Zeiss Colibri.2 User manual

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Illumination System Colibri.2 Operating Manual
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Carl Zeiss Copyright / Trademarks Colibri.2
2 423052-7244-001 07/2014
Knowledge of this manual is required for the operation of the device. You are therefore requested to
familiarize yourself with its content and pay special attention to the instructions regarding the safe
handling of the device.
We reserve the right to make changes in the interest of further technical development. This manual is not
subject to an update service.
© Unless expressly authorized, forwarding and reproduction of this document, as well as the
utilization and communication of its contents are not permitted. In the event of failure to comply
with this requirement, the violating party will be held liable for damage.
All rights reserved in the event of a patent approval or utility model registration.
All company and product names mentioned in this operating manual may be trademarks or registered
trademarks. Reference to non-company products is made for information purposes only and constitutes
neither an approval nor a recommendation.
Carl Zeiss Microscopy GmbH assumes no liability for the performance or use of such products.
Issued by: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[e-mail]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Number of this manual: 423052-7244-001
Date of issue: Version 2 - 07/22/2014
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Colibri.2 Contents Carl Zeiss
07/2014 423052-7244-001 3
CONTENTS
Page
1INTRODUCTION................................................................................................................ 5
1.1 Notes on device safety........................................................................................................ 5
1.2 Warning labels ................................................................................................................... 9
1.3 Warranty notes................................................................................................................. 11
2DESCRIPTION.................................................................................................................. 12
2.1 Name, intended use, and main features ............................................................................ 12
2.2 Components overview...................................................................................................... 13
2.3 Technical data .................................................................................................................. 14
3START-UP ....................................................................................................................... 15
3.1 Unpacking the illumination system.................................................................................... 15
3.2 Attaching the lamp module, connecting the control unit and the control panel .................. 16
3.3 Connection diagram......................................................................................................... 17
3.3.1 Connection diagram for stand-alone operation.................................................................. 17
3.3.2 Connection diagram with PC and motorized microscope for Fast Acquisition ..................... 18
3.4 Construction of the illumination system ............................................................................ 19
3.4.1 Colibri.2 lamp module ...................................................................................................... 19
3.4.2 Control unit ..................................................................................................................... 19
3.4.3 Control panel ................................................................................................................... 20
3.4.4 External white light source................................................................................................ 21
4OPERATION .................................................................................................................... 22
4.1 Switching on the control unit............................................................................................ 22
4.2 Functions of the control panel........................................................................................... 22
4.3 Functions of the white light source HXP 120 V .................................................................. 23
5MODIFYING THE LAMP MODULE .................................................................................. 24
5.1 Removing the lamp module from the microscope and opening it ....................................... 24
5.2 Inserting or changing LED modules ................................................................................... 25
5.3 Inserting or changing a mounting frame with beam combiner ........................................... 27
5.4 Inserting the beam combiner into the mounting frame or changing it ................................ 28
6CONFIGURATION AND OPERATION IN MTB AND AXIOVISION.................................... 29
6.1 Configuration of LED modules, beam combiners and excitation filters ................................ 29
6.2 Operation via the AxioVision Software .............................................................................. 31
6.2.1 Microscope - Reflected Light Work area ............................................................................ 31
6.2.2 Alternative operating menu with graphical representation ................................................. 32
7CONFIGURATION EXAMPLES OF THE LAMP MODULE ................................................. 34
8CARE, CHANGE OF FUSES, AND SERVICE...................................................................... 39
8.1 Care................................................................................................................................. 39
8.2 Changing the fuses of the control unit .............................................................................. 40
8.3 Service ............................................................................................................................. 40
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Carl Zeiss Contents / List of illustrations Colibri.2
4 423052-7244-001 07/2014
9ANNEX ............................................................................................................................41
9.1 Property rights ..................................................................................................................41
LIST OF ILLUSTRATIONS
Fig. 1-1 Warning labels on the Colibri.2 illumination system..............................................................9
Fig. 1-2 Warning labels and safety components on the Colibri.2 illumination system for laser
applications ......................................................................................................................10
Fig. 2-1 Components overview of the Colibri.2 illumination system .................................................13
Fig. 3-1 Attaching the illumination adapter.....................................................................................15
Fig. 3-2 Attaching the lamp module...............................................................................................16
Fig. 3-3 Connection diagram for stand-alone operation ..................................................................17
Fig. 3-4 Connection diagram with PC and motorized microscope for Fast Acquisition ......................18
Fig. 3-5 Construction of the lamp module ......................................................................................19
Fig. 3-6 Control unit, front.............................................................................................................19
Fig. 3-7 Control unit, rear ..............................................................................................................19
Fig. 3-8 Control unit, bottom .........................................................................................................20
Fig. 3-9 Control panel....................................................................................................................20
Fig. 3-10 Illumination system HXP 120 V, front.................................................................................21
Fig. 3-11 Illumination system HXP 120 V, rear ..................................................................................21
Fig. 4-1 Control unit ......................................................................................................................22
Fig. 4-2 Control panel, with lamp module being controlled.............................................................22
Fig. 4-3 Various indications on the control panel display when using the
Colibri.2 illumination system and the white light source HXP 120 V, respectively .................23
Fig. 4-4 Illumination system HXP 120 V, front.................................................................................23
Fig. 5-1 Loosening fastening screws ...............................................................................................24
Fig. 5-2 Removing the upper part of the housing............................................................................24
Fig. 5-3 Inserting an LED module into the lamp module ..................................................................26
Fig. 5-4 Changing a mounting frame with beam combiner .............................................................27
Fig. 5-5 Changing the beam combiner in the mounting frame ........................................................28
Fig. 6-1 Configuration via MTB2004...............................................................................................29
Fig. 6-2 Configuration via MTB2004...............................................................................................30
Fig. 6-3 AxioVision Software: Microscope - Reflected Light Tab Work area ......................................31
Fig. 6-4 Operating the LED modules in the illumination mode "Continuous" or "Gated" via a
separate operating menu ..................................................................................................32
Fig. 6-5 Operating the external white light source via a separate operating menu............................33
Fig. 8-1 Changing the fuses of the control unit...............................................................................40
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INTRODUCTION
Colibri.2 Notes on device safety Carl Zeiss
07/2014 423052-7244-001 5
1INTRODUCTION
1.1 Notes on device safety
The Colibri.2 illumination system has been designed, produced and tested in compliance with the
DIN EN 61010-1 (IEC 1010-1) standard - safety requirements for electrical measuring, control and
laboratory instruments. It meets the EC requirements 2006/95/EC - Low-Voltage Directive - and
2006/95/EC - Electromagnetic Compatibility. The device has to be disposed of in accordance with the
WEEE Directive 2012/19/EC.
It is marked with the sign.
The present manual contains information and warnings that have to be observed by the operator.
The warning and note symbols used in this manual are described below:
CAUTION
This symbol indicates possible danger to the operator.
WARNING of possible laser radiation!
The safety screws on the lamp module and Colibri.2 illumination adapter may only be removed
by Carl Zeiss Service (not a customer interface). Otherwise there is a risk of eye damage upon
laser coupling.
WARNING of possible laser radiation!
The safety ring on the fiber coupling of the HXP light source may not be removed (not a
customer interface). Otherwise there is a risk of eye damage upon laser coupling.
CAUTION LED radiation!
LED risk group 3 according to DIN EN 62471:2009 when the user looks directly into the
Colibri.2 light output.
UV radiation is emitted.
Any direct looking into the Colibri.2 light output is to be avoided.
Failure to heed this caution may result in eye damage.
Skin and eye irradiation is to be minimized by appropriate shielding.
CAUTION: High-energy UV radiation!
Risk of damage to the eyes and skin!
CAUTION
Disconnect the device from the line before any intervention!
ATTENTION
This symbol indicates possible danger to the device or device system.
NOTE
This symbol marks notes the operator must pay special attention to.
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INTRODUCTION
Carl Zeiss Notes on device safety Colibri.2
6 423052-7244-001 07/2014
The Colibri.2 illumination system is usually attached to the microscope stand.
Depending on the design of the microscope, the risk for the user must be assessed as being markedly
lower then.
When it comes to the intended use of the Axio Examiner microscope with Colibri.2, for example, the risk
status of the overall system is downgraded to LED risk group 2 according to DIN EN 62471:2009.
The Colibri.2 illumination system including its original accessories may only be used for the purposes
described in this operating manual.
Particular attention must be paid to the following notes:
Use the illumination system for Carl Zeiss microscopes only.
The manufacturer cannot assume liability for any other applications, including that of individual
modules or single parts. This also applies to any service or repair work that is not carried out by
authorized service personnel. All warranty claims shall be forfeited.
To ensure safe operation of the illumination system in connection with the microscope, please
pay attention to the operating manual of the microscope.
Do not operate the Colibri.2 illumination system in explosion-prone areas.
Keep combustible and easily inflammable materials away from the light beam and its
surroundings.
The Colibri.2 illumination system is not equipped with any special devices for protection from
substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that
could be hazardous to health. When handling such substances make sure that all legal
regulations, particularly the relevant national accident prevention regulations, are observed.
The power plug of the control unit must be inserted in an outlet featuring a grounding (earth)
contact. The grounding effect must not be made ineffective by an extension cable that does
not have a protective ground wire.
When protection measures are found to be no longer effe
ctive, the instrument must be
switched off and safeguarded against inadvertent operation. For repair please contact the Carl
Zeiss Microscopy Service in Germany (see page 40) or your local Carl Zeiss representative.
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INTRODUCTION
Colibri.2 Notes on device safety Carl Zeiss
07/2014 423052-7244-001 7
Before switching on the control unit please check whether it is suitable for the available line
voltage. Disconnect the power plug of the control unit before opening the device and before
changing the fuses!
Take care that only fuses for the rated power are used. Use of makeshift fuses and short-
circuiting of the fuse holders are not permitted.
The lamp module, if equipped with the corresponding LED modules or if a white light source
coupled by a liquid light guide is used, emits ultraviolet radiation that may cause burns to the
eyes and skin. Therefore, never look directly into the light and avoid direct incidence of the light
on your skin. When operating the microscope, always use th
e protective devices belonging to
the instrument.
Never look into the light beam – neither with nor without optical instruments, even not if you
simply want to observe the specimen. Your eyes may be damaged in case of non-observance!
Placing objects against or covering ventilation slats of the control unit may lead to heat build-up
that may damage the device and, in extreme cases, cause a fire. Always keep the ventilation
slats clear and ensure that no objects enter the device through the ventilation slats.
Dirt and dust may impair the performance of the device. Therefore, the device must largely be
protected from these influences and covered with the dust cover when not being in use. Before
covering the device, please make sure that it has been switched off.
Take appropriate protective measures if the specimen releases noxious gases, dust, and vapors,
secondary radiation or explosive substances as a result of the UV radiation!
If an external white light source (e.g. HXP 120 V) is used in connection with Colibri.2
, never
look directly into the light guide output of the white light source to avoid the risk of being
dazzled and going blind.
The white light source must not be switched on until after having connected the output of the
liquid light guide tightly with the Colibri.2 system.
When using the white light source HXP120 V
, please pay attention to the manufacturer's
operating instructions.
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INTRODUCTION
Carl Zeiss Notes on device safety Colibri.2
8 423052-7244-001 07/2014
Opening the Colibri.2 Controller housing is only permitted to authorized service personnel.
The bulb of the external white light source has to be changed according to the operating
instructions of the manufacturer of the white light source. Otherwise there is danger of burns
and explosion when changing the bulb.
Defective devices must not be disposed of with the household waste. Dispose them of in
compliance with the relevant legal requirements or return them to the manufacturer of the
device.
The manufacturer of the device is under the legal obligation to take defective devices back.
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INTRODUCTION
Colibri.2 Warning labels Carl Zeiss
07/2014 423052-7244-001 9
1.2 Warning labels
Standard labeling
Fig. 1-1 Warning labels on the Colibri.2 illumination system
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INTRODUCTION
Carl Zeiss Warning labels Colibri.2
10 423052-7244-001 07/2014
Labels on the lamp module and Colibri.2 illumination adapter for laser application.
Fig. 1-2 Warning labels and safety components on the Colibri.2 illumination system for laser
applications
The safety screws (Fig. 2-1/1 and 2) on the lamp module and Colibri.2 illumination adapter may
only be removed by Carl Zeiss Service (not a customer interface).
Otherwise there is a risk of eye
damage upon laser coupling.
The safety ring (Fig. 2-1/3) on the fiber coupling of the HXP light source may not be removed
(not a customer interface). Otherwise there is a risk of eye damage upon laser coupling.
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INTRODUCTION
Colibri.2 Warranty notes Carl Zeiss
07/2014 423052-7244-001 11
1.3 Warranty notes
The manufacturer guarantees that the device is free from material and manufacturing defects when
delivered. Any defects have to be reported to us immediately and everything possible has to be done to
minimize the damage. In the event that such a defect has been reported, the manufacturer is obligated
to remedy it to its discretion by repairing the defective device or by delivering a perfect one. No
guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper use.
The manufacturer of the device is not liable for damage caused by faulty operation, negligence or any
other tampering with the device, in particular as a result of removing or exchanging device components,
or the use of accessories from other manufacturers. This forfeits all claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair work to the
microscopes may be performed. Repairs are only allowed to the Carl Zeiss service staff or specially
authorized personnel. Should any defect occur, please contact first the Carl Zeiss Microscopy Service in
Germany (see page 40) or your local Carl Zeiss representative.
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DESCRIPTION
Carl Zeiss Name, intended use, and main features Colibri.2
12 423052-7244-001 07/2014
2DESCRIPTION
2.1 Name, intended use, and main features
Manufacturer's designation: Colibri.2 Illumination System
The Colibri.2 illumination system is designed to be used as a light source for reflected light fluorescence
applications. It can be used in connection with the upright microscopes Axio Imager, Axioplan 2,
Axioskop 2, Axioskop 2 FS, Axioskop 40, as well as with the inverted microscopes Axio Observer and
Axiovert 200.
Major features are:
−High-end LED light source for fluorescence applications with visible and UV light for direct
connection to the microscope
−Excellent contrast properties
−Up to four LED modules with different emission wavelengths can be used individually or combined
in the lamp module
−LED modules are exchangeable, at present ten different LED modules are available
−No alignment required
−LED modules are equipped with a chip for automatic component recognition (ACR)
−LED emission is individually adjustable for every LED in one-percent steps
−Very quick switching on, over and off of the LED modules, no shutter required
−Optoelectronic switchover between LED modules, no mechanical movements
−Very long life time
−Minimum heat development
−Changeable beam combiners for the configuration of the light path in the Colibri.2 lamp module
−Specific filter sets (as P&C filter modules and separate excitation filters) for the LED modules
deliverable
−Motorized interface for fiber coupling of an external white light source
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DESCRIPTION
Colibri.2 Components overview Carl Zeiss
07/2014 423052-7244-001 13
2.2 Components overview
Fig. 2-1 Components overview of the Colibri.2 illumination system
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DESCRIPTION
Carl Zeiss Technical data Colibri.2
14 423052-7244-001 07/2014
2.3 Technical data
Dimensions
Lamp module (length ×width ×height) ....................................................... 240 mm x 155 mm x 75 mm
Control unit (length ×width ×height)........................................................... 225 mm x 250mm x 95 mm
Control panel (length ×width ×height) ....................................................... 178 mm x 108 mm x 64 mm
LED module (length ×diameter)......................................................................................53 mm x 31 mm
Illumination adapter (length ×diameter)..........................................................................86 mm x 78 mm
Weight
Lamp module...................................................................................................................approx. 2400 g
Control unit .....................................................................................................................approx. 2600 g
Control panel.................................................................................................................... approx. 460 g
LED module ........................................................................................................................ approx. 75 g
Illumination adapter .......................................................................................................... approx. 550 g
Ambient conditions
Transport (in packaging):
Permissible ambient temperature....................................................................................-40 to +70 °C
Storage:
Permissible ambient temperature...................................................................................+10 to +40 °C
Permissible relative humidity (without condensation)............................................. max. 75 % at 35 °C
Operation:
Permissible ambient temperature...................................................................................+10 to +40 °C
Permissible relative humidity (without condensation)............................................. max. 75 % at 35 °C
Altitude of installation site...............................................................................................max. 2000 m
Atmospheric pressure .........................................................................................800 hPa to 1060 hPa
Pollution degree ...............................................................................................................................2
Operating data
Operating environment ........................................................................................................ closed room
Protection class ......................................................................................................................................I
Protection type................................................................................................................................ IP 20
Electrical safety............................................................in compliance with DIN EN 61010-1 (IEC 61010-1)
..............................................................................................................including CSA and UL directives
Overvoltage category.............................................................................................................................II
Radio interference suppression ........................................................in accordance with EN 55011 Class B
Noise immunity ....................................................................................in accordance with DIN EN 61326
Line voltage range of control unit ....................................................................100 to 240 V AC (±10 %)
Line frequency.......................................................................................................................50 to 60 Hz
Power consumption of control unit................................................................................................ 70 VA
Fuses according to IEC 60127
Control unit ................................................................................................T 2.5 A/H; 250 V; 5 X 20 mm
Colibri.2 overall LED classification: .......................... LED risk group 3 according to DIN EN 62471:2009
LED module 365 nm.................................................... LED risk group 3 according to DIN EN 62471:2009
LED module 470 nm.................................................... LED risk group 1 according to DIN EN 62471:2009
LED module 619 nm..................................................No LED risk group according to DIN EN 62471:2009
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START-UP
Colibri.2 Unpacking the illumination system Carl Zeiss
07/2014 423052-7244-001 15
3START-UP
The Colibri.2 illumination system consists of the following main parts:
−Colibri.2 lamp module
−Control unit
−Control panel
The device can be assembled by the customer.
The lamp module is completely mounted according to customers' order, including LED modules and
beam combiners. If necessary, the LED modules and beam combiners can be changed quickly and easily
by the customer (see Section 5).
3.1 Unpacking the illumination system
The lamp module, the control unit and the control
panel, as well as the optional accessories are
supplied separately in customary packaging.
Remove all parts from the packaging and check
them for completeness according to the delivery
note.
For use with confocal Laser Scanning Microscopes
the illumination system Colibri.2 (Fig. 3-1/1) is
delivered with tightly mounted illumination
adapter (Fig. 3-1/2).
For laser safety reasons the connection between illumination adapter and lamp module is no
customer interface. Disregard results in losing laser safety of the system.
The Colibri.2 lamp module can be only used with the upper part of its housing properly mounted. As
soon as the upper part of the housing is removed, a safety switch (interlock) will switch off the LED
modules.
Fig. 3-1 Attaching the illumination adapter
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START-UP
Carl Zeiss Attaching the lamp module, connecting the control unit and the control panel Colibri.2
16 423052-7244-001 07/2014
3.2 Attaching the lamp module,
connecting the control unit and the
control panel
The lamp module operates via the illumination
mount (reflected light) of the microscope.
•Attach the lamp module (Fig. 3-2/1) via the
dovetail of the illumination adapter to the
reflected light mount (Fig. 3-2/4) of the
microscope and fasten it by means of the
clamping screw.
The lamp module does not require any adjustment.
•Connect the lamp module cable (Fig. 3-2/2) to
the 25-pin subD port of the control unit (see
Fig. 3-7/4).
•Connect the connection cable of the control
panel (see Fig. 3-9/1) to the 15-pin subD port of
the control unit (see Fig. 3-6/2).
•Connect the control unit (see Fig. 3-7/2) with
the power cable to a power outlet.
An external white light source (e.g. HXP 120 V, see Fig. 3-10) can be connected at the coupling position
for liquid light guides as follows:
•Introduce one end of the light guide into the white light source (see Fig. 3-11/3) and the other end
into the connection point of the lamp module (Fig. 3-2/3) and fasten it by means of the swivel nut.
•The internal motorized shutter of the white light source HXP 120 V can be triggered via the 9-pin
subD port of the lamp housing. Connect for this purpose the trigger cable with the BNC socket OUT 1
of the Colibri control unit (see Fig. 3-7/1).
•Connect the white light source (see Fig. 3-11/2) with a power cable to a power outlet.
Fig. 3-2 Attaching the lamp module
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START-UP
Colibri.2 Connection diagram Carl Zeiss
07/2014 423052-7244-001 17
3.3 Connection diagram
3.3.1 Connection diagram for stand-alone operation
Fig. 3-3 Connection diagram for stand-alone operation
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START-UP
Carl Zeiss Connection diagram Colibri.2
18 423052-7244-001 07/2014
3.3.2 Connection diagram with PC and motorized microscope for Fast Acquisition
* Colibri.2 can be connected alternatively via USB or via CAN!
Fig. 3-4 Connection diagram with PC and motorized microscope for Fast Acquisition
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START-UP
Colibri.2 Construction of the illumination system Carl Zeiss
07/2014 423052-7244-001 19
3.4 Construction of the illumination system
3.4.1 Colibri.2 lamp module
The Colibri.2 lamp module can be equipped with
up to four LED modules (Fig. 3-5/1) and three
beam combiners (Fig. 3-5/2).
The LED modules and beam combiners can be
switched by the customer.
When using an additional fiber coupled white light
source, it is possible to toggle between the lamp
module and the external white light source by
means of a motorized built-in switching prism
(Fig. 3-5/3, covered).
3.4.2 Control unit
The control unit of the lamp module contains the
control and the power electronics.
At the front of the device there is the ON/OFF
switch Power (Fig. 3-6/1with pilot lamp), as well
as a 15-pin subD port (Fig. 3-6/2) for the control
panel.
The rear of the control unit contains:
−several BNC ports; the trigger cable leading
to the white light source has to be inserted
in OUT 1 (Fig. 3-7/1)
−the power connection socket (Fig. 3-7/2)
−two CAN ports (Fig. 3-7/3) for the
connection to the microscope
−the 25-pin subD port (Fig. 3-7/4) for
connecting the Colibri.2 lamp module
−the USB port (Fig. 3-7/5) for the connection
to a PC.
Fig. 3-5 Construction of the lamp module
Fig. 3-6 Control unit, front
Fig. 3-7 Control unit, rear
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