Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity Micro
Degasser
User Manual

1260 Infinity Micro Degasser User Manual
Notices
© Agilent Technologies, Inc. 2006,
2007-2008, 2010
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
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Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G1379-90013
Edition
06/10
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

1260 Infinity Micro Degasser User Manual 3
Contents
Contents
1 Introduction 5
Introduction to the Micro Vacuum Degasser 6
2 Site Requirements and Specifications 7
Site Requirements 8
Physical Specifications 11
Performance Specifications 12
3 Installing the Micro Vacuum Degasser 13
Unpacking the Micro Vacuum Degasser 14
Optimizing the Stack Configuration 16
Installing the Micro Vacuum Degasser 19
Flow Connections to the Micro Vacuum Degasser 21
Operational Hints for the Micro Vacuum Degasser 24
Transporting the Micro Vacuum Degasser 27
4 Using the Micro Vacuum Degasser 29
When to Use a Micro Vacuum Degasser? 30
Solvent Information 32
5 Optimizing Performance 35
Increasing the Degasser Performance and Degassing Level 36
6 Troubleshooting and Diagnostics 37
Overview of the Degasser's Indicators 38
Status Indicators 39
Hardware Symptoms 41
7 Maintenance 45
Introduction to Maintenance and Repair 46

41260 Infinity Micro Degasser User Manual
Contents
8 Parts and Materials for Maintenance 57
Cover Parts cover 58
Sheet Metal Kit 59
Power and Status Light Pipes 60
Accessory Kit 61
9 Cable overview 63
Overview 64
Remote Cable 66
10 Appendix 69
General Safety Information 70
The Waste Electrical and Electronic Equipment "WEEE) Directive
(2002/96/EC) 73
Radio Interference 74
Sound Emission 75
Agilent Technologies on Internet 76

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1260 Infinity Micro Degasser User Manual
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1
Introduction
Introduction to the Micro Vacuum Degasser 6

61260 Infinity Micro Degasser User Manual
1Introduction
Introduction to the Micro Vacuum Degasser
Introduction to the Micro Vacuum Degasser
The Agilent 1260 Infinity micro vacuum degasser, model G1379B, comprises a
4-channel vacuum container, including 4 micro structured membranes, and a
vacuum pump. When the micro vacuum degasser is switched on, the control
circuit turns on the vacuum pump, which is operated with constant speed and
voltage generates a partial vacuum in the vacuum container. The pressure is
measured by a pressure sensor. The micro vacuum degasser maintains the
partial vacuum with the help of a controlled leak in the degasser’s
proportional valve and by varying size of the controlled leak within the
proportional valve, depending on the signal from the pressure sensor.
The LC pump draws the solvents from their bottles through the special plastic
membranes of the vacuum container. As the solvents pass through the vacuum
chambers any dissolved gas in the solvents permeates through the membranes
into the vacuum container. The solvents will be almost completely degassed
when leaving the outlets of the micro vacuum degasser.
Figure 1 Overview (only one of the four solvent channels is shown)
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1260 Infinity Micro Degasser User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 8
Physical Specifications 11
Performance Specifications 12

81260 Infinity Micro Degasser User Manual
2Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 1 on page 11. Consequently there is no
voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔Always unplug the power cable before opening the cover.
➔Do not connect the power cable to the instrument while the covers are removed.

1260 Infinity Micro Degasser User Manual 9
Site Requirements and Specifications 2
Site Requirements
Power Cords
Different power cords are offered as options with the module. The female end
of all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
CAUTION Unaccessable power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.
WARNING Absence of ground connection or use of unspecified power cord
The absence of ground connection or the use of unspecified power cord can lead to
electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
➔Never use a power cord other than the Agilent Technologies power cord designed
for your region.
WARNING Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

10 1260 Infinity Micro Degasser User Manual
2Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 11) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench should carry an Agilent system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
WARNING Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage of
electronic equipment.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
CAUTION Condensation within the module
Condensation will damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

1260 Infinity Micro Degasser User Manual 11
Site Requirements and Specifications 2
Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 7 kg (16 lbs)
Dimensions (height ×
width × depth)
345 x 435 x 80 mm (13.5 x 17 x 3.1 inches)
Line voltage 100 – 240 VAC, ± 10% Wide-ranging capability
Line frequency 50 or 60 Hz, ± 5%
Power consumption 30 VA / 30 W / 102 BTU Maximum
Ambient operating
temperature
0 – 45 °C (32 – 113 °F)
1
1This temperature range represents the technical specifications for this instrument. The mentioned
temperatures may not be suitable for all applications and all types of solvents.
Ambient non-operating
temperature
-40–70 °C (-4–158 °F)
Humidity < 95%, at 25–40 °C (77–104 °F) Non-condensing
Operating Altitude Up to 2000 m (6562 ft)
Non-operating altitude Up to 4600 m (15091 ft) For storing the module
Safety standards: IEC, CSA,
UL
Installation Category II, Pollution Degree 2 For indoor use only.

12 1260 Infinity Micro Degasser User Manual
2Site Requirements and Specifications
Performance Specifications
Performance Specifications
Table 2 Performance Specifications Agilent 1260 Infinity Vacuum Degasser
Type Specification
Maximum flow rate 0 - 5 ml/min per channel
Number of channels 4
Internal volume per channel Typically 1 ml per channel
Materials in contact with solvent PTFE, FEP,PEEK
pH range 1 – 14
RS-232 output For diagnostic purposes

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1260 Infinity Micro Degasser User Manual
Agilent Technologies
3
Installing the Micro Vacuum Degasser
Unpacking the Micro Vacuum Degasser 14
Accessory Kit Contents 15
Optimizing the Stack Configuration 16
Installing the Micro Vacuum Degasser 19
Flow Connections to the Micro Vacuum Degasser 21
Operational Hints for the Micro Vacuum Degasser 24
General Priming Instructions 24
Priming the Degasser 25
Priming with a Syringe (only recommended if priming with the pump
fails) 26
Transporting the Micro Vacuum Degasser 27

14 1260 Infinity Micro Degasser User Manual
3Installing the Micro Vacuum Degasser
Unpacking the Micro Vacuum Degasser
Unpacking the Micro Vacuum Degasser
Damaged Packaging
Upon receipt of your micro vacuum degasser, inspect the shipping containers
for any signs of damage. If the containers or cushioning material are damaged,
save them until the contents have been checked for completeness and the
micro vacuum degasser has been mechanically and electrically checked. If the
shipping container or cushioning material is damaged, notify the carrier and
save the shipping material for the carriers inspection.
Delivery Checklist
Ensure all parts and materials have been delivered with the micro vacuum
degasser. The delivery checklist is shown below. To aid in parts identification,
please see “Parts and Materials for Maintenance” on page 57. Please report
missing or damaged parts to your local Agilent Technologies Sales and Service
Office.
Table 3 Vacuum Degasser Delivery Checklist
Description Quantity
Vacuum Degasser 1
Power Cable 1
Service Manual 1
Accessory Kit (“Accessory
Kit Contents” on page 15)
1

1260 Infinity Micro Degasser User Manual 15
Installing the Micro Vacuum Degasser 3
Unpacking the Micro Vacuum Degasser
Accessory Kit Contents
Item p/n Description
1 G1379-68706 Connecting tubing (to connect to channels in series for increased
performance) 2x
2 G1322-67300 Kit of 4 solvent tubes for connection degasser to MCGV (Quaternary
Pump) including labels
3 5062-2463 Tubing Flex 5 m
4 0100-1710 Mounting Tool for Tubing Connections

16 1260 Infinity Micro Degasser User Manual
3Installing the Micro Vacuum Degasser
Optimizing the Stack Configuration
Optimizing the Stack Configuration
If your micro vacuum degasser is part of a system, you can ensure optimum
performance by limiting the configuration of the system stack to the following
configuration. This configuration optimizes the system flow path, ensuring
minimum delay volume (from point of solvent mixing to head of column) and
dead volume (from point of injection to detector outlet).

1260 Infinity Micro Degasser User Manual 17
Installing the Micro Vacuum Degasser 3
Optimizing the Stack Configuration
Figure 2 Recommended Stack Configuration (Front View)
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18 1260 Infinity Micro Degasser User Manual
3Installing the Micro Vacuum Degasser
Optimizing the Stack Configuration
Figure 3 Recommended Stack Configuration (Rear View) 68EdlZgA6CidA88]ZbHiVi^dcdgdi]ZgXdcigdaa^c\hd[ilVgZGZbdiZXVWaZ86C7jhXVWaZid>chiVciE^adiXdbbjc^XVi^dc6cVad\h^\cVaidgZXdgYZg86C7jhXVWaZ[dg^ciZg"bdYjaZ

1260 Infinity Micro Degasser User Manual 19
Installing the Micro Vacuum Degasser 3
Installing the Micro Vacuum Degasser
Installing the Micro Vacuum Degasser
1Place the micro vacuum degasser on the bench.
2Ensure the power switch on the front of the micro vacuum degasser is OFF
(switch stands out).
3Connect the remote cable to the power connector at the rear of the micro
vacuum degasser.
4Option: Connect the interface cable to the micro vacuum degasser. The
remote cable is a one way connection to send a not-ready signal from the
Parts required Description
Power cord
Interface cable as ordered, see “Overview” on page 64
Hardware required Micro degasser
Preparations Locate bench space
Provide power connections
Unpack the vacuum degasser module
WARNING Abnormal conditions
In case of abnormal conditions during operation, the instrument must be
disconnected from line.
➔To disconnect the instrument from line, unplug the power cord.
CAUTION "Defective on arrival" problems
If there are signs of damage, please do not attempt to install the module. Inspection by
Agilent is required to evaluate if the instrument is in good condition or damaged.
➔Notify your Agilent sales and service office about the damage.
➔An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.

20 1260 Infinity Micro Degasser User Manual
3Installing the Micro Vacuum Degasser
Installing the Micro Vacuum Degasser
degasser to the other modules to shut down the whole system after an error
condition of the degasser.
Figure 4 Rear of the Micro Vacuum Degasser
Figure 5 Front of the Micro Vacuum Degasser
5Press in the power switch to turn ON the micro vacuum degasser.
NOTE In an Agilent 1260 Infinity stack, the individual modules are connected through a CAN
cable. The Agilent 1260 Infinity micro vacuum degasser is an exception. The micro vacuum
degasser can be connected via the APG remote cable to the other modules of the stack. An
Agilent 1260 Infinity Instant Pilot can be connected to the CAN bus at any of the modules in
the system except for the degasser. The control software can be connected to the system
through one GPIB or LAN cable (via LAN-Card) at any of the modules (except for the
degasser), preferably at the detector.
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NOTE The power switch stays pressed in and a green indicator lamp in the power switch is ON
when the micro vacuum degasser is turned ON. When the line power switch stands out and
the green light is OFF, the micro vacuum degasser is turned OFF.