89 North PhotoFluor II User manual

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Rev D
PhotoFluor®II and
PhotoFluor II NIR
Operating Manual
This manual contains important information necessary for the safe
and efficient operation of the PhotoFluor II family of light sources.
Please read the manual in its entirety and heed all safety warnings
before operating the light source.
Follow all safety precautions!
The 89 North®PhotoFluor®II (10999-000-000) and PhotoFluor II
NIR (10998-000-000) light sources are designed and tested in
accordance with ANSI/UL 61010-1, CAN/CSA C22.2 Number 61010-
1, CENELEC EN 61326-1:2006, and FCC Part 15 Subpart B, Class
B.
Prior to use, carefully unpack and inspect all components for any
signs of damage which may have occurred during shipping. If
shipping damage is suspected, notify 89 North or your authorized 89
North distributor immediately.
89 North®
1 Mill St. Unit 285
Burlington, VT 05401
U.S.A.
877-417-8313
[phone]
www.89North.com
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Contents
1Introduction and Intended Use ..........................................4
2Safety ..............................................................................5
3System Overview ..............................................................8
4 Initial Setup....................................................................10
5Adapters and Liquid Light Guides .....................................12
6Neutral Density Control ...................................................13
7Monitoring Operation ......................................................14
7.1 Normal Mode ..................................................................14
7.2 Sleep Mode.....................................................................14
7.3 User Set Mode ................................................................15
8Warning Messages ..........................................................16
9Maintenance ...................................................................22
9.1 Troubleshooting..............................................................23
9.2 Replacing the Fuses ........................................................25
9.3 Disposal of the Lamp.......................................................25
9.4 Limited Guarantee / Warranty..........................................25
9.5 Technical Specification ....................................................26
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Special instructions are emphasized as follows:
NOTE:
This term contains important information
regarding set-up and operation to facilitate
ease of use and obtain effective results.
This term contains critical information regarding
safe handling and use of this system. Device
malfunction or property damage could result if
these instructions are not followed.
This symbol cautions that surfaces may be hot
and could be a burn hazard.
This symbol indicates the location of a
grounding lug.
WARNING:
This term contains critical information by
identifying conditions or practices that may
result in injury or loss of life if these instructions
are not followed.
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1Introduction and Intended Use
The 89 North®PhotoFluor®II and PhotoFluor II NIR are light sources
for fluorescence microscopy. They contains a 200 W metal halide
lamp and associated optics to deliver bright illumination from 340 nm
to 650 nm (PhotoFluor II) or 360 nm to 850 nm (PhotoFluor II NIR).
The illumination is directed to the microscope via a liquid light guide
(LLG). The light guide limits heat delivery to the microscope and
provides flexibility in the placement of the main body of the light
source. The light source supports both 3mm and 5mm core
diameter liquid light guides.
Both the PhotoFluor II and PhotoFluor II NIR use specific light guides
to maximize the output in their intended transmission bands. Using
the incorrect liquid light guide will compromise the performance of
the light engine and prematurely age the light guide.
The metal halide lamp used will operate for greater than 1500 hours
of use. The light source will report the number of hours of use on the
display panel.
Because of its intense brightness, the light source includes an
electronic motor driven neutral density filtering device that allows the
user to attenuate the light that is delivered to the microscope. The
amount of light that is being transmitted through the system is
reported on the display.
Only the lamp and Light Guide supplied by
the manufacturer shall be used. The use of
other products may impair the safe
operation and protections provided by the
equipment.
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2Safety
The PhotoFluor II family of light sources uses a powerful lamp that
produces heat as well as visible, UV light and NIR light (PhotoFluor II
NIR). Proper care must be taken in the setup and operation to
prevent injury.
If the equipment is used in a manner not
specified within this manual the protection
provided by the equipment may be impaired.
Never open or remove the top cover. All
maintenance, including lamp replacement,
must be performed by qualified personnel only.
The lamp contains trace amounts of mercury
and other metals. If the lamp is damaged or
broken, proper handling and disposal are
required for safety.
www.lamprecycle.org
Do not operate the unit near any flammable
materials including flammable gases or liquids.
The light source is designed to sustain a bulb
burst within the light engine enclosure. The
light source may, however, suffer internal
damage as a result. Only qualified personnel
shall inspect the unit for internal damage.
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WARNING:
Inhaling mercury vapor or small particles of
mercury or its compounds can be harmful to
lungs, kidneys, and the nervous system.
Injuries to one’s health can also arise due to
penetration of the skin or resorption via the
gastro-enteric tract.
WARNING:
To prevent risk of explosion:
Replace lamp after 1500 hours of use with
the required bulb only
Do not bump the unit while in use
Do not touch any portion of the bulb except
the ceramic base and power wires as
improper handling may shorten bulb life and
may lead to catastrophic failure of the lamp.
WARNING:
The lamp produces significant amounts of
ultraviolet radiation. Never look directly at the
lamp or at light reflected from the lamp or the
output from the liquid light guide.
WARNING:
Never operate the lamp with the light guide
disconnected from the microscope as ultraviolet
and NIR radiation can be harmful to the
unprotected eye and skin.
WARNING:
LAMP EXPLOSION HAZARD - The lamp
contains toxic gasses under high pressure. In
the event of an explosion, mercury vapor, high
temperature gasses, and very fine glass may
exit the unit.
All persons should leave the surrounding area
at once to prevent mercury inhalation.
The area should be ventilated for 30 minutes.
The area surrounding the light source should be
cleaned using an appropriate absorbent such as
Mercurisorb® or Mercon™ Mercury Spill Kit II or
Mercury Spill Clean-Up Kit.
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WARNING: The lamp produces significant amounts of heat.
Always allow the light source components to
cool to ambient temperature before attempting
any adjustments or replacement of parts.
WARNING: After 1500 hours of operation, the metal halide
lamp must be replaced to ensure suitable
performance and safety. The light source will
alert the user at 1500 hours that the lamp is due
for replacement. At 1600 hours, the light engine
will cease operation as the bulb is no longer safe
to use.
WARNING: Used lamps should not be discarded in the
conventional waste stream. The lamps contain
metals that must be handled as potentially toxic.
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3System Overview
Front Panel Controls:
Rear Panel:
Menu / Enter / Esc (touch)
Sleep / Wake (hold 5 seconds)
▼Shutter Toggle (Normal Mode
Main Screen)
▲▼Option Select (User Set Mode
Screen)
◄►Filter Position (Normal Mode
Main Screen)
Option Toggle (User Set
Mode Screen)
Light Port
Computer Port
Power Switch
Power Plug
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Safety Interlock:
Internal Compartments:
ND Filter
Bulb
Lamp Chamber
Interlock Switch
Filter Wheel Chamber
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4 Initial Setup
System Components
The PhotoFluor II and PhotoFluor II NIR illumination systems are
comprised of:
Light Source
Liquid Light Guide (specific to both the PhotoFluor II and the
PhotoFluor II NIR)
Microscope Adapter (as required)
Electrical Power Cord
USB to Serial adapter
Bulb
Carefully unpack all components, giving particular care to the liquid
light guide. The light guide should never be bent severely or it will
sustain irreversible damage. Also take care not to touch or
contaminate the ends of light guide. If necessary the ends can be
cleaned with isopropyl alcohol and a soft cloth.
Picking a Location
Set the light source on a flat surface in a place that allows for
adequate air ventilation on all sides. Do not position the unit so that
any of the sides (including the back or the top) are up against a wall
or other equipment.
NOTE:
Maintain at least 6” of clearance around all sides
of the light source for adequate ventilation.
Light Guide Connection
Push the wider end of the light guide into the acceptor at the back of
the unit until it snaps in place. Do not force the light guide.
Using an Allen wrench, attach the adapter (as required) to the epi-
illumination port of the microscope. Insert the end of the light guide
and secure in place by turning the red knob firmly to the right.
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Connecting the Power Cord
Insert the power cord receptacle end into the AC receptacle in the
back of the unit. Insert the power cord plug end into a standard AC
outlet. See the technical bulletin in this manual for power
requirements.
Turning the Unit “ON” Using Rear Panel Switch
Activate the main power switch in the back of the unit next to the AC
receptacle. Your unit should then power on the lamp. After three to
six minutes the lamp should be fully warmed up and ready for use.
To avoid damage to the lamp DO NOT turn the
lamp off in the first three minutes while it is
warming up.
NOTE:
Avoid turning off and restarting the bulb during daily use
unless it will not be used for an extended time. Bulb life is
shortened by excessive restarts. For typical daily use it is
best to let it run.
NOTE:
This equipment has passed testing for EMI / RFI
radiation and susceptibility; however, if not installed and
used in accordance with the instructions, interference to
other devices in the near vicinity may occur. If this
equipment does cause harmful interference to other
devices, which can be determined by turning the
equipment off and on, the user is encouraged to try to
correct the interference by one or more of the following
measures.
Reorient or relocate the receiving device.
Increase the separation between the equipment.
Connect the equipment into an outlet on a circuit
different from that to which the other device(s) are
connected.
Consult the manufacturer or field service technician for
help.
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5Adapters and Liquid Light Guides
The PhotoFluor II and PhotoFluor II NIR light sources have adapters
available for a wide arrange lf microscope and imaging systems.
Contact 89 North or an authorized 89 North reseller for more
information.
Two different diameter liquid light guides are available. The
PhotoFluor II and PhotoFluor II NIR each use different LLGs to
maximize their specified transmission range. Use of the wrong LLG
will both comprise the output power of the system and result in
premature light guide aging. Only use the LLG specified for your
version of PhotoFluor II.
PhotoFluor II
PhotoFluor II NIR
3 mm x 2M Liquid
light guide
20001-000-000
20023-000-000
5 mm x 2M Liquid
light guide
20003-000-000
20024-000-000
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Neutral Density Control
The PhotoFluor II and PhotoFluor II NIR, as shipped, have five levels
of internal attenuation for the lamp output. The level of attenuation is
adjusted by either the front panel controls or through optional
external software. An internal electronic drive system moves the
filters into position as well as actuates the light shutter feature.
Position
Transmission
1
100%
2
75%
3
60%
4
40%
5
20%
6
Closed
The ◄ and ► arrows on the keypad step the filters in the Main
Screen (Normal Mode). The ▼ arrow controls the shutter toggle.
NOTE:
Neutral density filters may be replaced with specific high
temperature tolerance excitation filters. Replacing a
neutral density filter with an unapproved glass filter may
result in damage to the unit. Call 89 North for details and
assistance regarding acceptable filters and filter
replacement.
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6Monitoring Operation
Display Modes: Normal Mode, Sleep Mode, and User Set Mode
6.1 Normal Mode
The Normal Mode displays the operational status of the light source.
This is the default display after the 3 minute warm-up screen.
“Normal Mode” layout for the display is shown below:
LAMP HOURS = 835
TRANSMISSION = 100%
SHUTTER = OPEN
6.2 Sleep Mode
Sleep Mode can be activated while the unit is in Normal Mode by
holding the green button for 5 seconds until the unit powers off.
Repeat the sequence to awaken the unit. Re-initialization will happen
as the bulb starts up again so the standard 3 minute warm-up will still
apply.
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6.3 User Set Mode
User Set Mode allows the user to tailor the factory pre-set default
settings to their personal preferences.
The green enter button toggles Normal Mode and User Set Mode
with a single momentary press. The ▲and ▼buttons move the
cursor up and down while the ◄and ► buttons toggle the settings
and increase or decrease values. The green enter button will save
the settings and exit the menu.
The figure below shows the layout for the User Set display.
USER SETTINGS MENU
AUDIBLE ALARM = HI
CONTRAST LEVEL = 8
BACKLIGHT LEVEL = 5
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7Warning Messages
Warning messages display important information about the unit. The
following section describes the protocol for each message. Some
warnings automatically reset, while others require user intervention.
Visual warnings will always be accompanied with audio beeping.
Warning –Power Supply Failure
WARNING!
POWER SUPPLY FAILURE
REFER TO
USER MANUAL
This warning indicates a
power supply failure. The
lamp will be powered down
and remain off. Cycle the
AC power. If the problem
persists, call for technical
help.
Warning –Lamp Temperature
WARNING!
LAMP BOX TEMPERATURE
HIGH, ENSURE PROPER
VENTILATION
This warning signals a
ventilation problem which
could be a warm ambient
environment, obstructed
vents, or a fan failure.
Assure proper ventilation
(clean vents and clearance).
The warning will clear if the
internal temperature of the
unit decreases below the
warning temperature. If the
message persists, call for
technical help.
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Warning –Lamp Temperature Exceeded
WARNING!
LAMP BOX TEMPERATURE
EXCEEDED AND LAMP
HAS BEEN TURNED OFF
This latching warning shuts
down the bulb because of a
ventilation problem which
could be a warm ambient
environment, obstructed
vents, or a fan failure.
Assure proper ventilation
(clean vents and clearance).
Re-power the unit to clear
the message. Call for
technical help if the problem
persists.
Warning –Ballast Temperature
WARNING!
BALLAST BOX TEMP
HIGH, ENSURE PROPER
VENTILATION
This signals a ventilation
problem which could be a
warm ambient environment,
obstructed vents, or a fan
failure. Assure proper
ventilation (clean vents and
clearance). If the message
does not clear, call for
technical help.
Warning –Ballast Temperature Exceeded
WARNING!
BALLAST BOX TEMP
EXCEEDED AND LAMP
HAS BEEN TURNED OFF
This latching warning shuts
down the bulb because of a
ventilation problem which
could be a warm ambient
environment, obstructed
vents, or a fan failure.
Assure proper ventilation
(clean vents and clearance).
Re-power the unit to clear
the message. Call for
technical help if the problem
persists.
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Warning –Switch Failure
SWITCH FAULT
REFER TO
USER MANUAL
This warning is displayed if
a control button becomes
stuck in the “on” position.
Retry switches and buttons
to see if the faulty
connection is freed. Call for
technical help if the problem
persists.
Warning –Cover Open
WARNING!
COVER IS OPEN
BALLAST IS OFF
This warning is displayed
when the cover is not
secured. The ballast and
lamp will be disabled.
Secure the cover with 4
screws and re-power the
unit to clear the message.
Call for technical help if the
message persists
Warning –Thermostat Over-Temperature
FATAL ERROR
THERMOSTAT FAILURE
REFER TO
USER MANUAL
This warning indicates
either the unit has
overheated or has a sensor
failure. Assure proper
ventilation and clean
unrestricted vents. Call for
technical help if the problem
persists.
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Warning –ND Filter Failure
WARNING!
ND MOTOR FAILURE
REFER TO
USER MANUAL
This warning indicates the
filter wheel has failed to
calibrate. Remove the top
cover and check for
obstructions around the
filter wheel. Call for
technical help if the problem
persists.
Warning –Ballast Interface Not Connected / Responding
WARNING!
BALLAST OFF
REFER TO
USER MANUAL
Lamp or ballast did not start
or the lamp is not plugged in
or the lamp has failed. Shut
down and restart. If the
error persists, have qualified
personnel check the
installation of the lamp. If
the lamp is intact and is
properly installed and the
error persists, call for
technical help.
Warning –Ballast Thermistor Outside of Normal Range
WARNING!
BALLAST BOX SENSOR
PROBLEM REFER TO
USER MANUAL
Shut down and restart. Call
for technical help if the
problem persists.
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