GE AKTA avant User manual

ÄKTA™avant
Operating Instructions
Original instructions

Table of Contents
41 Introduction ..........................................................................................................
51.1 About this manual ................................................................................................................................
61.2 Important user information .............................................................................................................
81.3 Regulatory information ......................................................................................................................
91.3.1 EU Directives ....................................................................................................................................
101.3.2 Eurasian Customs Union ............................................................................................................
111.3.3 Regulations for USA and Canada ...........................................................................................
121.3.4 Other regulations and standards ............................................................................................
141.4 Associated Documentation .............................................................................................................
172 Safety Instructions ...............................................................................................
182.1 Safety Precautions ...............................................................................................................................
272.2 Labels .........................................................................................................................................................
302.3 Emergency procedures ......................................................................................................................
332.4 Recycling information .........................................................................................................................
342.5 Declaration of Hazardous Substances (DoHS) ........................................................................
363 System description ..............................................................................................
373.1 ÄKTA avant instrument overview ..................................................................................................
463.2 UNICORN software ...............................................................................................................................
473.2.1 UNICORN software overview ....................................................................................................
493.2.2 The System Control module ......................................................................................................
514 Installation ............................................................................................................
524.1 Site preparation .....................................................................................................................................
534.1.1 Delivery and storage ....................................................................................................................
554.1.2 Room requirements ......................................................................................................................
584.1.3 Site environment ............................................................................................................................
604.1.4 Power requirements .....................................................................................................................
614.1.5 Computer requirements ..............................................................................................................
634.1.6 Required materials ........................................................................................................................
664.2 Hardware installation .........................................................................................................................
684.2.1 Install the computer equipment ..............................................................................................
694.2.2 Connect the system units ...........................................................................................................
734.2.3 Prepare waste tubing ...................................................................................................................
764.2.4 Install the Barcode Scanner 2-D and the pH electrode .................................................
774.2.5 Prepare the pump rinsing system ...........................................................................................
804.2.6 Start the instrument and the computer ...............................................................................
814.3 Software installation ...........................................................................................................................
824.4 Start UNICORN and connect to system ......................................................................................
854.5 Prime inlets and purge pump heads ...........................................................................................
864.5.1 Prime buffer inlets and purge system pumps ...................................................................
934.5.2 Prime sample inlets and purge Sample Pump ..................................................................
984.5.3 Prime Q inlets ...................................................................................................................................
1024.6 Performance tests ................................................................................................................................
2 ÄKTA avant Operating Instructions 29101556 AB
Table of Contents

1035 Prepare the system for a run .............................................................................
1045.1 Before you prepare the system ......................................................................................................
1065.2 Prepare the flowpath ..........................................................................................................................
1115.3 Prime buffer inlets and purge system pumps .........................................................................
1125.4 Connect a column ................................................................................................................................
1175.5 Set pressure alarms .............................................................................................................................
1195.6 Calibrate the pH monitor ..................................................................................................................
1215.7 Prepare the built-in fraction collector .........................................................................................
1285.8 Prepare for a run at cold temperature .......................................................................................
1296 Run a method ........................................................................................................
1306.1 Before you start .....................................................................................................................................
1336.2 Applying the sample ............................................................................................................................
1366.3 Start a method run ..............................................................................................................................
1426.4 Monitor the run ......................................................................................................................................
1456.5 After run procedures ...........................................................................................................................
1487 Maintenance .........................................................................................................
1497.1 Maintenance program .......................................................................................................................
152
7.2 Replace pump piston seal, O-rings, and rinse membrane of Pump P9 or Pump
P9H ..............................................................................................................................................................
1637.3 Replace pump piston seal, O-ring, and rinse membrane of Pump P9-S ....................
1717.4 Replace pump pistons ........................................................................................................................
1737.5 Clean pump head check valves .....................................................................................................
1767.6 Cleaning before planned service ...................................................................................................
1778 Reference Information ........................................................................................
1788.1 System specifications .........................................................................................................................
1808.2 Chemical resistance guide ...............................................................................................................
1818.2.1 General information about biocompatibility and chemical resistance ..................
1838.2.2 Chemical resistance specifications ........................................................................................
1878.3 Ordering information ..........................................................................................................................
1988.4 Health and Safety Declaration Form ...........................................................................................
200Index .......................................................................................................................
ÄKTA avant Operating Instructions 29101556 AB 3
Table of Contents

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regula-
tory information, intended use of the ÄKTA avant instrument, and lists of associated
documentation.
In this chapter
See pageSection
51.1 About this manual
61.2 Important user information
81.3 Regulatory information
141.4 Associated Documentation
4 ÄKTA avant Operating Instructions 29101556 AB
1 Introduction

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover ÄKTA avant 25 and ÄKTA avant 150 instruments, using
previously created methods in UNICORN software 6.3.2 and later.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates items in a
group, for example Flowpath:Injection valve refers to the Injection valve item in the
Flowpath group.
Hardware items are identified in the text by bold text (for example, the Power button).
ÄKTA avant Operating Instructions 29101556 AB 5
1 Introduction
1.1 About this manual