Abnova Precipitor 32 User manual

Affinity Magnetic Precipitation System
USER MANUAL
Precipitor 32
®

Table of Contents
Check list
Safety Items
Chapter 1 Introduction of Precipitor
Chapter 2 Introduction of LCD
Chapter 3 The picture of wiring at the
back of controller
Chapter 4 I/O Chart
Chapter 5 Troubleshooting guide
Chapter 6 Specication
Chapter 7 Program import/export
Chapter 8 AutoMag software
1
2
3
6
13
13
14
15
16
17

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Check List
Documents
● User manual
● Quality control checklist
Accessories
● USB to serial converter driver disc
● USB to RS-232 transmission line
● RS-232 to USB connection line
● Magnetic base
Consumables
● 4X 96 deep well plates
● 8X 8-channel strips

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Precipitor 32
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Safety Items
Caution!
Notice these tips below for avoiding electric shock or re accidents.
1. Do not touch any plugs and electric switches with wet hands.
2. Turn off and unplug the machine before moving it.
3. Fasten robot before moving and delivering the machine.
4. Put the machine in a room with good ventilation. Keep more than ve centimeters
around the machine.
5. Hold the plug while you are pulling it out of an outlet. Do not pull the electric wire
directly.
6. Do not place electric wires on any subject with high temperature.
7. Do not look steadily at the ultraviolet lamp while it is on.
8. Disconnect the plug from the power source when the machine is not used.
9. Do not mend the machine by yourself. Make contact with our service center or
agencies to deal with problems.
Warning
Please remove the two xing screws inside the internal compartment before turning
on the machine. The two screws were used to hold the magnetic rod set during
shipping, and it has to be REMOVED before turning on the power. This is a very
important step. If not, the instrument will experience malfunction and break.

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Introduction
What is Automatic Platform for Magnetic System?
Preciptor 32 is a robot designed to handle magnetic reagent assays. It is popularly
applied to the extraction of nucleic acids/proteins and the separation of cells.
Users just need to put samples into a pre-lled reagents plate. Then, combining a
96-deep-well plate and a specially-designed strip, the Preciptor 32 can automatically
handle 32 samples simultaneously.
Also, the extraction process of the magnetic system is simplier than that of the spin
column system and still achieves scientic and diagnostic requirements with relatively
quick, easy, and safe operating procedures.
How to perform?
Preciptor 32 uses magnetic rod to transfer magnetic beads from one well to another
and strip to mix solution. By moving the strip up and down to mix solution well,
you can get clean nucleic acids after cells lysis, nucleic acids absorption, wash and
elution.
Operation interface is simple.
There are five parts in operation interface: Select Program Screen, Perform Temp
Screen, Perform UV Lamp Screen, Perform Program Screen and Control Panel. The
control panel includes 7 buttons and uses LED light to indicate operator.
Heating system
The heating system can provide reagents with the best working temperatures.
All temperatures are programmed. In Perform Program Screen, “PRESET” is
programming temperature and “PRESENT” is real temperature on stainless steel board.
The heating system is connected with the main power system. Switch off both of the
main power and heating switch when you turn your machine off. If you are not going
to start this function next time, please remember to turn it off.
There is a light on the heating switch and it will be lit up as the heating is functioning.
Meanwhile, the temperature of stainless steel board will raise and please keep clear of
the machine.

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Precipitor 32
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Appearance of the Machine
Front view Back view
Control Panel
Switches and Electrical Inlet (voltage: 100~240V, fuse: 5A/250V)
(A) Heating switch
(B) Main power switch
(C) USB port
(D) Electrical inlet
Internal view
(A) Magnetic rod frame
(B) Strip rack frame
(C) 96 deep well plate rack
(D) 8-channel strip
(E) 96 deep well plate
D
A
B
C
A
B
D
E
C

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: LED light-up indicates users to press the button. Start bottom LED
is green, and others are red.
: to initiate the program.
: to terminate the program. It shows “ ” at status row at the rst
time you press the button. Press the button again to conrm to stop
the program. Then the robot will be back to the initiated site.
: Press the button to turn buzzer-beater off.
: It stops temporarily when you press the button. Press the button
again to continue program, and the time will be recalculated.
: 1. Press the button to select the previous program or a number.
2. From .
: Conrm the program or the number you select.
Move down: Press the button to select the next program or a number.

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Precipitor 32
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Introduction of LCD
I. Time Setting
Turn on the machine after pressing the button . After 3 seconds, the screen
will automatically show time setting as below.
Time Setting Screen:
Press the buttons or to select the key-in position. Then, press the
button to confirm it. The way to select numbers is to press the buttons
or , and change the digits from 0 to 9. Finally, press the button to
conrm the number you select. The years are started from A.D.. Insert the last two
digits. For example, insert 08 for 2008 A.D. on the screen. It’s 24 hour system.
Please restart the machine after setting the date and the time.
II. Opening Screen
These words will show up on the screen in ve seconds after turning on the power
system of the machine.
Welcome to Precipitor
afnity magnetic precipitation system

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III. Select Program Screen
: The name of the program is no more than 15 characters.
: It display the last program number you used. You can pre-load 100 programs,
code number from 00 to 99.
: Insert the number of the program to select the program directly.
The way to insert digits is to press the button or . Select the digits
from 0 to 9.
Then press the button to conrm it.
TEMP :Press the button or to select the TEMP, then press the button
to enter the “Perform Temp Screen”.
: Press the button or to select the UV Lamp, then press the
button to enter the “Perform UV Lamp Screen”.
IV. Perform Temp Screen
PRESENT : Real working temperature. (℃)
PRESET : Set temperature of bottom stainless plate, RT~65. (℃)
PROGRAM : Return to the “Select Program Screen” after turning off Heater.

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Introduction of TEMP
1. Press the button or to select key-in position and return to “Select
Program Screen”. Then press the button to conrm it.
2. PRESET setting: Press the button or to change the digits from
RT to 70, then press the button to conrm it.
3. Heater ON/OFF: Press the button to turn on or the button to turn
off heater.
V. Perform UV Lamp Screen
: Set working time of UV Lamp. ( hh : mm )
: Start time of UV Lamp ( hh : mm : ss )
: End time of UV Lamp ( hh : mm : ss )
: The present status of UV Lamp (ON or OFF)
: Return to the “ ” after turning off UV Lamp.
: It shows current time in the right lower corner. Time is 24-hour format

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1. Press the button or to select key-in position and return to “
”. Then press the button to conrm it.
2. Time setting: Press the button or to change the digits from 0 to 9,
then press the button to conrm it.
3. UV Lamp ON/OFF: Press the button to turn on or the button to turn
off UV Lamp.
VI. Perform Program Screen
PROGRAM : It shows the name of program.
PRESENT(℃) : Real working temperature. It shows “NC”, when there is no connection
with thermal couple.
PRESET(℃) : Preset working temperature. It shows “NA”, when there is no preset
input.
STATUS : It shows the machine being what kind of status.
END : The program is nished.
RUNNING : The program is progressing.
PAUSE : To make a short stop.
HOME : Go to the initial site.
STOP? : Do you want to stop program?
JOG : To move robot slowly.
LIMIT : The site of robot is out off the limited range.
SENSOR : It is fault to detect the initial site.
TEMP MAX : Real temperature is higher than over-temperature protection.
(80℃)
TEMP ALM : It can not be reached preset temperature in a reasonable
period. (30min)
: (1) If it shows LIMIT or SENSOR, it means the machine is out of order. And
buzzer will keep on ringing. Press the button to stop the buzzer. Turn

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Precipitor 32
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the power system off, and notify our service center to deal with problem.
(2) If it shows TEMP MAX or TEMP ALM, it means heating system is out of order. And
buzzer will keep on ringing. Press the button to stop the buzzer. Turn
heating switch off, and notify our service center to deal with problem.
VOLUME : The buffer volume in the well (μl)
MIXING : The working time of strip(minute)
COLLECT : The working time of magnetic rod(second)
VAPOR : The time for waiting reaction or alcohol vaporization (mm:ss)
START : Start time of program(hh:mm:ss)
END : End time of program(hh:mm:ss)
: It shows current time in the right upper corner. Time is 24-hour format.
VII. Introduction of control panel
1. Press the buttons of or to select the program. Please refer to the
picture below.
Continue pressing the button . It will change to the next program.
2. Input the number of the program at SEARCH position, and it will show the
program directly.

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1. After selecting the program, press the button . And it will show message
in the right lower corner as and . Please press the buttons of
or to conrm the program. Please refer to the picture as below:
Press the button to run the selection program, and then go to “
”. Press the button to return to “ ”.
2. After entering “ ”, press the button to execute the
program.
: Conrm two things below before you start the program.
(1) Did you put 96-deep well plate toward outside by bevel and push it to the bottom
of plate rack?
(2) Did you put 8-channel strip to the bottom of strip rack?

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1. If you press the button in the process of executing program, the
execution will be paused immediately. The robot will stop working and move
back to the top. Meanwhile, other buttons will be unworkable.
: In step, the robot can not be stopped immediately. It will pause
after nishing the collect action. And the magnetic rod and strip will move
back to the top together.
2. Press the button again will continue running the program.
: If you press the button in this stage, the program will be stopped
and nished. The robot will go back to the initial site.
1. Press the button in the process of executing program, the execution will
be stopped immediately. The robot will stop working immediately and it shows
“ ” at status row.
Press the button again to conrm terminating the program and the robot
will go back to the initial site. If you don’t want to terminate the program, press
the button to continue running the program.
: In step, the robot can not be stopped immediately. It will stop after
nishing the collect action. And the magnetic rod and strip will move back to the
top together.
2. the same as emergency button.
3. Another function of button is to move the robot back to the initial site.

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The picture of wiring at the back of controller
outlet connecting to stepping motor
outlet connecting to DC 24V
outlet (X, Y, Z) connecting to 3-axis zero sensor
outlet (D) connecting to emergency door
◎ It means variable electric resistance.
Green 2PIN outlet (J14) connecting to thermocouple
: 9PIN outlet is the usage of transmitting program.
P2 : 26PIN outlet connecting to UV lamp, heater and cooler.
: tune the contrast of LCD
I/O Chart
Input 6 points Output 4 points
X01 X-axis zero sensor Y01 UV lamp
X02 Strip zero sensor Y02 Buzzer
X03 Rod zero sensor Y03 Cool
X04 Safety door sensor Y04 Heat
X05 Reserve
X06 Reserve

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Precipitor 32
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Troubleshooting guide
Problems
It displays nothing on the screen.
Noises from the machine.
The action of the magnetic rod
frame doesn’t work normally.
It doesn’t move down or move up.
The function of heating is abnormal.
The temperature doesn’t increase.
Stain on the magnetic rod.
The magnetic rod comes off the
magnetic rod frame.
Suggestions
Did you turn on the power system already?
Did the plug connect with the power system?
Measure the voltage of outlet.
Check the strip to see if it has been pushed
into the bottom of strip rack.
Check the plate to see if it has been pushed
into the bottom of plate rack.
Contact service center: 886-2-87511888.
Please ma ke su re the t wo sc rews are
removed before turning on the power.
Press the button “Stop” to terminate the
program. Then, press the button “Stop”
again to let the robot move back to the
initial site.
Run DEMO program without 96-deep well
plate. Check the magnetic rod frame to see
if it still is abnormal.
Contact service center: 886-2-87511888.
Check the heating switch to see if it has
been turned on.
Contact service center: 886-2-87511888.
Be sure to put a clean strip on the strip rack
whenever you operate it. Avoid to be stained
by the remaining reagents in the 96-deep
well plate.
Use clean and moisten cotton cloth or wet
tissues to wipe it out carefully. Do not use
any solvent or cleanser to clean it.
Contact service center: 886-2-87511888.

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Specication
Product Name Preciptor® 32
Width(cm) 38
Height(cm) 31
Length(cm) 31
Weight(Kg) 19
Power (V) 100-240
Current (A) max. 2
Operating temperature (℃) RT-65
Humidity Range (%) <80
Processing Volume (μl) 100-1000 per well

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Precipitor 32
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Progarm import / export
1.1 AutoMag.rar le
1.2 USB to serial converter driver disc
1.3 USB to RS-232 transmission line
1.4 RS-232 to USB connection line
2.1 Copy AutoMag.rar to your computer and extract it.
2.2 If you don’t have any software for extraction, please contact your dealer to obtain
AutoMag le.
2.3 You can run AutoMag software by executing the AutoMag in le.
2.4 Key in password to start the software. The original password is “0”. You could
rebuild another set of new password, but no more than 6 Arabic numerals.
3.1 RS-232 transmission line can be connected to the RS232 port of PC.
3.2 For portable computer without RS-232 port, you should connect RS232
transmission line to USB connection line then connect to USB port. Please install
USB to serial converter driver.
If your computer is using the latest operating system, you can download new USB
to Serial converter driver form www.prolic.com.tw.
: Before using the AutoMag program, change the communication port firstly.
The used USB port can be checked in control/system/device manage/port.

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AutoMag software
key in your user name
and password
(6 digits at the most)
change your password
conrm your password
Protocol Edit window:

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Precipitor 32
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: New- open a new le
Open- open an old le
Save- Save a le
End- close the le
: Cut
Copy
Paste
Move up
Move down
Insert
Delete
:
Choose a COM port. There 16 COM ports.
COM1 is a preset port.
Reset a new COM port. The program can
record it so that you won’t have to set it
again when using it.
: The used USB port can be checked in control/system/device manage/port.