Unitron EXAMET-4 User manual

EXAMET-4
MICROSCOPE SERIES
MANUAL
73 Mall Drive, Commack, NY 11725 • 631-543-2000 (P) • 631-589-6975 (F)
www.unitronusa.com • [email protected]

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 2
CONTENTS
SAFETY NOTES ..................................................................................................................3
CARE AND MAINTENANCE................................................................................................3
INTRODUCTION..................................................................................................................4
UNPACKING AND COMPONENTS.....................................................................................4
COMPONENTS DIAGRAMS.............................................................................................5-8
ASSEMBLY..........................................................................................................................9
DETAILED ASSEMBLY PROCEDURE.........................................................................10-12
ADJUSMENT AND OPERATION
ILLUMINATION.........................................................................................................................13
SELECTING THE LIGHT PATH................................................................................................13
FOCUSING...............................................................................................................................14
ADJUSTING THE BRACKET....................................................................................................14
ADJUSTING THE FOCUSING TENSION .................................................................................14
ADJUSTING THE DIOPTER.....................................................................................................15
ADJUSTING INTERPUPILLARY DISTANCE............................................................................15
ADJUSTING THE STAGE.........................................................................................................16
ADJUSTING THE FIELD DIAPHRAGM (Reflected)..................................................................16
ADJUSTING THE APERTURE DIAPHRAGM (Reflected).........................................................17
ADJUSTING THE OBLIQUE ILLUMINATION (Reflected).........................................................17
USING THE COLOR FILTER (Reflected) .................................................................................18
USING THE SIMPLE POLARIZER (Reflected) ........................................................................18
CENTERING THE CONDENSER (Transmitted) ......................................................................19
ADJUSTING THE APERTURE DIAPHRAGM(Transmitted)......................................................20
CENTERING THE FILAMENT (Transmitted) ...........................................................................20
USING THE COLOR FILTER (Transmitted)..............................................................................21
REPLACING THE FUSE(Transmitted) .....................................................................................21
INSTALLING & USING THE C-MOUNT ADAPTER..................................................................21
SPECIFICATIONS..............................................................................................................22
TROUBLESHOOTING...................................................................................................23-24
MAINTENANCE..................................................................................................................25
SERVICE............................................................................................................................25
WARRANTY.......................................................................................................................25

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 3
SAFETY NOTES
1. Open the shipping carton carefully to prevent any accessory, i.e. objectives or eyepieces, from
dropping and being damaged.
2. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments.
3. If any specimen solutions or other liquids splash onto the stage, objective or any other component,
disconnect the power cord immediately and wipe up the spillage. Otherwise, the instrument may be
damaged.
4. LAMP REPLACEMENT -- CAUTION: the glass envelope of the lamp may be extremely
hot. DO NOT attempt to change the lamp before it is completely cooled or without
wearing adequate skin protection.
5. All electrical connectors (power cord) should be inserted into an electrical surge protector to prevent
damage due to voltage fluctuations.
6. FUSE REPLACEMENT -- For safety when replacing the fuse (ONLY replace with the
same size, type and rating of original fuse), be sure the main switch is in the off position,
disconnect the power cord from outlet, and replace the fuse. Reconnect the power cord
and turn unit on.
7. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The
use of a different input voltage other than indicated will cause severe damage to the microscope.
NOTE: Always plug the stereomicroscope power cord into a suitable grounded electrical outlet. A
grounded 3-wire cord is provided.
CARE AND MAINTENANCE
1. Do not attempt to disassemble any component including eyepieces, objectives or focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal surfaces
should be cleaned with a damp cloth. More persistent dirt should be removed using a mild soap
solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an air stream from
an air bulb. If dirt remains on the optical surface, use a soft, lint free cloth or cotton swab dampened
with a lens cleaning solution (available at camera stores). All optical lenses should be swabbed using
a circular motion. A small amount of absorbent cotton wound on the end of a tapered stick makes a
useful tool for cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up
grease making cleaning more difficult.
4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover when not
in use.
5. UNITRON®microscopes are precision instruments which require periodic servicing to maintain
proper performance and to compensate for normal wear. A regular schedule of preventative
maintenance by qualified personnel is highly recommended. Your authorized UNITRON® distributor
can arrange for this service.

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 4
INTRODUCTION
Congratulations on the purchase of your new UNITRON®microscope. UNITRON®microscopes are
engineered and manufactured to the highest quality standards. Your microscope will last a lifetime if
used and maintained properly. UNITRON®microscopes are carefully assembled, inspected and tested
by our staff of trained technicians in our New York facility. Careful quality control procedures ensure each
microscope is of the highest quality prior to shipment.
UNPACKING AND COMPONENTS
Your microscope arrived packed in a molded shipping carton. Do not discard the carton: the shipping
carton should be retained for reshipment of your microscope if needed. Avoid placing the microscope in
dusty surroundings or in high temperature or humid areas as mold and mildew can form. Carefully
remove the microscope from the shipping carton and place the microscope on a flat, vibration-free
surface.
MODELS
Reflected
#14250
Reflected
Trinocular
#14250
Camera is Optional
Reflected/
Transmitted
#14251
Reflected/
Transmitted
Trinocular
#14251
Camera is optional

EXAMET-4 SERIES MICROSCOPE
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COMPONENTS DIAGRAM 1 –Reflected Model
Trinocular
Head
Reflected
Illuminator
Eyepiece
Specimen
Platform
Stage
Coarse
Focusing Knob
Nosepiece
Objective
Stand
Oblique
Illumination Lever
Fine
Focusing Knob
Coarse
Adjustment
Knob
Variable Light
Intensity Knob

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 6
COMPONENTS DIAGRAM 2 –Reflected Model
Lock Screw
Field Diaphragm
Adjustment Lever
Aperture Diaphragm
Adjustment Knob
Aperture Diaphragm
Centering Knob
Field Diaphragm
Centering Lever
Color Filter
Slots
Tension
Adjustment
Collar
Beam Splitter
For Camera Port
Lever
Polarizer Slot
Analyzer Slot
Stage Lock Screw
Bracket Lock Screw
Y Axis
Movement Knob
X Axis
Movement Knob

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 7
COMPONENTS DIAGRAM 3 –Reflected &Transmitted Model
Trinocular
Head
Reflected
Illuminator
Eyepiece
Specimen
Platform
Stage
Coarse
Focusing Knob
Nosepiece
Objective
Stand
Oblique
Illumination Lever
Fine
Focusing Knob
Coarse
Adjustment
Limit Knob
Variable Light
Intensity Knob
Illuminator
Housing
Condenser
Color Filter Base
Field Diaphragm
Adjustment Ring
Condenser Adjustment Knob

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 8
COMPONENTS DIAGRAM 4 –Reflected &Transmitted Model
Camera Port
Lock Screw
Field Diaphragm
Adjustment Lever
Aperture Diaphragm
Adjustment Lever
Color Filter Slot
Aperture Diaphragm
Centering Knob
Field Diaphragm
Centering Knob
Polarizer Slot
Variable Light
Intensity Knob
Neutral Density
Control Knob
Tension
Adjustment
Collar
Beam Splitter
For Camera Port
Lever
Analyzer Slot
Stage Lock
Screw
Condenser Centering
Adjustment Screws
Condenser
Lock Screw
Y Axis
Movement
Knob
X Axis
Movement
Knob

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 9
ASSEMBLY
The diagram below shows how to assemble the various modules. The numbers indicate the order of
assembly.
When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.

EXAMET-4 SERIES MICROSCOPE
UNITRON® 73 Mall Drive, Commack, NY 11725 • 631-543-2000 • www.unitronusa.com 10
DETAILED ASSEMBLY
PROCEDURE
Installing the Lamp (Fig. 1)
Halogen Lamp: 6v 50w. Do not use a lamp of a
different voltage or wattage.
Do not touch the halogen lamp with your bare
fingers. Doing so will shorten the service life of
the lamp. Use a soft, clean cloth or lint free
paper.
When installing the halogen bulb for transmitted
illumination, hold the bulb with a soft clean cloth
or lint free paper and insert the pins into the
sockets .
Center the halogen bulb by rotating knobs and .
Installing the Lamp Housing (Fig. 2)
Align the guide pins with the guide sockets 
and push the lamp housing securely into the stand.
Replacing the Lamp (Fig. 1-2)
When replacing the lamp, turn the power switch to
the off position and remove the power cord.
Allow the lamp house and bulb to completely cool.
Remove the lamp housing by gently pulling it away
from the stand.
Remove the old lamp from the socket and replace
with a 6v 50w halogen lamp (see Installing the
Lamp above).
Mounting the Stage (Fig. 3)
Completely loosen the lock screw on the stage.
Carefully mount the stage onto the stage bracket
by aligning and then positioning the ring on the
underside of the stage slightly past the back of the
ring on the bracket -- then gently slide the stage
onto the bracket ring and secure the stage by
tightening the lock screw.
Fig. 1
Fig. 2
Fig. 3

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DETAILED ASSEMBLY
PROCEDURE (continued)
Installing the Condenser (Fig. 4)
Rotate the coarse focusing knob to raise the
stage to its high position.
Rotate the condenser knob lower the bracket for
the condenser to its lowest position.
Completely loosen the condenser lock screw .
Swing out the top lens of the condenser with the
scale facing forward.
Align the lock screw on the back side of the
condenser with the slot on the condenser holder
and securely push the condenser into place.
Tighten the condenser locks screw and raise the
condenser to its highest position by rotating the
condenser knob.
Installing the Reflected Illuminator
(Fig. 5)
Completely loosen the lock screw of the
illuminator.
As shown, position the illuminator above the
dovetail hole, tilt it slightly down on the left and
carefully slide the dovetail under the notches in the
dovetail hole and set it into place. Make the sure it
is seated properly, then tighten the lock screw .
Installing the Head (Fig. 6)
Completely loosen the lock screw of the head.
As shown, position the head and with it tilted
slightly down on the right, slide the dovetail under
the notches in the dovetail hole and set it into
place with the two eyepiece tubes facing forward.
Tighten the lock screw .
Installing the Eyepieces (Fig. 7)
Remove the covers from the eyepiece tubes
and carefully insert the each eyepiece into each
of the eyepiece tubes.
Fig. 4
Fig. 5
Fig. 6
Fig. 7

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DETAILED ASSEMBLY
PROCEDURE (continued)
Installing the Objectives (Fig. 8)
Rotate the coarse focusing knob to move the
stage into its lowest position.
Install the objectives into the objective
Nosepiece from the lowest magnification to
the highest in a clockwise direction from the
rear.
Connecting the Power Cords (Fig. 9)
IMPORTANT: Use care when storing the
power cord so that it does not bend or twist.
Use only the power cord provided with your
microscope.
Make sure the power switch is at “0”(OFF).
Insert the plug of the reflected illuminator into
the matching socket on the back of the stand,
and tighten the screw to secure it.
Insert the plugof the transmitted illuminator into
the matching socket on the back of the stand.
Insert the female end of the power cord into the
power supply socket on the base of the stand.
Plug the other end of the power cord into a
grounded outlet.
Fig. 8
Fig. 9

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OPERATION
Illumination (Fig. 10-12)
For Reflected-Transmitted Models (Fig. 10-11)
Turn on the power switch to the ON ( I ) position.
Turn the light adjustment knob on the base of
the stand until the illumination is comfortable for
observation.
Turn the light adjustment knob (Fig. 11) on the
side of the stand until the illumination is
comfortable for observation.
For Reflected Models (Fig. 12)
Turn on the power switch to the ON ( I ) position.
Turn the light adjustment knob on the base of
the stand until the illumination is comfortable for
observation.
Selecting the Light Path (Fig. 13)
To observe through the binocular head, push the
beam splitter for camera port lever ①all the way
in.
To observe with the trinocular head, pull the
beam splitter for camera port lever ①all the way
out.
Fig. 10
Fig. 11
Fig. 12
Fig. 13

EXAMET-4 SERIES MICROSCOPE
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OPERATION(continued)
Focusing (Fig. 14)
Place a specimen on the stage and secure it
with the clips.
Turn the 5X objective into the light path.
Loosen the coarse adjustment knob and
observe the specimen through the right
eyepiece with your right eye. Rotate the coarse
focusing knob until the image appears in the
field of view, then lock the coarse adjustment
knob .
To see sharpen or see more detail in the
specimen, rotate the fine focusing knob .
NOTE: the coarse adjustment limit stop
prevents the sample from touching the objective.
Adjusting the Bracket (Fig. 15)
Specimens may be observed up to 25mm. For
specimens over 25mm, you need to adjust the
bracket.
Hold the bracket and loosen the bracket lock
screw with the included Allen (hex wrench).
Move the bracket to the appropriate position,
and tighten the bracket lock screw .
Adjusting the Focus Tension (Fig. 16)
To adjust tension, hold both left and right focus
adjustment knobs ①with both hands, hold the
left knob (to prevent it from turning), and rotate
the right knob clockwise to increase (tighten) or
counterclockwise to decrease (loosen) the focus
knob tension.
After tension adjustment has been completed,
always rotate both adjustment knobs in the
same direction.
Fig. 14
Fig. 15
Fig. 16

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OPERATION(continued)
Adjusting the Diopter (Fig. 17)
After focusing the image with the right
eye/eyepiece, observe the image through the
left eye/eyepiece. Rotate the diopter adjustment
collar until the image becomes clear.
The diopter can range is ±5 and the value
aligned with the scale is your diopter setting. Be
sure to write down your diopter setting, as
settings will vary from user to user.
Adjusting Interpupillary Distance
(Fig. 18)
Different users have different interpupillary
distances (this distance is between the centers
of the pupils of each eye). When the operator
changes, it will be necessary to adjust the
interpupillary distance.
While looking through the eyepieces, hold the
left and right eyetubes of the viewing head and
adjust the eyetubes by opening or closing them
until the left and right fields of view coincide
completely and you are able to see a complete
circle.
Fig. 17
Fig. 18

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OPERATION(continued)
Adjusting the Stage (Fig. 19)
When observing, move the stage by rotating the
X and Y-axis adjustment knobs. If the moving
direction of the stage is different from the
image’s, rotate the stage to adjust it:
Loosen the stage lock screw .
Rotate the stage clockwise or counterclockwise
until the moving direction of the stage is the
same as the image’s, then tighten the lock
screw.
Rotation angle:
For right hand, clockwise 90º, counterclockwise 20º.
For Left hand, clockwise 20º, counterclockwise 90º.
Adjusting the Field Diaphragm
- Reflected Illumination (Fig. 20-21)
By limiting the amount of light entering the
condenser, the field diaphragm can prevent
other light and strengthen the image contrast.
When the image is just on the edge of the field
of view, the objective will obtain the clearest
image.
Push the field diaphragm adjustment lever all
the way in to minimize the field diaphragm.
Observe the image through the eyepiece and
adjust the field diaphragm centering lever until
the image is in the center.
Slowly open the field diaphragm adjustment
lever . When the image in the diaphragm field
in aligned with the field of view, the field
diaphragm is centered.
Fig. 19
Fig. 20
Fig. 21

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OPERATION(continued)
Adjusting the Field Diaphragm
- Reflected Illumination (Fig. 20-21)
By limiting the amount of light entering the
condenser, the field diaphragm can prevent other
light and strengthen the image contrast. When
the image is just on the edge of the field of view,
the objective will obtain the clearest image.
Push the field diaphragm adjustment lever all
the way in to minimize the field diaphragm.
Observe the image through the eyepiece and
adjust the field diaphragm centering lever until
the image is in the center.
Slowly open the field diaphragm adjustment lever
. When the image in the diaphragm field in
aligned with the field of view, the field diaphragm
is centered.
Adjusting the Aperture Diaphragm -
Reflected Illumination (Fig. 22-23)
The aperture size is increased or decreased by
rotating the aperture diaphragm adjustment knob
. When the aperture is closed, the brightness
and resolution are decreased but the contrast and
range of focus are increased. If the aperture
diaphragm is opened, the brightness and
resolution are increased; however, the contrast
and range of focus are diminished.
For optimal viewing conditions set the condenser
aperture diaphragm lever to match the
magnification of the objective in the optical path.
Adjusting the Oblique Illumination -
Reflected Illumination (Fig. 24)
When the oblique illumination lever is pushed
all the way in, the system is in oblique illumination
observation; when pulled all the way out, it is in
normal reflected illumination observation.
To adjust the limit block for various specimen
requirements, loosen the lock screw on the limit
block , then move the limit block according to the
direction shown in Fig. 24 and tighten the lock
screw when adjusted to the appropriate position.
Fig. 22
Fig. 23
Fig. 24

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OPERATION(continued)
Using the Color Filter
- Reflected Illumination (Fig. 20-21)
The color filter can make the background more
suitable and increase the image contrast (See
Fig. 25).
Insert the color filter into the slot as shown in
Fig. 25. Your microscope comes with three color
filters: green, blue and red.
When not using a color filter, insert the blank filter
into the filter slot.
Using the Simple Polarizer -
Reflected Illumination (Fig. 26)
The simple polarizer includes the polarizer and the
analyzer.
Insert the polarizer into the polarizer slot of
the illumination system as shown. Be sure to pull
out the color filter when using the polarizer.
Insert the 360ºrotating analyzer or the fixing
analyzer into the analyzer slot as shown.
The polarizer and the analyzer are orthoganol
when the 360ºrotating analyzer is zero adjusted
(or the fixing analyzer is used).
Dialing the rotatable analyzer drive plate can
change the orthogonal state of the polarized light.
Fig. 25
Fig. 26

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OPERATION(continued)
Centering the Condenser
- Transmitted Illumination (Fig. 27-28)
Turn the condenser adjustment knob to raise it
to its highest position.
Turn the spanner to move the front lens into
the light path.
Rotate the 20x objective into the light path and
focus the specimen.
Rotate the field diaphragm adjustment ring to
move the field diaphragm to the smallest position.
The image of field diaphragm can be observed
through the eyepiece.
Adjust the condenser centering adjustment screw
to center the image of field diaphragm to the
field of view.
Open the field diaphragm slowly. If the image is
in the center and inscribed to the field of view, the
condenser is correctly centered (Fig. 28).
In use, you can enlarge the field diaphragm a bit
and make the image circumscribed to the field of
view.
Field Diaphragm
- Transmitted Illumination
By limiting the amount of light entering the
condenser, the field diaphragm can prevent
other light and strengthen the image contrast.
When the image is just on the edge of the field
of view, the objective will obtain the clearest
image.
Fig. 27
Fig. 28
Fig. 29

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OPERATION(continued)
Aperture Diaphragm
- Transmitted Illumination (Fig. 27-28)
The aperture diaphragm decides the numerical
aperture of the illumination system. If the N.A. of
the illumination matches the N.A. of the objective,
you’ll achieve better resolution and contrast and
increase the depth of field.
As the contrast is usually low, adjust the
condenser aperture diaphragm to 70-80% of the
objective N.A. being used. If necessary, remove
the eyepiece to observe through the eyepiece
tube. Adjust the aperture diaphragm adjustment
ring to adjust proportion until you see the
image (Fig. 29).
Use of graduation: set the N.A. of the condenser
to the 80% of the N.A. of objective (Fig. 30).
Center the Filament
- Transmitted Illumination (Fig. 31-32)
Turn the neutral density knob to OUT and
observe the image of the filament through the
eyepiece by adjusting the coarse focusing knob
(adjust the brightness for comfortable
observation as shown in Fig. 10).
If the filament is not in the center of the field of
view, adjust the two knobs as shown in Fig. 32
until it is centered. The top knob controls the
up and vertical (up/down) movement while the
side knob controls the horizontal (left/right)
movement.
Fig. 30
Fig. 31
Fig. 32