Yamato PiCOEXPLORER PAS-110-YU User manual

Yamato Scientific America, Inc.
User's Manual and Troubleshooting Guide
PA S -110 -Y U
Supported Version
Android: 3.1.1
iOS: 2.0.8
Photo Absorbance Sensor
Contents of this User’s Manual may change without notice.
Due to design improvements, and/or specication and app changes,
PiCOEXPLORERTM conguration may change without notice.
All company names and products names in this manual are the property of their
respective trademark holders.
Patent Pending

SAFETY NOTES
DEVICE FEATURES HARDWARE PACKAGE
HARDWARE COMPONENTS
APP INSTALL BATTERY INSERTION
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TROUBLE- SHOOTING
WARRANTY AND AFTER-SERVICE
SPECIFICATIONS
LAWS AND
REGULATIONS
Thank you for purchasing the PiCOEXPLORERTM PAS-110-YU photo absorbance sensor.
This manual contains detailed descriptions, instructions for use, and specifications of this device.
Please print out and store this manual in a safe place, and refer to as needed.
These safety notes are intended to help you use this device properly and safely. These notes contain
essential and crucial instructions that must be followed at all times. Please read and understand these notes
before reading the rest of the manual and guide.
SAFETY NOTES
WARNING
●
Do not use the device near medical equipment. The radio waves emitted by wireless communica-
tion module could have an adverse effect on nearby pacemakers or electrical medical devices.
●
Do not use near automatic doors, alarms, or other automatic electrical control devices.
The wireless communication module could cause malfunctioning in these devices.
●
Do not use any USB cables that have been damaged or deformed. Doing so could result in smoke,
overheating, or fire.
● Carefully read and correctly follow the instruction in this user’s manual about the use of USB ca-
bles. Replace any cable that looks defective or damaged.
● Do not wash the device with water, or disassemble or tamper with it in any way.
Doing so could result in electric shock, fire, or smoke.
●
When powering the device thru a USB connector, use a PC with a limited-energy circuit USB out-
put, or an AC adapter with a limited-energy circuit used to power PCs.
CAUTION
HANDLING OF PHOTO ABSORBANCE SENSOR
●This device is for general analytic use. It was not designed for, nor should it be used for, medical
diagnostic purposes requiring very high levels of reliability and/or accuracy.
Yamato Scientific Co., Ltd. cannot be held responsible for any damages or liabilities resulting from
the inappropriate use of this device for such purposes.
●To avoid eye injury, never look directly at the light source in the device.
●When connecting and removing the USB cable, do so carefully; do not jam the connector or yank
the cord.
●Avoid dropping the unit or otherwise exposing to shocks or extreme forces.
HANDLING OF BATTERIES
●Follow all instructions of this manual on the handling and use of dry-cell batteries.
●Use only the battery type specified (AAA type). Other types may damage the unit.
●Insert batteries properly, matching the plus and minus ends to the equivalent connections inside
the unit.
●When replacing batteries, always replace all 3 batteries together.
●Keep batteries out of the hands of small children.
●If the unit will not be used for some time, remove the batteries before storage. Leaking batteries
can cause severe damage.
WARNING
CAUTION
This icon indicates a possible danger of loss of life or injury.
This icon indicates a possible danger of injury to personnel or physical damage.
This icon indicates important information concerning easy-to-make mistakes that can adversely
affect operation and maintenance. Not following the instructions given could result in damage to
the device.
IMPORTANT
SAFETY NOTES
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SAFETY NOTES
HANDLING OF BATTERIES
●Do not expose batteries to heat or toss into open flames.
●For the sake of the environment, dispose of used batteries responsibly and in accordance with
local regulations.
●The batteries that come packaged with the unit were inserted only for device testing purposes.
They may not last long; replace them as soon as possible.
HANDLING OF A BLUETOOTH DEVICE
●This is a Bluetooth low-energy compatible device. It cannot be connected with any other wireless
communication protocol.
●This device has been certified as having the proper wireless system for low-power electronic
communication. Do not remove the label of certification from the device. It is against the law to
tamper with the device by disassembling, and/or altering operation. Doing so may result in
criminal prosecution.
●This device communicates using a 2.4GHz frequency range. This range is also used by the
following wireless transmitters: commercial, scientific, and medical devices and other similar
devices, wireless transmitters used to detect moving object on factory lines (which require
licensing), low-power wireless transmitters which do not require licensing, and amateur wireless
transmitters, all of the above designated herein as “other wireless transmitters”. Before using,
make sure there is no such wireless transmitter in the vicinity which could cause interference. If
you find that the operation of this device near another wireless transmitter is causing
interference, increase the distance between the devices or if possible, turn off the other device.
●Do not use in areas with high levels of dust, oil smoke, steam, damp, or high heat.
●If the unit gets wet or submerged in liquid, immediately turn off and consult your vendor.
●Dirt and other contaminants in the measuring chamber can cause erroneous readings. Before
using, make sure the measuring chamber is clean; if not, carefully wipe away all contaminants
with a cotton swab or gauze/soft cloth.
●Do not swing the device by its strap. This could damage/loosen the water-resistant packing in the
device.
●You may be subjected to penalties to use this device in the country not authorizing the device
usage due to its restrictions on wireless use.
●Communications errors or reductions in communication speed may result when using the device
in the proximity of a 2.4GHz wireless LAN. Keep the device and the communicating tablet as close
to each other as possible to avoid this.
CAUTION
CAUTION
IMPORTANT
SAFETY NOTES
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CONTENTS
SAFETY NOTES ............. 2
DEVICE FEATURES .................................................................................................................. 5
HARDWARE PACKAGE .................................................................................................................. 5
HARDWARE COMPONENTS .................................................................................................................. 6
BATTERY INSERTION / APP. INSTALL
.................................................................................................................. 7
TAKING MEASUREMENTS ............. 8
1. Using the Top Screen..........................................................................................10
2. Wireless Connection............................................................................................11
3. Graphing the Standard Curve............................................................13
3 -1 Measurement Wavelength Range Selection
......13
3 -2 Blank Measurement......................................................................14
3 -3 Measuring Known Concentrations....................16
3 - 4 Graphing the Standard Curve....................................18
3 -5 Standard Curve Loading Function ...................19
3 - 6 Unit Maintenance Function .......................................... 20
3 -7 Other Functions..................................................................................23
4. Taking Measurements...................................................................................... 25
4 -1 Manual Measurement.............................................................. 26
4 -2 Timed Measurement
(Simplied Kinetic Measurement)...................... 27
5. Turbidity Measurements............................................................................. 29
6. Displaying Previous Measurements....................................... 32
7. Saving Data (to a CSV le) ........................................................................34
OTHER SCREENS
8. Info Screen............................................................................................................................ 35
9. Maintenance Screen........................................................................................... 36
10. Help Screen.....................................................................................................................38
MAINTENACE .............................................................................................................. 39
TROUBLESHOOTING ..............................................................................................................40
WARRANTY AND AFTER-SERVICE .............................................................................................................. 42
SPECIFICATIONS .............................................................................................................. 43
LAWS AND REGULATIONS ..............................................................................................................44
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HARDWARE PACKAGE
The standard package includes:
(1) PAS-110-YU Unit ...................................................... 1
(2) Quick Start Guide (includes warranty)............. 1
DEVICE FEATURES
・ The PICOEXPLORERTM is a portable absorptiometer that uses a white LED as its light source.
・
You can verify PICOEXPLORERTM measurement results on your Smartphone or tablet PC by downloading a special
application that uses Bluetooth communication to check the results of measurement.
・
The high sensitivity yet extremely compact design of this device is the result of breakthrough Silicone Optical
Technology (SOTTM).
・
Battery-driven and portable.
・
Measurements can be taken directly from unopened PCR tubes, helping to prevent contamination.
DEVICE FEATURES HARDWARE PACKAGE
PA S-110 -YU U nit
Quick Start Guide (includes warranty)
Quick Start Guide
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
＿＿＿＿＿＿＿＿＿
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HARDWARE COMPONENTS
Measurement Chamber Cover
Open this cover to insert PCR tube
for sample measurement.
Power Switch
Turn unit on and o.
Battery Cover
Open to insert batteries.
Status Light
●Steady Red: check battery level
●Steady Purple:
powered and connected via
Bluetooth Low Energy
●Blinking Red: Low voltage*
●Blinking Blue: measurement in progress
* Some batteries may fail without rst blinking.
USB Interface Connector
(micro USB)
For connection with a USB cable.
Can be used to supply power
instead of batteries. Not a data
communication channel.
HARDWARE COMPONENTS
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BATTERY INSERTION
・ The unit does not come with ready-to-use, fully charged batteries.
Batteries Needed: 3 AAA Type Dry-Cell Batteries
CAUTION Use only AAA type batteries. Use of other types may damage the unit.
Follow the 3 steps below when replacing batteries.
1. Open the battery cover on the back side of the unit.
2. Insert batteries, aligning the plus and minus (+ and - )
ends of the batteries with the plus and minus markings
on the inside of the battery case.
3. Close the battery cover.
APP INSTALL BATTERY INSERTION
CAUTION
Make sure battery cover is securely fastened. A loose cover may allow water to seep into the
unit and cause damage.
Before opening the cover, wipe off any liquid on the outside to prevent see page and possible
damage.
＋＋
－
－＋
－
APP INSTALL
Please download and install the free app via Google Play or App Store.
App download procedure
1. Please open Google Play or App Store.
2. Type “PAS110” in the search box to use the search.
3. Tap on the left icon and conrm the content. Tap on “install” or “obtain” and follow the
instructions to install the app.
4. Wait until the “download and installation are complete.” Tap on “open” to start the app.
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Top Screen Connection Setting Screen
TAKING MEASUREMENTS
Follow the procedure outlined below for taking basic measurement readings.
1. Turn on unit power. The status light will turn red ●.
* If battery level falls too low, the red light will begin blinking.
2. Open the measurement chamber lid and set PCR tube into the measurement chamber.
Firmly close the lid.
3. Launch the tablet application
(PAS-110) by tapping the icon.
Page10, 1. Using the Top Screen.
4. Make BLE connection.
Page11, 2. Wireless Connection.
Note that the tube lid
connector should fit snugly
between the two guide
projections at the back of
the chamber.
CAUTION
Do not let any sample liquid from the PCR Tube spill into the unit.
This could damage the optical unit.
Guides
Connector
TAKING MEASUREMENTS
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Drawing Standard Curve Screen
Concentration Measurement Screen Measurement History Screen
Standard Curve Graph Screen
6. Measure concentration of the unknown
sample.
Page25, 4. Taking Measurements
7. Check previous measurements.
Page32, 6. Displaying Previous Measurements
5. Draw a standard curve.
Page13, 3. Graphing the Standard Curve
TAKING MEASUREMENTS
TAKING MEASUREMENTS
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Illustration 1: Top Screen
Draw Standard Curve
Button
Goes to the Draw
Standard Curve Screen
to allow creation of a
standard curve <P13>.
Turbidity Measure
Button
Goes to the Turbidity
Measure Screen
to take turbidity
measurements <P29>.
Measurement
Button
Goes to the
Measurement Screen
to take measurements
<P25>.
Help Button
Goes to the Help
Screen <P38>.
Connection
Setting Button
To connect to the
hardware unit, goes to
the connection setting
screen<P11>.
Return Button
Go back to previous screen.
* Android only
* With iOS (on iPhone or iPad) a Back
button is located in the upper left
corner of the screen.
Home Button
Returns to the Home Screen.
* Android only
Maintenance Button
Moves to the
Maintenance Screen
<P36>.
On this screen you can
also switch display
languages between
English and Japanese.
Bluetooth
Connection
Status
Unconnected
Battery
Voltage Level
OK
Battery
Voltage Level
Low
TAKING MEASUREMENTS
1. Using the Top Screen
The top screen displays the following buttons.
Information Button
Goes to the Info Screen
to show current device
status <P35>.
Measurement
History Button
Goes to the
Measurement History
Screen to allow
inspection of previous
measurements <P32>.
TAKING MEASUREMENTS
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Illustration 2-1: Connection Setting Screen
③
Bluetooth Device Address
Shows the last Bluetooth device
connected to.
②Connect Button
Press to connect.
① Device Search Button
Search for devices to
connect to.
TAKING MEASUREMENTS
2. Wireless Connection
Tap the Connection Setting Button on the top screen to move to this screen (Illustration 2-1).
* Make sure that Bluetooth functionality is enabled on your Smartphone or tablet PC.
CAUTION
If you are using an Android device, it is necessary to turn on access permission to the location of the app
and location service of the device. When these are OFF, you can not connect to the device.
TAKING MEASUREMENTS
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Illustration 2-2:
Device Address Not Found
Illustration 2-3:
Device Address Found
Illustration 2-4: Device Address Selected
（２） Tap the device address that you wish to connect to.
The background color of the address will change, indicating selection.(Illustration 2-4).
（ ３ ） Tap the ②Connect Button to begin connecting.
（ ４ ） Once the connection is established, tap the Return Button to return to the top screen.
TAKING MEASUREMENTS
（ １ ） Tap ①Device Search Button to search for photo absorbance sensor unit. If not detected, turn the photo absorb-
ance sensor off and on, then tap the ①Device Search Button again. If the photo absorbance sensor is found, its
Bluetooth device address will be displayed as shown in Illustration 2-3.
* (iOS only) "PAS-110" is displayed for the rst time. The address will be displayed from the second time.
TAKING MEASUREMENTS
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Illustration 3-1: Draw Standard Curve Screen ①
③ Input Percentage of
LED Output Level
⑤ Measurement
Button
TAKING MEASUREMENTS
3. Graphing the Standard Curve
When drawing a standard curve, first prepare a set of samples of known concentration.
The procedure is as follows:
1. Measurement wavelength range selection;
2. Blank measurement;
3. Measure known concentration sample data;
4. Verify the standard curve graph.
CAUTION
Before beginning the procedure, be sure to tap the Clear Button ( 3-7 ( 1 ) ) to clear previous data.
Tap the Standard Curve Button on the top screen to move to the Draw Standard Curve Screen (Illustration
3-1).
② Wavelength Range
Conrm Button
3-1. Measurement Wavelength Range Selection
（ １ ） Select the wavelength range for the sample to be measured using the ①RGB Switch Buttons.
（ ２ ） You can check the Maximum absorption wavelength and wavelength range by tapping the
②Wavelength Range Conrm Button of hint icon.
①RGB Switch Buttons
④ Blank Measurement
Intensity Display
(Red dotted lines show
where up to 3 samples
can be input.)
TAKING MEASUREMENTS
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Illustration 3-2:
Scrolling list of LED output levels
Illustration 3-3:
Baseline data input box selected
TAKING MEASUREMENTS
3-2. Blank Measurement
（ １ ） Set the sample for determining the blank
concentration into the measurement chamber.
（ ２ ） The ③LED output level is 10% by default, but other
levels can be selected. Select the LED output level
as appropriate according to blank measurement timing.
We recommend first taking a measurement at the 10%
default level, and then making adjustments later
<Page 15(5)>.
Tap the ③, the scrolling list (Illustration 3-2) is displayed.
Then select the number and tap the OK button.
（ ３ ） Do blank measurement. When you tap the ④Blank
Measurement Intensity Display, a black frame ap-
pears as shown in Illustration 3-3.
TAKING MEASUREMENTS
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Illustration 3-4:
Measured value displayed in white font
Illustration 3-5:
Data Clear Conrmation Notice
TAKING MEASUREMENTS
（ ４ ） Blank measurement begins when you tap the
⑤Measure Button. The measured blank intensity is
displayed in the black frame, and the absorption value
(0.000) is displayed in the frame on the right (Illustration
3-4).
At this time R, G, and B data are simultaneously meas-
ured. When measurement is finished, the value will be
displayed in white font. This data will be used to make
the graph of the curve <Page 18> .
*The measured intensity indicates the amount
of light passing through the sample.
Absorption is calculated based on the blank measurement
intensity and the measured intensity of the sample.
（ ５ ） The blank measurement intensity should fall in
the range of 30000 to 60000. If the value is lower,
raise the LED output level<Page 14(2)>.Also, tap
the ①RGB Switch Buttons and make sure that the
blank measurement intensity values for R, G, and B
are not all 65535. Values of 65535 indicate that the
measurement data is saturated, so absorption cannot
be properly measured.
（ ６ ）
If the measured intensity is 65535, return to ( 2 ), lower
the LED output level, and then measure again. When
the level is changed, a prompt like that shown in
Illustration 3-5 is displayed to request confirmation
that data is to be cleared; select “Yes”.
This completes blank measurement.
TAKING MEASUREMENTS
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Illustration 3-6: Draw Standard Curve Screen ②
TAKING MEASUREMENTS
3-3. Measuring Known Concentrations
Using the same Draw Standard Curve Screen (Illustration 3-6), measure a series of samples of known concen-
trations.
（ １ ） Set a sample into the measurement chamber of the photo absorbance sensor.
①Concentration Units
Select Button
②
Known Concentration
Input Area
(Always enter
concentration values in
ascending order in
Input Areas 1→5.)
③ Known Concentration
Sample Measurement
Intensity Display
(Up to 3 measurements
can be made on the
sample, displayed inside
the dotted red line.)
⑥
Standard Curve
Graph Display
Button
⑤ Absorbance Value
Display
(Blue outline:
values multiplied
by an absorbance
multiplier)
④ Measurement
Button
TAKING MEASUREMENTS
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Illustration 3-7:
Selection of Concentration Units
Illustration 3-8:
Results of Standard Curve
Measurement Procedure
TAKING MEASUREMENTS
（ ２ ） Tap the ①Concentration Units Select Button to
choose the units in which the measurements will be
made (Illustration 3-7). Tap the OK to confirm.
Initially, four unit variations are registered (mg/mL, μg/
mL, ng/mL, and %). See <Pages 20-22> for how to add
new units.
（３ ） Tap the ②Known Concentration Input Area and
input the known concentration value.
（４）
Tap the ③Known Concentration Sample
Measurement Intensity Display to display the black
frame.
（ ５ ） Tap the ④Measure Button to begin measurement.
The measured value will be displayed in white font.
（ ６ ） The ⑤Absorbance Value Display will show the
actual calculated absorbance.
（ ７ ） When this measurement is finished, remove the cur-
rent sample in the photo absorbance sensor, place
the next sample in, return to step ( 1 ), and repeat the
process.
（８）
When all the samples have been measured, tap
the ⑥Standard Curve Graph Display Button and
confirm the graphed results. The values displayed
in white font (Illustration 3-8) are the ones displayed
on the standard curve <Page 18>. If you wish to a
measurement from the graph, remove it by a long
press on that data’s display box.
（ ９ ） When you return to the top screen and proceed to
“Measure”, the unknown sample is measured using
the created standard curve <Page 25>. The standard
curve data is stored together with the measurement
data <Page 34>.
* The standard curve cannot be saved by itself.
TAKING MEASUREMENTS
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Illustration 3-9:
Standard Curve Graph Screen
Illustration 3-10:
Fitting a Line to the Measured Data Points
② Determination Coecient
Display Area
③RGB Switch Buttons
（ ２ ） When you tap the ① Standard Curve Graph Switch Button, graph displayed changes to show
a least squares linear regression line for the data (Illustration 3-10). Also, the coefficient of
determination (R2) is displayed at ②. This value indicates the linearity of the data how well the least
squares line ts the data is indicated by this value.
① Standard Curve Graph
Switch Button
TAKING MEASUREMENTS
3-4. Graphing the Standard Curve
Here we explain how to check the graph of created calibration curve.
（ １ ） When you tap the Standard Curve Graph Display Button, a graph appears indicating concentration
on the horizontal axis and absorption on the vertical axis (Illustration 3-9).
（ ３ ） All of the RGB graphs appear in the initial display. Graph display of each of the RGB graphs can be
switched on or off using the ③RGB Switch Buttons.
TAKING MEASUREMENTS
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TAKING MEASUREMENTS
3-5. Standard Curve Loading Function
Here we explain how to recall stored standard curve data that was used during earlier measurement for
reuse.
（ １ ） Tap the ①Select Standard Curve
Data to display the standard curve
data selection screen.
（ ２ ） Select the standard curve data and tap the ①Load button. The data loaded from the CSV le is
displayed on the standard curve creation screen.
If the added unit is unavailable after saving the standard curve data, “mg/mL” will be selected.
If you add the unit again on the unit maintenance screen, the unit will be available.
* If you load a standard curve created on another device, there is possibility not to perform accurate measurement.
①Select Standard
Curve Data
②Select Standard
Curve Data
①Load Button
TAKING MEASUREMENTS
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LAWS AND
REGULATIONS
TAKING MEASUREMENTS
3-6. Unit Maintenance Function
Here we show how to add new units in addition to the ones that are registered by initial setting (mg/mL, μg/
mL, ng/mL, and %).
How to add a unit
（ １ ） Tap the Unit Maintenance
Button on the standard curve
creation screen to display the
unit maintenance screen.
（ ２ ） Tap the Add Line Button on the unit maintenance screen to display the input line (maximum 10 lines).
Unit Maintenance Button
Add Line Button
TAKING MEASUREMENTS
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