AmScope 330 Series User manual

330
Series
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7
User’s Manual

330
Series
T
abl
e
o
f
Contents
2
Before Use............................................................................................................................... 3
Introduction..............................................................................................................3
Safety Precautions...................................................................................................4
Parts................................................................................................................................ 5
B330.................................................................................................................5
T330 .................................................................................................................6
Definition of Parts ............................................................................................... 7
Getting Started............................................................................................................8
Assembly .................................................................................................................8
Operation..................................................................................................................10
Setting Up ..................................................................................................................10
Adjusting the Diopter...............................................................................................10
Adjusting the Interpupillary Distance....................................................................10
Focusing.................................................................................................................11
Adjusting The Condenser & Diaphragm .......................................................... 12
Using the Trinocular Port .................................................................................. 13
Attaching a Camera ........................................................................................... 13
Setting the Stage’s Stop-Limit ..............................................................................14
Changing the Bulb............................................................................................. 14
Changing the Fuse ............................................................................................. 15
Maintenance/Precautions......................................................................................16
Specifications ............................................................................................................... 17
Specifications.........................................................................................................17
Optional Accessories.............................................................................................18
Objectives.......................................................................................................... 19
Technical Parameters ................................................................................................20
Electrical System............................................................................................... 20
Parameters..............................................................................................................20
Technical Terms & Concepts..........................................................................21
Troubleshooting........................................................................................................22

330
Series
Before
Use
3
Introduction
Congratulations on the purchase of your new AmScope microscope!
This manual is designed for the 330 series microscopes (B330 & T330).
Though the two models have identical bases, the B330 is equipped with a binocular head (two
eyepieces tubes), while the T330 is equipped with a trinocular head (two eyepiece tubes and a
third photo port on the top).
Please take a few minutes to familiarize yourself with the features and functions of your new mi-
croscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
www.iScopeCorp.com
We highly recommend you study this manual thoroughly before operating the microscope and
that you keep it on hand for future reference.
If you have additional questions or need assistance, please send us an email at:
[e-mail]

330
Series
Before
Use
4
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transportation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry
and clean place with temperatures between 32-100 degrees F (0-40 degrees C), and in
maximum relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consistent with
the voltage of your microscope.

330
Series
Parts
5
B330
Binocular Head
Eyepiece
Objectives
Mechanical Stage
ris Adjustment Slider
Coarse Focusing
Knob
Fine F
ocusing
Knob
Stage Control
Dimmer
Power Switch
Stage Clip
Lock Screw
Base Lens

330
Series
Parts
6
T330
Trinocular Head
Eyepiece
Objectives
Mechanical Stage
Condenser Up/Down
Knob
Coarse F
ocusing
Knob
Fine Focusing
Knob
Stage Control
Stage Clip
Trinocular Port
Base Lens

330
Series
Parts
7
Definition of Parts
AdjustableTrinocularPort
Threaded port with adjustable length of attachment of digital or video camera
Base Lens
Directs the light source towards the slide
Coarse FocusingKnob
Used to initially bring the slide into the sight and focus
Condenser &IrisDiaphragm
Controls the amount of light that hits the slide
Condenser Screw
Screws the condenser in place, securing it to the mechanical stage
Dimmer
Controls the amount of light that escapes from the base lens
Diopter
Allows the focus to be perfected for both eyes, independent of each other
Fine Focusing Knob
Used to refine focus to clear image
Head Lock Screw
Screws the microscope head into place
Limit Stop Knob
Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective
Mechanical Stage
echanically moves the slide along an X and Y axis for optimal positioning
Nosepiece
Houses the objective lenses
Rotating Head
Adjusts eyepieces to fit the distance between the user’s eyes for added comfort
Tension Knob
Adjusts the tension of the focusing knobs

330
Series
Getting Started
8
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Microscope Body
- One Binocular (B330) or One Trinocular (T330) Head
- One Trinocular Photo Port (for T330 only)
- Four Infinity Objectives (4x, 10x, 40x, 100x)
- 10x Widefield Eyepieces
- 16x Widefield Eyepieces (for -A models only)
- 20x Widefield Eyepieces (for -B models only)
- 25x Widefield Eyepieces (for -C models only)
- Blue, Green Color Filters
- One Mirror for Power Outage
- One Bottle of Immersion Oil
- One Spare Fuse
- One Spare 6V/20W Halogen Bulb
- One Dust Cover
3. Remove the microscope body from the box and remove the plastic protective covering. The body of the micro-
scope is composed of the base, the stage, the arm, and the nosepiece.
Fig. 1
4. Assembling the Head
1) Loosen the head lock-screw
①
fully.
2) From a little right position, insert the coattail interface on
the bottom of head into the hole of middle head with a
little left inclined. Keep the two eyepiece tubes forward,
and then tighten the lock screw.
For the T330 model, screw the photo port onto the top of
the microscope head (trinocular C-mount port

330
Series
Getting Started
9
Fig. 2
5. Assembling the Eyepiece
1) Remove the cover of eyepiece tube ① .
2) Insert the eyepiece ② into the eyepiece tube until
touching the bottom. (See Fig. 2)
Fig. 3
6. Assembling Objective
1) Rotate the coarse focusing knob to lower the stage.
2) Install the objective into the microscope nosepiece from
the lowest magnification to the highest in a clockwise
direction from the rear. (See fig.3)
Fig. 4
7. Plug in the microscope and turn it on. User dimmer to
adjust light.
Dimmer

330
Series
Operation
10
Setting Up
1. Loosen the head lock screw and adjust the
microscope head so that it is in a comfortable
position for observation. Lock the head-lock
screw.
Fig. 4
2. Adjusting the Diopter
1) After the image is clear in the right eyepiece, observe
the left eyepiece with the left eye. Rotate the diopter
adjustment ring ① until the image is clear (See Fig.
4).
2) There are ±5 diopters on the diopter adjustment ring,
and the value aligned with the scale is your eye’s
diopter.
★ Remember your eye’s diopter so that you can use it
next time.
Fig. 5
3. Adjust the Interpupillary Distance
1) When using both eyes to observe, hold the bases of the
prism and pull or push them to adjust the interpupillary
distance until there is only one field of view. (See Fig. 5)
2) Adjustable range: 52~75mm.
3) The number is the interpupillary distance.
★ Remember your eye’s interpupillary distance so that you
can use it next time.
4. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage,
holding it snugly in place with the metal slide holders (clips) of the mechanical stage.
5. Using the mechanical stage’s slide controls, center the specimen over the stage opening, lining it
up with the light and the objective lens.
Diopter Adjustment

330
Series
Operation
11
6. To adjust the illumination, slowly turn the dimmer knob on the right side of the body until the
desired intensity of light is achieved. You may also adjust the iris on the light source itself by
rotating the ring around the base lens or the iris on the condenser, so please make sure all are
adjusted to the correct positions.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x
objective) to locate and focus on the specimen. As you move up in magnification you may need
to refocus the image a little each time.
2. When using the 100x objective, a drop of immersion oil should be placed between the cover slip
and the objective to minimize distortion caused by air. Always be sure to wipe the oil off of the
objective with either lens cleaning paper or a nonabrasive, lint free cloth.
3. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your
other eye. Use the coarse focusing knob to adjust the height of the stage until the sample comes
into clear focus.
Note: You may loosen the limit-stop screw (located rear of the mechanical stage) in order to give
yourself a larger range of motion for fine tuning the focus.
4. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for
best results.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample,
or if moving the stage very close to the objectives. The limit stop is designed to prevent impact
between objective and slide, so when it is off you will be able to damage the microscope. For
safety, when using the 40x and 100x objectives, engage the limit stop once you have it close or in
contact with the objective (if using oil contact is required).

330
Series
Operation
12
Adjusting the Condenser & Diaphragm
Fig. 6
1. Using the condenser Up/Down
adjustment knob, you can change the
distance between the light condenser and
the stage. This allows you to control the
concentration of the light hitting your
slide.
2. By changing the aperture (hole size) of
the iris diaphragm, you can adjust the
background brightness. Adjust the
aperture of the iris diaphragm using the
iris adjustment slider located directly
under the stage.
Fig. 7
Fig. 8
3. You can take out condenser and replace
with the plan-concave reflecting mirror.
4. To use a color filter, swivel the filter
holder out, towards you. You can now
place the desired color filter into the
circular opening. Slide the filter holder
back to original position before
observation. (Fig. 8)
Note: The filter holder is placed in from the factory in a manner in which it swings out and hits the
arm of the microscope (backwards). If this happens, simply grip the condenser assembly and rotate
it. It may take a small amount of force to rotate it, but after doing so, you will be able to swing the
filter holder out towards the front of the unit for easier operation.
Condenser
Up/Down
ris Diaphragm
Adjustment Slider

330
Series
Operation
13
Using the Trinocular Port
The AmScope T690 model is uniquely designed so that you can view the image through the
eyepieces and the trinocular port simultaneously.
This feature allows the images through the microscope’s eyepieces, and those displayed on your
computer screen or television be viewed at the same time (although unless using 20x eyepieces to
match your camera, the image may differ in magnification). You do not need an adapter to attach
your AmScope camera to the trinocular port, however you may need one if you have a non-AmScope
camera. Our photo port is a 23mm size.
Attaching a Camera
1. If your camera has a C-mount, simply screw the camera onto the trinocular port.
2. If your camera has a 23mm mounting size, remove the C-mount from the top of the trinocular port by
loosening the screw. Then, drop the camera directly into the trinocular port. It should slide in without
issue.
3. To focus through the trinocular port, simply turn middle portion of the tube.
Fig. 9 Attaching camera on Binocular head Fig. 10 Attaching camera on Trinocular port

330
Series
Operation
14
Setting the Stage’s Stop-Limit
Fig. 11
1. Unlock the stop-limit, which is the switch inside the
left hand focusing knob. (Fig. 11 shows unlock
position)
2. Adjust the stage to the desired maximum height.
3. Lock the stop-limit. This will allow you to
limit the movement of the stage from the
bottom of the range up to the point that it is
set at.
4. To reset it, unlock the stage and reset the
stage to the new height that you would like
it to limit at. If no limit is desired, simply
unlock the stop-limit, but be careful when
using the oil immersion lens (100x), as with
no limit you can hit the slide, damaging the
lens or your sample.
Changing the Bulb
Fig. 12
1. Before changing the light bulb, first pull
the plug out of the electrical socket and wait
for the lamp to cool down. The light can get
hot when a halogen system is in use, so
please be careful to avoid being burned.
2. Please be sure to remove the eyepieces
from the unit before turning it upside down to
prevent them from falling and breaking.
3. Remove the door at the bottom of the microscope
by unscrewing it.
Stage Limit
Lock

330
Series
Operation
15
Fig. 13
4. You may now change the bulb by removing
and replacing it. Be sure that the bulb is not
hot before touching it to avoid burning.
Reinstall door and continue use of
microscope.
Replace Fuse
Fig. 14
1. Before replacing the fuse, make sure the power is
off and disconnect the power cord.
2. Unscrew the fuse housing at the bottom and
replace with a new one.
3. Place back the fuse housing.
Fuse

330
Series
Operation
16
Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a hand blower or
gently wiped off with a soft lens paper tissue/nonabrasive lint free cloth.
- Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).
- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.
- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.
- After use, cover the microscope with the provided dust cover.
- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damages.

330
Series
Specifications
17
330 Series Specifications
Parts Specifications B/T330 B/T330A B/T330B B/T330C
Extreme WF Eyepiece WF10X/23mm xxxx
WF10X/23mm w/ Pointer
WF10X/23mm w/ Reticle
WF16X/23mm x
WF20X/23mm
x
WF25X/23mm
x
DIN Achromatic Objectives 4X/0.10 xxxx
10X/0.25 xxxx
40X(spring)/0.65 xxxx
60X(spring)/0.85
100X(spring,oil)/1.25 xxxx
DIN Plan Objectives 4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head Binocular Sliding, 360 Degree Swiveling x (if B-) x (if B-) x (if B-) x (if B-)
Trinocular Sliding, 360 Degree Swiveling x (if T-) x (if T-) x (if T-) x (if T-)
TrinocularPort
C-Mount Photoport x (if T-) x (if T-) x (if T-) x (if T-)
23mm Photo Port x (if T-) x (if T-) x (if T-) x (if T-)
Focusing
Nosepiece
Coaxial Focusing System xxxx
Quadruple Nosepiece xxxx
Stage 120mm x 110mm Mechanical Stage xxxx
Movement Range: 70mm x 30mm xxxx
Condenser Abbe, NA= 1.25 xxxx
Diaphragm Iris Diaphragm xxxx
Illumination Halogen xxxx
LED
Lamp 6V/20W xxxx
LED
Filter Blue//Green xxxx
Blue

330
Series
Specifications
18
Optional Accessories
Parts Description Model # Purpose
Eyepieces 20x EP20X30 Obtaining 80x, 200x, 800x, and 2000x magnifica-
tion powers
25x EP25X30 For obtaining 250x and 2500x magnification pow-
ers
10x w/ Pointer EP10X30P For easier identifying of objects
10x w/ Reticle EP10X30R For measuring objects
Objective 4X PA4X-INF
10X PA10X-INF
40X PA40X-INF
100X PA100X-INF
Camera CMOSDigital MU035 (350k)
MU130 (1.3mp)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
To capture images, video, or view live display on a
computer (PC/Mac OS X)
Calibration Micrometer MR400 To calibrate the camera software for on screen
measurements
CCD Digital (VGA, Trinocular
Only)
CCD-MT To view live display on a computer monitor (VGA)
CCD TV/Video (Trinocular
Only)
CCD-NP To view live display on a television (RCA)
Stage Warmer
TCS-100 For controlling stage temperature

330
Series
Specifications
19
Objectives
Type Magnification Model Number/
Numerical Aperture
(N.A.)
Medium Parfocal Distance (mm) Magnification Marks
(Color Ring)
DIN Achromatic
Objective (195mm)
4X A4X / .10 Air 45 Red
10X A10X / 0.25 Air 45 Yellow
40X A40X / 0.65 Air 45 LightBlue
100X A100X / 1.25 Cedar Oil 45 White
DIN Plan Objective
(195mm)
Plan 4X PL4X / .10 Air 45 Red
Plan 10X PL10X / 0.25 Air 45 Yellow
Plan 40X PL40X / 0.65 Air 45 LightBlue
Plan 100X PL100X/ 1.25 Cedar Oil 45 White

330
Series
T
echnica
l
Parameters
20
Electrical System
The electrical system of this series of microscope is for universal voltage. The light bulb is 6V/20W halogen.
100V~224V power supply 50/ 60Hz
Parameters
Magnification -AModel:40x-1600x
-BModel:40x-2000x
-C Model:40x-2500x
Field of View Φ0.8mm~Φ4.5mm
Mechanical Tube Length 165mm
Object to Primary Image Distance 195mm
Fine Focusing Sensitivity 0.002mm