Allsheng Auto-Pure Series User manual

Operation Manual
V3.0
Auto-Pure Series
Nucleic Acid Purification
System
Hangzhou Allsheng Instruments Co., Ltd.

Forewords
Thank you for purchasing Auto-Pure series Nucleic Acid Purification System.
In order to use the instrument properly, please read carefully this manual
before operating and keep it for future reference.
Opening Check
Please check the instrument and Appendix with the packing list when you
first open the package. If you find anything missing or incorrect, please
contact the distributor.
HANGZHOU ALLSHENG INSTRUMENTS CO., LTD.
Address: Building 1&2, Zheheng Science Park, Zhuantang Town, Xihu
District,Hangzhou, Zhejiang 310024, China
Telephone: [phone]
E-mail: [email protected]
Website: www.allsheng.com
File No.: AS153
Version: 3.0, Jun. 2019

Safety Warnings and Guidelines
1Warning
Please read this Manual carefully before operation.
2Safety Tips
The operation, maintenance and repair of the Instrument should comply
with the basic guidelines and cautions as below. Improper use of the
instrument may cause damage to the system, inaccurate results, or
potentially nullify warranties.
Operation without reading the manual may cause damage or
even electrical shock.
Indoor use only
Read the Manual carefully before operation, only qualified and
The operator should not open or repair the Instrument without
Vendor’s authorization, if not, there might be cause potential
damages or injuries and affect the warranty.

The Instrument should be placed in a position with low humidity,
less dust, and keep it away from water, sunshine and strong light
source. Make sure of adequate ventilation, no corrosive gases, no
strong magnetic interference and to avoid any heat sources.
Before connecting to power, make sure the voltage used is same
as the instrument required, and the maximum rated load should
be sufficient for the instrument.
Please replace the power cord with same specs if the power cord
is damaged. Please make sure there’s nothing covered the power
cord and keep it away from crowds when in use.
Power off the instrument after operation and please disconnect
the plug if long time no use of the instrument and cover it with
something to prevent from dust.
Under the following circumstances, please disconnect the power
immediately and contact with your distributor.
Liquids into the Instrument;
Drenched by rain or water
Abnormal operation especially like abnormal sound or smell
During operation, the surface temperature of heating block inside
operation window could be very high. To avoid possible scald or
boiling of the liquid, do not touch the metal part when operating.

3 The maintenance of Instrument
The inner side of drawer should be cleaned periodically by the cloth with
alcohol.
If there are any stains on the Instrument, clean them with cleansing
cream.
4 The limiting condition of transportation and storage environment
Ambient temperature range: 10℃～35℃
Relative humidity : ≤70%
Atmosphere range: 500~1060hpa
No corrosive gas and a well-ventilated room.

Contents
Chapter 1 Brief Introduction...............................................................................1
Chapter 2 Specification....................................................................................2
1. Normal operating conditions..........................................................................2
2. Basic parameter & Performance.................................................................... 2
3. Overall Dimension...........................................................................................3
Chapter 3 Product Introduction......................................................................... 4
1. Structure......................................................................................................... 4
2. Operation panel.............................................................................................. 6
Chapter 4 Operation........................................................................................... 7
1. Preparation..................................................................................................... 7
2. Connect the power......................................................................................... 8
3. Install the reagent kit......................................................................................8
4. Install the magnetic rod cover......................................................................10
5. Remove magnetic rod cover.........................................................................11
6. Operation...................................................................................................... 12
Chapter 5 Troubleshooting............................................................................... 29
Chapter 6 Spare Parts List.................................................................................31
Chapter 7 Abbreviation and Symbols...............................................................32

Auto-Pure series Operation Manual Chapter 1 Brief Introduction
─1─
Chapter 1 Brief Introduction
Auto-Pure series Nucleic Acid Purification System use the magnetic rod to
adsorb, transfer and release magnetic particles to transfer the operating
sample and in this way to purify DNA/RNA, protein and cell etc. The
Purification system able to handle 1-20,1~24,1~32 or 1-48 samples
simultaneously with special reagent kit or 96 well reaction plate, and when
using the different reagent, would be able to extract DNA/RNA from animal
or plant tissue, blood and body fluid etc.
Features
Friendly interface with easy operation
Touch screen with 3 shortcut key or mouse operation
Heating function in Lysis and elution
UV steriliza tion
Quiet operation without vibration
One stop operation to avoid contamination
Rapid extraction:10~60 minutes/time
Premium magnetic particles leads to high yield
Extendable Ethernet remote control and WiFi
Extendable APP software for mobile phone and PAD w/android system

Auto-Pure series Operation Manual Chapter 2 Specification
─2─
Chapter 2 Specification
1. Normal operating conditions
Environmental Temperature: 10

C～35

C
Relative Humidity ≤70%
Input: AC 100～240V，50Hz/60Hz
2. Basic parameter & Performance
Table 1 Basic parameter & Performance
Model
Parameter
Auto-Pure
32A
Auto-Pure
48A
Auto-Pure
20A
Auto-Pure
20B
Auto-Pure
24D
Auto-Pure
24BT
Principle
Magnetic Particle Method
Throughput
1~32
1~48
1~20
1~20
1~24
1~24
Kits
96
Deep-well
plate
96
Deep-well
plate
2ml Kits
5ml Kits
10ml Kits+
2ml tubes
5ml Kits+
2ml tubes
Sample
Volume/μL
50~1000
50~1000
50~3000
50~5000
50~10000
50~5000
Stability
CV≤5%
Lysis temp.
Ambient temperature ~120℃
Elution temp.
Ambient temperature ~120℃
Heating time
Heating time（Ambient temperature ~120℃）≤4 minutes
Temperature
Accuracy
±1℃
Operation
interface
7-inch touch screen, 3 shortcut buttons and mouse is available
Built-in protocol
8 groups of preset protocols, 100 groups of protocols can be stored
Protocol
management
New, Edit, Delete, Save as
Expansion
interface
Standard USB, ethernet port and WIFI are available
Network
Extensible Ethernet remote control, WiFi, 4G network
Pollution control
UV light
Exhaust way
By Fan
Data storage
Available, with built-in SD card
Max.input
power
450W

Auto-Pure series Operation Manual Chapter 2 Specification
─3─
Dimension
(W×D×H)
400mm×470mm×450mm
400mm×520mm×450mm
Weight（kg）
28kg
30kg
3. Overall Dimension
Dimension（W×D×H）
Auto-Pure 32A：400mm×470mm×450mm
Auto-Pure 48A：400mm×470mm×450mm
Auto-Pure 20A：400mm×520mm×450mm
Auto-Pure 20B：400mm×520mm×450mm
Auto-Pure 24D：400mm×520mm×450mm
Auto-Pure 24BT：400mm×520mm×450mm

Auto-Pure series Operation Manual Chapter 3 Product Introduction
─4─
Chapter 3 Product Introduction
This chapter basically introduce the instrument structure, operation buttons,
display panel as well as the preparations before operate. For the first time
user, please make sure to read this chapter before start.
1. Structure
1.1. Front
1.2. Back
Display
screeen
Shortcut key
Operation Window
Socket
Fuse
Power Switch
USB port
Ethernet port
Debuggling port
Fan

Auto-Pure series Operation Manual Chapter 3 Product Introduction
─5─
1.3. Auto-Pure 20A/20B/24D/24BT Inside view
Note: The only difference between the experiment module of above
products is the shape of the heating strip.
1.4. Auto-Pure 32A/48A Inside view
Note: The only difference between the experiment module of above
products is the shape of the heating strip.
Magnetic rod
(Over the magnetic rod
cover)
Magnetic rod cover stand
Drawer
Heating strip
UV light
(top inside)
Magnetic rod
(Over the magnetic rod cover)
Fan
(bottom inside)
Magnetic rod cover stand
Drawer
Heating strip
UV light
(top insid
Fan
(bottom inside)

Auto-Pure series Operation Manual Chapter 3 Product Introduction
─6─
2. Operation panel
Display screen: Operate by touch screen or mouse which connect with USB
port
TAB: Select for the shortcut program
RUN: Run for the shortcut program
STOP: Stop operating
Display screen
TAB
RUN
STOP

Auto-Pure series Operation Manual Chapter 4 Operation
─7─
Chapter 4 Operation
1. Preparation
Take out the instrument from packing carton and tear off the tape on the
edge of operation window, then open it and take out the foam. First please
screw out the fixed screw of position ① as below .Second please screw out
the screw of position ② then you can take out the baffle and release the
moving component.
Note: Be careful in operating or the magnetic rod will be broken.

Auto-Pure series Operation Manual Chapter 4 Operation
─8─
2. Connect the power
Connect one end of power cord to the instrument socket and the other to power (AC100~240V), then
turn on the power switch.
3. Install the reagent kit
3.1Install the customized reagent kit for Auto-Pure 20A & Auto-Pure 20B.
Open the drawer and mount the customized reagent kit on the heating strip, make sure the sample
well marked with 1 should be on the left side, then push back the drawer slowly.
Note: The shape of customized reagent kit of above products is different, while the installation is the
same.
3.2Install the customized reagent kit for Auto-Pure 24D/24BT
Open the drawer and place the kits with sample on the kits tray, then put the kits tray to the drawer
and press the kits with hand to make sure the kits fits the heating strip well , then push back the
drawer slowly and close the door.
Customized reagent kit

Auto-Pure series Operation Manual Chapter 4 Operation
─9─
3.3Install the 96 well reaction plate on Auto-Pure 32A.
Take out the drawer and put the 96 well reaction plate which already filled with samples mounted on
the locating slot, make sure the plate with chamfering should be on the left side, then push back the
drawer slowly.
Customized
reagents kits
Kits tray
drawer
door

Auto-Pure series Operation Manual Chapter 4 Operation
─10─
4. Install the magnetic rod cover
4.1Install the magnetic rod cover for 20A/20B/24D/24BT
Insert the magnetic rod cover completely on the mounting groove, and the installation quantity
depends on the reagent kit number.
96 well reaction plate
Magnetic rod cover

Auto-Pure series Operation Manual Chapter 4 Operation
─11─
4.2Install the magnetic rod cover for Auto-Pure 32A/48A
Insert the magnetic rod cover completely on the mounting groove, and the installation quantity
depends on the reagent kit number.
Note: Magnetic rod cover for Auto-Pure32A at most is 4pcs, for Auto-Pure at most is 6pcs.The
installation method is same.
5. Remove magnetic rod cover
Press the button and take out the magnetic rod cover as per below photo.
Auto-Pure 32A
Magnetic rod cover

Auto-Pure series Operation Manual Chapter 4 Operation
─12─
6. Operation
6.1Start-up Interface
Turn on the instrument and make sure the drawer is closed before start, the screen will display the
start-up interface.
After start, it will enter into the shortcut mode as below:

Auto-Pure series Operation Manual Chapter 4 Operation
─13─
6.2Program Run
6.3Shortcut mode
Under the shortcut mode, select the program needed and click “Run”, it will enter into the program run
interface.
The program can also be selected by press “Tab” button on the panel and then press “Run” for start or
“Stop” for terminate.
Auto-Pure 20A Running interface

Auto-Pure series Operation Manual Chapter 4 Operation
─14─
Auto-Pure 20B Running interface
Auto-Pure 24BT Running interface