Ushio Picoexplorer PAS-110 User manual

User's Manual and Troubleshooting Guide
PAS-110
Contents of this User’s Manual may change without notice.
Due to design improvements, and/or specication and app changes,
PiCOEXPLORERTM conguration may change without notice.
All company names and products names in this manual are the property of their
respective trademark holders.
Patent Pending
注意書
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USHIO Photo Absorbance Sensor
TM

SAFETY NOTES
DEVICE FEATURES HARDWARE PACKAGE
HARDWARE COMPONENTS
BATTERY INSERTION
TAKING MEASUREMENTS OTHER SCREENS MAINTENANCE
TROUBLE- SHOOTING
WARRANTY AND AFTER-SERVICE
SPECIFICATIONS
Thank you for purchasing the PiCOEXPLORERTM PAS-110 photo absorbance sensor.
This manual contains detailed descriptions, instructions for use, and specifications of this device.
Please print out and store this manual in a safe place, and refer to as needed.
These safety notes are intended to help you use this device properly and safely. These notes contain
essential and crucial instructions that must be followed at all times. Please read and understand these notes
before reading the rest of the manual and guide.
SAFETY NOTES
SAFETY NOTES
WARNING
lDo not use the device near medical equipment. The radio waves emitted by wireless
communication module could have an adverse effect on nearby pacemakers or electrical medical
devices.
lDo not use near automatic doors, alarms, or other automatic electrical control devices.
The wireless communication module could cause malfunctioning in these devices.
lDo not use any USB cables that have been damaged or deformed. Doing so could result in smoke,
overheating, or fire.
lCarefully read and correctly follow the instruction in this user’s manual about the use of USB
cables. Replace any cable that looks defective or damaged.
lDo not wash the device with water, or disassemble or tamper with it in any way.
Doing so could result in electric shock, fire, or smoke.
lWhen powering the device thru a USB connector, use a PC with a limited-energy circuit USB
output, or an AC adapter with a limited-energy circuit used to power PCs.
CAUTION
HANDLING OF PHOTO ABSORBANCE SENSOR
lThis device is for general analytic use. It was not designed for, nor should it be used for, medical
diagnostic purposes requiring very high levels of reliability and/or accuracy.
USHIO INC. cannot be held responsible for any damages or liabilities resulting from the
inappropriate use of this device for such purposes.
lTo avoid eye injury, never look directly at the light source in the device.
lWhen connecting and removing the USB cable, do so carefully; do not jam the connector or yank
the cord.
lAvoid dropping the unit or otherwise exposing to shocks or extreme forces.
HANDLING OF BATTERIES
lFollow all instructions of this manual on the handling and use of dry-cell batteries.
lUse only the battery type specified (AAA type). Other types may damage the unit.
lInsert batteries properly, matching the plus and minus ends to the equivalent connections inside
the unit.
lWhen replacing batteries, always replace all 3 batteries together.
lKeep batteries out of the hands of small children.
lIf the unit will not be used for some time, remove the batteries before storage. Leaking batteries
can cause severe damage.
WARNING
CAUTION
This icon indicates a possible danger of loss of life or injury.
This icon indicates a possible danger of injury to personnel or physical damage.
This icon indicates important information concerning easy-to-make mistakes that can adversely
affect operation and maintenance. Not following the instructions given could result in damage
to the device.
IMPORTANT
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SAFETY NOTES
CAUTION
HANDLING OF BATTERIES
lDo not expose batteries to heat or toss into open flames.
lFor the sake of the environment, dispose of used batteries responsibly and in accordance with
local regulations.
lThe batteries that come packaged with the unit were inserted only for device testing purposes.
They may not last long; replace them as soon as possible.
CAUTION
HANDLING OF A BLUETOOTH DEVICE
lThis is a Bluetooth low-energy compatible device. It cannot be connected with any other wireless
communication protocol.
lThis device has been certified as having the proper wireless system for low-power electronic
communication. Do not remove the label of certification from the device. It is against the law to
tamper with the device by disassembling, and/or altering operation. Doing so may result in
criminal prosecution.
lThis device communicates using a 2.4GHz frequency range. This range is also used by the
following wireless transmitters: commercial, scientific, and medical devices and other similar
devices, wireless transmitters used to detect moving object on factory lines (which require
licensing), low-power wireless transmitters which do not require licensing, and amateur wireless
transmitters, all of the above designated herein as “other wireless transmitters”. Before using,
make sure there is no such wireless transmitter in the vicinity which could cause interference. If
you find that the operation of this device near another wireless transmitter is causing
interference, increase the distance between the devices or if possible, turn off the other device.
IMPORTANT
lDo not use in areas with high levels of dust, oil smoke, steam, damp, or high heat.
lIf the unit gets wet or submerged in liquid, immediately turn off and consult your vendor.
lDirt and other contaminants in the measuring chamber can cause erroneous readings. Before
using, make sure the measuring chamber is clean; if not, carefully wipe away all contaminants
with a cotton swab or gauze/soft cloth.
lDo not swing the device by its strap. This could damage/loosen the water-resistant packing in the
device.
lYou may be subjected to penalties to use this device in the country not authorizing the device
usage due to its restrictions on wireless use.
lCommunications errors or reductions in communication speed may result when using the device
in the proximity of a 2.4GHz wireless LAN. Keep the device and the communicating tablet as close
to each other as possible to avoid this.
SAFETY NOTES
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LAWS AND REGULATIONS / APPROVED STANDARDS
FCC NOTICE
This device complies with part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and
(2) this device must accept any interference received, including interference that may cause undesired operation.
Changes or modifications not expressly approved by the party responsible for compliance could void the user’s
authority to operate the equipment.
Note: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to
part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against harmful interference in a residential
installation.
This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in
accordance with the instructions, may cause harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation.
If this equipment does cause harmful interference to radio or television reception, which can be determined by
turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the
following measures:
—Reorient or relocate the receiving antenna.
—Increase the separation between the equipment and receiver.
—Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.
—Consult the dealer or an experienced radio/TV technician for help.
IC NOTICE
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est conformé à la norme NMB-003 du Canada.
This device complies with Industry Canada licence-exempt RSS standard(s). Operation is subject to the following
two conditions: (1) this device may not cause interference, and (2) this device must accept any interference,
including interference that may cause undesired operation of the device.
Le présent appareil est conforme aux CNR d’Industrie Canada applicables aux appareils radio exempts de licence.
L’exploitation est autorisée aux deux conditions suivantes : (1) l’appareil ne doit pas produire de brouillage, et
(2) l’utilisateur de l’appareil doit accepter tout brouillage radioélectrique subi, même si le brouillage est susceptible
d’en compromettre le fonctionnement.
SAFETY NOTES
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CONTENTS SAFETY NOTES ........... 2
DEVICE FEATURES .................................................................................................... 6
HARDWARE PACKAGE .................................................................................................... 6
HARDWARE COMPONENTS .................................................................................................... 7
BATTERY INSERTION .................................................................................................... 8
TAKING MEASUREMENTS ........... 9
1. Using the Top Screen ........................................................................................11
2. Wireless Connection ..........................................................................................12
3. Graphing the Standard Curve............................................................14
3-1. Measurement Wavelength Range Selection ......14
3-2. Blank Measurement ......................................................................15
3-3. Measuring Known Concentrations ....................17
3-4. Graphing the Standard Curve ....................................19
3-5. Standard Curve Loading Function .................... 20
3-6. Unit Maintenance Function ...........................................21
3-7. Other Functions ................................................................................... 24
4. Taking Measurements .................................................................................... 26
4-1. Manual Measurement .............................................................. 26
4-2. Timed Measurement
(Simplied Kinetic Measurement) ...................... 28
5. Displaying Previous Measurements....................................... 30
6. Saving Data (to a CSV le) ....................................................................... 32
OTHER SCREENS
7. Info Screen ........................................................................................................................... 33
8. Save Screen .......................................................................................................................34
9. Help Screen ....................................................................................................................... 36
MAINTENANCE .................................................................................................37
TROUBLESHOOTING .................................................................................................38
WARRANTY AND AFTER-SERVICE .................................................................................................40
SPECIFICATIONS .................................................................................................42
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HARDWARE PACKAGE
The standard package includes:
(1) PAS-110 Unit.............................................................. 1
(2) Alkaline Dry-Cell Batteries (AAA Type)............ 3
(3) Quick Start Guide (includes warranty)............. 1
DEVICE FEATURES
lThe PICOEXPLORERTM is a portable absorptiometer that uses a white LED as its light source.
lYou can verify PICOEXPLORERTM measurement results on your Smartphone or tablet PC by downloading
a special application that uses Bluetooth communication to check the results of measurement.
lThe high sensitivity yet extremely compact design of this device is the result of USHIO's breakthrough
Silicone Optical Technology (SOTTM).
lMeasurements can be taken directly from unopened PCR tubes, helping to prevent contamination.
DEVICE FEATURES HARDWARE PACKAGE
Alkaline Dry-Cell
Batteries (AAA Type)
注意書
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PA S -110 U ni t
Quick Start Guide (includes warranty)
注意書
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Quick Start Guide
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HARDWARE COMPONENTS
HARDWARE COMPONENTS
Measurement Chamber Cover
Open this cover to insert PCR tube
for sample measurement.
Power Switch
Turns unit on and o.
Battery Cover
Open to insert batteries.
Status Light
lSteady Red:
check battery level
lSteady Purple:
powered and connected via
Bluetooth Low Energy
lBlinking Red:
Low voltage*
lBlinking Blue:
measurement in progress
* Some batteries may fail without
rst blinking.
USB Interface Connector
(micro USB)
For connection with a USB cable.
Can be used to supply power
instead of batteries. Not a data
communication channel.
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BATTERY INSERTION
l The unit does not come with ready-to-use, fully charged batteries. If you find batteries already inserted,
be aware that these are for testing purposes only, and may already be near failure.
Batteries Needed: 3 AAA Type Dry-Cell Batteries
CAUTION
Use only AAA type batteries. Use of other types may damage the unit.
Follow the 3 steps below when replacing batteries.
1. Open the battery cover on the back side of the unit.
2. Insert batteries, aligning the plus and minus (+ and - )
ends of the batteries with the plus and minus markings
on the inside of the battery case.
3. Close the battery cover.
BATTERY INSERTION
CAUTION
Make sure battery cover is securely fastened. A loose cover may allow water to seep into the
unit and cause damage.
Before opening the cover, wipe off any liquid on the outside to prevent see page and possible
damage.
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Top Screen Connection Setting Screen
TAKING MEASUREMENTS
Follow the procedure outlined below for taking basic measurement readings.
1. Turn on unit power. The status light will turn red l.
* If battery level falls too low, the red light will begin blinking.
2. Open the measurement chamber lid and set PCR tube into the measurement chamber.
Firmly close the lid.
TAKING MEASUREMENTS
3. Launch the tablet application
(PAS-110) by tapping the icon.
See the handling precautions included
with the PICOEXPLORERTM for how to
install the application on your device.
P11. 1. Using the Top Screen.
4. Make BLE connection.
P12. 2. Wireless Connection.
* Note that the tube lid
connector should fit snugly
between the two guide
projections at the back of
the chamber.
CAUTION
Do not let any sample liquid from the PCR Tube spill into the unit.
This could damage the optical unit.
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Drawing Standard Curve Screen
Concentration Measurement Screen Measurement History Screen
Standard Curve Graph Screen
6. Measure concentration of the unknown
the sample.
P26. 4. Taking Measurements
7. Check previous measurements.
P30. 5. Displaying Previous Measurements
TAKING MEASUREMENTS
5. Draw a standard curve.
P14. 3. Graphing the Standard Curve
TAKING MEASUREMENTS
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Illustration 1: Top Screen
Draw Standard
Curve Button
Goes to the Draw
Standard Curve
Screen to allow
creation of a
standard curve
<P14>.
Measurement
History Button
Goes to the
Measurement
History Screen to
allow inspection of
previous
measurements
<P30>.
Info Button
Goes to the Info
Screen to show
current device status
<P33>.
Measurement
Button
Goes to the
Measurement Screen
to take
measurements
<P26>.
Help Button
Goes to the Help
Screen <P36>.
Connection
Setting Button
To connect to the
hardware unit, goes
to the connection
setting screen
<P12>.
Return Button
Go back to previous screen.
* Android only
* With iOS (on iPhone or
iPad) a Back button is
located in the upper left
corner of the screen.
App History Button
* Android only
Home Button
Returns to the Home
Screen.
* Android only
Bluetooth
Connection
Status
Unconnected
Battery
Voltage Level
OK
Battery
Voltage Level
Low
TAKING MEASUREMENTS
1. Using the Top Screen
The top screen displays the following buttons.
TAKING MEASUREMENTS
Save Button
Moves to the Save
Screen <P34>. On
this screen you can
also switch display
languages between
English and
Japanese.
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Illustration 2-1: Connection Setting Screen
③Bluetooth Device Address
Shows the last Bluetooth device
connected to.
②Connect Button
Press to connect.
①Device Search
Button
Search for devices
to connect to.
TAKING MEASUREMENTS
2. Wireless Connection
Tap the Connection Setting Button on the top screen to move to this screen (Illustration 2-1).
TAKING MEASUREMENTS
Make sure that
Bluetooth
functionality is
enabled on your
Smartphone or
tablet PC.
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Illustration 2-2:
Device Address Not Found
Illustration 2-3:
Device Address Found
Illustration 2-4:
Device Address Selected
(2) Tap the device address that you wish to connect to.
The background color of the address will change, indicating
selection.
(3) Tap the ②Connect Buttonto begin connecting.
(4) Once the connection is established, tap the Return Button to
return to the top screen.
TAKING MEASUREMENTS
(1)Tap ①Device Search Button to search for photo absorbance sensor unit. If not detected, turn the photo
absorbance sensor off and on, then tap the ①Device Search Button again. If the photo absorbance
sensor is found, its Bluetooth device address will be displayed as shown in Illustration 2-3.
TAKING MEASUREMENTS
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Illustration 3-1: Draw Standard Curve Screen ①
③Input Percentage
of LED Output
Level
①RGB Switch
Buttons
⑤Begin Measure-
ment Button
TAKING MEASUREMENTS
3. Graphing the Standard Curve
When drawing a standard curve, first prepare a set of samples of known concentration.
The procedure is as follows:
1. Measurement wavelength range selection;
2. Blank measurement;
3. Measure known concentration sample data;
4. Verify the standard curve graph.
CAUTION
Before beginning the procedure, be sure to tap the Clear Button to clear previous data.
Tap the Standard Curve Button on the top screen to move to the Draw Standard Curve Screen
(Illustration 3-1).
TAKING MEASUREMENTS
④Blank Measure-
ment Intensity
Display
(Red dotted lines
show where up to 3
samples can be
input.)
②Wavelength
Range Conrm
Button
3-1. Measurement Wavelength Range Selection
(1) Select the wavelength range for the sample to be measured using the ①RGB Switch Buttons.
(2) You can check the Maximum absorption wavelength and wavelength range by tapping the ②
Wavelength Range Confirm Button of hint icon.
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Illustration 3-2:
Scrolling list of LED output levels
Illustration 3-3:
Baseline data input box selected
TAKING MEASUREMENTS
3-2. Blank Measurement
(1) Set the sample for determining the blank concentration into
the measurement chamber.
(2) The ③LED output level is 10% by default, but other levels can
be selected. Select the LED output level as appropriate
according to blank measurement timing.
We recommend first taking a measurement at the 10% default
level, and then making adjustments later <Page 15(5)>.
(3) Do blank measurement. When you tap the ④Blank
Measurement Intensity Display, a black frame appears as
shown in Illustration 3-3.
TAKING MEASUREMENTS
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Illustration 3-4:
Measured value displayed in white
font
Illustration 3-5:
Data Clear Conrmation Notice
TAKING MEASUREMENTS
(4) Blank measurement begins when you tap the ⑤Measure
Button. The measured blank intensity is displayed in the black
frame, and the absorption value (0.000) is displayed in the
frame on the right (Illustration 3-4).
At this time R, G, and B data are simultaneously measured.
When measurement is finished, the value will be displayed in
white font. This data will be used to make the graph of the
curve <Page 19> .
*The measured intensity indicates the amount of light passing
through the sample.
Absorption is calculated based on the blank measurement
intensity and the measured intensity of the sample.
(5) The blank measurement intensity should fall in the range of
30000 to 60000. If the value is lower, raise the LED output
level; see (1) on page 14. Also, tap the ①RGB Switch Buttons
and make sure that the blank measurement intensity values
for R, G, and B are not all 65535. Values of 65535 indicate that
the measurement data is saturated, so absorption cannot be
properly measured.
(6) If the measured intensity is 65535, return to (2), lower the
LED output level, and then measure again. When the level is
changed, a prompt like that shown in Illustration 3-5 is
displayed to request confirmation that data is to be cleared;
select “Yes”.
(7) This completes blank measurement.
TAKING MEASUREMENTS
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Illustration 3-6: Draw Standard Curve Screen ②
TAKING MEASUREMENTS
3-3. Measuring Known Concentrations
Using the same Draw Standard Curve Screen (Illustration 3-6), measure a series of samples of known
concentrations.
(1) Set a sample into the measurement chamber of the photo absorbance sensor.
TAKING MEASUREMENTS
⑥Standard Curve
Graph Display
Button
⑤Absorbance
Value Display
(Blue outline: values
multiplied by an
absorbance
multiplier)
①Concentration
Units Select
Button
②Known Concen-
tration Input
Area
(Always enter
concentration values
in ascending order in
Input Areas 1→5.)
③Known Concen-
tration Sample
Measurement
Intensity Display
(Up to 3
measurements can
be made on the
sample, displayed
inside the dotted red
line.)
④Measurement
Button
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Illustration 3-7:
Selection of Concentration Units
Illustration 3-8:
Results of Standard Curve
Measurement Procedure
TAKING MEASUREMENTS
(2) Tap the ①Concentration Units Select Button to choose the
units in which the measurements will be made (Illustration
3-7). Tap the OK to confirm.
Initially, four unit variations are registered (mg/mL, μg/mL, ng/
mL, and %). See pages 18-22 for how to add new units.
(3) Tap the ②Known Concentration Input Area and input the
known concentration value.
(4) Tap the ③Known Concentration Sample Measurement
Intensity Display to display the black frame.
(5) Tap the ④Measure Button to begin measurement. The
measured value will be displayed in white font.
(6) The ⑤Absorbance Value Display will show the actual
calculated absorbance.
(7) When this measurement is finished, remove the current
sample in the photo absorbance sensor, place the next sample
in, return to step (1), and repeat the process.
(8) When all the samples have been measured, tap the ⑥
Standard Curve Graph Display Button and confirm the
graphed results. The values displayed in white font (Illustration
3-8) are the ones displayed on the standard curve <Page 19>.
If you wish to a measurement from the graph, remove it by a
long press on that data’s display box.
(9) When you return to the top screen and proceed to “Measure”,
the unknown sample is measured using the created standard
curve <Page 25>. The standard curve data is stored together
with the measurement data <Page 31>.
* The standard curve cannot be saved by itself.
TAKING MEASUREMENTS
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Illustration 3-9:
Standard Curve Graph Screen
Illustration 3-10:
Fitting a Line to the
Measured Data Points
TAKING MEASUREMENTS
3-4. Graphing the Standard Curve
(1) When you tap the Standard Curve Graph Display Button, a
graph appears indicating concentration on the horizontal axis
and absorption on the vertical axis (Illustration 3-9).
①Standard Curve
Graph Switch
Button
(2) When you tap the ① Standard Curve
Graph Switch Button, graph displayed
changes to show a least squares linear
regression line for the data (Illustration
3-10). Also, the correlation coefficient
(R2) is displayed at ②. This value
indicates the linearity of the data how
well the least squares line fits the data
is indicated by this value.
(3) All of the RGB graphs appear in the
initial display. Graph display of each of
the RGB graphs can be switched on or
off using the ③ RGB Switch Buttons.
TAKING MEASUREMENTS
②Correlation
Coecient
Display Area
③RGB Switch
Buttons
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SAFETY NOTES
DEVICE FEATURES HARDWARE PACKAGE
HARDWARE COMPONENTS
BATTERY INSERTION
TAKING MEASUREMENTS OTHER SCREENS MAINTENANCE
TROUBLE- SHOOTING
WARRANTY AND AFTER-SERVICE
SPECIFICATIONS
TAKING MEASUREMENTS
3-5. Standard Curve Loading Function
Here we explain how to recall stored standard curve data that was used during earlier measurement for
reuse.
(1)Tap the ①Select Standard Curve
Data to display the standard curve
data selection screen.
TAKING MEASUREMENTS
(2) Select the standard curve data and tap the ①Load button. The data loaded from the CSV file is
displayed on the standard curve creation screen.
If the added unit is unavailable after saving the standard curve data, “mg/mL” will be selected.
If you add the unit again on the unit maintenance screen, the unit will be available.
* If you load a standard curve created on another device, there is possibility not to perform accurate measurement.
①Select Standard
Curve Data
②Select Standard
Curve Data
①Load Button
20 Copyright © 2016, USHIO INC. All Rights Reserved.