WPI UltraMicroPump II User manual

UltraMicroPump II
UMP2 Microsyringe Injector and Micro4 Controller
INSTRUCTION MANUAL
Serial No._____________________
071807
World Precision Instruments, Inc.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 31
Copyright © 2006 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
Contents
Introduction ...................................................................................... 1
Parts List............................................................................................ 2
Set-up and Operation........................................................................ 2
Setting Parameters............................................................................ 6
UltraMicroPump II Operation........................................................... 13
Defining Other Syringes .................................................................. 14
Computer Control ............................................................................ 18
General Maintenance...................................................................... 25
Troubleshooting............................................................................... 25
Specifications.................................................................................. 22
Syringes........................................................................................... 23
Accessories, Replacement Parts .................................................... 24
References ...................................................................................... 27
Appendix: Nanoliter 2000 / Micro4 Volume Settings....................... 28
Warranty .......................................................................................... 28

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 1
Introduction
Thank you for purchasing WPI's UltraMicroPump II (UMP2) and the
microprocessor controller, Micro4™(UMC4). This versatile injector uses
microsyringes to dispense nanoliter sample volumes. Microsyringes are easily
installed by placing the syringe barrel into the UltraMicroPump’s clamps.
UltraMicroPump accepts syringes from 0.5 µL to 250 µL.
UltraMicroPump can be useful for a wide range of applications including
intracellular injection, micro delivery of biochemical agents or dyes, cell
separation, in vitro fertilization, and can be mounted directly onto a stereotaxic
frame or micromanipulator.
Fig. 1 — UltraMicroPump II and Micro4 controller. The pump above
is mounted on WPI’s M3301 micromanipulator and TB-1 stand (not
included); and syringe with luer µTip (not included). At left, pump is
shown with TAXIC900 stereotaxic frame (not included).

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 2
Operating parameters for the UltraMicroPump are set with the Micro4. Up to four
pumps may be independently controlled. Operating parameters set by the user are
stored in “non-volatile” memory for instant recall when the unit is powered on.
An optional footswitch can be plugged into an RS232 port on the rear of the
controller for “hands free” start / stop operation. The same port may also be used to
connect the controller to a computer or to some other device for TTL triggering.
Parts List
After unpacking, make certain that there is no visible damage to the instrument.
Check to see that all items are included:
UMP2 UltraMicroPump II
UMC4 Micro4™Controller, 4-Channel
40300 12-volt Power Supply
Power Cable
Allen key
Instruction Manual
For a list of microsyringes available from WPI, see page 23.
Set-up and Operation
These instructions will help you put the UltraMicroPump to immediate use. We
recommend that you read the entire manual and familiarize yourself with the
various operating procedures of the UltraMicroPump and Micro4.
Mounting the syringe
Syringes may be filled manually before mounting in the UltraMicroPump or filled by
using the fast reverse function (see page 13). Place the plunger button of the filled
syringe into the plunger button holder (leaving the plunger retaining screw loose)
while also placing the syringe collar into the collar stop (see Fig. 2). Take care not
to damage the syringe collar during this installation. Gently tighten the plunger
retaining screw so that the plunger button will not move independently when the
pump is activated (this allows for zero volume error during pump operation).

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 3
Fig. 2 — Initial syringe clamp adjustment — Secure syringe by sequentially
adjusting set screws with supplied Allen key: 1for horizontal alignment, 2for
vertical alignment, and 3to eliminate play.
Axial Needle Alignment
In order to maintain a good syringe needle alignment particularly along the same
axis of the supporting bar, rotate the syringe body while placing in the two clamps
to allow the syringe to seat properly and align along the body of the pump for
minimal slant offset. If the clamp which holds the syringe collar (see “collar stop”
in the drawing below) is too tight or too loose, syringe needle alignment may be off
because of stress.
If the collar stop is too tight to allow the syringe collar to insert easily, then follow
these instructions:
Immediately below and behind the collar stop, in the groove where the long drive
screw is, there is a small Phillips head screw, attached to the collar stop. Loosen
this screw to slightly (about 0.5 to 1 mm) to allow for a thicker collar (see Fig 2).
You may have to grasp the collar stop and wiggle it backwards to move it. Once
the stop is backed out; check and adjust for a tight fit so the syringe body doesn’t
move when placed into the holder. Gently re-tighten the screw in the new position.
Note: you may have to retract the plunger button holder to get access to the set-
screw.
Mount the pump
The UltraMicroPump can
be mounted directly onto
a stereotaxic frame or a
micromanipulator, using
the mounting bar (see
Fig. 2). The mounting bar
may be unscrewed and
modified by the user, if
necessary.
MOUNTING BAR
COLLAR
STOP
SYRINGE
COLLAR
SET SCREW
(not visible in this view)
PLUNGER
RETAINING
SCREW
PLUNGER BUTTON
PLUNGER
BUTTON
HOLDER
CABLE CONNECTS
TO REAR PANEL OF
MICRO4 CONTROLLER
SYRINGE
CLAMPS
CLAMP
RELEASE
SET
SCREWS
SYRINGE
1
2
3

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 4
Footswitch or RS232 cable from
computer plugs into this port.
Connect up to four
UltraMicroPumps.
SWITCH
FOOT
RS232/
+12VDC IN
4
32
POWER ON
1
OFF
OUTPUT
Fig. 3 — Micro4
rear panel
Plug in the 12-volt power
supply.
Mounting to Stereotaxic Frames
WPI’s UMP2 fits directly into most standard stereotaxic frames. The UMP2
mounting bar diameter is 7.90 mm (0.311 in.). For example, UMP2 fits
directly into Kopf Standard 900 series frames (in place of 1770 electrode
holder). Stoelting #51681 V-clamp adaptor / probe holder attachment (WPI
#502201, at left) required for use with Stoelting frames.
Connect the controller
Plug the UltraMicroPump cable into an output socket on the back of the Micro4
controller (see Fig. 3). Up to four pumps may be connected and independently
controlled.
Plug in the 12-volt power supply and power up
Plug the 12-volt power supply into the rear panel of the Micro4 (see Fig. 3). The
switchable power supply included with this unit automatically senses input line
voltage between 100 and 240 V and converts it to 12 V. Connect the power cord
to the power supply and plug it into an electrical outlet. The power switch is also
located on the rear panel of the Micro4.
Switch the Micro4 on and check that the LCD screen is illuminated.
The hardware setup is now complete. Before operating the UltraMicroPump you
must enter the parameters into the Micro4 controller.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 5
Choosing a Syringe
The syringe should be chosen to inject no less than 5% of the volume of any given
syringe.
Example: A 100 µL syringe may be used for injections on the UMPII to volumes of
5 µL (5000 nL) and higher with high precision and repeatability. Expecting this 100
µL syringe to inject lower than 1 µL may be difficult if the syringe is not calibrated
specifically on the pump. The overall accuracy of the syringe itself is usually no
greater than +/-3% and the syringe internal diameter may deviate from location to
location along the length of the syringe interior.
Choosing the correct syringe for an injection is a very important consideration,
due to accumulated errors of the syringe and the pumping method. For a 1000 nL
injection from a 10 µL (10,000 nL) syringe, the user is asking for a 1/10th the
syringe’s volume value to be injected. Theoretically, based on mathematics without
any consideration to surface tension, heat, pressure, compressibility, silanization,
or air bubbles, the 3% error rate should yield a ± 300 nL variance.
As a rule of thumb, the choice of the syringe should not exceed 1/10th to 1/20th the
stated full volume of the syringe. The Micro4-UMP2 system uses a stepper motor
to move the syringe piston forward to inject the volume. A consideration should
also be made to allow the motor to step forward a good number of steps to prevent
errors in volume injecting.
Example: In the 10 µL case above, a 1-step movement of the motor will inject
a volume of 0.5276 nL. For two steps to occur, a volume of 1.055 nL will be
injected. This may or may not be acceptable as the total error may exceed 1
nL or nearly 0.1%. Also note that two steps is probably not enough resolution to
accurately control the volume; another rule of thumb would be to step no less than
10-100 steps for the entire single injection. Using this 10-step rule, the minimum
acceptable injectable volume from this 10 µL (10,000 nL) syringe would be
0.527.6 nL × 10 or 5.276 nL. Other syringes will give other results, due to the inside
diameter and the volume per step.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 6
G = Grouped
N = Non-grouped
D = Disabled
World Precision
Instruments
MicroSyringe Pump Controller
Output 1
Output 2
Output 3
Output 4
VOLUME
SET
(nl)
VOLUME
COUNTER
(nl)
RATE
DEVICE TYPE
123
456
789
.
0
SELECT
DATA
SAVE
RUN
STOP
I=Infuse
W=Withdraw
TM
S=nl/sec
M=nl/min
Arrow keys:
Move the cursor
around the display.
Numeric keypad: Use these keys to
enter settings for infusion and withdrawal
rates and volumes.
Select: Toggles
the selected
parameters: Infuse/
Withdraw, Syringe
Size, Rate Units
(nL/sec or nL/min),
and mode (Grouped,
Non-grouped and
Disabled).
Run/Stop: Starts
and stops the
pump(s).
Data Save: Saves all parameters on LCD display
to memory. Next time the Ultra Microsyringe Pump
controller is turned on, the last paramaters saved will
be displayed.
Lights: When a
pump is running,
the correspond-
ing light is on. For
example, the light
next to output 1 will
be on when pump
one is operating.
Fig. 4 — Micro4
front panel
Setting Parameters
Parameters are entered using the membrane keys on the front panel of the Micro4.
The l e f t and r i g h t a r r o w keys move the cursor on the LCD display to the desired
position. The u p and d o w n a r r o w keys select the channel (corresponding to the
output channels on the rear of the instrument). The n u m b e r keys and the s e l e c t
key are used to change parameters.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 7
Setting Infuse/Withdraw
The character displayed in the first field indicates the operating mode — Ifor
Infuse, Wfor Withdraw. To change modes, use the a r r o w k e y s to position the
cursor in this field, then press s e l e c t .
I = Infuse
W = Withdraw
# = Option
In addition, the options below may be enabled or disabled when the cursor is in
the Infuse/Withdraw field: position the cursor in the Infuse/Withdraw field and press
the corresponding option number.
Disables audible tone.
Enables audible tone.
Changes the action of r u n /s t o p key or footswitch. When this option is
enabled the pump will operate as long as the r u n /s t o p key or footswitch is
pressed and stop when the r u n /s t o p key or footswitch is released.
Returns r u n /s t o p key or footswitch to normal operation.
Sets Volume per Step for syringe types not already preset in the Micro4’s
memory (see page 14).

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 8
Volume Set
To select the desired volume to be infused or withdrawn, position the cursor in
the volume set field and enter the numbers with the numeric keypad. The values
shown on the LCD display are in nanoliters. (Note: For 10 µL enter “10000”; for
1.0 µL enter “1000.”—including decimal point.)
Syringe Injection Accuracy: Since every syringe in the microliter volume range
has its own unique intricacies, you should verify and calibrate each syringe and log
its characteristics for accurate injections. The Micro4 controller has preset types
of syringes to very accurately move the plunger button of the syringe a precise
distance per injection.
VOLUME
SET
(nL)
Volume Counter
Real-time display of volume being delivered. When pump is not running, this
number may be changed; when pump is restarted, counter will continue from the
number entered.
VOLUME
COUNTER
(nL)

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 9
Injections beyond 99,999 nL (5 digits in the volume field)
Example: You want to inject 150,000 nL from a 500 µL liquid tight syringe, but the
volume counter can not accept 150,000 as a number.
1. Take the value 150000 / 500000 = 0.3 or 30% of the syringe volume.
2. Use a syringe TYPE that can accommodate a proportionally reduced number
of the same injection percentage (in this case 50%).
3. Using TYPE H, a 250 µL syringe as the choice a 75,000 nL injection is
equivalent to a 150,000 nL injection in a 500 µL syringe.
4. Multiply times 60 mm for the actual distance travel 0.3 × 60 = 18 mm (60 mm is
the full volume stroke of the syringe).
5. Check: 75,000 / 250,000 = 0.3; 0.3 × 60 mm = 18 mm.
6. Any combination of syringe types and volume choices can be used to displace
a particular length, as long as the volume is noted and the injection speed
does not exceed the rated value nearest that syringe volume. See syringe
tables, page 11.
Rate setting / unit time
To select the rate for infusion or withdrawal, position the cursor in this field and type
in the desired value with the numeric keypad. If the rate entered is too large for
the selected syringe type, the highest possible value will be displayed in this field.
Next, select the rate units.
RATE

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 10
Rate Units
Two rate units are available — nanoliters per second and nanoliters per minute.
Position the cursor in this field and use the s e l e c t key to choose either S(nL/sec)
or M(nL/min).
S = nl/sec
M = nl/min
DEVICE TYPE
Device (syringe) Type
The volume per step and rate data for eleven microsyringes are already stored in
Micro4’s memory. To specify one of these syringes, position the cursor in this field
and use the s e l e c t key to change the syringe type to a letter (A- L) correspond-
ing to that of the syringe in the table below.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 11
Type Syringe Volume
(Defined for this length) ID (mm) nL / step REV J
Max. Rate
nL /sec or
nL /min
A0.5 µL (54.1 mm)
† 0.1085 (0.102 REV H) 0.0294 ( 0.026 Rev H) 20
B1.0 µL (54.1 mm)
† 0.1534 (0.145 REVH) 0.0587 (0.052Rev H) 40
C** 5 µL (54.1 mm)
† 0.343 (0.325 REV H) 0.2934 (0.263 Rev H) 202
D10 µL (54.1 mm)
† 0.485 (0.46 REV H) 0.5868 (0.528 Rev H) 406
E25 µL (60.0 mm) 0.73 1.329 1022
F50 µL (60.0 mm) 1.03 2.646 2035
G100 µL (60.0 mm) 1.46 5.315 4088
H250 µL* (60.0 mm) 2.3 13.191 9999
I500 µL* (60.0 mm) 3.26 26.501 9999
J1000 µL* (60.0 mm) 4.61 52.995 9999
KNanoliter 2000
† † 0.48 plunger in 0.50 glass 2.3 nL /step (0.0005” step) 884
L10 µL (60 mm) 0.4607 (0.100 Rev H) 0.5293 (0.0249 Rev H) 170
MUser Defined See page 12
** ILS005 (27 mm) 0.4856 0.5880 compensates for length as
TYPE M
* Gas-tight syringes are not recommended for UMPII in these volumes; instead, use a liquid-tight
syringe to prevent drive motor damage.
** The ILS005 5 µL syringe must use type M with a 0.5880 nL / step entry. See page 15 for details.
† These are defined as 60 mm in Rev. H firmware.
††WPI’s Nanoliter 2000, a nanoliter injector for the 2-70 nL range, comes with its own simple controller
but may also be driven by the Micro4. For more information, enquire about WPI # B203XVY.
Note On Syringe Stroke Length
The delivery of the UMPII is based on 60 mm or 54.1 mm syringes. Please note
which syringe length you are using as a factor of 0.9016 may need to be applied to
the volume to be injected in or-
der to have a precise injection.
For the ILS005, use the TYPE
“M” values in the above table.
Maker Syringe Stroke
Length Use TYPE
Hamilton 1700 Series ,10 µL 60 mm L
Hamilton 700 Series,10 µL 54.1 mm D
SGE 0.5 µL – 10 µL 54.1 mm A,B,C,D
ILS 5 µL Luer tip ILS005 28 mm M
SGE 25 µL – 500 µL 60 mm E - L
WPI FlexiFil™ 54.1 mm D
WPI NanoFil™ 60 mm L
*Manufacturers have the right to
change this specification at any time.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 12
G = Grouped
N = Non-grouped
D = Disabled
(blank)
Grouped/Non-grouped/Disabled
For convenience in operating multiple pumps (whether of identical or various vol-
umes), pumps may be grouped or non-grouped. Pressing the s e l e c t key while the
cursor is in this field will toggle through three operating modes:
Grouped mode: Syringe channels with “G” in this field are started or
stopped when the r u n /s t o p key is pressed while the cursor is located on any
grouped channel.
Non-grouped mode: When the cursor is positioned on a channel that is
not grouped, indicated by the letter “N”, only that channel will start or stop
when the r u n /s t o p key is pressed.
Disabled mode: When a channel is disabled, the line of data is hidden and
the pump will not operate. No changes may be made to this channel while
it is disabled. To re-enable it, move the cursor back to the Group/Nongroup
field and press the s e l e c t key; the previously entered data will be restored.
Saving your settings
Pressing the button will store these values into the controller’s memory for
future use.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 13
UltraMicroPump II Operation
When the pump runs, a series of beeps indicates that the pump is running. At
the end of the program, the controller also beeps. A lamp on the back of the
UltraMicroPump indicates a signal from the controller. As the pump runs, the
counter increments as an indication of the plunger’s motion. Multiple injections can
be achieved by pressing the r u n /s t o p button again after the pump has stopped.
Fast forward
Press and hold the r i g h t a r r o w key then press r u n /s t o p . The syringe
pump will continue running as long as these two keys are depressed or until the
set volume is reached.
Fast reverse
Press and hold the l e f t a r r o w key then press r u n /s t o p . The syringe
pump will continue running as long as these two keys are depressed or until the
set volume is reached..

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 14
Defining Other Syringes
Eleven microsyringes with volumes ranging from 0.5 µL to 1000 µL are already
preset in the Micro4 (syringe types Athrough L— see table on page 11). A mi-
crosyringe with a volume other than those preset may be entered as device type
M. However, the Volume per Step of the type Msyringe must be defined for the
Micro4. NOTE: There is only one memory location to define a Type M value for all
four pumps.
First, calculate the Volume per Step using the formula below. Syringe displace-
ment is the distance between 0 and the maximum volume marked on the syringe in
inches. Syringe volume is in nanoliters. ID is in millimeters.
Syringe of known ID
Volume per Step = (ID/2) x (ID/2) x 3.1415926 x 1000 x 0.003175
Syringe of unknown ID
The Inside Diameter (ID) of a syringe can be determined by this formula:
ID = SQRT(Volume/(3.14159 x Length)) x 2
where length is the visible marking on the syringe body (total length in mm) and
Volume is the full volume of the syringe in microliters.
For example, if you have a 60 mm syringe with a
2.5 µL volume, use the formula above to determine
its Volume per Step. Move the cursor to the Volume
Set field of output channel 1 and enter the calculated
value (in this case, 0.1323 nL):
Then use the l e f t a r r o w key to scroll the cursor to the
first position on the LCD display and press . This sets your calculated definition
for your type Msyringe into the Micro4’s memory.
Calculate in Microsoft
Excel — just enter
these formulae into
a spreadsheet,
replacing Volume,
Length, and ID
from your own
measurements.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 15
Note that this does not yet affect the action of any channel since Mhas not been
selected as the device type for any channel. Before proceeding, move the cur-
sor back to the Volume Set field and re-enter the correct volume for the syringe on
output channel 1 (which may be any type — not necessarily type M).
Special Syringe Considerations ILS005
The UMPII-Micro4 was designed around 60 mm or 54.1mm syringes. The full
injection of a 5 µL syringe is expected to be a 60 mm movement. In the case of this
ILS special 5 µL glass luer tip syringe this length is 27 mm for an injection of 5 µL.
There are two solutions:
Solution 1. Using Type “C” (the 54.1 mm 5 µL syringe), Multiply the volume
to be injected by 27/54.1 or (0.4990).
Example. A 2500 nL injection using type C should be entered into the micro4
as 2500 × 0.4990 or 1247.5 nL. this value can be further calibrated see page
17 for details.
Solution 2. Using Type M, divide the Type C nL/step number by 0.4990.
Example: The standard 54.1 mm syringe has a value of 0.2934 nL/step on
a 5 µL syringe. The ILS005 has a 27mm stroke for this same full volume.
0.2934 / 0.4990 = 0.5880 is the nL / step value to enter following the Type
M instructions on this page. (0.5880 is also verified by the formulas on the
previous page.)
After defining your syringe in Micro4’s memory, move the cursor to the Device Type
field of the output channel to which that pump is connected and press s e l e c t until
Mappears.
Any number of channels may use type Msyringes, but
since a single definition for type Mis stored in Micro4 all
Mdevices must be identical. (That is, you cannot use
two non-standard types, such as 2.5 µL and 0.25 µL.)
The minimum delivered volume depends on the syringe
size and is listed in the syringe type table under Volume
per Step. The actual volume delivered is divisible by the volume per step. For
example, using a syringe with a Volume per Step of 1 nL, actual delivered volume
for the given set volume is listed below.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 16
Volume Set Actual volume delivered
0-0.9999 nL 0
1 nL-1.999 nL 1 nL
2 nL-2.999 nL 2 nL
and so on...
Using Teflon Tipped syringes
Carefully remove the plunger and its Teflon tip by drawing it out of the syringe
barrel.
1. Before inserting the plunger tip into the syringe, pre-wet the Teflon plunger tip
and the syringe body interior with water.
2. Use care in inserting the plunger into the syringe as the plunger rod may be
easily bent.
3. Carefully place the plunger tip into the syringe and gently work the tip down
into the body of the syringe using a thumb and forefinger to grasp and push
small lengths of the plunger rod into the syringe. Repeat this procedure until the
plunger tip is near the zero mark of the syringe.
4. Draw additional water into the syringe and slowly work the plunger up and down
until the plunger tip is cold formed into the syringe and the stiffness goes away.
The stiffness of the new plunger tip may require you to move the rod in small
increments until the tip is formed enough to actuate by the rods full length.
Wet Autoclaving Syringes
Typically the answer to autoclaving any Teflon syringe is no, since the adhesives
and the Teflon seal will eventually breakdown or swell from the heat and pressures
involved. The most practical method of sterilizing is either gas or liquid chemical
sterilization, but both require meticulous removal of the sterilizing agents prior to use.
If you are willing to replace the syringe after a few uses then most syringes will
stand up to a few cycles of wet autoclaving. The Teflon tipped plunger should be
removed for this operation. Careful examination of any glued components and the
Teflon tip integrity is required before reuse of an autoclaved syringe. If the Teflon tip
cannot be replaced into the syringe body easily then the plunger (tip) and perhaps
the syringe requires replacement due to infusion of water. Autoclaving will usually
void the warranty on Teflon tipped syringes.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 17
Calibration
Every syringe should be calibrated on the pump that it is being used with.
This gives the user two things, verification of the error involved in the injection and
the confidence that injection is correct. Micro volume syringes are rated at 1% to
3% of the full-scale volume. So for a 10 µL syringe injecting 10 µL there will be a
maximum error of ± 0.3 µL if the injection takes place along the markings on the
syringe barrel. When used in a specialized syringe pump like the UMPII, this same
syringe is now defined by a fixed length and moved by a precision stepper motor.
This can offer a very high degree of precision and repeatability. This same 3% error
10 µL syringe can now be calibrated to deliver a reduced error of ± 0.5% tolerance
or better.
Two methods of calibration
Volumetric diameter measurement
Using a microscope and a calibrated reticle or stage micrometer, inject an
amount of water into a hydrated oil droplet, measure the sphere and use
Volume = ((Diam/2) × (Diam/2) × (Diam/2) × 3.1415926 × (4/3) × 1000))
to determine the volume in nanoliters. (Diameter in mm.)
Analytical Balance
Use an analytical balance to weigh the mass of the injected volume. (Pure
water is 1 gm = 1 mL at 4 degrees C.) Tables for other temperatures are
easily obtained.
Calibration on the Pump
Method 1: Multiply the ratio of the injected volume by the actual volume and
adjust the volume injected accordingly.
Method 2 : Use the TYPE M syringe and enter the new nl/sec number after
recalculation.
Note: It may be necessary with some syringes to verify injections at different
locations along the length of the syringe barrel as there can be variations along the
inside length of the glass barrel.
Calculate in Microsoft
Excel — just enter
these formulae into
a spreadsheet,
replacing Volume,
Length, and ID
from your own
measurements.

UltraMicroPump II
WORLD PRECISION INSTRUMENTS 18
Computer Control
RS232 commands are used to control the Micro4 via the serial port of a PC or
Macintosh computer.
RS232 Commands
All commands are case sensitive. The settings for the RS232 port are baud rate
9600, 8 data bits, 1 start bit, 1 stop bit. Microsoft HyperTerminal setting: Flow con-
trol must be set to NONE.
Numbers and decimal points are indicated below by the “#” symbol.
V######; Sets the delivered volume. Number must have a decimal point and
terminate with “;” (semicolon) — see page 19.
C######; Sets the volume counter. Number must have a decimal point and
terminate with “;” (semicolon) — see page 19.
R####; Sets the delivery rate. Number must have a decimal point and
terminate with “;” (semicolon) — see page 19.
IInfuse mode.
W Withdraw mode.
GGo — Starts the syringe pump.
HHalt — Stops the syringe pump.
SSets the rate units to nanoliters/second.
MSets the rate units to nanoliters/minute.
L#; Line number — sets the syringe number on display (Micro4 only).
NNot Grouped mode.
PGrouped mode.
DDisabled mode.
TxSyringe Type. The letter indicating syringe type follows the T. For
example, to select syringe type “A” the command is “TA”.
RS232 Query Commands
All query commands begin with a question mark. Below is a list of the query
commands.
?V Returns the set volume.
?C Returns the volume counter
?R Returns the delivery rate