Markes International BioVOC-2 User manual

BioVOC-2
QUI-1172
Version 1
October 2019
Instruction for use
Product reference:
BioVOC-2 breath sampler, pk 1 C-BIO02-01
BioVOC-2 breath sampler, pk 10 C-BIO02-10
BioVOC-2 breath sampler, pk 100 C-BIO02-100
Disposable cardboard mouthpieces,
suitable for BioVOC-2, pk 100 C-B100M
www.markes.com

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1. Introduction
BioVOC-2 is a breath sampler that captures a portion of expiratory breath for
transfer to sorbent tube for subsequent analysis using thermal desorption (TD)–gas
chromatography (GC).
2. Components
BioVOC-2 comprises 6 components:
[1] The BioVOC-2 body.
[2] The cap.
[3] The plunger, with plunger tip.
[4] A bung.
[5] A disposable cardboard mouthpiece.
[6] A non-return valve (located within body).
The following items will also be required, but are not supplied with the BioVOC-2:
■Conditioned sorbent tubes.
■Storage caps (C-CF020).
■CapLok™tool (C-CPLOK).

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3. Using the BioVOC-2
[1] Place the disposable cardboard mouthpiece over the nozzle of the BioVOC-2
cap and provide the BioVOC-2 to the subject.
[2] The subject should exhale into the sampler, according to the modalities xed
by the study protocol (e.g. normal exhalation, forced exhalation, breath
holding etc.).
[3] After sample collection, remove the mouthpiece and place the bung into the
tip of the BioVOC-2 body.
Ensure to do this as quickly as possible to minimise loss of sample and
prevent diffusive ingress of air into the BioVOC-2 body.
Step [1] attaching mouthpiece
Step [2] Sample collection

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[4] Attach the plunger to the end of the non-return valve, ensuring the plunger tip
is in position.
[5] Remove the long-term storage caps from the conditioned sorbent tubes using
the CapLok tool.
Step [5] Removing storage caps
Step [4] Inserting plunger

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[6] Remove the bung and attach the sampling end of the sorbent tube to the tip of
the BioVOC-2 body, so that the sampling arrow is pointing away from the
BioVOC-2.
[7] Push the plunger steadily through the length of the BioVOC-2 to displace the
trapped air sample onto the tube. This should take approximately 10 seconds.
[8] Wait 5 seconds and remove the sorbent tube from the
end of the sampler.
Step [7] Transferring sample
Step [6] Inserting tube

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[9] Using the CapLok tool, seal the tube with long-term storage caps to secure
your sample for short-term and long-term storage.
[10] Once the tube is sealed, ush the BioVOC-2 by pulling and pushing the
plunger in and out three times. Return the piston to the end cap ensuring
the non-return valve sits securely within the cap and unscrew the plunger.
[11] If the study protocol requires, you are now ready to take a repeat sample. To
do this, place a new disposable mouthpiece over the nozzle of the BioVOC-2
cap, provide the sampler to the subject, and repeat as before. Please note
that sampling can also be repeated onto the same tube.
[12]
Once your repeat sample tube is sealed, you are ready for analysis.
NOTES Whilst BioVOC-2 is considered disposable, it can be used multiple times
for the same patient during a single sampling sitting – just ensure to
replace the mouthpiece each time.
Step [9] Capping tube

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4. Specications
Part Number Description
C-BIO02-01 BioVOC-2, pk 1
C-BIO02-10 BioVOC-2, pk 10
C-BIO02-100 BioVOC-2, pk 100
C-B100M Cardboard mouthpieces, pk 100
BioVOC-2 volume
■Volume of BioVOC-2 body: 129 mL
Compatible tube types
■Any industry-standard 3½” x ¼“ sorbent tube
5. Contact details
For technical support, please contact your supplier in the
rst instance. If they are unable to resolve your query,
please contact Markes International’s service department:
E: suppor[email protected]om
T: [phone]
W: www.markes.com
For an instructional product video, please visit:
chem.markes.com/BioVOC-2
Scan the code to
watch the video

QUI-1172
L-0153 (081019-1)
CapLok™is a trademark of Markes International.