VWR Avantor 352 Eco Premium User manual

Version 01 Issue 02/2022
Instruction Manual
VWR®ULT Freezer
352/528 Eco Premium
EU cat. No. 471-1252 / 471-1253 NA cat. No. 76514-174 / 76514-176

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 2/86
Legal address of Manufacturer
Europe
VWR International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-3001 Leuven
[phone]
be.vwr.com
UK Importer
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
uk.vwr.com
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com.
Country of Origin
Germany

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 3/86
Table of Contents
1. INTRODUCTION .................................................................................................... 6
1.1 General guidelines ..............................................................................................................................6
1.2 Operating manual................................................................................................................................ 6
1.3 Legal considerations ...........................................................................................................................6
1.3.1 Compliance with local laws and regulations..............................................................................7
1.3.2 Warranty ....................................................................................................................................7
1.4 Structure of the safety instructions......................................................................................................7
1.4.1 Word message panel structure .................................................................................................7
1.4.2 Warning levels ...........................................................................................................................8
1.4.3 Safety alert symbol ....................................................................................................................8
1.4.4 Warning symbols used in this manual.......................................................................................9
1.4.5 Prohibitory symbols used in this manual ...................................................................................9
1.4.6 Imperative symbols used in this manual ...................................................................................9
1.4.7 Information symbol used in this manual ..................................................................................10
1.5 Localization / position of safety labels at the chamber .....................................................................10
1.6 Type plate..........................................................................................................................................11
1.7 UKCA Importer details ......................................................................................................................12
2. SAFETY................................................................................................................ 12
2.1 Personnel Qualification .....................................................................................................................12
2.2 General safety instructions on installing and operating the chamber ...............................................13
2.3 Intended use .....................................................................................................................................15
2.4 Foreseeable Misuse..........................................................................................................................16
2.5 Residual Risks ..................................................................................................................................17
2.6 Operating instructions by operator....................................................................................................18
2.7 Measures to prevent accidents .........................................................................................................18
3. CHAMBER DESCRIPTION .................................................................................. 19
3.1 Chamber overview ............................................................................................................................20
3.2 Main power switch.............................................................................................................................22
3.3 Chamber rear ....................................................................................................................................23
3.4 Doors.................................................................................................................................................24
3.4.1 Outer door ...............................................................................................................................24
3.4.2 Compartment doors.................................................................................................................24
3.4.3 Controller housing ...................................................................................................................24
3.4.4 Door handle with door lock and eyelets for padlock................................................................25
3.5 Drain well for condensate during defrosting (with deicing kit option)................................................26
4. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 27
4.1 Unloading the VWR®ULT Freezer from the transport pallet ............................................................27
4.2 Unpacking, and checking equipment and completeness of delivery ................................................27
4.3 Guidelines for safe lifting and transportation.....................................................................................28
4.3.1 Moving the freezer inside a building........................................................................................28
4.3.2 Transport outside a building ....................................................................................................29
4.4 Storage..............................................................................................................................................29
4.5 Location of installation and ambient conditions ................................................................................30
5. INSTALLATION AND CONNECTIONS ............................................................... 31
5.1 Operating instructions by operator....................................................................................................31
5.2 Spacers for rear wall distance...........................................................................................................31
5.3 Adjustable shelves ............................................................................................................................31
5.4 Electrical connection .........................................................................................................................34
6. FUNCTIONAL OVERVIEW OF THE CHAMBER CONTROLLER ....................... 35
6.1 Menu structure of the controller and access levels...........................................................................36
7. START UP ............................................................................................................ 37

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 4/86
7.1 Preset factory parameters.................................................................................................................37
7.2 Behavior after turning on the chamber..............................................................................................37
8. TEMPERATURE SET-POINT ENTRY ................................................................. 38
9. PLACING SAMPLES IN STORAGE IN THE FREEZER...................................... 39
10. SETTING SPECIAL CONTROLLER FUNCTIONS .............................................. 40
11. PASSWORD......................................................................................................... 41
11.1 Password request .............................................................................................................................41
11.2 Assign and modify a password .........................................................................................................41
11.2.1 Assign and modify the User password ....................................................................................42
11.2.2 Assign and modify the Admin password .................................................................................42
11.3 Performance during and after power failure and shut down .............................................................43
12. SAFETY CONTROLLER (TEMPERATURE SAFETY DEVICE).......................... 43
12.1 Setting the safety controller mode ....................................................................................................44
12.2 Setting the safety controller value.....................................................................................................44
12.3 Message and measures in the state of alarm ...................................................................................45
12.4 Function check ..................................................................................................................................45
13. GENERAL CONTROLLER SETTINGS................................................................ 46
13.1 Selecting the controller’s menu language .........................................................................................46
13.2 Selecting the temperature unit ..........................................................................................................46
13.3 Setting the current date.....................................................................................................................47
13.4 Setting the current time .....................................................................................................................48
13.5 Function “Language selection at restart” ..........................................................................................48
13.6 Setting the chamber address ............................................................................................................49
13.7 Display brightness.............................................................................................................................49
14. TOLERANCE RANGE AND ALARM DELAY SETTINGS ................................... 50
14.1 Setting the delay time for door open alarm .......................................................................................50
14.2 Setting the delay time for tolerance range alarm ..............................................................................50
14.3 Setting the temperature tolerance range ..........................................................................................51
15. ALARM FUNCTIONS ........................................................................................... 51
15.1 Alarm messages ...............................................................................................................................51
15.2 Information messages.......................................................................................................................53
15.3 Activating / deactivating the audible alarm (alarm buzzer) ...............................................................53
15.4 Required actions in case of an alarm................................................................................................54
15.4.1 Safety controller temperature alarm ........................................................................................54
15.4.2 Temperature tolerance range alarm (too high and too low temperature) ...............................54
15.4.3 Door open alarm......................................................................................................................55
15.4.4 Power failure alarm..................................................................................................................55
15.4.5 Messages referring to temperature sensor failure ..................................................................56
15.5 Zero-voltage relay alarm output ........................................................................................................57
16. ETHERNET NETWORK SETTINGS .................................................................... 58
16.1 Showing the network settings ...........................................................................................................58
16.1.1 Showing the chamber’s MAC address ....................................................................................58
16.1.2 Showing the IP address...........................................................................................................58
16.1.3 Showing the subnet mask .......................................................................................................59
16.1.4 Showing the standard gateway ...............................................................................................59
16.1.5 Showing the DNS server address ...........................................................................................59
16.1.6 Showing the DNS chamber name ...........................................................................................60
16.2 Changing the configuration of the network settings ..........................................................................60
16.2.1 Selecting the type of IP address assignment (automatic / manual) ........................................60
16.2.2 Selecting the type of assignment of the DNS server address (automatic / manual)...............61
16.2.3 Assigning the IP address.........................................................................................................61
16.2.4 Setting the subnet mask ..........................................................................................................62
16.2.5 Setting the standard gateway..................................................................................................62

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 5/86
16.2.6 Assigning the DNS server address .........................................................................................63
17. DATA RECORDER .............................................................................................. 63
17.1 Recorded data...................................................................................................................................63
17.2 Storage capacity ...............................................................................................................................64
17.3 Setting the storage rate for the “DL1” recorder data.........................................................................64
17.4 Deleting the data recorder.................................................................................................................64
18. USB-MENU: DATA TRANSFER VIA USB INTERFACE ..................................... 65
18.1 Connecting the USB stick .................................................................................................................65
18.2 Import function ..................................................................................................................................65
18.3 Export functions ................................................................................................................................66
18.4 Ongoing data transfer .......................................................................................................................66
18.5 Error during data transmission ..........................................................................................................67
18.6 Removing the USB stick ...................................................................................................................67
19. SETTING AND ACTIVATING THE SERVICE SETPOINT ................................... 67
19.1 Setting the service setpoint ...............................................................................................................67
19.2 Activating the service setpoint...........................................................................................................68
20. DATA MONITORING AND RECORDING ............................................................ 68
20.1 Ethernet interface..............................................................................................................................68
21. CHAMBER INVENTORY: STORAGE RACK SYSTEMS AND CRYO BOXES
(OPTION).............................................................................................................. 68
21.1 Storage rack systems with or without cryo boxes .............................................................................68
21.2 Cryo boxes ........................................................................................................................................68
22. CLEANING AND DECONTAMINATION .............................................................. 69
22.1 Cleaning ............................................................................................................................................69
22.2 Decontamination / chemical disinfection...........................................................................................71
23. MAINTENANCE AND SERVICE, SERVICE, TROUBLESHOOTING, REPAIR,
TESTING .............................................................................................................. 72
23.1 General information, personnel qualification.....................................................................................72
23.2 Maintenance intervals, service..........................................................................................................72
23.3 Maintenance work by the user ..........................................................................................................73
23.3.1 Checking and cleaning / replacing the condenser air filter......................................................73
23.3.2 Cleaning the condenser...........................................................................................................74
23.3.3 De-icing and defrosting............................................................................................................74
23.3.4 Maintenance of the door lock ..................................................................................................75
23.4 Simple troubleshooting......................................................................................................................75
23.5 Service Reminder..............................................................................................................................78
24. DECOMMISSIONING ........................................................................................... 78
25. DISPOSAL............................................................................................................ 78
25.1 Disposal of the transport packing......................................................................................................78
25.2 Equipment disposal...........................................................................................................................79
26. TECHNICAL DESCRIPTION................................................................................ 79
26.1 Factory calibration and adjustment ...................................................................................................79
26.2 Over current protection .....................................................................................................................79
26.3 Technical data ...................................................................................................................................79
26.4 Equipment and options (extract) .......................................................................................................81
26.5 User replaceable accessories and spare parts.................................................................................82
26.6 Dimensions of VWR®ULT Freezer 352 Eco Premium .....................................................................83
26.7 Dimensions of VWR®ULT Freezer 528 Eco Premium .....................................................................84
27. CERTIFICATES AND DECLARATIONS OF CONFORMITY............................... 85

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 6/86
1. Introduction
1.1 General guidelines
These operating instructions describe how to set up, operate, clean and adjust the VWR®Ultra-Low Tem-
perature Freezers. They apply to these models:
•VWR®ULT Freezer 352 Eco Premium
•VWR®ULT Freezer 528 Eco Premium
These operating instructions are intended for staff using the equipment in the laboratory.
Other service and maintenance activities should be carried out exclusively by Avantor Services, and are
therefore not explained in this manual.
1.2 Operating manual
This operating manual is part of the components of delivery. Always keep it handy for reference in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
For the correct operation of the VWR®ULT Freezer, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the chamber and/or poor equip-
ment performance.
To avoid injuries and damage observe the safety instructions of the operating manual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the instructions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.
Carefully read the complete operating instructions of the chamber prior to installing
and using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manual is supplemented and updated as needed. Always use the most recent version of
the Operating Manual. When in doubt, call the Avantor Customer Service in your country for information on
the up-to-dateness and validity of this Operating Manual.
1.3 Legal considerations
This operating manual is for informational purposes only. It contains information for correct and safe in-
stalling, start-up, operation, decommissioning, cleaning and maintenance of the product. Note: the contents
and the product described are subject to change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use
and safety during operation and maintenance. Images are to provide basic understanding. They may devi-
ate from the actual version of the chamber. The actual scope of delivery can, due to optional or special
design, or due to recent technical changes, deviate from the information and illustrations in these instruc-
tions this operating manual. In no event shall VWR be held liable for any damages, direct or incidental
arising out of or related to the use of this manual.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 7/86
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please contact the Avantor
Customer Service in your country.
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of VWR derive from the respective purchase contract, which also contains the entire and exclusively
valid statement of warranty administration and the general terms and conditions, as well as the legal regu-
lations valid at the time the contract is concluded. The statements in this manual neither augment nor restrict
the contractual warranty provisions.
1.3.1 Compliance with local laws and regulations
The operator is responsible for applying for and obtaining the necessary regulatory approvals or other au-
thorizations necessary to run or use the product in its local environment. VWR will not be held liable for any
related omission or for not obtaining the required approval or authorization, unless any refusal is due to a
defect of the product.
1.3.2 Warranty
VWR warrants that this product will be free from defects in material and workmanship for a period of two
(2) years from date of delivery plus additional 3 years on the compressor. If a defect is present, VWR will,
at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided
it is returned during the warranty period. This warranty does not apply if the product has been damaged by
accident, abuse, misuse, or misapplication, or from ordinary wear and tear. If the required maintenance
and inspection services are not performed according to the manuals and any local regulations, such war-
ranty turns invalid, except to the extent, the defect of the product is not due to such non-performance.
Items being returned must be insured by the customer against possible damage or loss. This warranty shall
be limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL
BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF
MERCHANTABILITY.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations in ac-
cordance with the standards ISO 3864-2 and ANSI Z535.6.
1.4.1 Word message panel structure
WARNING LEVEL
Warning symbols and/or
Prohibitory symbols and/or
Imperative symbols
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 8/86
1.4.2 Warning levels
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversi-
ble) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury
NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.4.3 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 9/86
1.4.4 Warning symbols used in this manual
Electrical hazard Pollution Hazard
Very cold surface Harmful substances
Explosive atmosphere Biohazard
Stability hazard
Risk of corrosion and / or
chemical burns
1.4.5 Prohibitory symbols used in this manual
Do NOT touch
Do NOT spray with water
Do NOT climb
1.4.6 Imperative symbols used in this manual
Mandatory regulation Environment protection
Read operating instructions Wear protective gloves
Disconnect the power plug Wear safety goggles
Lift with mechanical assistance

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 10/86
1.4.7 Information symbol used in this manual
Information to be observed in order to ensure optimum function of the product
1.5 Localization / position of safety labels at the chamber
The following labels are located on the chamber:
Pictograms (Warning signs) Information
Very cold surface: Risk of freezing Energy Star Symbol
Risk of injury.
Observe the safety instructions in the
operating manual.
Flammable refrigerants
(only UL chambers)
VWR®ULT Freezer, Front VWR®ULT Freezer, rear view
Figure 1: Position of labels on the VWR®ULT Freezer
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact Avantor Services for these replacements.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 11/86
1.6 Type plate
The type plate sticks to the left side of the chamber, bottom right-hand.
Figure 2: Type plate – example VWR ULT Freezer 352 Eco Premium
Figure 3: Type plate – example VWR ULT Freezer 352 Eco Premium-UL
Indications of the type plate (example)
Indication
Information
Avantor by VWR
Manufacturer: VWR
ULT Freezer Eco Premium
Device name of the ultra-low temperature freezer
352 / 528
Model size, referring to the max. quantity of 2” boxes
Serial No.
000000000000
Serial no. of the chamber
Nominal temperature
-86 °C
-123 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880:2006
ECN
471-1252
EU Cat. no. of the chamber
Part no.
76514-174
NA Cat. no. of the chamber
1,60 kW
Nominal power
9,0 A
Nominal current
230 V / 50 Hz
Nominal voltage +/- 10% at the indicated power frequency
1 N ~
Current type
Max. operating pressure 28 bar
Max operating pressure in the refrigerating system
Stage 1: R290 – 0,15 kg
Cooling 1
st
stage: Refrigerant type, filling weight
Stage 2: R170 – 0,15 kg
Cooling 2
nd
stage: Refrigerant type, filling weight
Contains hydrocarbon gases
Contains hydrocarbon gases
Nominal temp.
-86 °C
1,60 kW / 9,0 A
Max. operating pressure 28 bar
-123 °F
230 V / 50 Hz
Stage 1: R290– 0,150 kg
IP protection
20
Stage 2: R170 – 0,150 kg
Safety device
DIN 12880
1 N ~
Contains hydrocarbon gases
be.vwr.com
ECN
471-1252
ULT Freezer 352 Eco Premium
VWR International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-3001
Leuven.
Serial No. 20220000000001
Made in Germany
Nominal temp.
-86 °C
1,40 kW / 13,0 A
Max. operating pressure 28 bar
-123 °F
115 V / 60 Hz
Stage 1: R290– 0,150 kg
IP protection
20
Stage 2: R170 – 0,150 kg
Safety device
DIN 12880
1 N ~
Contains hydrocarbon gases
Part no.
76514-174
ULT Freezer 352 Eco Premium
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
www.vwr.com
Serial No. 20220000000002
Made in Germany

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 12/86
Symbols on the type plate
Symbol Applies to Information
All models CE conformity marking
All models
Electrical and electronic equipment manufactured / placed on
the market in the EU after 13 August 2005 and to be disposed
of in a separate collection according to directive 2012/19/EU on
waste electrical and electronic equipment (WEEE).
UL models only
The chamber is certified by Underwriters Laboratories Inc.®ac-
cording to the following standards:
•UL 61010-1, 3rd Edition, 2012-05, Rev. 2016-04
•UL 61010-2-011, 1st Edition, 2017-01
•CAN/CSA-C22.2 No. 61010-1, 3rd Edition, 2012-
05, Rev.
2016-04
1.7 UKCA Importer details
The sticker with UKCA Importer details sticks next to the type plate to the left side of the chamber, bottom
right-hand.
Figure 4: Sticker with UKCA Importer details
Symbol on the sticker
Symbol Applies to Information
All models except
UL models UKCA conformity marking
2. Safety
2.1 Personnel Qualification
The chamber must only be installed, tested, and started up by personnel qualified for assembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education, knowledge,
experience and knowledge of relevant standards allow them to assess, carry out, and identify any potential
hazards in the work assigned to them. They must have been trained and instructed, and be authorized, to
work on the chamber.
The chamber should only be operated by laboratory personnel especially trained for this purpose and fa-
miliar with all precautionary measures required for working in a laboratory. Observe the national regulations
on minimum age of laboratory personnel.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 13/86
2.2 General safety instructions on installing and operating the chamber
With regard to operating the chamber and to the installation location, please observe the local and national
regulations relevant for your country (for Germany: DGUV guidelines 213-850 on safe working in laborato-
ries, issued by the employers’ liability insurance association).
VWR is only responsible for the safety features of the chamber provided skilled electricians or qualified
personnel authorized by VWR perform all maintenance and repair, and if components relating to chamber
safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original VWR accessories or accessories from third-party suppliers au-
thorized by VWR. The user is responsible for any risk caused by using unauthorized accessories.
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber (chap. 4.5)
NOTICE
Danger to the environment by leakage of refrigerant in the event of a chamber de-
fect.
Alteration of the environment.
Ensure sufficient ventilation of the installation site.
Do not install or operate the chamber in hazardous locations.
DANGER
Danger of explosion due to combustible dusts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP combustible dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Danger of explosion due to introduction of flammable or explosive substances in
the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅Do NOT introduce any combustible dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material.
Familiarize yourself with any potential health risks caused by the charging material. Take adequate
measures to exclude any risk prior to putting the chamber into operation.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 14/86
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber against contamination by toxic, infectious or radioac-
tive substances.
Take appropriate measures when bringing in or taking out toxic, infectious or radioac-
tive substances.
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
∅The chamber must NOT become wet during operation, cleaning, or maintenance.
∅Do NOT install the chamber in damp areas or in puddles.
Set up the chamber in a splash-proof manner.
The chambers were produced in accordance with VDE regulations and were routinely tested in accordance
to VDE 0411-1 (IEC 61010-1).
The inner surfaces become very cold during operation.
CAUTION
Danger of injury by freezing on when touching cold chamber parts during or after
operation.
Local frostbite.
∅Do NOT directly touch the inner surfaces or the charging material during operation.
∅AVOID skin contact with the inner surfaces and accessory equipment.
Wear protective gloves when opening the inner doors and during manipulation.
WARNING
Danger of injury and damages by the chamber tipping over or breakaway of the pro-
truding lower housing cover.
Injuries and damage to the chamber and the charging material
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 15/86
2.3 Intended use
Following the instructions in this operating manual and conducting regular maintenance
work (chap. 22) are part of the intended use.
Any use of the chambers that does not comply with the requirements specified in this Operating
Manual shall be considered improper use.
Other applications than those described in this chapter are not approved.
Use
Ultra-low temperature freezers “VWR®ULT Freezer” are technical equipment and intended solely for use
at work. They are suitable are designed for safe storage of varied materials at temperatures up to -86 °C /
-122.8 °F, especially for long-term storage of biological, medical, and chemical samples at constant low
temperature. They are suitable for the domains Pharmacy, Medicine, Life Sciences, plastic industry, elec-
tronic components, food etc.
Freezers are designed for storage of harmless materials.
Requirements for the chamber load
The charging material shall not contain any corrosive ingredients that may damage the machine compo-
nents made of stainless steel. Such ingredients include in particular acids and halides. Any corrosive dam-
age caused by such ingredients is excluded from liability by VWR.
None of the components of the charging material must be able to form an explosive mixture with air. Any
component of the charging material must NOT be able to release toxic gases.
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion or implosion hazard and danger of poisoning through the introduction of
unsuitable loading material.
Poisoning. Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance combustible or explosive into the chamber, in partic-
ular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
Contamination of the chamber by toxic, infectious or radioactive substances must be prevented
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber against contamination by toxic, infectious or radioac-
tive substances.
Take appropriate measures when bringing in or taking out toxic, infectious or radioac-
tive substances.
In case of foreseeable use of the chamber there is no risk for the user through the integration of the chamber
into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010. For this,
the intended use of the chamber and all its connections must be observed.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 16/86
Medical devices
The chambers are not classified as medical devices as defined by Regulation (EU) No 2017/745.
Personnel Requirements
Only trained personnel with knowledge of the Operating Manual can set up and install the chamber, start it
up, operate, clean, and take it out of operation. Service and repairs call for further technical requirements
(e.g. electrical know-how), as well as knowledge of the service manual.
Installation site requirements
The chambers are designed for setting up inside a building (indoor use).
The requirements described in the Operating Manual for installation site and ambient conditions (Chap.
4.5) must be met.
WARNING: If customer should use a chamber running in non-supervised continuous opera-
tion, we strongly recommend in case of inclusion of irrecoverable specimen or samples to
split such specimen or samples and store them in at least two chambers, if this is feasible.
2.4 Foreseeable Misuse
Other applications than those described in chap. 2.3 are not approved.
This expressly includes the following misuses (the list is not exhaustive), which pose risks despite the in-
herently safe construction and existing technical safety equipment:
•Non-observance of Operating Manual
•Non-observance of information and warnings on the chamber (e.g. control unit messages, safety iden-
tifiers, warning signals)
•Installation, startup, operation, maintenance and repair by untrained, insufficiently qualified, or unau-
thorized personnel
•Missed or delayed maintenance and testing
•Non-observance of traces of wear and tear
•Insertion of materials excluded or not permitted by this Operating Manual.
•Non-compliance with the admissible parameters for processing or storing the respective material.
•Installation, testing, service or repair in the presence of solvents
•Installation of replacement parts and use of accessories and operating resources not specified and au-
thorized by the manufacturer
•Bypassing or changing protective systems, operation of the chamber without the designated protective
systems
•Non-observance of messages regarding cleaning and disinfection of the chamber.
•Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation,
cleaning or maintenance.
•Cleaning activity while the chamber is turned on.
•Operation of the chamber with a damaged housing or damaged power cord
•Continued operation of the chamber during an obvious malfunction
•Insertion of objects, particularly metallic objects, in louvers or other openings or slots on the chamber
•Human error (e.g. insufficient experience, qualification, stress, exhaustion, laziness)
To prevent these and other risks from incorrect operation, it is recommended to issue operating instructions
and Standard operating procedures (SOPs).

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 17/86
2.5 Residual Risks
The unavoidable design features of a chamber, as well as its proper field of application, can also pose risks,
even during correct operation. These residual risks include hazards which, despite the inherently safe de-
sign, existing technical protective equipment, safety precautions and supplementary protective measures,
cannot be ruled out.
Messages on the chamber and in the Operating Manual warn of residual risks. The consequences of these
residual risks and the measures required to prevent them are listed in the Operating Manual. Moreover, the
operator must take measures to minimize hazards from unavoidable residual risks. This includes, in partic-
ular, issuing operating instructions.
The following list summarizes the hazards against which this Operating Manual and the Service Manual
warn, and specifies protective measures at the appropriate spots:
Unpacking, Transport, Installation
•Sliding or tilting the chamber
•Setup of the chamber in unauthorized areas
•Installation of a damaged chamber
•Installation of a chamber with damaged power cord
•Inappropriate site of installation
•Missing protective conductor connection
Normal operation
•Assembly errors
•Contact with cold surfaces on the housing and on the doors
•Emission of non-ionizing radiation from electrical operating resources
•Contact with live parts in normal state
Cleaning and Decontamination
•Penetration of water into the chamber
•Inappropriate cleaning and decontamination agents
•Enclosure of persons in the interior
Malfunction and Damage
•Continued operation of the chamber during an obvious malfunction or outage of the cooling system
•Contact with live parts during error status
Operation of a unit with damaged power cord
Maintenance
•Maintenance work on live parts.
•Execution of maintenance work by untrained/insufficiently qualified personnel
•Electrical safety analysis during annual maintenance not performed
Trouble-shooting and Repairs
•Non-observance of warning messages in the Service Manual
•Trouble-shooting of live parts without specified safety measures
•Absence of a plausibility check to rule out erroneous inscription of electrical components
•Performance of repair work by untrained/insufficiently qualified personnel
•Inappropriate repairs which do not meet the quality standard specified by VWR

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 18/86
•Use of replacement parts other than VWR original replacement parts
•Electrical safety analysis not performed after repairs
2.6 Operating instructions by operator
Depending on the application and location of the chamber, we recommend that the operator of the freezer
provides the relevant information for safe operation of the chamber in a set of operating instructions.
Keep this set of operating instructions with the chamber at all times in a place where they
are clearly visible. They must be comprehensible and written in the language of the employ-
ees.
2.7 Measures to prevent accidents
The operator of the chamber must observe the locally applicable regulations for operating the chamber (for
Germany: Occupational Safety Regulations. Operation of refrigeration chambers, heat pumps and cooling
systems, GUV-R 500 chap. 2.35) and take precautions to prevent accidents.
Following measures have been taken by the manufacturer in order to prevent ignition and explosions:
•Indications of the type plate
See operating manual chap. 1.6
•Operating manual
An operating manual is available for each chamber.
•Temperature monitoring
The chamber has a temperature display which can be read from outside.
An additional temperature safety device is built into the chamber. A visual and an audible signal (buzzer)
show exceeding of the temperature.
•Safety, measurement and control devices
The safety, measuring, and control devices are easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electrical
equipment (e.g. electric motors). The machine has strong permanent magnets. If persons with active
implants (e.g. pacemakers, defibrillators) keep a safe distance (distance of field source to implant) of 30
cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-3:2008.
•Floors
See operating manual chap. 4.5 for installation
•Cleaning
See operating manual chap. 22.
•Examinations
UL chambers only: The chamber is certified by Underwriters Laboratories Inc.®according to the stand-
ards UL 61010-1, 3rd Edition, 2012-05, Rev. 2016-04; UL 61010-2-011, 1st Edition, 2017-01; CAN/CSA-
C22.2 No. 61010-1, 3rd Edition, 2012-05, Rev. 2016-04

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 19/86
3. Chamber description
The VWR®ULT Freezers were produced with great care using the latest tools for development and pro-
duction. They were optimized for safe long-term storage of samples in the ultra-low temperature range. You
can operate the freezer in a temperature range from -86 °C / -122.8 °F up to -50 °C / -58 °F.
The freezers are available for several different voltages.
Lockable main switch (option)
An additional locking system with key for the freezer’s main power switch is optionally available.
Controller
The efficient chamber controller is equipped with a multitude of operating functions, in addition to recorder
and alarm functions. Set-point entry is easily accomplished directly via the chamber controller. Temperature
setting is accurate to a tenth of a degree. The controller is mounted at the optimal height for operation.
The controller offers an error diagnostics system generating audible and visual warning and alarm mes-
sages. The controller provides password protection for the setting menus.
The controller monitors ambient temperature and issues an alarm if it exceeds an adjustable value.
Housing
The inner chamber and the inside of the insulated outer door are made of stainless steel (German material
no. 1.4016, US equivalent AISI 430). The housing including all corners and edges is RAL 7035 varnished.
The inner surfaces are smooth and therefore easy to clean. Easy front access permits filter cleaning without
tools. Two 28 mm access port serve to introduce a sensor cable of a measuring device.
The buildup of ice in the door area is minimal due to perfect closing of the inner and outer doors. Precise
spatial distribution of the cold in the interior ensures storage of all samples at an identical storage temper-
ature. The prevention of thermal bridges protects against defrosting. The combination of vacuum insulation
panels (V technology) and CFC-free polyurethane foaming maximizes the cold storage capacity.
The freezer has two compartment doors. You can insert stainless steel shelves are make optimum use of
the interior. You can flexibly arrange the shelves to use the interior in a variable and optimum manner.
Inventory racks (stainless steel storage racks with cryo boxes, chap. 21) are optionally available.
Castors with locks serve to move the freezer.
Cooling system
The powerful, energy-efficient and low-noise refrigerating machine uses the environmentally friendly
“green” refrigerants R290 (propane) und R170 (ethane). They are completely free of HCFCs (hydrochloro-
fluorocarbons) and CFCs (chlorofluorocarbon).
Control of the two-stage refrigerating machine: The 1st stage cooling immediately turns on. In addition, the
2nd stage cooling turns on depending on the temperature.
Safety
Thanks to the standard overtemperature safety device, the set temperature is maintained also in case of a
controller failure.
In case of power failure at -80 °C / -112 °F, a temperature of -60 °C / -76 °F will not be exceeded in an
empty freezer for at least 3.5 hours, in a loaded freezer (measured with a 30 kg / 66 lb water load) for
approx. 7 hours.
Data monitoring and recording
The chamber is regularly equipped with a zero-voltage relay alarm output (chap. 15.5) for integration into
customer systems.
The freezer is regularly equipped with an Ethernet interface (chap. 20.1) for computer communication,
enabling monitoring via a network.

vwr.com I Instruction manual VWR®ULT Freezer 352/528 Eco Premium 02/2022 Page 20/86
3.1 Chamber overview
(A)
(B)
(C)
(D)
(E)
(F)
Figure 5: VWR®ULT Freezer 528 Eco Premium, front view
(A) Outer door
(B) Controller housing
(C) Door handle
(D) Compressor housing
(E) Air filter flap (checking and cleaning / replacing the filter chap. 23.3.1)
(F) Castors (front castors lockable by breaks)