AmScope 660 Series User manual

660
Series
1
User’s Manual

2
660
Series
T
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Contents
Before Use..........................................................................................3
Introduction ............................................................................................................. 3
Safety Precautions ................................................................................................... 4
Parts .............................................................................................. 5
B660 ................................................................................................................. 5
T660 ................................................................................................................. 6
Definition of Parts ............................................................................................... 7
Getting Started ............................................................................ 8
Assembly ..................................................................................................................
Operation .................................................................................... 9
Setting Up ................................................................................................................... 9
Focusing ................................................................................................................... 9
Adjusting The Condenser & Diaphragm ......................................................... 10
Using the Trinocular Port ................................................................................. 10
Attaching a Camera ........................................................................................... 10
Setting the Stage’s Stop-Limit .............................................................................. 11
Adjusting Focusing Tension ........................................................................... 11
Changing the Bulb ............................................................................................. 11
Maintenance/Precautions ..................................................................................... 12
Specifications.............................................................................. 13
Specifications ......................................................................................................... 13
Optional Accessories ............................................................................................. 14
Objectives .......................................................................................................... 15
Technical Parameters ................................................................ 16
Electrical System ............................................................................................... 16
Parameters ............................................................................................................. 16
Technical Terms & Concepts .......................................................................... 17
Troubleshooting........................................................................ 18
Common Issues ..................................................................................................... 1
Common Issues (Continued) ............................................................................. 19

3
660
Series
Before
Use
Introduction
Congratulations on the purchase of your new AmScope microscope!
This manual is designed for the 660 series microscopes (B660 & T660).
Though the two models have identical bases, the B660 is equipped with a binocular head (two
eyepieces tubes), while the T660 is equipped with a trinocular head (two eyepiece tubes and a
third photo port on the top).
Please take a few minutes to familiarize yourself with the features and functions of your new mi-
croscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
www.iScopeCorp.com
We highly recommend you study this manual thoroughly before operating the microscope and
that you keep it on hand for future reference.
If you have additional questions or need assistance, please send us an email at:
[e-mail]

4
660
Series
Before
Use
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transportation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry
and clean place with temperatures between 32-100 degrees F (0-40 degrees C), and in
maximum relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consistent with
the voltage of your microscope.

5
660
Series
Parts
B660
Binocular Head
Eyepieces Head Lock Screw
Objectives
Power Switch
MechanicalStage
Light Dimmer
Iris Adjustment Slider
Diaphragm Centering Controls Coarse Focusing Knob
Base Lens Iris
Fine Focusing Knob
Mechanical Stage Controls

6
660
Series
Parts
T660
Trinocular Port
Trinocular Head
Head Lock Screw
Eyepieces
Objectives
Power Switch
MechanicalStage
Light Dimmer
Iris Adjustment Slider
Diaphragm Centering Controls
Coarse Focusing Knob
Base Lens Iris
Fine Focusing Knob
Mechanical Stage Controls

660
Series
Parts
7
Definition of Parts
AdjustableTrinocularPort
Threaded port with adjustable length of attachment of digital or video camera
Base Lens
Directs the light source towards the slide
Coarse FocusingKnob
Used to initially bring the slide into the sight and focus
Condenser & IrisDiaphragm
Controls the amount of light that hits the slide
Condenser Screw
Screws the condenser in place, securing it to the mechanical stage
Dimmer
Controls the amount of light that escapes from the base lens
Diopter
Allows the focus to be perfected for both eyes, independent of each other
Fine Focusing Knob
Used to refine focus to clear image
Head Lock Screw
Screws the microscope head into place
Limit Stop Knob
Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective
Mechanical Stage
Mechanically moves the slide along an X and Y axis for optimal positioning
Nosepiece
Houses the objective lenses
Rotating Head
Adjusts eyepieces to fit the distance between the user’s eyes for added comfort
Tension Knob
Adjusts the tension of the focusing knobs

8
660
Series
Getting
Started
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Microscope Body
- One Binocular (B660) or One Trinocular (T660) Head
- One Trinocular Photo Port (for T660 only)
- Four Infinity Objectives (4x, 10x, 40x, 100x)
- 10x ExtremeWidefieldEyepieces
- 15x Extreme Widefield Eyepieces (for -A models only)
- 20x Extreme Widefield Eyepieces (for -B models only)
- 25x Extreme Widefield Eyepieces (for -C models only)
- Three Color Filters (Blue, Green, & Yellow)
- One Bottle of Immersion Oil
- One 6V/30W Spare Halogen Bulb (for 30W models only)
- One Spare Fuse
- One Dust Cover
3. Remove the microscope body from the box and remove the plastic protective covering. The body of the micro-
scope is composed of the base, the stage, the arm, and the nosepiece.
4. Loosen the metal knob located directly above the nosepiece (the head-lock screw) completely. Place the head
(flat, circular side down) into the circular opening on top of the arm. Tighten the head-lock screw to secure the
head in place.
5. For the T660 model, screw the photo port onto the top of the microscope head (trinocular C-mount port).
6. Remove the eyetube caps and drop the desired eyepieces into the eyepiece ocular tubes. Be sure to avoid
touching the lens to ensure no artifacts appear in your image.
7. Screw the objectives into the microscope nosepiece from the lowest magnification to the highest, again avoid-
ing touching the lenses.
8. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob near
the power switch.

660
Series
Operation
9
Setting Up
1. Loosen the head lock screw and adjust the microscope head so that it is in a comfortable position for observa-
tion. Lock the head-lock screw.
2. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyepiece tubes
and rotating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both
eyes.
3. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.
4. Using the mechanical stage’s slide controls, center the specimen over the stage opening, lining it up with the light
and the objective lens.
5. To adjust the illumination, slowly turn the dimmer knob on the right side of the body until the desired intensity
of light is achieved. You may also adjust the iris on the light source itself by rotating the ring around the base lens
or the iris on the condenser, so please make sure all are adjusted to the correct positions.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo-
cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each
time.
2. When using the 100x objective, a drop of immersion oil should be placed between the cover slip and the objec-
tive to minimize distortion caused by air. Always be sure to wipe the oil off of the objective with either lens cleaning
paper or a nonabrasive, lint free cloth.
3. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.
Note: You may loosen the limit-stop knob (located on the inside of the coarse focusing knob on the left side of the
microscope) in order to give yourself the full range of motion for fine tuning the focus.
4. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if moving the
stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when
it is off you will be able to damage the microscope. For safety, when using the 40x and 100x objectives, engage the
limit stop once you have it close or in contact with the objective (if using oil contact is required).

660
Series
Operation
10
Adjusting the Condenser & Diaphragm
1. Using the condenser-adjustment knob, you can change the distance between the light condenser and the stage.
This allows you to control the concentration of the light hitting your slide.
2. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the
aperture of the iris diaphragm using the iris adjustment slider located directly under the stage.
3. If you want to use a color filter, swivel the filter holder out, towards you. You can now place the desired color
filter into the circular opening. Slide the filter holder back to original position before observation.
Note: The filter holder is placed in from the factory in a manner in which it swings out and hits the arm of the
microscope (backwards). If this happens, simply grip the condenser assembly and rotate it. It may take a small
amount of force to rotate it, but after doing so, you will be able to swing the filter holder out towards the front of
the unit for easier operation.
Using the Trinocular Port
The AmScope T660 model is uniquely designed so that you can view the image through the eyepieces and the
trinocular port simultaneously.
This feature allows the images through the microscope’s eyepieces, and those displayed on your computer screen
or television be viewed at the same time (although unless using 20x eyepieces to match your camera, the image
may differ in magnification). You do not need an adapter to attach your AmScope camera to the trinocular port,
however you may need one if you have a non-AmScope camera. Our photo port is a 23mm size.
Attaching a Camera
1. If your camera has a C-mount, simply screw the camera onto the trinocular port.
2. If your camera has a 23mm mounting size, remove the C-mount from the top of the trinocular port by loosening
the screw. Then, drop the camera directly into the trinocular port. It should slide in without issue.
3. To focus through the trinocular port, simply turn middle portion of the tube.