Unitron 14350 User manual

73 Mall Drive, Commack, NY 11725 • 631-543-2000 (P) • 631-589-6975 (F)
www.unitronusa.com • [e-mail]
EXAMET-5
MICROSCOPE SERIES
MANUAL

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 2
CONTENTS
SAFETY NOTES ..................................................................................................................3
CARE AND MAINTENANCE................................................................................................3
INTRODUCTION..................................................................................................................4
UNPACKING AND COMPONENTS.....................................................................................4
COMPONENTS DIAGRAMS................................................................................................6
ASSEMBLY..........................................................................................................................7
DETAILED ASSEMBLY PROCEDURE...........................................................................8-10
ADJUSMENT AND OPERATION
ILLUMINATION.........................................................................................................................11
SELECTING THE LIGHT PATH................................................................................................11
ADJUSTING THE BRACKET....................................................................................................12
FOCUSING...............................................................................................................................12
ADJUSTING THE FOCUSING TENSION .................................................................................12
ADJUSTING THE DIOPTER.....................................................................................................13
ADJUSTING INTERPUPILLARY DISTANCE............................................................................13
ADJUSTING THE STAGE.........................................................................................................13
ADJUSTING THE FIELD DIAPHRAGM (Reflected)..................................................................14
ADJUSTING THE APERTURE DIAPHRAGM (Reflected).........................................................14
ADJUSTING THE OBLIQUE ILLUMINATION (Reflected).........................................................14
USING THE COLOR FILTER (Reflected) .................................................................................15
USING THE SIMPLE POLARIZER (Reflected & Transmitted) .................................................15
CENTERING THE CONDENSER (Transmitted) ......................................................................16
ADJUSTNG THE FIELD DIAPHRAGM (Transmitted) ...............................................................16
ADJUSTING THE APERTURE DIAPHRAGM (Transmitted).....................................................17
USING THE COLOR FILTER (Transmitted)..............................................................................17
USING A MICROSCOPY CAMERA..........................................................................................18
REPLACING THE FUSES ........................................................................................................18
TROUBLESHOOTING...................................................................................................19-20
MAINTENANCE..................................................................................................................21
SERVICE............................................................................................................................21
WARRANTY.......................................................................................................................21
PARTS & ACCESSORIES ............................................................................................22-23

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 3
SAFETY NOTES
1. Open the shipping carton carefully to prevent any accessory, i.e. objectives or eyepieces, from
dropping and being damaged.
2. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments.
3. If any specimen solutions or other liquids splash onto the stage, objective or any other component,
disconnect the power cord immediately and wipe up the spillage. Otherwise, the instrument may be
damaged.
4. LAMP REPLACEMENT -- CAUTION: the glass envelope of the lamp may be extremely
hot. DO NOT attempt to change the lamp before it is completely cooled or without
wearing adequate skin protection.
5. All electrical connectors (power cord) should be inserted into an electrical surge protector to prevent
damage due to voltage fluctuations.
6. FUSE REPLACEMENT -- For safety when replacing the fuse (ONLY replace with the
same size, type and rating of original fuse), be sure the main switch is in the off position,
disconnect the power cord from outlet, and replace the fuse. Reconnect the power cord
and turn unit on.
7. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The
use of a different input voltage other than indicated will cause severe damage to the microscope.
NOTE: Always plug the stereomicroscope power cord into a suitable grounded electrical outlet. A
grounded 3-wire cord is provided.
CARE AND MAINTENANCE
1. Do not attempt to disassemble any component including eyepieces, objectives or focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal surfaces
should be cleaned with a damp cloth. More persistent dirt should be removed using a mild soap
solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an air stream from
an air bulb. If dirt remains on the optical surface, use a soft, lint free cloth or cotton swab dampened
with a lens cleaning solution (available at camera stores). All optical lenses should be swabbed using
a circular motion. A small amount of absorbent cotton wound on the end of a tapered stick makes a
useful tool for cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up
grease making cleaning more difficult.
4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover when not
in use.
5. UNITRON®microscopes are precision instruments which require periodic servicing to maintain
proper performance and to compensate for normal wear. A regular schedule of preventative
maintenance by qualified personnel is highly recommended. Your authorized UNITRON® distributor
can arrange for this service.

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 4
INTRODUCTION
Congratulations on the purchase of your new UNITRON®microscope. UNITRON®microscopes are
engineered and manufactured to the highest quality standards. Your microscope will last a lifetime if
used and maintained properly. UNITRON®microscopes are carefully assembled, inspected and tested
by our staff of trained technicians in our New York facility. Careful quality control procedures ensure each
microscope is of the highest quality prior to shipment.
UNPACKING AND COMPONENTS
Your microscope arrived packed in a molded shipping carton. Do not discard the carton: the shipping
carton should be retained for reshipment of your microscope if needed. Avoid placing the microscope in
dusty surroundings or in high temperature or humid areas as mold and mildew can form. Carefully
remove the microscope from the shipping carton and place the microscope on a flat, vibration-free
surface.
MODELS
Reflected Trinocular
#14350
(Shown with optional camera)
Reflected/Transmitted Trinocular
#14351
Reflected/Transmitted Trinocular
#14351
(Shown with optional camera)

EXAMET-5 SERIES MICROSCOPE
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COMPONENTS DIAGRAM 1 –Reflected Model
Analyzer Slot
Eyepiece
Polarizing
Slot
Stage
Coarse
Focusing Knob
Light Selection
Lever
Fine
Focusing Knob
Variable Light
Intensity Knob
Field Diaphragm
Adjustment Lever
Aperture Diaphragm
Adjustment Lever
Color Filter
Slots
Reflected
Illuminator
Housing
Aperture Diaphragm
Centering Screws
Tension
Adjustment
Collar
Y Axis
Movement Knob
X Axis
Movement Knob
Field Diaphragm
Adjustment Ring
Field Diaphragm
Adjustment Knobs
Stand
Nosepiece
Objective
Camera Port
Trinocular Head
Hexagonal
Tool Slot

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UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 6
COMPONENTS DIAGRAM 2 –Reflected/Transmitted Model
Reflected
Illuminator
Diaphragm
Centering Screws
Power
(ON/OFF)
Switch
Fine Focusing
Knob
Stand
Fuse Holders
Power Cord
Receptacle
Trinocular Head
Camera Port
Eyepiece
Oblique Illumination
Lever
Nosepiece
Objective
Condenser
Focusing
Bracket Group
Condenser
Adjustment
Knob
Variable Light
Intensity Dial
Coarse
Focusing
Knob
Transmitted
Illuminator
Housing
Reflected
Illuminator
Housing

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UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 7
ASSEMBLY
The diagram below shows how to assemble the various modules. The numbers indicate the order of
assembly.
When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
①
②
③
④
⑤
⑥
⑦
⑧
⑨
①Illuminator Housing
②Stage
③Condenser
④Reflected Illuminator
⑤Trinocular Head
⑥Objectives
⑦Eyepieces
⑧Reflected Illuminator Cable
⑨Power Cord

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 8
DETAILED ASSEMBLY
Installing the Transmitted
Lamp Housing (Fig. 1)
Align the guide pins ①with the guide sockets ②and push
the lamp housing securely into the stand.
Mounting the Stage (Fig. 2)
Completely loosen the lock screw ①on the stage.
Carefully mount the stage onto the stage bracket ②by
aligning and then positioning the ring on the underside of the
stage slightly past the back of the ring on the bracket -- then
gently slide the stage onto the bracket ring and secure the
stage by tightening the lock screw.
Installing the Condenser (Fig. 3)
Rotate the coarse focusing knob ①to raise the stage to its
highest position.
Rotate the condenser knob ②lower the bracket for the
condenser to its lowest position.
Completely loosen the condenser lock screw ③.
Swing out the top lens of the condenser ④with the scale
facing forward.
Align the lock screw ⑤on the back side of the condenser
with the slot on the condenser holder ⑥and securely push
the condenser into place.
Tighten the condenser lock screw ③ and raise the condenser
to its highest position by rotating the condenser knob ②.
①
①
②
②
Fig. 1
Fig. 2
①
②
Fig. 3
①
②
③
④
⑤
⑥

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DETAILED ASSEMBLY (continued)
Installing the Reflected Illuminator (Fig. 4)
Completely loosen the lock screw ①of the illuminator.
As shown, position the illuminator above the dovetail hole, tilt
it slightly down on the left and carefully slide the dovetail
under the notches in the dovetail hole and set it into place.
Make the sure it is seated properly, then tighten the lock
screw ①.
Installing the Head (Fig. 5)
Completely loosen the lock screw ①of the head.
As shown, position the head and with it tilted slightly down on
the right, slide the dovetail under the notches in the dovetail
hole and set it into place with the two eyepiece tubes facing
forward. Tighten the lock screw ①.
Installing the Objectives (Fig. 6)
Rotate the coarse focusing knob to move the stage into its
lowest position.
Install the objectives ①into the objective nosepiece from
the lowest magnification to the highest in a clockwise
direction from the rear.
①
Fig. 4
Fig. 5
Fig. 6
①
①

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 10
DETAILED ASSEMBLY (continued)
Installing the Eyepieces (Fig. 7)
Remove the covers ①from the eyepiece tubes and carefully
insert the each eyepiece ②into each of the eyepiece tubes.
Connecting the Power Cord (Fig. 8)
IMPORTANT: Use care when storing the
power cord so that it does not bend or twist.
Use only the power cord provided with your
microscope.
Make sure the power switch is at “0”(OFF).
Insert the female end ①of the power cord into the power
supply socket ②on the base of the stand.
Plug the other end of the power cord into a grounded
outlet.
①
Fig. 7
Fig. 8
①
②
②

EXAMET-5 SERIES MICROSCOPE
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OPERATION
Illumination (Fig. 9-11)
Reflected (Fig. 9)
Turn on the power switch to the ON ( I ) position.
Turn the light adjustment knob ①on the base of the stand
until the illumination is comfortable for observation.
Transmitted (Fig. 10-11)
Turn on the power switch to the ON ( I ) position.
Turn the light adjustment knob ①on the base of the stand
until the illumination is comfortable for observation.
Turn the light adjustment knob (Fig. 11) on the side of the
stand until the illumination is comfortable for observation.
Selecting the Light Path (Fig. 12)
To observe through the binocular head, push the light path
selection lever ①all the way in.
To observe with the trinocular head, pull the light path
selection lever ①all the way out.
Fig. 9
Fig. 10
①
①
Fig. 11
①
Fig. 12
①

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OPERATION (continued)
Adjusting the Bracket (Fig. 13)
For specimens taller than 27mm, adjust stage travel with
the bracket lock screw ①.
Hold the bracket and loosen the bracket lock screw ①with
the included Allen (hex wrench).
Move the bracket to the appropriate position, and tighten
the bracket lock screw ①.
Focusing (Fig. 14)
Place a specimen on the stage and secure it with the clips.
Turn the 5X objective into the light path.
Loosen the coarse tension adjustment knob ①and
observe the specimen through the right eyepiece with your
right eye. Rotate the coarse focusing knob ②until the
image appears in the field of view, then lock the coarse
adjustment knob ①.
To see sharpen or see more detail in the specimen, rotate
the fine focusing knob ③.
NOTE: the coarse adjustment limit stop prevents the
sample from touching the objective.
Adjusting the Focus Tension (Fig. 15)
To adjust tension, hold both left and right focus adjustment
knobs ①with both hands, hold the left knob (to prevent it
from turning), and rotate the right knob clockwise to
increase (tighten) or counterclockwise to decrease (loosen)
the focus knob tension.
After tension adjustment has been completed, always
rotate both adjustment knobs in the same direction.
Fig. 13
Fig. 14
①
②
③
①
Fig. 15
①

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 13
OPERATION (continued)
Adjusting the Diopter (Fig. 16)
After focusing the image with the right eye/eyepiece, ob-
serve the image through the left eye/eyepiece. Rotate the
diopter adjustment collar ①until the image becomes clear.
The diopter range is ±5 and the value aligned with the
scale ②is your diopter setting. Be sure to write down your
diopter setting, as settings will vary from user to user.
Adjusting Interpupillary Distance (Fig. 17)
Different users have different interpupillary distances (this
distance is between the centers of the pupils of each eye).
When the operator changes, it will be necessary to adjust
the interpupillary distance.
While looking through the eyepieces, hold the left and right
eyetubes of the viewing head and adjust the eyetubes by
opening or closing them until the left and right fields of view
coincide completely and you are able to see a complete
circle.
Adjusting the Stage (Fig. 18)
When observing, move the stage by rotating the X ② and
Y ③-axis adjustment knobs. If the moving direction of the
stage is different from the image’s, rotate the stage to
adjust it:
Loosen the stage lock screw ①.Rotate the stage
clockwise or counterclockwise until the moving direction of
the stage is the same as the image’s, then tighten the lock
screw.
Rotation angle:
For right hand, clockwise 90º, counterclockwise 20º.
For Left hand, clockwise 20º, counterclockwise 90º.
①
Fig. 16
①
②
②
Fig. 17
Fig. 18
③

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 14
OPERATION (continued)
Adjusting the Field Diaphragm (Fig. 19-20)
Reflected Illumination
By limiting the amount of light entering the condenser, the
field diaphragm can prevent other light and strengthen the
image contrast. When the image is just on the edge of the
field of view, the objective will obtain the clearest image.
Push the field diaphragm adjustment lever ①all the way in
to minimize the field diaphragm.
Observe the image through the eyepiece and adjust the
field diaphragm centering screws ②on both sides of the
illuminator until the image is in the center (Fig. 20).
Slowly open the field diaphragm adjustment lever ①.
When the image in the diaphragm field in aligned with the
field of view, the field diaphragm is centered.
Adjusting the Aperture Diaphragm (Fig.19&21)
Reflected Illumination
The aperture size is increased or decreased by opening
or closing the aperture diaphragm adjustment lever ③.
When the aperture is closed, the brightness and
resolution are decreased but the contrast and range of
focus are increased. If the aperture diaphragm is opened,
the brightness and resolution are increased; however, the
contrast and range of focus are diminished.
For optimal viewing conditions set the condenser aperture
diaphragm lever to match the magnification of the
objective in the optical path.
Adjusting the Oblique Illumination (Fig. 22)
Reflected Illumination
When the oblique illumination lever ①is pushed all the
way in, the system is in oblique illumination observation;
when pulled all the way out, it is in normal reflected
illumination observation.
To adjust the limit block for various specimen
requirements, loosen the lock screw ②on the limit block
③, then move the limit block according to the direction
shown in Fig. 24 and tighten the lock screw when adjusted
to the appropriate position.
①
②
Fig. 19
③
④
Fig. 20
Image of Aperture Diaphragm
Inside
Track
Edge of
Objective
70-80%
Fig. 21
Fig. 22
②
③
①

EXAMET-5 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 15
OPERATION (continued)
Using the (Optional) Color Filters (Fig. 23)
Reflected Illumination
A color filter can make the background more suitable and
increase the image contrast.
Insert an color filter ①into one of the slots ②as shown.
When not using a color filter, insert the blank filter into the
filter slot.
Using the Simple Polarizer (Fig. 24-25)
Reflected Illumination
The simple polarizer includes the polarizer and the analyzer.
Insert the polarizer ①into the polarizer slot ②of the
illumination system as shown. Be sure to pull out the color
filter when using the polarizer.
Insert the analyzer ③into the analyzer slot ④as shown.
The polarizer and the analyzer are orthoganol when the
360ºrotating analyzer is zero adjusted (or the fixing
analyzer is used).
Dialing the rotatable analyzer drive plate can change the
orthogonal state of the polarized light.
Transmitted Illumination
Insert the polarizer ①into the top receptacle of the field
diaphragm holder ②. (Fig. 25)
Insert the analyzer into the analyzer slot ④(Fig. 24).
④
Fig. 23
②
①
Fig. 24
②
①
③
Fig. 25
①
②

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UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 16
OPERATION (continued)
Centering the Condenser
Transmitted Illumination (Fig. 26-27)
Turn the condenser adjustment knob ①to raise it to its
highest position.
Turn the spanner ②to move the front lens into the light
path.
Rotate the 20x objective into the light path and focus the
specimen.
Rotate the field diaphragm adjustment ring ③to move the
field diaphragm to the smallest position. The image of field
diaphragm can be observed through the eyepiece.
Adjust the condenser centering adjustment screws ④to
center the image of field diaphragm to the field of view.
Open the field diaphragm slowly. If the image is in the
center and inscribed to the field of view, the condenser is
correctly centered (Fig. 27). In use, you can enlarge the
field diaphragm a bit and make the image circumscribed to
the field of view.
Adjusting the Field Diaphragm (Fig. 25)
Transmitted Illumination
By limiting the amount of light entering the condenser, the
field diaphragm can prevent other light and strengthen the
image contrast. When the image is just on the edge of the
field of view, the objective will obtain the clearest image.
Rotate the field diaphragm adjustment ring ②(Fig. 25)
clockwise to enlarge the field diaphragm, counterclockwise
to decrease it.
①
②
Fig. 26
Fig. 27
③
④

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OPERATION (continued)
Adjusting the Aperture Diaphragm
Transmitted Illumination (Fig. 25, 28-29)
The aperture diaphragm ①decides the numerical aperture
of the illumination system. If the N.A. of the illumination
matches the N.A. of the objective ②, you’ll achieve better
resolution and contrast and increase the depth of field.
As the contrast is usually low, adjust the condenser aperture
diaphragm to 70-80% of the objective N.A. being used. If
necessary, remove the eyepiece to observe through the
eyepiece tube. Adjust the aperture diaphragm adjustment
ring ②(Fig. 25) to adjust proportion until you see the image
(Fig. 28).
Use of graduation: set the N.A. of the condenser to 80% of
the N.A. of objective (Fig. 28).
Using the (Optional) Color Filters (Fig. 29)
Transmitted Illumination
A color filter can make the background more suitable and
increase the image contrast.
To insert a filter, place it as shown in Fig. 30 with the rough
side down.
When not using a color filter, insert the blank filter into the
filter slot.
Image of Aperture Diaphragm
Inside
Track
Edge of
Objective
70-80%
Fig. 28
Fig. 29
Fig. 30

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OPERATION (continued)
Using a Microscopy Camera (Fig. 31)
(Optional)
1. Loosen the lock screw ①and remove the dust cap ②on
the camera port.
2. Remove the dust cover ③ on the c-mount adapter and
thread the adapter onto the camera.
3. Install the camera with the c-mount adapter into the photo
port.
4. Tighten the lock screw ①.
5. Pull the light path selection lever ④out to allow light to
enter the camera for observation.
Replacing the Fuse (Fig. 32)
Turn the power switch to “O” (OFF) before replacing the
fuse.
Unplug the power cord from the outlet and disconnect it from
the back of the microscope.
NOTE: there are two fuse holders on the back of the
microscope. The top fuse holder corresponds to the
top/reflected illuminator; the bottom fuse holder corresponds
to the bottom/transmitted illuminator.
Unscrew the fuse holder corresponding to the illuminator
that is not lighting with a flat head ( -- ) screwdriver.
Install a new fuse and re-install the fuse holder and tighten
with the screwdriver.
Plug the power cord into the back of the microscope and
into the wall outlet.
Turn on the microscope ( l ).
Use only the appropriate fuse for replacement: 250v 3.15a
(CAT# 142-30-03).
②
③
④
⑤
①
Fig. 31
①
Fig. 32

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UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 19
TROUBLESHOOTING
Under certain conditions, performance of this unit may be adversely affected by factors other than
defects. If a problem occurs, please review the following list and take remedial action as needed. If you
cannot solve the problem after checking the entire list, please contact your local dealer for assistance.
OPTICAL SYSTEM
Problem
Cause
Solution
The bulb is bright
but it is dark in
field of view
Field diaphragm is not large enough
Largen the field diaphragm
Polarizer or analyzer is used
Pull them out
The beam splitter is in the trinocular
observation position
Move the beam splitter lever to the binocular
observation position
The side of the
field of view is
dark or not even
The nosepiece is not in the right position
Turn the nosepiece into the correct position
Stain or dust has accumulated on the lens
(objective or eyepieces)
Clean the lens
The color filter, polarizer or analyzer is not
in the correct position
Insert them or move out of the light path
Beam splitter is not in right position
Move it into the correct position
Stain or dust is
observed in the
field of view
Stains have accumulated on the specimen
Clean the specimen
Stains have accumulated on the lens
Clean the lens
Unclear image
Cover glass on the specimen slide
Use the one without cover
The specimen and the objective are not
vertical
Adjust it
The aperture is not opened correctly
Adjust it
Stain or dust has accumulated on the lens
of eyepiece
Clean the lens
Beam splitter is not in right position
Move it into the correct position
One side of the
image is dark or
the image moves
while focusing.
The specimen slide is not clamped
Clamp it with the stage clips
The nosepiece is not in the correct position
Turn the nosepiece into the correct position
The eyes feel tired
easily -- the right
field of view
doesn’t
superimpose with
the left
Interpupillary distance is wrong
Adjust the interpupillary distance
Diopter adjustment is wrong
Adjust the diopter
The eyepiece for the right eye is different
from the left one
Use the same eyepieces

EXAMET-5 SERIES MICROSCOPE
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MECHANICAL SYSTEM
Problem
Cause
Solution
The objective
touches the cover
glass while
turning the
nosepiece
Stage is too high
Lower it to an appropriate position
Coarse focusing
knob is too tight
Tension adjustment ring is too tight
Loosen it to an appropriate position
Stage moves and
cannot stay on
the focal plane
Tension adjustment ring is too loose
Tighten it to an appropriate position
Coarse focusing
knob cannot rise
The coarse limit screw is locked
Loosen the screw
The image moves
obviously when
touching the
stage
The stage is fastened incorrectly
Fasten the stage correctly
ELECTRICAL SYSTEM
Problem
Cause
Solution
The bulb does not
work
No power supply
Check the connection of the power cord
The bulb burnt out
Replace it
The field of view
is not bright
enough
The light adjustment knob is not adjusted
correctly
Adjust correctly
The bulb flickers
or the brightness
is not stable
The bulb will burn out soon
Replace with a new one
The wire doesn’t connect well
Connect correctly