate.
7. Close software
Click the “Logout” menu button and click “Log out”
Click the “X” in the upper right corner to close the software.
The software can now be restarted, and the instrument used for normal
operation.
Caution! It is important to close the software to power off the motor after the
recovery process.
Software button explanations
Click the button to enter preflight mode.
In this window you can initiate and setup your experiments.
Not applicable in first release
Click the button to open Maintenance mode.
In this window you can perform all maintenance tasks.
Click the button to view all completed results.
Click the button to get system information and logout possibility.