Vanguard 1400FLi Series User manual

Operation Manual
1400FLi Series
Covering Models:
1482FLi & 1486FLi

2
Introduction
Thank you for purchasing this VanGuard Microscope. With the user in mind, VanGuard Microscopes are built from modern
designs and should provide a lifetime of reliable performance. We recommend you read this entire manual carefully before
setting up and using the instrument.
The 1400FLi Series innity corrected uorescence microscopes
are the agship of the Vanguard microscope line. Combining
top performance with highly-advanced features and optics, the
1400FLi Series models produce uorescence images of the
utmost in clarity. Choose from brighteld or phase contrast/
brighteld/darkeld.
Viewing Head. Trinocular (Seidentopf) heads rotate 360° and are
inclined at 30°. Both models feature interpupillary and dioptric
adjustment. The heads feature a sliding main prism (70/30 split) to
provide full-time imaging when the vertical tube is in use.
Eyepieces. 10X high eyepoint, wideeld with an 18.5mm eld of view.
Nosepiece. Quintuple, reversed, ball-bearing nosepiece with high-
grade lubricant and positive stops. The nosepiece is reversed
(inward-facing) to allow for easier manipulation of slides and to aid
in keeping the objectives clean.
Objectives. Plan achromatic, uorescence objectives come standard
on both models. Model 1482FLi features plan achromatic brighteld
objectives. Model 1486FLi features plan achromatic phase contrast
objectives. All objectives are innity corrected and are light-reective
coated.
Stage. Delivering a high level of uid motion control and longevity,
the stage measures 160mm x 140mm, and features a removable
spring-clip slide holder and a chemical-resistant nish. Motion is
controlled by a right-hand, low-position coaxial control and is driven
by a rack and pinion system.
Focusing Movement. Coaxial, ultra low-position coarse and ne
focus controls feature a 40mm focusing range and are graduated
to 2 microns per division. Fitted with tension adjustment and safety
autostop.
Condenser. Model 1482FLi comes with a 1.25 N.A. Abbe Condenser.
Model 1486FLi comes with a 1.25 N.A. Zernike condenser with phase
annulus rings for 10X/20X, 40X and 100X; also has brighteld and
darkeld stops. All condensers are mounted on a rack and pinion
focusing mechanism and feature spring-loaded centering knobs and
an iris diaphragm.
Lower Illumination. 20W variable quartz halogen light source.
Comes with blue, green (model 1486FLi only), and dispersion lters.
Upper Illumination. Features a 100W HBO mercury light source
mounted to the rear of the microscope and protected by a sturdy,
all-metal housing. The lamp is easily focused and centered with the
front-mounted lamp viewer. External power supply provides constant,
even light and features an electronic timer which automatically logs
the amount of time the mercury lamp is in use.
Fluorescence Filters. Features a 3-position (2 lter positions and 1
OFF position), sliding lter cube assembly. Fluorescence models come
standard with 2 broadcast lters (blue & green). Custom uorescence
lters available (contact us or your dealer for more details).
Base. Stable 225mm x 160mm base tted with anti-skid rubber feet.
Body. Cast-metal ergonomic body with stain-resistant enamel nish.
1400FLi Series Fluorescence Microscopes
Table of Contents
Introduction:....................................................... 2
Parts & Accessories
Included Parts:.................................................... 3
Optional Accessories:......................................... 4
Microscope Diagrams:........................................ 5
Setup
Assembly:......................................................... 7
Aligning the Condenser:....................................... 11
Alignment of Mercury Lamp............................... 10
Aligning the Phase Contrast Annulus Rings...... 12
Using your 1400FLi Series Microscope
Focusing & Mechanical Stage Mechanisms:...... 13
Setting the Up-Stop Mechanism.................... 13
Interpupillary & Diopter Adjustments:................. 14
Oil Immersion Objectives:................................... 14
Using the Camera Port:..................................... 15
External Power Supply........................................ 16
Replacing the Power Supply Fuse..................... 16
Fluorescence Viewing........................................ 17
Maintenance
Replacing the Lower Lamp:............................... 18
Replacing the Microscope Fuse:........................ 18
Replacement Light Bulb/Fuse Information:........ 18
Specications:.................................................. 19
Warranty:............................................................ 20
See warranty details on page 20 for more information.
Warranty card available at:
www.veegee.com/pages/technical-support-service

Parts & Accessories
Included Parts:
Model 1482FLi
Trinocular Head (1 ea.)
Stand (1 ea.)
Brighteld Condenser (1 ea.)
Epi-Illumination Assembly (1 ea.)
External Power Supply (1 ea.)
10X High Eyepoint Eyepieces (2 ea.)
4X Innity Corrected, UV Pass Plan Objective (1 ea.)
10X Innity Corrected, UV Pass Plan Objective (1 ea.)
40X Innity Corrected, UV Pass Plan Objective (1 ea.)
100X Innity Corrected, UV Pass Plan Objective (1 ea.)
UV Shield (1 ea.)
Neutral Filter (1 ea.)
Blue Filter (1 ea.)
External Power Supply Cable (1 ea.)
Power Cord (2 ea.)
100W Mercury Lamp (1 ea.)
Spare 20W Halogen Lamp (1 ea.)
Spare Fuse, 0.25A, 250V (2 ea.) [Microscope]
Spare Fuse, 8A, 250V (2 ea.) [External Power Supply]
Eyecups (2 ea.)
Tension Wrench (1 ea.)
Dust Cover (1 ea.)
Instruction Manual (1 ea.)
Model 1486FLi
Trinocular Head (1 ea.)
Stand (1 ea.)
Phase Contrast/Brighteld/Darkeld Condenser (1 ea.)
Phase Contrast Centering Telescope (1 ea.)
Epi-Illumination Assembly (1 ea.)
External Power Supply (1 ea.)
10X High Eyepoint Eyepieces (2 ea.)
10X Plan Phase Objective (1 ea.)
20X Plan Phase Objective (1 ea.)
40X Plan Phase Objective (1 ea.)
100X Plan Phase Objective (1 ea.)
4X Innity Corrected, UV Pass Plan Objective (1 ea.)
10X Innity Corrected, UV Pass Plan Objective (1 ea.)
40X Innity Corrected, UV Pass Plan Objective (1 ea.)
100X Innity Corrected, UV Pass Plan Objective (1 ea.)
UV Shield (1 ea.)
100W Mercury Lamp (1 ea.)
Halogen Lamp (1 ea.)
Neutral Filter (1 ea.)
Blue Filter (1 ea.)
Green Filter (1 ea.)
External Power Supply Cable (1 ea.)
Power Cord (2 ea.)
Spare Fuse, 0.25A, 250V (2 ea.) [Microscope]
Spare Fuse, 8A, 250V (2 ea.) [External Power Supply]
Eyecups (2 ea.)
Tension Wrench (1 ea.)
Dust Cover (1 ea.)
Instruction Manual (1 ea.)
3

4
Parts & Accessories
For information about parts, accessories, or service -- contact your dealer directly or contact
VanGuard Microscopes at 1-800-423-8842.
Digital Camera Systems:
Part Number: Description:
1400-CDPC-10 USB Color Digital Camera System, 10 Megapixels
1400-CDPC-5 USB Color Digital Camera System, 5 Megapixels
1400-CDPC-3 USB Color Digital Camera System, 3 Megapixels
Other Accessories:
Part Number: Description:
Call for Part Numbers Scale Reticle
Call for Part Numbers Stage Micrometer
Call for Part Numbers Eyepieces (15X, 20X)
Call for Part Numbers Objectives: Plan Achromatic, Brighteld: 4X, 10X, 20X, 40X, 50X, 60X, 100X (oil)
Plan Achromatic, Phase: 10X, 20X, 40X, 100X (oil)
1200-IOG Immersion Oil, Low Viscosity (1/4 oz. Bottle)
Call for Details Custom Fluorescence Filters
Optional Accessories:

1400FLi Series Parts
5
Eyepieces with
Rubber Eyecups
Nosepiece
Objectives
Condenser
Coarse Focus
Control
Substage
Illuminator
Base
Slide Holder
X-Y Mechanical
Stage Controls
Head Set
Screw
(lower)
Camera Port
Mechanical Stage
Head Assembly
Focusing Tension
Control Knob
Prism Slider
Camera Port
Dust Cap
Iris Diaphragm
Adjustment Lever
Substage
Adjustment
Knob
Diopter
Adjustment
Mechanism
Interpupillary
Adjustment
Mechanism
UV Shield Set Screw
Mercury Lamp
Centering Window
Horizon-
tal Lamp
Centering
Knob
Vertical
Lamp
Centering
Knob
Lamp Door
Set Knob
Epi-Illuminator
Housing
Head Set
Screw (upper)
Lamp Focusing
Knob
Illumination
Tube Slider
Fluorescence
Filter Slider
UV Shield Lamp
Access
Door
Phase Annulus Turret
(1486FLi only)
Condenser Centering
Knobs
Stand
Slide Holder Knobs
Fine Focus
Control
Field Iris
Diaphragm
Model 1486FLi Shown
Lamp
Focusing
Set Screw
Annulus Center-
ing Knob (right)
Colored Filter Holder

6
Pictures and descriptions of the external power supply are shown on page 16.
Power Switch
Variable
Lighting
Control
Up-Stop
Mechanism
1400FLi Series Base, Left Side View
Substage
Adjustment
Knob
Power Cord
Jack
1400FLi Series Stand, Rear View
Carrying
Handle
1400FLi Series Parts

7
Setup
Assembly
Figure 1
Figure 2
1. After removing the microscope parts from the protective foam packaging and
checking it over for all components and accessories (a list is provided on page 3),
you can begin assembly.
2. Place the stand on a stable counter top.
3. Place the epi-illuminator housing assembly on top of the stand so that the dovetail
ange slides into place. Secure with the head set screw (see gure 1a).
NOTE: Do not release the epi-illuminator housing assembly until it is rmly
secured with the head set screw.
Figure 3
4. Place the head assembly on top of the epi-illuminator housing assembly so that the
dovetail ange slides into place. Secure with the knurled head set screw (upper)
(see gure 2a).
NOTE: Do not release the head until it is rmly secured with the head set screw
(upper).
NOTE: The head may be installed directly on the stand if the epi-uorescence
illuminator will not be used.
5. Remove the packing cap from the microscope head trinocular port and attach the
camera port to the head assembly by turning in a clockwise direction until tight
(see gure 3a).
The next 4 pages are dedicated to assembling a working microscope. The following section, “Using your 1400FLi Series
Microscope” (starting on page 13) explains the various features of the microscope and how to use them.
[a]
[a]
[a]
All Models

8
6. Attach the UV shield to the bottom front of the epi-illuminator housing assembly
with the UV shield set screw (see gure 4a).
Setup
7. Slide the eyepieces into the eyetubes and attach eyecups (see gure 5).
8. After removing the objectives from their storage containers, individually install
each one into the nosepiece by twisting them clockwise into the threaded holes
of the nosepiece (see gure 6).
9. Raise the substage and stage to their maximum height. Raise the stage via the
coarse/ne focus controls (see gure 7a), and the substage using the substage
adjustment knob (see gure 7b).
10. Loosen the condenser set screw (see gure 7c) enough to allow the neck of the
condenser to slide through the silver ring.
The next 4 steps describe how to install the condenser. With most microscopes the
condenser comes pre-installed. If this is the case skip ahead to step 13.
Assembly (continued)
Figure 4
Figure 5
Figure 6
Figure 7
[a]
[a]
[b]
[c]

9
11. Slide the neck of the condenser up through the silver ring of the condenser mount
until it will go no further (see gure 8). Make sure that the text “NA 1.25” is facing
upward. If the condenser will not slide freely through the silver ring do not force,
simply wiggle the condenser while lightly pushing up. It may be necessary to
loosen the up-stop mechanism in order to gain sufcient substage clearance
(refer to the instructions on page 13).
12. Once the condenser assembly is in place, lower the substage via the substage
adjustment knob and tighten the condenser set screw.
Note: The brighteld condenser (model 1482FLi) is pictured, but the assembly
instructions are identical for the phase contrast condenser (1486FLi).
Setup
Assembly (continued)
13. Remove the epi-illuminator lamp bracket access door (see gure 9a) from the
epi-illuminator housing by loosening the lamp door setscrew (see gure 9b) at
the top of the lamp bracket access door (located on the right side of the housing).
Once loosened, lift the access door up and away from the housing.
14. The epi-illuminator lamp bracket is shipped with a protective, insulating blank
mounted in place of the lamp in the lamp-mounting sockets (see gure 10a). This
must be removed and replaced by a lamp to operate the unit. Loosen the two
lamp mounting screws and slide out the blank.
WARNING: DO NOT open the housing assembly while the illuminator is in opera-
tion or when it is connected to the power supply. When replacing, allow the lamp
to cool for an extended period of time.
15. A 100W HBO Mercury Lamp comes with the unit. Remove the lamp from its
protective packaging.
Figure 11
Figure 8
[a]
Figure 9
Figure 10
[a]
[b]
Note: Do not touch the glass bulb directly, as ngerprints can etch the quartz
housing which decreases the bulb’s life and performance. If contact is made with
the glass bulb, clean with rubbing alcohol and dry thoroughly.
16. The lamp is polarized (one end is larger than the other). Insert the large end through
the exible mounting socket, but do not tighten the set screw (see gure 11).

10
Setup
Assembly (continued)
1. Once the external power supply is on, move the illumination tube slider into the 2nd or 3rd “open” position. Move the
uorescence lter slider into the middle or “0” position.
2. Look into the mercury lamp centering window located just below the eyepieces. If the lamp is centered, a green ball,
slightly smaller in diameter than the viewing window can be observed. If not turn the vertical and horizontal lamp cen-
tering knobs, located on the right side of the epi-illuminator housing, until the green ball is centered. If you see a green
light in the viewing window, use the focusing lever to concentrate the light into a ball.
3. If the lamp cannot be centered, it may have been mounted too far off center. In this case, the lamp must be physically
moved in its mount. Turn off the external power supply and disconnect the power supply cable from the epi-illumina-
tor housing. ALLOW THE LAMP TO FULLY COOL.Open the lamp bracket access door and reposition the lamp as
needed, being careful not to touch the glass envelope of the lamp. Close the lamp bracket access door and reconnect
it to the external power supply with the power supply cable. Repeat the above procedures until the lamp is centered.
4. Once the lamp is centered and focused, these procedures do not have to be repeated until the lamp is replaced (unless
the settings are changed inadvertently).
Alignment of the Mercury Lamp
NOTE: Please see page 16 to familiarize yourself with the operation of the external power supply before turning
the external power supply on.
21. Plug the power supply cable (see gure 13) into the external power supply by lining
up the notch on the plug with the alignment lug located in the socket. Secure the
power supply cable by tightening the threaded silver ring.
22. Connect the female end of the rst power cord (see gure 14) to the rear of the
microscope, and the male end to a suitable power supply.
23. Connect the female end of the second power cord (see gure 14) to the rear of
the power supply, and the male end to a suitable power supply.
24. Turn the substage illuminator on with the power switch located on the lower left
side of the instrument. If the light does not come on, check to see that the variable
lighting control, located next to the power switch is on the highest setting.
17. When the large end is in the exible mounting socket, manipulate the exible
mounting socket to get the small end into the stationary mounting socket (see
gure 12).
18. Push the small end in as far as it will go and then tighten the set screw on the
stationary mounting socket.
19. Adjust the exible mounting socket so that the set screw is over the metal end of
the lamp, then tighten the set screw.
20. Place the entire assembly back inside the epi-illuminator housing and secure it
in place with the lamp door set screw. Make certain there are no gaps between
the lamp bracket access door and the epi-illuminator housing.
Figure 13
Figure 12
Figure 14
All Models