AmScope 120 Series User manual

120
Series
1
User’s Manual

2
120
Series
T
abl
e
o
f
Contents
Before Use..........................................................................................3
Introduction ............................................................................................................. 3
Safety Precautions ................................................................................................... 4
Parts .............................................................................................. 5
B120 ................................................................................................................. 5
T120 ................................................................................................................. 6
Base & Condenser ............................................................................................ 7
Definition of Parts ...............................................................................................
Getting Started ............................................................................ 9
Assembly .................................................................................................................. 9
Operation .................................................................................. 10
Setting Up ................................................................................................................. 10
Focusing ................................................................................................................. 10
Adjusting The Condenser & Diaphragm ......................................................... 11
Attaching a Camera ........................................................................................... 11
Setting the Stage’s Limit-Stop .............................................................................. 11
Adjusting Focusing Tension ........................................................................... 12
Maintenance/Precautions ..................................................................................... 12
Specifications.............................................................................. 13
B120 Series Specifications .................................................................................... 13
T120 Series Specifications .................................................................................... 14
Optional Accessories ............................................................................................. 15
Objectives .......................................................................................................... 16
Eyepieces ................................................................................................................ 16
Technical Parameters ................................................................ 17
Electrical System ............................................................................................... 17
Parameters ............................................................................................................. 17
Technical Terms & Concepts .......................................................................... 1
Troubleshooting........................................................................ 19
Common Issues ..................................................................................................... 19
Common Issues (Continued) ............................................................................. 20

3
120
Series
Before
Use
Introduction
Congratulations on the purchase of your new AmScope microscope!
This manual is designed for the B120 series microscope.
Please take a few minutes to familiarize yourself with the features and functions of your new mi-
croscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
www.iScopeCorp.com
We highly recommend you study this manual thoroughly before operating the microscope and
that you keep it on hand for future reference.
If you have additional questions or need assistance, please send us an email at:
[e-mail]

4
120
Series
Before
Use
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transportation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry
and clean place with temperatures between 32-100 degrees F (0-40 degrees C), and in
maximum relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consistent with
the voltage of your microscope.

5
120
Series
Parts
B120
Eyepieces
Binocular Siedentopf Head
Nosepiece Turret
Objectives
Limit Stop
MechanicalStage
Coarse Focusing
Condenser
Fin e F o c u sin g
Iris Control
Base Lens
Mechanical Stage Controls
Dimmer

6
120
Series
Parts
T120
Trinocular C-Mount Port
Eyepieces
Nosepiece Turret
Objectives
MechanicalStage
Limit Stop
Coarse Focusing
Condenser
Fin e F o c u sin g
Iris Control
Base Lens
Mechanical Stage Controls
Dimmer

7
120
Series
Parts
Base & Condenser

8
120
Series
Parts
Definition of Parts
Base Lens
Directs the light source towards the slide
Coarse FocusingKnob
Used to initially bring the slide into the sight and focus
Condenser &IrisDiaphragm
Controls the amount of light that hits the slide
Condenser Screw
Screws the condenser in place, securing it to the mechanical stage
Dimmer
Controls the amount of light that escapes from the base lens
Diopter
Allows the focus to be perfected for both eyes, independent of each other
Fine Focusing Knob
Used to refine focus to clear image
LimitStopScrew
Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective
Mechanical Stage
Mechanically moves the slide along an X and Y axis for optimal positioning
Nosepiece
Houses the objective lenses
Rotating Siedentopf Head
Adjusts eyepieces to fit the distance between the user’s eyes for added comfort
Tension Knob
Adjusts the tension of the focusing knobs

9
120
Series
Getting
Started
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Siedentopf Microscope Head (Binocular or Trinocular)
- One Microscope Base
- Four DIN Standard Objectives (4x, 10x, 40x, 100x)
- 10x Widefield Eyepieces
- 16x Widefield Eyepieces (for -A models only)
- 20x Widefield Eyepieces (for -B models only)
- Blue Color Filters
- One Bottle of Immersion Oil
- One Spare Fuse
- One Dust Cover
- One Blue Color Filter
Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing.
3. Remove the microscope body from the box and remove the plastic protective covering. The body of the micro-
scope is composed of the base, the stage, the arm, the nosepiece, and the head.
4. Remove the eyetube caps and drop the desired eyepieces into the eyepiece ocular tubes. Be sure to avoid
touching the lens to ensure no artifacts appear in your image.
5. Screw the objectives into the microscope nosepiece from the lowest magnification to the highest, again avoid-
ing touching the lenses.
6. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on
the side of the base.

120
Series
Operation
10
Setting Up
1. Adjust the microscope head so that it is in a comfortable position for observation.
2. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyeubes and ro-
tating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both eyes.
(mounted on the Siedentopf head).
3. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.
4. Using the mechanical stage controls (vertical pillar with dual knobs under the right side of the stage), center the
specimen over the stage opening, lining it up with the light and the objective lens.
5. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of
light is achieved.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo-
cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each
time.
2. When using the 100x objective, a drop of immersion oil should be placed between the cover slip and the objec-
tive to minimize distortion caused by air. Always be sure to wipe the oil off of the objective with either lens cleaning
paper or a nonabrasive, lint free cloth.
3. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.
Note: You may loosen the limit-stop (located rear of the stage as a vertical thumb screw) in order to give yourself
the full range of motion for fine tuning the focus.
4. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if moving the
stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when
it is off you will be able to damage the microscope. For safety, when using the 40x and 100x objectives, engage the
limit stop once you have it close or in contact with the objective (if using oil contact is required) to prevent addi-
tional movement and damage.

120
Series
Operation
11
Adjusting the Condenser & Diaphragm
1. Using the condenser-adjustment knob (left of unit, under the stage), you can change the distance between the
light condenser and the stage. This allows you to control the concentration of the light hitting your slide.
2. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the
aperture of the iris diaphragm using the iris adjustment slider located directly under the stage.
3. If you want to use a color filter, swivel the filter holder out, towards you. You can now place the desired color
filter into the circular opening. Slide the filter holder back to original position before observation.
Note: The filter holder is placed in from the factory in a manner in which it swings out and hits the arm of the
microscope (backwards). If this happens, simply grip the condenser assembly and rotate it. It may take a small
amount of force to rotate it, but after doing so, you will be able to swing the filter holder out towards the front of
the unit for easier operation.
Attaching a Camera
If you have also purchased a camera for this unit, all you need to do is remove the eyepiece from the ocular tube
and slide the camera (with reduction lens installed) into the vacant tube.
Setting the Stage’s Limit-Stop
1. Unlock the limit-stop on the rear stage (unscrew the lower bolt in the assembly).
2. Adjust the limit-stop to the desired maximum height.
3. Lock the stop-limit (screw in the bottom bolt in the assembly). This will allow you to limit the movement of the
stage from the bottom of the range up to the point that it is set at.

120
Series
Operation
12
Adjusting Focusing Tension
1. To adjust the tension of the focusing knobs, first locate the black ridged tension knob on the inside of the coarse
focusing knob.
2. To decrease tension, rotate the adjustment forward, towards the stage (counterclockwise). To increase, rotate
away from the stage (clockwise).
Note: If your stage is slipping down after setting the focus, you need to increase the tension.
Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a hand blower or
gently wiped off with a soft lens paper tissue/nonabrasive lint free cloth.
- Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).
- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.
- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.
- After use, cover the microscope with the provided dust cover.
- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damages.

13
120
Series
Specifications
B120 Series Specifications
Parts Specifications B120A B120B B120C
WF Eyepiece
WF10X/18mm xxx
WF10X/18mm w/ Pointer
WF10X/18mm w/ Reticle
WF16X/13mm x
WF20X/10mm x
WF25X x
Plan Eyepiece
P5X
P10X
P16X
DIN Achromatic Objectives
4X/0.10 xxx
10X/0.25 xxx
40X(spring)/0.65 xxx
60X(spring)/0.85
100X(spring,oil)/1.25 xxx
Plan Objectives
4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head
Binocular Sliding,360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
30 Degree Viewing Head
Binocular Sliding,360 Degree Swiveling xxx
Trinocular Sliding, 360 Degree Swiveling
Focusing
Nosepiece
Coaxial Focusing System xxx
Quadruple Nosepiece xxx
Stage
128mm x 127mm Mechanical Stage xxx
Movement Range: 70mm x 30mm xxx
Condenser
Abbe, NA= 1.25 xxx
Diaphragm
Iris Diaphragm xxx
Illumination
Halogen Light w/ Dimmer
LED Light w/ Dimmer xxx
Lamp
6V/20W
6V/30W
LED xxx
Filter
Blue xxx

14
120
Series
Specifications
T120 Series Specifications
Parts Specifications T120A T120B T120C
WF Eyepiece
WF10X/18mm xxx
WF10X/18mm w/ Pointer
WF10X/18mm w/ Reticle
WF16X/13mm x
WF20X/10mm x
WF25X x
Plan Eyepiece
P5X
P10X
P16X
DIN Achromatic Objectives
4X/0.10 xxx
10X/0.25 xxx
40X(spring)/0.65 xxx
60X(spring)/0.85
100X(spring,oil)/1.25 xxx
Plan Objectives
4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head
Binocular Sliding,360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
30 Degree Viewing Head
Binocular Sliding,360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling xxx
Focusing
Nosepiece
Coaxial Focusing System xxx
Quadruple Nosepiece xxx
Stage
128mm x 127mm Mechanical Stage xxx
Movement Range: 70mm x 30mm xxx
Condenser
Abbe, NA= 1.25 xxx
Diaphragm
Iris Diaphragm xxx
Illumination
Halogen Light w/ Dimmer
LED Light w/ Dimmer xxx
Lamp
6V/20W
6V/30W
LED xxx
Filter
Blue xxx

15
120
Series
Specifications
Optional Accessories
Parts Description Model # Purpose
Eyepiece 5X EP5X23 For obtaining 20x, 50x, 200x, and 500x magnifica-
tion powers
20x EP20X23 For obtaining 80x, 200x, 800x, and 2000x magnifi-
cation powers
25x EP25X23 For obtaining 250x and 2500x magnification pow-
ers
10x w/ Pointer EP10X23P For easier identifying of objects
10x w/ Reticle EP10X23R For measuring objects
Objective 2X A2X For obtaining 20x and 32x magnification powers
5X A5X For obtaining 50X and 80X magnification powers
20X A20x For obtaining 200x and 320x magnification powers
60X A60X For obtaining 600x and 960x magnification powers
Plan 4X PA4X For obtaining higher clarify in images
Plan 10X PA10X For obtaining higher clarify in images
Plan 40X PA40X For obtaining higher clarify in images
Plan 100X PA100X For obtaining higher clarify in images
Darkfield Condenser Dry Darkfield Condenser DK-DRY100 For obtaining low power darkfield images
Oil Darkfield Condenser DK-OIL100 For obtaining high power darkfield images
Camera CMOS Digital MU035 (350k)
MU130 (1.3mp)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
To capture images, video, or view live display on a
computer (PC/Mac OS X)
Calibration Micrometer MR400 To calibrate the camera software for on screen
measurements
Phase Contrast Kit Simple PCS Phase contrast images
Turret PCT High quality phase contrast images with ease of use
Stage Warmer
TCS-100 For controlling stage temperature

16
120
Series
Specifications
Objectives
Type Magnification Numerical Aperture
(N.A.)
Medium Parfocal Distance (mm) Magnification Marks
(Color Ring)
DIN Achromatic
Objective (195mm)
4X A2X Air 45 Red
10X A5X Air 45 Yellow
40X A20x Air 45 LightBlue
60X A60X Air 45 Deep Blue
100X A100X Cedar Oil 45 White
Plan Objective
(195mm)
Plan 4X PA4X Air 45 Red
Plan 10X PA10X Air 45 Yellow
Plan 40X PA40X Air 45 LightBlue
Plan 100X PA100X Cedar Oil 45 White
Eyepieces
Type Widefield Eyepiece
Medium
Plan Eyepiece
Magnification 10X 15X 20X 5X 10X 16X
Field of View
Φ 18 Φ 13 Φ 11 Φ 18 Φ 18 Φ 15

17
120
Series
T
echnica
l
Parameters
Electrical System
There are two options for electrical systems for this series of microscope. The light source is dependent on
which model you have, but can either be a 6V/20W halogen, a 6V/30W halogen, or an LED system.
1. 220V~240V power supply: 220V~240V ±10%, 50Hz
This electrical system is CE and GS certified
2. 100V~120V power supply: 100V~120V ±10%, 60Hz
This electrical system is UL certified.
All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent
on which unit is purchased.
Parameters
Magnification -AModel:40x-1600x
-BModel:40x-2000x
Field of View Φ0.8mm~Φ4.5mm
Mechanical Tube Length 165mm
Object to Primary Image Distance 195mm
Fine Focusing Sensitivity 0.002mm

18
120
Series
T
echnica
l
Parameters
Technical Terms & Concepts
TotalMagnification
Total magnification of a microscope is calculated by the magnification of the objective multiplied by the
magnification of the eyepieces.
-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification
Field of View
Linear field of view of the eyepiece divided by the magnification of the objective
Numerical Aperture (N.A)
Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the
features of the objective and condenser’s image quality and resolution. The “n” is a refractive index of the
medium (air or immersion cedar oil) between the objective lens and the specimen. The “ α ” is 1/2 of the angle
between the aperture on the objective and path of light. The larger the N.A, the higher the resolution of the
objective (and better quality of the image) is.
Object to Primary Image Distance
The distance between the object plane and the primary image plane. The conjugate distance is fixed.
Mechanical Tube Length
The distance between the objective shoulder and the ocular shoulder

19
120
Series
T
roubleshooting
Common Issues
Symptom Cause Remedy
OPTICAL ISSUES
One side of the field
of view is darker
The nosepiece if misaligned Turn the nosepiece until it clicks into place
Stains or dust has accumulated on the condenser,
objective, eyepieces, or base lens
Clean all lenses with lens cleaner or a lint free non-
abrasive cloth
Obstructions are
observed in the field
of view
Stains, dust, or dirt has accumulated on the speci-
men
Clean the slide or use a new specimen if sample is
destroyed
Stains, dust, or dirt have accumulated on the lens Clean the lens
UnclearImage There is no cover slip on the slide Add a cover slip. The objectives are designed for use
with a 0.17mm cover slip, so it is a requirement to
use one for proper images.
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm
thickness slip
The immersion oil has accumulated on the dry
objective
Thoroughly clean the objective lens with lens clean-
er or a lint free nonabrasive cloth
No immersion oil is used with the 100x objective Use immersion oil for better clarity and resolution
Air bubble in the immersion oil Pop the air bubble
Used wrong oil Use standard cedarwood oil
The aperture is no open to an appropriate diam-
eter
Adjust the aperture to have the light just larger than
the size of the condenser
Stain or dust has accumulated on the lens in the
inlet of the head
Clean the lens with lens cleaner or a nonabrasive
lint free cloth, as well as spray with compressed air
The condenser is not in the right position Adjust condenser height to the top of the travel
range, then adjust down to focus image
One side of the field
of view is dark or the
image moves while
focusing
The specimen slide is not fixed Secure the slide to the stage with clips
The nosepiece is not in the right position Turn the nosepiece until it clicks into place
The field of view is not
bright enough
The iris diaphragm is not big enough Adjust the iris diaphragm to allow the light to be
just larger than the condenser
The condenser is not in the right position Adjust condenser height to the top of the travel
range, then adjust down to focus image
Stains, dust, or dirt has accumulated on the con-
denser, objective, eyepieces, or base lens
Thoroughly clean tall lenses with lens cleaner or a
lint free nonabrasive cloth

120
Series
T
roubleshooting
20
Common Issues (Continued)
Symptom Cause Remedy
OPTICAL ISSUES
The color of the image is
notaccurate
The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color
clarity
No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired
filter
(TrinocularOnly)
I cannot see anything in the
camera / Image is black
The trinocular port shutter of the microscope is not open Slide the silver metal pin out on the head of the microscope
to allow light to reach the trinocular port
MECHANICALISSUES
The objective touches the
cover slip
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip
The limit-stop is set too high or not engaged Be careful to avoid contact between objective and the slide when
the limit stop is up (unless using the 100x objective with oil). To
reengage, focus the sample, then lock the limit stop into place at
the stage height to set max height at a safe but usable distance.
Unable to move the slide
smoothly
The slide is not secured correctly Adjust the slide to use the stage clips and secure the sample
The mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage
Focus knob does not turn The tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus
knob counterclockwise (close to the arm of the microscope on
the left of the microscope)
Stage declines by itself The tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus
knob clockwise (close to the arm of the microscope on the left
of the microscope)
The coarse focusing knob
won’t raise the stage
Limit-stop is engaged Disengage the limit stop (move up) on the read of the stage of the
microscope inside the coarse focusing knob
The fine focusing knob
won’t raise the stage
Limit-stop is engaged Disengage the limit stop (move up) on the read of the stage of the
microscope inside the coarse focusing knob
ELECTRICAL ISSUES
The bulb/lightsource
flickers
The bulb is close to burning out The unit’s LED bulb should not burn out. The bulb is not the
issue. Please contact customer service for warranty repair infor-
mation.
The microscope does not
light up
The microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina-
tion
The bulb burned out The unit’s LED bulb should not burn out. The bulb is not the
issue. Please contact customer service for warranty repair infor-
mation.
The fuse burned out Replace with fuse on the bottom of the microscope
The fuse burns out fre-
quently
The voltage is too high Use the correct power supply (110v if 110v unit, 220v if 220v
unit), or get a voltage adapter to convert to the proper electrical
system
The bulb burns out fre-
quently
The voltage is too high Use the correct power supply (110v if 110v unit, 220v if 220v
unit), or get a voltage adapter to convert to the proper electrical
system