VWR Avantor 352 Eco Premium Product information sheet

Version 01 Issue 02/2022
Short user’s manual
VWR®ULT Freezer
352/528 Eco Premium
EU cat. No. 471-1252 / 471-1253 NA cat. No. 76514-174 / 76514-176
Important note
This Short user’s manual is only valid in conjunction with the operating manual. You can download it
under www.vwr.com or contact your local VWR office.
The initial commissioning and usage of the VWR®ULT Freezer (during the entire product life cycle) is
only permitted with the operating manual. Read the operating manual completely before usage.
This Short user’s manual is not a replacement for the operating manual, as it only contains selected
information.

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Safety instructions
The following represents the hazards with the highest level of danger for people. Safety information with lower risk
levels, and important detailed information about safety, can be found in the operating manual. It is very important
that the operating manual is read completely before initial commissioning and usage.
The VWR®ULT Freezer should only be operated by laboratory personnel especially trained for this purpose and
familiar with all precautionary measures required for working in a laboratory. Observe the national regulations on
minimum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the operat-
ing manual.
DANGER
Danger of explosion due to combustible dusts or explosive mixtures in the vicinity of the
chamber or inside the chamber.
Serious injury or death from burns and / or explosion pressure.
Ø Do NOT operate the chamber in potentially explosive areas.
Ø KEEP explosive dust or air-solvent mixtures AWAY from the chamber.
Ø Do NOT introduce any substance combustible or explosive at working temperature into the
chamber, in particular no energy sources such as batteries or lithium-ion batteries.
Ø NO explosive dust or air-solvent mixture in the inner chamber.
DANGER
Electrical hazard during live maintenance work.
Deadly electric shock.
Ø The chamber must NOT become wet during operation or maintenance.
Ø Do NOT remove the rear panel of the chamber.
Disconnect the chamber before cleaning or conducting maintenance work. Turn off the main
power switch and pull the power plug.
Ensure all maintenance work is conducted by licensed electricians or experts authorized by the
manufacturer.
WARNING
Danger of intoxication and infection through contamination of the chamber with toxic, infec-
tious or radioactive substances.
Damages to health.
Protect the interior of the chamber against contamination by toxic, infectious or radioactive sub-
stances.
Take appropriate measures when bringing in or taking out toxic, infectious or radioactive sub-
stances.
Ø Do NOT introduce any substance which could lead to release of toxic gases

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CAUTION
Danger of injury by freezing on when touching cold chamber parts during or after operation.
Local frostbite.
Ø Do NOT directly touch the inner surfaces or the charging material during operation.
Ø AVOID skin contact with the inner surfaces and accessory equipment.
Wear protective gloves when opening the inner doors and during manipulation.
WARNING
Danger of injury and damages by the chamber tipping over or breakaway of the protruding
lower housing cover.
Injuries and damage to the chamber and the charging material
Ø Do NOT climb on the lower housing cover.
Ø Do NOT load the lower housing cover with heavy objects while the chamber door is open.
Intended use
Ultra-low temperature freezers “VWR®ULT Freezer” are technical equipment and intended solely for use at work.
They are suitable are designed for safe storage of varied materials at temperatures up to -86 °C / -122.8 °F, espe-
cially for long-term storage of biological, medical, and chemical samples at constant low temperature. They are
suitable for the domains Pharmacy, Medicine, Life Sciences, plastic industry, electronic components, food etc. The
freezers do not dispose of any measures of explosion protection
The charging material shall not contain any corrosive ingredients that may damage the machine components made
of stainless steel. Such ingredients include in particular acids and halides. Any corrosive damage caused by such
ingredients is excluded from liability by VWR. None of the components of the charging material must be able to
form an explosive mixture with air. Any component of the charging material must NOT be able to release toxic
gases.
Other applications are NOT approved.
Details can be found in the operating manual in chapter 2.3. Any use of the chambers that does not comply with
the requirements specified in the Operating Manual shall be considered improper use. Following the instructions in
the operating manual and conducting regular maintenance work are part of the intended use.
You may not make any modifications to the device yourself, as these may limit proper usage.
The chamber must only be operated using only original accessories or accessories / components
from third-party suppliers authorized by the manufacturer. The user is responsible for any risk arising
from using unauthorized accessories
VWR is responsible for the safety features of the chamber only, provided skilled electricians or quali-
fied personnel authorized by the manufacturer perform all maintenance and repair, and if compo-
nents relating to chamber safety are replaced in the event of failure with original spare parts. The
user is responsible for any risks arising from using unauthorized accessories/components.

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Components
(A)
(B)
(C)
(D)
(E)
(F)
(L1)
(L2)
(M)
(N)
Front view Rear view
(A) Outer door
(B) Controller housing
(C) Door handle
(D) Compressor housing
(E) Air filter flap for checking and cleaning /
replacing the filter
(F) Castors (front castors lockable by breaks)
(L1) 28 mm access port, e.g., for cable of a supple-
mentary measuring device
(L2) 28 mm access port, e.g., for cable of a supple-
mentary measuring device
(M) Connection panel with Ethernet interface
(N) Connecting socket for IEC connector plug with
strain relief

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View with open door
(A) Outer door
(C) Door handle
(D) Compressor housing
(E) Air filter flap for checking and cleaning / replacing the filter
(F) Castors (front castors lockable by breaks)
(G) Compartment with variable shelf
(H) Compartment door
(I) Pressure compensation valve (inside of the door behind the door lock and controller housing)
(A)
(H)
(C)
(I)
(G)
(
H)
(D)
(E)
(F)

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Controller overview
Normal display of the chamber controller (sample values)
Controller buttons
Status icons
Temperature value
Text information
Temperature
Status icons in the controller display
Icon
Signification
Icon
Signification
Door open Collective alarm
Do not open the door Refrigeration active
Information
Display of activated special controller functions.
3 = Service setpoint active
Functional controller keys
Icon
Signification
Function
Arrow-up button
•Navigate between menus, submenus, other functions
•In the setting menu: change setting, increase value
Arrow-down button
•Navigate between menus, submenus, other functions
•In the setting menu: change setting, decrease value
OK button
•Select menu, submenu, function
•In the setting menu: Confirm entry
Back button Back to previous menu level
Standby button no function

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Lifting and transporting the chamber
Inside a building on a smooth floor: Use castors
•Unlock the front castors before moving the freezer
•Angle of inclination less than 5°: Wait 10 minutes before turning on the freezer again
•Angle of inclination: 5° to 10° (maximum!): Wait at least 8 hours before turning on the freezer again
Outside a building: Use technical equipment
•Do NOT lift or transport the chamber using the door, the door handle, the controller housing or the lower housing.
•If possible, avoid transporting the chamber horizontally. It may be transported lying down ONLY on the hinge side
or on its back, but must then stand upright for at least 24 hours before turning on
•Lift the chamber using technical devices (fork lifter) and place it on the transport pallet. Set the fork lifter laterally
or from the rear in the middle of the chamber. Make sure to place all the lateral supports of the chamber on the
forks (check: the fork protrudes at the opposite chamber side).
•Transport chambers ONLY with the original transport pallet. Set the fork lifter only to the pallet. Without the pallet
the chamber is in imminent danger of overturning
•Wear suitable shoes (safety shoes)..
•Transport the chamber only in its original packaging.
•Secure the chamber with transport straps for transport.
Requirements for the location of installation
•Installation inside a building on a non-flammable, flat and even surface, free from vibration
•Do NOT set up chambers in non-ventilated recesses. Ensure sufficient ventilation for dispersal of the heat.
•Permissible ambient temperature range during operation: +18 °C up to +32 °C / 89.6 °F.
•Permissible ambient humidity: 70 % r.h. max., non-condensing
•Installation height: max. 2000 m / 6562 ft. above sea level.
•Do not install or operate the chamber in potentially explosive areas.
•Ensure a distance of at least 100 mm / 3.94 in to the ventilation openings on the freezer’s front and rear.
•Power supply fluctuations must not exceed +/- 10 % of the nominal voltage
Electrical connection data and installation
•The chambers come with an IEC connector plug. Cable length: 2000 mm / 78.7 in. Only use original connection
cables according to the specification given in the Operating Manual.
•Pollution degree (acc. to IEC 61010-1): 2. Over-voltage category (acc. to IEC 61010-1): II. Housing protection
(acc. to 60529): IP 20
•The domestic socket must also provide a protective conductor. Make sure that the connection of the protective
conductor of the domestic installations to the chamber’s protective conductor meets the latest technology. The
protective conductors of the socket and plug must be compatible!
•When connecting, please observe the regulations specified by the local electricity supply company as well as the
local or national electrical regulations. We recommend the use of a residual current circuit breaker.
•Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located
on the chamber’s type plate.
•To completely separate the chamber from the power supply, you must disconnect the power plug. Install the
chamber in a way that the power plug is easily accessible and can be easily pulled in case of danger.

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Chamber size (referring to the max. quantity of 2” boxes)
352
528
VWR®ULT Freezer (230 V)
Nominal voltage (+/-10%) at 50 Hz power frequency
V
230
230
Current type
1N~
1N~
Nominal power
kW
1.6
1.6
Nominal current
A
7.0
7.0
Power plug
Grounded plug EU, UK, CH
Internal over-current release category C, 2 poles
A
10
10
VWR®ULT Freezer, UL model (120 V)
Nominal voltage (+/- 10%) at 60 Hz power frequency
V
115
115
Current type
1N~
1N~
Nominal power
kW
1.4
1.4
Nominal current
A
11.7
11.7
Power plug
NEMA
5-15 P
5-15 P
Internal over-current release category C, 2 poles
A
13
13
Placing samples in storage in the freezer
•Load the freezer only after equilibration of temperature
•After turning on the chamber, wait approx. 8 hours, before place any samples in the freezer
•Only insert samples into the freezer when it has reached its stable operating state.
•Do NOT directly touch the inner surfaces or the charging material during operation.
•AVOID skin contact with the inner surfaces and accessory equipment.
•Wear protective gloves when opening the inner doors and during manipulation
•After the outer door has been closed, it can only be opened again after a waiting time
Temperature equilibrating time to -80 °C / -112 °F :
Approx. 6 hours (VWR®ULT Freezer 352) / approx. 7.5 hours (VWR®ULT Freezer 528).
Waiting time after the outer door has been closed
Time displayed as a countdown.
“Information” icon is lit
“Do not open the door” icon flashes.
Normal display during the waiting time
(example image)
Wait 56s

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Preset factory parameters
•Temperature set point -80 °C / -112 °F
•Safety controller mode “Limit”
•Safety controller value -50 °C / -58 °F
•Deviation from the temperature set-point causing tolerance range alarm +/- 5 K
•Alarm delay time after opening the door 1 minute
•Alarm delay time after leaving the tolerance range 60 minutes
•Password for “User” authorization 0 (no locking)
•Password for “Admin” authorization 1
Temperature setpoint entry
Normal Display (example image)
Temperature
Change to Setpoints menu
Change to Temperature submenu
Enable setting
Enter the set-point.
Setting and control range: -50 ºC / -58 °F up to -86 °C / -122.8 °F
Temperature
Confirm the entry
Go back to Normal Display
Setting the safety controller
•Regularly check the settings of the safety controller mode and value.
•Set the safety controller value by approx. 15 °C above the desired temperature set-point.
•Check and/or adjust the safety controller following any change of the temperature set-point

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First step: Setting the safety controller mode
Normal Display (example image)
Temperature
Change to Setpoints menu
Change to Safety controller menu
Change to Mode menu
Enable setting
Select the mode LIMI (Limit) or
OFFS (Offset)
Mode
Mode
Confirm the entry
Proceed to next step
Next step: Setting the safety controller value
Depending on the mode setting, one of the following setting menus will appear.
Enter the desired limit value. Setting range: -20 °C to -110 °C.
(example image)
Limit
Or
Enter the desired offset value.
(example image)
Offset
Confirm the entry
Go back to Normal Display

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Troubleshooting in the event of overtemperature alarm
Overtemperature alarm (example image)
Safety controller
•Check whether the outer door was open for a long time or is not closed properly.
•Check the setting of the safety controller. If necessary, adjust the relevant value.
•Check whether samples were inserted into the freezer that may release heat.
•Check the ambient conditions. Protect the freezer from direct sunlight. Ensure sufficient ventilation.
•If these points do not reveal the source of the fault, it may be that the chamber is faulty. Please contact Avantor
Services.
Cleaning and decontamination
With every cleaning method, always use adequate personal safety controls.
Clean the door handle, door hinges and the locking counterpart at least once a year.
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
Ø Do NOT spill water or cleaning agents over the inner and outer surfaces.
Ø Do NOT put ANY cleaning aids (cloth or brush) into slots or openings on the cham-
ber.
Before cleaning, turn off the chamber at the main power switch and disconnect the
power plug.
Completely dry the chamber before turning it on again.
DANGER
Risk of locking in a person.
Death from suffocation or freezing.
Before closing doors, make sure that nobody is inside.
Pull the power plug before entering the interior (e.g. for cleaning purposes).
CAUTION
Danger of chemical burns through contact with skin or ingestion of the neutral
cleaning agent and through eye contact with the disinfectant spray
Skin and eye damage.
Ø Do not ingest the neutral cleaning agent. Keep it away from food and beverages.
Wear protective gloves (butyl or nitrile rubber, penetration time >480 minutes) and
sealed protective goggles.
Avoid skin contact with the neutral cleaning agent.

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•For cleaning, wipe the surfaces with a moistened towel or use cleaning agents and disinfectants.
•Do not use cleaning or decontamination agents that may cause a hazard due to reaction with components of the
freezer or the loading material. If there is doubt regarding the suitability of cleaning products, please contact
Avantor Services.
•For surface protection, perform cleaning as quickly as possible.
•After cleaning completely remove cleaning agents from the surfaces with a moistened towel. Allow the chamber
to dry.
•Following use of disinfectants: allow the chamber to dry, and aerate it sufficiently.
Suitable agents for cleaning
Exterior surfaces, door handle, controller
housing with controller panel, interior
(stainless steel) shelves, door gaskets
Standard commercial cleaning detergents free from acid or halides.
Alcohol-based solutions.
Recommended for cleaning: neutral cleaning agent
Instrument panel Standard commercial cleaning detergents free from acid or halides.
Recommended for cleaning: neutral cleaning agent
Zinc coated hinge parts
rear chamber wall
Standard commercial cleaning detergents free from acid or halides.
Do NOT use a neutral cleaning agent on zinc coated surfaces.
Suitable agents for disinfection
Interior (stainless steel) Standard commercial surface disinfectants free from acid or halides (not dripping).
Alcohol based solutions.
Compartment doors Standard commercial surface disinfectants free from acid or halides (not dripping).
Alcohol based solutions max. 10%
Outer door gasket (PVC) and
inner door gasket (silicon) Alcohol based solutions
Maintenance
The following work must be carried out regularly by the operating personnel to maintain the chamber function
De-icing the upper area of the freezer and the interior doors.
•Regularly – e.g. every month – remove the frost on the doors. Use the supplied ice scraper (part of optional
deicing kit).
•NEVER use tools with a sharp edge to remove the frost.
•If the door has not been opened for more than 5 days, de-ice the door gaskets and the inner opening of the
pressure compensation valve.
Defrosting the entire chamber
•Before defrosting, turn off the empty chamber and disconnect it from the power supply. Open the outer door and
all inner doors.
•Place absorbent towels on the bottom of the inner chamber or mount the optional drain well, and allow the frost
to melt. Wipe up the accumulated water with absorbent towels
•Let the interior of the freezer dry. Clean and decontaminate it and let it dry before connecting it to the power supply
and turning it on

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Cleaning the condenser
•Every 6 months remove by suction any visible dust on the condenser lamellas with a vacuum cleaner.
•If appropriate, blow through the lamellas with compressed air.
With an increased amount of dust in the ambient air:
•Clean the condenser several times a year.
•Weekly check the condenser lamellas behind the air filter flap.
•If soiling is visible, turn off the chamber and remove the dust by suction from the condenser lamellas.
Checking and cleaning / replacing the condenser air filter
•Check the condenser air filter visually for soiling every month and/or when the alarm message “Condenser temp.”
occurs.
•The filter is located behind the air filter flap in the lower housing cover. rinse the filter and use it again
•To access the condenser air filter (E1), unscrew the quick locking screws (E2) of the air filter flap (E) and re-
move the air filter flap.
•For reuse, wash the condenser air filter with water and let it dry. If necessary, replace the filter.
•Insert the condenser air filter and mount the air filter flap. Mount the quick locking screws.
(E2)
(E2)
(E1)
(E)
Maintenance of the door lock
•Clean the door handle, door hinges and the locking counterpart at least once a year.
•After cleaning, lubricate the running surface of the handle and the locking counterpart with medical vaseline.