Tianlong GeneRotex 48 User manual

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Automatic Nucleic Acid Extractor
Automatic Nucleic Acid Extractor
User Manual
Created by Xi’an Tianlong Science and Technology Co. Ltd.

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Automatic Nucleic Acid Extractor
Disclaimer and Copyright
Copyright ©(2015-2022) Xi’an TianLong Science and Technology Co., Ltd. (hereinafter
referred to as XATL Co., Ltd.). All rights reserved.
All contents of this manual (including but not limited to text, trademark, logo, buttons,
icons, graphics, tables, data, etc.) are subject to the copyright and other intellectual
property rights of XATL Co., Ltd. These materials shall not be reproduced in any form,
neither mechanical nor electronic, nor translated into any other languages without the
express written permission of XATL Co., Ltd.
Every effort has been made to ensure that all the information contained in this manual is
correct at the time of publishing. However, XATL Co., Ltd. reserves the right to make any
changes necessary. The contents of this manual are subject to change without prior notice
at any time, and the updated revision prevails.
User Manual Document No. : 21EC380000
Version of User Manual: V1.3
User Manual Revision Time: 20- Oct.– 2022
Applicable Model of User Manual: GeneRotex 48
Applicable Software Version Number of User Manual: V1

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Automatic Nucleic Acid Extractor
Trademark Registration Statement:
is the registered trademark of XI’AN TIANLONG SCIENCE
AND TECHNOLOGY CO., LTD.
Intended Use Statement:
The intended use of GeneRotex Automatic Nucleic Acid Extractor (hereinafter referred to
as GeneRotex Extractor) is to complete the automatic extraction and purification of
nucleic acid in various samples by using magnetic bead nucleic acid extraction reagent. The
high purity nucleic acid extraction products are suitable for polymerase chain reaction,
gene chip, gene hybridization, gene sequencing and other technologies, and can be applied
to scientific research, clinical applications, food safety, forensic medicine and other
application fields.
Special Statement:
Before installing and using the GeneRotex Extractor for the first time, please carefully read
the instructions in this manual, fully consider the possible range of misoperation or
non-recommended function, and pay special attention to the possible consequences.
Contraindications:
GeneRotex Extractor is an IVD instrument without contraindications.
User Requirements:
GeneRotex Extractor must be operated by laboratory professionals who have been
trained in laboratory technology and have studied this user manual.

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Automatic Nucleic Acid Extractor
Safety Symbols and Markings
A. Safety Labels on Transport Package
Label
Description
Fragile: the items inside are fragile, please handle with care.
This Side Up: indicates the upward side of the transport package.
Keep Dry: keep the transport package away from rain or any liquid.
IVD Instrument: the product belongs to In Vitro Diagnostic equipment.
Temperature Limit: indicates the temperature limits for the storage and
transportation of package.
Humidity Limit: indicates the humidity limits for the storage and
transportation of package.
Atmospheric Pressure Limit: indicates the atmospheric pressure limits
for the storage and transportation of package.
Max 2 Packages: it is prohibited to stack more than 2 layers for the storage
and transportation of packages.
Recycle: indicates the packaging materials are recyclable.
B. Safety Labels on GeneRotex Extractor
Label
Description
Biohazard: indicates that a certain area of the GeneRotex Extractor can be
easily contaminated with biological reagents or samples. Reminding users to
timely disinfect this area, keep necessary precautions during operation and
take corresponding protective measures at the same time.

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Automatic Nucleic Acid Extractor
High Temp.: indicates that a certain area of the GeneRotex Extractor may
produce high temperature. Reminding users to pay attention and be careful
for burns.
Moving Parts: indicates that a certain moving part of the GeneRotex
Extractor may cause personal injury. Reminding users to proceed with
appropriate caution.
Waste Disposal: indicates that the GeneRotex Extractor belongs to electrical
equipment, the abandoned instrument should not be disposed as unsorted
municipal waste. Please follow local relevant applicable laws and regulations
to deal with the instrument, which must be separately recycled.
The GeneRotex Extractor meets the requirements laid down in Council
Directive 2014/30/EU relating to “Electromagnetic Compatibility” and
Council Directive 2014/35/EU relating to “Low Voltage Equipment”. The
following standards were applied: IEC/EN 61326-1 (EMC), IEC/EN 61010-1
(Safety) and IEC/EN 61010-2-081.
IVD Equipment: The GeneRotex Extractor belongs to In Vitro Diagnostic
equipment.
European Community Representative: indicates the authorized
representative of this instrument in European.
Serial Number: indicates the serial number of this instrument.
Manufacturer: indicates the manufacture of this instrument.
Caution: indicates the “caution” of this instrument.
Manufacture Date: indicates the manufacture date of this instrument.
Consult Instructions for Use: indicates the consult instructions for the use
of this instrument.

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Automatic Nucleic Acid Extractor
C. Symbols Used in This Manual
Symbol
Description
Caution: reminding user pay attention to a certain operation. Operating the
GeneRotex Extractor in any manner unspecified in this manual may results in
instrument damage or abnormal function.
Reminding: providing important information regarding the operation and
successful application of GeneRotex Extractor, including the information
explained in further detail elsewhere in this manual.
Prohibit: prohibiting user from a certain dangerous operation. Otherwise it
may results in instrument damage or abnormal function, even constitutes a
personal injury hazard.
D. Conventions Used in This Manual
Convention
Meaning
Ordered list
Procedure steps must be performed follow the list order.
Press A
Press Akey on the system software.
Bold
Indicates the instructions/options of the system software.
< Bold >
Indicates the keys/icons of the system software.
[ ]
Indicates the keys on computer keyboard.
italic + Blue
Indicates the reference chapter.

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Automatic Nucleic Acid Extractor
Safety and Precautions
The operation, maintenance and repair of GeneRotex Extractor shall strictly follow the
safety specifications listed in this section and through this manual. The design of
GeneRotex Extractor has fully considered its biological contamination protection, electrical
safety protection and mechanical motion protection. Non-observance of the instructions or
performing any operations not stated herein may affect the safety protection provided, and
may also destroy the safety standards of design and manufacture as well as the expected
application scope of the GeneRotex Extractor.
XATL Co., Ltd. will not be responsible for any possible consequence caused by either not read
or violate the instructions mentioned herein.
Caution: please carefully read this manual before operating the GeneRotex Extractor.
Incorrect understanding or operations may result in instrument damage or
inefficiency usage, laboratory damage, even personal injury.
Reminding: please pay attention to the descriptions with ‘Caution’, ‘Reminding’,
‘Prohibit’ symbols, and the safety labels on the instrument and transport package.
I. General Safety and Precautions for Instrument
Caution:
1. Before installing and using GeneRotex Extractor for the first time, please remove the
transport lock in the experiment cabin according to the instructions of user manual
and properly keep the transport lock;
2. Please confirm that the transport lock has been removed before the instrument is
energized;
3. Please reinstall the transport lock before transporting or handling the instrument;
otherwise, the Company will not be responsible for any damage to the instrument.
Otherwise XATL Co., Ltd. will not be responsible for any instrument damage.
Caution: no person except the XATL Co., LTD professional engineers are allowed to
open the instrument, to replace any component or to debug the GeneRotex Extractor.
Caution: do not drop or damage the GeneRotex Extractor, please handle it with care.

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Automatic Nucleic Acid Extractor
Caution: in case of the following situations, immediately cut off the power supply and
contact the dealer or ask the Company’s professional maintenance personnel for
processing.
Any liquid has entered into the GeneRotex Extractor;
Abnormal sound or smell appears while the GeneRotex Extractor is running;
GeneRotex Extractor is soaked with water or rain;
Obvious functional changes of the GeneRotex Extractor.
Prohibit: never handling or move the GeneRotex Extractor while it is running.
Caution: for protection against overheating hazards, the openings on the instrument
are designed for ventilation. Please do not block these openings nor cover the
instrument with dust cover and other materials while it is running.
Caution: the GeneRotex Extractor installation and transportation should be perfomed
by professional engineer or under professional guidelines.
Caution: do not open the experiment cabin forcibly during the operation of the
instrument, otherwise the protective measures of the instrument such as biological
safety and electromagnetic radiation will be damaged.
Caution: do not forcibly place the consumables which are not matched with the
instrument into the experiment cabin.
II. Personal Safety and Precautions
Caution: the GeneRotex Extractor is heavy, please adopt appropriate tools and
methods, or cooperate with other people to complete the lifting or moving of the
instrument. Move or lift the instrument in an improper way may result in bodily
injury, pain, or instrument damage.
Prohibit: never touch the plug, the power cord or the power switch with wet hands.
Prohibit: the GeneRotex Extractor contains permanent strong magnetic field. Anyone
who wears cardiac pacemaker or metal prosthetic device is prohibited to operate the
instrument. The permanent magnet field may affect the functions of the cardiac
pacemaker and metal prosthetic device, even result in damages.
High Temp.: Do not directly touch the heating bars while the instrument is still
running, they may generate enough heat to cause serious burns.

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Automatic Nucleic Acid Extractor
III. Electrical Safety and Precautions
Prohibit: the voltage of GeneRotex Extractor can cause harms to human body, please
cut off the power supply before opening the instrument shell, and it is prohibited to
replace any part of the instrument while it is electrified.
Caution: the GeneRotex Extractor should be properly grounded, any damage of the
internal or external grounding path may cause dangerous.
Caution: in case of electric leakage, immediately unplug the GeneRotex Extractor and
stop using.
Caution: please unplug the power cord before moving the GeneRotex Extractor.
Reminding: under normal circumstances, please use the instrument attached power
cord. If the original power cord is destroyed, please substitute it with an equal one.
Caution: for protection against electric shock hazards, the GeneRotex Extractor must
be grounded properly. The power cord provided is a standard three-pin plug, please
plug it into an appropriate three-wire grounded receptacle for operation safety.
Caution: the power grid environment of GeneRotex Extractor must have ground wire.
Caution: please check the power connection carefully. Hold the power plug when you
plug the power cord and make sure the power plug is perfectly inserted into the
socket, do not pull the power cord to pull out the plug.
Caution: please keep the power cord away from heater or other high temperature
objects. Please do not put anything on the power cord and keep it away from places
where people move around.
Caution: the fuse tube type of the GeneRotex Extractor is F10AH250V, located in the
fuse tube box near the power outlet at the rear of the instrument. Use improper fuse
tube may lead to circuit system damage. Please check and make sure that the fuse
tube has been properly installed before switch on the instrument.
Caution: before replacing the fuse tube, please cut off the instrument power supply,
unplug the power plug and use a screwdriver to pry open the fuse box. Then
substitute the old fuse tube with an equal one.

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Automatic Nucleic Acid Extractor
IV. Environmental Safety and Precautions
Caution: the GeneRotex Extractor is for indoor use only, the room should be well
ventilated and without corrosive gas.
Prohibit: never run the GeneRotex Extractor in places that have or may have
flammable and explosive gas.
Prohibit: spraying liquid on electrical parts may cause a short circuit and result in fire,
do not use sprays in vicinity of the GeneRotex Extractor.
Reminding: the working environment temperature of GeneRotex Extractor should be
between 10°C~30°C, and the relative humidity should be below 80%.
Reminding: the working environment of the GeneRotex Extractor should be under
normal atmosphere (The altitude should be below 4000m).
V. Biological Safety and Precautions
Biohazard: the sample objects of the GeneRotex Extractor are nucleic acids, please
consider all samples are with potential biohazard, and please take applicable safety
protection measures and wear appropriate protective goggles, clothing and gloves
while processing the samples.
Biohazard: in case of any liquid overflows during the operation, please immediately
disinfect the contaminated area with appropriate detergent to avoid the spreading of
contaminant, prevent the laboratory or instrument from biohazards.
Biohazard: please comply with the local or national applicable regulations to
complete the disposal of waste samples and contaminated materials.
Biohazard: users should consider the abandoned GeneRotex Extractor as biological
contaminated material and comply with the local or national applicable regulations to
complete the instrument disposal. Before disposal or recycle the instrument, please
completely clean and disinfect the instrument.
Biohazard: users should only use reagents and consumables that are within their
expiration date.

Automatic Nucleic Acid Extractor
Contents
1. INTRODUCTION................................................................................................................... 1
1.1 INTENDED APPLICATION FIELDS .................................................................................................................... 1
1.2 OPERATING PRINCIPLE .....................................................................................................................................2
1.3 INSTRUMENT APPEARANCE AND COMPOSITION ..........................................................................................3
1.3.1 Instrument Structure.................................................................................................................................3
1.3.2 Experiment Cabin Structure...................................................................................................................4
1.4 PRODUCT INTRODUCTION ................................................................................................................................5
1.4.1 General Parameters of Instrument..................................................................................................... 5
1.4.2 Performance Parameters of Instrument...........................................................................................6
1.4.3 Instrument Characteristics.....................................................................................................................6
1.4.4 Software System Characteristics..........................................................................................................7
1.4.5 Reagent Characteristics............................................................................................................................8
1.4.6 Consumable Specification........................................................................................................................8
2. UNPACKING AND INSTALLATION................................................................................... 9
2.1 UNPACKING INSTRUCTIONS ..............................................................................................................................9
2.1.1 Unpacking Steps........................................................................................................................................ 10
2.2 WORKING ENVIRONMENTAL REQUIREMENTS ...........................................................................................12
2.2.1 Space Requirements of Instrument.................................................................................................. 12
2.2.2 Power Requirements of Instrument................................................................................................. 12
2.3 INSTRUMENT INSTALLATION .........................................................................................................................14
2.3.1 Remove Transport Lock ......................................................................................................................... 14
2.3.2 Instrument Self Inspection................................................................................................................... 15
3. INSTRUMENT OPERATION INSTRUCTIONS ............................................................. 17

Automatic Nucleic Acid Extractor
3.1 INSTRUMENT START-UP .................................................................................................................................17
3.2 SYSTEM SOFTWARE OPERATION .................................................................................................................. 17
3.2.1 Main Interface............................................................................................................................................17
3.2.2 Experiment File Tab.................................................................................................................................20
3.2.3 Run Monitoring Tab.................................................................................................................................26
3.2.4 General Setting Tab................................................................................................................................. 29
4. INSTRUMENT CLEANING AND MAINTENANCE....................................................... 34
4.1 INSTRUMENT CLEANING OPERATION INSTRUCTIONS ...............................................................................34
4.1.1 Clean Instrument Shell...........................................................................................................................35
4.1.2 Clean Operation Screen..........................................................................................................................35
4.1.3 Clean Experiment Cabin........................................................................................................................ 35
4.2 INSTRUMENT MAINTENANCE OPERATION INSTRUCTIONS ......................................................................36
4.2.1 Maintain Sufficient Air Flow................................................................................................................36
4.2.2 Maintain Stable Power Supply............................................................................................................36
4.2.3 Maintain Instrument Cleanliness...................................................................................................... 36
4.2.4 Other Maintenance...................................................................................................................................37
4.3 TRANSPORT AND RETURN FOR MAINTENANCE ........................................................................................ 37
4.3.1 Instrument Disinfection.........................................................................................................................37
4.3.2 UV Disinfection...........................................................................................................................................38
4.3.3 Installation of Transport Locks..........................................................................................................39
4.3.4 Product Packaging and Return for Maintenance......................................................................41
5. TROUBLESHOOTING........................................................................................................42
6. EXPERIMENT OPERATION GUIDE............................................................................... 44
6.1 OVERVIEW OF EXPERIMENT OPERATION GUIDE .......................................................................................44
6.2 EXPERIMENT PREPARATION ......................................................................................................................... 44
6.2.1 Scan Reagent Kit Code............................................................................................................................44

Automatic Nucleic Acid Extractor
6.2.2 Reagent and Sample Preparation.....................................................................................................45
6.3 LOADING OF DEEP WELL PLATE ..................................................................................................................45
6.4 EDIT AND RUN EXPERIMENT PROGRAM .................................................................................................... 46
6.4.1 Edit Experiment Program.....................................................................................................................46
6.5 SHUTDOWN ......................................................................................................................................................47
6.6 MATTERS FOR ATTENTION ............................................................................................................................47
7. CONTACT INFORMATION................................................................................................49

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Automatic Nucleic Acid Extractor
1. Introduction
1.1 Intended Application Fields
The GeneRotex Extractor can be widely used in scientific research, clinical diagnosis,
disease control, food safety, forensic medicine and other fields. The user only needs to add
samples and magnetic bead nucleic acid extraction reagents and load reaction consumables,
and then the instrument will complete all nucleic acid extraction operations according to
the experimental program and rapidly extract DNA and RNA from animal and plant tissues,
blood, body fluids, criminal materials and other samples by using different magnetic bead
nucleic acid extraction reagents.
Figure 1-1. GeneRotex Automatic Nucleic Acid Extractor
The GeneRotex Extractor is equipped with the following status indicator lamps:
Heating: indicates GeneRotex Extractor is running the heating step;
Stirring: indicates GeneRotex Extractor is running the stirring g step;
Magnetic: indicates GeneRotex Extractor is running the magnetic absorption step;
Wait: indicates GeneRotex Extractor is running the waiting step;

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Automatic Nucleic Acid Extractor
1.2 Operating Principle
The GeneRotex Extractor is designed as a high-tech product to extract the nucleic acid
through magnetic beads method. It is characterized as high degree of automation, fast
extraction speed, reliable results and easy operation. By utilizing of 48 deep well plates, it
is capable of simultaneously deal with 1 to 48 samples.
The GeneRotex Extractor takes advantage of the magnetic rods that located on magnetic
rod frame, automatically transfer the magnetic beads-nucleic acid mixture from one well to
another, then through the movement of magnetic rods and mixing sleeves, the instrument
quickly and repeatedly stirring the reagent, evenly mix the reagent with the magnetic
beads. Through the cell lysis, nucleic acid adsorption, washing and elution processes, the
instrument will complete the extraction of nucleic acids and obtain highly purified nucleic
acids, as shown in the figure 1-2.
Figure 1-2. Principle steps of magnetic beads processing

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Automatic Nucleic Acid Extractor
1.3 Instrument Appearance and Composition
1.3.1 Instrument Structure
Figure 1-3. GeneRotex Automatic Nucleic Acid Extractor - Front View
Figure 1-4. GeneRotex Automatic Nucleic Acid Extractor - Rear View

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Automatic Nucleic Acid Extractor
1.3.2 Experiment Cabin Structure
Figure 1-5. GeneRotex Extractor - Experiment cabin
1. Operation screen
2. Experiment cabin lifting window
3. Experiment cabin overhead door
4. Heat dissipation opening
5. Power switch
6. Fuse tube holder
7. Power socket
8. UV lamp replacement door
9&10. USB interface
11. Heating bar
12. Deep-well plate positioning strip
13. Experiment cabin movable loading platform

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Automatic Nucleic Acid Extractor
1.4 Product Introduction
1.4.1 General Parameters of Instrument
▶Instrument Specification:
Dimension:490mm(L) × 510mm (W) × 480mm (H);
Weight: 45kg;
▶Package Specification:
Dimension: 700mm(L) × 660mm (W) × 580mm (H);
Weight: 55kg;
▶Power Specification:
Voltage: AC 100V-240V;
Frequency: 50/60Hz;
Rated power: 600VA;
▶Communication Specification:
Network port: TCP/IP protocol;
Ethernet connection;
USB interface;
▶Application Environment:
Temperature: 10°C ~30°C;
Relative humidity: ≤80%RH, non-condensing;
Atmospheric pressure: 56.0kPa~106.0kPa;
Altitude: below 4000m;
▶Storage and Transportation Environment:
Temperature: -40°C~55°C, with transport package;
Relative humidity: 0~93% RH;
Atmosphere pressure: 26.4kPa~106.0kPa.
▶Operating Noise:
The maximum operating noise is lower than 80 decibles.

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Automatic Nucleic Acid Extractor
1.4.2 Performance Parameters of Instrument
▶Processing Volume:
Well 1 is 50μL to 3000μL; Well 2-5 is 50μL to 1500μL; Well 6 is 30μL to 1000μL.
▶Sample Flux:
1-48 fluxes;
▶Inter-well Extraction Difference:
CV <3% (For identical concentration samples that are extracted in identical process).
▶Heating Temperature:
The heating module can be optionally configured to realize lysis heating (room
temperature to 120℃) and elution heating (room temperature to 120℃);
▶Temperature Control:
Average Heating Rate: ≥0.5℃/s;
Temperature Uniformity: ≤3℃;
Temperature Accuracy: ±1℃;
▶Rotational Speed: 100-3000rpm adjustable.
1.4.3 Instrument Characteristics
▶Screen Operation:
The GeneRotex Extractor is embedded with a 7-inch color operation screen, which is
capable of independently operating and monitoring real-time running status.
▶Precise Control:
Built-in engineering computer, which can be operated alone without connection to a
computer.
▶Temperature Control:
User can customize the lysis and elution temperatures on demand.