UVITEC Cambridge UVIDOC HD6 Setup guide

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UVIDOC©HD6
QUICK START USER GUIDE

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UVIDOC©HD6
QUICK START USER GUIDE –V17
The FULL version of this USER GUIDE –V17 is available on your CD.
Thank you
Dear Customer,
On behalf of Uvitec Ltd, we would like to thank you for choosing the UVIDOC HD6 imaging system.
In order to learn the capabilities of your UVIDOC HD6 imaging system, we kindly ask you to read this manual. This
manual details how to install and to operate the hardware and the software components.
Uvitec Ltd is dedicated to your satisfaction and we will be pleased to answer any question you may have. We are also
very receptive to your suggestions. Many of the new features and enhancements in this system are a direct result of
conversations with our customers. Please do not hesitate to contact us to let us know what you would like to see in the
next version of this system.
You can contact us at the following addresses:
Email: [e-mail]
Contact address: UVItec Limited
Unit 36, St John's Innovation Centre
Cowley Road
Cambridge
CB4 0WS
United Kingdom
T. [phone]
F. [phone]
Email: [email protected]
Do not hesitate to visit our website at www.uvitec.co.uk

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Table of contents
Trademarks .............................................................................................................................................................................5
Introduction ............................................................................................................................................................................6
Prerequisites .......................................................................................................................................................................6
About the UVIDOC imaging system ....................................................................................................................................6
Warranty.............................................................................................................................................................................7
Safety instruction....................................................................................................................................................................8
Introduction ........................................................................................................................................................................8
Safety notices......................................................................................................................................................................8
General precaution .............................................................................................................................................................9
Important UV safety information .....................................................................................................................................11
Important electrical power safety information ................................................................................................................13
UVIDOC HD6 system installation ..........................................................................................................................................16
Unpacking the system.......................................................................................................................................................16
Installing the hardware.....................................................................................................................................................18
Environmental conditions.............................................................................................................................................18
Imager back side ...........................................................................................................................................................19
Operating instruction............................................................................................................................................................21
Warning while using the darkroom ..................................................................................................................................21
Imager front side –Door open......................................................................................................................................22
Filter wheel ...................................................................................................................................................................22
System operation..................................................................................................................................................................23
Introduction ......................................................................................................................................................................23
Start the UVIDOC HD6 system & software .......................................................................................................................24
Capture an image..............................................................................................................................................................25
Fluorescence image acquisition....................................................................................................................................25
Image capture workflow...............................................................................................................................................25
Choose the application type .........................................................................................................................................26
Acquire the image.........................................................................................................................................................27

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Save or print the image.................................................................................................................................................32
The Application protocol.......................................................................................................................................................36
What is an Application protocol?......................................................................................................................................36
How to access the application protocol?..........................................................................................................................37
Advanced exposure –Exposure predefined set-up......................................................................................................38
Preview –Live preview pre-defined parameters..........................................................................................................42
Display –Image display pre-defined parameters .........................................................................................................43
Printing –Image printing pre-defined parameters.......................................................................................................46
General –Image file pre-defined parameters ..............................................................................................................47
The System settings ..............................................................................................................................................................49
The general system settings..............................................................................................................................................50
Windows parameters........................................................................................................................................................51
Lock / unlock the Uvidoc system..................................................................................................................................51
Add a printer.................................................................................................................................................................51
Set the network parameters.........................................................................................................................................51
Connect to the Uvidoc from your computer........................................................................................................................53
Connect using your internet browser...............................................................................................................................53
Connect using the Uvitec1D software ..............................................................................................................................53
Update the Uvidoc HD6 firmware: ...................................................................................................................................56
Technical information.......................................................................................................................................................57
Electrical specifications.................................................................................................................................................57
Climatic conditions........................................................................................................................................................57
Spare Parts ........................................................................................................................................................................58
General advices.............................................................................................................................................................58
Spare parts –UVIDOC-HD6...........................................................................................................................................58
Warranty...........................................................................................................................................................................59
Conformity ........................................................................................................................................................................61

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Notice
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical,
including photocopy, recording, or any information storage or retrieval system, without permission in writing from
Uvitec Ltd.
Uvitec Ltd reserves the right to modify its products and services at any time without prior notice.
The contents of this user guide are subject to change without notice. This user guide has been prepared with utmost
care. Uvitec Ltd assumes no liability for errors or omissions, or for any damage resulting from the application or use of
this information. If you have any questions of find errors, omissions, etc., please contact us.
This instrument is suitable for research use only. Some techniques or reagents for generating and / or detecting light in
biological samples are patented and may require licenses from third parties. Users have to independently determine for
themselves whether their activities infringe any valid patent. Uvitec Ltd cannot be held responsible for patent
infringement resulting by the inappropriate user activities.
All goods and services are sold subject to the terms and conditions of sale of Uvitec Ltd. A copy of these terms and
conditions is available on request.
Copyright © 2004-2017 by Uvitec Ltd All rights reserved.
Trademarks
Windows, Windows 10, Windows 8, Windows 7, Windows XP, Windows Vista, Excel, Microsoft, and Power Point are
trademarks of Microsoft Corporation.
Intel Pentium, Intel Core are trademarks of Intel Corporation.
FireWire, Mac, and Mac OS X are trademarks of Apple Inc.
Coomassie is a trademark of BASF Aktiengesellschaft.
Amersham, Cy and ECL Plex are trademarks of General Electric Company.
Alexa Fluor, Coomassie Fluor, Q-dot, SYBR Safe, SYBR Green, SYBR Gold, and SYPRO are trademarks of Life Technologies
Corporation.
Gel-Green and Gel-Red are trademarks of Biotium, Inc.
Dy-Light and Krypton are trademarks of Thermo Fisher Scientific Inc.
Other brands or product names are trademarks of their respective holders.

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Introduction
Prerequisites
The User Guide provides you with the instructions needed to operate, and maintain the UVIDOC HD6 imaging system in
a safe way.
In order to operate the UVIDOC HD6 imaging system in the appropriate way, the following prerequisites must be
fulfilled:
• You have read and understood the safety instructions in this User Guide, particularly in regards of UV danger.
•You should be qualified to use general laboratory equipment and to handle biological materials.
•You should use the instrument only for the intended purpose of gel and blot image acquisition in research
laboratories.
•You accept the terms and conditions of the end user license agreement
•The instrument is connected to a grounded power source and to a circuit breaker.
• The instrument is installed by a Uvitec Ltd representatives.
•This instrument is suitable for research use only and shall not be used in clinical procedures, or for diagnostic
purposes.
•Some techniques or reagents for generating and / or detecting light in biological samples are patented and may
require licenses from third parties. Users should independently determine for themselves whether their activities
infringe any valid patent before operating the UVIDOC imaging system.
•Some UVIDOC HD6 configurations involve UV illumination. This instrument should be used only by trained personnel
who know the health risks associated with the UV radiation normally associated with this instrument. Users should be
trained on the appropriate personal protection gear for working with UV light to minimize UV exposure.
About the UVIDOC imaging system
The UVIDOC imaging system is a scientific instrument designed to capture fluorescence or colorimetric gel images. The
UVIDOC system uses the UVITEC-1D software to control image capture and optimization for selected applications
A scientific camera is used to capture high resolution digital images of protein and DNA bands in gels obtained by
electrophoresis separation methods. The instrument can capture images of fluorescent, and colorimetric samples,
depending on the system configuration.
The instrument can be used for research purposes in the academia and life sciences industry. The instrument cannot be
used for diagnostic purpose.
UVIDOC offers exquisite precision and resolution, which mean reliable results for both quantification and
documentation. The advanced imaging electronics has been developed by our experts especially for your scientific
applications. This association of our exclusive electronic, high-quality optics and advanced software delivers outstanding
performance. With UVIDOC, you simply reach the lowest limits of detection on all of your samples.
The UVIDOC system is supply with the UVITEC-1D software for image quantification and FTP access to the UVIDOC
system.

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Warranty
The UVIDOC imaging system is warranted against faulty construction or defective material for a period of two years from
the Uvitec Ltd invoicing date. If any defect occurs in the instrument during this warranty period, Uvitec Ltd will repair or
replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded:
•Defects caused by improper operation, incorrect use or bad maintenance
•Repair or modification done by anyone other than Uvitec Ltd or the company’s authorized agent
•Use of spare parts supplied by anyone other than Uvitec Ltd.
•Damage caused by accident, misuse or disaster
•Corrosion caused by improper solvents or samples
This instrument should not be modified or altered in any way. Modification or alteration of this instrument will:
1. Void the manufacturer’s warranty.
2. Void the conformity certifications.
3. Create a potential safety hazard.
The light sources (LED panel, UV tubes …), the filters, the power supplies, the batteries, and the consumables are not
covered by our warranty. The use of consumable products or non-original spare parts not recommended by our service
department is at the user's own risk and therefore automatically invalidates the warranty.
We reserve the right to decide where the faulty goods will be repaired (in our workshop or elsewhere), and whether or
not the faulty part is to be replaced; all other freight charges incurred being at the cost of the purchaser. Returned
goods will not be accepted for repair unless previous written authorization is obtained from our service department. A
request for authorization must be accompanied by an itemized list of products, model numbers and the corresponding
invoice numbers under which they were originally shipped. All returned goods should have a certificate of
decontamination. The Buyer must bear all costs and risks incurred during the transportation of the goods from their
collection at Uvitec Ltd factory. In the case Uvitec Ltd incorporates some devices or equipment from another supplier in
the manufacture of its products, the extent and the duration of the warranty will be those conceded by the suppliers or
sellers.
Uvitec Ltd cannot be held responsible for any loss, bodily injury or material accident incurred by any failure of this
supply, whatever the origin of this failure may be. Uvitec Ltd is not responsible for any injury or damage caused by use of
this instrument for purposes other than those for which it is intended, or by modifications of the instrument not
performed by Uvitec Ltd. The responsibility of Manufacturer is strictly limited to its staff and to its own supplies. In the
case of dispute, only the commercial court of Cambridge (United Kingdom) shall be competent, even in third party
claims proceedings or when there are several co-defendants.

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Safety instruction
Introduction
The UVIDOC imaging system is powered by mains voltage, may be equipped with a UV light source, and is used to
capture images of samples that may be hazardous. Before installing, operating or maintaining the instrument, you must
be aware of the hazards described in the user documentation. Follow the instructions provided to avoid personal injury
or damage to the instrument.
Safety notices
This user documentation contains safety notices (WARNING and UV WARNING) concerning the safe use of the product.
See definitions below.
WARNING
Whenever you find this pictograph, be sure to refer to this Manual.
WARNING indicates an imminently hazardous situation which, if not avoided, could result in death
or serious injury. It is important not to proceed until all stated conditions are met and clearly
understood.
UV WARNING
Whenever you find this pictograph, be sure to refer to this Manual.
UV WARNING indicates an imminently hazardous UV radiation situation which, if not avoided,
could result in serious injury. It is important not to proceed until all stated conditions are met and
clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid damage to the instrument or other
equipment or to ensure the instrument is properly working.

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General precaution
WARNING
Do not operate the UVIDOC imaging system in any other way than described in the User Manual.
WARNING
Use of this instrument in other ways than those specified in the user documentation may result in
physical damage because of exposure to irradiated light or electric shock or injury from touching an
operating part.
WARNING
Do not use the UVIDOC imaging system in dangerous atmosphere or with dangerous materials for
which the UVIDOC imaging system has not been designed for.
WARNING
Do not use the instrument if smoke, atypical noises or odors can be perceived, or if the instrument
becomes unusually hot as this may result in fire or electric shock. Stop using the instrument
immediately, turn off the power switch, and unplug the instrument from the power outlet. Contact
Uvitec Ltd representative to request repair.
WARNING
To prevent fire or shock hazard, do no expose the unit to rain or moisture.
Do not pour liquids directly on or inside the instrument.
Switch off all the lights immediately after use.
Clean the transilluminator platen after use.

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WARNING
Ensure that all the ventilation-opening systems are not obstructed. The obstruction of the air
admission grids may affect the performance of the system and cause operational failure. To ensure
adequate cooling ensure there is at least 40 cm of free space in front of the instrument and at least
10 cm on all other sides to walls or other equipment.
WARNING
The use of accessories not supplied by Uvitec Ltd can damage the system or create safety hazard.
WARNING
When using hazardous chemicals, take all suitable protective measures, such as wearing protective
glasses and gloves resistant to the substances used. Follow local and/or national regulations for
safe operation and maintenance of the system.
WARNING
Do not use the instrument within or near a sink, or in humid (above 70% RH) or dusty
environments. This can result in fire or electric shock.
WARNING
As the equipment is heavy, it cannot be carried by one person. Two service person are necessary
when moving or relocating safely the main body of the equipment. If the equipment drops, it may
be broken, or you may get seriously injured if it drops on your foot.
WARNING
Do not touch the light source in the instrument. The light source may be hot.

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WARNING
• Do not place objects on top of the instrument.
• Use the handle to open or close the instrument door.
• Handle sample trays with care to avoid dropping them.
• Do not leave samples in the instrument after exposure. If left, these may degrade and cause
damage to the instrument.
• Do not disable the interlock of the equipment as UV light can be emitted if the door is opened.
• Do not open the instrument door during exposure. This will stop the exposure and image data will
be corrupted.
• Do not use abrasive cleaning materials for cleaning. This may cause damage to the instrument.
• Do not use excessive amounts of liquids for cleaning
Important UV safety information
UV WARNING
In any UV WARNING situation, the operator should wear appropriate safety glasses or a protective
mask and gloves. UV radiation can be dangerous for unprotected eyes and skin; therefore we
recommend the user to wear UV protective goggles (LP-70) or face-shield (MP-80 or MP-800).
Protect all skin surfaces (including the neck, ears, and hands).
Use of the UV transilluminator acrylic protective screen does not guarantee the user protection
from UV radiation. The use of protective goggles, mask, and/or gloves is strongly recommended
This instrument should be used only by trained personnel who know the health risks associated
with the UV radiation normally associated with this instrument. Users should be trained on the
appropriate personal protection gear for working with UV light to minimize UV exposure.
In its lowered position, the UV-Pad or UV transilluminator’s acrylic shield provides UV protection.
However, it does not provide complete protection to the user. In its raised position, the acrylic
shield does not protect others who are standing in the area around the imager.

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UV WARNING
Do not touch the UV unit after UV exposure. There is a risk of skin burn.
UV WARNING
The instrument is equipped with a safety interlock. If the interlock is out of order or has been
tampered with, UV and visible light may be emitted, which may cause skin burn and impair vision.
Do not open the instrument door during imaging operation.
WARNING: The non-respect of these instructions can cause very serious burns to the userwhen the instrumentisinuse.
WARNING: Ultra Violet light is dangerous for unprotected eyes and skin, therefore the user must wear UV protective
goggles (Ref. LP70) or a face-shield (Ref.MP-80 or MP-800)
SAVE THESE INSTRUCTIONS
AVISO: Durante el uso del instrumento, no seguir las instrucciones puede causar quemaduras graves al usuario.
PRECAUCION: La radiación ultravioleta puede ser peligrosa para los ojos y la piel expuestos sin protección. Para
protegerse, es impérativo usar gafas o una máscara.
CONSERVAR CUIDADOSAMENTE ESTAS INSTRUCCIONES.
AVERTISSEMENT: Lors de l’utilisation de cet appareil, le non-respect des instructions peut provoquer de graves
brûlures à l’utilisateur.
ATTENTION : Le rayonnement ultraviolet est dangereux pour les yeux et la peau exposée sans protection, il est
impératif de porter des lunettes (Réf. LP70) ou un masque de protection (Réf. MP-80 ou MP-800).
CONSERVER SOIGNEUSEMENT CES INSTRUCTIONS
AVVERTIMENTO: Durante l'utilizzo di questo apparecchio, il non rispetto delle istruzioni puo provocare bruciature
gravi all'utilizzatore.
ATTENZIONE: I raggi Ultra Violetti essendo pericolosi per gli occhi e la pelle esposti senza protezione, è obbligatorio
portare gli occhiali (Ref. LP 70) oppure una maschera di protezione (Ref. MP-80 o MP-800).
CONSERVARE CON CURA QUESTE ISTRUZIONI.

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WARNUNG: während der Benutzung dieses Gerätes kann die Missachtung der Anleitung schwere Verbrennungen an
Personen hervorrufen.
ACHTUNG: Ultraviolettes Licht ist gefährlich für ungeschützte Augen und die Haut. Der Benutzer ist daher angehalten,
eine UV-Schutzbrille (Art. LP-70) oder ein Schutzschild (Art. MP-80 oder MP-800) zu tragen.
BEWAHREN SIE DIESE ANLEITUNG SORGFÄLTIG AUF
警告：使用此组件时，若不遵从使用指导将导致严重烧伤！
警告：紫外线对眼和皮肤有害，操作者必须佩戴紫外防护眼镜（货号：LP70）或面罩（货号MP-80 或MP-
800)
请保存使用指导！
警告：機器の使用に際して、説明書の指示に従わない場合、使用者が重大な熱傷を
こうむる場合があります。
警告：紫外線は裸眼や保護されていない皮膚にとって危険です。使用者はUV保護眼鏡 （Ref.LP70）またはフェ
ースシールド（Ref.MP-80またはMP-800）を装着する必要が あります。
将来のためにこれらの説明書を保管しておいてください
Important electrical power safety information
WARNING
Connect the UVIDOC imaging system to an appropriate AC voltage outlet that is properly grounded
and protected by a circuit breaker. Connecting to ground constitutes an obligatory protection.
WARNING
Power down the system and disconnect the AC main from the unit before performing any
disassembly or repair the instrument.
The system must be unplugged from the AC voltage outlet if it is not intended to use it before a
long time.
WARNING
Before plugging or unplugging the camera cables, ensure the system is off and disconnect the
UVIDOC imaging system from the AC voltage outlet.

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WARNING
Do not damage the power supply cord by bending, twisting, heating or allowing them to become
pinned under the equipment. Using damaged power cords could result in fire or electric shock. If
the power supply cords are damaged, contact us for replacements.
Never pull the cable itself. Disconnect the cable only by grasping the plug.
WARNING
Do not block access to the power switch and power cord. The power switch must always be easy to
access. The power cord with plug must always be easy to disconnect.
WARNING
The UVIDOC imaging system has -serviceable fuses which are located on the rear panel and are a
part of the power entry module. See the Maintenance chapter of this guide for information about
replacing the fuses
WARNING
All the equipment connected to this instrument shall be certified according to IEC 60950 standard.
WARNING
In case of thunder, do not touch the power supply plug, as this can result in electric shock.
WARNING
Turn off the power switch before cleaning the inside of the instrument.

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WARNING
Do not turn off power during image acquisition as this can cause loss of data and damage the
instrument. Only turn off power in an emergency situation.
WARNING - Electrical shock hazard.
All instrument repairs or modifications should be performed by service personnel authorized by
Uvitec Ltd. Do not open any covers or replace parts unless specifically stated in the user
documentation.

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UVIDOC HD6 system installation
Unpacking the system
WARNING
The UVIDOC imaging system is powered by mains voltage, may be equipped with a UV light
source, and is used to capture images of samples that may be hazardous. Before installing,
operating or maintaining the instrument, you must be aware of the hazards described in the user
documentation. Follow the instructions provided to avoid personal injury or damage to the
instrument.
Please, open the UVIDOC HD6 box carefully and verify the contents:
UVIDOC HD6 1
Power cable 1
Instruction manual 1
UVITEC-1D software CD-Rom inside the instruction manual 1
Remove carefully each component from the box.
To remove the UVIDOC HD6 from the box, please use the belts which surround the system and designed for this
purpose
Do not handle the system by the camera or the camera support.
Remove their protective plastic cover.
Place the darkroom at its permanent location, the door facing forward. The cabinet has to be placed near the
computer used for image acquisition.
WARNING
It is recommended that the UVIDOC HD6 darkroom be carried by at least two people –one on each
side- holding the instrument from the bottom side. Be sure that the door is properly closed when
carrying the instrument.
The imager weighs 30kg. Using two people, place the imager onto the benchtop given proper
space, electrical and environmental requirements. Remove the outer packaging materials.

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WARNING
Do not connect the power cable to a power source until all connections are made. The power
source has to be grounded and protected by a circuit breaker.
WARNING
The UVIDOC HD6 system is designed to fit a specific voltage. Please, check the voltage to ensure it
corresponds to the UVIDOC HD6 specifications.
WARNING
Please keep an open area of at least 20 cm at the rear of the cabinet to ensure a proper air
circulation for the system. The system should be located in an area free of excessive dust or
moisture, strong magnetic fields or ionising radiation. It is also recommended that the ambient
temperature be stable and within the range of 15°C to 25°C (20°C recommended) and that the
relative humidity not exceed 70%, non-condensing.
WARNING
Ensure that all of the systems ventilation openings are free of interference. Excessive heat build-
up in the instrument may effect performance or cause operational failure.
The UVIDOC HD6 system should be located away from water, solvents, or a corrosive material, on
a bench top that is dry and stable. The system should be placed away from interfering electrical
signals and magnetic fields. A dedicated electrical outlet should be used to eliminate electrical
interference from other instrumentation in your laboratory
WARNING
Do not defeat any instrument interlocks; they are designed to prevent user injury.
It is compulsory to power down the system and disconnect the AC mains from the unit before
performing any disassembly or repair to the system.

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UV WARNING
The use of the UVIDOC HD6 involves ultraviolet (UV) illumination. Proper precautions must be
taken to avoid eye and skin exposure to the UV light. This instrument is meant for use only by
specialised personnel that know the health risks associated with UV radiation and the chemicals
that are normally used with this instrument.
Installing the hardware
Environmental conditions
Placement
Place the UVIDOC HD6 on a level-working surface.
The imager requires a stable laboratory bench or table capable of supporting 100 kg.
The UVIDOC HD6 is intended for indoor use only.
Free space
LCD panel operation free space required: 50 cm
Right, left, rear and top minimum free space required: 10 cm
Other conditions
Do not install the equipment near a window and avoid direct sunlight. We recommend attached
blinds to nearby windows
Do not install the instrument where it may get wet or flooded
Do not install the instrument in a dusty environment
Do not install the instrument in a place constantly or excessively exposed to vibration or impacts
(next to a centrifuge or to a compressor for instance)
Remove all hot or heat source near the UVIDOC HD6, specifically from the air intake.
Do not place objects near the power outlet to ensure easy access to the power cord for
disconnection in case of emergency.
Environmental
conditions
Temperature: 18°C to 25°C
Humidity: 20% to 70% RH (no dew condensation)

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Imager back side
The system power supply and main switch are positioned at the back side of the instrument.
Connect the imager power cord to the power supply plug (1) and plug into a grounded power outlet. A grounded
circuit capable of delivering the appropriate current and voltage is required for installation. Electrical requirements
is located on the imager label on the rear panel of the imager
Switch on the main switch (2) to position 1 –on.
If required, switch off the main switch (2) to position 0 –off.
The main switch provides power to all of the imager components
1
2
Electrical
requirements

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The imager is operated by an integrated computer, which is accessed through the touchscreen. The integrated
computer mother board connectors can be accessed from the back left side of the screen. When delivered to you,
the Uvidoc HD6 camera and the darkroom control board USB connections are already plugged to the build-in
computer mother board.
The camera USB cable is connected to the mother board USB3 port.
4 alternative USB ports could be used. A mouse and a Keyboard could also be connected to these ports.
For network connection, a LAN network port is also available
The USB port of the left side of the instrument (3) is designed to hold your USB key drive for image saving.
The imager on/off switch (4) starts or stops the Uvidoc HD6 and activates the internal computer, touch screen and
thermoelectric camera.
4
3