Thermo Scientific Invitrogen PowerEase Touch PS0350 User manual

For Research Use Only. Not for use in diagnostic procedures.
PowerEase™Touch 350W Power Supply
Catalog Numbers PS0350 (115 VAC), PS0351 (230 VAC), PS0352 (230 VAC Australia), PS0353 (230
VAC China)
Publication Number MAN0019503
Revision A.0

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Revision history: Pub. No. MAN0019503
Revision Date Description
A.0 28 August 2020 New document.
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Contents
■CHAPTER 1 Product information .................................................. 5
Product description ............................................................. 5
Product contents ................................................................ 7
■CHAPTER 2 Methods ............................................................... 9
Procedural guidelines ............................................................ 9
Install the power supply ......................................................... 10
Programming options ........................................................... 10
Menu navigation ............................................................... 11
Homescreen ................................................................... 11
Set up a run ................................................................... 12
Constant current ........................................................... 13
Constant Power ........................................................... 13
Custom methods .............................................................. 13
Select a pre-programmed method ................................................ 14
Manage methods .............................................................. 16
Save a method ............................................................ 16
Edit a method ............................................................. 17
Delete a method ........................................................... 18
Change the name of a saved method ........................................ 18
Add a step ................................................................ 19
Delete a step .............................................................. 19
Save a program ........................................................... 19
Instrument display during a run .................................................. 20
Run screen features ........................................................ 20
Method Settings screen features ............................................ 21
Instrument display after run completion .......................................... 22
Settings ....................................................................... 22
Settings screen features .................................................... 23
PowerEase™Touch 350W Power Supply User Guide 3

■APPENDIX A Troubleshooting .................................................... 25
■APPENDIX B Repair and maintenance .......................................... 27
Encountering Problems ......................................................... 27
Replace the fuse ............................................................... 27
■APPENDIX C Electrical parameters for electrophoresis ........................ 29
Ohm’s Law and Joule’s Law ..................................................... 29
Resistance .................................................................... 29
Voltage ....................................................................... 29
Current ....................................................................... 29
Power ........................................................................ 30
■APPENDIX D General safety ...................................................... 31
Instrument safety .............................................................. 31
Avoid electrical shock ...................................................... 31
Avoid damage to the instrument ............................................. 31
Explanation of symbols and warnings ............................................ 32
Symbols and warnings ..................................................... 32
Product regulatory compliance .................................................. 33
Federal Communications Commission Advisory ............................... 33
Environmental design compliance ........................................... 33
■APPENDIX E Product specifications ............................................. 34
Product specifications .......................................................... 34
■APPENDIX F Documentation and support ...................................... 36
Customer and technical support ................................................. 36
Limited product warranty ........................................................ 36
Contents
4PowerEase™Touch 350W Power Supply User Guide

Product information
Product description
The PowerEase™Touch 350W Power Supply is a high throughput power supply
that uses a touch-screen user interface. This power supply is ideal for DNA/RNA
electrophoresis, SDS-PAGE, and native PAGE.
The power supply unit has the following features:
•Touch screen LCD display and user interface with clear menu prompts for easy
use by hand or stylus
•Maximum output of 300 V, 3 A, and 350 W (for complete specifications, see
Product Specifications)
•Four sets of output terminals allow running of multiple electrophoresis units
•Pop-up feet allow change to screen viewing angle
•Sturdy polyurethane feet and stackable housing design allow stacking of power
supplies
•Running modes include Constant Voltage, Constant Power, or Constant Current
•Programming capabilities for limiting voltage (V), current (mA) and power (W)
•Method programming flexibility (up to 100 methods, 20 steps per method, 999
minutes per step)
•Pause button for editing method parameters during run
•User adjustable device settings such as beep volume, screen brightness and
sleep mode duration
•Automatic safety features such as No Load, Over Temperature, Over Voltage,
Over Current, Load Change, and Ground Leak detection
This manual describes the setup and operation of the PowerEase™Touch 350W
Power Supply and includes important information on safety and maintenance of the
unit.
1
PowerEase™Touch 350W Power Supply User Guide 5

Figure 1 Front view of PowerEase™Touch 350W Power Supply
Figure 2 Rear view of PowerEase™Touch 350W Power Supply
Figure 3 USB port dust cover (Rear view of PowerEase™Touch 350W Power Supply)
The USB port is shipped with a plastic dust cover for protection during shipment. This cover must
be removed prior to port use. Remove by gently prying o with a small flat blade screwdriver.
Chapter 1 Product information
Product description
1
6PowerEase™Touch 350W Power Supply User Guide

Product contents
On receipt, examine the unit carefully for any damage incurred during transit. Any
damage claims must be filed with the carrier, and keep the supplied box for
inspection. The warranty does not cover in-transit damage.
IMPORTANT! Some of the power cords appear similar; users should utilize the cord
approved for their country/region. North America customers should use the Type B
power cord; Japan customers should use the JIS 8303 power cord; China customers
should use the GB 15934-2008 power cord; Australia/New Zealand customers
should use the Type I power cord.
Table 1 Cat. No. PS0350 (115 VAC)
Component Quantity Regulatory mark(s) on
electrical plug
PowerEase™Touch 350W
Power Supply 1 each
Extra Fuse 1 each
Type B Power Cord 1 each CSA
JIS 8303 Power Cord 1 each PSE
USB Cover 1 each
Quick Reference Card 1 each
Certificate of Performance 1 each
Table 2 Cat. No. PS0351 (230 VAC)
Component Quantity Regulatory mark(s) on
electrical plug
PowerEase™Touch 350W
Power Supply 1 each
Extra Fuse 1 each
Type G Power Cord 1 each ASTA
Type CEE 7/7 Power Cord 1 each CE
USB Cover 1 each
Quick Reference Card 1 each
Certificate of Performance 1 each
Chapter 1 Product information
Product contents 1
PowerEase™Touch 350W Power Supply User Guide 7

Table 3 Cat. No. PS0352 (230 VAC Australia)
Component Quantity Regulatory mark(s) on
electrical plug
PowerEase™Touch 350W
Power Supply 1 each
Extra Fuse 1 each
Type I Power Cord 1 each NSW 18392
USB Cover 1 each
Quick Reference Card 1 each
Certificate of Performance 1 each
Table 4 Cat. No. PS0353 (230 VAC China)
Component Quantity Regulatory mark(s) on
electrical plug
PowerEase™Touch 350W
Power Supply 1 each
Extra Fuse 1 each
Type GB 15934-2008 Power
Cord 1 each CCC
USB Cover 1 each
Quick Reference Card 1 each
Certificate of Performance 1 each
Chapter 1 Product information
Product contents
1
8PowerEase™Touch 350W Power Supply User Guide

Methods
Procedural guidelines
We recommend that you carefully review these guidelines before operating the
instrument.
•To ensure safe, reliable operation, always operate the PowerEase™Touch 350W
Power Supply in accordance with the manufacturer’s instructions.
•Avoid exposing the power supply to liquids as these may damage the unit and
void the warranty.
•IMPORTANT! Do not use the PowerEase™Touch 350W Power Supply at
its maximum electrical load limits. Variations in buer conditions can result
in exceeding the power supply’s maximum voltage, current, or power output
capacity and produce undesirable variations in performance.
•When using this or any electrophoresis product, we recommend that you adhere
to the protocols given in the electrophoresis product manuals.
For best results:
•We recommend using the Constant Voltage Mode for protein gels or blots.
Note: For most electrophoresis methods, resistance increases throughout the
run. Limiting the voltage allows for an improving margin of safety over time
because the current and power decrease through the run. Also, the same
voltage can be used regardless of the number or the thickenss of gels being
electrophoresed.
•Avoid running samples of widely diering salt concentrations or sample buers
at the same time or on the same gel.
Note: Variations in conductivity due to dierences in buer salt concentrations
can aect the run of all the samples run at the same time.
•Properly prepare your samples.
2
PowerEase™Touch 350W Power Supply User Guide 9

Install the power supply
1. Check the label located on the bottom of the power supply to ensure that the
unit is compatible with locally provided voltage.
2. Place the power supply on a level laboratory bench and position it such that
the On/O switch and the AC inlet at the rear of the power supply are easily
accessible. Keep the area around the power supply clear to ensure proper
ventilation of the unit.
3. Ensure the On/O switch located at the rear of the power supply is in the O
position.
4. Attach the power cord to the AC inlet of the power supply.
5. Plug the power supply into an appropriate electrical outlet (use properly
grounded AC outlets and power cords).
6. Connect the leads from the electrophoresis unit to the power supply by inserting
the positive lead (+) into the red output jack and the negative lead (-) into the
black output jack.
7. Turn the AC power switch at the back of the power supply On.
Programming options
The PowerEase™Touch 350W Power Supply can be programmed to run single-step
and multi-step methods. Up to 100 user defined methods can be saved; up to 20
steps can be saved for each multi-step method.
This power supply is also equipped with several pre-programmed gel electrophoresis
and transfer methods. These methods include voltage and time parameters required
to run and transfer many pre-cast gel products from Thermo Fisher Scientific.
This power unit has a touch sensitive screen, so the selection of on-screen buttons
and parameters is accomplished by touching the screen at the location of the item to
be selected.
Chapter 2 Methods
Install the power supply
2
10 PowerEase™Touch 350W Power Supply User Guide

Menu navigation
The PowerEase™Touch 350W Power Supply uses several screen icons that simplify
menu navigation.
Icon Description
The Home icon returns to the Home screen (Figure 4).
The back arrow returns to the previous screen.
Left and right arrows indicate that an additional screen is available.
Touch the arrow todisplay the additional screen.
Touch this icon to close the current screen
Touch this icon to navigate to the Settings screen (available on the
Home screen only).
Homescreen
On power up, the Home screen is displayed (see Figure 4).
The Set up run option will appear on the Home screen when the power supply is
switched on for the first time or it if is switched on after running an unsaved custom
method (see below).
The Last run option will appear on the Home screen when the power supply
is switched on after having previously run a saved custom method or a pre-
programmed method. It will also be shown after an un-saved custom method is run
until the power supply is shut o.
Figure 4 Home screens
The following options can be selected from the Home screen:
•Select Set up run to program a new run method.
•Select Custom methods to choose a previously saved run method.
•Select Pre-programmed methods to choose a pre-loaded run method.
Chapter 2 Methods
Menu navigation 2
PowerEase™Touch 350W Power Supply User Guide 11

Set up a run
1. Switch the power switch located at the rear of the unit to the On position.
2. Touch Set up run.
3. In the Method Type screen touch either Constant voltage, Constant current,
or Constant power.
Figure 5 Method Type screens
4. Edit the run parameters (Volts, Time, Amps, and Watts) by touching the
parameter on the screen to bring up the Run parameter edit screen.
Default maximum values are 300 V, 3,000 mA, and 350 W.
Note: When using constant voltage or constant power, it is recommended
that the maximum default settings not be changed to avoid unwanted power
limitations during the run.
5. Enter the parameter value required, then touch Enter to advance with the newly
changed run parameter displayed.
6. (Optional) Add or delete extra steps by touching Actions.
Chapter 2 Methods
Set up a run
2
12 PowerEase™Touch 350W Power Supply User Guide

7. Touch Start run to begin the run. When the run begins, the Run screen will
display (See Figure 12).
Note: If the run needs to be stopped or paused before completion, touch Stop
or Pause. If run parameters need to be edited during the run, pause the run,
enter changes, then touch Resume. When resumed, the run time will continue
from where it was paused.
8. When the run is complete, the Run Complete screen will be displayed (see
Figure 13).
If the method used is a custom method that has not yet been saved, the Run
Complete screen will display a Save as option. If the method is a saved or
pre-programmed method, the Save as option will not be present. This screen
also displays an option to navigate to the Home screen or to repeat the method.
Constant current
Discontinuous buer systems and, to a lesser extent, continuous systems increase
resistance during the run. With the current limiting setting on the PowerEase™Touch
350W Power Supply, the voltage will increase as resistance increases to satisfy
Ohm’s law (V=IR). If no voltage limit is set and a local fault condition occurs, such as
a poor connection, very high local resistance may cause the voltage to increase to
the maximum capacity of the power supply. This may lead to local overheating and
damage to the electrophoresis cell or create unsafe conditions.
Note: When operating under constant current conditions, it is recommended to set a
voltage limit on the power supply slightly above the maximum expected for the run.
Constant Power
Power is a function of voltage and current P=IV. For a typical gel run at constant
power, the current will decrease during the run, causing the voltage to increase. The
power limiting function may be used when running sequencing gels to remove the
ammonium persulfate from the wells and to heat the gel to an optimal temperature for
DNA separations.
Custom methods
Select Custom Methods on the Home screen to bring up the Custom Methods
screen. From here, the Saved Methods or Method Type screen can be selected (see
Figure 6).
The Saved Methods screen displays methods that have been previously saved
(shown in alphabetical order). Selecting a method brings up the run screen for the
method, allowing the method to be run or edited. The saved method can be edited
by touching any of the run parameter fields.
The Method Type screen displays method types that can be entered (see Figure 5).
Chapter 2 Methods
Custom methods 2
PowerEase™Touch 350W Power Supply User Guide 13

Figure 6 Custom Methods screen options
Select a pre-programmed method
1. On the Home screen touch Pre-programmed Methods.
2. In the Pre-programmed Methods screen, touch either Mini gels, Midi gels, or
Western blots (see Figure 7).
•Selecting Mini gels brings up a list of preloaded methods for
electrophoresing mini gels sold by Thermo Fisher Scientific, Inc.
•Selecting Midi gels brings up a list of preloaded methods for
electrophoresing midi gels sold by Thermo Fisher Scientific, Inc.
•Selecting Western blots brings up a list of preloaded methods for
transferring above gels to Western blots
3. To view method options not available on the first screen, gently swipe up with
your finger as you would with a smart phone.
4. Touch a method to bring up the Run screen for that method.
These screens display default running conditions (voltage, and time) for the
pre-programmed method selected. The current and power settings shown are
the power supply maximums.
Chapter 2 Methods
Select a pre-programmed method
2
14 PowerEase™Touch 350W Power Supply User Guide

5. Edit the method by touching Edit, then save the method using a new name (see
Figure 9) or begin the run by touching Start run.
Note: For a pre-programmed method, only the run time can be edited until it is
saved as a custom method.
IMPORTANT! For a given gel buer type (Tris-Glycine, Bis-Tris, etc.) and run
voltage, gel run times are dependent on several factors. Factors such as running
buer concentration and temperature, type of gel tank used (because of the
electrical path distance between anode and cathode wires), gel separation
gradient and level of running buer above the wells can aect run times to
some degree. So, the exact run time for your gel may be slightly dierent
than the default value given by the pre-programmed method. The default run
times shown in mini gel pre-programmed methods assume the use of the Mini
Gel Tank (Thermo Scientific™(Cat. No. A25977)). Midi gel run times assume
the use of the SureLock™Tandem Midi Gel Tank (Thermo Scientific™(Cat. No.
STM1001)).
Figure 7 Pre-programmed Methods Screens
For example: The pre-programmed method for running NuPAGE™Bis-Tris gels with
MES running buer is shown in Figure 8.
Once the run has started, the Run screen will display real time parameter values.
To view run parameters as they were set, touch the right arrow to view the Method
Settings screen (see Figure 12).
Figure 8 Pre-programmed Method for NuPAGE™Bis-Tris Mini Gels
Chapter 2 Methods
Select a pre-programmed method 2
PowerEase™Touch 350W Power Supply User Guide 15

Manage methods
New custom methods can be saved and pre-programed methods can be edited and
then saved under a new (custom) name. Methods can be single step or have up to 20
steps per method. Up to 100 custom methods can be saved (Figure 10).
Save a method
1. Save a new method by touching Actions on the Method Run screen (see
Figure 9).
2. Save an edited pre-programmed method by touching Edit.
3. Touch the Name of method field in the Save As screen to bring up a keyboard
that will allow entry of the method name.
Method names can contain a maximum of 14 characters. Keyboards displaying
numbers and lower-case letters are accessed using buttons in the lower left
corner of the screen.
4. Enter the name, touch Enter, then touch Save.
Note: Do not save custom methods with the same name as a pre-programmed
method.
Chapter 2 Methods
Manage methods
2
16 PowerEase™Touch 350W Power Supply User Guide

(New method) (Pre-programmed method)
Figure 9 Screens for saving methods
Edit a method
1. To change any of the run parameters in a method, touch the data field for the
parameter you wish to change. Selecting a run parameter will bring up its Edit
screen.
2. Enter the parameter value required, then press Enter.
Note: Do not save custom methods with the same name as a pre-programmed
method.
Chapter 2 Methods
Manage methods 2
PowerEase™Touch 350W Power Supply User Guide 17

Delete a method
1. On the Home screen, touch Custom methods, then touch Saved methods
(Figure 4).
This will bring up the Saved Methods screen.
2. Touch Manage to bring up the Manage Saved Methods screen.
The methods are listed in alphabetical order.
3. Select the method to be deleted by touching its row in the list of displayed
methods, then press Delete.
If several methods have been saved, swipe up as you would with a smart phone
to scroll down the list.
Figure 10 Managing saved methods
Change the name of a saved method
1. Touch Actions, then Save As (see Figure 9).
This will bring up the Save As screen with the current method name as the
header.
2. Touch the method name and use the keyboard to change the name.
3. Touch Enter to save.
Chapter 2 Methods
Manage methods
2
18 PowerEase™Touch 350W Power Supply User Guide

Add a step
1. Touch Actions, then Add step (see Figure 9).
This will bring up the Constant Voltage, Constant Current, or Constant Power
screen, depending on the method type selected for the method (see Figure 5).
2. Repeat for additional steps.
The step number will be displayed below the constant parameter value (see
Figure 11). To view adjacent steps when viewing the constant parameter screen,
touch the left arrow (previous step) or right arrow (next step).
3. When all of the steps have been entered, touch Start run to initiate the run.
To
previous
step
To
next
step
Figure 11 Method step number location
Delete a step
1. Navigate to the step to be deleted using the left and right arrows on the
Constant parameter screen (see Figure 11).
2. Touch Actions, then Delete step.
Save a program
1. Touch Actions, then Save As.
2. Touch the name field in the Save As screen to bring up the keyboard, then enter
the method name.
3. Touch Enter, then touch Save.
Chapter 2 Methods
Manage methods 2
PowerEase™Touch 350W Power Supply User Guide 19

Instrument display during a run
After touching Start run, the run is initiated, and the Run screen is displayed for the
selected method.
As an example, a Run screen and a Method Settings screen for a constant voltage
custom method is shown in the following figure:
Figure 12 Run screen and Methods Settings screen
1Method name
2Center dial with digital timer countdown, step number and visual countdown indicator
3Real time output parameters (A, V, W)
4Crossover indicator
5Stop and Pause buttons
6Right arrow for navigation to method settings screen
Run screen features
Method name
The name of the method will be displayed at the top of the screen. If the method is
an un-named custom method, the name displayed will be the method type (Constant
Voltage, Constant Current, or Constant Power). The method displayed in Figure 12 is
a custom constant voltage method.
Center dial
The center dial displays:
•Countdown timer in hours, minutes, and seconds (example: 01:2735 = 1 hour, 27
minutes, 35 seconds). The timer resets for each step in a multi-step method.
•A colored visual indicator for time remaining in the method
•“Running” status
•The method step currently being run (for multistep methods only)
Chapter 2 Methods
Instrument display during a run
2
20 PowerEase™Touch 350W Power Supply User Guide