wtw OxiTop C User manual

ba31114e06 06/2012
OxiTop®
Operating manual
OC 100
®
ON/OFF
GLP
RUN/ENTER
®
-C
Controller
OxiTop®OC 100
Measuring head
OxiTop®C

OxiTop®Control OC 100
2 ba31114e06 06/2012
Copyright ©Weilheim 2012, WTW GmbH
Reproduction in whole - or even in part - is prohibited without the express
written permission of WTW GmbH, Weilheim.
Printed in Germany.

OxiTop®Control OC 100 - Contents
OxiTop®Control OC 100 Contents
ba31114e06 06/2012 3
1 Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 The system OxiTop®Control . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 The measurement principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Data management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.4 Literature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Safety information in the operating manual . . . . . . . . . . . . 9
2.1.2 Safety signs on the instrument . . . . . . . . . . . . . . . . . . . . . 9
2.2 Safe operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.1 Authorized use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.2 Requirements for safe operation . . . . . . . . . . . . . . . . . . . 10
2.2.3 Unauthorized use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2.1 Batteries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2.2 Battery status signals. . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2.3 Energy saving feature (automatic switch-off). . . . . . . . . . 12
3.3 Initial commissioning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3.1 Inserting the supply battery holder - controller. . . . . . . . . 12
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 General operating principles . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1.1 Keys - controller. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1.2 Socket field - controller . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.2 Switching on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.2.1 Switching on the controller. . . . . . . . . . . . . . . . . . . . . . . . 16
4.2.2 Switching on the measuring heads . . . . . . . . . . . . . . . . . 16
4.3 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3.2 Menus, functions and dialogs . . . . . . . . . . . . . . . . . . . . . 17
4.3.3 The course of the measurement . . . . . . . . . . . . . . . . . . . 18
5 Operating mode: BOD Standard / BOD Routine . . . . . . . . . 19
5.1 Sample preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2 Starting the measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.3 Calling up all data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.4 SAMPLE STATUS TYPE list (sample management) . . . . . . . . 24
5.4.1 Showing a sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Contents OxiTop®Control OC 100
4ba31114e06 06/2012
5.4.2 Erasing data of finished samples . . . . . . . . . . . . . . . . . . 26
5.4.3 Showing the measuring head list . . . . . . . . . . . . . . . . . . 27
5.4.4 Calling up data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.4.5 Calling up data - Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.5 Evaluation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.5.1 Sample statistics (BOD Standard operating mode) . . . . 31
5.5.2 Excluding a curve (BOD Standard operating mode) . . . 32
5.5.3 Cursor interrogation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5.4 Curves display for cold samples. . . . . . . . . . . . . . . . . . . 34
5.5.5 Measured values outside the measuring range . . . . . . . 35
6 Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7GLP/TOOLS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
7.1 GLP/TOOLS main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
7.2 Show free measuring heads . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.3 Show settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.4 Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7.4.1 Operating mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7.4.2 Operational life . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.4.3 Date/time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.4.4 GLP (BOD Standard operating mode). . . . . . . . . . . . . . 42
7.4.5 GLP - calibration interval (- Calinterval) . . . . . . . . . . . . . 43
7.4.6 Data storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7.4.7 AutoTemp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
7.4.8 Switch-off interval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.4.9 Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.5 Check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.5.1 Showing the measuring heads . . . . . . . . . . . . . . . . . . . . 47
7.5.2 Measuring head information . . . . . . . . . . . . . . . . . . . . . . 48
7.5.3 Controller information . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.5.4 cal-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.5.5 Pneumatic test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.6.1 Erasing finished samples . . . . . . . . . . . . . . . . . . . . . . . . 54
7.6.2 Reset/release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
7.6.3 Restoring data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
8.1 Achat OC software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
9 Maintenance, cleaning, disposal. . . . . . . . . . . . . . . . . . . . . . 61
9.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
9.1.1 General maintenance activities . . . . . . . . . . . . . . . . . . . 61
9.1.2 Replacing the supply batteries (controller) . . . . . . . . . . . 61
9.1.3 Replacing the data backup battery (controller). . . . . . . . 63
9.1.4 Changing the supply battery (measuring head) . . . . . . . 65
9.2 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
9.2.1 Cleaning of the sample bottles . . . . . . . . . . . . . . . . . . . . 66
9.2.2 Cleaning of the controller and measuring heads . . . . . . 66
9.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

OxiTop®Control OC 100 Contents
ba31114e06 06/2012 5
10 What to do if.... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
10.1 Display messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
10.2 Display messages on the charging status of the batteries . . . 72
10.2.1 Batteries in the controller . . . . . . . . . . . . . . . . . . . . . . . . 72
10.2.2 Battery in the measuring head . . . . . . . . . . . . . . . . . . . . 72
10.3 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
11 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.1 Controller OxiTop®OC 100 . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.2 OxiTop®-C measuring head. . . . . . . . . . . . . . . . . . . . . . . . . . . 78
12 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Contents OxiTop®Control OC 100
6ba31114e06 06/2012

OxiTop®Control OC 100 Overview
ba31114e06 06/2012 7
1 Overview
1.1 The system OxiTop®Control
The classical application field for the OxiTop®measuring heads and controller
is the BODx determination (BODx = Biochemical Oxygen Demand for the time
x). The evaluation of biological degradability (e.g. test according to OECD
301F) is also part of this field.
With different sample vessels, the OxiTop®-C measuring heads in conjunction
with the OxiTop®Controller 110 can also be used in other areas such as
Evaluation of respiration and toxicity in earth, sludge, waste and sediment
(e.g. extraction of contaminated earth according to recovery concepts)
Evaluation of the respiration rate of cell cultures
Microbiological growth and stress examinations
Measurement of anaerobic degradation processes (e.g. biogas determina-
tion)
Accessories: see WTW catalog and price list
1.2 The measurement principle
The respirometric measurement is a pressure measurement. If oxygen is
consumed in a closed vessel at a constant temperature, a negative pressure
develops. If a gas is released, an overpressure develops. The OxiTop®-C
measuring head measures and stores this pressure for the whole duration of a
measurement once started.
The OxiTop®OC 100 controller collects the pressure values from the
measuring heads and processes them.
The formula shown below is the basis for all calculations for the BOD using the
values from the OxiTop®-C measuring head.
The interpretation of the pressure differences in the temporal course depends
on the measured material and its preparation and on the sample manipulation
(e. g. intermediate aerations) during the measuring period.
1.3 Data management
The measuring head records the measured values and stores the measuring
data. Using the controller, the collected and stored data are read out from the
measuring head and stored in the “sample management“. Starting at the
OxiTop®sample management, it is possible to make the graphical and statis-
tical evaluation of the sample data.
The data can be transferred from the controller to a computer via the RS232
interface. The read-out software Achat OC facilitates to process the stored data
further.

Overview OxiTop®Control OC 100
8ba31114e06 06/2012
Additionally, the data can be transferred to an IR printer via the IR interface.
1.4 Literature
Further information and application reports on the subject of measuring with the
system OxiTop®Control can be obtained at no cost from WTW:
CD-ROM applications for OxiTop®Control
CD-ROM Principles of measuring technique

OxiTop®Control OC 100 Safety
ba31114e06 06/2012 9
2Safety
2.1 Safety information
2.1.1 Safety information in the operating manual
This operating manual provides important information on the safe operation of
the instrument. Read this operating manual thoroughly and make yourself
familiar with the instrument before putting it into operation or working with it.
The operating manual must be kept in the vicinity of the instrument so you can
always find the information you need.
Important safety instructions are highlighted in this operating manual. They are
indicated by the warning symbol (triangle) in the left column. The signal word
(e.g. "CAUTION") indicates the danger level:
NOTE
indicates a possibly dangerous situation where goods might be damaged
if the actions mentioned are not taken.
2.1.2 Safety signs on the instrument
Note all labels, information signs and safety symbols on the instrument. A
warning symbol (triangle) without text refers to safety information in this oper-
ating manual.
2.2 Safe operation
2.2.1 Authorized use
Only the operation and running of
the instrument according to the instructions and technical specifications given
in this operating manual is authorized (see section 11 TECHNICAL DATA, page
77).
Any other use is considered unauthorized.
WARNING
indicates a possibly dangerous situation that can lead to seri-
ous (irreversible) injury or death if the safety instruction is not
followed.
CAUTION
indicates a possibly dangerous situation that can lead to slight
(reversible) injury if the safety instruction is not followed.

Safety OxiTop®Control OC 100
10 ba31114e06 06/2012
2.2.2 Requirements for safe operation
Note the following points for safe operation:
• The instrument may only be operated according to the authorized use spec-
ified above.
• The instrument may only be supplied with power by the energy sources
mentioned in this operating manual.
• The instrument may only be operated under the environmental conditions
mentioned in this operating manual.
• The instrument may only be opened if this is explicitly described in this oper-
ating manual (example: Changing the batteries).
2.2.3 Unauthorized use
The instrument must not be put into operation if:
• it is visibly damaged (e.g. after being transported)
• it was stored under adverse conditions for a lengthy period of time (storing
conditions, see section 11 TECHNICAL DATA, page 77).

OxiTop®Control OC 100 Commissioning
ba31114e06 06/2012 11
3 Commissioning
3.1 Scope of delivery
Controller OxiTop®OC 100
Battery holder with batteries (3 x Mignon, AA, AM3, LR6)
Operating manual
Additional scope of delivery: depending on set composition
3.2 Power supply
The controller OxiTop®OC 100 and measuring head OxiTop®-C are both
battery-powered.
Additionally, the controller has a data backup battery. This battery ensures that
your measuring data and instrument settings are stored in the controller
OxiTop®OC 100.
3.2.1 Batteries
3.2.2 Battery status signals
The OxiTop®-OC 100 controller and the OxiTop®-C measuring heads are pow-
ered by batteries. A low battery status of the controller or measuring head is
automatically indicated by a display message and a signal tone after the instru-
ment is switched on (see section 10.2 DISPLAY MESSAGES ON THE CHARGING
STATUS OF THE BATTERIES, page 72).
The current battery status can also be queried in the menu:
Controller: Menu GLP/TOOLS -Check- Controller Info
Measuring head: Menu GLP/TOOLS - Check -info
Controller Power supply 3 x alkaline batteries (alkali manganese),
Size: Mignon, AA, AM3, LR6
Runtime: > 100 h
(approx. 1000 start-ups in normal use)
Data backup 1 x lithium battery CR2430 (accessory).
Runtime: typically 4 years
Measuring
head
Power supply Batteries: 2 x lithium battery CR2430 (accessory).
Runtime: typically 2 years
1st level (warning level) Battery LoBat !
2nd level (error level Battery empty !

Commissioning OxiTop®Control OC 100
12 ba31114e06 06/2012
3.2.3 Energy saving feature (automatic switch-off)
The instrument switches off automatically following the last key actuation after
expiry of the set switch-off interval.
To set the switch-off interval: see section 7.4.8 SWITCH-OFF INTERVAL, page 46.
3.3 Initial commissioning
Perform the following activities:
Insert the supply battery holder in the controller
(see section 3.3.1 INSERTING THE SUPPLY BATTERY HOLDER - CONTROLLER,
page 12)
Switch on the instrument
Set the date and time (see section 7.4 SETTINGS, page 40)
3.3.1 Inserting the supply battery holder - controller
Loosen the 4 screws underneath the housing using a Phillips screwdriver.
Place the controller on the lower case.
Remove the upper case and put it down to the right next to the lower case
with the display downwards.
Connect the supply battery holder with the power supply contacts of the
upper case.
Insert the supply battery holder in the lower case with the open side down.
1Screws
LISTED 8F93
Laboratory Equipment
E163694
This device complies with Part 15 of the FCC Rules. Operation
is subject to the following conditions: (1) This devise may not
cause harmful interference, and (2) this devise must accept
any interference received, including interference that may
cause undesired operation.
Ser.-Nr.:05150013
Oxi Top OC 1x0
WTW 82362 Weilheim
Made in Germany
1x CR 2430 Lithium
3x Alkaline, AA size, 1,5V
11
11

OxiTop®Control OC 100 Commissioning
ba31114e06 06/2012 13
Set the upper case on top of the lower case, turn the controller and tighten
the housing screws using the screwdriver.
Switch on the controller. The battery insertion was successful if no error
message appears concerning the supply batteries.
1 Supply batteries in the battery holder (lower case)
2 Data backup battery (upper case)
1
2

Commissioning OxiTop®Control OC 100
14 ba31114e06 06/2012

OxiTop®Control OC 100 Operation
ba31114e06 06/2012 15
4 Operation
4.1 General operating principles
4.1.1 Keys - controller
ON/OFF
GLP
RUN/ENTER
1
2
5
34
7
8
6
The function keys are used to start a function or to change to another
function. Confirmed data and settings are preserved.
1<On/Off>Switching on/off
2 <PRINT> Printing of measurement data and set-
tings via IR interface
3 <RUN/
ENTER>
Confirmation of entries
4<><> Select and change settings
Function keys:
5 <ORG> Sample management: List of samples,
reading/calling up/deleting the data of indi-
vidual measuring heads or samples,
shows measuring heads or samples
6 <COMM> Communication with measuring heads:
Start measuring, change or show settings,
call up data
7 <GRAPH> Evaluation: Graphic and numerical dis-
play of measuring data
8 <GLP> GLP/Tools: Change or display settings,
display free measuring heads, display or
change settings, perform checks or main-
tenance
ON/OFF
RUN/ENTER
GLP

Operation OxiTop®Control OC 100
16 ba31114e06 06/2012
4.1.2 Socket field - controller
4.2 Switching on
4.2.1 Switching on the controller
4.2.2 Switching on the measuring heads
The measuring heads are immediately ready for operation. The controller
switches the measuring heads on and off during communication.
4.3 Navigation
4.3.1 Operating modes
The current date and time appear for approx. 2
seconds (important for the allocation of sample
numbers).
If the date or time is wrong, correct them under
GLP/TOOLS.
The instrument is in the sample management
(BSB-Routine BOD Standard mode, in the deliv-
ery state).
To avoid malfunctions:
If you use two or more controllers simultaneously make sure that
the distance between the controllers is at least 3 meters!
BOD Routine:
(preselected)
Measurement of single samples for a BODx(x = 0.5h ... 99d) at 20 °C
The measuring range and filling volume can be selected out of seven per-
manent ranges and the corresponding filling volumes.
The function is permanently switched on
BOD Standard:Measurement of up to 12 parallel sample processes per overall sample for
a BODx(x = 0.5h ... 99d) at 20 °C
The measuring range and filling volume can be selected out of seven per-
manent ranges and the corresponding filling volumes.
Automatic sample statistics with averaging
The AutoTemp function and the GLP mode can be switched on

OxiTop®Control OC 100 Operation
ba31114e06 06/2012 17
4.3.2 Menus, functions and dialogs
Example: Pressing the <COMM> button causes the controller to change to the
SELECT ACTION menu (communication with the measuring heads).
Signal tone
Measuring head Each measuring head that receives a command from the controller indicates
this by a short flashing signal.
The OxiTop®-C measuring head is indicated with the symbol
Action:
Press button. The instrument displays:
Description of what the
action caused and possible
further request:
SELECT ACTION menu
(communication with the mea-
suring heads):
Preselected: -Startsample.
Select a menu item with
<><>.
The selected function or line is
displayed in inverse video.
Confirm the menu item with
<RUN/ENTER>.
The submenu is opened or the
function is carried out.
This symbol in the operating manual means:
A signal tone sounds.

Operation OxiTop®Control OC 100
18 ba31114e06 06/2012
4.3.3 The course of the measurement
Preparation before
starting the mea-
surement
Measuring
Select operating mode
Select settings
Operating mode and settings cannot be changed for a measure-
ment once it is
started!
GLP
Time
End of measurement
Start of
measurement
AutoTemp Measurement
Data transfer
Starting the
measuring
heads
Sample
management
Evaluation
Print
Print

OxiTop®Control OC 100 Operating mode: BOD Standard / BOD Routine
ba31114e06 06/2012 19
5 Operating mode: BOD Standard / BOD Rou-
tine
The BOD Standard mode (parallel sample process for up to 12 measuring
heads per overall sample) is preset in the delivery condition of the controller.
To change to the BOD Routine operating mode (single samples): see section
7.4.1 OPERATING MODE, page 40.
The handling of controller and measuring heads is basically the same in both
operating modes.Where there are differences for the BOD Routine operating
mode they are described.
5.1 Sample preparation
See application reports.
Screw the OxiTop®-C measuring heads onto the BOD bottles and
close them tightly.
NOTE
Never use joint grease or other lubricant for your OxiTop®-C measuring
heads. Some of these products contain solvents that can cause severe
damage to the plastic housing of the measuring head. The sealing of the
BOD bottles is also perfectly adequate without grease. However, you
should always wipe off heavy contamination and particles on the sealing
surfaces of the rubber sleeves and OxiTop®-C. WTW accepts no liability
for damage due to the use of joint grease.
5.2 Starting the measurement
SELECT ACTION menu (commu-
nication with the measuring
heads):
Preselected: - Start sample.
(In BOD Routine mode appears:
START).
Select the measuring range with
<><>.
The filling volume required is
given in the right hand column.
The controller stores the setting
(memory function: the last
selected measurement range is
set).
RUN/ENTER

Operating mode: BOD Standard / BOD Routine OxiTop®Control OC 100
20 ba31114e06 06/2012
Confirm the selected
measurement range for the
sample. The automatically
assigned sample number
(YY/MM/DD and sequential
number) is given in the header
line.
Additional information:
Type of measurement, run duration, measurement range,
final date, ID number.
Temp display => the AutoTemp function (see section 7.1
GLP/TOOLS MAIN MENU, page 38) is switched on.
GLP display => the GLP function (see section 7
GLP/TOOLS, page 38) is switched on.
Change the ID number for the additional identification of the
sample (e.g. sampling location) as follows:
Select the - I.D. number menu item with <><>
Confirm with <RUN/ENTER>.
Set the required ID number (setting range 001 ... 255) with
<><>.
Confirm with <RUN/ENTER>.
Use <PRINT> to print out the entire sample information (in BOD
Routine mode only), see section 6 PRINTING, page 36).
Confirm the start of
measurement. Contact selection:
The controller repeatedly sends the start information in the
scanner mode until successful feedback is received from the
OxiTop®-C measuring head. After the successful start message
from the measuring head:
Displays ! started !
From this point in time, the sample
exists within the
SAMPLE STATUS TYPE list
(sample management).
Subsequently, the request to
start the next measuring head is
made automatically.
RUN/ENTER
RUN/ENTER