Luminex Guava easyCyte Operating manual

Quick Reference Card
Guava® easyCyte™ Maintenance Guide
For the Guava® easyCyte™ and easyCyte™ HT systems
For Research Use Only. Not for use in diagnostic procedures.
Use this quick start guide when cleaning, running Guava easyCheck,
and replacing the flow cell for your Guava easyCyte or easyCyte HT
System. For detailed instructions on using the system, refer to the
appropriate Guava easyCyte System User’s Guide.
Running Guava® Clean
Run Guava® Clean at the start and end of the day, between assays if
needed, and to prime the fluid system if you suspect air in the fluid
lines. If running lysed whole blood, run two Guava Clean cycles.
Guava® easyCyte™ Systems
1. Click Cleaning from the main menu.
2. Click Start Cleaning.
3. Place a tube containing 10% bleach in Guava® ICF and a tube of
DI water in the sample holder.
4. Load the tube of DI water and click OK.
5. When prompted, load the tube of bleach in ICF and click OK.
6. When prompted, load a tube of fresh water and click OK.
Guava® easyCyte™ HT Systems
1. Click Cleaning from the main menu.
2. Click Start Clean.
3. Load the following tubes:
• Seven tubes filled with DI water in positions w1, w2, w3, w4,
w5, w6, and tube position 1
• 100 μL of 100% bleach in tube position 2
• Two tubes of Guava® ICF in tube positions 3 and 4
• Tube of DI water in any tube position 5–9 for capillary shut-
down
4. Click to select the capillary shutdown position on the plate
map, then click OK.
5. If you are shutting down the instrument, click Capillary Shut-
down from the Guava Clean screen. Select a tube from posi-
tions 5 through 9 that contains of tube of DI water and click
OK.
Running easyCheck™
Prepare a 1:20 dilution of Guava® easyCheck™ beads. Refer to the
Guava easyCheck Kit User’s Guide for information. Refer to the
appropriate Guava easyCyte™ System User’s Guide for information
on easyCheck results.
Guava® easyCyte™ Systems
1. Click easyCheck from the main menu.
2. Enter the Bead Lot #, Bead Expiration date, and Expected Parti-
cles/mL.
3. Mix the easyCheck™ bead sample and load it on the instru-
ment. Click Run 1st Replicate.
4. Remove the sample, mix it, then reload it. Click Run 2nd Repli-
cate.
5. Remove the sample, mix it again, then reload it. Click Run 3rd
Replicate.
Guava® easyCyte™ HT Systems
1. Click easyCheck from the main menu.
2. Enter the Bead Lot #, Bead Expiration date, and Expected Parti-
cles/mL.
3. Click Start.
4. Load the following tubes:
• DI water in positions w2, w4, and w5
• Empty tubes in positions w3 and w6
• 100 μL of 100% bleach in position w1
• easyCheck™ bead sample in any tube or well position
5. Click to select the tube location containing the beads, then click
OK.

Replacing the Flow Cell
When replacing the flow cell, handle it with care. The capillary is
fragile; avoid touching it unnecessarily. Do not force the flow cell
into the receptacle.
1. Remove the metal plate from the top of the instrument.
2. Remove the minstac tubing from the clamp and disconnect it
from the instrument.
3. Remove the flow cell by grasping it at the top with your fingers
and pulling straight up. Do not pull up on the tubing. You can
also use the flow cell removal tool to remove the flow cell.
• Grasp top of the flow cell with the removal tool by placing
the clamps into the cutouts at the top of the flow cell, as
shown.
• Pull the tool straight up to remove the flow cell.
4. Install the new flow cell by correctly positioning it above the
instrument and carefully lowering it into the receptacle. It fits
one way only—note the beveled edge.
Avoid bumping the glass capillary against the instrument or
sides of the receptacle.
Push down firmly on the top of the flow cell on either side of the
tubing using your fingers until the flow cell clicks into place. Do
not press down on the tubing.
5. Connect the tubing to the instrument, then insert the tubing
into the clamp.
6. Run Guava® Clean to prime the instrument, followed by easy-
Check™.
Technical Support
Telephone: [phone]
North America Toll Free: 1-877-785-2323
International Toll Free: + 800-2939-4959
Email: [email protected]om
www.luminexcorp.com
Guava is a trademark of Luminex Corporation, registered in the U.S. and other countries.
easyCheck and easyCyte are trademarks of Luminex Corporation.
Part No. 0110-8490 Rev D, July 2019, Printed in the USA.
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