
Positioning of the CO2 Controller is crucial in the aspect of vibration isolation. There
are two rude vibration sources implemented in the CO2 Controller e uipment. They
are a mixing fan and a gas transport pump. Please choose such a place for the CO2
Controller e uipment, which provides efficient separation of it from the vibration
isolation table holding the microscope.
Steps of Installation
Set the pressure regulator on the CO2 tank to a value between 1 and 2 Bar. Connect
the outlet port of the regulator to the inlet port of the CO2 Controller device (using PU
8/6 tubing).
Fill the humidifier with water to the desired level, approximately 2/3 of the bottle.
Connect the “OUT” port of the CO2 Controller to the inlet port of the humidifier.
Please keep the outlet and inlet ports of the humidifier bottle in mind.
Connect the outlet port of the humidifier to the inlet port of the incubator (marked
with an arrow).
Connect the outlet port of the incubator to the “IN” port of the CO2 Controller device.
Open the output valve on the pressure regulator of the CO2 tank.
Plug in the device and turn it on with the MAINS switch. The internal fan starts up
and the screen turns on. The regulator is ready after about 5 seconds. The CO2
concentration is set to 5% by default. The device measures CO2 concentration
starting at 2.5%, the display says 0% until the threshold is reached. The
concentration rises up to 6% after the first CO2 addition cycle. As the gases continue
mixing the concentration drops, and as it gets below the set value, the device adjusts
the dosing. Then the concentration stays between constant values.
To adjust the concentration push buttons F and ▲ or ▼ at the same time. Release F
to save the current setting. This will be the new default the next time the device is
switched on.
After the second cycle, the gas may be circulated through the incubator pushing the
button PUMP. When setting a lower value than used previously, the CO2
concentration drops faster if the incubator is opened.