VWR International sympHony User manual

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Sampling Equipment
Decontamination
Number: SOP-E-044
Page 1 of 5
Revision: 1
Date: --/--/--
APPROVAL PAGE
SAMPLING EQUIPMENT DECONTAMINATION
APPROVALS
Environmental Coordinator:
Date:
Program Manager:
Date:
QA Manager:
Date:

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Sampling Equipment
Decontamination
Number: SOP-E-044
Page 2 of 5
Revision: 1
Date: --/--/--
SAMPLING EQUIPMENT DECONTAMINATION
1.0 PURPOSE
The purpose of this procedure is to describe the methods for decontamination of non-
dedicated sampling equipment used in association with water quality monitoring and soil,
sediment or vegetation sampling.
2.0 SCOPE
This procedure applies to the decontamination of all sampling equipment that is used
during water, soil, sediment or vegetation sampling associated with activities of the
Cotter Corporation (N.S.L.) (“Cotter”), Canon City Milling Facility. It applies only to
equipment that is not dedicated to an individual sampling site.
3.0 REFERENCES
3.1 Current Colorado Radioactive Materials License.
3.2 Quality Assurance Project Plan (QAPP), current version.
4.0 DEFINITIONS
4.1 Reagent Grade Water: ASTM Type 2 water prepared by distillation or other
equal process, followed by polishing with a mixed bed of ion exchange materials
and a 0.2 micron membrane filter, such that the resulting water has a maximum
electrical conductivity of 1 µS/cm at 25˚C from the lab or a minimum electrical
resistivity of 100 MΩ/cm at 25˚C.
4.2 EDTA: ethylene diamine tetra acetic acid.
4.3 Field Staff: the sampling technician or other qualified individual designated by
the Environmental Coordinator (EC).
5.0 EQUIPMENT AND MATERIALS
5.1 EQUIPMENT
5.1.1 Plastic tubs or buckets.
5.1.2 Scrub brushes, plastic or fiber bristles.
5.1.3 Wash solution bottle(s).
5.2 MATERIALS

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Sampling Equipment
Decontamination
Number: SOP-E-044
Page 3 of 5
Revision: 1
Date: --/--/--
5.2.1 Non-Phosphate, non-ionic cleaning solution containing EDTA.
5.2.2 Disposable wipes or paper towels.
5.2.3 Deionized water.
5.2.4 Tap water.
5.2.5 0.1 N HCL (hydrochloric acid solution).
5.2.6 Plastic bags.
5.2.7 Brush.
5.3 MAINTENANCE/CALIBRATION
5.3.1 None for this procedure
6.0 HEALTH AND SAFETY
6.1 Acids are potentially dangerous chemicals. Read the individual Safety Data Sheet
(SDS) for each chemical before use.
6.2 Store all acids according to manufactures recommendations.
6.3 Wear latex gloves and safety glasses when preparing and handling acids.
7.0 RESPONSIBILITY
7.1 It is the responsibility of the EC to make sure that personnel performing this task
are properly trained.
7.2 It is the responsibility of field staff personnel conducting this procedure to follow
it.
8.0 INSTRUCTIONS
8.1 This procedure should be used after sampling at each site to eliminate the
potential for cross contamination of future samples.
8.2 When choosing a site for decontamination, avoid fugitive dust, oils, gasoline, fuel
oils, organic solvents, sources of radioactive contamination, and any other
possible source of contamination, which may affect equipment or sample
integrity.

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Sampling Equipment
Decontamination
Number: SOP-E-044
Page 4 of 5
Revision: 1
Date: --/--/--
8.2.1 If decontamination is performed at a site other than the location where the
equipment was used, carefully transport equipment to the decontamination
area in a manner that minimizes the spread of contaminants.
8.3 For water quality monitoring, use the following sequence for equipment used with
organic and inorganic target analytes.
8.3.1 Remove any gross contamination such as loose soil with brush and tap
water.
a. Non-dedicated pumps will have solution pumped through it and all
associated sampling lines
b. Filtration equipment will be disassembled and washed with a
bristle brush.
c. Stainless, Teflon or other containers used for sampling will be
washed with a bristle brush.
8.3.2 Wash all sampling equipment with a non-Phosphate, non-ionic cleaning
solution containing EDTA, example, Liquinox8in concentrations
recommended by the manufacture.
8.3.3 Rinse or pump 0.1 N HCL (hydrochloric acid solution) or 0.1 N HNO3
(nitric acid solution) through or on equipment.
8.3.4 Rinse or pump tap water through or on equipment thoroughly.
8.3.5 Rinse or pump Type 2 reagent water on or through equipment.
8.3.6 Measure the conductivity of the Type 2 water rinse.
a. If measured conductivity is <50 uS/cm the decontamination is
deemed adequate, otherwise repeat the cleaning procedure.
8.3.7 Store equipment to prevent contaminating equipment for next use.
8.4 For vegetation, soil or sediment sampling use the following sequence for
equipment decontamination.
8.4.1 Remove any gross contamination such as loose soil, sediment or
vegetation with brush and then rinse with Type 2 reagent water.
8.4.2 Wipe dry with paper towel or let air dry before moving to next sampling
location.
8.4.3 Store equipment to prevent contaminating equipment for next use.

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Sampling Equipment
Decontamination
Number: SOP-E-044
Page 5 of 5
Revision: 1
Date: --/--/--
9.0 RECORDS
9.1 For water quality monitoring record the measured field conductivity value from
the QA rinsate in the field sampling logbook or equivalent.

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Oxidation Reduction
Potential Measurements
Number: E-046
Page 1 of 6
Revision: 1
Date: --/--/--
APPROVAL PAGE
OXIDATION REDUCTION POTENTIAL MEASUREMENTS
APPROVALS
Environmental Coordinator:
Date:
Program Manager:
Date:
QA Manager:
Date:

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Oxidation Reduction
Potential Measurements
Number: E-046
Page 2 of 6
Revision: 1
Date: --/--/--
1.0 PURPOSE
This procedure describes the activities necessary for measuring the Oxidation-reduction
potential (ORP) in unfiltered water samples during water quality monitoring activities at
the Cotter Corporation (N.S.L.) (“Cotter”), Canon City Milling Facility.
2.0 SCOPE
This procedure applies to oxidation-reduction potential (ORP) measurements within a
millivolt range of +/- 1999.9 mV in water samples taken for water quality measurements
by Cotter Corporation (N.S.L.), Canon City Milling Facility.
3.0 REFERENCES
3.1 Quality Assurance Project Plan, current version.
3.2 Colorado Radioactive Materials License, current version.
4.0 DEFINITIONS
4.1 Oxidation-reduction potential (ORP) measurements are used to monitor chemical
reactions, quantify ion activity or determine the oxidizing or reducing properties
of solutions. While ORP measurements are somewhat similar to those of pH, the
potential value must be carefully interpreted to achieve meaningful results. The
field measured ORP values are corrected to correlate to Standard Hydrogen
Electrode (SHE).
5.0 APPENDICES
5.1 ORP reference solution offset correction addition factors.
5.2 VWR SympHony SP80PC Meter Instruction and Operating Manual
6.0 EQUIPMENT AND MATERIALS
6.1 EQUIPMENT
6.1.1 VWR SympHony SP80PC Meter or the equivalent.
6.1.1.1 This meter measures millivolts in the range of +/- 1999.9 mV.
6.1.1.2 If the reading exceeds this range, an error message is displayed.
6.1.2 Refillable Platinum combination ORP electrode, or the equivalent
6.2 MATERIALS

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Oxidation Reduction
Potential Measurements
Number: E-046
Page 3 of 6
Revision: 1
Date: --/--/--
6.2.1 Electrode uses a 4M KCL reference solution saturated with Ag/Ag CI.
6.2.2 Thermo Scientific ORP Standard Solution.
6.2.3 Wash bottle with ASTM Type 2 water.
7.0 HEALTH AND SAFETY
7.1 Refer to the Safety Data Sheet (SDS) for chemical reagent handling precautions.
7.2 ORP standard will stain hands and clothing. In case of contact, flush with affected
area with water.
8.0 RESPONSIBILITY
8.1 Field Sampling technicians are responsible for following this procedure when
measuring the ORP of water samples.
9.0 EQUIPMENT INSPECTION
9.1 Refer to specific pH/mV meter operating manual for troubleshooting and
maintenance.
9.2 Check the meter before taking it into the field.
9.3 Visually inspect the instrument, examining all cables and probes for damage.
9.4 Connect the electrode to the meter.
10.0 EQUIPMENT CALIBRATION AND FREQUENCY
10.1 Check the calibration of the instrument each time it is turned on or at each
sampling location. Recalibrate when needed.
10.2 Insert the electrode into the ORP reference standard solution. Make sure standard
has not expired.
10.3 Record the following information in the field sampling logbook or equivalent:
10.3.1 Ttemperature of the ORP reference solution.
10.3.2 mV reading of reference solution during calibration.
10.3.3 Date and time of calibration in the field sample logbook or equivalent.

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Oxidation Reduction
Potential Measurements
Number: E-046
Page 4 of 6
Revision: 1
Date: --/--/--
10.4 If the mV meter will not calibrate refer to the specific meter/instrument operating
instruction manual for troubleshooting and maintenance information or replace
the meter or probe with a spare meter or mV probe.
11.0 MEASUREMENT INSTRUCTIONS
Direct measurements for ORP are made by simply taking a measurement of a sample,
correcting to units of Standard Hydrogen Electrode (SHE) and reporting a result.
11.1 Direct Measurement Of Samples.
11.1.1 Insert a proper functioning electrode into the water sample to be measured.
11.1.2 Take a reading in mV by following the directions in the pH/mV meter
operator's manual.
11.1.3 When the reading stabilizes, record the sample temperature and mV value
as displayed on the meter.
11.1.4 It is necessary to report the value relative to the results achieved by a
"normal standard hydrogen electrode" as the reference electrode. To do
this you must add the observed mV result to the potential of the reference
electrode being used - in this case a 4 M KCL with Ag/Ag CI electrode,
which has a value of 200 mV at 25oC. This is necessary since the value of
the "normal standard hydrogen electrode” is defined as having a value of
0.0 mV under the same conditions.
11.1.5 Refer to appendix 5.1 for the offset correction factor to use for
measurements at temperatures different than 25oC
Where
Eh = the value of the potential of the sample relative to the “normal
standard hydrogen electrode”
Eo = the potential observed with the ORP electrode (the result from the
meter)
11.1.6 Cf= Correction Addition Factor is the constant offset factor in potential
due to the reference electrode in use (in this example 200 mV for a
temperature of 25oC).
11.1.7 If your meter reported a value of 450 mV at a temperature of 25oC, your
reported result would be Eh = 450 + 200, or 650 mV (SHE). If the value
reported was -599 at a temperature of 25oC your reported result would be
Eh = -599 + 200, or -399 mV (SHE).

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Oxidation Reduction
Potential Measurements
Number: E-046
Page 5 of 6
Revision: 1
Date: --/--/--
11.1.8 After the samples mV measurement has been recorded, remove the
electrode from the sample and rinse completely with de-ionized water.
11.1.9 You can proceed to the next sample or store the electrode.
12.0 RECORDS
12.1 Field sampling logbook, field data sheet, or tablet.

Cotter Corporation (N.S.L.)
Cañon City Milling Facility
Cañon City, Colorado
Procedure
Oxidation Reduction
Potential Measurements
Number: E-046
Page 6 of 6
Revision: 1
Date: --/--/--
Appendix 5.1
ORP Reference Solution Offset Correction Addition Factors
Temp%
(0C)%
ORP%
Standard%
Solution%
*(mV)%
Eh%(SHE)%
(mV)%
Correction%
Addition%
Factor%
%
Temp%
(0C)%
ORP%
Standard%
Solution%
(mV)%
Eh%(SHE)%
(mV)%
Correction%
Addition%
Factor%
%
Temp%
(0C)%
ORP%
Standard%
Solution%
(mV)%
Eh%(SHE)%
(mV)%
Correction%
Addition%
Factor%
0%
218%
438%
220%
%
17%
219%
426%
207%
%
34%
220%
412%
192%
1%
218%
437%
219%
%
18%
219%
425%
206%
%
35%
220%
411%
191%
2%
218%
437%
219%
%
19%
219%
424%
205%
%
36%
220%
410%
190%
3%
218%
436%
218%
%
20%
219%
424%
205%
%
37%
220%
409%
189%
4%
218%
435%
217%
%
21%
219%
423%
204%
%
38%
220%
408%
188%
5%
218%
435%
217%
%
22%
219%
422%
203%
%
39%
220%
407%
187%
6%
218%
434%
216%
%
23%
219%
421%
202%
%
40%
220%
406%
186%
7%
218%
433%
215%
%
24%
220%
420%
200%
%
41%
220%
405%
185%
8%
218%
433%
215%
%
25#
220#
420#
200#
%
42%
220%
404%
184%
9%
219%
432%
213%
%
26%
220%
419%
199%
%
43%
220%
403%
183%
10%
219%
431%
212%
%
27%
220%
418%
198%
%
44%
220%
402%
182%
11%
219%
430%
211%
%
28%
220%
417%
197%
%
45%
220%
401%
181%
12%
219%
430%
211%
%
29%
220%
416%
196%
%
46%
220%
400%
180%
13%
219%
429%
210%
%
30%
220%
415%
195%
%
47%
220%
399%
179%
14%
219%
428%
209%
%
31%
220%
414%
194%
%
48%
220%
398%
178%
15%
219%
428%
209%
%
32%
220%
413%
193%
%
49%
220%
397%
177%
16%
219%
427%
208%
%
33%
220%
412%
192%
%
50%
220%
396%
176%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
Note:%*%Using%pH/mV%electrode%with%4M%KCL%Ag/Ag%CL%reference%solution%
%
%
%
%
%

VWR sympHony
Meter User Guide
Exhibit 5.2

I
sympHony™Meter User Guide
Table of Contents
Chapter I Introduction
Convenient Meter Features ............................................ 1
Chapter II Display
General Description .................................................. 3
Chapter III Keypad
General Description .................................................. 5
Key Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Chapter IV Preparation
Installing the Power Adapter............................................ 7
Installing the Batteries ................................................ 8
Connecting the Electrodes ............................................. 9
Turning on the Instrument .............................................10
Meter Maintenance ..................................................10
Chapter V Meter Setup
Setup Menu ........................................................11
Setup Menu Table ..................................................12
Enabling or Disabling the Setup Menu Password..........................13
General Menu Settings................................................14
Time and Date Setup .................................................15
AUTO-READ™, Continuous or Timed Measurement Selection ..................16
Selecting the Measurement Parameter ...................................17
Method Setup...................................................... 18
Chapter VI pH Technique
pH Setup Menu .....................................................19
pH Calibration ...................................................... 20
pH Measurement ................................................... 21
mV, Relative mV and ORP Technique .................................... 22

II sympHony™Meter User Guide
Chapter VII Dissolved Oxygen Technique
Dissolved Oxygen Setup Menu ........................................ 23
Dissolved Oxygen Calibration.......................................... 24
Dissolved Oxygen Measurement ....................................... 26
Chapter VIII Conductivity Technique
Conductivity Setup Menu ............................................. 27
Conductivity Calibration .............................................. 28
Conductivity Measurement ........................................... 30
Chapter IX ISE Technique
ISE Setup Menu .................................................... 31
ISE Standards Preparations ........................................... 32
ISE Calibration ..................................................... 33
ISE Measurement................................................... 34
Chapter X Data Archiving and Retrieval
Datalog and Calibration Log ........................................... 35
Chapter XI Declaration of Conformity
Declaration of Conformity............................................. 37
WEEE Compliance .................................................. 38
Chapter XII Troubleshooting
Meter Self Test ..................................................... 39
Meter Error Codes .................................................. 40
General Troubleshooting .............................................. 42
Chapter XIII Meter Specifications
Meter Specifications................................................. 45
Ordering Information ................................................ 48
Appendix A Special Meter Setup Menu Features
pH Setup Menu Features ............................................. 51
Dissolved Oxygen Setup Menu Features ................................. 51
Conductivity Setup Menu Features ..................................... 52
ISE Setup Menu Features............................................. 54
Table of Contents

1
sympHony™Meter User Guide
Chapter I
Introduction
Congratulations! You have selected a VWR®sympHony™meter designed for
electrochemistry measurements in the field or in the laboratory.
• Single parameter meters provide single measurement of pH, dissolved oxygen,
or conductivity.
• Dual parameter meters provide dual measurements of pH/dissolved oxygen,
pH/conductivity, or pH/ISE (ion selective electrode).
• Multiple parameter meters provide multiple measurements of pH, ISE, dissolved
oxygen, and conductivity.
All meters include a temperature measurement function. All meters with pH
measurement capability include a mV/relative mV/ORP function.
Convenient Meter Features
Built to meet the demands of busy, multiple user laboratories or plant environments,
all meters are microprocessor controlled aiding in the delivery of accurate and
precise measurements. To better meet the needs of users in environmental
protection and control, food and beverage, pharmaceutical, and consumer product
laboratories, the sympHony meters include these key features:
• Password Protected Methods – The meter will save up to ten custom
measurements and calibrations for future reference. Password protection of the
setup menu eliminates any tampering with methods as multiple users access
only the procedure most appropriate to their work.
• AUTO-READ™ – The meter will take a measurement and automatically print or
log the data when the reading becomes stable. The measurement is frozen on
the display until the user prompts the meter to take a new measurement.

2sympHony™Meter User Guide
• Stirrer Control – Benchtop meters have a control for the stirrer probe and the
AUTO-STIR™ BOD probe, eliminating the need for additional stir plates and bars.
• Display Backlight – When the meter is on, a quick press of will turn
the backlight on and off. When the meter is operating on battery power, the
backlight will automatically turn off after two minutes to conserve power. When
batteries are low, the backlight will no longer turn on.
• Automatic Shut-off – All sympHony meters will shut down after 20 minutes
without a keypress. This maximizes battery power on portable meters and
benchtop meters that are being run on battery power.
• Audible Signals – The meter will beep whenever a key is pressed, providing
immediate verification that the user’s input was received.
• Visual Alarm Signals – Flashing and icons indicate that calibration
settings need adjustment. For more detail, refer to subsequent sections of this
user guide that discuss specific measurement techniques.
An easy-to-use reference guide, attached to each meter, supports daily meter use.
Note: Please read this user guide thoroughly before using your benchtop or
portable meter. Any use outside of these instructions may invalidate your warranty
and cause permanent damage to the meter.
Introduction

3
sympHony™Meter User Guide
Chapter II
Display
General Description
Throughout a given process, the display on a sympHony meter provides
temperature and calibration data. The temperature appears in the left, top corner
of the display.The icon indicates that a calibration mode or calibration setup
menu is active.The man, 2, 4, 7, 9, 10, and 12 icons indicate which pH buffers
were saved after a pH calibration is performed.The setup icon only appears when
the meter is in setup mode.The icon indicates an error condition and when
it is displayed with the icon, a calibration alarm or sensor quality issue exists.
The icon indicates that the AUTO-READ measurement mode is active and is
discussed in greater detail in the Meter Setup section.
Multi-Parameter Meter
This is the display of the sympHony meter capable of multi-parameter
measurements. The single and dual parameter meters will have fewer
measurement lines, depending on the meter capabilities.
Note: In the measurement mode, the three main lines of data on the meter
display correspond to what is being measured.
Temperature
Calibration
pH/ISE/ORP
Unit
Conductivity
Unit
DO Unit

4sympHony™Meter User Guide
Measurement Unit Icons
In the measurement mode, the arrow icon on the left side of the display screen
indicates the active line.Press to move the arrow icon to the desired line and
press / to scroll through the measurement unit icons associated with
the selected line. The measurement unit icons for the sympHony multi-parameter
meter are shown below. The single and dual parameter meters will have fewer
measurement lines and icons, depending on the meter capabilities.
ApH, mV, relative mV or ISE
BConductivity, TDS, salinity or
resistivity
CDissolved oxygen as % saturation,
dissolved oxygen as concentration,
or barometric pressure
The units of measurement, which are displayed on the right side of the screen, will
flash until the reading is stable.
Note: If a measurement line is not needed, press to move the arrow
icon to the measurement line that is not needed and press / until the
measurement line is completely blank.
Display

5
sympHony™Meter User Guide
Chapter III
Keypad
General Description
The keypad layout is the same for all sympHony meters. The portable meters have
nine keys.The benchtop meters have ten keys due to the addition of the stir key .
Portable Keypad
Benchtop Keypad
setup view log
calibrate measure
save/print
Backlight
Power
Measure
Save/Print
View Log
Line Select
Digits
Setup
Calibrate
Scroll Up
Scroll Down
setup view log
calibrate measure
save/print
Stirrer On/Off
Measure
Save/Print
View Log
Power
Line Select
Digits
Setup
Calibrate
Backlight

6sympHony™Meter User Guide
Key Definitions
Key Description Key Description
Turns the meter on, if the meter
is off.
Toggles the backlight on and off,
if the meter is on.
If the meter is on, hold down
the key for about three seconds
to turn off the meter.
Changes the measurement
units of the selected line in the
measurement mode.
Changes the value on the
selected line in the setup,
methods and log view modes.
Edits the value of the flashing
digit for setup, password entry
and calibration modes.
Scrolls the arrow icon on left of
screen among the three display
lines, so the selected line can
be edited or calibrated.
Selects the next digit to edit
and moves the decimal point
for setup, password entry and
calibration modes.
Starts the calibration for
the selected line in the
measurement mode.
If the arrow icon points to the
top line and the displayed units
are pH, pressing the key will
start a pH calibration.
Each time the key is pressed in
the calibration mode, the meter
will accept the calibration point
and move to the next point
until the maximum number of
calibration points are reached.
Prints and logs a measurement
in the continuous or timed
measurement modes.
Prints, logs and freezes the
display when the reading
becomes stable in the AUTO-
READ measurement mode.
Exits the setup menu and returns
to measurement mode.
Accepts the calibration and
returns to measurement mode.
Enters the setup menu, starting
with selected line in the
measurement mode.
If the arrow icon points to the
top line and the displayed units
are ISE, pressing the key will
enter the ISE setup screen.
Enters the log view and
download menu.
Turns the stirrer on and off.
Keypad