UGO BASILE 7025 Series User manual

UGO BASILE S.R.L.
[email protected] / [e-mail] www. ugobasile .com
instruction manual
Rodent Ventilator
Cat. No. 7025 / 7125

UGO BASILE S.R.L.
Via G. Di Vittorio, 2
21036 GEMONIO, VA, ITALY
Phone: [phone]
instruction manual
Rodent Ventilator
Cat. No. 7025 / 7125
[email protected] / [email protected]
www. ugobasile .com
Serial No.
SAFETY CONSIDERATIONS
ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL SAFE-
TY STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND WARN-
INGS WHICH MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO RETAIN
THE INSTRUMENT IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSON-
NEL, AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UN-
DER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITA-
BLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE
HAZARD INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE IN-
STRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated October 2013
Revision 2

www.ugobasile.com
“We have four of your respirators in our extended
lab and they are wonderful - as is your service”
Dr. Nicholas Price, Monash University
Rodent Ventilator
General
The 7025 Rodent Ventilator is a volume-con-
trolled mechanical ventilator (according to Star-
ling’s ventilator method), designed for use
with rats, guinea pigs, mice and small birds.
The 7025 drive consists of a variable speed geared
motor linked by a novel variable stroke mechanism
to easily interchangeable cylinder/piston assem-
blies.
In particular, the 7025 can be equipped with 5, 10
or 30ml cylinder/piston assembly.
Its precisely regulated geared-motor speed provi-
des the most accurate and reliable stroke rate con-
trol of any respirator available.
The operation of the 7025 may be “paused” by an
external TTL logic signal.
The picture features a Rodent Ventilator 7025, together
with the 6025 for Cat/Rabbit
VENTILATORS and GAS ANESTHESIA
Cat. No. 7025
Best available
Starling
Pumps
THE CHOICE OF
THE CRITICS!
Main Features
l Interchangeable cylinder/piston assemblies (5, 10, 30ml)
l Quiet operation, both acustically and electrically (negligible R.F. broadcasting)
l Reliable mechanics and impeccable nishing: lifelong lasting
l Synchronised START/STOP function available as optional
Ugo Basile: more than 10,000 citations
7025
6025

CHECK-LIST
7025 Rodent Ventilator, 10 ml
7025-30 Rodent Ventilator, 30 ml
7025-5 Rodent Ventilator, 5 ml
7025-1 Rodent Ventilator, 5 ml
7125 Rodent Ventilator, Start/Stop, 10 ml
7125-30 Rodent Ventilator, Start/Stop, 30 ml
7125-5 Rodent Ventilator, Start/Stop, 5 ml
7125-1 Rodent Ventilator, Start/Stop, 1 ml
CLIENTE / CUSTOMER______________________________________________
Ordine No. / Order No. ____________________ Data / Date____/_____/______
UB code
CAT.No.
 Q.ty DESCRIPTION DESCRIZIONE
E-WP 008 1 MAINS CABLE EUROPE CAVO RETE EUROPA
E-WP 008-1 U.S.A. U.S.A.
E-FT 008-1 2 FUSES (T800mA) FUSIBILI (T800mA)
7044 1 Y-CANULA CANULA a Y
M-CM 081-F 7033 1 LITHIUM-GREASE GRASSO AL LITIO
7032 1 PERSPEX LID COPERCHIO
E-AU 041 7025-302 1 INSTRUCTION MANUAL (on USB pen drive) MANUALE DI ISTRUZIONE (su chiavetta USB)
CYLINDERS
7025
7125 7026 1 10ml CYLINDER-PISTON ASSEMBLY CILINDRO PISTONE 10ml
7025-30
7125-30 7027 1 30ml CYLINDER-PISTON ASSEMBLY CILINDRO PISTONE 30ml
7025-5
7125-5 7128 1 5ml CYLINDER-PISTON WITH SUITABLE LINK CILINDRO PISTONE 5ml con biella dedicata
7025-1
7125-1 7131 1 1ml CYLINDER-PISTON WITH SUITABLE LINK CILINDRO PISTONE 1ml con biella dedicata
OPTIONAL
7025-150 1 CONNECTION KIT FOR ANESTHESIA SYS-
TEM TO VENTILATOR 7025 KIT CONNESSIONE DA SISTEMA DI ANESTE-
SIA A POMPA DI VENTILAZIONE PER 7025
DATE / / Serial No. IMBALLATO DA / PACKED BY
Universal Input 85-264 VAC, 50-60Hz
IMPORTANT/IMPORTANTE:
FROM: Name Company/Institution
DATE REF.
NOTE
MOD.04 REV 0

Page i
7025 Instruction Manual (Rev.2)
C O N T E N T S
1GENERAL..........................................................................................................1
1.1SYNCHRONIZED START/STOP ON MODEL 7125 ....................................................1
2INSTRUMENT DESCRIPTION...........................................................................1
2.1KINEMATICS..........................................................................................................1
2.2CYLINDER/PISTON &VALVE...................................................................................2
2.3PUMP FRAME........................................................................................................3
3INSTALLATION .................................................................................................3
3.1UNPACKING &PRELIMINARY CHECK ......................................................................3
3.2NOTES ON THE INSTRUCTION MANUAL....................................................................4
3.3BEFORE APPLYING POWER....................................................................................4
3.3.1MAINS SWITCH 4
3.3.2FUSE HOLDER 4
3.3.3MAINS CORD 4
3.4CONNECTIONS ......................................................................................................4
3.5ADDITIONAL SAFETY CONSIDERATION ....................................................................5
4OPERATION ......................................................................................................5
4.1SWITCHING ON.....................................................................................................5
4.2RATE ...................................................................................................................6
4.3STROKE VOLUME..................................................................................................6
4.3.1VOLUME SCALE READING 6
4.4VENTILATION STANDARDS FOR SMALL MAMMALS ...................................................7
4.5TTL START/STOP COMMAND.................................................................................7
4.6SYNCHRONISED START/STOP MODEL 7125............................................................7
4.7CONNECTION TO ANIMAL .......................................................................................8
5MAINTENANCE .................................................................................................8
5.1ELECTRICAL .........................................................................................................8
5.2MECHANICAL........................................................................................................9
5.2.1CHANGING CYLINDER/PISTON ASSEMBLY 9
5.2.2LUBRICATING LINKAGE 10
5.2.3LUBRICATING CYLINDER/PISTON 11
5.2.4LUBRICATING VALVE GEAR 11
5.2.5VARIABLE STROKE DISK 11
5.3LONG INACTIVITY ................................................................................................12
5.4CUSTOMER SUPPORT..........................................................................................13
6CHECKING PUMP PERFORMANCES............................................................13
6.1STROKE VOLUME................................................................................................13

Page ii
7025 Instruction Manual (Rev. 2)
6.2FREQUENCY....................................................................................................... 14
7SERVICING ..................................................................................................... 15
7.1AIR LEAKAGE..................................................................................................... 15
7.2ACCESS TO THE INSIDE OF THE INSTRUMENT......................................................... 16
7.3RATE CALIBRATION............................................................................................ 16
7.4INSTRUMENT SPECIFICATIONS................................................................... 17
8ORDERING INFORMATION............................................................................ 18
8.1OTHER AVAILABLE CONFIGURATIONS .................................................................. 18
8.2START/STOP CONFIGURATIONS ....................................................................... 18
8.3OTHER AVAILABLE CYLINDER/PISTON &ACCESSORIES........................................ 19
8.4TO COMPLETE YOUR EQUIPMENT…..................................................................... 19
9BIBLIOGRAPHY.............................................................................................. 21
FIGURE INDEX
Figure 1 “Linkage” .......................................................................................................................... 2
Figure 2 “Power Module”................................................................................................................ 4
Figure 3 “Front Panel” .................................................................................................................... 5
Figure 4 “Stroke Volume Scale” ..................................................................................................... 6
Figure 5 “Valve Connection to Animal”........................................................................................... 8
Figure 6 “Cylinder/Piston Assembly”.............................................................................................. 9
Figure 7 “Back Side of the Plate”.................................................................................................. 12
Figure 8 “Checking Stroke Volume” ............................................................................................. 14
Figure 9 “Checking Stroke to Stroke Volume”.............................................................................. 14
Figure 10 “Access to the Inside of the Instrument”....................................................................... 16

Page 1
7025 Instruction Manual (Rev. 2)
Rodent Ventilator
Cat. 7 0 2 5 / 7 1 2 5
1 GENERAL
The 7025 Rodent Ventilator is a mechanically unique positive pressure pump, according
to Starling’s ventilation method, designed for use with guinea pigs, rats, mice and small
birds, which features:
Mechanically,
A. a unique valve gear with negligible dead space
B. a novel variable stroke linkage mechanism, without sliding components
C. no protruding elements: both the controls and the tubing connectors are re-
cessed into the chassis
Electrically,
D. digital display of “strokes per minute”
E. TTL start-stop command
F. no electronic noise broadcasting
1.1 Synchronized START/STOP on model 7125
The operation of the Rodent Ventilator may be “paused” by an external TTL logic signal.
For more demanding electrophysiological-pharmacological investigations, an optional
synchronised command is available to START and STOP the Ventilator during forced
inspiration. Ask for special model Cat. 7125.
This feature is particularly useful when coupling the operation of the Ventilator to soft-
ware for physiology or neurophysiology experiments. See paragraph 4.6-Synchronised
Start/Stop .
2 INSTRUMENT DESCRIPTION
2.1 Kinematics
A D.C. geared motor, operated by solid state speed control, is coupled to the cylin-
der/piston assembly, via a unique variable stroke linkage (see datasheet picture & Figure
1 “Linkage”), with the following advantages:

Page 2
7025 Instruction Manual (Rev. 2)
The piston almost touches the cylinder end at each stroke, regardless of the
pre-set volume. This ensures that all air taken into the pump is expelled with
each stroke.
Figure 1 “Linkage”
The stroke volume knob can be adjusted either while the pump is running or at
a standstill. Volume is clearly indicated on a stationary scale rather than on a
mobile slotted link or by graduation marks on the cylinder, either of which
leads to uncomfortable volume setting, the latter adjustable only when the
pump is in motion.
The mechanism does not incorporate the slotted link assembly found on other
ventilators. The reciprocating motion is generated, adjusted and transmitted to
the piston by pivoting joints and rods only.
This lack of sliding friction leads to:
A. practically no wear
B. no backlash, and hence silent operation
C. exact stroke reproducibility
D. minimal maintenance requirements
2.2 Cylinder/Piston & Valve
Four different cylinder/piston assemblies are available: 1, 5, 10 and 30 ml.

Page 3
7025 Instruction Manual (Rev. 2)
Particular care has been taken in finishing the cylinder inside surface (diamond lapping),
to minimize the O-ring wear. The piston fits snugly inside the cylinder and is made airtight
by two conveniently spaced O-rings.
The slide valve is placed perpendicular to the cylinder to minimize dead space, and is
operated by a crank-link mechanism (4-8, see Figure 1 “Linkage”).
The valve is made airtight by an exact fit between the seat and the spindle.
2.3 Pump Frame
The backbone of the instrument is a heavy gauge (10 mm) aluminium plate to which all
mechanisms are fastened.
Figure 1 “Linkage” illustrates the external fittings, namely the valve block (17) the variable
stroke disk (7) and its control knob (14).
The transformer, the geared motor and the electronic printed circuit board are fastened to
the other side of the plate (see paragraph 7.2-Access to the Inside of the Instrument).
The plate is fastened to the left wall (the side of the air ports) by two M4 oval-head
screws. The combination wall/plate forms a solid “T” structure, which glides out from the
rails of an all welded 4-sided cabinet, by simply removing the six M4 screws indicated.
This impeccable design makes all internal and external components totally accessible in
a matter of 30 seconds.
3 INSTALLATION
3.1 Unpacking & Preliminary Check
Check the contents of the shipment for completeness, packing list to hand, and visually
inspect the instrument as soon you take it out of the packaging. Use the Check List sup-
plied.
The functional design of the cabinet which avoids protruding elements is the best known
defence against rough transport handling. If however the instrument is damaged or, after
having tested it, fails to meet rated performances, notify the carrier and our company im-
mediately.
Remove any excess grease you may find smeared on the valve spindle head, kinemat-
ics, etc. to preserve the mechanical components against storage and transport hazards.
Protect the environment!
Dispose of packaging properly, according to existing and ap-
plicable waste management rules and regulations.

Page 4
7025 Instruction Manual (Rev. 2)
3.2 Notes on the Instruction Manual
The 7025 Instruction Manual included in the package (on the USB drive) is necessary for
the correct installation and operation of the instrument.
We recommend reading the manual with attention, as it is essential for the correct instal-
lation and operation of the instrument.
Please save the manual, ready to be consulted by the qualified personnel who use the
instrument. Print it, only if necessary.
Our Instruction Manuals are available as free download on our web, see
http://www.ugobasile.com/support/documentation.html. For any additional information
and/or assistance, you are welcome to contact our Service Department (see paragraph
5.4-Customer Support), specifying the serial number of your instrument.
3.3 Before Applying Power
Consider the Power Module, on the in-
strument back panel, which encompasses
– from left to right - the inlet connection of
the mains cord, the mains switch and the
fuse holder.
Figure 2 “Power Module”
The Ventilator has Universal input 85-264 VAC, 50-60Hz.
3.3.1 Mains Switch
This two-pole toggle switch, which complies with international safety standards, provides
a visual cue, meaning:
 OFF when pressed the “O” side
 ON when pressed to the “I” side
3.3.2 Fuse Holder
The fuse holder comprises two fuses, one on the live, and the other on the neutral. We
recommend 800 mA timed fuses, type T800 A. To replace the fuses, see paragraph 5.1-
Electrical.
3.3.3 Mains Cord
It is a standard cable, Cat. # E-WP008. Make sure your power outtake is provided with a
reliable ground connection, see also 3.4 & 3.5.
3.4 Connections
Connect the mains cord to a power outtake, provided with a reliable earth connection.