AmScope IN480 Series User manual

IN480 Series
Inverted Biological
Microscope
INSTRUCTIONS
This manual is for the using method, troubleshooting and maintenance about
IN480 series biological microscope. Please study this manual thoroughly before
operating, and keep it with the instrument. The manufacturer reserves the rights to
the modifications. Technical specifications may be subject to changes without
notice.

Before Use
1.Components..............................................................................................................1
2.Assembling ...............................................................................................................3
2-1 Assembling Scheme........................................................................................3
2-2 Assembling Steps ................................................................................................4
3.Adjustment Device....................................................................................................8
4.Using Adjustment Device ....................................................................................... 10
4-1 Stand..................................................................................................................10
4-2 Stage..................................................................................................................11
4-3 Observing Tube.................................................................................................12
4-4 Illumination Device......................................................................................14
5.Phase-contrast observation.................................................................................... 17
5-1 Nomenclature....................................................................................................17
5-2 Assembling and operating............................................................................ 18
6.TV device & Photography device........................................................................... 20
6-1 TV device......................................................................................................20
6-2 Photography Device..................................................................................... 21
7.Technical Specifications.........................................................................................23
7-1 Technical Parameters...................................................................................23
7-2 Parameters of Objective............................................................................... 23
8.Troubleshooting...........................................................................................................24
Contents IN480 Series

1. Operation Notice
Fig. 1
Fig. 2
1. As the microscope is a high precision
instrument, always operate it with care, and
avoid physical shake during the operation.
2. Do not expose the microscope in the sun
light directly, neither in the high temperature,
damp, dust or acute shake. Make sure the
worktable is flat and horizontal.
Operating environment: temperature
5℃~40℃, maximum relative humidity 80%.
3. When moving the microscope, use both hands
to hold its stand arm ②and the front base
①, and lay it down carefully (see Fig. 1).
4. The microscope power should be grounded to
avoid lightning strike.
5. For safety, make sure the main switch③is at
“O” (off) and power it off before replacing
the bulb or fuse. Wait until the lamp cools
down.
★Bulb selected only: 6V/30W Halogen light
(Philips 5761).
6. Universal voltage is supported as 90~240V.
Additional transformer is not necessary. Make
sure the voltage is in this range.
7. Use the special cord supplied by our company.
2. Maintenance
1. Wipe the lens gently with a soft tissue. Carefully wipe off the oil marks and fingerprints
on the lens surfaces with a tissue moistened with a small amount of 3:7 mixture of
alcohol and ether or dimethylbenzene.
★As alcohol and ether is flammable, don’t place these chemical near to fire or fire
source. When turning on or off the electrical device, place these chemicals in a
ventilated place.
2. Don’t use organic solution to wipe the surfaces of the other components. Please use the
neutral detergent if necessary.
3. If the microscope is damped by liquid when using, please power it off immediately and
wipe it dry.
4. Never disassemble the microscope by yourself.
5. After using, cover the microscope with a dust cover.
Before Use IN480 Series

IN480 Series
3. Safety Sign
Sign
Signification
Hot at surface, do not touch.
Study the instructions before use. Unsuitable operation would lead to
person hurt or instrument faulty.
|
Main switch ON
O
Main switch OFF

● Stage Extension Board
● Attachable Mechanical Stage
Koehler Illuminator
Light SourceObjective
Illumination
Holder
Phase-contrast Slider
LongWorking
DistanceCondenser
Binocular
ObservingTube
Stand Nosepiece
Stage
Eyepiece
Circular Central Stage
●
Metal Stage
● Glass Stage
IN480B
1.Components IN480 Series
1

IN480 Series
Eyepiece
Trinocular
Observing Tube
● Metal Stage
● Glass Stage
● Stage Extension Board
● Attachable Mechanical Stage
Nosepiece
Stage
Stand
Circular Central Stage
Phase-contrast Slider
Illumination
Holder
Objective
Koehler Illuminator
Light Source
IN480T
LongWorking
DistanceCondenser
2

紧固螺杆
物镜
2-1 Assembling Scheme
Following is the Assembling Scheme, and the numbers denote the assembling order.
★Before assembling, make sure there is no dust or dirt. Assemble carefully and do not scrap
any part or touch the glass surface.
★Save the hex spanner. It will be used when changing the parts.
⑨
Filter
N.A.0.5 Condenser
Lamp House Cover
②
N.A.0.3 Condenser
①
Eyepiece
●
PL10×
/22
●
PL15
×
/16
⑧
Halogen Bulb
6V 30W HAL
⑦
③
⑥
Lock-screw
Glass Stage
Metal Stage
④
○
10
Power Cord
⑤
Stand
Stage Extension Board
AttachableMechanical Stage
2.Assembling IN480 Series
3

2-2 Assembling Steps
Fig. 1
2-2-1 Assemble and change the bulb (see Fig.1)
◎Lamp selected only: 6V/30W HAL bulb
(Philips 5761)
★Do not touch the bulb with finger. If there
is a fingerprint, wipe it with clean and soft
cloth.
1. Hold the bulb ①with clean glove or gauze
and insert the pins ②to the jack ②. Make
sure the bulb is vertical.
★Insert the bulb gently to avoid damage.
2. Change the bulb. The bulb will become very
hot when using or after using. Before replacing
the bulb, turn off the main power (The main
switch is at “O” and disconnect the power
cord) and wait until the lamp holder and bulb
cool down.
2-2-2 Assemble Lamp House Cover (see Fig. 2)
1. Slide down lamp house cover①by groove
until the hole②on the cover①is aligned to
the screw hole②.
2. Tighten lock screw③into screw hole②.
Fig. 3
2-2-3 Assemble the Objective (see Fig.3&Fig.4)
1. Rotate the coarse focusing knob①to lower the
nosepiece to the lowest position (see Fig.3).
★★★★ To make the instrument safe in
transit, please set he nosepieces to the lowest
position after use. Please turn the adjusting
ring②of the coarse adjustment knob to the
proper tension. (see Fig.3)
2. Screw the objectives into the microscope
nosepiece from the lowest magnification to
the highest in a clockwise direction from the
rear.
IN480 Series
Fig. 2
4

IN480 Series
Fig. 5
Fig. 6
◎For ease of use, please following this
assemble direction.
◎It can also be assembled by aperture on the
stage.
★★★★ Clean the objectives periodically
because the inverted microscope objectives are
sensitive.
★★★★ Cover the hole on the nosepiece with
a dust cap③to avoid the dust and the dirt. (see
Fig.4)
★★★★ Search and focus the sample starting
from low magnification objective (4X or 10X).
Then change to the high power magnification
ones if needed.
★★★★ After the objective replaced, rotate the
objective nosepiece until it sounds “ka-da” to
make sure the objective is in the center of light
path.
2-2-4 Assemble the Stage extension board/
Attachable mechanical stage (see Fig.5&Fig.6)
◎Stage extension board could be assembled on
both left side and right side to extend the work
stage. But the stage extension board and the
attachable mechanical stage cannot be used at
the same side.
◎Attachable mechanical stage generally is
assembled on the right.
1. Assemble stage extension board. Tighten the
lock screw①on stage extension board. Then
screw it from bottom right or bottom left.
Use the small tools to tighten till the board
is installed firmly.(see Fig.5)
2. Assemble attachable mechanical stage
Assemble it as the stage extension board (see
Fig.6).
Fig. 4
5

IN480 Series
2-2-5 Circular central stage (see Fig.7)
1. Assemble the metal stage①on the stage
aperture. Turn the metal stage V②facing to
the user. It is easier to check the objective.
2. Lay glass stage flat when using it. There is
special direction.
Fig. 7
2-2-6 Assemble the Eyepiece (see Fig.8)
1. Remove the cover of eyepiece tube①.
2. Insert the eyepiece②into the eyepiece tube
fully.
Fig. 8
2-2-7 Assemble the Condenser (see Fig.9)
1. Loosen the condenser fixing rod②until
aligned with the condenser holder③inner
wall.
2. Push condenser①into condenser holder③
as arrow direction. Make it staying
vertically.
3. Tighten condenser fixing rod②.
Fig. 9
★★ N.A.0.3condenseris used with 4X~20X
low
magnificationobjective.
★★★★ N.A.0.5 condenser is used with above
40Xhighmagnificationobjective.
★★★★ Refer to 4-4-6 to replace condenser.
6