AmScope IN480 Series User manual

IN480 Series
Inverted Biological
Microscope
INSTRUCTIONS
This manual is for the using method, troubleshooting and maintenance about
IN480 series biological microscope. Please study this manual thoroughly before
operating, and keep it with the instrument. The manufacturer reserves the rights to
the modifications. Technical specifications may be subject to changes without
notice.

Before Use
1.Components..............................................................................................................1
2.Assembling ...............................................................................................................3
2-1 Assembling Scheme........................................................................................3
2-2 Assembling Steps ................................................................................................4
3.Adjustment Device....................................................................................................8
4.Using Adjustment Device ....................................................................................... 10
4-1 Stand..................................................................................................................10
4-2 Stage..................................................................................................................11
4-3 Observing Tube.................................................................................................12
4-4 Illumination Device......................................................................................14
5.Phase-contrast observation.................................................................................... 17
5-1 Nomenclature....................................................................................................17
5-2 Assembling and operating............................................................................ 18
6.TV device & Photography device........................................................................... 20
6-1 TV device......................................................................................................20
6-2 Photography Device..................................................................................... 21
7.Technical Specifications.........................................................................................23
7-1 Technical Parameters...................................................................................23
7-2 Parameters of Objective............................................................................... 23
8.Troubleshooting...........................................................................................................24
Contents IN480 Series

1. Operation Notice
Fig. 1
Fig. 2
1. As the microscope is a high precision
instrument, always operate it with care, and
avoid physical shake during the operation.
2. Do not expose the microscope in the sun
light directly, neither in the high temperature,
damp, dust or acute shake. Make sure the
worktable is flat and horizontal.
Operating environment: temperature
5℃~40℃, maximum relative humidity 80%.
3. When moving the microscope, use both hands
to hold its stand arm ②and the front base
①, and lay it down carefully (see Fig. 1).
4. The microscope power should be grounded to
avoid lightning strike.
5. For safety, make sure the main switch③is at
“O” (off) and power it off before replacing
the bulb or fuse. Wait until the lamp cools
down.
★Bulb selected only: 6V/30W Halogen light
(Philips 5761).
6. Universal voltage is supported as 90~240V.
Additional transformer is not necessary. Make
sure the voltage is in this range.
7. Use the special cord supplied by our company.
2. Maintenance
1. Wipe the lens gently with a soft tissue. Carefully wipe off the oil marks and fingerprints
on the lens surfaces with a tissue moistened with a small amount of 3:7 mixture of
alcohol and ether or dimethylbenzene.
★As alcohol and ether is flammable, don’t place these chemical near to fire or fire
source. When turning on or off the electrical device, place these chemicals in a
ventilated place.
2. Don’t use organic solution to wipe the surfaces of the other components. Please use the
neutral detergent if necessary.
3. If the microscope is damped by liquid when using, please power it off immediately and
wipe it dry.
4. Never disassemble the microscope by yourself.
5. After using, cover the microscope with a dust cover.
Before Use IN480 Series

IN480 Series
3. Safety Sign
Sign
Signification
Hot at surface, do not touch.
Study the instructions before use. Unsuitable operation would lead to
person hurt or instrument faulty.
|
Main switch ON
O
Main switch OFF

● Stage Extension Board
● Attachable Mechanical Stage
Koehler Illuminator
Light SourceObjective
Illumination
Holder
Phase-contrast Slider
LongWorking
DistanceCondenser
Binocular
ObservingTube
Stand Nosepiece
Stage
Eyepiece
Circular Central Stage
●
Metal Stage
● Glass Stage
IN480B
1.Components IN480 Series
1

IN480 Series
Eyepiece
Trinocular
Observing Tube
● Metal Stage
● Glass Stage
● Stage Extension Board
● Attachable Mechanical Stage
Nosepiece
Stage
Stand
Circular Central Stage
Phase-contrast Slider
Illumination
Holder
Objective
Koehler Illuminator
Light Source
IN480T
LongWorking
DistanceCondenser
2

紧固螺杆
物镜
2-1 Assembling Scheme
Following is the Assembling Scheme, and the numbers denote the assembling order.
★Before assembling, make sure there is no dust or dirt. Assemble carefully and do not scrap
any part or touch the glass surface.
★Save the hex spanner. It will be used when changing the parts.
⑨
Filter
N.A.0.5 Condenser
Lamp House Cover
②
N.A.0.3 Condenser
①
Eyepiece
●
PL10×
/22
●
PL15
×
/16
⑧
Halogen Bulb
6V 30W HAL
⑦
③
⑥
Lock-screw
Glass Stage
Metal Stage
④
○
10
Power Cord
⑤
Stand
Stage Extension Board
AttachableMechanical Stage
2.Assembling IN480 Series
3

2-2 Assembling Steps
Fig. 1
2-2-1 Assemble and change the bulb (see Fig.1)
◎Lamp selected only: 6V/30W HAL bulb
(Philips 5761)
★Do not touch the bulb with finger. If there
is a fingerprint, wipe it with clean and soft
cloth.
1. Hold the bulb ①with clean glove or gauze
and insert the pins ②to the jack ②. Make
sure the bulb is vertical.
★Insert the bulb gently to avoid damage.
2. Change the bulb. The bulb will become very
hot when using or after using. Before replacing
the bulb, turn off the main power (The main
switch is at “O” and disconnect the power
cord) and wait until the lamp holder and bulb
cool down.
2-2-2 Assemble Lamp House Cover (see Fig. 2)
1. Slide down lamp house cover①by groove
until the hole②on the cover①is aligned to
the screw hole②.
2. Tighten lock screw③into screw hole②.
Fig. 3
2-2-3 Assemble the Objective (see Fig.3&Fig.4)
1. Rotate the coarse focusing knob①to lower the
nosepiece to the lowest position (see Fig.3).
★★★★ To make the instrument safe in
transit, please set he nosepieces to the lowest
position after use. Please turn the adjusting
ring②of the coarse adjustment knob to the
proper tension. (see Fig.3)
2. Screw the objectives into the microscope
nosepiece from the lowest magnification to
the highest in a clockwise direction from the
rear.
IN480 Series
Fig. 2
4

IN480 Series
Fig. 5
Fig. 6
◎For ease of use, please following this
assemble direction.
◎It can also be assembled by aperture on the
stage.
★★★★ Clean the objectives periodically
because the inverted microscope objectives are
sensitive.
★★★★ Cover the hole on the nosepiece with
a dust cap③to avoid the dust and the dirt. (see
Fig.4)
★★★★ Search and focus the sample starting
from low magnification objective (4X or 10X).
Then change to the high power magnification
ones if needed.
★★★★ After the objective replaced, rotate the
objective nosepiece until it sounds “ka-da” to
make sure the objective is in the center of light
path.
2-2-4 Assemble the Stage extension board/
Attachable mechanical stage (see Fig.5&Fig.6)
◎Stage extension board could be assembled on
both left side and right side to extend the work
stage. But the stage extension board and the
attachable mechanical stage cannot be used at
the same side.
◎Attachable mechanical stage generally is
assembled on the right.
1. Assemble stage extension board. Tighten the
lock screw①on stage extension board. Then
screw it from bottom right or bottom left.
Use the small tools to tighten till the board
is installed firmly.(see Fig.5)
2. Assemble attachable mechanical stage
Assemble it as the stage extension board (see
Fig.6).
Fig. 4
5

IN480 Series
2-2-5 Circular central stage (see Fig.7)
1. Assemble the metal stage①on the stage
aperture. Turn the metal stage V②facing to
the user. It is easier to check the objective.
2. Lay glass stage flat when using it. There is
special direction.
Fig. 7
2-2-6 Assemble the Eyepiece (see Fig.8)
1. Remove the cover of eyepiece tube①.
2. Insert the eyepiece②into the eyepiece tube
fully.
Fig. 8
2-2-7 Assemble the Condenser (see Fig.9)
1. Loosen the condenser fixing rod②until
aligned with the condenser holder③inner
wall.
2. Push condenser①into condenser holder③
as arrow direction. Make it staying
vertically.
3. Tighten condenser fixing rod②.
Fig. 9
★★ N.A.0.3condenseris used with 4X~20X
low
magnificationobjective.
★★★★ N.A.0.5 condenser is used with above
40Xhighmagnificationobjective.
★★★★ Refer to 4-4-6 to replace condenser.
6

IN480 Series
2-2-8 Assemble the filter (see Fig.10)
★★★★ Make sure the filter has completely
cool down before changing it.
1. Place the filter①into the filter holder②.
2. Filter can overlap on another filter when
needed, but the thickness must not exceed
8mm.
Fig. 10
Fig. 11
2-2-9 Connect power cord (Fig.11, Fig.12 &
Fig.13)
★★★★ Do not exert force to power cord. It is
easy to be damaged bytwisting it.
1. Turn the main switch ①to “O” (OFF) before
connecting the power cord. (see Fig.11)
2. Insert the plug②into the socket②, and make
sure the connection is good. (see Fig.12)
3. Insert the other end④of the power cord to the
supply outlet⑤, and make sure the connection
is good. (see Fig.13)
★★★★ Use the special cord supplied by
our company. Use similar cord if lost or
damaged.
★★★★ Universal voltage is supported as
90～240V
★★★★ Connect the cord correctly. Make
sure it’s grounded.
2-2-10 Replace the fuse (see Fig.11&Fig.12)
Turn the main switch①to “O”(off) before
replacing the fuse. Pull out the power cord.
Screw out the fuse group⑥from the fuse base⑦
with a “-” screwdriver. Install a new fuse and
screw to the fuse base.
Fig. 13
★Specification: 250V, 3.15A。
Fig. 12
7

★Main Switch，Coarse TensionAdjusting Ring，Tumbler Lock Knob，Field DiaphragmAdjusting
Rod，Light Source Centering Knob is on the right of the equipment. See XD30Afor its location.
★Phase-contrast slide adopt phase center adjustable slide configured with phase center
presetting rod.
Filter Holder Phase-contractSlider
Phase Centering Rod
Condenser
Up-down Knob Diopter
Adjusting Ring
Condenser
Adjusting Rod InterpupillaryDistance
Pointer
BrightnessAdjustingKnob Coarse Focusing Knob
ApertureDiaphragm
Adjusting Rod
Frosted Glass
Flapper
Fine Focusing Knob
IN480B
3.Adjustment Device IN480Series
8

IN480 Series
★The Brightness Adjusting Knob and Frosted Glass Flapper is on the left of the equipment.
Light Source
Centering Knob
Field Diaphragm
Adjusting Rod
Filter Holder
Phase-contractSlider
Aperture Diaphragm
Adjusting Rod
Condenser
Up-down Knob
Interpupillary DistancePointer
Tumbler Lock Knob
Phase Center
Adjusting Rod
Condenser
Adjusting Rod
Main Switch
CoarseTension
Adjusting Ring Fine Focusing Knob
Coarse Focusing Knob
Condenser Lock Rob
DiopterAdjusting Ring
Light Path Shifting Block
IN480T
9

4-1 Stand
Fig. 14
4-1-1 Turn on the light (see Fig.14)
Connect the power cord. Turn on the main
switch①to “—” (on).
Fig. 15
Fig. 16
4-1-2 Adjusting brightness (see Fig.15)
Turn the dial①clockwise to brighten the light.
Turn the dial counter-clockwise to dim it.
◎Use dim light will increase the life of the
bulb.
4-1-3 Adjusting coarse tension adjusting ring
(see Fig.16)
★The coarse tension adjusting ring①is used
to adjust the tension of coarse adjustment
knob②.
Turn the coarse tension adjusting ring①
clockwise will tighten the Coarse adjusting
knob②; Turn the ring counter-clockwise
will loosen the knob. If the nosepiece goes
down by itself or the sample moves from the
focus after focusing by the fine focusing
knob③, the coarse focusing knob②is too loose
and should be tightened by turning the ring as
the figure shows.
4. UsingAdjustment Device IN480 Series
10

IN480 Series
4-2 Stage
Fig. 17
Fig. 18
4-2-1 Placing sample (see Fig.17&Fig.18)
Place the sample in the center of the stage.
★Please select the container like Petri dish,
culture flask etc, with the thickness matching
objective mark to get the best observe effects.
Select specimen slide with the thickness
matching objective mark when placing the
sample on slide.
◎Use φ35mm Petri dish
1. Place the φ35mm Petri dish holder ①
(attached) on the center of the circular
central stage.
2. Move the whole Petri dish holder to move
Petri dish.
◎UseAttachable mechanical stage
1. Extend the sample clip ②to clip the
microtitration plate when using ＃96 or ＃24
microtitration plate.
2. Use holder and attachable mechanical stage
as following:
●Use Terasaki holder ③to place Terasaki
board.
●Useφ35mm Petri dish holder④to place
φ35mm Petri dish.
●Use specimen slide holder⑤to place
φ54 Petri dish and specimen slide.
3. Turn horizontally shift ring⑥and vertically
shift ring⑦to move the specimen to proper
place.(Working distance range: 120mm×80mm)
4-2-2 Move the specimen
Turn the shift ring on attachable mechanical
stage or move specimen by hand to proper place.
★Be Careful when changing the objective to
avoid collision with the circular central stage or
the Petri dish holder. The working distance is
pretty short.
11

IN480 Series
4-3 Observing Tube
Fig. 19
4-3-1 Adjusting diopter (see Fig.19&Fig.20)
◎For IN480B
1. Turn coarse focusing knob and fine focusing
knob to focus the specimen when observing
the left eyepiece with left eye.
2. Then observe the right eyepiece with right
eye. Rotate the diopter adjustment ring①if
the image is unclear(see Fig.19)
★There is ±5 on the ring. The “.” marks the
number of your eye’s diopter.
★Remember your eye’s diopter to use it next
time.
Fig. 20
◎For IN480T
1. Turn coarse focusing knob and fine focusing
knob to focus the specimen when observing
the right eyepiece with right eye.
2. Then observe the left eyepiece with left eye
and rotate the diopter adjustment ring④
until image is clear. (See fig.20)
4-3-2 Adjusting Interpupillary distance (see
Fig.20& Fig.21)
Fig. 21
◎When using both eyes to observe, hold
the base of the prism and until there is only one
field of view. (see Fig.21)
◎The “·” ① on the side marks the number②
of the interpupillary distance. (see Fig.21)
Rang of interpupillary distance: 50~76mm
◎For IN480B, the marker is below the right
eyepiece base, see ①in Fig.21.
For IN480T, the Marker is above the left
eyepiece base, see ⑤ in Fig.20.
★
Remember your interpupillary distance so
that you can use next time.
12

IN480 Series
4-3-3 Use the eye-cap (see Fig.22)
1. If the user wears glasses, turn over the eye-
cap①to prevent the glasses from touching the
eyepiece and avoid damaging the glasses and the
eyepiece.
2. If the user doesn’t wear glasses, open the
eye-cap②. It can prevent stray light from
disturbing the observation.
Fig. 22
Fig. 23
4-3-4 Shift light path (see Fig.23)
◎For IN480T trinocular observation.
◎ Shift the light path by moving the light way
shifting block①.
◎Move the block to the right until hearing
a click when doing normal observation. Move
the block to the left until hearing a click when
taking photo or recording video.
Light path Shifting Block Light distribution rate
Apply
Shift to the right 100% use in binocular
observation Dark specimen observation
Shift to the left 50% use in binocular
observation, 50% use in
photo or video
Light specimen observation
and TV, photo or digital
video
13

4-4 Illumination Device
Color filter Purpose
IF550 Monochromaticcontrast filter (Green)
(Use in phase-contrast observation)
LBD
Colortemperature transition filter
(Use
in bright-field observation and
photomicrograph)
SIF800 De-infrared filter (Use in photomicrograph to compensate
the exposure time)
4-4-1 Using filters
◎Use filters to increase the accuracy of
the observation and photomicrography.
Suggest using LBD filter to get more
neutral hue when observing bright-field
and taking photo.
◎Filters can overlay on the filter holder.
Make
sure that the maximum thickness is less
than 8mm.
★Place the filter according to Fig.10 and
follow the instruction.
Fig. 24
Fig. 25
4-4-2 Centering the Condenser Group
1. Rotate the condenser up-down knob ①
to lower it to the lowest position (see
Fig. 24).
2. Rotate the objective 10X to the light
path and focus the specimen.
3. Rotate the field diaphragm adjustment
rod②to put the field diaphragm to
the smallest position.
4. Rotate the condenser up-down
knob①to adjust the image to the
clearest.
5. Adjust the condenser adjustment
rod②and put the image to the center
of the field of view.
6. Open the field diaphragm gradually. If
the image is in the center all the time
and inscribed to the field of view, the
condenser has been centered correctly
(see Fig.25).
7. In using, you can open the field
diaphragm a little bit to make the
image just a little larger than the field
of view.
IN480 Series
14

IN480 Series
Fig. 26
4-4-3 Adjusting the Field Diaphragm
By limiting the diameter of the beam entering the
condenser, the field diaphragm can prevent other
light and make the image contrast sharper. When
the image is just on the edge of the field of view,
the objective can show the best performance and
obtain the clearest image.
4-4-4 Centering the Light source (see Fig.26)
◎Pull out frosted glass flapper①. A djust
diaphragm to minimum by tuning condenser
aperture diaphragm adjusting rod②.
◎Observe the filament image on the aperture
diaphragm. Adjust light source centering
knob③to make filament image clearest and
centering in the diaphragm. Plug in frosted
glass flapper (see Fig.26).
Fig. 27
4-4-5 Using Aperture Diaphragm Adjusting
Rod (see Fig.27)
◎The aperture diaphragm decides the numerical
aperture of the illumination when doing bright-
field observation. If the N.A. of illumination is
matching with N.A. of the objective, you can
obtain better resolution, sharper contrast and
increase the depth of field.
●You may remove the eyepiece and observe
through the eyepiece tube directly or
insert the centering telescope when confirming
the aperture diaphragm. Adjust ring of aperture
diaphragm until seeing the view-field as Fig.27.
(Image of aperture diaphragm①, Objective
outline②)
●Usually, adjust the N.A. to its 70%~80% when
observing the dyed specimen. Adjust the aperture
diaphragm to “ ” when observing the bacteria
specimen.
15

IN480 Series
Fig. 28
4-4-6 Changing Condenser (see Fig.28)
◎When changing from low magnification
observe to high magnification observe,
condenser need to be changed to N.A.0.5
condenser. Loosen condenser fixing rod①, take
out N.A.0.3 condenser and replace with a
N.A.0.5 condenser. Tighten condenser fixing
rod.
★Please refer to 2-2-7 for matching objective
and condenser.
★To change Petri dish with N.A.0.5
condenser, rotate up condenser arm 0°～30°
and replace it.
4-4-7 Shift the condenser lens group away (see
Fig.29)
◎ Loosen tumbler lock knob② counter-clockwise
(reversal direction of “LOCK” arrow), rotate
tumbler①clockwise (counter-clockwise) to get
larger operation space. It can reach the height of
220mm.
◎After observing, rotate tumbler clockwise
until it sounds “ka-da”. Tighten the tumbler lock
knob in the direction of “LOCK” arrow.
Fig. 29
★Please note that you will not get good
illumination when moving the condenser out
of the light path. It is only for use of big Petri
dish.
16