tecan MIO2 User manual

MIO2 Operating Manual, 394934, en, V3.0
Operating Manual
Monitored Incubator Option 2 (MIO™)
6 Positions
4 Positions

© 2017, Tecan Trading AG, Switzerland, all rights reserved.
Information contained in this document is subject to change without notice.
Document Status Sheet
Title: MIO2 Operating Manual Part number: 30032210.04
ID: 394934, en, Version 3.0 Translated from: -
Version Revision Issue Document History
1 0 2007-12-05 New edition
1 1 2009-11-09 Minor corrections
2 0 2010-03-12 Added the 4 slotted monitored incubator
2 1 2014-08-07 Added the installation of the instrument plate and the connection of the CAN
cable
3 0 2017-08-31 Corrected shutters’ description in chapter “Part Locations”
Added chapter “Customer Support”
MIO2 is available for Freedom EVO and Fluent
“Product Safety Signs” added

MIO2 Operating Manual, 394934, en, V3.0 i
Table of Contents
Table of Contents
1 About This Manual
1.1 Scope of This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Manufacturer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.3 Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.4 Improper Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.5 Warranty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.6 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.7 Reference Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.7.1 Instrument Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.7.2 Software Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.7.3 Other Reference Documents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.8 Compliance with Laws and Standards. . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.9 Document Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.10 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
2Safety
2.1 Safety Message Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.1.1 Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.1.2 Safety Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 General Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.3 Operating Company. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.4 User Qualification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.4.1 Operator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2.4.2 Key Operator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2.5 Product Safety Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.6 Decontamination Declaration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
3 Technical Data
3.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Product Identification and Appendix Labels. . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.3 Dimensions and Weights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.4 Operating Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.5 Electrical Module Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.6 Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.7 Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3.8 Emissions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
4 Description of Function
4.1 Principles of Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.1 Part Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

ii MIO2 Operating Manual, 394934, en, V3.0
Table of Contents
5 Installation
5.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.1 Labels on the Packaging. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5.2 Incubator Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
5.2.1 Connecting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
5.2.2 Multiple Incubators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.2.3 Leveling Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
6 Operation
6.1 Operating Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.2 Display and Operating Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.3 Operating the MIO2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
6.3.1 Safety Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
6.3.2 Process Validation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
6.3.3 Switching the Incubator On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
6.3.4 Switching Incubator Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
6.3.5 After an Aborted Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
6.3.6 Incubating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
7 Maintenance
7.1 Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.2 Cleaning Agents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.3 General. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
7.4 Special Maintenance Tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
7.4.1 Incubator Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
7.4.2 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
8 Troubleshooting
8.1 Troubleshooting Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2
9 Disposal
10 Customer Support
10.1 Contacts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1
11 Index

1 - About This Manual
Scope of This Manual
MIO2 Operating Manual, 394934, en, V3.0 1 - 1
1 About This Manual
This Operating Manual describes the MIO2 and provides all the information
required for operating it safely and for maintaining it in good working order. This
manual must be read carefully before performing any work on the MIO2 and
before using it.
This chapter outlines the purpose of this manual and specifies the product
referred to. Furthermore, it explains the use of symbols and conventions as well
as further general information.
This Operating Manual contains no software description. For more information on
the software please consult the corresponding software manual. Refer to section
1.7 “Reference Documents”, 1-2.
1.1 Scope of This Manual
This manual is applicable for MIO2 (6 positions and 4 positions) valid from the
serial number 706005497 designated for operation on instruments (e.g., Freedom
EVO and Fluent).
Note: The MIO2 with 4 position is not compatible with the Freedom EVO 75.
1.2 Manufacturer
1.3 Intended Use
The MIO2 is used on Tecan instruments to incubate and / or shake substances in
microplates.
The MIO2 is an instrument component. The intended use is defined for the
instrument where it is used on. Refer to the corresponding Operating Manual of
the instrument. Refer to section “1.7 “Reference Documents”, 1-2.
Tecan Schweiz AG
Seestrasse 103
CH-8708 Männedorf
Switzerland

1 - 2 MIO2 Operating Manual, 394934, en, V3.0
1 - About This Manual
Improper Use
1.4 Improper Use
Improper use may prejudice the MIO2 safety concept.
The MIO2 must not be used with options or components that are not approved
by Tecan.
The MIO2 is not explosion-proof and should not be installed in locations
where there is a hazard of explosion.
The MIO2 should not be used in the absence of functional safety devices.
The MIO2 is not suitable to defrost frozen microplates.
Only liquids suitable for the MIO2, with respect to the chemical resistance of the
liquid system and explosion/fire hazard, may be used. Special applications
requiring the use of hazardous substances are run at the user’s own risk.
1.5 Warranty
The MIO2 must not be used with components that are not approved by Tecan.
The use of unapproved components may impair the safety concept of the MIO2.
This would invalidate any warranty of safety and compliance to national and
international standards, as required for UL/CSA certification, by EC directives, etc.
1.6 Trademarks
The product names, whether registered or unregistered trademarks, mentioned in
this manual are reproduced solely for identification purposes and remain the
exclusive property of their respective owners. For simplicity reasons, the
trademark symbols such as ®and ™ are not repeated in the manual.
1.7 Reference Documents
This section provides a list of the documents that are needed or may be useful
when using the MIO2.
The Doc IDs listed below are root numbers. Therefore, they do not contain
information about the language, document version, or the medium (data storage
medium, hard copy, downloadable file, etc.) of the document.
Note: On the basis of your order configuration, the Operating Manuals for optional
equipment apply as well.
Check the scope of the corresponding document to ensure that you are in
possession of the correct version.
The Doc ID does not refer to ordering information. When placing orders, please
refer to the number on the binder, CD casing, etc.

1 - About This Manual
Compliance with Laws and Standards
MIO2 Operating Manual, 394934, en, V3.0 1 - 3
1.7.1 Instrument Manuals
Freedom EVO®Operating Manual (Doc. ID 392886)
Fluent®Operating Manual (Doc. ID 396327)
Fluent®Operating Manual (Doc. ID 399706)
Fluent®Reference Manual (Doc. ID 398090)
Fluent®Reference Manual (Doc. ID 399937)
1.7.2 Software Manuals
Freedom EVOware®Software Manual (Doc. ID 393172)
Freedom EVOlution™ Application Software Manual (Doc. ID 394802)
FluentControl™ Application Software Manual (Doc. ID 396329)
Instrument Software Manual (Doc. ID 392888)
Fluent®Instrument Software Manual (Doc. ID 396330)
1.7.3 Other Reference Documents
Certificate of Decontamination Form (Doc. ID 40205TMt01)
1.8 Compliance with Laws and Standards
Covered by instrument conformity and certification.
Refer to the corresponding Operating Manual of the instrument. Refer to section
1.7 “Reference Documents”, 1-2.
1.9 Document Conventions
Cross-references appear as follows—e.g.:
“Refer to section 1.1.1, 1-1”
– 1.1.1 refers to the corresponding section number
– The symbol denotes “page number”
– 1-1 refers to the page number, whereas the first number stands for the
chapter number (chapter 1 - page 1)
Note: For safety conventions and symbols refer to chapter 2 “Safety”,
 2-1.

1 - 4 MIO2 Operating Manual, 394934, en, V3.0
1 - About This Manual
Abbreviations
1.10 Abbreviations
FSE Field service engineer
MIO Monitored Incubator Option

2 - Safety
Safety Message Conventions
MIO2 Operating Manual, 394934, en, V3.0 2 - 1
2 Safety
This chapter describes the safety concept of MIO2, provides general rules of
correct behavior, and warnings concerning hazards associated with the use of the
MIO2.
2.1 Safety Message Conventions
2.1.1 Signal Words
WARNING indicates the possibility of personal injury or even loss of life if the
instructions are not followed.
ATTENTION indicates the possibility of equipment damage, malfunctions or
incorrect process results, if instructions are not followed.
2.1.2 Safety Symbols
General Warning
Warning for hot surface
Read this

2 - 2 MIO2 Operating Manual, 394934, en, V3.0
2 - Safety
General Safety Information
2.2 General Safety Information
WARNING
MIO2 is designed and built in accordance with the present state-of-the-art
technology and the recognized technical safety regulations. Nevertheless, risks to
users, property and the environment can arise if the MIO2 is used without due
care and attention.
The safety of all users and personnel depends on the strict observation of these
safety instructions and awareness of the safety-related warnings provided in this
manual.
Please pay great attention to the following general safety information.
This manual must always be available to all persons performing the tasks
described herein.
Legal regulations, such as local, state and federal laws concerning the use or
application, as well as the handling, of dangerous materials in connection with
the MIO2 must be strictly followed.
The operating company is responsible for defining instructions in accordance
with company procedures and local legal requirements. The instructions
provided by the operating company must be strictly observed.
Observe the correct environmental conditions for storage and operation.
Structural changes to the safety devices are forbidden.
Damaged safety devices must be replaced immediately as described in this
manual.
The MIO2 must not be modified in any way without prior consultation and
written approval of Tecan. Authorized modifications to the system may only be
performed by an FSE certified for the repair and upgrading of the MIO2.
Tecan will reject any claim resulting from unauthorized modifications.
Fire hazard caused by the improper use of the MIO2. The MIO2 should not be
installed in locations where there is a hazard of explosion.
Chemical, biological, and radioactive hazards can be associated with the
substances used or the samples and reagents processed with the MIO2 (e.g.,
during loading and unloading). The same applies to waste disposal.
– Always be aware of possible hazards associated with these substances.
– Use appropriate protective clothing, safety goggles and gloves.
– The handling of substances and the disposal of waste may be subject to
local, state, or federal law, or to regulations with regard to health,
environment, or safety. Strictly observe the corresponding provisions.
Any contamination must be dealt with immediately as described in this
manual.
The user is responsible for ensuring that the MIO2 is always operated under
proper conditions, and that maintenance, service, and repair tasks are
performed with care, on schedule, and only by authorized personnel.
Risk of incorrect measuring results. After system care or maintenance has
been performed, operation must only be resumed after the correct system
operating conditions have been verified.
Always use recommended consumables and original spare parts for
maintenance and repair to assure good system performance and reliability.
Observe all safety instructions of the instrument the MIO2 is used on.
Maximum microplate fill levels are microplate specific and need to be
validated.

2 - Safety
Operating Company
MIO2 Operating Manual, 394934, en, V3.0 2 - 3
Risk of spillage. Microplate filled over the specified fill level will lead to spillage
during operation resulting in contamination.
– Make sure the microplate is not filled more than the specified fill level.
The MIO2 has to be operated with heat-resistant microplates.
Risk of deformation of microplates. Non heat-resistant microplates will get
deformed if heated up.
– Make sure only heat-resistant microplates are used for incubation.
Caustic substances can cause burns and eye injury.
– Avoid exposure to caustic substances.
Avoid the formation and accumulation of flammable vapors.
Avoid the spillage of flammable material.
Prior to using hazardous materials perform a risk assessment.
Consider specific workplace conditions, such as temperature, air ventilation,
electrostatic discharge.
Make sure that the risk is acceptable prior to use of the instrument.
The MIO2 is a heated device. The outer surface may develop temperatures
up to a maximum of 45°C.
– Let the MIO2 cool down hand-hot before removal.
2.3 Operating Company
The operating company must ensure that the MIO2 and in particular the safety
features, function properly and that all the personnel in contact with the instrument
are adequately trained.
Responsibilities Method and process validation.
Defining the processes in compliance with the Standard Operating
Procedures.
Ensuring that installation and operational qualifications (IQ OQs) have been
completed.
Ensuring that all personnel in contact with the MIO2 are adequately trained.
Ensuring the availability of appropriate protective clothing and equipment.
Ensuring the maintenance and safe operation of the MIO2.
Requiring adherence to laboratory safety regulations and directives.
2.4 User Qualification
The laboratory personnel must be fully qualified and trained to operate the MIO2.
The work described in this Operating Manual must only be performed by
authorized personnel with the qualifications prescribed below.
Laboratory personnel must:
have suitable technical training,
be familiar with the laboratory safety regulations and directives,
be familiar with the instructions for the safety elements of the instrument,
use protective clothing and equipment,
be familiar with and adhere to good laboratory practices,

2 - 4 MIO2 Operating Manual, 394934, en, V3.0
2 - Safety
User Qualification
and have read and understood the instructions in the Operating Manual.
Tecan recommends that the operator attends an operator training course. Please
ask the Tecan Customer Service about available courses. Refer to section
10 “Customer Support”, 10-1.
2.4.1 Operator
The operator (lab technician) works for the operating company.
Required Skills No specific application or system knowledge
Command of local languages
Command of English is preferable
The operator has application software access rights allowing him to run methods
and perform system care.
2.4.2 Key Operator
The key operator (application specialist) supports the operating company or works
for the same company.
Required Skills Extensive application knowledge
Limited system knowledge
Command of local languages
Command of English
In-depth knowledge of the corresponding software manual
Responsibilities Instructing the operator
Writing, running and validating methods
Helping the operator to solve problems with the instrument

2 - Safety
Product Safety Signs
MIO2 Operating Manual, 394934, en, V3.0 2 - 5
2.5 Product Safety Signs
Safety signs are affixed for safety purposes. Damaged, lost or illegible safety
signs must be replaced immediately as illustrated. For the meaning of safety
symbols refer to section 2.1.2 “Safety Symbols”, 2-1.
2.6 Decontamination Declaration
In addition to regular system care, and in accordance with standard laboratory
regulations, the MIO2 and its parts and accessories must be thoroughly
decontaminated in the following circumstances:
Before any maintenance or service work is performed on the MIO2 and, in
particular, before an FSE intervention on the MIO2
In the event of accidents (e.g., crash, spillage, etc.)
Before returning the MIO2 or its parts or accessories, to Tecan (e.g., for
repair)
Prior to storage
Prior to disposal
In general, before moving the MIO2 or its parts from its location
The owner of the instrument has full responsibility for the effective
decontamination of all the equipment.
Before any intervention on the MIO2 by an FSE, and before returning the MIO2 or
its parts or accessories to Tecan, the owner of the instrument must complete and
sign the Decontamination Declaration form, confirming that the decontamination
has been performed in accordance with good laboratory practice guidelines.
Contact your local service organization to obtain this form and refer to section
7.1 “Decontamination”, 27-1.
Note: Tecan reserves the right to refuse to deal with any MIO2 or its parts or
accessories that is not accompanied by the Decontamination Declaration form.
Fig. 2-1 Safety signs

2 - 6 MIO2 Operating Manual, 394934, en, V3.0
2 - Safety
Decontamination Declaration

3 - Technical Data
Overview
MIO2 Operating Manual, 394934, en, V3.0 3 - 1
3 Technical Data
3.1 Overview
Overview The MIO2 is a precision instrument designed for automating incubating tasks on a
heated air incubation concept.
Configurations The MIO2 is provided in a 4 and a 6 slot configuration with the following features:
Standard
The standard configuration does not contain the shaking option.
Shaking Incubator
Both incubators can be equipped with a shaking option.
The 6 positions incubator can optionally be equipped with a cooling fan to
provide room temperature incubation in conjunction with shaking.
Fig. 3-1 MIO2
A
B
AIncubator with 6 positions (slots) BIncubator with 4 positions (slots)

3 - 2 MIO2 Operating Manual, 394934, en, V3.0
3 - Technical Data
Product Identification and Appendix Labels
Area of
Application The MIO2 is designated for all Fluent and Freedom EVO instruments—except for
Freedom EVO 75 that is incompatible with MIO2 with 4 positions.
Delivery It is recommended that theMIO2 is only delivered and installed by Tecan
authorized field service engineers.
3.2 Product Identification and Appendix Labels
6 Slot MIO
Fig. 3-2 MIO2, 6 positions, product identification and appendix labels
Calibrated at XY °C
INCUBATOR MIO2 XY°C
300YYYYY
24VDC
150W
T8AFuse
P
U,f
REF
Model
1001
SN 300YYYYY 1001
CB
A
D
E
AProduct identification label
BCalibration label
CConfiguration label
DMonitored Incubator Option
ECaution label

3 - Technical Data
Product Identification and Appendix Labels
MIO2 Operating Manual, 394934, en, V3.0 3 - 3
4 Slot MIO
Product
Identification
Label
The product identification label (A) provides the following instrument details Fig. 3-
2, 3-2:
Calibration
Label The calibration label (B) states the temperature the MIO2 was calibrated and
tested for Fig. 3-2, 3-2.
Configuration
Label The configuration label (C) Fig. 3-2, 3-2 specifies the resistance of the slots.
Fig. 3-3 MIO2, 4 positions, product identification and appendix labels
Calibrated at XY °C
INCUBATOR MIO2 4-Slot XY°C
300YYYYY
24VDC
150W
T8AFuse
P
U,f
REF
Model
1001
SN
300YYYYY 1001
CB
A
D
E
AProduct identification label
BCalibration label
CConfiguration label
DMonitored Incubator Option
ECaution label
Tab. 3-1 MIO2 product identification label
Note Definition
Identification data Model: Product name / model
REF: Reference number / Revision / Bar code
Manufacturing date
SN: Serial number
Technical data Voltage: supply voltage (Volts), 24 V DC
Power: power consumption (Watts) 150 W
Fuse: T8A 250V
Manufacturer’s name and address:
Tecan Schweiz AG, CH-8708 Männedorf
Barcode Barcode: Serial number and product type

3 - 4 MIO2 Operating Manual, 394934, en, V3.0
3 - Technical Data
Dimensions and Weights
3.3 Dimensions and Weights
6 Slot MIO
Dimensions
Weights
Fig. 3-4 Dimensions of the MIO2, 6 positions
W W
Lb
La
H H
A B
AMIO2 standard BMIO2 with shaker option
Tab. 3-2 Dimensions of the MIO2, 6 positions
Dimensions Value
La Length of standard configuration 213 mm (8.38 in.)
Lb Length with shaking assembly / fan option
configuration 265 mm (10.43 in.)
W Overall width 130.5 mm (5.13 in.)
H Overall height 267.5 mm (10.53 in.)
Tab. 3-3 Weight of configurations, 6 positions
Type Value
Standard configuration weight 3.5 kg (7.7 lbs.)
Shaking assembly configuration 4.8 kg (10.6 lbs.)
Shaking assembly with fan option configuration 4.9 kg (10.8 lbs.)

3 - Technical Data
Dimensions and Weights
MIO2 Operating Manual, 394934, en, V3.0 3 - 5
4 Slot MIO
Dimensions
Weights
Fig. 3-5 Dimensions of the MIO2, 4 positions
W
La
H
A
W
Lb
H
B
AMIO2 standard configuration BMIO2 with shaker option
Tab. 3-4 Dimensions of the MIO2, 4 positions
Dimensions Value
La length of standard configuration 228 mm (8.98 in.)
Lb length with shaking assembly / fan option
configuration 278 mm (10.95 in.)
W Overall width 130.5 mm (5.13 in.)
H Overall height 267.5 mm (10.53 in.)
Tab. 3-5 Weight of configurations, 4 positions
Type Value
Standard configuration weight 3.6 kg (7.9 lbs.)
Shaking assembly configuration 4.6 kg (10.1 lbs.)

3 - 6 MIO2 Operating Manual, 394934, en, V3.0
3 - Technical Data
Operating Range
3.4 Operating Range
6 Slot MIO
4 Slot MIO
Tab. 3-6 Operating ranges, 6 positions
Number of Slots 6
Incubating tempera-
ture range from (actual room temperature + 5°C) to 60°C from (actual room temperature + 9°F) to 140°F
Accuracy of chamber
temperature max. ±0.5°C at 37°C (max. ±0.9°F at 99°F)
max. ±0.5°C at 46°C (max. ±0.9°F at 115°F)
max. ±2.0°C at 60°C (max. ±3.6°F at 140°F)
Accuracy of tempera-
ture in microplate in
the chamber
max. ±1°C at 37°C (max. ±1.8°F at 99°F)
max. ±1°C at 46°C (max. ±1.8°F at 115°F)
max. ±2°C at 60°C (max. ±3.6°F at 140°F)
Warm-up time from
20°C to 37°C max. 20 min.
Warm-up time from
20°C to 45°C max. 20 min.
Warm-up time from
20°C to 60°C max. 30 min.
Shaking / rotation fre-
quency 1–8.5 Hz
Linear shaking max. 2 mm amplitude in Y-dimension
Tab. 3-7 Operating ranges, 4 positions
Number of Slots 4
Incubating tempera-
ture range from (actual room temperature + 5°C) to 60°C from (actual room temperature + 9°F) to 140°F
Accuracy of chamber
temperature max. ±1°C at 37°C (max. ±1.8°F at 99°F) max. ±1°C at 46°C (max. ±1.8°F at 115°F)
max. ±2°C at 60°C (max. ±3.6°F at 140°F)
Accuracy of tempera-
ture in microplate in
the chamber
max. ±1°C at 37°C (max. ±1.8°F at 99°F)
max. ±1°C at 46°C (max. ±1.8°F at 115°F)
max. ±2°C at 60°C (max. ±3.6°F at 140°F)
Warm-up time from
20°C to 37°C max. 30 min.