Tianlong Real-time PCR System User manual

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Real-Time PCR System
Real-time PCR System
User Manual
Xi’an Tianlong Science and Technology Co., LTD.
Bring Technology to Life

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Real-Time PCR System
Disclaimer and Copyright
Copyright © 2019 Xi’an Tianlong Science and Technology Co., Ltd. (hereinafter
referred to as XATL Co., Ltd.). All rights reserved.
All contents of this manual (including but not limited to text, trademark, logo, buttons
icons, graphics, tables, data, etc.) are subject to the copyright and other intellectual
property rights of XATL Co., Ltd. These materials shall not be reproduced in any form,
neither mechanical nor electronic, nor translated into any other languages without the
express written permission of XATL Co., Ltd.
Every effort has been made to ensure that all the information contained in this manual
is correct at the time of publishing. However, XATL Co., Ltd. reserves the right to make
any changes necessary. The contents of this manual are subject to change without prior
notice at any time, and the updated revision prevails.
User Manual Version:V1.0
User Manual Revision Time: 6-April–2021
Suitable Instrument Model:Gentier Mini

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Real-Time PCR System
is a registered trademark for Xi’an Tianlong
Science and Technology corporate limited.
Intended Use
The Gentier mini real-time PCR system (hereinafter referred to as Gentier mini
instrument) is intended for performing rapid, accurate polymerase chain reaction
(PCR), meanwhile real-time measuring nucleic acid signals from DNA-binding
fluorescent dyes or labeled probes and converts them to comparative quantitative
readouts of DNA or reverse transcribed RNA.
Special Declaration
Before installing and operating the Gentier mini instrument, please read this manual
carefully, observe the warnings and non-recommended functions. Please also bear mind
the potential scope for misuse; it is advisable to draw attention to the possible
consequences.
User Requirements
The Gentier mini instrument must only be used by laboratory professionals who have
been trained in laboratory techniques and read this manual carefully.

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Real-Time PCR System
Safety Labels on Transport Package
Label
Description
Fragile: The items inside are fragile, please handle with care.
This Side Up: indicates the upward side of the transport package.
Keep Dry: keep the transport package away from rain or any liquid.
IVD Instrument: The product belongs to In Vitro Diagnostic equipment.
Temperature Limit: indicates the temperature limits for the storage and
transportation of package.
Humidity Limit: indicates the humidity limits for the storage and
transportation of package.
Atmospheric Pressure Limit: indicates the atmospheric pressure limits
for the storage and transportation of package.
Max 6 Packages: It is prohibited to stack more than 6 layers for the
storage and transportation of packages.
Recycle: indicates the packaging materials are recyclable.
Safety Labels on Instrument
Label
Description
Biohazard: indicates that a certain area of Gentier mini instrument can be
easily contaminated with biological reagents or samples. Reminding users
to timely disinfect this area, keep necessary precautions during operation
and take corresponding protective measures at the same time.
High Temp.: indicates that a certain area of the Gentier mini instrument
may produce high temperature. Reminding users to pay attention and be
careful for burns.

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Real-Time PCR System
Moving Part: indicates that a certain moving part of Gentier mini
instrument may cause personal injury. Reminding users to proceed with
appropriate caution.
IVD Equipment: Gentier mini instrument belongs to In Vitro Diagnostic
equipment.
Symbols Used in This Manual
Symbol
Description
Caution: reminds users to pay attention to a certain operation.
Operating the Gentier mini instrument in any manner unspecified in this
manual may result in instrument damage or abnormal function.
Reminding:provides important information regarding the Gentier mini
instrument operation and successful application, including the
information explained in further details elsewhere in this manual.
Prohibit: prohibits users from a certain dangerous operation.
Otherwise, it may result in instrument damage or abnormal functions,
and even constitute a personal injury hazard.
Conventions Used in This Manual
Convention
Meaning
Ordered list
Procedure steps must be performed following the list order.
(Double) Click A
Click A on the application software.
Click A > B
Click B in menu Aon the application software.
Italic + Bold
Refers to the instructions/options of the application software.
< Italic + Bold >
Refers to the keys/icons of the application software.

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Real-Time PCR System
Bold
Refers to the instructions/options of the instrument system
software.
< Bold >
Refers to the keys/icons of the instrument system software.
[ ]
Refers to the keys on computer keyboard.
italic + Blue
Indicates the reference chapter.

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Real-Time PCR System
Safety and Regulatory Compliance
The operation, maintenance and repair of Gentier mini instrument shall strictly follow
the safety specifications listed in this section and through this manual. The design of
Gentier mini instrument has fully considered its biological contamination protection,
electrical safety protection and mechanical motion protection. Non-observance of the
instructions or performing any operations not stated herein may affect the safety
protection provided, and may also destroy the safety standards of design and
manufacture as well as the expected application scope of the Gentier real-time PCR
system.
XATL Co., Ltd. will not be responsible for any possible consequence caused by either not
read or violate the instructions mentioned herein.
Caution: Please carefully read this manual before operating Gentier Mini
instrument. Incorrect understanding or operations may result in instrument
damage or inefficiency usage, laboratory damage, even personal injury.
Reminding:Please pay attention to the descriptions with “Caution”, “Reminding”,
“Prohibit”symbols, and the safety labels on the instrument and transport package.
I. General Instrument Safeties and Precautions
Caution: No person except the XATL Co., LTD professional engineers are allowed
to open the instrument, to replace any component or to debug Gentier Mini
instrument.
Caution: Do not drop or damage the instrument, and please handle it with care.
Caution: In case of any following conditions, immediately cut off the power supply
and contact the distributor or manufacturer to ask for professional engineer for
processing.
Any liquid has entered into the instrument;
Abnormal sound or smell appears while the instrument is running;
The instrument is soaked with water or rain;
Obvious functional changes of the instrument.

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Real-Time PCR System
Prohibit: Never handling or move the instrument while it is running.
Caution: For protection against overheating hazards, the openings on the
instrument are designed for ventilation. Please do not block these openings or
cover Gentier Mini instrument with dust cover and other materials while it is
running.
Caution: Do not open the top lid while the instrument is running. This may break
the biological safety and electromagnetic radiation protection measures of the
instrument.
Caution: Do not force to place unmatched consumables into the sample block.
Caution: Users can move the instrument when it is power off but should observe
the following principles:
The instrument is power off.
All network and power cords are detached from Gentier Mini instrument.
Gentier Mini instrument should be packed properly if there is a long-distance
movement. See G-3 Transportation or Return to Factory for more details.
II. Personal Safeties and Precautions
Caution: Please move the instrument according to the requirements of the user
manual. Moving the instrument in an improper way may result in body injury, pain
or instrument damage.
Prohibit: Never touch the plug, the power cord or the power switch with wet hands.
High Temp.: Do not directly touch the sample block and the hot lid while the
instrument is running. They may generate enough heat to cause serious burns.
Please wait the sample block to return to idle temperature before opening the top
lid.

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Real-Time PCR System
III. Electrical Safeties and Precautions
Prohibit: Please cut off the power supply before opening the instrument shell, and
it is prohibited to replace any part of the instrument while it is electrified.
Caution: The instrument should be properly grounded. Any damage of the internal
or external grounding path may cause danger.
Caution: Once the electric leakage is found, please cut off the power supply
immediately and stop using the instrument.
Caution: Please unplug the power cord before moving the instrument.
Reminding: Under normal circumstances, please use the attached power cord of
the instrument. If the original power cord is destroyed, please substitute it with an
equal one.
Caution: The ground wire is necessary in the power grid environment.
Caution: The instrument must be grounded properly for protection against electric
shock hazards. The instrument adapts with a standard three-pin plug, and please
plug it into an appropriate three-wire grounded receptacle for operation safety.
Caution: Please check the power connection carefully. Hold the power plug when
you plug the power cord and make sure the power plug is perfectly inserted into
the socket, do not pull the power cord to pull out the plug.
Caution: Please keep the power cord away from heater or other high temperature
objects. Please do not put anything on the power cord and keep it away from places
where people move around.
IV. Environmental Safeties and Precautions
Caution: The instrument is for indoor use only. The room should be well ventilated
and without corrosive gas.
Prohibit: Never run the instrument in places that have or may have flammable and
explosive gas.

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Real-Time PCR System
Prohibit: Spraying liquid on electrical parts may cause a short circuit and result in
fire, so do not use sprays in vicinity of the instrument.
Reminding: The working environment temperature of the instrument should be
between 15°C ~30°C , and the relative humidity should be between 20%~85%.
Reminding:The working ambient pressure of the instrument should be
50kPa~106kPa (the altitude should below 4000m).
V. Biological Safety and Precautions
Biohazard:The sample objects of the instrument are nucleic acids and please
consider all samples as those with potential biohazard. Please take applicable safety
protection measures and wear appropriate protective goggles, clothing and gloves
while processing the samples.
Biohazard: In case of any liquid overflows during the operation, please
immediately disinfect the contaminated area with appropriate detergent to avoid
the spreading of contaminant.
Biohazard:Please comply with the local or national applicable regulations to
complete the disposal of waste samples and contaminated materials.
Biohazard:Users should consider the abandoned Gentier mini instrument as
biological contaminated materials and comply with the local or national applicable
regulations to complete the instrument disposal. Before the disposal or recycling of
the instrument, please completely clean and disinfect the instrument.
Biohazard:Users should only use reagents and consumables within their
expiration dates.

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Contents
A. OVERVIEW.....................................................................................................................................1
A-1. APPLICATION FIELDS.................................................................................................................................1
A-2. PRODUCT CONSTITUTE AND INSTRUMENT STRUCTURE......................................................................2
A-3. PRODUCT INTRODUCTION.........................................................................................................................2
A-3.1 Instrument Parameters and Characters ............................................................................2
A-3.1.4 Running Mode Difference......................................................................................................6
A-3.2 Software Characters ...................................................................................................................7
A-3.3 Reagent Specifications...............................................................................................................7
A-3.4 Consumable Specifications.......................................................................................................8
B. INSTRUMENT UNPACKING AND INSTALLATION.............................................................9
B-1. UNPACKING INSTRUCTIONS ......................................................................................................................9
B-1.1 Unpacking Steps ........................................................................................................................ 10
B-2. WORKING ENVIRONMENTAL REQUIREMENTS ................................................................................... 11
B-2.1 Instrument Space Requirements ........................................................................................ 11
B-2.2 Instrument Power Requirements ....................................................................................... 12
B-3. INSTRUMENT INSTALLATION ................................................................................................................13
B-3.1 External Device Connection.................................................................................................. 13
B-3.2 Application Software Installation...................................................................................... 14
B-3.3 Computer Network Setting................................................................................................... 15
C. PREPARATION BEFORE EXPERIMENT ............................................................................. 17
C-1. INSTRUMENT SELF-INSPECTION ...........................................................................................................17
C-2. SAMPLE LOADING....................................................................................................................................17

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Real-Time PCR System
D. GENERAL MODE - INSTRUMENT SOFTWARE OPERATION....................................... 19
D-1. STARTUP INTERFACE &MAIN INTERFACE......................................................................................... 19
D-2. NEW EXPERIMENT .................................................................................................................................22
D-2.1 Experiment Settings................................................................................................................. 22
D-2.2 Run Monitoring.......................................................................................................................... 26
D-3. GENERAL SETTINGS................................................................................................................................ 28
D-3.1 Settings Tab................................................................................................................................. 29
D-3.2 Network Tab ............................................................................................................................... 30
D-3.3 Projects Tab ................................................................................................................................ 32
D-3.4 About Tab..................................................................................................................................... 34
D-4. EXPERIMENTAL DATA............................................................................................................................ 36
D-4.1 Experimental Data Interface............................................................................................... 36
D-4.2 Result Analysis ........................................................................................................................... 37
E. ADVANCED MODE - APPLICATION SOFTWARE OPERATION ................................... 42
E-1. START APPLICATION SOFTWARE.......................................................................................................... 42
E-2. QUICK START BAR .................................................................................................................................. 43
E-2.1 User Management..................................................................................................................... 44
E-2.2 Quick Start ................................................................................................................................... 45
E-2.3 Recent Files.................................................................................................................................. 47
E-2.4 Other Functions of Quick Start............................................................................................ 48
E-3. MAIN INTERFACE .................................................................................................................................... 49
E-3.1 Menu Bar....................................................................................................................................... 49
E-3.2 Tool Bar......................................................................................................................................... 70
E-3.3 Operation Area........................................................................................................................... 72

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F. ADVANCED MODE - INSTRUMENT SOFTWARE OPERATION..................................114
F-1. MAIN INTERFACE ................................................................................................................................. 114
F-2. INSTRUMENT SOFTWARE OPERATION AREA................................................................................... 116
F-2.1 Experiment File ........................................................................................................................117
F-2.2 Run Setting.................................................................................................................................120
F-2.3 Run Monitoring ........................................................................................................................128
F-2.4 Result Analysis..........................................................................................................................132
F-2.5 General Setting.........................................................................................................................134
G. INSTRUMENT CLEANING AND MAINTENANCE............................................................140
G-1. INSTRUMENT CLEANING OPERATION INSTRUCTIONS .................................................................... 140
G-1.1 Clean Instrument Shell..........................................................................................................140
G-1.2 Clean Touch Screen ................................................................................................................141
G-1.3 Clean Sample Block ................................................................................................................141
G-2. INSTRUMENT MAINTENANCE OPERATION INSTRUCTIONS ........................................................... 142
G-2.1 Maintain Sufficient Air Flow ..............................................................................................142
G-2.2 Maintain Stable Power Supply. .........................................................................................142
G-2.3 Maintain Instrument Cleanliness .....................................................................................143
G-3. TRANSPORTATION OR RETURN TO FACTORY .................................................................................. 143
G-3.1 Instrument Disinfection........................................................................................................143
G-3.2 Product Packaging .................................................................................................................144
G-4. TROUBLESHOOTING ............................................................................................................................. 146
H. AFTER-SALE SERVICE COMMITMENTS..........................................................................148
H-1. WARRANTY SERVICE........................................................................................................................... 148
H-2. RESPONSE TIME................................................................................................................................... 149
H-3. CONTACT INFORMATION .................................................................................................................... 149
APPENDIX 1 - PRODUCT ACCESSORIES LIST ....................................................................150

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Real-Time PCR System
A. Overview
A-1. Application Fields
Gentier mini real-time PCR system is intended for performing rapid and accurate
polymerase chain reaction (PCR). It can be used to detect target nucleic acids in
biological samples, including nucleic acids of various pathogens (viruses, bacteria,
mycoplasma, chlamydia, etc.) and genes. Gentier mini real-time PCR system can be
applied to the fields of animal and plant medical treatment, disease control, inspection
and quarantine, etc.
1. Top lid
2. Touch screen
3. USB interface
4. Side air vent
5. TCP/IP network interface
6. Front air vent
7. Release button of top lid
8. Lock catch of top lid
9. Hot lid
10. Heat insulating washer
11. Sample block
12. Power switch
13. Instrument handle
14. Electrical connection line
15. DC power input interface
16. Rear air vent
17. Nameplate
Figure A-1. Instrument Structure

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Real-Time PCR System
A-2. Product Constitute and Instrument Structure
Gentier mini real-time PCR system is mainly consisted of control system, power system,
temperature control system, detection system, man-machine interface system and
software (Version: V1), etc.
A-3. Product Introduction
A-3.1 Instrument Parameters and Characters
A-3.1.1 Instrument General Parameters
►Instrument Specification:
Dimension: 205mm(L) x 156mm (W) x 153mm(H);
Weight: 3.2kg;
►Package Specification:
Dimension: 275mm(L) ×190mm (W) ×230mm (H);
Weight: 4.5kg;
►Power Specification:
DC24V 6.25A, 150W;
(The instrument is equipped with a special AC power adapter.)
►Power Adapter:
Input: AC 100-240V, 2.5A, 50~60Hz;
Output: DC24V 6.25A, 150W;
►Communication Specification:
Network port: TCP/IP protocol;
Ethernet connection;
USB 2.0, WiFi (IEEE 802.11 b/g/n);

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►Application Environment:
Temperature: 15°C ~30°C ;
Relative humidity: 20%~85%, non-condensing;
Atmospheric pressure: 50.0kPa~106.0 kPa;
Altitude: below 4000m;
►Storage and Transportation Environment:
Temperature: -20°C ~55°C , with transport package;
Relative humidity: 0%~ 93%;
►Running Noise:
The maximum running noise does not exceed 65 decibels.
A-3.1.2 Instrument Technical Parameters
A-3.1.2.1 Thermal Parameters
►Temperature Accuracy: ≤0.1°C ;
►Temperature Uniformity: ±0.2°C ;
►Temperature Precision: ≤0.1°C ;
►Max Heating Ramp:5.0°C/s;
►Max Cooling Ramp:4.0°C/s.
A-3.1.2.2 Optical Parameters
Excitation Light Source: LED light sources;
Detection System: photodiodes;
Optical Channel & Dye:
Channel 1: FAM, SYBR Green I, etc.
Channel 2: VIC, HEX, TET, JOE, etc.

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A-3.1.2.3 Detecting Parameters
►Throughputs: simultaneously detect 16 samples;
►Repeatability: CV ≤0.5%;
►Linear Correlation:│r│≥0.990, within linear range of test items.
A-3.1.2.4 Man-machine Interactive System Parameters
►Touch Screen: with built-in 7.0’inches full-color touch screen. Gentier Mini
instrument can run alone without the main control computer.
►USB interface: The USB interface of this instrument is only used to connect USB
disk and USB printer.
Prohibit: The USB interface cannot be used for charging electronic devices.
Prohibit: Do not connect with external devices when the instrument is running
an experiment.
Reminding: USB devices connected with Gentier Mini instrument need to pass
the safety certification to ensure that the instrument will not be damaged.
Reminding:Before connecting the USB disk to Gentier Mini instrument, please
format the USB disk to FAT32 format and confirm that there is enough space left
in the USB disk. It is recommended to reserve 1GB.
►Main Control Computer: In advanced mode, Gentier Mini instrument can be
connected to the main control computer for remote control. The recommended
configurations of main control computer are listed as below. Customers are
allowed to purchase by themselves.
CPU:Quad-core CPU (or higher configurations)
Internal Storage: 8G (or higher configurations)
Hard Disk: 500GB (or higher configurations)

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►Operating System:applicable to Windows 7 64bit/32bit, Windows 8
64bit/32bit and Windows 10 64bit/32bit.
►Application: Excel 2007(or higher configurations)
►Network Control: One computer can be connected with multiple Gentier mini
instruments to realize the network centralized control and remote control.
Reminding: When Gentier Mini instrument runs in general mode, it does not
need to be connected to the main control computer.
A-3.1.3 Instrument Characters
►Flexible and Convenient: Two running modes - general mode and advanced
mode, are provided to users for convenience. Users can obtain detection results
in general mode and realize remote control in advanced mode.
►Running alone: Gentier Mini instrument can run independently without
connection to the main control computer in both modes.
►Independent Temperature Control: Gentier Mini instrument adopts
independent temperature control technology and high repeatability
temperature zone to realize the optimization of PCR.
►Power-off Protection: Gentier Mini instrument possesses protection function
against instantaneous power off, which can continue to run the uncompleted
experiment after restarting the instrument.
►Multiple PCR Step Modes: Gentier Mini instrument provides multiple PCR step
modes, such as standard step, touchdown step and long step.
►Remote Running: Gentier Mini instrument can be connected to the main control
computer in advanced mode to realize remote control, such as test editing,
sample editing, experiment running, run monitoring and data transmission.

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A-3.1.4 Running Mode Difference
Function Description
General
Mode
Advanced
Mode
Remarks
Instrument Program Selection
√
√
Instrument Program Editing
Ⅹ
√
Instrument Sample
Application
√
√
Detailed Sample Editing
Ⅹ
√
In General mode, users can set
sample type and sample
number, but cannot set target
gene, repeated group, standard
and concentration.
Running Alone
√
√
Instrument Test Result Output
√
√
Output simple analysis results,
such as PCR Curve, CT Value.
Instrument Test Report
Printing
√
√
External USB printer is
required.
Remote Control
Ⅹ
√
Result Analysis
Ⅹ
√
Analyze experimental results in
detail, and set analysis
parameters and create new
analyzing modes, etc.
Report System
Ⅹ
√
Report system can only be used
on the application software on
the main control computer.
Data Transmission
Ⅹ
√
In advanced mode, data
transmission can be realized
when the instrument is
controlled remotely by the main
control computer.