VWR International INCU-Line 68R User manual

I
I
IN
N
NS
S
ST
T
TR
R
RU
U
UC
C
CT
T
TI
I
IO
O
ON
N
N M
M
MA
A
AN
N
NU
U
UA
A
AL
L
L
VWR Cooled Incubators
INCU-Line 68R: 390-0728
INCU-Line 150R: 390-0729
INCU-Line 250R: 390-0731
Before using the equipment, please read carefully
this instruction manual!
Version 3.6

Instruction Manual INCU-Line Cooled
Page 2/ 32
Legal Adress of Manufacturer:
Country of Origin: Poland

Instruction Manual INCU-Line Cooled
Page 3/ 32

Instruction Manual INCU-Line Cooled
Page 4/ 32

Instruction Manual INCU-Line Cooled
Page 5/ 32
Contents
1IMPORTANT INFORMATION FOR THE USER .............................................................7
2PACKAGE CONTENTS .................................................................................................7
3BEFORE THE FIRST USE.............................................................................................8
3.1 Installation of shelf...................................................................................................9
3.2 Remarks on the type of samples..............................................................................9
3.3 Remarks on the placement of samples..................................................................10
4DEVICE DESCRIPTION...............................................................................................11
4.1 Control panel.........................................................................................................12
4.2 Programming possibilities......................................................................................13
5OPERATING THE DEVICE ..........................................................................................14
5.1 Start-up..................................................................................................................14
5.2 Temperature control...............................................................................................14
6HOW TO OPERATE THE CONTROLLER ...................................................................15
6.1 Simple mode..........................................................................................................15
6.1.1 To stop the unit in the simple mode....................................................................15
6.1.2 To start the unit in the simple mode....................................................................15
6.2 Switching between simple and complex modes.....................................................16
6.3 Complex mode.......................................................................................................16
6.3.1 Editing program parameters...............................................................................16
6.3.2 To start a saved program ...................................................................................18
6.3.3 To stop a program..............................................................................................18
7SETTINGS OF DEVICE................................................................................................18
8TEST RESULTS MEMORY..........................................................................................20
9BASIC CONTROL SOFTWARE...................................................................................21
10 STATISTICS................................................................................................................. 22
10.1 Preview of statistics. ..............................................................................................22
11 ALARMS ......................................................................................................................23
11.1 To set an alarm......................................................................................................24
11.1.1Clock alarm........................................................................................................24
11.1.2Power alarm.......................................................................................................24
12 TEMPERATURE PROTECTION ..................................................................................24
13 OPERATION OF THE COOLING SYSTEM..................................................................25
14 CLEANING AND MAINTENANCE OF THE DEVICE ...................................................26

Instruction Manual INCU-Line Cooled
Page 6/ 32
14.1 Housing cleaning...................................................................................................26
14.2 Interior cleaning.....................................................................................................27
15 RESUMING OPERATION AFTER A LONGER PERIOD OF TIME ..............................27
16 TROUBLESHOOTING .................................................................................................28
16.1 REPLACEMANT OF FUSE....................................................................................29
17 PRODUCT IDENTIFICATION LABEL..........................................................................29
18 WARRANTY CONDITIONS..........................................................................................30
19 TECHNICAL DETAILS.................................................................................................31

Instruction Manual INCU-Line Cooled
Page 7/ 32
1 IMPORTANT INFORMATION FOR THE USER
To guarantee your security and the longest efficiency of the unit, please comply with the following rules:
1.
The unit cannot be installed:
•outside,
•in damp places or places which can be easily flooded,
•near flammable or volatile substances,
•near acids or in corrosive environments.
2.
It is forbidden to:
•store inflammable or volatile substances inside the unit,
•touch live parts of the unit
•operate the unit with wet hands,
•put water vessels on the unit,
•climb or put any objects on the unit,
•do not overload the shelves ( the maximum load is described in technical data)
3.
You should:
•place samples in such a way to provide proper air circulation in the chamber
•open the door for the shortest period of time to reduce temperature fluctuations
•defrost the chamber more often if you open the door a lot or store humid samples
•if possible, do not store warm samples
•secure samples from being blown out by the chamber fan e.g powdery samples
•always check that the doors are closed
•use only mains with earth to avoid electric shocks,
•unplug the power cable holding the protective cover and not the cable itself,
•disconnect the unit from the mains before undertaking any repairs or maintenance works,
•protect the power cable and the plug from any damage
•disconnect the power plug before moving the unit,
•disconnect the power plug if the device will not be used for a long period of time,
•disconnect the unit and protect it from reconnecting if it has any visual faults.
2 PACKAGE CONTENTS
INCU-Line devices are delivered with the following equipment:
INCU-Line 68R, 2 wire shelves with set of slides –2 pcs. each, keys to the lock (2pcs.), Manufacturer test
certificate at 37oC, Multilingual instruction manual and Basic Control Software (on CD) for download data
from internal memory
INCU-Line 150R, 2 wire shelves with set of slides –2 pcs. each, 1 bottom wire shelf with set of slides (2
pcs.), keys to the lock (2pcs.), manufacturer test certificate at 37oC, Multilingual instruction manual and
Basic Control Software (on CD) for download data from internal memory
INCU-Line 250R, 3 wire shelves with set of slides –2 pcs. each, 1 bottom wire shelf with set of slides (2
pcs.), keys to the lock (2pcs.), manufacturer test certificate at 37oC, Multilingual instruction manual and
Basic Control Software (on CD) for download data from internal memory

Instruction Manual INCU-Line Cooled
Page 8/ 32
3 BEFORE THE FIRST USE
On the surface of unit components made of stainless steel, slight discoloration may occur. It is a result of
the technologies used in the production of metal sheet in accordance with the requirements of PN-EN
10088-2 standard and it is not a defect of the unit.
While carrying the unit, please do not tilt it to one side more than 45° from the upright position,
as there is a high probability of the damaging the compressor. If it is necessary to tilt it to one
side more than 45°, then after placing it, please wait at least 3 hours before connecting the unit
to the mains.
The place of installation of the unit should meet the following conditions:
•Ambient temperature +10°C...+28°C, for INCU-Line 68R (with glass door) +10°C...+25°C,
•Low relative humidity of the ambient air to 60%
•The unit has not been designed to work in highly dusty environments
•The place of installation should be appropriately ventilated according to its size
•The unit should be put on a hard and stable substrate
•The unit should be placed at least 100mm away from the wall
•The height of the room must be at least 300mm greater than the height of the unit
•This unit may not be exposed to direct sunlight
•The unit should be kept away from any heat sources
•The unit is not designed to be built-in
•The place of installation of the unit should contain a mains socket.
If you don’t comply with the above recommendations, the unit may get broken or it may worsen the tech-
nical parameters.
The electric installation should meet the following conditions:
The electric installation should be secured by a 16 A antisurge fuse.
The device is powered by AC 230V/50Hz. Please connect it to a socket with ground in order
to avoid electric shocks in case of the unit’s failure.

Instruction Manual INCU-Line Cooled
Page 9/ 32
3.1 Installation of shelf
Perform the following steps to adjust shelf height:
1) Install the shelf holder at the selected height by inserting it into proper slots on the wall.
2) Insert the shelf into the holder on one side.
3) Slide the second shelf holder onto the other side of the shelf.
4) Insert the shelf holder into proper slots on the other wall.
To remove a shelf, perform the above steps in reverse order. To remove the shelf holder from the slots, lift
it up and slide it towards the rear of the chamber.
3.2 Remarks on the type of samples
Water may gather on the bottom of the chamber. It is a result of condensation of the water vapor located in
air if the set temperature is considerably lower than the ambient temperature.
The amount of water depends on the following factors:
- Differences between ambient and set temperatures
- Number and frequency of door openings
- Temperature of samples
2
1
4
3

Instruction Manual INCU-Line Cooled
Page 10 / 32
Do not use any cardboard boxes, sponges and other hygroscopic materials for storing the samples since
they may increase the relative humidity in the chamber.
3.3 Remarks on the placement of samples
To provide proper air circulation and stable conditions in which the samples are stored in the chamber, it is
necessary to keep the following rules:
- the max height of the samples should not exceed 1/3 of the space below the shelves
- the samples should be placed in such a way that so that the horizontal surface between the
containers does not exceed 1/3 of the width and height of the empty shelf
- the space between the samples and between the samples and the wall should be more or less
equal
The picture below is an example of the placement of samples in the chamber.
Hmax
H
Hmax
a
uv w
xy z
b
u+v+w > 1/3 a
x+y+z > 1/3 b
Hmax = 2/3H
u v w
x y z
H
≅
≅ ≅
≅
Front view
Top view
Shelf
Following the above rules will provide best optimal parameters of temperature stability and uniformity.
If water gathers, use a dry cloth to wipe the bottom of the chamber.

Instruction Manual INCU-Line Cooled
Page 11 / 32
4 DEVICE DESCRIPTION
(1) RS-232C and USB socket
(2) Electronic controller with LCD display
(3) Chamber fan
(4) Access port (30mm)
(5) Shelf
(6) Key lock
(7) Main switch
(8) Internal electrical socket
(9) Internal lighting LED
(10) Temperature sensor
(11) Height adjustable feets
(12) Mains socket (for power cable)
(13) Condenser
(14) Compressor
13
14
12
7
10
1
2
3
4
5
6
8
9
11

Instruction Manual INCU-Line Cooled
Page 12 / 32
4.1 Control panel
The control panel is used to check the current temperature inside the chamber as well as to program and
set up the parameters of the device.
(1)
(1) Quick turn on/off button
(2) Alarm signalization (red LED)
(3) LCD graphical display
(4) Escape/exit button
(5) Down button
(6) Up button
(7) Enter/accept button
The value may be changed by the following buttons: / up/down (selection keys). Confirm using the
enter button. The program continues to set the next parameter. If the parameter value is incorrect, us-
ing the selection keys / up/down select the parameter again and set the correct value. To exit the
current window, please press down the ESC button.
Internal lighting LED
Internal lighting is switched on automatically when the outside door is open. For INCU-Line 68R the internal
light can be switched on even when the glass door is closed. Press down the enter button and hold it
for about 2 sec to switch on the light. Once the light has been switch on, a bulb icon will appear on the dis-
play.
1
2
3
4
5
6
7

Instruction Manual INCU-Line Cooled
Page 13 / 32
4.2 Programming possibilities
There are two temperature program modes of the device: simple and complex. In the simple mode the
stress is on maximum simplification of the programming procedure. The only parameter needed to start the
program is setting up the desired temperature.
The complex mode offers more possibilities in terms of programming the temperature, it is also possible to
set up a six-segment time-temperature program.
Programming mode is defined in the menu settings (parameter Prog.mode )
The possibilities of the program controlling the operation of the device depend on the type of the device.
The most advanced software allows storing up to 3 six-segment pro-
grams.
Simple mode
Temperature
Time
Tp
To
Tp –set temperature
To –ambient temperature
Operating the device in this mode is extremely simple, with temperature being the only parameter needed
to set up.
Complex mode
The complex mode is used when there is a need to conduct research requiring varied temperature with
specific duration. In this mode you can also store programs consisting of a maximum of six segments. For
each segment you can set up temperature and its duration. For units with photoperiodic system you may
also switch on/off the light inside the chamber.
Device series
IL
Number of programs
3
Number of segments in
each program
6

Instruction Manual INCU-Line Cooled
Page 14 / 32
5 OPERATING THE DEVICE
5.1 Start-up
The main switch is located on the left side of the front panel.
To enter the parameters, please use the control panel which is located in the upper front part of the unit.
After the unit has been switched on, there is a self test of the following parts performed: EEPROM memory,
Data Flash memory, real time clock RTC, and a temperature sensor. After a successful auto-test, you will
see the main screen on the display:
- unit working in quick mode (see point 7.1)
- unit working in complex mode (see point 7.3), program 1 is active.
If the device has been switched off during an active program, it will automatically resume once the
device has been switched on again.
- no program is being carried out.
Start-up
To start-up the unit, please perform the following sequence:
after connecting the unit to the mains, please switch it on using the on/off switch
please press down the button which is located below the control panel after a successful start-up
Switching off the unit
please press down the button which is located below the control panel
after a successful shut down, there is no illumination of the display
the user may switch off the unit completely using the on/off switch located in the front panel
5.2 Temperature control
The device has been adjusted and calibrated. The calibration is carried out in accordance with the manu-
facturer's procedures and instructions, using instruments which are inspected regularly.
Temperature is measured by a sensor built inside the chamber and its value shows on the display of the
control panel. The device has been calibrated in such a way that the display shows the temperature in the
middle of the chamber.
Use the main switch to turn off the unit only if you are not going to use it for a longer period of time
(e.g. a couple of days) or for maintenance.

Instruction Manual INCU-Line Cooled
Page 15 / 32
6 HOW TO OPERATE THE CONTROLLER
6.1 Simple mode
After the unit has been turned on, you can see the set temperature on the display. You can change the
value using the UP/DOWN / buttons. Press ENTER to confirm.
Example:
Press UP or DOWN to edit the temperature.
The temperature will flicker. Use UP or DOWN to set a new
temperature. Press ENTER to confirm.
Use UP or DOWN to set the decimal value and confirm with
ENTER .
A new temperature has been set.
You can press ESC at any time to cancel.
6.1.1 To stop the unit in the simple mode
Press ESC twice. You will see STOP on the display. Confirm with ENTER .
6.1.2 To start the unit in the simple mode
In the main window, please press ESC twice. You will be able to set the temperature as described
above. Once set, press ENTER to confirm. The unit will start achieving the set temperature.

Instruction Manual INCU-Line Cooled
Page 16 / 32
6.2 Switching between simple and complex modes
The complex mode is used when there is a need to conduct research requiring varied temperature with
specific duration. In this mode you can also store programs consisting of a maximum of six segments. For
each segment you can set up temperature and its duration.
To switch from simple to complex mode, perform the following steps:
In the main window press ESC to enter the MENU.
Enter SETUP using ENTER . Press down and choose
PROG.MODE.
Press ENTER . The setting will flicker.
Press and choose >complex<.
Confirm with ENTER .
To switch from complex to simple mode, perform the above steps analogically.
6.3 Complex mode
6.3.1 Editing program parameters
It is impossible to make changes to a currently running program –it needs to be terminated
first.
You can press ESC at any time to cancel the adjustment.

Instruction Manual INCU-Line Cooled
Page 17 / 32
In the main window press ESC twice.
Enter PROGRAMMING using ENTER .
Choose the program you want to edit using and .
Press ENTER , then use and to set the number of
segments. Confirm with ENTER .
Then you can choose the set temperature for segment 1. Use
and to set a new value, then confirm with ENTER . Set
the decimal value in the same way.
Now you can set the segment time in dd:hh:mm format.
If the program has more than one segment, you can set its pa-
rameters here.
Here you can set start delay in hh:mm format if required.
Here you can set if the program should run in cycles:
- it can be OFF, the program will stop after finishing
- infinite cycles –the program will continue to run till it is stopped
by the user

Instruction Manual INCU-Line Cooled
Page 18 / 32
- you can define the number of cycles between 2 and 99
After all parameters have been set, you can start the program.
Use ENTER to confirm.
6.3.2 To start a saved program
In the main window, press .
Using and choose the program you would like to start and
press .
6.3.3 To stop a program
In the main window press twice.
Confirm with .
7 SETTINGS OF DEVICE
To set up working parameters of the device go to the ‘SETUP’ menu. Using / the UP/DOWN and
then ACCEPT buttons you can change the relevant options.
In the main window press ESC twice to enter MENU.

Instruction Manual INCU-Line Cooled
Page 19 / 32
Choose SETUP using .
Press ENTER to enter the menu.
Description of options available in the ‘SETUP’ menu:
TIME ALARM, TEMP ALARM.,
POWER ALARM,
More information in 10.1
[hh:mm], first you set up the hour, then the minutes. You change the
value using / the up/down buttons and confirm with the ac-
cept button. The program automatically moves to setting the next val-
ue.
After selecting a sub-menu the user is able to choose a protocol for
each socket separately –Please press the button. Available proto-
cols:
- none (interface off)
- text (protocol for printers with serial port, terminal),
- service (service and text protocol).
- dedicated software
After selecting a protocol with / buttons, the user is able to de-
termine its settings.
- Print interval: [mm:ss] determines the interval after which data is
sent to the serial port (available in service and text protocols),
- address [1-255] –determines the address of the instrument con-
nected to the network
selecting the type of programming the device, there are two options
available ‘simple’– simple mode and ‘complex ‘ – complex mode of
programming the device. See point 5.4.
There is a possibility to choose controller backlight mode. “Fixed”
mode means constant backlight. “Temporary” mode means that the
backlight will go off in approximately one minute from the last bottom
touch.

Instruction Manual INCU-Line Cooled
Page 20 / 32
8 TEST RESULTS MEMORY
In MENU choose LOGGER
To choose from DATA or EVENTS.
In the menu you can set the interval time of saving temperature in the
chamber. Note <00:15> means, that every 15 minutes data of tempera-
ture will be registered in internal memory (max 2048 results). If the
memory will be fulfilled, the data will be over written.
Erase the data.
Reading the data from logger is possible by using a PC application –
Basic Control
Event window opens the internal event logger.
Command ‘view will display events like: start, stop or end of program
as well as loss and return of power, alarm of over temperature, door
openings.
Sample of displayed events and occur time of the event. After pressing
and pushing the date of the event will be displayed.
Erasing the event from the memory (max. numbers of saved events is
110), If the memory will be fulfilled, the data will be over written.
Reading the data from logger is possible by using a PC application –
Basic Control.