Agilent Technologies InfinityLab LC Series User manual

Agilent InfinityLab LC Series
1260 Infinity II Binary Pump
User Manual
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual
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Document No: SD-29000214 Rev. C
Edition: 10/2021
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InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 3
In This Guide...
This manual covers the Agilent InfinityLab LC Series 1260 Infinity II Binary Pump
(G7112B).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information about site requirements and specifications for
the binary pump.
3UsingthePump
This chapter explains the operational parameters of the Binary Pump.
4 Optimizing Performance
This chapter gives information on how to optimize the performance of the Binary
Pump under special operational conditions.
5Troubleshootingand Diagnostics
Overview of the troubleshooting and diagnostic features.
6 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
7 Test Functions and Calibration
This chapter explains all test functions that are available for the binary pump.
8 Maintenance
This chapter describes the maintenance of the module.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 4
9 Parts and Materials for Maintenance
This chapter lists all parts and tools that are required for maintenance and simple
repairs.
10 Identifying Cables
This chapter provides information on cables used with the Agilent InfinityLab LC
Series modules.
11 Hardware Information
This chapter provides detailed technical information about your binary pump.
12 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
13 Appendix
This chapter provides addition information on safety, legal and web.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 5
Contents
1Introduction10
Instrument and Operation 11
Overview of the Hydraulic Path 15
Leak and Waste Handling 20
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
3UsingthePump32
Magnets 33
Turn on/off 34
Status Indicators 36
Best Practices 37
Leak and Waste Handling 39
Hints for Successful Use of the Binary Pump 40
Setting up the Pump with the G4208A Instant Pilot 41
Setting up the Pump with the Instrument Control Interface 42
Solvent Information 47
Algae Growth in HPLC Systems 54
Prevent Blocking of Solvent Filters 55
Normal Phase Applications 57
4 Optimizing Performance 59
When to Use a Vacuum Degasser 60
When to Use the Active Seal Wash Option 61
When to Use the Low Volume Mixer 62
When to Remove Damper and Mixer 63
How to Optimize the Compressibility Compensation Setting 65

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 6
5 Troubleshooting and Diagnostics 68
Overview of the Module’s Indicators and Test Functions 69
User Interfaces 71
Agilent Lab Advisor Software 72
6 Error Information 73
What Are Error Messages 74
General Error Messages 75
Module Error Messages 81
7 Test Functions and Calibration 92
(System) Pressure Test 93
Valve Test 98
Binary Pump Solvent Compressibility Calibration 100
Pump Elasticity Calibration 102
Pump Leak Rate Test 104

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 7
8 Maintenance 108
Introduction to Maintenance 109
Cautions and Warnings 110
Cleaning the Module 112
Overview of Maintenance and Simple Repairs 113
Maintenance Procedures 114
Remove and Install Doors 115
Exchange the Purge Valve Frit or the Purge Valve 117
Replace the O-Ring on the Purge Valve 120
Remove the Pump Head Assembly 122
Maintenance of a Pump Head without Seal Wash 124
Maintenance of a Pump Head with Seal Wash 127
Reinstall the Pump Head Assembly 131
Seal Wear-in Procedure 133
Exchange the Active Inlet Valve (AIV) or its Cartridge 134
Exchange the Seal Wash Cartridge 137
Replace Leak Handling System Parts 139
Exchange the Outlet Valve 141
Installation of the Solvent Selection Valve Upgrade Kit 143
Exchange the Solvent Selection Valve 145
Replacing Module Firmware 148

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 8
9 Parts and Materials for Maintenance 149
Hydraulic Path with Solvent Selection Valve 150
Hydraulic Path without Solvent Selection Valve 152
Pump Head Assembly Without Seal Wash 154
Pump Head Assembly with Seal Wash Option 156
Outlet Valve 158
Purge Valve Assembly 159
Active Inlet Valve Assembly 160
HPLC System Tool Kit 161
Active Seal Wash Option 162
Solvent Cabinet 163
Bottle Head Assembly 164
Accessory Kit 165
Cover Parts 166
10 Identifying Cables 167
Cable Overview 168
Analog Cables 170
Remote Cables 172
CAN/LAN Cables 176
RS-232 Cable Kit 177
Agilent 1200 Module to Printer 178
11 Hardware Information 179
Firmware Description 180
Electrical Connections 183
Interfaces 185
Setting the 6-bit Configuration Switch 193
Early Maintenance Feedback 197
Instrument Layout 198

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 9
12 LAN Configuration 199
What You Have to Do First 200
TCP/IP parameter configuration 201
Configuration Switches 202
Initialization Mode Selection 203
Dynamic Host Configuration Protocol (DHCP) 205
Manual Configuration 208
13 Appendix 212
General Safety Information 213
Waste Electrical and Electronic Equipment (WEEE) Directive 219
Radio Interference 220
Sound Emission 221
Agilent Technologies on the Internet 222

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 10
1Introduction
Instrument and Operation 11
Introduction to the Pump 11
Principle of Operation 12
Product Description 14
Features 14
Overview of the Hydraulic Path 15
Leak and Waste Handling 20
Leak Sensor 23
Waste Concept 24
This chapter gives an introduction to the module and an instrument overview.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 11
1Introduction
Instrument and Operation
Instrument and Operation
Introduction to the Pump
The binary pump comprises two identical pumps integrated into one housing.
Binary gradients are created by high-pressure mixing. A built-in degasser is
available for applications that require best flow stability, especially at low flow
rates, for maximum detector sensitivity. Pulse damper and mixer can be
bypassed for low flowrate applications or whenever a minimal transient volume
is desirable. Typical applications are high throughput methods with fast
gradients on high resolution 2.1 mm columns. The pump is capable of delivering
flow in the range of 0.1 – 5 mL/min against up to 600 bar. A solvent selection
valve (optional) allows to form binary mixtures (isocratic or gradient) from one of
two solvents per channel. Active seal wash (optional) is available for use with
concentrated buffer solutions.
Figure 1 Overview of the binary pump

1Introduction
Instrument and Operation
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 12
Principle of Operation
The binary pump is based on a two-channel, dual-piston in-series design which
comprises all essential functions that a solvent delivery system has to fulfill.
Metering of solvent and delivery to the high-pressure side are performed by two
pump assemblies which can generate pressure up to 600 bar.
Each channel comprises a pump assembly including pump drive, pump head,
active inlet valve with replaceable cartridge, and outlet valve. The two channels
are fed into a low-volume mixing chamber which is connected via a restriction
capillary coil to a damping unit and a mixer. A pressure sensor monitors the
pump pressure. A purge valve with integrated PTFE frit is fitted to the pump
outlet for convenient priming of the pumping system.
Figure 2 The hydraulic path of the Binary Pump with damper and mixer
SSV
Seal wash pump
Pump head A Pump head B
Damper
Mixer
Pressure sensor
Mixing chamber
Inlet valve Inlet valve
Outlet valve Outlet valve
to waste
to sampler
Purge valve

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 13
1Introduction
Instrument and Operation
Damper and mixer can be bypassed for lowest delay volume of the binary pump.
This configuration is recommended for low flow rate applications with steep
gradients.
Figure 3 on page 13 illustrates the flow path in low delay volume mode. For
instructions on how to change between the two configurations, see “Convert the
Binary Pump to Low Delay Volume Mode” on page 64.
Figure 3 The hydraulic path of the Binary Pump with bypassed damper and mixer
For pump specifications, see “Performance Specifications” on page 30.
NOTE
Bypassing the mixer while the damper remains in line is not a supported
configuration and may lead to undesired behavior of the binary pump.
SSV
Seal wash pump
Pump head A Pump head B
Damper
Mixer
Pressure sensor
Mixing chamber
Inlet valve Inlet valve
Outlet valve Outlet valve
Purge valve
to waste
to sampler

1Introduction
Instrument and Operation
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 14
Product Description
The Agilent 1260 Infinity II Binary Pump is suited for LC applications using
conventional or superficially porous particles columns, as well as STM column
technology. Gradient formation is based on a high-pressure mixing principle.
Standard or low delay volumes can be easily configured by the user. The Agilent
1260 Infinity II Binary Pump offers reproducible gradients and high-performance,
providing high-throughput and fast separations. The pump is suitable for routine
applications, with UV or MS detection, where high speed and resolution with
uncompromised data quality are required.
Figure 4 Overview of the pump
Features
•Configurable delay volume - down to 120 µL together with a flow range up to
5 mL/min provides universal applicability.
•Change from standard to low delay volume configuration is enabled.
•High gradient performance - even at low % B and narrow-bore flow rates.
•Integrated 2-channel-degasser.
•Fast and precise gradients using LC/MS, as well as UV-only systems.
•Fully exploits the speed and separation potential of Poroshell.
Status indicator
Active seal wash
Purge valve
Degasser
Pump head A
Pump head B
Leak drain
Power switch

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 15
1Introduction
Overview of the Hydraulic Path
Overview of the Hydraulic Path
The solvent from the bottle in the solvent cabinet enters the pump through an
active inlet valve. Each side of the binary pump comprises two substantially
identical pump units. Both pump units comprise a ball-screw drive and a pump
head with two sapphire pistons for reciprocating movement.
Figure 5 Pump head
A servo-controlled variable reluctance motor drives the two ball-screw drives in
opposite directions. The gears for the ball-screw drives have different
circumferences (ratio 2:1) allowing the first piston to move at double the speed of
the second piston. The solvent enters the pump heads close to the bottom limit
and leaves it at its top. The outer diameter of the piston is smaller than the inner
diameter of the pump-head chamber allowing the solvent to fill the gap in
between. The first piston has a stroke volume in the range of 20 µL to 100 µL
depending on the flow rate. The microprocessor controls all flow rates in a range
Inlet valve
From solvent bottle
To mixing chamber
Outlet valve
Seal
Piston
/degasser/SSV

1Introduction
Overview of the Hydraulic Path
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 16
of 1 µL/min to 5 mL/min. The inlet of the first pumping unit is connected to the
active inlet valve which is processor-controlled opened or closed allowing solvent
to be drawn into the first pump unit.
The outlet of the first pump chamber is connected by a 500 µL absorber capillary
to the second pump chamber. The outlets of the second chambers of both pump
channels joined via a small mixing chamber. A coiled restriction capillary
connects the mixing chamber via a pressure pulse damper, a mixer and a
pressure sensor to the purge valve assembly. The outlet of the purge valve
assembly is then connected to the attached chromatographic system.
When turned on, the pump runs through an initialization procedure to determine
the upper dead center of the first piston of both pump channels. The first piston
moves slowly upwards to the mechanical stop of the pump head and from there
it moves back a predetermined path length. The controller stores this piston
position in memory. After this initialization the pump starts operation with the set
parameters for the two pump channels.
The active inlet valve is opened and the down moving piston draws solvent into
the first pump head. At the same time the second piston is moving upwards
delivering into the system. After a controller defined stroke length (depending on
the flow rate) the drive motors are stopped and the active inlet valve is closed.
The motor direction is reversed and moves the first piston up until it reaches the
stored upper limit and at the same time moving the second piston downwards.
Then the sequence starts again moving the pistons up and down between the
two limits. During the delivery stroke of the first piston the solvent in the pump
head is pressed through the outlet valve into the second pumping unit. The
second piston draws in half of the volume displaced by the first piston and the
remaining half volume is directly delivered into the system. During the drawing
stroke of the first piston, the second piston delivers the drawn volume into the
system.
For pump specifications, see “Performance Specifications” on page 30.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 17
1Introduction
Overview of the Hydraulic Path
What is Pump Elasticity Compensation?
The flow path of the pump consists of pump chambers, sapphire pistons,
polymer seals, stainless steel tubing of different dimension, pressure sensor, and
so forth. All of these parts deform when pressurized. The sum of this
deformation is called pump elasticity.
Let us look at a practical example: Piston 1 draws solvent at ambient pressure.
The movement direction is reversed and the piston 1 now compresses the
solvent until the operating pressure of the HPLC system is reached. The outlet
valve opens, and solvent is pumped by piston 1 into pump chamber 2. Due to two
factors, the solvent volume that is delivered into the system at high pressure is
smaller than it is supposed to be:
1The solvent is compressible
2The pump has a certain elasticity which causes its internal volume to increase
with pressure.
In order to compensate for these two influences, their contributions must be
known. An elasticity calibration allows separating pump properties from solvent
properties and therefore allows transferring solvent properties, which have been
obtained from one pump to another pump with different elasticity.
Elasticity calibration is done with a solvent, which properties (compressibility,
thermal expansion) are well-known and documented: pure water. When pumping
water and using its property data for controlling the pump, any deviations from
the theoretical pressure profile during solvent recompression are caused by the
elasticity of the pump.
The Pump Elasticity Calibration calculates correction factors to compensate for
the individual elasticity of the pump that is being calibrated. The elasticity is
different for every pump and may change with the replacement of parts in the
flow path, e.g. pump seals.
All binary pumps are elasticity calibrated at the factory and require recalibration
only after preventive maintenance or major repairs to the flow path. Replacement
of capillaries or PTFE frits are not considered as a major repair.
CAUTION
Incorrect pump elasticity calibration.
Solvent compressibility calibrations acquired with a miscalibrated pump will
work, but they are not transferable to other pumps. A correct pump elasticity
calibration is an essential prerequisite for successful solvent compressibility
calibrations.
Calibrate the pump elasticity correctly.

1Introduction
Overview of the Hydraulic Path
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 18
What is Solvent Compressibility Compensation?
Although the compressibility of liquids is orders of magnitude lower than the
compressibility of gases, without correction a noticeable volume error would be
seen if typical chromatographic solvents are compressed to operating pressures
as high as 600 bar. In addition, the compressibility depends on pressure,
temperature and the amount of dissolved gas. In order to minimize the influence
of the latter, the use of a vacuum degasser is mandatory for a high flow and
composition precision. Unfortunately, the influence of the temperature on
compressibility is non-linear and cannot be calculated.
The Agilent 1260 Infinity II Binary Pump features a multi point compressibility
calibration. The compressibility of a solvent is determined at different pressures
from 0 – 600 bar and stored in an XML file. This file can be distributed to other
pumps because the solvent compressibility is independent from the pump.
The binary pump and ChemStation come with predetermined solvent
compressibility data for the most common HPLC solvents like water, acetonitrile,
methanol, etc. Users can calibrate their own solvent mixtures with the help of an
easy to use calibration procedure in the Agilent Lab Advisor software.
Let us use the practical example from the last section once again to understand
how compressibility compensation works:
Piston 1 draws solvent at ambient pressure. The movement direction is reversed
and piston 1 now compresses the solvent until the operating pressure of the
HPLC system is reached. The outlet valve opens, and solvent is pumped by
piston 1 into pump chamber 2.
Without any compensation, the delivered volume at operating pressure would be
too low. In addition, it would take a noticeable amount of time to recompress the
solvent to operating pressure. During this time frame, no solvent would be
delivered into the system and as a result a high pressure fluctuation (known as
pressure ripple) would be observed.
When both solvent compressibility at the current operating pressure and pump
elasticity are known, the pump can automatically correct for the missing volume
by drawing the appropriate larger solvent volume at ambient pressure and speed
up the piston during the recompression phase in the first pump chamber. As a
result, the pump delivers the accurate volume with any (calibrated) solvent at any
pressure at a greatly reduced pressure ripple. For applications that require lowest
transition volume of the pump, damper and mixer can be bypassed.
For compatibility with older methods from G1312A Binary Pumps, the previous
one-point compressibility compensation is available, too. However, since the
compressibility is a non-linear function, one single compressibility value per
solvent will only give good results at one particular pressure.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 19
1Introduction
Overview of the Hydraulic Path
How Does Variable Stroke Volume Work?
The smaller the solvent volume in the pump chamber is, the faster it can be
recompressed to operating pressure. The binary pump allows to manually or
automatically adjust the pump stroke volume of the first piston in the range of
20 – 100 µL. Due to the compression of the solvent volume in the first pump
chamber, each piston stroke of the pump will generate a small pressure
pulsation, influencing the flow ripple of the pump. The amplitude of the pressure
pulsation mainly depends on the stroke volume and the compressibility
compensation for the solvent in use. Small stroke volumes generate less
pressure pulsation than larger stroke volumes at the same flow rate. In addition,
the frequency of the pressure pulsation will be higher. This will decrease the
influence of flow pulsations on retention times.
In gradient mode, a smaller stroke volume results in less flow ripple and reduces
the composition ripple.
The binary pump uses a processor-controlled ball screw system for driving its
pistons. The normal stroke volume is optimized for the selected flow rate. Small
flow rates use a small stroke volume while higher flow rates use a higher stroke
volume.
The stroke volume for the pump is by default set to AUTO mode. This means that
the stroke is optimized for the flow rate in use. A change to larger stroke volumes
is possible but not recommended.

1Introduction
Leak and Waste Handling
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 20
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler or Sample Thermostat (condensate)
•from the pump's Seal Wash Sensor (if applicable)
•from the pump's Purge Valve or Multipurpose Valve