VICI M50 User manual
Valco Instruments Co. Inc.
m6-m50.MicroLynx.indd
Rev. 9/17
M Series Pump
Models M6 and M50
Instruction Manual
For units with MicroLynx-4 controller,
shipped prior to September 2016
Valco Instruments Co. Inc.
800 · 367·8424 sales
713 · 688·9345 tech
713 · 688·8106 fax
VICI AG International
Schenkon, Switzerland
Int + 41 ·41 ·925·6200 phone
Int + 41 ·41 ·925·6201 fax
North America, South America, and Australia/Oceania contact: Europe, Asia, and Africa contact:
®®
Top and side views of MicroLynx-4 controller, shown actual size
The information in this manual has been carefully checked and believed to be accurate; however,
no responsibility is assumed for inaccuracies.
VICI maintains the right to make changes without further notice to products described in this
manual to improve reliability, function, or design. VICI does not assume any liability arising from
the use or application of this product; neither does it convey any license under its patent rights.
and are trademarks of Intelligent Motion Systems, Inc.
Certain copyrighted figures and diagrams used in this manual are used with the permission
of Intelligent Motion Systems, Inc.
VICI does not recommend the use of an M Series pump in life support or applications
where it may directly or indirectly threaten life or injury. See the Warranty and Disclaimer
statement included in the back of this manual.
Table of Contents
Product Description
Operating Principle..........................................................................................1
Software ..........................................................................................................1
Getting Started
Components of the M Series System .............................................................2
PC System Requirements ...............................................................................2
Hardware Installation
Mounting .........................................................................................................4
Connections ....................................................................................................5
Initial Setup
M6-LHS Software Installation ..........................................................................6
COM Port Assignment ....................................................................................6
Initialization .....................................................................................................6
Selecting Pump and Gearbox Values ..............................................................7
Setting the Backlash and Volume/Revolution Values ......................................7
Priming and Testing the Pump
Tubing Connections (M6 shown) ................................................................... 10
Initial Priming ................................................................................................10
Programming Application Methods
Method Screen Functions .............................................................................12
Example Application Methods
1. Prime System ................................................................................... 14
2. Aspirate and Dispense .....................................................................14
3. Dispense Selected Reagents ...........................................................16
Maintenance
Routine Maintenance ....................................................................................17
Routine Cleaning...........................................................................................17
Chemical Compatibility of Wetted Surfaces
Model M6 ......................................................................................................18
Model M50 ....................................................................................................19
Technical Support ................................................................................................ 20
Returning Pumps for Repair ................................................................................20
Operational and Technical Specifications ............................................................21
Appendix A: MicroLYNX Terminal Section
Terminal Programming Mode ........................................................................22
Appendix B: Setup for Multipump Operation
Connections ..................................................................................................24
Setting the Address of each MicroLYNX .......................................................25
Troubleshooting Party Mode Communications ..............................................28
Warranty ..............................................................................................................30
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1
Product Description
The Cheminert®M Series liquid handling pump is a syringe-free pump
capable of delivering a bidirectional flow to six orders of magnitude. It
is a positive displacement pump, which means that it is self-priming and
tolerant of any gas which may find its way into the fluid lines.Since there
is no separate fill cycle, the pump can be operated continuously, and
volumetric capacity is limited only by time.
RS-232 and RS-485 communication protocols are incorporated into the
microprocessor-driven controller. (USB interface requires an adapter.)
The M Series pump is recommended for any liquid handling applications re-
quiring accuracy and precision. It is particularly suited for applications with
a wide range of volumes (which entail laborious syringe changes with other
pumps) and for applications which benefit from the versatility provided when
the pump is coupled with the optional multiposition reagent selection valve.
Operating Principle
At the core of the pump is a rotor which houses four pistons. As the micro-
stepper motor turns the rotor, the pistons float on a stationary cam; at any
given moment, one piston is filling, one is dispensing, and the other two are
in transit between the fill and dispense positions.
Software
The pump comes complete with M6-LHS software, which employs standard
liquid handling terminology and an integrated valve control package in a
familiar Windows format for everyday lab usage. For more advanced control
of system parameters, IMS (Intelligent Motion Systems) software can be
downloaded directly from the IMS website at www.imshome.com.Hardware
installation (pages 4-5) is the same for all users.
2
Getting Started
Components of the M Series System
Check the contents of the packages against Figure 1 to verify that you have
received all of the components. Contact the factory if anything is missing
or damaged. (NOTE: damaged shipments must remain with the original
packaging for freight company inspection.)
Components include:
•Pump
ModelM6: ProductnumberCP2-4841-D
ModelM50: ProductnumberCP3-8182-D
•Motorassembly
ModelM6: ProductnumberCP-DSM
ModelM50: ProductnumberCP-DSM2
•MicroLYNXcontroller:ProductnumberCP-CM1-P
•Controllermotorpowercablewith5pinDINconnector
•ControllerRS-232ribboncable
•24VDCpowersupply
•110/220VACcableforpowersupply
•Fittings(2)
ModelM6: ProductnumbersZN1FPK(nut)andZGF1PK(ferrule)
ModelM50: ProductnumbersCFL-2BK-S(nut)and
CFL-CB2KF-S(bushing)
•M6-LHSsoftwareCD
•Manual
•Qualitycontrolcerticate
PC System Requirements
PentiumPC100MHzorhigher,runningWindows95,98,ME,NT,2000,
orXP.Thepumpcontrollerrequiresadedicatedserialport.Ifyourpump
systemincludesavalvewithelectricactuator,anadditionalserialportis
required.Alternately,aUSBtoRS-232adapter(notsupplied)canbeused
foroneorbothconnections.Theadaptermaybepurchasedfromany
computer supply outlet.
3
Figure 1: Components of the M Series pump system
4
Hardware Installation
Mounting
The mounting points of the pump motor and controller are given in Figure 2,
along with the external dimensions of each module.
For best results, the M Series pump must be oriented
with its ports facing up. If the pump is oriented in any
other direction, bubbles may be trapped in the internal
chambers of the pump.
The controller should be mounted in such a way to allow adequate cooling.
If the cooling fan is obstructed the controller may overheat.
Figure 2: Mounting points and external dimensions
for the pump, motor, and controller
5
Connections
CAUTION: Supply power to the power supply only after
all connections have been made. Failure to do so may
destroy the controller. Take precautions to avoid static
discharges to the controller.
1. Connectthepowersupplyandmotorpowercabletothecontrolleras
shown below.
2. Connect the motor power cable to the pump.
3. PlugoneendoftheRS-232cableintothepumpcontrollerandtheother
into a serial port on the computer.
4. If the system includes a valve, plug one end of its RS-232 cable into the
valve actuator controller and the other end into a second serial port on
the computer. If a second serial port is not available, an RS-232 to USB
adapter is required.
5. Plugthevalvecontrollerpowersupplyintoa110or220VACsource.
Figure 3: M Series controller cabling diagram with RS-232 connection
6
Initial Setup
M6-LHS Software Installation
InserttheM6-LHSsoftwareCDintothePC.Iftheinstallationdoesn’tbegin
automatically,browsetheCDanddoubleclickonthelecalledsetup.exe.
The installer will place an icon for M6-LHS on the Windows desktop.
COM Port Assignment
1. DoubleclicktheicononthedesktoptostarttheM6-LHSsoftware.
2. On main menu, choose Utility > Setup, as in
Figure 4.
3. Select the correct COM port for the pump and
valve. If no valve is being used, select None.
(Figure 5)
4. Click Apply, then Close.
Figure 4: Select “Setup”
Initialization
1. Onthemainmenu,chooseUtility>InitializePump.Ifinitializationis
successful, proceed to the next section, “Setting the Backlash and
Volume/RevolutionValues”.
2. If the error message in
Figure 6 appears, close the
window, then check that all
the connectors are com-
pletely plugged in and make
sure all the dip switches on
the controller are in the “up”,
or OFF position. Repeat
Step1.
3. Ifinitializationissuccessful,proceedtothenextsection,“Settingthe
BacklashandVolume/RevolutionValues”.
4. If the error message persists, make sure that the COM port assignments
correspond to the ports to which the cables are physically connected.
RepeatStep1.
Figure 5: Select COM ports for pump and valve
ASSIGNCOMPORT
VALUESFORPUMP
ANDVALVE
Figure 6:Initializationerrormessage
7
Selecting Pump and Gearbox Values
In the Gearbox pulldown menu, select CP-DSM1 or CP-DSM2, based on
the gearbox type indicated on the motor label. If the label says CP-DSM,
select CP-DSM1.
In the Pump pulldown menu, select 100 for Model M6 or 625 for Model M50.
(The values 4 and 750 are currently unused.) These values represent
nominal µL/revolution for the two models. The next section discusses how
these can be overridden for greater accuracy.
Setting the Backlash and Volume/Revolution Values
Backlash is defined as the transition from the aspirate function to the
dispense function, or vice versa. The MicroLYNX software observes the
factory default settings of 1.5 µL for backlash, and 100 µL or 625 µL for
volume/revolution (as set above, depending on the pump model).
However, the actual values for each pump are calibrated and recorded on
the QC certificate supplied with every unit. There are several situations,
delineated below, in which these values must be entered manually to
override the factory defaults.
Manual entry of backlash and µL/rev values is required:
(Refer to page 23.)
(If it says CP-DSM, select CP-DSM1.)
Figure 7: Selecting pump and gearbox values
8
Figure 8: Setting the backlash and volume per revolution values
CLICKTHEBOXANDENTERTHE
µL/REVFROMTHEQCCERTIFICATE
CLICKTHEBOXANDENTERTHE
BACKLASHVALUEFROMTHE
QCCERTIFICATE
To override the factory default values for backlash and µL/rev:
1. LocatethebacklashandµL/revvaluesontheQCcerticate.
2. Click the Enable box under Backlash on the Setup screen, and type in
the backlash value.
3. ClicktheUsemeasureduL/revboxunderPump,andtypeinthevolume
per revolution.
4. Click Apply, then Close.
Initial Setup
9
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10
Priming and Testing the Pump
At this stage, some basic tests will determine that the tubing connections are
tight and that the pump is rotating in the proper direction. The tests include an
initial priming of the pump to remove all air and unwanted solutions in the tubing.
Use proper safety precautions when pumping flammable
liquids. In all applications, make sure you have enough
tubing and sufficient quantities of solutions ready for
use before beginning a procedure.
Tubing Connections (M6 shown)
M6pumpsshouldnotbeusedwithtubingODssmallerthan1/16".ForM50
pumps,theminimumODis1/8".
Improperly installed fittings can create space for a
bubble to become trapped, degrading pump performance.
The use of non-VICI fittings can also adversely affect
pump function and performance.
1. Connectalengthoftubingtotheinlet(PortA)andplacetheotherendin
a vessel containing isopropyl alcohol.
2. Connectasecondlengthoftubingtotheoutlet(PortB)andplacethe
other end into a receiver bottle.
Fitting Assembly Instructions
1. Slidethenutandferruleonto
the tubing in the order shown.
2. Insert this assembly into the
fitting detail, screwing the nut in
two or three turns by hand.
3. Pushthetubingallthewayforwardintothedetailsothatitseatsrmly.
This is essential for a proper zero dead volume connection.
4. Turn the nut into the detail until it is finger tight.
Initial Priming
In the Test Mode, a mouse click on a button directs the pump to either
aspirate or dispense. (Refer to page 13, No. 7 for a definition, if required.)
WhentheEnableboxintheFixedVolumeareaischecked,(seeFigure 8)
thepumpwillaspirateordispensethequantityenteredintheFixedVolume
field and then stop. If the Enable box is not selected, the pump will continue
to run at the set Flow Rate until either Stop (stops aspirating or dispensing)
orQuit(exitstheTestMode)isselected.
If the pump aspirates a volume larger than the outlet
tube volume, sample or reagent could enter the pump
and mix with liquid in the reservoir.
11
1. IfthescreeninFigure 9 is not already open, go to the main menu, and
choose Utility > Test.
2. IfthetubingIDis0.010”orless,enter
FlowRate: 200µL/min
FixedVolume: 1000µL
Click the Enable box
IfthetubingIDislargerthan0.010”,enter
FlowRate: 9000µL/min
FixedVolume: 1000µL
Click the Enable box
(In the Test Mode, user-entered flow parameters cannot be saved;when you
exit the program, these values will revert to defaults.)
NOTE:SmallerIDandlongerlengthtubingcancauseexcessive
restriction. Adjust flow rates accordingly. Maximum backpressure
is100psi.
3. ClicktheDispensebutton.Thedesignatedvolumeofisopropylalcohol
should move through the tubing and into the receiver bottle, removing
any air bubbles and priming the system. If it does, the pump is primed
and ready for use.
NOTE: If a buffer will be used, flush all the isopropyl alcohol out of
the lines by placing the inlet tube in a vessel of water, changing the
xedvolumeto2000µL,andclickingtheDispensebutton.
4. If the pump doesn’t seem to draw any isopropyl alcohol into the tubing:
(a) Check for leaks around the fittings and make sure the tubing is well
seated in the pump head.
(b) Make sure the end of the tubing is free to draw liquid into the pump.
(TIP:Cutthetipendthatgoesintotheisopropyl
alcohol reservoir at a 45° angle.)
(c) Touch the pump to make sure it is on.
Ifthepumpstilldoesn’tseemtofunctionproperly,consultthefactory.
Figure 9:PrimingthepumpintheTestMode(M6valuesshown)
CLICKTHEBOX
FOR A ONE TIME
FIXEDVOLUME
ASPIRATEOR
DISPENSE.
LEAVETHEBOX
UNCLICKEDFOR
CONTINUOUS
PUMPINGATTHE
ENTEREDFLOW
RATE.
12
Figure 10: The Method screen
Programming Application Methods
Method Screen Functions
From the main menu, choose Method > New to open the screen below.
1: Method status
Idle, Active (running), or Editing
2: Mode activation buttons
Run: Starts running the method.
Reset: Pausesamethodwhichisunderway,allowingachangetothe
editing mode.
Repeat: If this box is clicked, when the method completes the last
action, it goes back to first action in a continuous loop.
3: Function status
Indicates the current activity during a run.
4: Event button
Refer to No. 11: Event box.
5: Edit and Close buttons
Edit: When the method is idled, clicking this button changes it into
the editing mode.
Close: Closes the method screen.
13
6: Action line
A step in the method sequence, reflecting the values entered by selecting
ortypingvaluesinthePump,Valve,andWaitelds.Tomovetoadiffer-
ent line for editing, just click on it or use No. 12: Up and Dn buttons.
7: Pump function
Aspirate: Drawliquidinto the line connected to the pump outlet(PortB).
Dispense: Force a pre-programmed amount of liquid through the pump.
Wash: Wash or flush the lines (always in the outward direction,
or A to B) with system fluid or a pre-programmed reagent
selected.
8: Volume
The amount to be aspirated, dispensed, or sent through as a wash.
9: Flow rate
The rate at which the given volume is aspirated, dispensed, or sent
through as a wash.
10: Wait field
If a value is entered in this field for an action line, during a run the
program will wait for the designated time (in seconds) after the
completion of that action before advancing to the next action. Used
after an aspirate or dispense step to allow the liquid to equilibrate in the
line or the dispensed liquid to come to equilibrium, as with viscous
liquids or very small capillary tubing.
11: Event box
When this box is clicked when programming an action line, the program
will stop before that action is executed. The program will remain stopped
until No. 4: Event Button is clicked. (If the step before the event line
has a programmed Wait interval, the Event button will not trigger the
action until the Wait interval has elapsed.)
12: Up and D(ow)n buttons
In the Editing mode, these move the line highlighter up and down.
13: Valve type
Multi-Pos: Multipositionstreamselector
2-Pos: Twopositionswitchingvalve/injector
14: Valve Position
The position the valve needs to be in to select the proper solvent or
reagent.
15: Valve Direction(forMulti-Posonly)
The direction the valve will take to the indicated position – the shortest
(used in most cases), clockwise, or counterclockwise.
16: Insert button
Inserts a new action line above the highlighted line.
17: Duplicate button
Duplicatesthehighlightedline.Thenewlinecanthenbeeditedasrequired.
18: Delete button
Deletesthehighlightedline.
19: End Edit button
When editing is complete, click this button to return to the Idle mode,
ready to Run.
14
Example Application Methods
In the Test Mode, user-entered flow parameters cannot be saved;when you
exit the software, the values revert to defaults. So creating several simple
example methods is a useful way to start learning the M6-LHS software.
ThemethodsbelowutilizeowparameterscharacteristicoftheModelM6,
butthesameprinciplesapplytoprogrammingfortheM50.
Example method 1: “Prime System”
1. Fromthemainmenu,chooseMethod>Newtoopenthescreenin
Figure 10onpage12.
2. Action line one will be highlighted. In the pump function field, select
Dispense,andenterthevolumeandowratevaluesfromStep2atthe
topofpage11.Thiswilldispensetheisopropylalcohol,asinourinitial
priming done in the Test mode.
3. ClickDuplicatetoaddanew,identicalaction.
4. While action line 2 is highlighted, check the Event box.
5. Click the End Edit button.
6. On the main menu, choose Method > Save, and call this method “prime
system”.
Running “prime system”
1. Withtheinlettubeinavesselofisopropylalcohol,clicktheRunbutton.
TheboxnexttoDispenseinthefunctionstatusareawillturngreen,indi-
cating that the first action line is underway.
Since the Event box for action line 2 is checked, the program will pause
before executing action line 2.
2. Move the inlet tube to a vessel of water and click on the Event button to
start action line 2, which now dispenses water to flush the alcohol from
the system.
Example method 2: “Aspirate and Dispense”
1. Fromthemainmenu,chooseMethod>Newtoopenthescreenin
Figure 10onpage12.
2. Action line one will be highlighted. In the pump function field, select
Wash,thenenter1000forthevolumeand9000fortheowrate.For
larger tubing, increase the wash volume to equal five times the tubing
volume.
3. ClicktheDuplicatebuttontomakeanidenticalWashactionline.
4. UsetheDn(down)buttontohighlightthesecondline,thenclickthe
Insert button.
5. For the newly inserted line, select Aspirate in the pump function field,
thenenter10forthevolumeand100fortheowrate.
6. ClicktheDuplicatebuttontomakeanidenticalAspirateline.
7. Click on the newly created line to highlight it, then change the volume to
100andtheowrateto500,andenter“2”intheWaitbox.
Programming Application Methods
15
8. UsetheDn(down)buttontohighlightthelastline,thenclicktheInsert
button.
9. Forthenewlyinsertedline,selectDispenseinthepumpfunctioneld,
thenenter110forthevolumeand1000fortheowrate.
10. ClicktheEndEditbutton.
11. Onthemainmenu,chooseMethod>Save,andcallthismethod
“aspirate and dispense”.
The result should look like Figure 11.
TIP
The method in Figure 11 is written so all functions will be
automatically performed when the Run button is clicked.
For manual operation, check the Event box for each action line.
Then each line is performed by clicking the Event button.
Figure 11:Example“AspirateandDispense” method for Model M6
Programming Application Methods
16
Figure 12:Methodscreen(partial)forexample“DispenseSelectedReagents”method for Model M6
Example method 3: “Dispense Selected Reagents”
This method, which employs an 8-position multiposition valve for selecting
multiple reagents to be added to tubes or microplates, is a typical example
oftheapplicationspossiblewhenaValcoorCheminertvalveisintegrated
into the pump/M6-LHS system. A wash step (or in this case, a prime step)
is incorporated between reagents.
NOTE: Wash is always in the dispense or outward direction of the
pump, and can be used for washing with a system fluid, or, as in
this case, a reagent.
By now you should know how to enter all the information shown in Figure 12
to create this method. (Only the first four positions for the 8-position valve
are shown.)
TIP
YoumightndithandytousetheDuplicatebuttontocreateall
thenearlyidenticalMultiValveactionlines,thengobackwiththe
InsertbuttontocreatetheWashandDispenselines.
Programming Application Methods
This manual suits for next models
2
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