AmScope M160 Series User manual

M160
Series
1
User’s Manual

2
M160
Series
T
abl
e
o
f
Contents
Before Use..........................................................................................3
Introduction ............................................................................................................. 3
Safety Precautions ................................................................................................... 4
Parts .............................................................................................. 5
M160 ................................................................................................................ 5
Definition of Parts ............................................................................................... 6
Getting Started ............................................................................ 7
Assembly .................................................................................................................. 7
Operation .................................................................................... 8
Settin Up ................................................................................................................... 8
Focusin ................................................................................................................... 8
Adjustin The Diaphra m .................................................................................. 8
Attachin a Camera / Chan in Eyepieces ........................................................... 9
Maintenance/Precautions ....................................................................................... 9
Specifications.............................................................................. 10
Specifications ......................................................................................................... 10
Optional Accessories ............................................................................................. 11
Objectives .......................................................................................................... 12
Eyepieces ................................................................................................................ 12
Technical Parameters ................................................................ 13
Electrical System ............................................................................................... 13
Technical Terms & Concepts .......................................................................... 14
Troubleshooting........................................................................ 15
Common Issues ..................................................................................................... 15
Common Issues (Continued) ............................................................................. 16

3
M160
Series
Before
Use
Introduction
Congratulations on the purchase of your new AmScope microscope!
This manual is designed for the M160 series microscopes.
Please be sure to take a few minutes to familiarize yourself with the features and functions of your
new AmScope microscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
www.iScopeCorp.com
We highly recommend you study this manual thoroughly before operating the microscope, and
that you keep it on hand for future reference.
If you have additional questions or need assistance, please send us an email at:
[e-mail]

4
M160
Series
Before
Use
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transportation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry
and clean place with temperatures between 32-100 degrees °F (0-40 degrees °C), and in
maximum relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consistent with
the voltage of your microscope.

5
M160
Series
Parts
M160-LED
E
yepiece
Monocular Head
Nosepiece Turret
Objectives
Stage
Base Light
Stage Clips
Coarse
Focusing nob
Dimmer
Power
Switch
Power
Socket
Top Light
Head Lock Screw
Fine
Focusing nob

6
M160
Series
Parts
Definition of Parts
BaseIlluminatorLens
Directs the li ht source towards the slide
FocusingKnob
Used to brin the slide into the si ht and focus
Disc Iris Diaphragm
Controls the amount of li ht that hits the slide from the base illuminator
Dimmer
Controls the amount of li ht that escapes from the base lens
Nosepiece
Houses the objective lenses
RotatingMonocular Head
Houses the eyepiece and optics of the microscope

7
M160
Series
Getting
Started
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Monocular Microscope Body & Head
- Three Achromatic Objectives (4x, 10x, 40x)
- 10x Widefield Eyepieces
- 25x Widefield Eyepieces (for -C models only)
- One Dust Cover
- 110V AC power adapter and 3 AA batteries
Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing.
3. Remove the microscope body from the box and remove the plastic protective covering. The body of the micro-
scope is composed of the base, the stage, the arm, and the nosepiece.
4. Remove the eyepiece cover and place the 10X eyepiece into the tube.
5. Plug in the microscope and turn it on. Power switch is at the back of stand. Light dimmer is the knob
behind the base light. If no light emerges from the light source, adjust the dimmer knob.
Power Switch Position and function:
I: Base Light
O: Light OFF
II: Top Light
6. To use battery power, install 3 AAA batteries at the bottom.

8
M160
Series
Operation
Setting Up
1. The observation head is 360 degree ratable. It is easy to turn the head to a comfortable position. Loosen the
head lock screw before turning the head.
2. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.
3. Center the specimen over the stage opening, lining it up with the light and the objective lens.
4. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of
light is achieved. Power switch has three positions: I is for base light; O is off; II is for top light.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to
locate and focus on the specimen. As you move up in magnification you may need to refocus the image a little
each time.
2. Being focusing by first looking with one eye through the eyepiece. Close your other eye. Use the focusing knob
to adjust the height of the stage until the sample comes into clear focus.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if moving the
stage very close to the objectives to prevent slide or objective damage.
Adjusting the Diaphragm
By changing the aperture (hole size) of the iris diaphragm, you can
adjust the background brightness. Adjust the aperture of the iris
diaphragm by rotating the disc underneath the stage to the desired
aperture.
Iris Diaphragm
Disc

9
M160
Series
Operation
Attaching a Camera / Changing Eyepieces
Remove the 10x eyepiece, you can change another eyepiece or drop in the camera.
Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a can of com-
pressed air or gently wiped off with a soft lens paper tissue/nonabrasive lint free cloth.
- Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).
- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.
- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.
- After use, cover the microscope with the provided dust cover.
- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damage.
- To change batteries with this unit, place 3 AA batteries in the case at the bottom of the base.
- The unit can be used as a recharger for AA batteries specified as rechargeable only. Please do not use standard
batteries in the unit with the charger plugged in to avoid damage.

M160
Series
Specifications
10
Parts Specifications M160 M160C
WF Eyepiece WF10X/18mm
WF10X/18mm w/ Pointer xx
WF10X/18mm w/ Reticle
WF16X/13mm
WF20X/10mm
WF25X10mm x
Plan Eyepiece P5X
P10X
P16X
DIN Achromatic Objectives 4X/0.10 xx
10X/0.25 xx
40X(spring)/0.65 xx
60X(spring)/0.85
100X(spring,oil)/1.25
Plan Objectives 4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head Binocular Sliding,360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
Monocular, 360 Degree Swiveling xx
Diaphragm Iris Diaphragm Disc xx
Illumination Halogen Light w/ Dimmer
LED Light w/ Dimmer xx
Lamp 6V/20W
6V/30W
LED xx
Filter Blue/Yellow/Green

M160
Series
Specifications
11
Optional Accessories
Parts Description Model # Purpose
Eyepiece 5X EP5X23 Obtaining 20x, 50x, 200x, and 500x magnification
powers
20x EP20X23 Obtaining 80x, 200x, 800x, and 2000x magnifica-
tion powers
25x EP25X23 For obtaining 250x and 2500x magnification pow-
ers
10x w/ Pointer EP10X23P For easier identifying of objects
10x w/ Reticle EP10X23R For measuring objects
Objective 2X A2X For obtaining 20x and 32x magnification powers
5X A5X For obtaining 50X and 80X magnification powers
20X A20x For obtaining 200x and 320x magnification powers
60X A60X For obtaining 600x and 960x magnification powers
Plan 4X PA4X For obtaining higher clarify in images
Plan 10X PA10X For obtaining higher clarify in images
Plan 40X PA40X For obtaining higher clarify in images
Plan100X PA100X For obtaining higher clarify in images
Camera CMOSDigital MU035 (350k)
MU130 (1.3mp)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
To capture images, video, or view live display on a
computer (PC/Mac OS X)
Calibration Micrometer MR400 To calibrate the camera software for on screen
measurements
CCD TV/Video (Trinocular
Only)
CCD-NP To view live display on a television (RCA)
Case Aluminum Case AC-B100 For carrying microscope around safely

M160
Series
Specifications
12
Objectives
Type Magnification Numerical Aperture
(N.A.)
Medium Parfocal Distance (mm) Magnification Marks
(Color Ring)
DIN Achromatic
Objective (195mm)
4X A4X / .10 Air 45 Red
10X A10X / 0.25 Air 45 Yellow
40X A40X / 0.65 Air 45 LightBlue
60X A100X / 1.25 Air 45 Deep Blue
100X PL4X / .10 Cedar Oil 45 White
Plan Objective
(195mm)
Plan 4X PL10X / 0.25 Air 45 Red
Plan 10X PL40X / 0.65 Air 45 Yellow
Plan 40X PL100X/1.25 Air 45 LightBlue
Plan 100X A4X / .10 Cedar Oil 45 White
Eyepieces
Type Widefield Eyepiece
Medium
Plan Eyepiece
Magnification 10X 15X 20X 5X 10X 16X
Field of View
Φ 18 Φ 13 Φ 11 Φ 18 Φ 18 Φ 15

13
M160
Series
T
echnica
l
Parameters
Electrical System
There are two options for electrical systems for this series of microscope. The light source is an LED system.
1. 220V~240V power supply: 220V~240V ±10%, 50Hz
This electrical system is CE and GS certified.
2. 100V~120V power supply: 100V~120V ±10%, 60Hz
This electrical system is UL certified.
All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent
on which unit is purchased.
This unit is also capable of using batteries and charging rechargeable batteries. It uses 3 AAA batteries.
When plugged in with batteries in the unit, the batteries will be charged.
Note: Please do not use the recharging feature with standard batteries, as damage to your unit can occur.

14
M160
Series
T
echnica
l
Parameters
Technical Terms & Concepts
TotalMagnification
Total magnification of a microscope is calculated by the magnification of the objective multiplied by the
magnification of the eyepieces.
-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification
Field of View
Linear field of view of the eyepiece divided by the magnification of the objective
Numerical Aperture (N.A)
Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the
features of the objective and condenser’s image quality and resolution. The “n” is a refractive index of the
medium (air or immersion cedar oil) between the objective lens and the specimen. The “ α ” is 1/2 of the angle
between the aperture on the objective and path of light. The larger the N.A, the higher the resolution of the
objective (and better quality of the image) is.
Object to Primary Image Distance
The distance between the object plane and the primary image plane. The conjugate distance is fixed.
Mechanical Tube Length
The distance between the objective shoulder and the ocular shoulder

15
M160
Series
T
roubleshooting
Common Issues
Symptom Cause Remedy
OPTICAL ISSUES
One side of the field
of view is darker
The nosepiece if misaligned Turn the nosepiece until it clicks into place
Stains or dust has accumulated on the objective,
eyepieces, or base lens
Clean all lenses with lens cleaner or a lint free non-
abrasive cloth
Obstructions are
observed in the field
of view
Stains, dust, or dirt has accumulated on the speci-
men
Clean the slide or use a new specimen if sample is
destroyed
Stains, dust, or dirt have accumulated on the lens Clean the lens
UnclearImage There is no cover slip on the slide Add a cover slip. The objectives are designed for use
with a 0.17mm cover slip, so it is a requirement to
use one for proper images.
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm
thickness slip
The aperture is not open to an appropriate diam-
eter
Adjust the aperture to have the light just larger than
the size of the condenser
Stain or dust has accumulated on the lens in the
inlet of the head
Clean the lens with lens cleaner or a nonabrasive
lint free cloth, as well as spray with compressed air
One side of the field
of view is dark or the
image moves while
focusing
The specimen slide is not fixed Secure the slide to the stage with clips
The nosepiece is not in the right position Turn the nosepiece until it clicks into place
The field of view is not
bright enough
The iris disc diaphragm is not big enough Rotate the iris disc diaphragm to allow more light
to travel
Stains, dust, or dirt has accumulated on the con-
denser, objective, eyepieces, or base lens
Thoroughly clean tall lenses with lens cleaner or a
lint free nonabrasive cloth

16
M160
Series
T
roubleshooting
Common Issues (Continued)
Symptom Cause Remedy
OPTICAL ISSUES
The color of the image is
notaccurate
The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color
clarity
No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired
filter
MECHANICALISSUES
The objective touches the
cover slip
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip
The limit-stop is set too high or not engaged Be careful to avoid contact between objective and the slide when
the limit stop is not engaged. To reengage, focus the sample, then
lock the limit stop into place to set max height at a safe but us-
able distance.
Unable to move the slide
smoothly
The slide is not secured correctly Adjust the slide to use the stage clips and secure the sample
The mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage
Focus knob does not turn The tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus
knob counterclockwise (close to the arm of the microscope on
the left of the microscope)
Stage declines by itself The tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus
knob clockwise (close to the arm of the microscope on the left
of the microscope)
The focusing knob won’t
raise the stage
Limit-stop is engaged Disengage the limit stop on the rear of the stage of the micro-
scope
ELECTRICAL ISSUES
The bulb/lightsource
flickers
The bulb is close to burning out Please contact us in regards to this issue. LED lights do not burn
out, so there may be another electrical issue
The microscope does not
light up
The microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina-
tion