4titude 4s3 User manual

4titude Ltd. • The North Barn • Surrey Hills Business Park Damphurst Lane • Wotton • Surrey • RH5 6QT
T: [phone] • F: [phone] • E: [email protected] • www.4ti.co.uk
Semi-Automatic Sheet Heat Sealer 4s3™
Operation Manual
Version: 1.0
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0 •en1 •11.2018

4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
4titude Ltd.
The North Barn, Surrey Hills Business Park
Damphurst Lane, Wotton, Surrey, RH5 6QT
UK
Declaration of Conformity
Product Name: 4s3™ Semi-Automatic Sheet Heat Sealer
Model Name: 4s3™
This product complies with the following European standards:
• 2014/35/EU Low Voltage Directive
• 2014/30/EU Electromagnetic Compatibility Directive
• 2011/65/EU Restriction of the use of certain hazardous
• Substances in electrical and electronic equipment
• (RoHS) Directive
• 2006/42/EC Machinery Directive
Copyright Notice
No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical,
for any purpose, without prior written permission of 4titude®Ltd.

4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
Safety Information
This operation manual contains important operating and maintenance instructions which must be read, understood,
and followed by the product user. Failure to use this product according to this operation manual may degrade or
defeat the protection normally provided by this product.
Read this operation manual including the safety notes prior to product use and keep it for future reference.
This product must only be used in accordance with proper safety standards and procedures, together with the
instructions included in this manual.
The sealer has been designed and manufactured to conform to international safety specications.
It’s essential that the users know the potential hazards associated with the equipment.
All operators should know and observe the safety precautions and warnings given in this section before starting
to use the instrument.
If the unit is used in a way not specied by the manufacturer, the protection provided by the equipment may be
ineffective.
Symbol table
Please read carefully this table before using your unit. The following caution and safety symbols are used in this
manual.
Symbol Description
Warning - Refer to manual!
The machine is designed for indoor laboratory use only, at an altitude of less than 2200 m above sea level,
within a temperature range of 15°C to 35°C and a relative humidity range of 5% to 85% non-condensing.
If the instrument is stored outside these ranges, it should be left to stand until it equilibrates to within the
above limits.
Ensure that the voltage selection switch is set to the correct voltage and that the correct fuse for the required
voltage setting is tted.
Do not work outside the rated power supply range.
Use the cleaning method recommended by the manufacturer.
Ensure the unit is only connected to an earthed supply.
There are no user accessible or serviceable parts inside the unit. Do not remove the unit’s cabinet.
Warning - Risk of electrical shock (high voltage)
This equipment should only be dismantled by properly trained personnel, removing the top case exposes
potentially lethal mains voltages.
Warning - Hot surface
The hot plate can reach temperature up to 200°C,
Care must be taken not to touch it or serious burns may occur.
The equipment will maintain a high temperature for a considerable time after switching off and it is essential
to wait before starting cleaning operations.
Sealed sample plates may remain hot for some seconds after sealing and should be handled with care
Warning - Indicates pinch, crush and cut hazard
Biohazard - Indicates the possible presence of biological or hazardous substances

4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
Warnings
Personal Injury
• Do not use this product in a manner other than as stated in the Operating Conditions section of this manual as
the protection provided by the equipment may be impaired.
• This product is designed for use in laboratory environments by persons knowledgeable in safe laboratory
practices.
• Always wear safety glasses and other appropriate protective equipment when operating this product.
Electric Shock
• This product must be connected to a grounded power outlet for safe functioning.
• Use only the power cord supplied with the product.
• The power cord is the device available for full disconnect from mains input.
• Position the product for use so that the power cord can be easily disconnected without having to move the
product.
• Disconnect the power cord before moving or cleaning the unit.
Product Damage
• Keep the product dry and clean.
• Do not immerse the product in liquid for cleaning.
• This unit is not explosion or spark proof.
• Do not operate this product near volatile or ammable materials.
General Operation Safety
• When using infectious, radioactive, toxic and other solutions which may pose health risks, please observe the
appropriate safety precautions.
• Do not use this machine in a potentially explosive environment or with potentially explosive chemicals.
• Install the machine in a location free of excessive dust.
• Avoid placing the machine in direct sunlight.
• Choose a at, stable surface capable of withstanding the weight of the machine.
• Install the machine in the room temperature 10 ‒ 30˚C, relative humidity 0 ‒ 85%.
• Do not block the air vents.
• Make sure the power source conforms to the required power supply specications.
• To avoid electric shock, make sure the machine is plugged into a grounded electric outlet.
• Do not allow water or any foreign objects to enter the various openings of the machine.
• Switch off the power switch before cleaning or performing any service on the machine, such as replacing the
fuses.
• To guarantee sufcient ventilation, ensure that the sealer has at least 30 cm of free space on all sides, including
the rear.
• Repair should be carried out by authorized service personnel only.
• Use original spare parts and accessories only.

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
Table of Contents
1 4s3™Introduction ..........................................................................................................................8
1.1 Unpacking (Packing / Contents Listing) ……………………………………………………………………………8
1.2 Instrument Overview …………………………………………………………………………………………………9
2 Installation .....................................................................................................................................10
2.1 Unpacking and Start-Up…………………………………………………………………………………………… 10
2.2 Voltage Check ……………………………………………………………………………………………………… 11
3 Settings and Operation ................................................................................................................12
3.1 Set-Up Operating Mode …………………………………………………………………………………………… 12
3.2 Time and Temperature Settings ………………………………………………………………………………… 12
3.3 Stand-by, Power Down and Audible Warning Control Settings ……………………………………………… 12
3.4 Operation - "Run" Mode …………………………………………………………………………………………… 13
3.5 Operation - "Stand-by" Mode …………………………………………………………………………………… 13
3.6 "Power-down" Mode ……………………………………………………………………………………………… 14
3.7 Closing Drawer …………………………………………………………………………………………………… 14
4 Optimising Seal Quality................................................................................................................14
4.1 Plate Requirements ……………………………………………………………………………………………… 14
4.2 Plate Support Adapters …………………………………………………………………………………………… 15
4.3 Heat Seal Materials………………………………………………………………………………………………… 16
4.4 Sealing Parameters ……………………………………………………………………………………………… 16
5 Troubleshooting............................................................................................................................18
5.1 Error Messages …………………………………………………………………………………………………… 18
5.2 Cleaning the Heating Platen ……………………………………………………………………………………… 18
6 Maintenance ..................................................................................................................................19
7 AppendixA:TechnicalSpecications ........................................................................................20
8 Appendix B: Ordering Information..............................................................................................21
9 Appendix C: Warranty ..................................................................................................................22
10 Appendix D: Shipping Instructions.............................................................................................23
11 Appendix E: Recycling and Disposal..........................................................................................24
11.1 Uninstalling Instructions …………………………………………………………………………………………… 24
11.2 Disposal Instruction………………………………………………………………………………………………… 24

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4s3™ Semi-Automatic Sheet Heat Sealer
a4S Automatic Heat Sealer, Operation Manual V2.8
1 4s3™Introduction
The 4s3™ is a compact semi-automatic sheet heat sealer which is compatible with a wide range of seals and
plates of differing designs and heights. With variable temperature and time settings, sealing conditions are easily
optimised to produce a 100% tight seal, eliminating sample loss.
Plate and seal are placed on the holder, the "Operate" button is pressed and the drawer automatically closes. The
sealing process is controlled by an electric mechanism providing consistent plate sealing, eliminating variation
and giving reliable sealing every time.
When using the 4s3™ for the rst time, please read this entire operation manual carefully. To guarantee problem
free and safe operation, it is essential to observe the following information.
1.1 Unpacking (Packing / Contents Listing)
The device is delivered in an external carton and an internal carton with protective PE foam cushions. Remove the
4s3™ from each carton. All packaging should be retained until it has been established that the 4s3™ is working
properly.
Open the Semi-Automatic Heat Sealer package (4ti-0655) and conrm that all items are included:
• 4s3™ Device
• Power Cable
• Operation Manual and Quick User Guide
• 4s3™ Plate Support Adapter, Standard (4ti-0615)
If there are any items missing, damaged, or not according to your order, please contact your distributor or sales
representative immediately.

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
1.2 Instrument Overview
1.2.1 Sealer Parts
Casing and Control Panel Rear Power Interface
Heating Platen Plate Carrier
1.2.2 Control Panel
Operate, Open Drawer &
Incremental (up) Key
Three digit LED displaySet-up/Run Mode Key
Amber LED Status
Indicator
Close Drawer &
Decremental (down) Key
1.2.3 Rear Power Interface
Power Setting 115/230 Vac
and Fuse Holder
I/O Power Switch
Mains Power Plug

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4s3™ Semi-Automatic Sheet Heat Sealer
a4S Automatic Heat Sealer, Operation Manual V2.8
2 Installation
2.1 Unpacking and Start-Up
After unpacking retain all packing material and xtures, as the unit must always be transported in its original
packaging to avoid damage.
The manufacturer accepts no responsibility for damage incurred unless the unit is correctly packed and transported
in this way.
Remove the 4s3™ unit from its packaging. Insert the power cable and power up the instrument. Once powered
up, press and the drawer will open. Remove the protective elastic bands and press .
The unit has to be placed on a level surface, away from direct sunlight ensuring access to the power switch at the
back of the unit.
Ensure that the vents on the casing are not obstructed.
The mains voltage selector switch should be checked to ensure that the voltage has been set to the correct value.
Connect the unit to the mains power supply and switch on the I/O switch on the rear power interface.
If the heater within the unit is receiving power the amber LED on the control panel will be illuminated.
After setting the desired temperature, the temperature display value will increase and the LED light will ash. Wait
until the amber LED light stops ashing and an audible “beep” is heard. This signals to the operator that the set
temperature has been reached and the unit is ready to seal.

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
2.2 Voltage Check
Be sure that the equipment is not connected to the mains power.
At the rear of the 4s3™ is the connection panel with:
• Power setting 115/230 Vac and Fuse Holder
• I/O Power Switch
• Mains Power Plug
The set voltage is displayed in white on a red background
on the power setting and fuse holder.
Changing the Voltage Setting
1. Open fuse holder compartment with a screwdriver.
2. With the screwdriver lever out the fuse holder.
Opening fuse holder
compartment
Levering out
fuse holder
3. Replace the two fuses according to the table below:
Power Voltage (EN 60127)
115 V 3.15 A (T)
230 V 2 A (T)
4. Insert the fuse holder keeping the set voltage label at the top.
5. Close the fuse compartment by pressing gently.
6. If the fuse holder is inserted correctly you can read the voltage selected
Correct fuse location Incorrect fuse location

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4s3™ Semi-Automatic Sheet Heat Sealer
a4S Automatic Heat Sealer, Operation Manual V2.8
3 Settings and Operation
3.1 Set-Up Operating Mode
Turn on the 4s3™ (switch located at back of the unit). The display will show the software version for a couple of
seconds and then show the temperature to which the 4s3™ is currently set. The unit is delivered with the heater
platen turned off. If this is the rst time of switching the unit on, the keypad will display “OFF”. Otherwise, when
switched on, the unit will start to heat up to the last set temperature and the LED light will ash whilst it is heating up.
3.2 Time and Temperature Settings
• Briey press and release
S
• The display shows the last set sealing temperature (ashing)
• The temperature can be decreased or increased using / .
• Temperature can be set between 120°C and 200°C with steps of 1°C or to “OFF”. Pressing the keys once,
increases or decreases the temperature in 1°C steps. Pressing and holding your nger on the keys, rapidly
cycles through the values. Release the button once you are on the desired temperature.
• Press
S
again to store the new set temperature and then the display will ash with “t” and the current sealing
time (ashing).
• The sealing time can be decreased or increased using / .
• Time can be set between 0.0 and 1.0 second in 0.1 second increments and from 1-10 seconds in 0.5 second
increments.
• Press
S
again to store the new sealing time value. In doing so, you will also exit from the programme menu.
• The unit returns into “run mode” (please refer to section 3.4, page 13) and the actual temperature of the
heating platen is shown in the display.
3.3 Stand-by, Power Down and Audible Warning Control Settings
• Press
S
for 10 seconds, then release.
• The display shows the symbol “L” (ashing) and the last set stand-by time in minutes.
The stand-by time is the time after which the heater temperature is automatically reduced to 60°C when the unit
is left idle.
• The stand-by time can be decreased or increased using / .
• Time can be set between 1 minute and 99 minutes with steps of 1 minute or as “no” standby.
• Press
S
again to store the new stand-by time and then the display will ash with “P” and the current power-
down time (in hours).
The power-down time is the time after which the heater is switched off automatically when the unit is left idle.
• The power-down time can be decreased or increased using / .
• Power-down can be set between 1 hour and 24 hours with steps of 1 hour
• Press
S
again to store the new power-down time and the display will ash with “S” and the current audible
warning setting.
• The audible warning function can be enabled (“on”) or disabled (“no”) / .
• Press
S
again to store the audible warning function and the display will ash with “r” this MUST BE set to
“on”, otherwise the drawer will not function correctly. Press
S
again to save and exit.
• The unit returns into “run mode” (please refer to section 3.4, page 13) and the actual temperature of the
heating platen is shown in the display.

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
3.4 Operation - "Run" Mode
• Switch on the unit. The unit will automatically begin to warm up the heating platen to the last set temperature.
The display shows the temperature increase in real time with the amber LED ashing.
• When the heating platen reaches the set temperature an audible beep is emitted and the amber LED remains
permanently illuminated.
• The system is ready for sealing.
• Press the “OPERATE” (UP) button to open drawer and then the sample plate can be loaded onto the appropriate
plate support adapter, please refer to section 4.2.
• Place the sealing lm over the plate, ensuring that the sealing surface is face down.
Note:
When using heat sealing lms that have a tendency to curl, e.g. 4titude® Peel Heat Seal (4ti-0521), DMSO
Resistant Peel Heat Seal (4ti-0587) and Clear Weld Heat Seal Mark II (4ti-0575), use the 4s3™Sealing Frame
(4ti-0612) to hold down the seal.
The Sealing Frame is supplied with the 4s3™ Plate Support Adapter, PCR 96 (4ti-0625, optional extra) and
also available as separate item (4ti-0612).
For deep well plates place the 4s3™ Weighted Sealing Platen (4ti-0602, optional extra) on top of the sealing
lm.
For most other plates we recommend the use of the 4s3™Sealing Frame (4ti-0612).
• Press the “OPERATE” (UP) button with drawer open, plate and adapter loaded. The drawer will automatically
close and the sealing process will begin.
• Once the plate makes contact with the heating platen the display shows the symbol “t” and the countdown of
the programmed sealing time starts.
• When the countdown timer reaches zero the drawer automatically opens. The sealed plate can now be removed.
• The unit emits an audible beep once the heating platen has reached the programmed temperature again and a
new cycle can be started.
Note: Foil backed lm may remain hot for a number of seconds after sealing. Care should be taken in handling
these plates.
3.5 Operation - "Stand-by" Mode
If left idle for a prolonged time the sealer will automatically switch into stand-by mode (factory set at 60 minutes)
and the temperature of the heating plate will be reduced to 60°C.
When entering into stand-by mode the display shows the decrease of the temperature in real time and the amber
LED is switched off.
The temperature of the heating platen will be maintained at 60°C during standby.
The display shows the actual temperature of the heating platen and not the set sealing temperature.
By pressing any key the unit will automatically begin to warm up the heating plate to the pre-selected sealing
temperature.
Refer to section 4.4 to change the time after which the unit switches into stand-by mode or to switch off the stand-
by function.

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4s3™ Semi-Automatic Sheet Heat Sealer
a4S Automatic Heat Sealer, Operation Manual V2.8
3.6 "Power-down" Mode
If the unit remains idle in stand-by mode for a prolonged time the system will go into power down mode and the
heating platen will be switched off (factory set at 10 hours). The amber LED is switched off.
The display shows the temperature decrease until it reaches 45°C, after this “LO” will be displayed.
By pressing any key the unit will automatically begin to warm up the heating platen to the pre-selected sealing
temperature.
Refer to section 3.3 to change the time after which the unit switches into power-down mode.
3.7 Closing Drawer
When the unit is not in use, to prevent damage or dust contamination the drawer can be closed by pressing the
“CLOSE” (DOWN) button.
WARNING! Do not close the drawer by hand
4 Optimising Seal Quality
4.1 Plate Requirements
The 4s3™ accommodates plates made from a variety of materials and plate designs. For a complete list of the
acceptable sealing materials, please reder to section 4.3, page 16.
The 4s3™ is designed to accommodate plates which meet the standard established by the Society of Biomolecular
Sciences (SBS). These include but are not limited to deep-well plates, PCR and standard microplates in the 96,
384 and 1536 well formats.
In accessing the suitability of a plate for heat sealing, it is important to look at its design and the quality of
manufacturing. In general terms, it is important for the plate design to have raised rims around each well, giving a
dened sealing ring around each well. When assessing the seal quality, it is important to study a recently removed
seal looking for a regular pattern of sealing rings from one end of the plate to the other.
Incomplete sealing, indicates that the plate is either not perfectly at (or not attened during the sealing process)
or that the plate is not supported adequately in the plate support adapter.
The 4s3™ is designed to be used with any of the following plate types made from polypropylene, polyethylene,
polystyrene, polycarbonate and COC.
• PCR plates
• SBS format shallow well plates (384-well)
• Roche 1536 well plates
• Deep-well plates
• Tube blocks (various)
• Vision Plates™

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
4.2 Plate Support Adapters
The 4s3™ is designed to seal microplates from 4 mm to 60 mm in height. All plates must rst be placed in an
appropriate adapter to bring the sealing surface into the correct height range for the sealer. In addition to this, 96
and 384 well PCR plates require an adapter to support the plate precisely enough to produce an even seal. With
a 96 and 384 well PCR plate it is necessary to support each well completely, whereas with standard microplates,
it is only necessary to support the underside of the well but not the skirt.
Our Random Access plates supply a novel 96 well plate design with individually removable tubes. These plates
can be sealed in one step using Random Access heat seals - 96 individual foil seal spots on a removable backing
- resulting in individually sealed tubes. Please refer to the table to select the correct adapter for the plate you wish
to seal.
Plate Type Adapter to use
Fully skirted PCR plates
(e.g. 384 well and low prole
96 well PCR Plates, fully skirted
FrameStar®PCR plates)
Plate Support Adapter, Standard (4ti-0615, included) 4ti-0615
96 well PCR plates Plate Support Adapter, PCR 96 (4ti-0625, optional extra;
includes 4s3™ Sealing Frame 4ti-0612)
Note: When using the PCR 96 adapter, the standard plate
adapter (4ti-0615, included) should be removed from the drawer.
4ti-0625
Fully skirted 1-component 384
well PCR plates (FrameStar®
384 well plates also compatible)
Plate Support Adapter, PCR 384 (4ti-0618, optional extra) 4ti-0618
Shallow well plates
(e.g. FrameStar®384 Well PCR
Plates)
Plate Support Adapter, Standard (4ti-0615, included)
placed inside
Plate Support Adapter, PCR 96 (4ti-0625, optional extra)
Note: This set-up allows the use of the 4s3™Sealing Frame
(4ti-0612) to keep the seal perfectly aligned.
4ti-0615 + 4ti-0625
Roche 1536 well plates Plate Support Adapter, Roche 1536 (4ti-0616, optional extra)
placed inside
Plate Support Adapter, PCR 96 (4ti-0625, optional extra)
4ti-0616 + 4ti-0625
Deep well plates, fully skirted
SBS standard plates (except 96
well PCR plates)
Plate Support Adapter, Storage 96 and 384
(4ti-0617, optional extra)
4ti-0617
Use of Random Access Heat
Seals with low prole plates
PlateSupportAdapter,RandomAccess,LowProle
(4ti-0613, optional extra)
4ti-0613
Use of Random Access Heat
Seals with standard prole
plates
PlateSupportAdapter,RandomAccess,StandardProle
(4ti-0614, optional extra)
4ti-0614
Use of Random Access Heat
Seals with standard prole
FrameStar®
Break-2-Ways Plates
PlateSupportAdapter,RandomAccess,StandardProle
Break-2-Ways (4ti-0614-1, optional extra)
4ti-0614-1

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4s3™ Semi-Automatic Sheet Heat Sealer
a4S Automatic Heat Sealer, Operation Manual V2.8
4.3 Heat Seal Materials
The 4s3™ is compatible with the widest range of heat sealing materials available on the market. Details of available
materials can be found in the following table, 4titude®literature, or by visiting the 4titude®website www.4ti.co.uk.
Note: 4titude® also offers sample sheets for evaluation purposes. Please contact us at [email protected].
Code Description Dimensions Sheets/Case
4ti-0541 Clear Heat Seal, sheets 125 mm x 78 mm 100
4ti-0575 Clear Weld Heat Seal Mark 2, sheets 125 mm x 78 mm 100
4ti-0581 Clear Heat Seal 3730, sheets on perforated roll 125 mm x 78 mm 1,000
4ti-05481 Clear Heat Seal Plus, sheets 125 mm x 78 mm 100
4ti-0521 Peel Heat Seal, sheets 125 mm x 78 mm 100
4ti-0521/RA-TAB Random Access Peel Heat Seal, With Tabs, sheets 127 mm x 100 mm 100
4ti-0521/RA-8 Random Access Peel Heat Seal 96/8, sheets 127 mm x 100 mm 100
4ti-05231 Universal Peel Heat Seal, sheets 125 mm x 78 mm 100
4ti-0587 DMSO Resistant Peel Heat Seal, sheets 125 mm x 78 mm 100
4ti-0531 Pierce Heat Seal, sheets 125 mm x 78 mm 100
4ti-0531/GR Pierce Heat Seal, sheets, with printed grid reference 125 mm x 78 mm 100
4ti-05381 Pierce Heat Seal Strong, sheets 125 mm x 78 mm 100
4ti-05381/RA Random Access Pierce Heat Seal Strong, sheets 127 mm x 100 mm 100
4ti-0536 Foil Heat Seal, sheets 125 mm x 78 mm 100
4ti-0547 Polystyrene Foil Heat Seal, sheets 127 mm x 100 mm 100
4ti-0591 Thermal Bond Heat Seal, sheets 125 mm x 78 mm 100
4ti-0597 Gas Permeable Heat Seal Mark 2, sheets 125 mm x 78 mm 100
4ti-0597/ST Gas Permeable Heat Seal Mark 2, sheets, sterile 125 mm x 78 mm 10 x 10
4ti-0541/SLIT Gas Permeable Clear Heat Seal, sheets 125 mm x 78 mm 100
4ti-0543 96 INDI Heat Seal, sheets 125 mm x 78 mm 50
4.4 Sealing Parameters
Once you have established that your plate quality is sufcient, you are using the correct plate support adapter
and you have chosen your sealing material, it will be necessary to optimize the sealing parameters of time and
temperature. This can be achieved using empty plates.
In general terms it is sensible to keep one of the parameters constant and vary the other when optimising. For
example, set the sealing time to 2 seconds and gradually increase the temperature , monitor the results until you
are satised with the quality of the resulting seal. You can then further ne tune the quality by adjusting the time,
maintaining your desired temperature, in 0.1 second increments.
The following sealing temperatures and times are for guidance only. Sealing efciency varies depending on the
plate type used. All sealing parameters were estimated using software version 1.07.

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
4.4.1 Sealing Parameters
Note: These values are for guidance only. It is advisable to conduct trials depending on the plate
type being sealed. Add 1 second to the sealing time when the 4s3™Weighted Sealing Platen is used
(4ti-0602)
Code Description Sealing temperature (°C) / Dwell time (sec)
96 well PCR plates* 384 well PCR plates* Vision Plates™**
4ti-0541 Clear Heat Seal 175-185 / 2-3 165-180 / 3 185-200 / 3
4ti-0575 Clear Weld Heat Seal Mark 2 175-180 / 2-3 170-175 / 2-3
4ti-0581 Clear Heat Seal 3730 165-175 / 3 165-175 / 2 175-185 / 2-3
4ti-05481 Clear Heat Seal Plus 175-185 / 2-3 165-180 / 3 185-200 / 3
4ti-0521 Peel Heat Seal 175-185 / 3 170-175 / 2-3
4ti-05231 Universal Peel Heat Seal 175 / 2 175 / 2 180 / 2
4ti-0587 DMSO Resistant Peel Heat Seal 175-185 / 3 170-175 / 2-3
4ti-0531 Pierce Heat Seal 160-175 / 2 160-175 / 2 185-200 / 3
4ti-05381 Pierce Heat Seal Strong 170-180 / 2 170-180 / 2 180-200 / 3
4ti-0536 Foil Heat Seal 165-180 / 2 160-175 / 2-3 185-200 / 3
4ti-0547 Polystyrene Foil Heat Seal 185-200 / 3
4ti-0591 Thermal Bond Heat Seal 170-180 / 2-3 160-170 / 2
4ti-0595 Gas Permeable Heat Seal Mark 2 165-175 / 3 165-175 / 3 175-185 / 3
4ti-0541/SLIT Gas Permeable Clear Heat Seal 175-185 / 2-3 165-180 / 3 185-200 / 3
4ti-0543 96 INDI™ Heat Seal 175 / 3
* These values are for two-component PCR plates. For one-piece polypropylene plates, increase dwell time by one second
** These values can be used for all polystyrene plates
4.4.2 Sealing Parameters Random Access Seals
Note: These values are for guidance only. It is advisable to conduct trials depending on the plate type
being sealed.
Code Description Sealing temperature (°C) / Dwell time (sec)
Plate Support Adapter required for low / standard prole plate
Random Access
Plates
4ti-0613 / 4ti-0614
FrameStar®
Break-A-Way Plates
4ti-0613* / 4ti-0614
FrameStar®
Break-2-Ways Plates
4ti-0613** / 4ti-0614-1
4ti-0521/RA-TAB Random Access Peel Heat Seal,
With Tabs
180 / 3 185 / 4 185 / 4*
4ti-0521/RA-8 Random Access Peel Heat Seal
96/8
n/a 185 / 4 185 / 4*
4ti-05381/RA Random Access Pierce Heat Seal
Strong
185 / 5 185 / 5 185 / 5*
* Applies to FrameStar®Break-A-Way Plates, LowProle with a lot no. >504035. Plates with a lot no. <504035 will be
compatible with adapter 4ti-0613-1 (for details please contact us at 4TI-T[email protected]).
** For FrameStar®Break-2-Ways Plates, LowProle, increase dwell time by one second.

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4s3™ Semi-Automatic Sheet Heat Sealer
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5 Troubleshooting
A list of possible error messages generated by the 4s3™ are summarised within this chapter.
If the solutions given below do not solve the problem, please contact 4titude at [email protected] or their
authorised local distributor
5.1 Error Messages
Display Message Error
Er1 The system cannot reach the home position within a dened time
Er2 The system cannot control the heating element correctly
Er3 The sealer cannot complete the sealing compression within a dened time
Er4 The system cannot lower the elevator within a dened time, following sealing
Er5 Elevator motor is overloaded
Er6 Temperature sensor not functional
Er7 Drawer error
Er8 Low handler sensor error during compression
ErA Drawer motor overloaded
ErB Drawer cannot reach the open or closed position
ErC Drawer does not move or the system does not detect the movement
ErD “close drawer sensor” and “open drawer sensor” error, both the sensors are active at the same time
The errors will remain displayed until the problem is solved. To clear the error press the “CLOSE” (DOWN) button.
If any of the above errors persists switch “OFF” the unit and then switch “ON” again.
If within 10 seconds the problem is not solved the heated platen is automatically turned off to avoid sample
damage.
If the problem is solved within 10 seconds the unit will automatically return to “Run Mode”.
5.2 Cleaning the Heating Platen
If the sealing lm is placed upside down on the plate, the sealing lm will become stuck to the heating platen. To
clean it, proceed as follows:
Note: Do not attempt to clean the unit until it has cooled down to near room temperature!
• Set temperature to “OFF” (please refer to section 3.2)
• Allow unit to cool until heating platen is close to ambient
• If drawer is not open, press the “OPERATE” (UP) button to open
• Switch the unit off at back via the I/O switch
• Disconnect the mains power supply

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4s3™ Semi-Automatic Sheet Heat Sealer
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• To allow better access to heating platen, lay the unit gently onto the side
4s3™ placed onto the side to give easier access to heating platen
• Clean heating platen using a blunt plastic tool, such as a spatula. Gently scrape away the hardened plastic
• Clean any residue left on the heating platen with a damp cloth
• Once clean, return the unit to its upright position
• Ensure nothing is obstructing the drawer, reconnect the mains and reboot the instrument
• The drawer will automatically close
• The system will automatically heat back up to the last set temperature
6 Maintenance
The unit does not require regular maintenance but it should be kept clean
The external casing of the heat sealer can be cleaned with a cloth dipped in water or ethanol (methanol,
formaldehyde can also be used).
The unit should not be immersed in solvents.
Do not use acetone or abrasive cleaners.
No parts are to be autoclaved (except adapters).
In case of radioactive spillages it is recommended to use an appropriate cleaning agent.
For any other maintenance operation not indicated in this manual, please contact 4titude.
Note: Any cleaning procedure must be followed with the unit switched off, the power cable
disconnected and the heated parts at ambient temperature.

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4s3™ Semi-Automatic Sheet Heat Sealer
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7 AppendixA:TechnicalSpecications
Parameter Specication
Mechanical Dimensions (H x D x W) 313 mm x 277mm x 181 mm
Weight 10 kg
Electrical Voltage 00-115/230 ±10% Vac
HT121TS_4B_en_d.doc Page 12 of 35
Frequency 50/60 Hz
Power at 100-115V 240-300 VA
Power at 230V 300 VA
Environmental Temperature range Operative 18°C - 35°C
Storage -20°C – 40°C
Relative humidity 20% - 80% not condensing
Maximum altitude 2000 m
Sound Pressure Level Maximum measured level 60 dbA (below the limits of 85 dbA dened by regulation in force)
Safety Information Pollution degree 2
Overvoltage category II
Devices for use indoors
Setup Parameters Sealing temperature: OFF or from 60°C to 200°C
Step: 1°C
Default: OFF
Sealing time: from 0.0 to 10 seconds
Step: 0.5 second
Default: 3 seconds
Stand by cycle: “no” stand-by or from 1 to 99 minutes
Step: 1 minutes
Default: 60 minutes
Stand by temperature: 60°C, not programmable
HT121TS_4B_en_d.doc Page 13 of 35
Power down temperature: ambient
Warning acoustic (Buzzer): ON/OFF
Default: ON

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4s3™ Semi-Automatic Sheet Heat Sealer
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8 Appendix B: Ordering Information
Code Name/Description Quantity
4ti-0655 4s3™ Semi-Automatic Sheet Heat Sealer
Includes: Power cord, manual, standard plate support adapter (4ti-0615), 12 months warranty
1
4ti-0602 4s3™ Weighted Sealing Platen 1
4ti-0612 4s3™ Sealing Frame
For use with 4s3™ Plate Support Adapter, PCR 96 (4ti-0625)
1
4ti-0613 4s3™ Plate Support Adapter, Random Access, Low Prole
For use of Random Access Seals with low prole plates
1
4ti-0614 4s3™ Plate Support Adapter, Random Access, Standard Prole
For use of Random Access Seals with standard prole plates
1
4ti-0614-1 4s3™ Plate Support Adapter, Random Access, Standard Prole
Break-2-Ways
For use of Random Access Seals with standard prole FrameStar®
Break-2-Ways plates
1
4ti-0615 4s3™ Plate Support Adapter, Standard
For skirted 96 and 384 well plates
1
4ti-0616 4s3™ Plate Support Adapter, Roche 1536
For Roche 1536 well PCR plates
1
4ti-0617 4s3™ Plate Support Adapter, Storage 96 and 384
For 96 well and 384 well storage plates
1
4ti-0618 4s3™ Plate Support Adapter, PCR 384
For 384 well PCR plates
1
4ti-0625 4s3™ Plate Support Adapter, PCR 96
For 96 well PCR plates, includes 4s3™ sealing frame (4ti-0612)
1

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9 Appendix C: Warranty
4titude®warrants that the 4s3™ Semi-Automatic Sheet Heat Selaer (4ti-0655) should be free from defects in
materials and workmanship for a period of 12 months from the date of purchase. The purchase date is determined
by the invoice date from 4titude®to the customer. If the instrument is being incorporated into an automated system
by a third party, the warranty period may be extended by a maximum of 6 months or the date the system is
commissioned, whichever is the shorter. For this automation extension to be valid, 4titude® must be notied of this
requirement along with the details of the integrator at the point of purchase.
Each 4s3™ Heat Sealer is tested and documented by the manufacturer before shipping. 4titude®Ltd‘s Quality
Control System guarantees that the performance of the 4s3™ Heat Sealer you have purchased is within its
specications.
The warranty covers all parts and labour costs associated with a repair of the unit within the rst 12 months. The
need for returning a unit for service must rst be agreed with 4titude®via telephone support. Once it is established
a return is necessary, 4titude®will issue a returns number, details of which must be returned with the unit.
The warranty does not cover defects caused by excessive wear and tear or damage due to shipping, accident,
abuse, misuse, problems with electrical power, or usage not in accordance with product instructions, if other than
original spare parts supplied by the manufacturer have been used, or if other than original 4titude®seal sheets
have been used.
The warranty does not automatically cover shipping charges. Shipping costs (both ways) will be covered by
4titude® where a returns number is issued within 8 weeks of the original delivery date (as conrmed by the invoice
date). Shipping costs after this period will need to be covered by the customer.
Once returned to a 4titude®designated service centre, the unit will be inspected and repaired accordingly and a
report provided to the customer. 4titude®would expect to carry out this work and return the unit within 10 working
days of receiving the unit.
Onsite service or a swap out service (where a loaner instrument is shipped to the customer whilst theirs is
repaired) can be arranged at extra cost. Please contact 4titude®if you are interested in this service.
This standard warranty can be extended to 24 or 36 months respectively.
Extended warranty must be purchased within 4 weeks of the original invoice address.
Code Description
4ti-0655-10 Standard warranty (12 months)
4ti-0655-11 Warranty extension year 2
4ti-0655-12 Warranty extension year 2 and 3
4ti-0655-113 Warranty extension year 3
Please contact 4titude®or your local distributor for pricing details.
The warranty does not cover damage caused to the unit in shipping due to unsuitable or insufcient packing being
used. Wherever possible, the original shipping box should be retained by the customer and used for returning the
unit.