VWR International UV-6300PC User manual

UV-6300PC Spectrophotometer
Instruction Manual
European Catalogue Number:
634-6041
Version: 1.0.2
Issued: 04 April 2014

Legal Address of Manufacturer
Europe
VWR International Europe bvba
Researchpark Haasrode 2020
Geldenaaksebaan 464
B 3001 Leuven
Tel.: [phone]
http://www.be.vwr.com
Made in China

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Table of Contents
Part 1: Spectrophotometer
Safety Information............................................................................................... 3
Package Contents............................................................................................... 4
Unpacking ........................................................................................................... 4
Installation........................................................................................................... 4
Symbols and Conventions .................................................................................. 5
Specifications...................................................................................................... 5
Overview ............................................................................................................. 6
Description of Buttons and Switches................................................................... 6
Operational Keys................................................................................................. 7
Getting Started.................................................................................................... 9
Important Guidelines......................................................................................... 10
General Operating Instructions ......................................................................... 10
Operation .......................................................................................................... 11
Basic Mode.................................................................................................... 12
Quantitative Mode ......................................................................................... 13
Wavelength Scan .......................................................................................... 17
Kinetics.......................................................................................................... 19
DNA/Protein Mode......................................................................................... 22
Multi-Wavelength Mode................................................................................. 23
System Utility................................................................................................. 25
Troubleshooting ................................................................................................ 29
Repair and Maintenance................................................................................... 30
Daily maintenance......................................................................................... 30
Check lamp.................................................................................................... 31
Spare Parts Replacement ............................................................................. 31

2
Accessories and Spare Parts............................................................................ 35
Part 2: Software
Functions........................................................................................................... 37
Main Functions .............................................................................................. 37
Spectrum processing function ....................................................................... 38
System check and calibration function .......................................................... 38
Installation......................................................................................................... 39
Introduction ....................................................................................................... 42
Operation .......................................................................................................... 46
Single wavelength photometric measurement............................................... 46
Fixed point measurement.............................................................................. 47
Wavelength Scanning.................................................................................... 52
Time Scanning (Kinetic Analysis).................................................................. 64
DNA/Protein Measurement............................................................................ 66
Appendix 1 ........................................................................................................ 69
Technical service .............................................................................................. 70
Warranty............................................................................................................ 70
Equipment disposal ........................................................................................... 71

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Part 1: Spectrophotometer
Safety Information
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
VWR issues the following recommendations with regard to the use of the UV-6300PC Spectrophotometer.

Do not open the device.
Disconnect the device from the mains supply before carrying out maintenance work or
changing the fuses.
The inside of the device is a high-voltage area.
Do not use the device if it is damaged, especially if the main power cable is in any way
damaged or defective.
Repairs may only be carried out by the service technicians from your local VWR office
and authorized contractual partners.
The device must be connected to a power outlet that has a protective ground connection.
If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.
Do not allow any liquid to enter the device.
Do not operate the device in a hazardous location or potentially explosive environment.

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Package Contents
Description Quantity
Spectrophotometer 1PC
Glass Cuvette 4PCS
Quartz Cuvette 2PCS
USB Disk 1PC
Power Cord (Euro Plug) 1PC
Power Cord (UK Plug) 1PC
Power Cord (CH Plug) 1PC
USB Cable 1PC
CD-ROM 1PC
Quick Manual 1PC
Instruction Manual 1PC
Dust Cover 1PC
Unpacking
Open the package and carefully check the enclosed items against the packing list. If any of the items are
missing or damaged please contact your local VWR office and authorized contractual partners.
Installation
Placement
Place the instrument carefully on a stable surface.
Install printer (optional)
Check to confirm instrument power switch is turned off, connect the printer’s data cable to the instrument’s
parallel port.
Link the power cord
Check to confirm the instrument power switch is turned off, and the power cord is plugged into two separate
power interfaces and the power supply socket apparatus.

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Symbols and Conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
CAUTION This symbol indicates the presence of high voltage and
alerts you to proceed with caution
CAUTION This symbol indicates risks associated with hot surfaces
Specifications
Optical System Double Beam
Wavelength Range 190–1100 nm
Band Width 1 nm
Stray Light ≤0.05%T @ 220 nm & 360 nm
Photometric Range 0 to 200%T,-0.3 to 3.0 A, 0 to 9999 C
Wavelength Accuracy ±0.3 nm
Photometric Accuracy ±0.3%T or ±0.002 A@1A
Baseline 0.001 A (200 to 1000 nm)
Stability 0.001 A/h @ 500 nm
Memory 32K (internal), Unlimited (USB disk)
Language English, French, German, Spanish
Display 320×240 Dots Matrix LCD
Interface USB, Parallel
Measuring Procedure Photometry, Quantitation, Wavelength Scan,
Kinetics, DNA/Protein, Multi-wavelength
Power Supply AC 110/220 V, 50/60 Hz
Dimension 590×420×260 mm
Weight 26 kg
Work Environment 15 to 35 ℃, 15 to 70% relative humidity
Store Environment -10 to 50 ℃, 15 to 70% relative humidity
This instrument is compliant to the European Directives on
Low Voltage Directive 2006/95/EC
Electromagnetic compatibility 2004/108/EC
Restriction on use of Hazardous Substances RoHS 2011/65/EU and their amendments.

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Overview
UV-6300PC Spectrophotometer used in chemistry, pharmaceuticals, biochemical, metallurgy, light industry,
textile, material, environments, medical, education and some other fields for quality control laboratories.
Description of Buttons and Switches
Front View
Rear View

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Operational Keys
Key Functions
Function keys: Functions on-screen prompts

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… , Numeric keys: Enter numbers and letters
CLEAR key: Delete the input value or stored data
ESC key: Return to previous interface
100%T/0Abs key: Blank
OPEN key: Open files stored in internal memory
SAVE key: Save files to internal memory
START/STOP key: Start/Stop testing
GOTO ʎkey: Set wavelength
PRINT key: Print measuring result
ENTER key: Confirm operation
CELL key: Select/Deselect auto-cell holder
, RIGHT, LEFT keys: Search peak/valley and set X scale
,UP, DOWN keys: Scroll menu/data and set Y scale