AmScope HHD2100HD-P User manual

HHD2100HD-P Handheld Digital Microscope
Introducon
This manual applies to the HHD2100HD-P digital microscope. The informaon in this manual is provided to help you
familiarize yourself with the assembly and use of the product. Please read thoroughly before using the products, and
keep this manual with the product for reference.
v1.0.202308

Safety
Before using your AmScope microscope, please read the following safety precauons carefully to avoid causing damage to
your AmScope product, or injury to yourself or others.
Turn o power if the instrument exhibits unusual or dangerous behavior such as eming smoke or unusual odors. These
can be indicaons of electrical problems, in which case the instrument should be disconnected from any power source if
safe to do so. Other indicators can be a loud buzzing sound or crackling. Contact AmScope to report such behavior.
Do not use around ammable liquids or gases. Electric instruments can ignite ammable substances which could result
in an explosion or re.
Do not use in a wet environment. Electrical components of the instrument can discharge when exposed to water, poten-
ally resulng in damage to the instrument, or injury to yourself or others.
Only use the provided power adapter or authorized replacement. Incompable power adapters can cause damage to the
instrument. If you should lose the included power adapter, please contact AmScope for informaon about a replacement.
Do not dismantle. Dismantling can result in damage to the instrument, and potenal exposure to dangerous materials or
electric current.
Noces
AmScope reserves the right to change specicaons of the hardware and soware at any me without noce. Connuous
eorts are made to improve performance and reliability, which can result in changes to design and compability. Please
contact AmScope for any concerns regarding such changes.
Proposion 65 Noce for California Residents
Cables included with the products described in this manual can expose you to chemicals including lead,
which is known by the state of California to cause cancer, birth defects or other reproducve harm. Visit
www.P65Warnings.ca.gov for more informaon.
Trademark Informaon
Windows is a trademark of Microso Corporaon. Mac OS (macOS) is a trademark of Apple Inc., and iOS is a trademark owned by
Cisco and leased by Apple Inc.. Linux is a trademark of Linus Torvalds. Android is a trademark of Google LLC. AmScope is a trademark
of United Scope LLC.

Contents
Specicaons.......................................................................................................................................................... 4
HHD2100HD-P ...................................................................................................................................................... 4
Model Specicaons ............................................................................................................................................. 5
Interface Specicaons ......................................................................................................................................... 6
Field of View and Working Distances ...................................................................................................................... 7
General Informaon............................................................................................................................................... 8
Zoom Wheel....................................................................................................................................................................... 8
LED Ring Light..................................................................................................................................................................... 9
Using Support Systems..................................................................................................................................................... 10
Quick Start Guide ................................................................................................................................................. 11
AmScope Imaging Soware ......................................................................................................................................... 11
Setup............................................................................................................................................................................ 11
HDMI .................................................................................................................................................................... 12
User Interface................................................................................................................................................................... 12
The Camera Control Panel ............................................................................................................................................... 13
Image Exposure................................................................................................................................................................ 14
Manual Exposure .................................................................................................................................................................................................. 14
Auto Exposure....................................................................................................................................................................................................... 14
Color................................................................................................................................................................................. 14
White Balance....................................................................................................................................................................................................... 14
The Measurement Toolbar............................................................................................................................................... 15
The Camera Control Toolbar ............................................................................................................................................ 17
Sengs............................................................................................................................................................................. 17
Measurement ....................................................................................................................................................................................................... 17
Magnicaon........................................................................................................................................................................................................ 18
Image Format........................................................................................................................................................................................................ 18
Video..................................................................................................................................................................................................................... 19
Storage.................................................................................................................................................................................................................. 19
Language............................................................................................................................................................................................................... 19
Miscellaneous ....................................................................................................................................................................................................... 20

HHD2100HD-P User Guide
Page 4
1. Image Snap Buon
2. Video Record Buon
3. Hand Grip
4. Zoom Wheel
5. Polarizaon Wheel
6. HDMI Port
7. USB Mouse Port
8. 12VDC Power Port
9. Micro Secure Digital Reader/Recorder
1 2
3
4
5
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6
HHD2100HD-P

HHD2100HD-P User Guide
Page 5
Model Specicaons
Model HHD2100P-HD
Zoom Magnicaon 0.1X-3.3X
Sensor IMX307 (color)
Sensor Type CMOS
Sensor Opcal Format 1/2.8”
Integrated Reducon 0.5X
Acve Pixels 2M (1920 x 1080)
Pixel Size 2.9µm x 2.9µm
Acve Sensor Area 5.57mm x 3.13mm
Shuer electronic rolling shuer
Sensivity 1300mV @ 1/30s, f/5.6
Exposure Time 0.01ms - 1000ms
Spectral Response 380-650nm with IR-cut lter
Capture Resoluon and Maximum Framerate 60fps @ 1920x1080 (HDMI)
50-60fps @ 1920x1080 (SD)
Connecvity HDMI video out (v1.4 standard)
USB input (mouse)
Media Reader/Writer Secure Digital (SDIO 3.0 standard)
Video Format H264, H265 encoded MP4
Power 12VDC, 1A
Operang Condions -10°C to 50°C, 30%-80% relave humidity
Storage Condions -20°C to 60°C, 10%-60% relave humidity

HHD2100HD-P User Guide
Interface Specicaons
Port Layout
HDMI Port Complies with HDMI1.4 standard, 1080P format video output for standard FHD
monitor
USB Mouse Connect the included USB Wireless mouse for easy operaon with embedded
XCamView soware
Power Output 12V/1A
LED LED status indicator
SD Complies with SDIO3.0 standard. SD card is used to save, store, and transfer
images and video
Camera Hardware
Sensor Sony IMX307(C), 1/2.8"(5.57x3.13), Pixel size 2.9x2.9um
G Sensivity /Dark Signal/Dynamic Range /SNR 1300mv with 1/30s/NA/NA/NA
FPS/Resoluon 60@1920*1080(HDMI)
Exposure Time 0.01~1000ms
Soware
Video Saving Video format: 2MP(1920*1080) H264 encoded MP4 le
Frame Rate: 50~60fps (related with SD card performance);
Image Capture 2MP (1920*1080) JPEG image in SD card
Measurement Saving Measurement informaon saved in dierent layer with image content;
Measurement informaon is saved together with image content in burn in
mode.
ISP Funcon Exposure(Automac / Manual Exposure) / Gain, White Balance(Manual / Au-
tomac / ROI Mode), Sharpening, 3D Denoise, Saturaon Adjustment, Con-
trast Adjustment, Brightness Adjustment, Gamma Adjustment, Color to Gray,
50HZ/60HZ An-icker Funcon
Image accessibility Zoom In/Zoom Out, Mirror/Flip, Freeze, Cross Line, Overlay, Embedded Files
Browser, Video Playback, Measurement Funcon
Mulple Language Support English / Simplied Chinese / Tradional Chinese / Korean / Thai / French
/ German / Japanese / Italian
Polarizaon and Lighng
Brightness Control Adjust the light intensity through soware GUI
Polarizaon adjustment Manually adjust the polarizaon direcon 0-90 degrees

HHD2100HD-P User Guide
Magnicaon Working Distance Field of View (X) mm Field of View (Y) mm
10 134.63 48.09 27.07
20 73.65 24.05 13.54
30 50.85 16.03 9.02
40 40.39 12.02 6.77
50 33.51 9.62 5.41
60 29.30 8.02 4.51
70 26.02 6.87 3.87
80 23.76 6.01 3.38
90 21.84 5.34 3.01
100 20.32 4.81 2.71
110 19.18 4.37 2.46
120 18.14 4.01 2.26
130 17.33 3.70 2.08
140 16.58 3.44 1.93
150 15.97 3.21 1.80
160 15.40 3.01 1.69
170 14.90 2.83 1.59
180 14.49 2.67 1.50
190 14.09 2.53 1.42
200 13.75 2.40 1.35
210 13.43 2.29 1.29
220 13.15 2.19 1.23
230 12.88 2.09 1.18
240 12.65 2.00 1.13
250 12.42 1.92 1.08
260 12.21 1.85 1.04
270 12.03 1.78 1.00
280 11.85 1.72 0.97
HHD2100HD-P Magnicaons, Working Distances, and Fields of View
System Magnicaon
Digital microscopes oen present smaller elds-of-view when compared to opcal microscopes with equivalent
magnicaons. This is due to the digital microscope’s image sensor. The dimensions of the sensor determine the eld-of-
view much like the aperture of an eyepiece (usually printed on the eyepiece as its eld number). Each model will list its
sensor’s dimensions which can be used to calculate the eld-of-view.

HHD2100HD-P User Guide
Zoom Wheel
The zoom wheel controls the magnicaon of the microscope by adjusng the internal zoom mechanism. The wheel
can be rotated in a connuous movement unl either terminus has been reached. By rotang the wheel to the right,
the magnicaon will decrease unl it reaches 0.1X. Rotang the wheel to the le will increase the magnicaon unl it
reaches 3.3X.
Zoom Wheel

HHD2100HD-P User Guide
8 LED Illuminator
The ring-light’s intensity can only be controlled via the built-in soware.

HHD2100HD-P User Guide
Using Support Systems
The HHD2100HD-P is designed for use with a variety of support systems, including those typically used with stereo
microscopes such as table stands, boom-arm stands, and arculang stands. Due to the HHD2100HD-P’s high magnica-
ons, it is recommended to always use a support to avoid shaking which will result in poor-quality images.

HHD2100HD-P User Guide
Quick Start Guide
The HHD2100HD-P portable HDMI interface digital microscopic all-in-one machine consists of 5G high-denion opcal
lenses, a 1080P HDMI camera, and an 8 LED polarizaon ring lightsource module. This model is designed for stand-alone
use – so no need for a computer. Connect it directly to an HDMI monitor or television for live viewing and recording.
You’ll be impressed by the responsiveness of the real-me video, and the smooth 60fps output. As a stand-alone system,
the microscope uses internal soware to provide on-screen controls for capturing photos and videos, with a built-in
Secure Digital reader/writer to store the les. Use the included mouse to navigate the microscope’s sengs and its large
assortment of eding and measuring tools.
AmScope Imaging Soware
AmScope imaging soware for Windows, Mac OS, and Linux is used to capture and edit images generated by the
microscope. While the microscope may work with 3rd-party UVC-compable soware, it is recommended to use the
provided soware to ensure full funconality. For the most up-to-date version, please visit the AmScope website at
www.amscope.com, and navigate to the soware download page.
Setup
1. Connect the camera to a HDMI monitor using the HDMI cable;
2. Insert the supplied Wireless USB mouse Receiver to the camera’s USB port;
3. Insert the supplied MicroSD card into the HDMI camera MicroSD card slot;
4. Connect the camera to the power adapter(USB5V/2A) and switch it on;

HHD2100HD-P User Guide
HDMI
User Interface
The HDMI model uses an on-screen UI to operate. The camera controls are located in the panel on the le side of the
window, the measurement toolbar on the top, and addional display controls and sengs are on the boom.
By default, the panels will only display when the mouse cursor is hovering over the appropriate area. Le-clicking
on the “pin” icon will maintain the panel’s visibility, but will prevent other panels from opening.
The HDMI model’s user interface

HHD2100HD-P User Guide
The Camera Control Panel
The Camera Control Panel controls the camera to achieve the best video or image quality according to the specic appli-
caons. To open the panel, move the mouse cursor toward the le edge of the video window.
Control Panel Funcon Descripon
Snap Capture image and save it to the microSD card.
Record Record video and save it to the microSD card.
Auto Exposure When Auto Exposure is checked, the system will automacally
adjust exposure me and gain according to the value of expo-
sure compensaon.
Exposure Compen-
saon
Available when Auto Exposure is checked. Slide to increase or
decrease the overall brightness to compensate for varying sub-
jects.
Exposure Time Available when Auto Exposure is not checked. Slide to increase
or decrease exposure me for achieving appropriate brightness.
Gain Available when Auto Exposure is not checked. Slide to increase
or decrease signal amplicaon. Increasing the amplicaon will
increase brightness without adjusng exposure me, but will
result in increased noise levels.
Red Manually adjust the proporonal Red level of the RGB video.
Green Manually adjust the proporonal Green level of the RGB video.
Blue Manually adjust the proporonal Blue level of the RGB video.
Auto (White Bal-
ance)
Connuously adjusts RGB levels automacally based on the
overall video content.
Manual (White
Balance)
Enables the manual RGB controls to adjust the color balance.
ROI (White Bal-
ance)
Use the on-screen Region-of-Interest selector (rectangle will
appear) to dene a region which should be used as reference
when performing white balance.
Sharpness Slide to adjust the sharpness level of the video.
Denoise Slide to adjust the amount of noise-reducon to apply to the
video.
Saturaon Slide to adjust the color saturaon of the video.
Gamma Slide to increase or decrease the Gamma level of the video.
Contrast Slide to adjust the contrast of the video.
DC Select when using DC powered illuminaon. No icker compen-
saon.
AC (50Hz) Select when using AC powered illuminaon with 50Hz to re-
move ickering.
AC (60Hz) Select when using AC powered illuminaon with 60Hz to re-
move ickering.
Default Restore all values in the Camera Control Panel to their default
sengs.

HHD2100HD-P User Guide
Image Exposure
Manual Exposure
Image exposure controls the amount of light captured by the imaging device. Exposure me is used to set the amount
of me over which light is collected. More me will result in a brighter image. For most stac subjects, increasing expo-
sure me to achieve the appropriate brightness is the preferred method. For moving subjects, too much me will result
in blurring as the subject’s locaon changes over me. Exposure me must be limited in these cases to prevent excess
movement. Use of the gain seng would therefore be the preferred method to achieve opmal brightness. Gain controls
the amount of amplicaon applied to the signal generated by the imaging sensor. While the signal strength would be
increased, noise generated during the imaging process would also be increased.
Auto Exposure
Auto exposure relies on soware to set the exposure me and gain. This simplies the workow, especially when captur-
ing images in changing lighng condions. As a microscope’s magnicaon changes, or the distance from the microscope
to the subject changes, the amount of light collected will also change. Auto exposure can connuously compensate for
these changes. This method assumes that the opmal amount of light would create an image with levels which would
average to a middle tone. Because of this, images of light subjects may appear too dark, and images of dark subjects may
appear too bright. For this reason, adjustment can be done using the exposure compensaon control, somemes called
exposure target. This allows you to increase or decrease the target level of brightness.
Color
White Balance
The color of an object is inuenced by the color of light reecng o of it or passing through it. Dierent light sources
produce light with dierent color-biases. Common examples are incandescent lamps which typically produce warm
colors compared to uorescent or LED lamps which produce cooler colors. This sort of bias is referred to as color tem-
perature. Due to this phenomenon, a white object may appear slightly yellow in morning sunlight, and slightly blue in
aernoon shade. To compensate for these variaons, digital imaging devices can process images using what is called
“white balance.” The white balance process shis colors based on the color of the light source, with the goal of produc-
ing a more neutral white or grey.

HHD2100HD-P User Guide
Icon Funcon
Pin the Measurement Toolbar
Show / Hide Measurement Objects
Select Measurement Unit
Select Magnicaon (requires calibraon process)
Object Selector
Angle
4-Point Angle
Point
Arbitrary-Angle Line
3-Point Lines
Horizontal Line
Vercal Line
Parallel
3 Points Vercal Line
Rectangle
Ellipse
5-Point Ellipse
Circle
3-Point Circle
Annulus (Concentric Circles)
The Measurement Toolbar
The Measurement Toolbar will pop up when moving the mouse cursor toward the upper edge of the video window. This
panel contains tools and sengs for performing measurements.

HHD2100HD-P User Guide
Icon Funcon
Two Circles with Distance from Centers
Two 3-Point Circles with Distance from Centers
Arc
Text
Polygon
Curve
Scale Bar
Arrow
Perform magnicaon calibraon. Denes relaonship between the measurement
unit and pixel size. Requires the use of a micrometer slide.
Export measurement informaon to CSV le
Delete measurement object
Exit measurement mode
Le-clicking on a measurement object will open the Object Locaon & Properes Con-
trol Bar. The icons on the control bar:
• Move Up
• Move Down
• Move Le
• Move Right
• Adjust Color
• Delete
When the Pin icon is clicked to lock the Measurement Toolbar, the Camera Control Panel will not be displayed unl
the Measurement Toolbar is unpinned by clicking the Exit icon .

HHD2100HD-P User Guide
Icon Funcon Icon Funcon
Zoom In Zoom Out
Horizontal Flip Vercal Flip
Color/Grayscale Freeze Video
Display Crosshair/Grid Browse Images and Videos on SD Media
Sengs Soware Version Informaon
The Camera Control Toolbar
The Camera Control Toolbar provides a subset of tools for controlling the camera’s video, as well as general sengs and
the le viewer.
Global Precision Sets the decimal scale: number of digits aer a decimal point
Calibraon Line Width Sets the width of lines used for calibraon
Color Sets the color of lines used for calibraon
Endpoint Sets the shape used at each endpoint of a line. Includes null or rectangle
Point, Angle, Line, Horizontal Line, Vercal Line, Rectangle, Circle, Ellipse, Annulus, Two Circles, Polygon, Curve
Each shape can be customized using aributes such as Line Width and Color
Sengs
Measurement
Measurement sengs allow you to customize shapes, and dene the precision of measurements.

HHD2100HD-P User Guide
Magnicaon
Magnicaon sengs allow you to edit stored magnicaon calibraons. Each me the calibraon process is completed,
you can save the results as a magnicaon preset. These presets can be selected from the Measurement Toolbar to use
the appropriate, calibrated sengs.
Name This is the user-dened name of magnicaon seng. Names typically incorporate the magnicaon of
the objecve lens used, and addional informaon pertaining to the microscope or camera used.
Resoluon The number of pixels per meter.
Clear All Clears all saved calibraon data
Delete Clears the selected calibraon data
Image Format
Image Format sengs allow you to determine whether measurement objects will be merged into saved images, or em-
bedded as a separate layer.
Measurement Object
Saving Method
Burn In Mode Measurement objects are merged with the image before saving the le.
Objects can neither be edited nor removed.
Layered Mode Measurement objects are saved in a separate layer, and can be edited.

HHD2100HD-P User Guide
Video
Video sengs allow you to change the fast-forward and reverse intervals, and to choose the video codec.
Playback Fast Forward/Reverse
Interval
The number of seconds to be skipped when fast-forwarding or reversing
playback of videos
Video Encode H264 Older AVC video codec with beer compability across mulmedia de-
vices, but less ecient compression compared to H265.
H265 Newer HEVC video codec with improved compression while retaining
high quality
Storage
Storage displays the format of the current SD card’s le system, such as FAT32, exFAT, and NTFS. Unformaed cards must
be formaed on a computer or other device.
Language
Language seng selects which language will be displayed.

HHD2100HD-P User Guide
Miscellaneous
Miscellaneous sengs include addional helpful sengs.
Auto Exposure Set the maximum exposure me for the Auto Exposure mode. Seng a lower number can
improve the frame rate.
ROI Color Select the color of the Region of Interest (ROI) frame
Camera Parameters
Import
Import previously-saved parameters from the SD card
Camera Parameters
Export
Export current parameters to the SD card
Reset to Factory defaults Restore all parameters to the factory-default values