Accu-Scope 3025 Series User manual

Figure 1

3025 Series Turret Phase Condenser
(Bottom View)
Figure 2
Centering Telescope
Figure 3
Rotating
Annuli Wheel
Rotating
Annuli Wheel
Rotating Turret Wheel

3025 SERIES PHASE CONTRAST SET-UP INSTRUCTIONS
1. Place the microscope in front of you and plug in your light source.
2. Mount the objectives clockwise (lowest magnification to highest magnification) onto the
microscope nosepiece.
3. Install the turret phase condenser (Fig. 2) into the condenser holder located under the
stage and tighten into place.
4. Position the turret phase condenser so that the “BF” on the rotatable annuli selector wheel
is facing you. This places the condenser iris diaphragm in the light path. Rack up the
condenser to the top and open the diaphragm.
5. Place a specimen slide on the stage and focus on it using the 10X objective.
6. Shift the slide to an area where the specimen is no longer visible; however, do not change
the focus.
7. Rotate the phase turret wheel counter-clockwise until the “10” figure clicks into position.
Note: There are two rotating wheels located at the front of the phase condenser. They are
used to center the annuli in the condenser or phase rings.
8. Remove one of the eyepieces of the microscope and replace it with the phase telescope
(Fig. 3). Note: The upper collar of the phase telescope can be moved in or out allowing
you to bring the phase rings into focus.
9. Looking through the phase telescope you will see a dark field of view with two different
rings ( a & b), one bright (white) and the other black.
10. While viewing the rings through the phase telescope, adjust the two rotating wheels from
the turret phase condenser until the narrower white ring is superimposed equidistant
within the black ring (c).
11. Remove the phase telescope from the microscope and replace the eyepiece.
12. Bring the specimen back into the field of view and refocus the image until it is sharp. The
image will be detailed and exhibit fine structural information.
NOTE: You must re-center the phase rings every time you change magnification by using a
different objective. The turret wheel must be rotated so its number corresponds with the
objective being used. To return to brightfield viewing, rotate the turret wheel until it is at the
“BF” position.

TROUBLESHOOTING GUIDE
PHASE CONTRAST MICROSCOPY
PROBLEM
CAUSE
CORRECTIVE MEASURE
Poor phase
contrast
image
is obtained
The condenser phase
annular
diaphragm image and the objective
phase plate do not match.
Adjust the phase
annular
diaphragm so that it matches with
the objective phase plate.
The condenser phase annular
diaphragm and the objective phase
code do not match.
Insert the phase
an
nular
diaphragm with the same phase
code as the objective into the
optical path.
The field diaphragm image is not
focused on the specimen surface.
Move the condenser up or down to
focus the image on the specimen
surface.
The phase difference of the
specimen is too large.
Prepare the specimen using a
different sealant and specimen
thickness
The type of pha
se
objective does
not match the phase difference of
the specimen.
Select a phase objective suitable
for the specimen.