Thermo Scientific Dionex DRS 600 User manual

For Research Use Only. Not for use in diagnostic procedures.
6
Dionex DRS 600 Suppressor
Dionex ERS 500e Suppressor
Dionex ERS 500 Carbonate Suppressor
031956 Revision 13 •March 2018
User Manual User Manual

Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 2 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
Product Manual
for
Dionex Anion Dynamically Regenerated Suppressor 600
(Dionex ADRS 600 (4 mm), Item # 088666)
(Dionex ADRS 600 (2 mm), Item # 088667)
Dionex Cation Dynamically Regenerated Suppressor 600
(Dionex CDRS 600 (4 mm), Item # 088668)
(Dionex CDRS 600 (2 mm), Item # 088670)
Dionex Anion Electrolytically Regenerated Suppressor 500e
(Dionex AERS 500e (4 mm), Item # 302661)
(Dionex AERS 500e (2 mm), Item # 302662)
Dionex Cation Electrolytically Regenerated Suppressor 500e
(Dionex CERS 500e (4 mm), Item # 302663)
(Dionex CERS 500e (2 mm), Item # 302664)
Dionex Anion Electrolytically Regenerated Suppressor 500 for
Carbonate Eluents
(Dionex AERS 500 Carbonate (4 mm), Item # 085029)
(Dionex AERS 500 Carbonate (2 mm), Item # 085028)

Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 3 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
© 2018 Thermo Fisher Scientific Inc. All rights reserved.
MASTERFLEX C/L is a registered trademark of Barnant Company. All other trademarks are the property of
Thermo Fisher Scientific Inc. and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product
operation. This document is copyright protected and any reproduction of the whole or any part of this document is
strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this document is
for reference purposes only. System configurations and specifications in this document supersede all previous
information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-free and
assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from
any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Revision History:
Revision 08, April 24, 2013, Rebranded for Thermo Scientific. Product name changed from Dionex SRS 300 to
Dionex ERS 500.
Revision 09, November 2013, Updated External Water Mode flow rate recommendations, Updated hydration
procedure.
Revision 10, August 2015, Added support for the Neutralization mode. Added support for Carbonate Eluents.
Revision 11, January 2017, Updated the recommended suppressor hydration procedure. Edited for length and
clarity. Added Dionex ERS 500e product.
Revision 12, January 2018, Added information to support the release of Dionex DRS 600 and the discontinuance
of Dionex ERS 500.
Revision 13, March 2018, Added information about the Firmware, software and programming requirements to
operate Dionex DRS 600 with Thermo Scientific Dionex ICS 6000 and Thermo Scientific Dionex
Integrion HPIC systems

Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 4 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and other
special notices appear in boxes.
Safety and special notices include the following:
Indicates a potentially hazardous situation which, if not avoided, could result in death or
serious injury.
Indicates a potentially hazardous situation which, if not avoided, could result in damage
to equipment.
Indicates a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury. Also used to identify a situation or practice that may seriously damage
the instrument, but will not cause injury.
Indicates information of general interest.
IMPORTANT
Highlights information necessary to prevent damage to software, loss of data, or invalid
test results; or might contain information that is critical for optimal performance of the
system.
Tip
Highlights helpful information that can make a task easier.
SAFETY
!
WARNING
!
CAUTION
!
NOTE
!

Contents
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 5 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
Contents
1. Introduction............................................................................................................................. 8
1.1 Dynamically Regenerated Suppressor............................................................................................9
1.2 DRS 600 Operation ........................................................................................................................9
1.3 Suppressor Design........................................................................................................................10
1.4 Overview of Suppression and Neutralization Modes...................................................................14
1.4.1 Mode of Operation Selection ..............................................................................................................14
1.4.2 The AutoSuppression Recycle Mode..................................................................................................16
1.4.3 The AutoSuppression External Water Mode.......................................................................................16
1.4.4 The Chemical Suppression Mode........................................................................................................17
1.4.5 The MPIC Suppression Mode.............................................................................................................17
1.4.6 The Neutralization Mode ....................................................................................................................17
1.5 Shipment and Storage...................................................................................................................19
1.5.1 Shipment .............................................................................................................................................19
1.5.2 Storage ................................................................................................................................................19
2. Installation............................................................................................................................. 20
2.1 System Requirements...................................................................................................................20
2.2 Electrolytically Regenerated Suppressor Control.........................................................................22
2.2.1 Dionex Reagent-Free Controller (Dionex RFC) .................................................................................23
2.3 Back Pressure Coils for the Dionex DRS 600, Dionex ERS 500e and
Dionex AERS 500 Carbonate.......................................................................................................23
2.3.1 Assembly.............................................................................................................................................24
2.4 Gas Separator Waste Tube for the Dionex ERS 500....................................................................25
2.4.1 Assembly.............................................................................................................................................25
2.5 Suppressor Voltage Setting ..........................................................................................................25
2.5.1 Manually setting the voltage ...............................................................................................................26
2.6 Suppressor Current Setting...........................................................................................................28
2.6.1 Calculating the Optimum Current Setting...........................................................................................28
2.7 Operating DRS 600 suppressor with Chromeleon........................................................................30
2.7.1 Firmware and Software Requirements................................................................................................30
2.7.2 Programming the DRS 600 Suppressor with Chromeleon..................................................................31
2.7.3 Operating the DRS 600 with a New Method with Integrion and ICS 6000 ........................................38
2.7.4 Operating the DRS 600 with ICS 6000 with the Legacy Instrument Method.....................................38
2.7.5 Operating the DRS 600 with Integrion with the Legacy Instrument Method.....................................40
3. Operation............................................................................................................................... 43
3.1 Chemical Purity Requirements.....................................................................................................43
3.1.1 Inorganic Chemicals............................................................................................................................43
3.1.2 Solvents...............................................................................................................................................43
3.1.3 Deionized Water..................................................................................................................................43

Contents
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 6 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
3.2 Start-up .........................................................................................................................................44
3.2.1 Hydration ............................................................................................................................................45
3.2.2 Back Pressure Coil Pressure Test........................................................................................................46
3.2.3 Quick Back Pressure Check................................................................................................................47
3.3 Plumbing for the AutoSuppression Recycle Mode Operation......................................................47
3.3.1 Eluent Flow Path Connections in the AutoSuppression Recycle Mode..............................................48
3.3.2 Regenerant Flow Path Connections in the AutoSuppression Recycle Mode ......................................48
3.3.3 Installation in Thermal Chamber.........................................................................................................49
3.4 Plumbing for the AutoSuppression External Water Mode Operation..........................................50
3.4.1 Eluent Flow Path Connections for the AutoSuppression External Water Mode.................................51
3.4.2 Regenerant Flow Path Connections in the AutoSuppression External Water Mode...........................51
3.4.3 Regenerant Flow Path Connections in the AutoSuppression
External Water Mode with Peristaltic Pump.......................................................................................52
3.4.4 Installation in Thermal Chamber.........................................................................................................53
3.4.5 Dionex SRD-10 Suppressor Regenerant Detector ..............................................................................53
3.5 Plumbing for Chemical Suppression Mode Operation.................................................................53
3.6 Plumbing for MPIC Suppression Mode of Operation..................................................................53
3.6.1 Eluent Flow Path Connections in MPIC Suppression Mode...............................................................53
3.6.2 Regenerant Flow Path Connections in MPIC Suppression Mode
Using Pressurized Water Delivery System..........................................................................................54
3.6.3 MPIC Suppression Mode Operation ...................................................................................................54
3.7 Plumbing for Neutralization Mode Operation..............................................................................56
3.7.1 Sample Liquid Line Connections for Dionex DRS 600 in Neutralization Mode................................56
3.7.2 Regenerant Liquid Line Connections for Dionex DRS 600 in Neutralization Mode..........................56
3.7.3 Pump Trap Column Installation and Regeneration .............................................................................57
3.7.4 Neutralization Mode Operation...........................................................................................................58
3.8 Dionex DRS 600, Dionex ERS 500e or Dionex AERS 500 Carbonate Storage..........................59
3.8.1 Short-Term Storage (3 to 7 days)........................................................................................................59
3.8.2 Long-Term Storage (More than 7 days)..............................................................................................59
4. Example Neutralizer Mode Applications............................................................................ 60
4.1 System Blank................................................................................................................................60
4.1.1 Anion System Blank ...........................................................................................................................60
4.1.2 Cation System Blank...........................................................................................................................61
4.2 Dionex CDRS 600 Conditioning..................................................................................................61
4.3 System Calibration .......................................................................................................................62
4.3.1 Analysis of Acid Samples Containing High Concentrations of Transition Metals .............................62
4.4 Anion Example Applications........................................................................................................63
4.4.1 Dionex IonPac®AS11 Gradient..........................................................................................................63
4.4.2 Dionex IonPac AS11 Gradient Analysis of 10% Sodium Hydroxide .................................................64
4.4.3 Dionex IonPac AS12A Isocratic Analysis of 25% Sodium Hydroxide...............................................66
4.4.4 Dionex IonPac AS12A Isocratic Analysis of 20% Ammonium Hydroxide........................................68
4.4.5 Dionex IonPac AS12A Isocratic Analysis of 25% Tetramethylammonium Hydroxide .....................69
4.4.6 IonPac AS12A Isocratic Analysis of 25% Tetrabutylammonium Hydroxide.....................................70
4.5 Cation Example Applications.......................................................................................................71
4.5.1 Dionex IonPac CS12A Blank and Standard Analysis.........................................................................71
4.5.2 Dionex IonPac CS12A Analysis of 24% Sulfuric Acid ......................................................................72

Contents
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 7 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
4.5.3 Dionex IonPac CS12A Analysis of 25% Acetic Acid.........................................................................73
4.5.4 Dionex IonPac CS12A Analysis of 10% Hydrofluoric Acid ..............................................................74
4.5.5 Dionex IonPac CS12A Analysis of 22% Phosphoric Acid .................................................................75
5. Troubleshooting Guide......................................................................................................... 76
5.1 Electrolytically Regenerated Suppressor Operational Status Displays Alarm State ....................76
5.2 Small or Increasing Analyte Peak Areas......................................................................................77
5.2.1 Suppressor Chemical Regeneration Steps...........................................................................................77
5.2.2 Suppressor Electrolytic Regeneration Steps........................................................................................78
5.2.3 Full or Extended Regeneration:...........................................................................................................78
5.3 High Background Conductivity....................................................................................................80
5.4 Drifting Baseline...........................................................................................................................80
5.5 Noisy Baseline..............................................................................................................................81
5.6 Decreased Sensitivity ...................................................................................................................82
5.6.1 Decreased Sensitivity for all analytes .................................................................................................82
5.6.2 Decreased Sensitivity for Magnesium and Calcium only
(CDRS 600 and CERS 500e suppressors)...........................................................................................82
5.7 System Back Pressure Increases Over Time ................................................................................83
5.8 Liquid Leaks.................................................................................................................................83
5.9 Poor or unstable recovery of certain peaks...................................................................................84
5.10 Peaks and spikes in the absence of an injection ...........................................................................84
5.11 Low Sample Response (Neutralization Mode Only)....................................................................85
5.12 Low Neutralization Capacity (Neutralization Mode Only)..........................................................85
6. Dionex DRS 600, Dionex ERS 500e and Dionex AERS 500
Carbonate Suppressor Cleanup........................................................................................... 86
6.1 Metal Contaminants or Precipitates..............................................................................................86
6.2 Organic Contaminants..................................................................................................................87
Appendix A –Current Settings................................................................................................... 88
A.1. Optimum Current Settings for Common Eluents; Dionex AERS 500 (4 and 2 mm)
Legacy Mode, and Dionex AERS 500e (4 and 2 mm).................................................................88
A.2. Optimum Current Settings for Common Eluents; Dionex AERS 500 Carbonate
(4 and 2 mm).................................................................................................................................90
A.3. Optimum Current Settings for Common Eluents; Dionex CERS 500 (4 mm and 2 mm)
and Dionex CERS 500e (4 mm and 2 mm)..................................................................................91

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 8 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1. Introduction
Suppressor: The role of a suppressor in Ion Chromatography is to remove the eluent and sample
counter-ions and replace this with regenerant ions thereby converting the eluent to a weakly
dissociated form prior to detection. Detection of analyte ions particularly with conductivity
detection is therefore feasible against a low background. The suppressor not only reduces the
background signal but also the noise associated with the signal. Furthermore, the analytes are
converted to the more conductive acid or base form, which enhances the signal, particularly for
fully dissociated species. Thus, overall improvement in detection limits, as observed from the
signal to noise ratio, is achieved. When compared to applications that do not use a suppressor,
i.e., single column ion chromatography, the improvement in noise with suppressed ion
chromatography far exceeds the noise performance of single column chromatography
applications. Hence the suppressor has become an integral part of the ion chromatography
instrument.
The suppressors from Thermo Fisher Scientific are designed for continuous operation and do not
require any switching or offline regeneration. Furthermore, the standards and the samples are
always exposed to the same suppressor device when pursuing ion analysis, thus ensuring that the
analytical parameters are consistent between calibration and analysis. There are two types of
suppressors offered for continuous operation; electrolytically regenerated suppressors and
chemically regenerated suppressors. The electrolytic suppressors operate continuously with a
water source as a regenerant. In the recycle mode of operation the water source is derived from
the suppressed eluent post-detection, thereby making suppressor operation simple. The chemical
suppressors operate continuously with an external regenerant source.
The electrolytic suppressor device also permits recycle of the eluent when installed in a system
with Eluent Recycle (ER) system.
Additionally, an electrolytic suppressor can also act as an electrolytically regenerated neutralizer
for the neutralization of basic or acidic matrices prior to injection. By neutralizing the matrix ions,
trace anionic or cationic species can be detected in strong base or acid samples.
The Thermo Scientific™ Dionex™ Dynamically Regenerated Suppressor (Dionex DRS™ 600
Suppressor) replaces the Thermo Scientific Dionex Electrolytically Regenerated Suppressor
(Dionex ERS™ 500 Suppressor). The Thermo Scientific Dionex Electrolytically Regenerated
Suppressor for External Water (Dionex ERS 500e) and Thermo Scientific Dionex Anion
Electrolytically Regenerated Suppressor for Carbonate Eluents (Dionex AERS 500 Carbonate)
suppressors continue to be supported for their respective applications. The Dionex DRS 600,
Dionex ERS 500e and Dionex AERS 500 Carbonate suppressors are available in 2 mm and 4 mm
formats for use with 2, 3, 4, or 5 mm Ion Chromatography columns and systems. The 2 mm
versions of the Dionex DRS 600, Dionex ERS 500e and Dionex AERS 500 Carbonate are
specially designed with reduced internal volume to ensure optimum performance with 2 and 3
mm columns and systems.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 9 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1.1 Dynamically Regenerated Suppressor
The Dionex DRS 600 is an electrolytic suppressor with a new resin formulation that allows the
suppressor to be operated in the constant voltage mode. The Dionex DRS 600 continues to use
the same clean ion exchange components (screens and membranes) as the Dionex ERS 500
suppressor devices; however, the Dionex DRS 600 eluent channel uses an updated ion exchange
resin composition compared to the Dionex ERS 500 family of suppressors.
The Dionex Dynamically Regenerated Suppressor (Dionex DRS 600) is available in two versions:
the Anion Dynamically Regenerated Suppressor (Dionex ADRS™600) to support anion analysis
applications and the Cation Dynamically Regenerated Suppressor (Dionex CDRS™600) to
support cation analysis applications. The Dionex DRS 600 system consists of a Dynamically
Regenerated Suppressor, the Suppressor Control, back pressure coils, and the Gas Separator
Waste Tube, see Figure 1 The Dynamically Regenerated Suppressor and Accessories. This
high performance, low maintenance AutoSuppression system provides a reliable suppressor
solution for Ion Chromatography.
Additionally, the Dionex DRS 600 offers high capacity suppression while adding minimal delay
volume to the analytical system. The Dionex ADRS 600 provides continuous suppression of
traditional eluents, and more concentrated eluents up to 200 mM NaOH. The Dionex CDRS 600
offers continuous suppression of concentrated eluents up to 100 mN H2SO4or MSA. This high
capacity significantly expands the capabilities and simplifies the operation of Ion
Chromatography.
1.2 DRS 600 Operation
The Dionex DRS 600 is a Dynamically Regenerated electrolytic Suppressor. It is designed to
operate in constant voltage mode in addition to the traditional constant current mode. The unique
formulation of the resin composition in the eluent channel allows for tailoring the current
generated and the device draws the required current for suppressing a given eluent strength. When
a strong eluent enters the eluent chamber electrical current rises, and when a weak eluent enters
the eluent chamber electrical current falls. The net effect is that as long as a constant voltage is
applied the Dionex DRS 600 will self-govern its electrical current.
No need to calculate optimal current –control is a simple on/off control for suppression
up to 100 mM.
Dynamically adjusts the current required for suppression in proportion to the eluent
concentration making it ideal for gradient eluent profiles.
Can accommodate sudden changes in ion load, maintaining suppression efficiency even
with strong ionic strength matrices for example with samples containing high levels of
matrix ions such as groundwater samples.
The Dionex DRS 600 can also be operated in the traditional constant current mode, thus
maintaining compatibility with legacy systems and applications.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 10 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
Figure 1 The Dynamically Regenerated Suppressor and Accessories
Tip
For assistance, contact Technical Support for Dionex Products. In the U.S., call 1-800-346-
6390. Outside the U.S., call the nearest Thermo Fisher Scientific office.
1.3 Suppressor Design
The Dionex DRS 600 and Dionex ERS 500e design comprises of three channels defined by two ion
exchange membranes. The central channel is the eluent channel and the two side channels are
regenerant channels. Two PEEK plates form the outer wall of the regenerant channels and have ¼-28
ports for bringing in the regenerant liquid into and out of the device. The eluent channel is physically
defined by a PEEK plate that seals against the ion exchange membrane and a thin elastomeric O-ring
installed in the regenerant channel. The eluent in and out ports are independent ports that define the
fluidic pathway, similar to a column. The regenerant flow is arranged to be counter-current to the
eluent flow. This orientation ensures complete regeneration of the device. The only difference between
the Dionex DRS 600 and Dionex ERS 500e is the composition of the resin in the eluent channel.
Electrodes are placed along the length of the regenerant channels to completely cover the eluent
channel. In operation, when a DC voltage is applied across the electrodes and the voltage exceeds the
standard potential for the electrolysis of water (approximately 1.5 V), water is electrolytically split to
form electrolysis ions.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 11 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
At the anode H2O 2H+ + 1/2O2+ 2e-
At the cathode H2O + 2e-2OH-+ H2
The electrolysis ions are then available for the suppression reactions. The Dionex DRS 600 suppressor
design allows facile transport of cations or anions depending on which type of suppressor is used for
the application. For example, when pursuing anion analysis with a Dionex ADRS 600, cation exchange
functionality extends across the electrodes. The function of this is to lower the resistance and aid in the
transport of ions in and out of the eluent channel. In the Dionex DRS 600, the eluent channel is filled
with ion exchange resin and provides a static capacity which is particularly useful when eluent is
pumped into the device with the power off.
In operation, the electrolytically generated hydronium ions in the Dionex ADRS 600 are driven towards
the cathode along with eluent cations by the applied voltage. The membrane allows hydronium ions
to pass into the eluent chamber resulting in the conversion of the electrolyte of the eluent to a weakly
ionized form. For each hydronium ion entering the eluent channel one hydronium or a cation exits the
device and is driven towards the cathode. At the cathode, the cations combine with the electrolytically
generated hydroxide ions to form water or base. Overall the current dictates the concentration of
hydronium and hydroxide ions.
The eluent suppression process is illustrated for Anion Suppressor in Figure 2 AutoSuppression
with the Dionex ADRS 600
As shown in Figure 2, the water regenerant undergoes electrolysis to form hydroxide ions on the
cathode surface along with hydrogen gas while hydronium ions are formed in the anode surface along
with oxygen gas. In the Anion Suppressor, cation exchange materials such as screens, membranes, and
resins allow hydronium ions to move from the anode chamber into the eluent chamber to neutralize the
hydroxide eluent. Sodium ions or eluent or sample counter-ions in the eluent are driven by the applied
electric potential towards the cathode and combine with the hydroxide ions generated at the cathode to
form sodium hydroxide waste. Hydronium ions can also travel all the way to the cathode to form water,
thus effecting suppression of the eluent and conversion of the analyte to typically a more conductive
acid form.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 12 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
Figure 2 AutoSuppression with the Dionex ADRS 600
The Dionex DRS 600 (2 mm) suppressor is optimized for MS detection; the suppressor has been
designed to have minimal interference for MS applications and is compatible with operation in
external water mode. The 2-mm suppressor is recommended for applications that use MS
detection due to the improved efficiency, and compatibility with lower operational flow rates
typically employed with MS detection.
The Dionex Anion Electrolytically Regenerated Suppressor for Carbonate Eluents (Dionex AERS
500 Carbonate) is optimized for use with carbonate or carbonate/bicarbonate based eluents and is
available only as an anion version. The Dionex AERS 500 Carbonate hardware design is similar
to the Dionex ADRS 600 hardware design. The Dionex AERS 500 Carbonate uses the same ion
exchange membranes and screens, and resin materials as the legacy Dionex AERS 500
suppressor. The Dionex AERS 500 Carbonate operates similarly to the Dionex ADRS 600
suppressor using electrolysis derived regenerant ions; however, it uses a three-electrode design
for the electrolysis function. The cathode electrode is segmented into two portions with an
electrical gap. These two portions are connected via a resistor thus resulting in a lower applied
current across the outlet section of the suppressor relative to the inlet section of the suppressor.
This design results in decreased gas production at the outlet, and as a consequence, results in less
variation in the suppressed background and is able to achieve low noise.
The eluent suppression process is illustrated for Cation Suppressor in Figure 3
AutoSuppression with the Dionex CDRS 600.
Dionex ADRS 600 in AutoSuppression Mode

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 13 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
As shown in Figure 3, the water regenerant undergoes electrolysis to form hydroxide ions in the
cathode surface along with hydrogen gas while hydronium ions are formed in the anode surface
along with oxygen gas. In the cation suppressor, anion exchange materials such as screens,
membranes, and resins allow hydroxide ions to move from the cathode chamber into the eluent
chamber to neutralize the acid eluent. MSA ions or eluent or sample counter-ions in the eluent
are driven by the applied electric potential towards the anode and combine with the hydronium
ions generated at the anode to form methane sulfonic acid waste. Hydroxide ions can also travel
all the way from the cathode and combine with hydronium ions at the anode to form water, thus
effecting suppression of the eluent and conversion of the analyte to a typically more conductive
base form.
Figure 3 AutoSuppression with the Dionex CDRS 600
CSRS 300 in AutoSuppression Mode
ANODE
+CATHODE -
ANALYTE IN H+ MSA-
ELUENT
(MSA)
Waste/Vent
MSA + O2H2O + H2
Waste/Vent
H2O
H2O
ANALYTE IN H2O
TO DETECTOR
3H2O 2 H2O
2H3O++ ½ O2 (g) H2+ 2 OH-
[H+ + OH-]
H2O
H+ + MSA-
MSA- + H+OH-
Dionex CDRS 600 in AutoSuppression Mode

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 14 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1.4 Overview of Suppression and Neutralization Modes
Four basic modes of suppression can be performed with the Dionex Dynamically Regenerated
Suppressor (Dionex DRS 600) and/or Dionex Electrolytically Regenerated Suppressor (Dionex
ERS 500e and Dionex AERS 500 Carbonate):
AutoSuppression Recycle Mode
AutoSuppression External Water Mode
MPIC Suppression Mode (Dionex DRS 600 and Dionex ERS 500e only)
Neutralization Mode (Dionex DRS 600 and Dionex ERS 500e only)
1.4.1 Mode of Operation Selection
The Dionex DRS 600 and Dionex ERS 500e modes of operation depend mainly on the eluent
composition, the analysis sensitivity requirements and the sample matrix. The compatibilities are
shown in Table 1. For example, eluents containing organic solvents that tend to oxidize easily are
not compatible with the AutoSuppression Recycle Mode. The AutoSuppression External Water
Mode should be used instead, or a Dionex CRS suppressor employed in Chemical Suppression
Mode. The MPIC Suppression Mode is specifically designed for applications where ion-pair
reagents and solvents are present in the eluent. The Neutralization Mode can be used with any
eluent composition.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 15 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
Table 1 Eluent Composition and Suppression Mode Compatibility
Eluent Composition
Recycled Eluent
Mode
External Water
Mode
Chemical
Regeneration Mode(1)
MPIC
Suppression
Aqueous Eluents
(excluding Borate,
Carbonate)
DRS 600
ERS 500e
DRS 600
ERS 500e
DRS 600
ERS 500e
CRS 500
No
Aqueous Borate Eluents
No
DRS 600
ERS 500e
DRS 600
ERS 500e
CRS 500
No
Aqueous Carbonate
Eluents
ADRS 600
AERS 500e
AERS 500 Carbonate
ADRS 600
AERS 500e
AERS 500 Carbonate
ADRS 600
AERS 500e
AERS 500 Carbonate
ACRS 500
No
Aqueous Eluents
containing solvents up to
40%
No
DRS 600
ERS 500e
DRS 600
ERS 500e
CRS 500
DRS 600
ERS 500e
Eluents containing
solvents up to 100%
No
No
DRS 600
ERS 500e
CRS 500
DRS 600
ERS 500e
Eluents containing non-
oxidizable solvents (such
as IPA)
DRS 600
ERS 500e
DRS 600
ERS 500e
DRS 600
ERS 500e
CRS 500
DRS 600
ERS 500e
Eluent containing Ion
Pair Reagents
No
No
No
DRS 600
ERS 500e
Simple Aqueous
Samples
DRS 600
ERS 500e
AERS 500 Carbonate
DRS 600
ERS 500e
AERS 500 Carbonate
DRS 600
ERS 500e
AERS 500 Carbonate
CRS 500
DRS 600
ERS 500e
Complex Aqueous
Samples
No
DRS 600
ERS 500e
AERS 500 Carbonate
DRS 600
ERS 500e
AERS 500 Carbonate
CRS 500
DRS 600
ERS 500e
Samples Containing
Solvents
No
DRS 600
ERS 500e
AERS 500 Carbonate
DRS 600
ERS 500e
AERS 500 Carbonate
CRS 500
DRS 600
ERS 500e
(1)Although the Dionex DRS 600, Dionex ERS 500e and Dionex AERS 500 Carbonate do support Chemical
Suppression Mode, the total suppression capacity is reduced. Use of a Chemically Regenerated Suppressor such as
the CRS 500 or equivalent is recommended.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 16 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1.4.2 The AutoSuppression Recycle Mode
The AutoSuppression Recycle Mode uses the suppressed conductivity cell effluent as the source
of water for the regenerant. This is the preferred method of operation for the Dionex DRS 600
and Dionex AERS 500 Carbonate. The Dionex ERS 500e is also compatible with this mode.
The advantage of this mode of operation is simplicity and ease of use. This mode reliably provides
AutoSuppression for most suppressed conductivity applications using solvent-free eluents. For
solvents that are not easily oxidized, such as isopropyl alcohol, the AutoSuppression recycle mode
is preferred. As the eluent passes through the suppressor’s eluent channel it is converted to a
weakly ionized form. After detection, the cell effluent can be routed back to the regenerant
channel to provide the water required for the electrolysis reactions. The amount of water flowing
through the regenerant chambers is therefore limited to the eluent flow rate. See Section 3 for
complete operating instructions.
The AutoSuppression Recycle Mode is not compatible with eluents containing Borate or
Organic Solvents that tend to oxidize easily, such as methanol or acetonitrile.
1.4.3 The AutoSuppression External Water Mode
The AutoSuppression External Water Mode is used for any application requiring organic solvents
in the eluent or sample, or for applications using borate as the eluent ion. This is the preferred
method of operation for the Dionex ERS 500e. The Dionex DRS 600 and Dionex AERS 500
Carbonate are also compatible with this mode. This mode uses a constant source of deionized
water from a pressurized bottle or another source of deionized water that delivers 0.25 to 2
mL/min for 2 mm applications and 1.0 to 5 mL/min for 4 mm applications, although the
regenerant flow rate is typically recommended as double the eluent flow rate. The amount of water
flowing through the regenerant chambers is independent of the eluent flow rate. The
AutoSuppression External Water Mode eliminates the potential for build-up of contaminating
ions resulting from the oxidation of solvents. It is also recommended when pursuing analysis with
high concentrations of matrix ions, particularly transition metals. Any analysis performed using
the AutoSuppression Recycle Mode can also be performed using the AutoSuppression External
Water Mode. See Section 3 for complete operating instructions.
NOTE
!

1–Introduction
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Product Manual for the DRS 600 Suppressor
Page 17 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1.4.4 The Chemical Suppression Mode
The Dionex DRS 600, Dionex AERS 500 Carbonate and Dionex ERS 500e can be used in the
Chemical Suppression Mode; however, the total suppression capacity is reduced compared to
a Chemically Regenerated Suppressor. Thermo Scientific recommends the use of a Chemically
Regenerated Suppressor such as the Dionex CRS 500 for chemical suppression applications.
1.4.5 The MPIC Suppression Mode
1.4.5.1 Anion MPIC
The Dionex ADRS 600 and Dionex AERS 500e can be used for eluent suppression of Mobile
Phase Ion Chromatography (MPIC or ion-pairing) eluents by using the MPIC Suppression
Mode. The Dionex AERS 500 Carbonate is not recommended for this mode. The MPIC
Suppression Mode is a combination of the AutoSuppression External Water Mode augmented
with a chemical regenerant such as sulfuric acid (H2SO4). The MPIC Suppression Mode uses an
applied current and a constant source of dilute sulfuric acid solution from a pressurized bottle
delivery system. This mode must be used for MPIC applications requiring an ion pair reagent and
organic solvents in the eluent. The MPIC Suppression Mode reliably provides suppression of
typical eluents for MPIC applications using suppressed conductivity detection. The ion pair
reagents, such as tetrabutylammonium hydroxide (TBAOH), are used in concentrations typically
ranging from 1.0 to 5.0 mM. See Section 3 for complete operating instructions.
1.4.5.2 Cation MPIC
The Dionex CDRS 600 and Dionex CERS 500e can be used for eluent suppression of MPIC
eluents by using the AutoSuppression External Water Mode or the MPIC Suppression Mode
depending on the specific MPIC application. The MPIC Suppression Mode uses an applied
current and a constant source of dilute boric acid regenerant solution from a pressurized bottle
delivery system. Dilute boric acid is added to the water regenerant to enhance detection and
improve the linearity of weak bases such as ammonia and amines. This mode is used for MPIC
applications requiring an ion pair reagent and organic solvents in the eluent. The MPIC
Suppression Mode reliably provides suppression of typical eluents for MPIC applications using
suppressed conductivity detection. The ion pair reagents, such as octanesulfonic acid (OSA), are
used in concentrations typically ranging from 1.0 to 5.0 mM. Organic solvent concentrations
should not exceed 40%. See Section 3 for complete operating instructions.
1.4.6 The Neutralization Mode
The Dionex DRS 600 (4 mm), when operated in this mode, requires a constant deionized water
flow of 3 to 5 mL/min through the regenerant chambers. The regenerant water can be delivered
from a pressurized bottle or pump.
IMPORTANT
The Dionex DRS 600 (2 mm), Dionex AERS 500e and Dionex AERS 500 Carbonate
suppressors are not designed or validated for the Neutralization Mode. The Dionex DRS
600 (4mm) suppressor is the only suppressor recommended and validated for this mode.

1–Introduction
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 18 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1.4.6.1 Neutralization Theory
Figure 4 AutoNeutralization of Concentrated Acid/Base with the Dynamically
Regenerated Suppressor
Dionex ADRS 600 Dionex CDRS 600
As shown in Figure 4, when a potential is applied across the two electrodes, the water regenerant
undergoes electrolysis, forming hydronium ions at the anode and hydroxide ions at the cathode.
In the Dionex ADRS 600, hydronium is transported from the anode towards the cathode and
enters the sample chamber. At the same time, the matrix cations in the sample move towards the
cathode and enter the cathode chamber. The matrix cations combine with the hydroxide ions at
the cathode forming a base and are removed from the regenerant chamber. The net effect of this
transport of ions is neutralization of the sample matrix ions to a weakly ionized form while the
sample anions are typically converted to a strongly ionized form. The sample anions are now in a
weakly dissociated matrix such as water and are ready for analysis.
In the Dionex CDRS 600 hydroxide is transported from the cathode towards the anion and enters
the sample chamber. At the same time, the matrix anions in the sample move towards the anode
and enter the anode chamber. The matrix anions combine with the hydronium ions at the anode
forming an acid and are removed from the regenerant chamber. The net effect of this transport of
ions is neutralization of the sample matrix ions to a weakly ionized form, while the sample cations
are typically converted to a strongly ionized form. The sample cations are now in a weakly
dissociated matrix such as water and are ready for analysis
1.4.6.2 Trace Ion Analysis After Neutralization
After neutralization, the sample can be fed into any Dionex Ion Chromatography system and
operated in the concentrator mode. It is important that only low-pressure concentrator columns,
such as the Dionex UTAC-XLP2 (Item # 072781) and Dionex TCC-XLP1 (Item # 063889), be
used to protect the Dionex DRS 600 from damage.

1–Introduction
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Product Manual for the DRS 600 Suppressor
Page 19 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
1.5 Shipment and Storage
1.5.1 Shipment
The Dionex DRS 600, Dionex ERS 500e, and Dionex AERS 500 Carbonate should not be
subjected to temperatures above 50°C for long durations during shipment, storage or
operation, or for any duration above 80°C.
1.5.2 Storage
Ensure the suppressor is stored in a temperature controlled environment away from direct
exposure to sunlight or other sources of heat. Do not store the suppressor in an environment
where temperatures in excess of 50°C may be experienced, such as a parked car.
CAUTION
!
CAUTION
!

2–Installation
Thermo Scientific
Product Manual for the DRS 600 Suppressor
Page 20 of 92
031956-13 For Research Use Only. Not for Use in Diagnostic Procedures.
2. Installation
2.1 System Requirements
The Dionex DRS 600 is designed to be a direct replacement for the Dionex ERS 500 suppressor,
as well as all Dionex SRS series suppressors (Dionex SRS I, Dionex SRS II, Dionex SRS ULTRA,
Dionex SRS ULTRA II and Dionex SRS 300). The Dionex DRS 600 can be used in place of any
of these suppressors where recycled eluent mode or external water mode is employed.
The Dionex ERS 500e is designed for applications where difficult sample matrices or zwitterionic
species are being analyzed. The ion-exchange screen in the Dionex ERS 500e reduces the static
capacity of the suppressor, thus improving compatibility with samples and matrices that have an
affinity for the ion exchange resin found in the Dionex DRS 600.
The Dionex AERS 500 Carbonate suppressor is designed for operation with carbonate and
carbonate/bicarbonate eluents. The Dionex AERS 500 Carbonate features a hardware design that
significantly improves noise performance with carbonate eluents without sacrificing performance
or ease-of-use. The unique hardware design delivers the lowest noise level of any electrolytic
suppressor for carbonate eluents.
The Dionex DRS 600, Dionex ERS 500e and Dionex AERS 500 Carbonate are not designed to
be direct replacements for the Dionex SRS series of suppressors where these suppressors are being
used in Chemical Suppression Mode. If Chemical Suppression Mode is being used, the
Chemically Regenerated Suppressor (Dionex CRS 500) or equivalent is recommended. The
Dionex DRS 600, Dionex ERS 500e and Dionex AERS 500 Carbonate can be used in the
Chemical Suppression Mode; however, the total suppression capacity is reduced compared to a
Chemically Regenerated Suppressor.
The Dionex DRS 600 is also designed to be a direct replacement for the Dionex SRN series of
neutralizers, such as the Dionex SRN 300, Dionex SRN-II and Dionex SRN products.
The Dionex DRS 600, Dionex ERS 500e and Dionex AERS 500 Carbonate are designed to be
run on any Dionex Ion Chromatography System (ICS) equipped with an analytical Anion or
Cation exchange column set and an electrolytic suppressor controller, such as the Thermo
Scientific Dionex ICS-6000, Dionex Integrion, or Dionex Aquion. They are not designed to be
run on a Dionex Capillary Ion Chromatography Systems, such as the ICS-4000, or on Dionex Ion
Chromatography Systems that do not have an electrolytic suppressor controller, such as the
Dionex ICS-90A, Dionex ICS-600 or Dionex ICS-900. Some legacy systems require a
standalone controller for installation of the Dionex ERS 500, Dionex ERS 500e or Dionex
AERS 500 Carbonate. See, “Electrolytically Regenerated Suppressor Requirements for
Selected IC Modules.”
The Dionex DRS and Dionex ERS controls are provided by power supplies that can deliver power
in 1 mA increments up to 500 mA. Discrete (50, 100, 300 and 500 mA) power supplies are
integrated into older systems, such as the CDM-3 and PED-2 of the Dionex DX-300, the Dionex
DX-100 (Model 1-03), Dionex DX-120, Dionex DX-320 (IC20 and IC25 models), Dionex DX-
500 (CD20 and ED40 detectors), and Dionex DX-600 (CD25 and ED50 detectors). The use of
discrete power supplies is no longer supported by Thermo Scientific.