VWR International 2310 User manual

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WATER JACKETED CO2
INCUBATORS
WITH MICROPROCESSOR CONTROL
MODEL: 2310, 2300, 2350
2400, 2450
INSTALLATION AND OPERATION MANUAL
Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113
EMAIL: [email protected] INTERNET: http://www.Shellab.com/~Shellab
1-800-322-4897 [phone] FAX [phone]
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TABLE OF CONTENTS
SECTION 1.0 RECEIVING AND INSPECTION
SECTION 2.0 GRAPHIC SYMBOLS
SECTION 3.0 INSTALLATION
SECTION 4.0 CONTROL PANEL OVERVIEW
SECTION 5.0 OPERATION
SECTION 6.0 FYRITE CO2CHECKING
SECTION 7.0 MAINTENANCE
SECTION 8.0 TROUBLESHOOTING
SECTION 9.0 PARTS LIST
UNIT SPECIFICATION
SCHEMATICS
REV 02/07
4861519
These units are CO2Water Jacketed incubators for professional, industrial or educational
use where the preparation or testing of materials is done at approximately atmospheric
pressure and no flammable, volatile, or combustible materials are being heated. These
units are not intended for hazardous or household locations or use.
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INTRODUCTION
Thank you for choosing a water jacket incubator. These units are not intended for use at
hazardous or household locations.
Before you use the unit, read this entire manual carefully to understand how to install,
operate, and maintain the unit in a safe manner. Your satisfaction with the unit will be
maximized as you read about its safety and operational features.
Keep this manual on-hand so it can be used by all operators of the unit. Be sure all
operators of the unit are given appropriate training before you put the unit in service.
Note: Use the unit only in the way described in this manual. Failure to follow the
guidelines and instructions in this manual may be dangerous and illegal.
General Safety Considerations
Your incubator and its recommended accessories have been designed and tested to
meet strict safety requirements.
For continued safe operation of your incubator, always follow basic safety precautions
including:
•Read this entire manual before using the incubator.
•Be sure you follow any city, county, or other ordinances in your area regarding the
use of this unit.
•Use only approved accessories. Do not modify system components. Any alterations
or modifications to your incubator may be dangerous and will void your warranty.
•Always plug the unit’s power cord into a grounded electrical outlet that conforms to
national and local electrical codes. If the unit is not grounded, parts such as knobs
and controls may conduct electricity and cause serious injury.
•Do not connect the unit to a power source of any other voltage or frequency beyond
the range stated on the power rating on the dataplate inside the door of the unit.
•Do not modify the power cord provided with the unit. If the plug does not fit an outlet,
have a proper outlet installed by a qualified electrician.
•Avoid damaging the power cord. Do not bend it excessively, step on it, place heavy
objects on it. A damaged cord can easily become a shock or fire hazard. Never use
a power cord after it has become damaged.
•Do not position the equipment in such a manner as to make it difficult to disconnect
power cord or coupler.
•Do not attempt to move the unit while in operation or before the unit has been
allowed to cool.
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RECEIVING AND INSPECTION
IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY.
Your satisfaction and safety require a complete understanding of this unit, including its
proper function and operational characteristics. Be sure operators are given adequate
training before attempting to put the unit in service. NOTE: This equipment must be used
only for its intended application; any alterations or modifications will void your warranty.
1.1 Inspection: The carrier, when accepting shipment, also accepts responsibility for
safe delivery and is liable for loss or damage claims. On delivery, inspect for visible
exterior damage, note and describe on the freight bill any damage found and enter
your claim on the form supplied by the carrier.
1.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If
any, the carrier will arrange for official inspection to substantiate your claim. Save
the shipping crate until you are sure the unit has been delivered in good condition.
1.3 Return Shipment: If for any reason you must return the unit, contact your
customer service representative for authorization and supply nameplate data.
Please see the manual cover for information on where to contact customer service.
1.4 Accessories: Make sure all of the equipment indicated on the packing slip is
included with the unit. Carefully check all packaging before discarding. An
accessory package is included with each unit. The 2310 comes equipped with 3
shelves, 4 shelf standards, 4 leveling feet, 1 humidity pan, and supply hose kit. The
2300 and 2400 comes equipped with 3 shelves, 2 shelf stands and 1 humidity pan
per chamber, 4 leveling feet and 1 supply hose kit. All units are supplied with a
power cord.
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GRAPHIC SYMBOLS
Your incubator has been provided with a display of graphic symbols, which are designed to
help in identifying the use and function of the available user adjustable components.
2.1 Indicates that you should consult your manual for further description
or discussion of a control user item.
2.2 Indicates "Temperature".
2.3 Indicates "Overtemperature Safety".
2.4 °CIndicates "Degrees Centigrade".
2.5 CO2Indicates "Carbon Dioxide".
2.6 Indicates "Gas" (CO2 for this unit).
2.7 Indicates "AC Power ON".
2.8 Indicates "ON/I" and "OFF/O".
2.9 Indicates "Potential Shock Hazard" behind this protective partition.
2.10 Indicates "Protective Earth Ground".
2.11 Indicates "Water Jacket Low".
2.12 Indicates “Unit should be recycled” (Not disposed of in land-fill)
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INSTALLATION
Local city, county, or other ordinances may govern the use of this equipment. If you have
any questions about local requirements, please contact the appropriate local agency.
Installation may be performed by the end user.
Under normal circumstances this unit is intended for use indoors, at room temperatures
between 5°and 40°C , at no greater than 80% Relative Humidity (at 25°C) and with a
supply voltage that does not vary by more than 10%. Customer services should be
contacted for operating conditions outside of these limits.
CAUTION: Make sure that the incubator is located in its intended position and level
before filling the water jacket.
3.1 Power Source: The unit power requirements are listed on the data plate. PLUG
THE UNIT INTO A PROPERLY GROUNDED AND RATED RECEPTACLE OF
THE CORRECT STYLE. A separate circuit is recommended for this unit to prevent
loss of product due to overloading or circuit failures caused by other equipment.
The power supply must be properly grounded (earthed) and correctly sized to match
the unit nameplate rating. The supply voltage must match the nameplate
voltage within +/- 10%. The US market units are designed for 110 volts @ 60 Hz,
5 Amps. The EU market units are designed for 220 volts @ 50 Hz, 3.5 Amps. If
supplied with a detachable cord set, plug the female end into the inlet on the unit
and the male plug into the supply. Assure that units requiring fuses have them
installed. This fuse may be at the inlet or a part of the cord set male plug. The unit
must be located to easily allow for it to be unplugged or disconnected.
3.2 Location: When selecting a site for the unit, consider conditions which may affect
performance, such as heat from steam radiators, ovens, autoclaves, etc. Avoid
direct sun, fast-moving air currents, heating/cooling ducts, and high-traffic areas. To
ensure air circulation around the unit, allow a minimum of 10cm between the
incubator and walls or partitions which might obstruct free airflow.
3.3 Lifting/Handling: These units are heavy and care should be taken to use
appropriate lifting devices that are sufficiently rated for these loads. Units should
only be lifted from their bottom surfaces. Doors, handles and knobs are not
adequate for lifting or transport. All moving parts, such as shelves and trays should
be removed and doors need to be positively locked in the closed position during
transfer to prevent shifting and damage.
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3.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must
be installed in the four holes in the bottom corners of the unit. With the feet installed
and the unit standing upright, each foot can be raised by turning it in a
counterclockwise direction. Adjust the foot at each corner until the unit stands level
and solid without rocking. If the unit must be moved, drain all the water from the
jacket and turn the leveling feet all the way clockwise to prevent damage.
3.5 Cleaning: The unit chamber should be cleaned and disinfected prior to use.
Your operating conditions and appropriate protocol will determine the correct
procedure for decontamination. A typical decontamination procedure that is
adequate for many situations has been described below. As well, certain steps
are listed that will help reduce the likelihood of contamination and the necessity
of decontamination. Whatever process is appropriate, it needs to be done on a
regularly scheduled basis. Depending on usage and protocol, this may be
monthly, quarterly or otherwise. Regardless of the decontamination procedure
used, certain precautions will need to be taken:
A. Always disconnect the unit from the electrical service when cleaning.
Assure all volatile or flammable cleaners are evaporated and dry before
reconnecting the unit to the power supply.
B. Special care should be taken when cleaning around sensing heads to
prevent damage.
C. If you use chlorine-based bleaches or abrasive cleaners this will modify
the stainless steel interior finish. DO NOT USE hard tools such as metal
wire brushes or steel wool. Use non-abrasive cleaners and soft tools such
as plastic brushes.
D. In the event hazardous material is spilled onto or into the equipment,
appropriate decontamination must be carried out. If there is any doubt
about the compatibility of decontamination or cleaning agents with parts of
the equipment or with material contained, please contact the manufacturer
or his agent. No decontamination or cleaning agents should be used which
could cause a hazard as a result of a reaction with parts of the equipment
or with the material contained in it.
TYPICAL DECONTAMINATION PROCEDURE
1. Remove the humidity pan every week and autoclave, or wash with soap
and water then disinfect with 70% alcohol solution. Replace water in the
incubator with fresh, DISTILLED water.
2. Flush the sample port tubing with 70% alcohol solution. Replace any lines
that have a green color.
3. Remove the door gaskets, clean and disinfect. Clean and disinfect all
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mounting grooves for the door gaskets.
4. Remove all shelves, shelf supports, shelf standards and shields.
Autoclave, or wash and disinfect as described in item 1.
5. Wash and disinfect all interior surfaces.
6. Give special attention to cleaning and disinfecting all access ports, air
bleeds, shaft holes, electrical pass-through and any other passages into
the chamber.
7. Replace all air and CO2filters every six months or when noticeably dirty
on the upstream side. CO2 filters are located in the shadow box just
behind the GAS IN fitting and in line with the CO2 tubing kit. Units with
flow meters require operator- supplied air pumps. These pumps should
be checked for proper air filtering.
OPERATION FOR MINIMIZING CONTAMINATION
1. Keep the outside of the incubator, including the air in the laboratory, as
clean as possible. This is particularly important for units placed directly on
the floor. Do not place incubators near doors, air vents or other areas of
high air movement or traffic.
2. The floor around the unit needs to be clean. Units that are placed on the
floor should be mounted higher – typically on a caster platform – for ease
of moving the unit during cleaning and access to the back of the unit.
3. Minimize the number of times access is made to the chamber during
normal operation.
4. Do not depend on the use of antibiotics to maintain uncontaminated
conditions, as this is an inadequate technique for sterilization. Preferably
use aseptic techniques as described above for maintaining sterile
conditions in the incubator.
3.6 Shelves and Interior Parts: Shelving, clips and a humidity pan are supplied with
each unit. See Figure 1 for proper placement of these accessories. Also see
similar shelf assembly instructions sent with the accessory kit.
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Figure 1
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CONTROL PANEL OVERVIEW (See Figure 2)
Units with a detachable cord have a fused inlet located at the top rear of the control panel.
This inlet has a recessed male plug, fuse, and an EMI filtering system designed to filter out
electrical interference. This inlet also prevents any internally generated interference from
feeding out to the power grid. All controls are located on the front panel, except the Over
Temperature Safety which is located at the back.
4.1 Power Switch: The I/O (ON/OFF) switch controls all of the power for the incubator
and must be in the I/On position before any systems are operational. Both
Temperature and CO2displays will illuminate when the power switch is in the ON
position.
4.2 Water Low Light: This pilot lamp is on whenever the internal water jacket float
switch has been tripped to the closed position. When the water drops low enough
the float switch closes the circuit turning on the indicator light.
4.3 Safety Activated light: This pilot lamp is on whenever the Over Temperature
Safety thermostat has been activated and taken control of the element. During
normal operating conditions this indicator light should never be on.
4.4 Temperature Control: This controller is marked C°and indicates the actual
temperature within the chamber to .1°C. The UP/DOWN buttons are used for
imputing the set point, calibrating the display, and muting or unmuting the audible
alarm. The HIGH and LOW alarm indicators will light whenever there is an alarm
condition associated with the temperature within the chamber. The MUTE indicator
will light whenever the audible alarm has been deactivated.
4.5 Heating Light: This pilot lamp is on whenever the Temperature Controller has
activated the heating elements to reach and maintain set point.
4.6 C02 Control On Water Jacket Incubators: The control sets the rate and the
limit of injection of CO2 into the incubator based on input value and some
variables. The input value is set by the operator. This is set at 5% CO2 in
production. The variables are the amount of humidity in the chamber, and the
amount of time the control has been trying to inject CO2.
At start-up, the incubator must be warmed up to operating temperature. This is
set in production 37°C. During warm up, the water tray for humidity should be
filled and placed inside. This allows the humidity to reach 95% before CO2 is
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injected. The TC type CO2 sensor required 95% humidity to correctly measure
the CO2 levels.
The CO2 should be turned OFF during warm up. Failure to do so will cause the
control overshoot the set value input by the operator, the injection value can be
reset by going into cal mode on the main and door temperatures and back out of
cal mode. The unit is then turned off for ten seconds and back on. This
rebooting of the incubator control resets the injection rate and limit values to that
which was input by the operator. The unit would be then be reset to the selected
temperature on main and the door and the CO2 set to the desired level.
4.7 Injection Light: This pilot lamp is on whenever the CO2controller is injecting the
CO2into the chamber.
4.8 Over Temperature Safety Control (OTP): Located at the back of the control panel
adjacent to the power inlet. This is a hydraulic thermostat that is wired between the
Main Temperature Control and the heating element and functions as an override
control. If at any time the Temperature Control fails in the ON position and the
temperature in the chamber rises above its set point, the OTP is activated and
maintains temperature at its own set point. Note that the HEATING indicator will
continue to function under the control of the OTP. It is not recommended that the
unit be allowed to operate for an extended period of time using only OTP as
temperature uniformity will suffer.
4.9 CO2Sample Port: This is located in the upper front corner of the right side of the
incubator. A sample can be drawn to measure the CO2content in the chamber at
this port.
Figure 2
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OPERATION
READ THIS SECTION IN ITS ENTIRETY BEFORE ATTEMPTING OPERATION
IMPORTANT: In order to assure the proper operation of this incubator, it is
essential that you regularly verify the accuracy of the temperature and CO2controls.
Temperature can be confirmed by use of a thermometer inside of the chamber.
CO2can be confirmed with the use of a FYRITE Gas Analyzer or other equipment
such as a gas chromatograph. These must be checked at least on a weekly basis.
Any questions should be directed to a technical service representative. The
temperature, and CO2were calibrated at the factory prior to shipment.
5.1 Power up the unit by attaching the cord set (supplied with the accessories) to the
unit inlet and the building power supply. Attach the female end of the cordset into
the fused and recessed male plug on the rear of the unit and the male plug end of
the cord set into the building supply receptacle. Turn the power switch to the I/ON
position.
5.2 Filling and Draining the Jacket: The front panel of the incubator has a WATER
LOW warning light. Fill the water jacket with WATER by means of the fill port
located at the top right rear of the unit marked FILL WJ HERE. Stop filling when the
WATER LOW warning light goes off. When full, the 2310 holds approximately 9.5
U.S. gallons (35.96 Liters) and 19.0 U.S. gallons (72 Liters) for the 2300/2400
chambers. To drain the unit, hook a suitable hose to the siphon tube located to the
left of the fill port and marked SIPHON HERE. Start a siphon and allow the water to
empty into a suitable service drain or catchment area.
WATER RECOMMENDATIONS FOR USE IN WATER JACKET INCUBATORS
The addition of a dis-similar metal anode in Water Jacket incubators will allow the use of
good-quality tap water to fill the Jacket. The anode is located in a threaded port near
the fill port on the rear of the unit. (diagram). The anode will dissolve as it is in contact
with mineral salts or dissolved gasses in the tap water. The anode should be removed
and checked for erosion every year. When the anode appears to be over 50% eroded,
a new one should be purchased form Sheldon Mfg., Inc. (Replacement Part #0260500)
Tap water with a hardness of more than 30 parts per million (PPM) or 1.5 grains per
gallon (GPG) will require assessment of the anode on a yearly basis. Dissolved gasses
should be no more than 120 PPM or 7 GPG. The Ph of the water should test to
between 6.0 and 8.5. DO NOT USE de-ionized water, it will cause corrosion.
When in doubt, it is advisable to use DISTILLED WATER ONLY.
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5.3 Setting the Controls: Refer to the Control Panel Overview Section 4.6 of this
manual.
5.4 Calibrating the Controls:
A. Push and hold both the UP and DOWN buttons until the decimal points in the
display begin to blink.
B. While the decimal points are blinking the UP and DOWN buttons can be
used to adjust the display to match the actual condition in the incubator
chamber.
C. If no buttons are pressed within five (5) seconds the blinking will stop and the
display will revert to showing the process or actual parameter within the
incubator chamber.
5.5 Humidification: Humidification of the unit is achieved by evaporation of water from
the chamber humidity pan placed in the bottom of the incubator. By filling this
stainless steel reservoir pan with DISTILLED WATER and allowing this water supply
to heat and evaporate, near saturation humidity is obtained. Do not use plastic,
glass or other metals. Only 300 series stainless metals are acceptable for this
reservoir pan. Do not use corrosive chemicals, including copper sulphate or
chlorine, in the pan or chamber as damage may occur. Use Distilled Water Only.
Do NOT use Deionized Water!
Use of disinfecting chemicals in the chamber can change the surface tension of the
reservoir water thus preventing evaporation and proper humidification of the
chamber. Water in the pan should be changed and the pan cleaned at least once a
week to help control contamination and maintain proper surface tension.
5.6 Temperature Monitoring: To insure that the incubator is operating at the desired
temperature, an accurate temperature indicator such as a certified reference
thermometer should be placed in the incubator chamber. Try to place the
thermometer in the center of the chamber and raised off the shelf (taping the
thermometer down to a petri dish is a method that raises it off the shelf and prevents
it from rolling and hiding the scale from view). For ease of reading the thermometer,
it would be better if it was an electronic with a remote display so they can be read
without opening the doors.
5.7 Initial Control Settings: Set the controls to the set points you want your incubator
to operate at.
A. Set the over temperature safety to its maximum position clockwise. Allow
the incubator to warm up and stabilize for 24 hours after making the initial
control settings.
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B. All units are shipped set for 37°C and 0.0% CO2. If other conditions are
desired follow the instructions in section 5.3 for resetting controls.
5.8 Temperature Calibration: It is important that the calibration be performed in the
following order: Main temperature, then the over temperature safety.
A. During main temperature calibration it is important that the inner glass door
not be opened for any reason, and that the outer heated door be opened as
little as possible.
B. After the incubator has been running for 24 hours and the temperature
display is stable at the set point, read the temperature in the incubator
chamber from the reference thermometer and, if necessary, recalibrate the
display using the instructions in section 5.4.
C. After the main temperature display has restabilized and maintained set point
for several hours, check the actual temperature again. If it does not match
the display, repeat the calibration process again.
D. After the Main temperature is set and adjusted, the over temperature safety
control needs to be set. Do this by turning the Safety counterclockwise until
the SAFETY ACTIVATED indicator light comes on. Next slowly turn the
control knob clockwise until the indicator light turns off. Then turn clockwise
two of the smallest divisions on its scale past the point where the indicator
went out. This will set the Over Temperature Safety at approximately 1°C
above the Main Temperature Controller.
5.9 CO2Supply System and Control System: The CO2system is rated for
pressures between 15 and 20 PSI, which should never be exceeded at any time.
Prior to any attempt to hook up or run the CO2Control System, the water jacket
must be filled and the temperatures adjusted as noted in Section 5.7 and 5.8.
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Figure 3
9850677
A. The CO2inlet fitting is located on the back of the incubator near the top (see
figure 3). It is marked "CO2TO CHAMBER". The supply hose with in-line
CO2filter (supplied with your accessories) connects from this fitting to the
CO2tank and regulator. The initial supply pressure should be 20 PSI.
B. It is highly recommended that a good quality DUAL STAGE pressure
regulator be used on the CO2tank. The dual stage regulator will have two
pressure gauges with a 0-60PSI LOW-PRESSURE range. The high
pressure gauge will indicate the pressure within the tank. The low pressure
gauge will indicate the pressure on the supply hose to the incubator. Do not
use single stage regulators. A two gauge regulator is not necessarily a dual
stage regulator. Insure that you are using a dual stage regulator.
C. It is normal for the high pressure gauge on your regulator to start out reading
800 to 1000 PSI with a full tank. This reading will drop to 500 to 800 PSI
quickly and will stay there for most of the duration of the tank. At the end of
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use, the pressure will drop quickly to zero, indicating that the tank is
completely empty. Pure CO2is in a liquid state in the new tank and a
constant vapor pressure is generated in the tank above the liquid level, and
the CO2is drawn from the top as a gas. This same vapor pressure is
maintained as long as any liquid is left in the tank. When the last of the liquid
has evaporated into gas then the pressure will drop rapidly as the gas is
drawn off.
D. Only medical grade CO2should be used in your incubator. The use of a
lesser grade may damage your incubator and void your warranty.
E. The microprocessor CO2control system interprets the information from the
CO2sensor, displays the CO2concentration directly on the digital display,
reads the CO2set point, and controls the percentage of CO2in the incubator
chamber.
F. There are two types of CO2sensors available, Thermal Conductivity (TC)
and Infrared (IR). The 2310 and 2300 units operate with TC sensors, and
the 2400 units operate with IR sensors.
G. TC sensors operate under the principle that CO2is 1.5 times denser than air
and if you measure the changes in density they can be interpreted as
changes in CO2content. This method of measurement is accurate, reliable
and stable as long as all factors that influence air density are maintained
unchanged. Factors that effect density are humidity and temperature. When
the door of the incubator is opened, the humidity is reduced, the temperature
is lowered and the thermal conductivity control system loses stability until the
conditions in the incubator have recovered to original conditions.
H. IR sensors operate under the principle that a certain frequency of infrared
light is absorbed by CO2. The more CO2is present the more light is
absorbed. Advantages to this system are that it is only sensitive to CO2, so
its accuracy is consistent no matter what the conditions are in the incubator.
I. Both types of CO2sensors are factory calibrated and under normal
circumstances need no calibration in the fields; however, it is recommended
that the accuracy of your CO2control system be monitored by measuring the
actual CO2concentration on a weekly basis with a FYRITE or other
measuring device. This should be done when the chamber has not been
disturbed for an extended period of time, i.e. after the weekend or first thing
on Monday morning.
5.10 Setting the CO2Control: Attach the supply hose to the incubator and turn on the
CO2supply. Set the CO2control to the desired set point using the procedure
described in section 5.3.
5.11 Adjusting CO2Display: After the incubator has had several hours to stabilize at
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CO2set point, measure the actual CO2% with a Fyrite. If there is any difference
between the Fyrite and the display, use the procedure described in section 5.4 for
making the display match the Fyrite. See Section 6.0 for Fyrite use.
NOTE: When using the Fyrite, insure that gas is not being injected while the reading
is being taken. Change the CO2set point to 0.0 prior to taking the sample and then
change the set point back to the desired value after the use of the Fyrite.
5.12 Alarm Conditions: The temperature control and the CO2control are both equipped
with visual and audible alarms.
A. Temperature Alarms: The temperature control has alarm indicators for high
and low conditions that are activated whenever the actual temperature is 1C°
above or below the set point.
B. CO2Alarms: The CO2controller has alarm indicators for high and low alarm
conditions that are activated whenever the actual CO2% is 1% above or
below the set point.
C. Audible Alarms: Both controls are equipped with audible alarms that are
activated when either of the HIGH or LOW indicators are activated. The
audible alarms can be muted for a single alarm occurrence by pressing and
holding down either the UP or DOWN button for several seconds until the
MUTE light comes on. There is a built in delay of 15 minutes on the
occurrence of a LOW alarm. This prevents the audible alarm from activating
every time the door is opened and the temperature and CO2drops.
5.13 Description of nGS (NO Gas Warning) Function: (If equipped)
The CO2 Low alarm activates 15 minutes after the CO2 level drops below 4%, if
the cause is not corrected in 5 minutes the CO2 display will flash nGS, indicating
a no gas warning. nGS will continue to flash until the CO2 level has risen above
4%. The auditable alarm can be muted but the visual low alarm indicator will
continue to be lit and the display will continue to flash nGS until cleared. No
action is required by the user other than to correct the cause of the CO2 to drop.
Note: It may take several hours after loss of gas before the CO2 low alarm
comes on. This depends on the unit’s rate of decay. Low CO2 alarm does not
activate until the CO2 is below 4%.
Restore gas supply. Check CO2 tank and gas supply lines to the unit.
As soon as the gas supply is restored and the CO2 level rises above 4.0%, the
display will stop blinking nGS and will again show the sensor reading. No action
is required by the user other than correcting the cause of the drop in gas supply.
The auditable alarm can be muted but the visual low alarm indicator will continue
to be lit and the display will continue to flash nGS until cleared.
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FYRITE CO2 CHECKING
A Bacharach FYRITE CO2Gas Analyzer is recommended to measure CO2concentrations
in the incubator chamber. This test instrument is not supplied with the incubator but is
readily available from your dealer. Follow the instructions provided with the Fyrite
instrument carefully to insure correct and accurate readings.
1. Press button on top of Fyrite canister to release CO2concentration. Tip canister
to the side to ensure all fluid is released from the top of canister.
2. Loosen screw on slide scale and align top of fluid with zero on the scale. Tighten
screw.
3. Connect hose and aspirator bulb to unit being tested. The sample port for
connection is located in the upper front corner of the right side of the incubator.
4. Place the hose sampling cap directly over the plunger valve on top of canister
and depress firmly.
5. With button depressed, squeeze bulb 27 times. On the last squeeze and with
bulb still deflated, release hose from button.
6. Turn Fyrite canister upside down 3 times, each time allowing all fluid to flow to
the opposite end of the canister.
7. Tip canister to the 45 degree position to ensure all fluid has been released from
top of canister.
8. Read CO2concentration in %.
NOTE: Your Fyrite indicator will come with a complete set of detailed instructions
which should be followed carefully. The fluid used inside this Fyrite instrument is
poisonous and corrosive and must not be taken internally. In event of a spill or
accidental body contact with the Fyrite fluid, follow instructions on the refill bottle
carefully.
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