Amersham Frac-950 User manual

Fraction Collector
Frac-950
18-1139-56
User Manual
18113956_Front.fm Page 1 Wednesday, May 22, 2002 2:12 PM

18113956ae.book Page 2 Wednesday, May 22, 2002 1:55 PM

Important user information
All users must read this entire manual to fully
understand the safe use of Frac-950.
Amersham Biosciences AB, which was previously
known as Amersham Pharmacia Biotech AB, changed
its name to the current name on 17th October 2001.
WARNING!
The WARNING! sign highlights instructions
that must be followed to avoid personal
injury. It is important not to proceed until all
stated conditions are met and clearly
understood.
Caution!
The Caution! sign highlights instructions that
must be followed to avoid damage to the
product or other equipment. It is important
not to proceed until all stated conditions are
met and clearly understood.
Note
The Note sign is used to indicate information
important for trouble-free and optimal use of
the product.
CE Certifying
This product meets all requirements of applicable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of
conformity, is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked Amersham
Biosciences instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from
Amersham Biosciences except for
alterations described in this manual.
WARNING!
This is a Class A product. In a domestic environment
this product may cause radio interference in which case
the user may be required to take adequate measures.
Terms and Conditions of Sale
All goods and services are sold subject to the terms and
conditions of sale of the company within the Amersham
Biosciences group which supplies them. A copy of these
terms and conditions is available on request.
Should you have any comments on this product, we will
be pleased to receive them at:
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Trademarks
Drop Design, FPLC, UNICORN and ÄKTA are
trademarks of Amersham Biosciences Limited.
Amersham and Amersham Biosciences are trademarks
of Amersham plc.
Office Addresses
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Amersham Biosciences UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Biosciences Corp.
800 Centennial Avenue
PO Box 1327
Piscataway NJ 08855
USA
Amersham Biosciences Europe GmbH
Munzinger Strasse 9
D-79111 Freiburg
Germany
Amersham Biosciences K.K.
Sanken Building
3-25-1 Hyakunincho, Shinjuku-ku
Tokyo 169–0073
Japan
Amersham Biosciences China Limited
13/F., Tower I
Ever Gain Plaza
88 Container Port Road
Kwai Chung, New Territories
Hong Kong
© Copyright Amersham Biosciences AB 2002
- All rights reserved
Fraction Collector Frac-950 User Manual 18-1139-56 AE
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____________________________________________________ Contents
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE v
Contents
1 Introduction
1.1 General ...............................................................................................................7
1.2 Safety .................................................................................................................9
2 Installation
2.1 Unpacking ........................................................................................................10
2.2 General precautions ..........................................................................................10
2.3 Assembling tube racks ......................................................................................11
Assembling standard mode racks ..................................................................16
Assembling prep mode racks ........................................................................17
2.4 Installing a tube rack ........................................................................................18
Installing a standard mode tube rack ............................................................18
Installing a prep mode tube rack ..................................................................18
2.5 Installing capillaries ..........................................................................................19
General ........................................................................................................19
Using Frac-950 with DropSync ....................................................................19
Using Frac-950 with the accumulator ...........................................................21
2.6 Waste handling .................................................................................................23
2.7 Connecting electrical signal cables ....................................................................24
DropSync connector .....................................................................................24
Accumulator connector ................................................................................24
2.8 Connecting to a UniNet 1 communication link ................................................25
2.9 Connecting to a supply voltage .........................................................................26
2.10 Inserting collection tubes ..................................................................................27
2.11 Changing from standard mode to prep mode ...................................................28
General ........................................................................................................28
Before changing the components ..................................................................28
Removing standard mode components .........................................................29
Installing the Prep Mode Conversion kit ......................................................30
2.12 Installing the Funnel-to-flask kit .......................................................................34
2.13 Changing from prep mode to standard mode ...................................................36
Before changing the components ..................................................................36
Removing prep mode components ................................................................37
Installing standard mode components ..........................................................39
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Contents ____________________________________________________
Fraction Collector Frac-950
vi User Manual 18-1139-56 Edition AE
3 Operation
3.1 On/off .............................................................................................................. 40
3.2 Operating Frac-950 ......................................................................................... 41
3.3 Collecting fixed fractions ................................................................................. 42
3.4 Collecting peak fractions ................................................................................. 42
3.5 Feed tube ......................................................................................................... 42
3.6 Ignore_LastTube .............................................................................................. 43
3.7 Reset_Frac_Position ......................................................................................... 43
3.8 AccumulatorWash ........................................................................................... 44
3.9 Setting delay volume ........................................................................................ 45
3.10 Flow control during tube change ..................................................................... 46
3.11 Define rack and tube parameters ..................................................................... 47
4 Maintenance
4.1 Periodic maintenance ....................................................................................... 48
4.2 Cleaning and checking ..................................................................................... 48
4.3 Changing capillaries ........................................................................................ 48
4.4 Changing waste tubing .................................................................................... 48
5 Trouble-shooting
5.1 Faults and actions ............................................................................................ 49
Reference information
A Description ...................................................................................................... 51
Instrument ................................................................................................... 51
B Technical specifications ................................................................................... 53
Operating data ............................................................................................ 53
Tube racks ................................................................................................... 54
Physical data ............................................................................................... 55
C Accessories and consumables ........................................................................... 56
Short instructions
About this manual
This manual comprises two parts; a practical part (sections 1–5) and a
reference part (sections A-C). Sections 1–5 contain the necessary
information for operating the instrument.
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_________________________________________________ Introduction
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 7
1 Introduction
1.1 General
Fraction Collector Frac-950 is an automated fraction collector for use
in ÄKTA™ design chromatography systems. It is intended to be
operated as an integrated part of an ÄKTAdesign chromatography
system running UNICORN™software. Version 3.21 or higher is needed
for standard mode, 4.0 or higher for prep mode.
Frac-950 is equipped with an accumulator to eliminate spillage at high
flows. A drop sensor that can be used to control tube change at low
flows is also included.
Frac-950 is delivered with one standard mode rack. Different types of
racks accommodating different sizes and types of tubes are available as
options (see Section 2.3 for details).
Accessories for collecting extra large fraction volumes in prep mode are
also available (see Section 2.3 for details).
Figure 1-1. Fraction collector Frac-950, standard mode
FRAC 950
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Introduction _________________________________________________
Fraction Collector Frac-950
8User Manual 18-1139-56 Edition AE
Figure 1-2. Fraction collector Frac-950, prep mode
Frac-950 features:
• Collection of up to 392 fractions
- time or volume base
- fixed volume and/or peak fractionation
- fixed capillary tip - moving rack (standard mode)
- fixed rack - moving capillary tip (prep mode)
- fractionation order:
row-by-row
column-by-column
serpentine-row
serpentine-column
• Choice of six different collecting racks—four for standard mode
collection and two for preparative (prep) mode. One 18/30 mm
tube rack for standard mode collection is supplied with Frac-950.
The others are optional accessories.
• Two different methods for reducing spillage during tube change:
DropSync or Accumulator.
FRAC 950
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_________________________________________________ Introduction
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 9
1.2 Safety
• The unit is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Operate in accordance with local safety instructions.
WARNING! Pinch hazard. Do not have any part of your body
within the unit base area when Frac-950 is switched on. An
automatic calibration process starts when Frac-950 is connected to
UNICORN. During calibration, the dispenser arm moves rapidly.
Several beeps are heard before the calibration procedure starts.
WARNING! Be sure to fold down the safety bar whenever the rack
holder is operated by hand. This blocks the rack holder from
accidentally moving while the rack is moved by hand or replaced.
WARNING! The unit must be connected to a grounded mains
socket.
WARNING! When using hazardous chemicals, all suitable
protective measures, such as protective glasses, must be taken.
WARNING! When using hazardous chemicals, take care to
avoid spillage during fraction collection, when the rack holder is
moved by hand and when the rack is replaced.
WARNING! The unit must not be opened by the user. It contains
high voltage circuits that can deliver a lethal electric shock.
CAUTION! Always lift Frac-950 by the base unit, NEVER by the
safety bar, delivery arm or rack holder, as this may damage the unit.
CAUTION! Only spare parts approved or supplied by Amersham
Biosciences may be used for maintaining and servicing
the unit.
CAUTION! The DropSync unit will be damaged if it is positioned
below the tube rims.
Pinch
Hazard
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Installation __________________________________________________
Fraction Collector Frac-950
10 User Manual 18-1139-56 Edition AE
2 Installation
2.1 Unpacking
Unpack the unit and check the items against the supplied packing list.
Inspect the items for obvious damage which may have occurred during
transportation.
Retain all packing materials if onward transport of the unit is expected.
To make it easier to move Frac-950 on the laboratory bench, first lift
the front (approx. 30°) and tilt the unit until the rubber feet clear the
bench. Then move the unit to the desired location.
2.2 General precautions
The unit should not be installed in a corrosive atmosphere.
The unit should be located in a place of low temperature variations,
away from heat sources, draughts and direct sunlight.
The unit may be operated at temperatures in the range +4 to +40 °C.
The unit should be installed on a stable laboratory bench. The
recommended position for Frac-950 is immediately to the right of the
ÄKTAdesign chromatography system, as shown in the example below.
Figure 2-1. Frac-950 placed together with an ÄKTAdesign system
CAUTION! Always lift Frac-950 by the base unit, NEVER by the
safety bar, delivery arm or rack holder, as this may damage the unit.
FRAC 950
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__________________________________________________ Installation
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 11
2.3 Assembling tube racks
There are four standard mode racks and two prep mode racks available, as shown in the
following table. Each rack type has its own logical rack definition in UNICORN.
Frac-950 is delivered with rack type 18 and 30 mm tubes. The other
rack models are available as accessories.
Note: Installing prep mode racks requires a Prep mode conversion kit,
see 2.11 Changing from standard mode to prep mode.
Figures 2-2, 2-3, 2-4 and 2-5 show the tube patterns for the seven
different logical rack definitions available in UNICORN.
Rack
designation Rack type Color Tube combination Max. tubes Max.tube height
Standard mode
A18 and 30 mm
tubes Yellow 12x10 pos. 18 mm tubes 2x4 pos. 30 mm tubes 120 8115 mm 130 mm
B12 mm tubes Violet 16x15 pos. 12 mm tubes 240 100 mm
C
Microplates 96
1
and
30 mm tubes
1The following manufacturers microplates are tested and approved by Amersham
Biosciences for use with this rack type:
Greiner low: 655101, 651101, 650101
Greiner high: 780201
Nunc low: 143761, 168055, 156545, 163320
The difference between these microplates is the bottom shape and that a lid is included in some cases. All have 96
wells.
2 The funnel-to-flask rack is actually the prep mode 30 mm tube rack but only 30 of the 80 holes are filled with funnels.
Blue 4x96-well microplates
2x4 pos. 30 mm tubes 384 850 mm 136 mm
30 mm tubes Red 45 pos. 30 mm tubes 45 115 mm
Prep mode
E30 mm tubes Yellow 80 pos. 30 mm tubes 80 115 mm
F250 ml bottles Green 20 pos. 250 ml bottles 20 180 mm
G30 mm funnels2
funnel-to-flask Yellow 30 pos. 30 mm funnels 30 –
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Installation __________________________________________________
Fraction Collector Frac-950
12 User Manual 18-1139-56 Edition AE
Figure 2-2. Tube patterns as shown in UNICORN Method editor
Rack A: 18 and 30 mm tubes, yellow
Rack B: 12 mm tubes, violet
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__________________________________________________ Installation
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 13
Figure 2-3. Tube patterns as shown in UNICORN Method editor
Rack C: Microplates 96 and 30 mm tubes, blue
Rack D: 30 mm tubes, red
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Installation __________________________________________________
Fraction Collector Frac-950
14 User Manual 18-1139-56 Edition AE
Figure 2-4. Tube patterns as shown in UNICORN Method editor
Rack E: 30 mm tubes, yellow
Rack F: 250 ml bottles, green
The only
available
option
for this
rack type
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__________________________________________________ Installation
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 15
Figure 2-5. Tube patterns as shown in UNICORN Method editor
Rack G: 30 mm funnels, funnel-to-flask, yellow
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Installation __________________________________________________
Fraction Collector Frac-950
16 User Manual 18-1139-56 Edition AE
Assembling standard mode racks
A complete standard mode rack model assembly consists of a bowl, a
tube support and a tube holder as shown in Figure 2-6.
Figure 2-6. Rack model 18 and 30 mm tubes parts
1 Push the tube holder and tube support combination onto the bowl,
noting the keying guides on the bowl. The surface of the holder
should be level.
2 All other standard mode rack models are assembled in a similar
way.
Bowl
Tube
holder
with
Tube
support
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__________________________________________________ Installation
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 17
Assembling prep mode racks
A complete prep mode rack model assembly consists of a rack, 4 rack
legs and a bar.
Figure 2-7. Assembling Rack model 30 mm tubes
Assemble the rack as shown in Figure 2-7.
1 Fasten the two rack legs with guide pins to the bar using the
supplied screws (1).
2 Fasten all rack legs to the rack using the Phillips screws (2). Put the
plugs in place (3).
Plug
Phillips screw
Rack
Bar
Rack legs
(3)
(1)
(2)
Note: Place the legs with guide pins
towards the side with the bulge.
Guide pin
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Installation __________________________________________________
Fraction Collector Frac-950
18 User Manual 18-1139-56 Edition AE
2.4 Installing a tube rack
Installing a standard mode tube rack
1 Make sure the safety bar is folded down.
2 Fit the rack to the rack holder using the keying guide on the holder
to position it correctly, see Figure 2-8. Turn the rack on the holder
until it snaps firmly in position.
Note: The rack is kept in place on the holder with magnetic force.
Figure 2-8. Fitting the rack to the rack holder
3 Check that the tube numbering text on the tube holder is readable
from left-down.
4 Insert tubes gently in the tube holder as desired making sure the
tubes are inserted completely.
5 Fold up the safety bar.
Installing a prep mode tube rack
Note: Installing the prep mode racks requires a Prep mode conversion
kit. See 2.11 Changing from standard mode to prep mode.
Keying guide
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__________________________________________________ Installation
Fraction Collector Frac-950
User Manual 18-1139-56 Edition AE 19
2.5 Installing capillaries
General
Frac-950 is delivered with capillaries to suit all ÄKTAdesign systems.
Select the capillaries to suit your ÄKTAdesign system:
The capillaries are fitted with standard ÄKTA finger-tight connectors,
code no. 18-1112-55.
Using Frac-950 with DropSync
To install the capillary:
1 Connect a capillary from the chromatographic system outlet. Use a
standard ÄKTA connector. Tighten with your fingers only.
Note: For ÄKTAexplorer and ÄKTApurifier, this is port 2 on the
outlet valve. For ÄKTAbasic and ÄKTAFPLC™in standard config-
uration, this is the outlet from the Flow restrictor FR-904.
2 Use the standard ÄKTA connector on the DropSync unit to connect
the free end of the capillary to Frac-950 as shown in Figure 2-9.
3 Loosen the knurled screw to lower the DropSync unit to improve
access. Thread the capillary end through the DropSync unit and the
finger-tight connector.
4 Allow the capillary end to stick out approximately 2 mm (indicated
on the DropSync unit plastic housing) and tighten with your fingers
only. The capillary end should be straight and cleanly cut.
5 Adjust the height of the DropSync unit to suit the height of the
tubes to be used. Fasten the knurled screw.
Tubing i.d. mm Material/color ÄKTAdesign system
0.25 PEEK/Blue ÄKTAexplorer 10
ÄKTApurifier 10
ÄKTAbasic 10
0.50 PEEK/Orange ÄKTAexplorer 10/100
ÄKTApurifier 10/100
ÄKTAbasic 10/100
ÄKTAFPLC
0.75 PEEK/Green ÄKTAexplorer 100
ÄKTApurifier 100
ÄKTAbasic 100
ÄKTAFPLC
1.00 PEEK/Beige ÄKTAexplorer 100
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Installation __________________________________________________
Fraction Collector Frac-950
20 User Manual 18-1139-56 Edition AE
Figure 2-9. Installing a capillary with DropSync
6 Set operation parameters as follows:
•GotoSystem:Settings:Specials in UNICORN.
•In
FracParameters, change the DelayVol parameter.
The ÄKTAdesign Optional Configurations User Manual for
each ÄKTAdesign system describes how to calculate the
parameter values.
• Select Tube or DropSync as TubeChange parameter.
CAUTION! The DropSync unit will be damaged if it is positioned
below the tube rims.
Knurled screw
Finger-tight
connector
Capillary
Capillary end
projection
~ 2 mm
Capillary projection
indication on DropSync unit
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