WPI UltraMicroPump II User manual

UltraMicroPump II
UMP2 Microsyringe Injector and Micro4 Controller
INSTRUCTION MANUAL
Serial No._____________________
071807
World Precision Instruments, Inc.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 31
Copyright © 2006 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
Contents
Introduction ...................................................................................... 1
Parts List............................................................................................ 2
Set-up and Operation........................................................................ 2
Setting Parameters............................................................................ 6
UltraMicroPump II Operation........................................................... 13
Defining Other Syringes .................................................................. 14
Computer Control ............................................................................ 18
General Maintenance...................................................................... 25
Troubleshooting............................................................................... 25
Specifications.................................................................................. 22
Syringes........................................................................................... 23
Accessories, Replacement Parts .................................................... 24
References ...................................................................................... 27
Appendix: Nanoliter 2000 / Micro4 Volume Settings....................... 28
Warranty .......................................................................................... 28

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 1
Introduction
Thank you for purchasing WPI's UltraMicroPump II (UMP2) and the
microprocessor controller, Micro4™(UMC4). This versatile injector uses
microsyringes to dispense nanoliter sample volumes. Microsyringes are easily
installed by placing the syringe barrel into the UltraMicroPump’s clamps.
UltraMicroPump accepts syringes from 0.5 µL to 250 µL.
UltraMicroPump can be useful for a wide range of applications including
intracellular injection, micro delivery of biochemical agents or dyes, cell
separation, in vitro fertilization, and can be mounted directly onto a stereotaxic
frame or micromanipulator.
Fig. 1 — UltraMicroPump II and Micro4 controller. The pump above
is mounted on WPI’s M3301 micromanipulator and TB-1 stand (not
included); and syringe with luer µTip (not included). At left, pump is
shown with TAXIC900 stereotaxic frame (not included).

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 2
Operating parameters for the UltraMicroPump are set with the Micro4. Up to four
pumps may be independently controlled. Operating parameters set by the user are
stored in “non-volatile” memory for instant recall when the unit is powered on.
An optional footswitch can be plugged into an RS232 port on the rear of the
controller for “hands free” start / stop operation. The same port may also be used to
connect the controller to a computer or to some other device for TTL triggering.
Parts List
After unpacking, make certain that there is no visible damage to the instrument.
Check to see that all items are included:
UMP2 UltraMicroPump II
UMC4 Micro4™Controller, 4-Channel
40300 12-volt Power Supply
Power Cable
Allen key
Instruction Manual
For a list of microsyringes available from WPI, see page 23.
Set-up and Operation
These instructions will help you put the UltraMicroPump to immediate use. We
recommend that you read the entire manual and familiarize yourself with the
various operating procedures of the UltraMicroPump and Micro4.
Mounting the syringe
Syringes may be filled manually before mounting in the UltraMicroPump or filled by
using the fast reverse function (see page 13). Place the plunger button of the filled
syringe into the plunger button holder (leaving the plunger retaining screw loose)
while also placing the syringe collar into the collar stop (see Fig. 2). Take care not
to damage the syringe collar during this installation. Gently tighten the plunger
retaining screw so that the plunger button will not move independently when the
pump is activated (this allows for zero volume error during pump operation).

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 3
Fig. 2 — Initial syringe clamp adjustment — Secure syringe by sequentially
adjusting set screws with supplied Allen key: 1for horizontal alignment, 2for
vertical alignment, and 3to eliminate play.
Axial Needle Alignment
In order to maintain a good syringe needle alignment particularly along the same
axis of the supporting bar, rotate the syringe body while placing in the two clamps
to allow the syringe to seat properly and align along the body of the pump for
minimal slant offset. If the clamp which holds the syringe collar (see “collar stop”
in the drawing below) is too tight or too loose, syringe needle alignment may be off
because of stress.
If the collar stop is too tight to allow the syringe collar to insert easily, then follow
these instructions:
Immediately below and behind the collar stop, in the groove where the long drive
screw is, there is a small Phillips head screw, attached to the collar stop. Loosen
this screw to slightly (about 0.5 to 1 mm) to allow for a thicker collar (see Fig 2).
You may have to grasp the collar stop and wiggle it backwards to move it. Once
the stop is backed out; check and adjust for a tight fit so the syringe body doesn’t
move when placed into the holder. Gently re-tighten the screw in the new position.
Note: you may have to retract the plunger button holder to get access to the set-
screw.
Mount the pump
The UltraMicroPump can
be mounted directly onto
a stereotaxic frame or a
micromanipulator, using
the mounting bar (see
Fig. 2). The mounting bar
may be unscrewed and
modified by the user, if
necessary.
MOUNTING BAR
COLLAR
STOP
SYRINGE
COLLAR
SET SCREW
(not visible in this view)
PLUNGER
RETAINING
SCREW
PLUNGER BUTTON
PLUNGER
BUTTON
HOLDER
CABLE CONNECTS
TO REAR PANEL OF
MICRO4 CONTROLLER
SYRINGE
CLAMPS
CLAMP
RELEASE
SET
SCREWS
SYRINGE
1
2
3

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 4
Footswitch or RS232 cable from
computer plugs into this port.
Connect up to four
UltraMicroPumps.
SWITCH
FOOT
RS232/
+12VDC IN
4
32
POWER ON
1
OFF
OUTPUT
Fig. 3 — Micro4
rear panel
Plug in the 12-volt power
supply.
Mounting to Stereotaxic Frames
WPI’s UMP2 fits directly into most standard stereotaxic frames. The UMP2
mounting bar diameter is 7.90 mm (0.311 in.). For example, UMP2 fits
directly into Kopf Standard 900 series frames (in place of 1770 electrode
holder). Stoelting #51681 V-clamp adaptor / probe holder attachment (WPI
#502201, at left) required for use with Stoelting frames.
Connect the controller
Plug the UltraMicroPump cable into an output socket on the back of the Micro4
controller (see Fig. 3). Up to four pumps may be connected and independently
controlled.
Plug in the 12-volt power supply and power up
Plug the 12-volt power supply into the rear panel of the Micro4 (see Fig. 3). The
switchable power supply included with this unit automatically senses input line
voltage between 100 and 240 V and converts it to 12 V. Connect the power cord
to the power supply and plug it into an electrical outlet. The power switch is also
located on the rear panel of the Micro4.
Switch the Micro4 on and check that the LCD screen is illuminated.
The hardware setup is now complete. Before operating the UltraMicroPump you
must enter the parameters into the Micro4 controller.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 5
Choosing a Syringe
The syringe should be chosen to inject no less than 5% of the volume of any given
syringe.
Example: A 100 µL syringe may be used for injections on the UMPII to volumes of
5 µL (5000 nL) and higher with high precision and repeatability. Expecting this 100
µL syringe to inject lower than 1 µL may be difficult if the syringe is not calibrated
specifically on the pump. The overall accuracy of the syringe itself is usually no
greater than +/-3% and the syringe internal diameter may deviate from location to
location along the length of the syringe interior.
Choosing the correct syringe for an injection is a very important consideration,
due to accumulated errors of the syringe and the pumping method. For a 1000 nL
injection from a 10 µL (10,000 nL) syringe, the user is asking for a 1/10th the
syringe’s volume value to be injected. Theoretically, based on mathematics without
any consideration to surface tension, heat, pressure, compressibility, silanization,
or air bubbles, the 3% error rate should yield a ± 300 nL variance.
As a rule of thumb, the choice of the syringe should not exceed 1/10th to 1/20th the
stated full volume of the syringe. The Micro4-UMP2 system uses a stepper motor
to move the syringe piston forward to inject the volume. A consideration should
also be made to allow the motor to step forward a good number of steps to prevent
errors in volume injecting.
Example: In the 10 µL case above, a 1-step movement of the motor will inject
a volume of 0.5276 nL. For two steps to occur, a volume of 1.055 nL will be
injected. This may or may not be acceptable as the total error may exceed 1
nL or nearly 0.1%. Also note that two steps is probably not enough resolution to
accurately control the volume; another rule of thumb would be to step no less than
10-100 steps for the entire single injection. Using this 10-step rule, the minimum
acceptable injectable volume from this 10 µL (10,000 nL) syringe would be
0.527.6 nL × 10 or 5.276 nL. Other syringes will give other results, due to the inside
diameter and the volume per step.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 6
G = Grouped
N = Non-grouped
D = Disabled
World Precision
Instruments
MicroSyringe Pump Controller
Output 1
Output 2
Output 3
Output 4
VOLUME
SET
(nl)
VOLUME
COUNTER
(nl)
RATE
DEVICE TYPE
123
456
789
.
0
SELECT
DATA
SAVE
RUN
STOP
I=Infuse
W=Withdraw
TM
S=nl/sec
M=nl/min
Arrow keys:
Move the cursor
around the display.
Numeric keypad: Use these keys to
enter settings for infusion and withdrawal
rates and volumes.
Select: Toggles
the selected
parameters: Infuse/
Withdraw, Syringe
Size, Rate Units
(nL/sec or nL/min),
and mode (Grouped,
Non-grouped and
Disabled).
Run/Stop: Starts
and stops the
pump(s).
Data Save: Saves all parameters on LCD display
to memory. Next time the Ultra Microsyringe Pump
controller is turned on, the last paramaters saved will
be displayed.
Lights: When a
pump is running,
the correspond-
ing light is on. For
example, the light
next to output 1 will
be on when pump
one is operating.
Fig. 4 — Micro4
front panel
Setting Parameters
Parameters are entered using the membrane keys on the front panel of the Micro4.
The l e f t and r i g h t a r r o w keys move the cursor on the LCD display to the desired
position. The u p and d o w n a r r o w keys select the channel (corresponding to the
output channels on the rear of the instrument). The n u m b e r keys and the s e l e c t
key are used to change parameters.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 7
Setting Infuse/Withdraw
The character displayed in the first field indicates the operating mode — Ifor
Infuse, Wfor Withdraw. To change modes, use the a r r o w k e y s to position the
cursor in this field, then press s e l e c t .
I = Infuse
W = Withdraw
# = Option
In addition, the options below may be enabled or disabled when the cursor is in
the Infuse/Withdraw field: position the cursor in the Infuse/Withdraw field and press
the corresponding option number.
Disables audible tone.
Enables audible tone.
Changes the action of r u n /s t o p key or footswitch. When this option is
enabled the pump will operate as long as the r u n /s t o p key or footswitch is
pressed and stop when the r u n /s t o p key or footswitch is released.
Returns r u n /s t o p key or footswitch to normal operation.
Sets Volume per Step for syringe types not already preset in the Micro4’s
memory (see page 14).

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 8
Volume Set
To select the desired volume to be infused or withdrawn, position the cursor in
the volume set field and enter the numbers with the numeric keypad. The values
shown on the LCD display are in nanoliters. (Note: For 10 µL enter “10000”; for
1.0 µL enter “1000.”—including decimal point.)
Syringe Injection Accuracy: Since every syringe in the microliter volume range
has its own unique intricacies, you should verify and calibrate each syringe and log
its characteristics for accurate injections. The Micro4 controller has preset types
of syringes to very accurately move the plunger button of the syringe a precise
distance per injection.
VOLUME
SET
(nL)
Volume Counter
Real-time display of volume being delivered. When pump is not running, this
number may be changed; when pump is restarted, counter will continue from the
number entered.
VOLUME
COUNTER
(nL)