UGO BASILE 7025 Series User manual
UGO BASILE S.R.L.
ugobasile
.com
instruction manual
Rodent Ventilator
Cat. No. 7025 / 7125
UGO BASILE S.R.L.
Via G. Di Vittorio, 2
21036 GEMONIO, VA, ITALY
Phone: +39 0332 744574
instruction manual
Rodent Ventilator
Cat. No. 7025 / 7125
www.
ugobasile
.com
Serial No.
SAFETY CONSIDERATIONS
ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL SAFE-
TY STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND WARN-
INGS WHICH MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO RETAIN
THE INSTRUMENT IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSON-
NEL, AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UN-
DER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITA-
BLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE
HAZARD INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE IN-
STRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated October 2013
Revision 2
www.ugobasile.com
“We have four of your respirators in our extended
lab and they are wonderful - as is your service”
Dr. Nicholas Price, Monash University
Rodent Ventilator
General
The 7025 Rodent Ventilator is a volume-con-
trolled mechanical ventilator (according to Star-
ling’s ventilator method), designed for use
with rats, guinea pigs, mice and small birds.
The 7025 drive consists of a variable speed geared
motor linked by a novel variable stroke mechanism
to easily interchangeable cylinder/piston assem-
blies.
In particular, the 7025 can be equipped with 5, 10
or 30ml cylinder/piston assembly.
Its precisely regulated geared-motor speed provi-
des the most accurate and reliable stroke rate con-
trol of any respirator available.
The operation of the 7025 may be “paused” by an
external TTL logic signal.
The picture features a Rodent Ventilator 7025, together
with the 6025 for Cat/Rabbit
VENTILATORS and GAS ANESTHESIA
Cat. No. 7025
Best available
Starling
Pumps
THE CHOICE OF
THE CRITICS!
Main Features
l Interchangeable cylinder/piston assemblies (5, 10, 30ml)
l Quiet operation, both acustically and electrically (negligible R.F. broadcasting)
l Reliable mechanics and impeccable nishing: lifelong lasting
l Synchronised START/STOP function available as optional
Ugo Basile: more than 10,000 citations
7025
6025
CHECK-LIST
7025
Rodent Ventilator, 10 ml
7025-30
Rodent Ventilator, 30 ml
7025-5
Rodent Ventilator, 5 ml
7025-1
Rodent Ventilator, 5 ml
7125
Rodent Ventilator, Start/Stop, 10 ml
7125-30
Rodent Ventilator, Start/Stop, 30 ml
7125-5
Rodent Ventilator, Start/Stop, 5 ml
7125-1
Rodent Ventilator, Start/Stop, 1 ml
CLIENTE / CUSTOMER______________________________________________
Ordine No. / Order No. ____________________ Data / Date____/_____/______
UB code
CAT.No.
Q.ty DESCRIPTION DESCRIZIONE
E-WP 008 1 MAINS CABLE EUROPE CAVO RETE EUROPA
E-WP 008-1 U.S.A. U.S.A.
E-FT 008-1 2 FUSES (T800mA) FUSIBILI (T800mA)
7044 1 Y-CANULA CANULA a Y
M-CM 081-F 7033 1 LITHIUM-GREASE GRASSO AL LITIO
7032 1 PERSPEX LID COPERCHIO
E-AU 041 7025-302 1 INSTRUCTION MANUAL (on USB pen drive) MANUALE DI ISTRUZIONE (su chiavetta USB)
CYLINDERS
7025
7125 7026 1 10ml CYLINDER-PISTON ASSEMBLY CILINDRO PISTONE 10ml
7025-30
7125-30 7027 1 30ml CYLINDER-PISTON ASSEMBLY CILINDRO PISTONE 30ml
7025-5
7125-5 7128 1 5ml CYLINDER-PISTON WITH SUITABLE LINK CILINDRO PISTONE 5ml con biella dedicata
7025-1
7125-1 7131 1 1ml CYLINDER-PISTON WITH SUITABLE LINK CILINDRO PISTONE 1ml con biella dedicata
OPTIONAL
7025-150 1 CONNECTION KIT FOR ANESTHESIA SYS-
TEM TO VENTILATOR 7025 KIT CONNESSIONE DA SISTEMA DI ANESTE-
SIA A POMPA DI VENTILAZIONE PER 7025
DATE / / Serial No. IMBALLATO DA / PACKED BY
Universal Input 85-264 VAC, 50-60Hz
IMPORTANT/IMPORTANTE:
FROM: Name Company/Institution
DATE REF.
NOTE
MOD.04 REV 0
Page i
7025 Instruction Manual (Rev.2)
C O N T E N T S
1GENERAL..........................................................................................................1
1.1SYNCHRONIZED START/STOP ON MODEL 7125 ....................................................1
2INSTRUMENT DESCRIPTION...........................................................................1
2.1KINEMATICS..........................................................................................................1
2.2CYLINDER/PISTON &VALVE...................................................................................2
2.3PUMP FRAME........................................................................................................3
3INSTALLATION .................................................................................................3
3.1UNPACKING &PRELIMINARY CHECK ......................................................................3
3.2NOTES ON THE INSTRUCTION MANUAL....................................................................4
3.3BEFORE APPLYING POWER....................................................................................4
3.3.1MAINS SWITCH 4
3.3.2FUSE HOLDER 4
3.3.3MAINS CORD 4
3.4CONNECTIONS ......................................................................................................4
3.5ADDITIONAL SAFETY CONSIDERATION ....................................................................5
4OPERATION ......................................................................................................5
4.1SWITCHING ON.....................................................................................................5
4.2RATE ...................................................................................................................6
4.3STROKE VOLUME..................................................................................................6
4.3.1VOLUME SCALE READING 6
4.4VENTILATION STANDARDS FOR SMALL MAMMALS ...................................................7
4.5TTL START/STOP COMMAND.................................................................................7
4.6SYNCHRONISED START/STOP MODEL 7125............................................................7
4.7CONNECTION TO ANIMAL .......................................................................................8
5MAINTENANCE .................................................................................................8
5.1ELECTRICAL .........................................................................................................8
5.2MECHANICAL........................................................................................................9
5.2.1CHANGING CYLINDER/PISTON ASSEMBLY 9
5.2.2LUBRICATING LINKAGE 10
5.2.3LUBRICATING CYLINDER/PISTON 11
5.2.4LUBRICATING VALVE GEAR 11
5.2.5VARIABLE STROKE DISK 11
5.3LONG INACTIVITY ................................................................................................12
5.4CUSTOMER SUPPORT..........................................................................................13
6CHECKING PUMP PERFORMANCES............................................................13
6.1STROKE VOLUME................................................................................................13
Page ii
7025 Instruction Manual (Rev. 2)
6.2FREQUENCY....................................................................................................... 14
7SERVICING ..................................................................................................... 15
7.1AIR LEAKAGE..................................................................................................... 15
7.2ACCESS TO THE INSIDE OF THE INSTRUMENT......................................................... 16
7.3RATE CALIBRATION............................................................................................ 16
7.4INSTRUMENT SPECIFICATIONS................................................................... 17
8ORDERING INFORMATION............................................................................ 18
8.1OTHER AVAILABLE CONFIGURATIONS .................................................................. 18
8.2START/STOP CONFIGURATIONS ....................................................................... 18
8.3OTHER AVAILABLE CYLINDER/PISTON &ACCESSORIES........................................ 19
8.4TO COMPLETE YOUR EQUIPMENT…..................................................................... 19
9BIBLIOGRAPHY.............................................................................................. 21
FIGURE INDEX
Figure 1 “Linkage” .......................................................................................................................... 2
Figure 2 “Power Module”................................................................................................................ 4
Figure 3 “Front Panel” .................................................................................................................... 5
Figure 4 “Stroke Volume Scale” ..................................................................................................... 6
Figure 5 “Valve Connection to Animal”........................................................................................... 8
Figure 6 “Cylinder/Piston Assembly”.............................................................................................. 9
Figure 7 “Back Side of the Plate”.................................................................................................. 12
Figure 8 “Checking Stroke Volume” ............................................................................................. 14
Figure 9 “Checking Stroke to Stroke Volume”.............................................................................. 14
Figure 10 “Access to the Inside of the Instrument”....................................................................... 16
Page 1
7025 Instruction Manual (Rev. 2)
Rodent Ventilator
Cat. 7 0 2 5 / 7 1 2 5
1 GENERAL
The 7025 Rodent Ventilator is a mechanically unique positive pressure pump, according
to Starling’s ventilation method, designed for use with guinea pigs, rats, mice and small
birds, which features:
Mechanically,
A. a unique valve gear with negligible dead space
B. a novel variable stroke linkage mechanism, without sliding components
C. no protruding elements: both the controls and the tubing connectors are re-
cessed into the chassis
Electrically,
D. digital display of “strokes per minute”
E. TTL start-stop command
F. no electronic noise broadcasting
1.1 Synchronized START/STOP on model 7125
The operation of the Rodent Ventilator may be “paused” by an external TTL logic signal.
For more demanding electrophysiological-pharmacological investigations, an optional
synchronised command is available to START and STOP the Ventilator during forced
inspiration. Ask for special model Cat. 7125.
This feature is particularly useful when coupling the operation of the Ventilator to soft-
ware for physiology or neurophysiology experiments. See paragraph 4.6-Synchronised
Start/Stop .
2 INSTRUMENT DESCRIPTION
2.1 Kinematics
A D.C. geared motor, operated by solid state speed control, is coupled to the cylin-
der/piston assembly, via a unique variable stroke linkage (see datasheet picture & Figure
1 “Linkage”), with the following advantages:
Page 2
7025 Instruction Manual (Rev. 2)
The piston almost touches the cylinder end at each stroke, regardless of the
pre-set volume. This ensures that all air taken into the pump is expelled with
each stroke.
Figure 1 “Linkage”
The stroke volume knob can be adjusted either while the pump is running or at
a standstill. Volume is clearly indicated on a stationary scale rather than on a
mobile slotted link or by graduation marks on the cylinder, either of which
leads to uncomfortable volume setting, the latter adjustable only when the
pump is in motion.
The mechanism does not incorporate the slotted link assembly found on other
ventilators. The reciprocating motion is generated, adjusted and transmitted to
the piston by pivoting joints and rods only.
This lack of sliding friction leads to:
A. practically no wear
B. no backlash, and hence silent operation
C. exact stroke reproducibility
D. minimal maintenance requirements
2.2 Cylinder/Piston & Valve
Four different cylinder/piston assemblies are available: 1, 5, 10 and 30 ml.
Page 3
7025 Instruction Manual (Rev. 2)
Particular care has been taken in finishing the cylinder inside surface (diamond lapping),
to minimize the O-ring wear. The piston fits snugly inside the cylinder and is made airtight
by two conveniently spaced O-rings.
The slide valve is placed perpendicular to the cylinder to minimize dead space, and is
operated by a crank-link mechanism (4-8, see Figure 1 “Linkage”).
The valve is made airtight by an exact fit between the seat and the spindle.
2.3 Pump Frame
The backbone of the instrument is a heavy gauge (10 mm) aluminium plate to which all
mechanisms are fastened.
Figure 1 “Linkage” illustrates the external fittings, namely the valve block (17) the variable
stroke disk (7) and its control knob (14).
The transformer, the geared motor and the electronic printed circuit board are fastened to
the other side of the plate (see paragraph 7.2-Access to the Inside of the Instrument).
The plate is fastened to the left wall (the side of the air ports) by two M4 oval-head
screws. The combination wall/plate forms a solid “T” structure, which glides out from the
rails of an all welded 4-sided cabinet, by simply removing the six M4 screws indicated.
This impeccable design makes all internal and external components totally accessible in
a matter of 30 seconds.
3 INSTALLATION
3.1 Unpacking & Preliminary Check
Check the contents of the shipment for completeness, packing list to hand, and visually
inspect the instrument as soon you take it out of the packaging. Use the Check List sup-
plied.
The functional design of the cabinet which avoids protruding elements is the best known
defence against rough transport handling. If however the instrument is damaged or, after
having tested it, fails to meet rated performances, notify the carrier and our company im-
mediately.
Remove any excess grease you may find smeared on the valve spindle head, kinemat-
ics, etc. to preserve the mechanical components against storage and transport hazards.
Protect the environment!
Dispose of packaging properly, according to existing and ap-
plicable waste management rules and regulations.
Page 4
7025 Instruction Manual (Rev. 2)
3.2 Notes on the Instruction Manual
The 7025 Instruction Manual included in the package (on the USB drive) is necessary for
the correct installation and operation of the instrument.
We recommend reading the manual with attention, as it is essential for the correct instal-
lation and operation of the instrument.
Please save the manual, ready to be consulted by the qualified personnel who use the
instrument. Print it, only if necessary.
Our Instruction Manuals are available as free download on our web, see
http://www.ugobasile.com/support/documentation.html. For any additional information
and/or assistance, you are welcome to contact our Service Department (see paragraph
5.4-Customer Support), specifying the serial number of your instrument.
3.3 Before Applying Power
Consider the Power Module, on the in-
strument back panel, which encompasses
– from left to right - the inlet connection of
the mains cord, the mains switch and the
fuse holder.
Figure 2 “Power Module”
The Ventilator has Universal input 85-264 VAC, 50-60Hz.
3.3.1 Mains Switch
This two-pole toggle switch, which complies with international safety standards, provides
a visual cue, meaning:
OFF when pressed the “O” side
ON when pressed to the “I” side
3.3.2 Fuse Holder
The fuse holder comprises two fuses, one on the live, and the other on the neutral. We
recommend 800 mA timed fuses, type T800 A. To replace the fuses, see paragraph 5.1-
Electrical.
3.3.3 Mains Cord
It is a standard cable, Cat. # E-WP008. Make sure your power outtake is provided with a
reliable ground connection, see also 3.4 & 3.5.
3.4 Connections
Connect the mains cord to a power outtake, provided with a reliable earth connection.
Page 5
7025 Instruction Manual (Rev. 2)
3.5 Additional Safety Consideration
a. Place your Ventilator on a steady flat surface.
b. Do not obstruct free and comfortable access to the power module.
c. Use original accessories and spare parts only, see also paragraph 8-
ORDERING INFORMATION.
d. Immediately disconnect and replace an accidentally damaged mains cable.
e. Do not operate the Ventilator in hazardous environments or outside prescribed
environmental limitations (i.e. 10°C / +40°C, 95% relative humidity, non-
condensing), see also paragraph 7.4-INSTRUMENT SPECIFICATIONS.
f. Do not spray any liquid on the connectors.
g. Use only acceptable, commercially available anaesthetic agents with this ven-
tilator.
UGO BASILE DOES NOT ACCEPT ANY RESPONSIBILITY FOR PROB-LEMS OR
HARM CAUSED TO THINGS OR PERSONS ARISING FROM:
incorrect electrical supply.
incorrect installation procedure.
incorrect or improper use or, in any case, not in accordance with the purpose
for which the instrument has been designed and the warnings brought to your
attention in the instruction manual supplied with the instrument.
replacement of original components, accessories or parts with others not ap-
proved by the manufacturer.
servicing carried out by unauthorized personnel.
4 OPERATION
4.1 Switching On
Switch on the 7025 Venti-
lator by acting the Mains
Switch placed on the left
side wall of the instrument;
see paragraph 3.3.1-Mains
Switch. The digital display
labelled STROKES PER
MINUTE will light up, de-
noting that the instrument
is on.
Familiarize with the stroke
and rate control.
Figure 3 “Front Panel”
Page 6
7025 Instruction Manual (Rev. 2)
4.2 Rate
The speed control potentiometer adjusts the geared motor to the desired speed, which is
indicated on the 3-digit LED display labelled STROKE P.M. (see Figure 3 “Front Panel”).
The speed is unaffected by either mains voltage variations or changes of torque load
brought about by different stroke volumes, different cylinder/piston combinations, airway
alterations, status of the lubrication, etc.
The digital display assures excellent reproducibility: you adjust the actual speed of the
geared motor against a displayed number, in lieu of trying to overlap a pointer and a
scale mark.
The digital reading involves the incertitude of the last figure. E.g., 150 STROKES P.M.
could be any value between 150 and 150.9.
4.3 Stroke Volume
The stroke volume is adjustable via the STROKE VOLUME knob (14,
see Figure 1 “Linkage”) located on the front panel (see Figure 3 “Front
Panel”) while the pump is either running or at standstill.
Volume is clearly indicated by a pointer on a stationary dial (15): see
picture and Figure 4 “Stroke Volume Scale”.
The scale indicates the volume obtained with 10ml cylinder/piston as-
sembly.
Please refer to Table 1 ”Stroke Volume Indication” table to find the vol-
umes obtained when different pistons are used.
See also paragraph 4.4.
Figure 4 “Stroke Volume Scale”
4.3.1 Volume Scale Reading
VOLUME SCALE
READING 1 ml Piston
(reading / 10) ml 5 ml Piston
(reading / 2) ml 10 ml Piston
actual reading ml 30 ml Piston
(reading x 3) ml
1 0.1 0.5 1 3
2 0.2 1 2 6
3 0.3 1.5 3 9
4 0.4 2 4 12
5 0.5 2.5 5 15
6 0.6 3 6 18
7 0.7 3.5 7 21
8 0.8 4 8 24
9 0.9 4.5 9 27
10 1 5 10 30
Table 1 ”Stroke Volume Indication”
Page 7
7025 Instruction Manual (Rev. 2)
4.4 Ventilation Standards for Small Mammals
For a deeper evaluation of all the parameters involved in the artificial ventilation of exper-
imental animals, we suggest the operator to read the basic paper on this subject: L.I.
Kleinman & E.P. Radford: “Ventilation Standards for Small Mammals” J. Appl. Physiol.
19(2): 360-362, 1964 (copies are available on request).
4.5 TTL Start/Stop Command
Delicate electrophysiological-pharmacological investigations may require that the animal
“holds its breath” for a while, to minimize all movements.
For this purpose, operation of the Ventilator may be “paused” by an external TTL logic
signal, provided by miniature jacks to fit 2mm banana plugs located on the control panel
(see Figure 3 “Front Panel”).
A voltage signal, 3 to 25V amplitude, RED JACK positive (max. current drain at 25V = 2.5
mA), causes the Ventilator to stop. Restarting takes place when the signal drops to zero!
Accidental reversing of polarity will not activate the circuit but causes no damage.
A special circuit has been designed, to minimize the mechanical-electrical inertia and
electromagnetic interference which would be unavoidable via crude ON-OFF power
switching.
Pause timing ought not to be too long nor too frequent. Compensating a pause with a
faster rate may help; pause when the lungs are full.
For more demanding electrophysiological-pharmacological investigations, An optional
synchronised command is available to START-STOP the Ventilator, see following par-
agraph.
4.6 Synchronised Start/Stop Model 7125
The operation of Ventilator may be “paused” by an external TTL logic signal. This feature,
available on model 7125 (see paragraph 8-ORDERING INFORMATION) is particularly
useful when the operation of the Ventilator is software controlled.
This special model of Rodent Ventilator is provided with optimal synchronous stop fea-
ture; you will notice two BNC connectors on the left side panel of the Ventilator case.
The BNC marked “SYNCHRON” supplies a square pulse (5V amplitude, 20ms duration)
at each ventilator stroke, when the piston is at the upper dead point (i.e., when the piston
almost touches the cylinder bottom and the lungs are inflated.)
This trigger signal can inform/command a number of recording and/or actuating devic-
es. To obtain the synchronous stop, it is necessary to send a 5V pulse (grounded nega-
tive) to the STOP BNC-connector, for at least 5s.
The Ventilator will automatically restart after a 5 second time interval.
Page 8
7025 Instruction Manual (Rev. 2)
4.7 Connection to Animal
A “Y” connection should be inserted between the tracheal cannula and the two tubes
coming from the pump.
Dead space is minimized if the “Y” is as close to the trachea as possible. See Figure 5
“Valve Connection to Animal”.
Expired air can be recovered via the exhaust tube for sampling, partial recycling, etc.,
see Figure 5 “Valve Connection to Animal”.
Figure 5 “Valve Connection to Animal”
Room air or any anaesthetic gas mixture can be used to feed the pump intake.
5 MAINTENANCE
While any service of the instrument ought to be carried out by Ugo Basile personnel or by
qualified personnel authorized by UGO BASILE organization, this section of the instruc-
tion manuals describes normal maintenance procedures which can be carried out at your
facility.
UNPLUG THE MAINS CORD BEFORE CARRYING OUT
ANY MAINTENANCE JOB!
5.1 Electrical
To inspect and/or replace the fuses, disconnect the mains cable first! Insert a minia-
ture screwdriver in the slot indentation, see paragraph 3.3-Fuse Holder, and snap out the
slide which houses the fuses. We recommend 800mA timed fuses, type T800.
After replacing the fuses, snap in the fuse slide: the mechanical “click” ensures that it is
locked.
Page 9
7025 Instruction Manual (Rev. 2)
5.2 Mechanical
5.2.1 Changing Cylinder/Piston Assembly
To replace the cylinder/piston assembly proceed as follows:
A.
Run the pump until the piston is at a rearward position, that is, away from the
valve block. Using the 3 mm Allen wrench provided, remove the two screws
(18, see Figure 6 “Cylinder/Piston Assembly”), accessible from the air port
side, which fasten the cylinder to the valve block.
Figure 6 “Cylinder/Piston Assembly”
Pull the cylinder outward to detach it from the valve block, about 0.5cm. The
cylinder may require a gentle side knock to pop out, as grease film on its bot-
tom may cause it to stick, especially after long inactivity of the pump.
All cylinder/piston units require an identical sequence of moves to be separat-
ed from the pump mechanism.
However, the 30ml cylinder, having a larger bottom surface, may require a
more energetic knock to separate it from the block. However we recommend
avoiding a Karate chop: repeated tapping may be more effective and less
dangerous to the cylinder integrity.
Page 10
7025 Instruction Manual (Rev. 2)
B.
Remove the 4mm Allen screw (19) from the shaft of the triple joint (2), then lift
the cylinder and piston link assembly in one piece, to disengage the link from
the precision triple joint shaft (20).
C.
Once you have removed the combination cylinder/piston, drive out the piston.
Slacken the gudgeon set screw (21) with the 2 mm Allen wrench provided and
drive out the gudgeon (6).
Remove the gudgeon from the replacement piston and connect the new piston
to the link. Reposition the gudgeon, paying attention to its “flat”, which should
face the set screw.
D.
Insert the replacement piston into its cylinder and position the cylinder onto the
valve block, following the steps outlined in a) and b) in reverse order.
Each cylinder/piston combinations is complete with its dedicated link.
Please make sure you use the correct link when replacing the assembly, store
the link with the piston you are not using.
In case of doubt, ask for details to our after sales service: see 5.4-Customer
Support.
Make sure that:
1.
a film of grease is evenly smeared on the cylinder bottom, to make the fit air-
tight.
2.
the cylinder and block ports match correctly.
3.
the piston link bearing flange (24) faces the triple joint fit (20).
4.
you have not left loose parts lying around!
Insert the piston you are not using in its cylinder, lubricate and store. Complete the as-
sembly with its link, if you have switched from 10 or 30ml to 1 or 5ml piston or vice versa.
5.2.2 Lubricating Linkage
The entire linkage is exposed for immediate visual inspection (see Figure 1 “Linkage”).
Lubricate the swinging link bearing (1), the triple joint (2) and the piston & valve gudgeon
(5-6) every 200 hours of operation by applying 1-2 drops of machine oil of 20-30 grade,
as the one provided with the standard package, see paragraph 8-ORDERING INFOR-
MATION.
Before oiling, please remember to unplug the power cord; you can spout or – worse –
your fingers, may be pinched by the shearing action of the “dynamic trio”, i.e., the 9-10-
11 linking members!
Turn the pump on for 1-2 minutes: the motion and capillary action will combine for a thor-
ough bearing lubrication.
Page 11
7025 Instruction Manual (Rev. 2)
The eccentric (3) and the valve link pin (4) are provided with sealed bearings which are
permanently lubricated and protected from dust: they may require attention after several
thousand hours of operation, at which time a major overhaul of the entire mechanism is
recommended.
5.2.3 Lubricating Cylinder/Piston
Every 200 hours of operation it is wise to lubricate the cylinder and valve gear (see fol-
lowing paragraph). It is possible to inject some grease into the cylinder open end with a
syringe, when the piston is stopped at its most forward position (see also paragraph 6.1-
Stroke Volume for guidance).
However, the O-ring spacing combined with the limited piston travel would lead to an un-
even and limited spreading of grease over the working surface. In particular, the inner O-
ring would hardly get any grease.
Not to speak of the problem of removing residual grease and cleaning. For a flawless job,
it is recommended that you take the piston out, see paragraph 5.2.1-Changing Cylin-
der/Piston Assembly.
Remove the residual grease with a cloth; apply fresh grease to fill up ring grooves. Re-
move any excess. Insert the piston and reassemble the whole.
5.2.4 Lubricating Valve Gear
The valve gear is lubricated via the grease cup (22) placed on the valve block top, see
Figure 6 “Cylinder/Piston Assembly”. A lithium-grease tube is supplied with the standard
package, see 8-ORDERING INFORMATION.
When turning the cap clockwise, the grease contained in the cup is squeezed via two
boreholes into the valve seat, where it is distributed by the tapered sections of the spindle
(13). An even film of grease assures adequate air seal, quiet operation, minimum friction
and wear.
1/4 turn of the cap every 8 hours of operation is sufficient to deliver adequate lubricant.
Occasional inspection of the cup (and consequent refilling if empty!) will prevent the risk
of running dry, which might damage the working surfaces of the spindle and valve seat.
An excess of grease squeezed into the valve will not cause any damage. The pumping
action will squirt the excess into the cylinder or out via the “to animal” port.
If you need to clean excess grease from the air ports, use a cotton swab, not a metallic
tool such as a screwdriver; an untoward movement may cause a scratch in the polished
valve seat or spindle which will compromise the air seal.
5.2.5 Variable Stroke Disk
The variable stroke disk (7) turns on generously dimensioned, permanently lubricated
thrust ball bearings. The pinion/ring gear which rotates the disk is also permanently lubri-
cated (14 & 16).
Page 12
7025 Instruction Manual (Rev. 2)
Both mechanisms may require inspection during major overhaul, after several thousand
hours of operation, just as the previously mentioned (see paragraph 5.2.2-Lubricating
Linkage) valve link pin and eccentric ball bearing.
A large-size fine-pitch (MB12) set screw (25) presses a nylon pad against the bottom sur-
face of the disk. See figure below:
Figure 7 “Back Side of the Plate”
The consequent friction prevents the disk from altering its setting under the torque in-
duced by the linkage motion.
In case the stroke volume knob (14) becomes uncomfortably tight or – on the contrary –
loose to the point that the disk inches from the preset volume by itself, adjust the set
screw accordingly. See paragraph 7.2 for the Access to the Inside of the Instrument.
Bear in mind that as little as 1/8 turn of the set screw affects the “braking action” to a no-
table degree. Tightening the lock nut may cause the set screw to turn a little bit past the
selected setting.
Hold the screw in position with a large screwdriver while tightening the lock nut with an
M14 wrench. A minimum of dexterity is desirable and patience is recommended.
5.3 Long Inactivity
After long storage, the Ventilator may not start to run at the minimum setting (15 S.P.M.):
the torque at that setting may not be sufficient to break away aged lubricant film, in par-
ticular at the piston O-ring/cylinder interface.
After some 10 seconds at the higher speed, the problem will be over.
26
Page 13
7025 Instruction Manual (Rev. 2)
5.4 Customer Support
For any further information you may desire concerning the use and/or maintenance of the
ventilator and accessories, please do not hesitate to contact our service department
(or our local distributor) either directly of via the support page on our website
http://www.ugobasile.com/support.html :
UGO BASILE s.r.l.
Via G. Di Vittorio 2
21036 GEMONIO – Varese, ITALY
Phone : +39 0332 744574
Before sending any instrument to our factory for repair, please contact our logistics
department to obtain a return authorization number (RMA) and shipping/packing instruc-
tions.
We may not be held responsible for damages during transport due to poor packing;
whenever possible, please use the original packing.
6 CHECKING PUMP PERFORMANCES
6.1 Stroke Volume
If you have any reason to suspect that the output of the pump at any given setting is not that giv-
en on the graduation marks on the scale (15), there is a simple accurate method for testing the
calibration:
A. Set the speed control knob to about 30 strokes per minute. Set the pump pis-
ton for a volume of 5 ml (or 15 ml, if the 30 ml cylinder/piston is being used)
and stop the pump motor when the piston reaches its most rearward position,
that is, when the cylinder contains 5 ml (respectively 15 ml) of air.
Incidentally, it may prove to be a bit difficult to stop the piston exactly at the de-
sired max. rearward position, because of the inertia of the geared motor com-
bined with the reaction time of the operator in tripping the power switch off.
It is easier to set the speed control at minimum, 15 S.P.M. or below, and then
quickly turn the knob down to zero, or stop the motion by the TTL command,
see paragraph 4.5-TTL Start/Stop Command, when the piston reaches its de-
sired position.
Page 14
7025 Instruction Manual (Rev. 2)
B.
Attach a clean, freely moving 10ml (or 30ml) glass syringe via a short (5cm)
length of tubing to the stem of a “Y” connector. Adjust the plunger so that 1-2
ml (or 3-6 ml) of air is in the syringe, before attaching the tube.
Figure 8 “Checking Stroke Volume”
C.
Attach the two arms of a “Y” to the “room air” and “to animal” ports of the valve
block. Use short lengths of tubing. This will establish a closed system between
the pump cylinder and the syringe, so that the air will move back and forth from
pump to syringe.
D.
Set the speed at about 30 S.P.M. and observe the plunger excursion as it
moves back and forth. Hold the syringe horizontally to minimize gravity effect
on the plunger travel. The limits of its travel will indicate the actual amount of
air being supplied by the pump at each stroke.
To check stroke to stroke volume, use a freely moving 50 ml glass syringe and
connect it with a short length of Tygon® or rubber tubing to the “to animal” port.
With the piston in the max. stroke position, run the pump at about 40 S.P.M.
and observe how many strokes it takes to get 50 ml. On the 5ml setting, for in-
stance, it should take 10 strokes.
Figure 9 “Checking Stroke to Stroke Volume”
Should the foregoing test show an error in the volume output of the pump, the most
probable cause is air leakage, see paragraph 7.1-Air Leakage.
6.2 Frequency
To check the frequency (strokes per minute), use a stopwatch. Run the pump at 60
S.P.M., i.e., one stroke per second. Count 10 piston strokes then stop the watch.
This manual suits for next models
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