UVITEC – Cambridge 6/225
EXPOSURE MENU ......................................................................................................................................................44
Access to the Exposure modules ............................................................................................................................... 44
Auto-exposure ........................................................................................................................................................... 46
Manual exposure ...................................................................................................................................................... 49
Serial exposure .......................................................................................................................................................... 52
Focus point adjustment and motorized zoom controls ............................................................................................. 54
ANALYSIS MENU ........................................................................................................................................................56
Access to the analysis modules ................................................................................................................................. 56
M
OLECULAR WEIGHT ANALYSIS MODULE
..........................................................................................................................58
Objectives and output ......................................................................................................................................................58
Molecular weight module (MW) operating environment ........................................................................................59
Toolbar in details..................................................................................................................................................................62
1 -
D
E T E C T
............................................................................................................................................................. 69
A – Edit lane ..........................................................................................................................................................................69
B – Edit band .........................................................................................................................................................................73
– Add marker values ......................................................................................................................................................77
2 -
A
N A L Y S E
–
M
O L E C U L A R W E G H T
........................................................................................................... 82
A – Molecular weight results .............................................................................................................................................82
Return to the 1D acquisition module ........................................................................................................................ 83
C
OLONY COUNTING ANALYSIS MODULE
............................................................................................................................84
Objectives and output ......................................................................................................................................................84
olony counting ( ) module operating environment ..........................................................................................85
Toolbar in details..................................................................................................................................................................87
A
U T O M A T C C O U N T N G
.................................................................................................................................... 94
Detection ..............................................................................................................................................................................94
Return to the 1D acquisition module ........................................................................................................................ 95
M
A N U A L C O U N T N G
........................................................................................................................................... 96
Manual counting .................................................................................................................................................................96
Return to the 1D acquisition module ........................................................................................................................ 97
D
ISTANCE CALCULATION MODULE
...................................................................................................................................98
Objectives and output ......................................................................................................................................................98
Distance calculation module operating environment .............................................................................................99
Toolbar in details................................................................................................................................................................101
1-
D
E T E C T
......................................................................................................................................................... 108
A – Edit lane ........................................................................................................................................................................108
B – Edit band .......................................................................................................................................................................111
– Distance .......................................................................................................................................................................114
2 -
A
N A L Y S E
–
D
S T A N C E
............................................................................................................................... 117
A – Distance results ...........................................................................................................................................................117
Return to the 1D acquisition module ...................................................................................................................... 118
O
PTICAL DENSITY
1D
ANALYSIS MODULE
........................................................................................................................ 119
Objectives and output ....................................................................................................................................................119
Optical density / 1D (OD-1D) operating environment ............................................................................................119
Toolbar in details................................................................................................................................................................122
1-
W
N D O W D E F N T O N
.............................................................................................................................. 128
A – Lane definition ............................................................................................................................................................128
B – Background subtraction ...........................................................................................................................................131
–Spot separation ...........................................................................................................................................................136
2-
A
N A L Y S E
–
S
P O T Q U A N T F C A T O N
................................................................................................... 140
Principles of quantification .............................................................................................................................................140
A –Quantity of reference ................................................................................................................................................141
Return to the 1D acquisition module ...........................................................................................................................144
EDIT MENU .............................................................................................................................................................. 145
Access to the Edit module ....................................................................................................................................... 145
Text ......................................................................................................................................................................... 146
Crop ......................................................................................................................................................................... 150
Rotation .................................................................................................................................................................. 151
Pseudo color ............................................................................................................................................................ 152
