AmScope B100 Technical manual

15
B100/B200
Series Biological Microscope
Operation Instruction
To ensure the safety and obtain satisfactory performance, please study
this operation instruction thoroughly before your operation.

16
Contents
1.Purpose……………………………………………………………………………2
2.Specifications………………………………………………………………………2
3.Installation…………………………………………………………………………4
4.Operation……………………………………………………………………………4
5.Maintenance…………………………………………………………………………8
6.Troubleshooting……………………………………………………………………10
7.Outfits……………………………………………………………………………13

1
Seidentopf Binocular Head
QuadplexNosepiece
Objective
Clamp
Condenser Up- downKnob
Coaxial CoarseAnd
ElectricBase
Eyepiece
MainBody
Stage
StageMovingKonb
Condenser
Handleof IrisAperture Power SwitchAnd
Light Collector
Fig.1 B200Binocular Biological Microscope
FineFocusingKnob Light Controller
Condenser Up- downKnob
Coaxial CoarseAnd
ElectricBase
Eyepiece
Nosepiece
Objective
FineFocusingKnob
Monocular Head
MainBody
Clamp
Stage
Condenser
Light Controller
Power SwitchAnd
Light Controller
Fig.2 B100Monocular Biological Microscope

2
1. Purpose
B series biological microscope is designed for teaching, clinical verify. The series
microscope with original style, steady structure, convenient operation and clear
image is suitable for observing various biological specimens, and mostly applied in
school, college, hospital and family.
2. Specifications
2.1 Total magnifications
Objective
Total Magnifications
Eyepiece
4X
10X
40X
100X
10X
40X
100X
400X
1000X
16X
64X
160X
640X
1600X
2.2 Objectives
Objective
Numerical
Aperture
(N.A.)
Objective Field
Resolving
Power
Working Distance
Field
Number
ф18
Field
Number
ф20
Achromatic
Objective
Plan
Objective
4X
0.10
4.5mm
5mm
2.8µm
13mm
16mm
10X
0.25
1.8mm
2mm
1.1µm
6.3mm
2mm
40X (S)
0.65
0.45mm
0.5mm
0.42µm
0.44mm
0.66mm
100X
(Oil) (S)
1.25
0.18mm
0.2mm
0.22µm
0.3mm
0.42mm

3
2.3 The other specification
2.3.1 Mechanical tube length: 160mm
2.3.2 Conjugate distance: 195mm
2.3.3 Head: Monocular, Inclined 30°, Rotable 360°, Eyepiece can be fixed,
Anti-fungal systems.
Seidentopf binocular head 30°, Rotable 360°,
Interpupillary adjustable distance is 55-75mm, Diopter adjustable range
±5, Anti-fungal systems.
2.3.4 Nosepiece: Quadplex nosepiece
2.3.5 Mechanical stage: Size 142mm×134mm
X-Y travel 50mm×70mm
2.3.6 Focusing systems: Coaxial coarse and fine focusing knobs, Coarse stroke
22mm,
Fine division 2µm, Condenser up-down range 25mm
2.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture,
Aperture center is adjustable.
2.3.8 Mirror: Mirror diameterф52mm (optional)
2.3.9 Filter: Built in blue filer, ф38.5mm
2.3.10 Anti-fungal systems: Eyepiece and objective is anti-fungal, anti-fungal system
built in head.
2.3.11 Electric components: Input voltageAC85-265V, 50/60Hz
Output voltage DC1.2-6V
6V/20W halogen lamp

4
Rotation potentiometer with power switch
Fuse 2A ф5×20
3. Installation
B series microscope is packed integrate, and it is convenient for installation.
Open the package carton, and insert eyepiece into the eyepiece tube of the head
(eyepiece for monocular microscope has been inserted before packing). If objectives
don’t be fixed, please take objectives out from objectives packing box, and drive
them into the holes of nosepiece orderly and tightly according to the times.
Take out the oil bottle and dust cover, and place it properly for convenient use.
4. Operation
4.1 Electric components
Make sure the supply voltage meets the instrument’s requirement, then plug in it.
Rotation potentiometer with power switch used by the instrument is for protecting
lamp.
Mirror
Bracket
Mirror Base
Fig.4 Mirror
ClampHandleSpecimen Clamp Condenser
Vertical
MovingKnob
Horizontal
MovingKnob
Handleof
IrisAperture
Condenser
Up- downKnob
Fig.3 StageAndCondenser

5
4.2 Placing specimen (Shown in Fig.3)
Please place specimen on the stage, and the face with cover glass should be up, then
clip it with clamp. Clipping specimen should be careful in order to avoid spoiling
glass. Place specimen on the stage levelly, and make it into optical path by adjusting
vertical moving knob and horizontal moving knob.
4.3 Condenser (Shown in Fig.3)
4.3.1 Condenser up-down
Turn the condenser up-down knob to adjust the distance between condenser front and
the specimen, and change the equal illumination to obtain the best brightness.
4.3.2 Iris aperture adjustment
Stir the handle of iris aperture of condenser to
adjust size of iris aperture for adjusting the
contrast of the specimen.
4.4 Interpupillary distance and diopter
adjustment (Shown in Fig.5)
4.4.1 Interpupillar distance is different for everyone, so interpupillary distance should
be adjusted before using binocular microscope. Please take the two eyepiece tubes to
turn until the bright rings observed by two eyes are in superposition entirely.
▲B100 monocular microscope don’t need to be adjusted.
4.4.2 Diopter adjustment
As focusing for binocular, user should observe right eyepiece with right eye, and
make the right eyepiece clear by focusing adjustment, then observe the left eyepiece,
at the same time, adjust the diopter ring of the left eyepiece tube to make the image of
Diopter Ring
Eyepiece
Interpupillary
DistanceScale
Fig.5 InterpupillaryDistance
Adjustment And Diopter Ring
EyepieceTube

6
left eyepiece clear as same as the right eyepiece.
4.5 Focusing adjustment
Usually 10X objective should be used for focusing adjustment first. Take 10X
objective into bright path, then observe the specimen with right eyepiece by right eye
and turn coarse focusing knob to find the image, then turn slowly fine focusing knob
to make the image clear.
All objectives of our microscope are precisely adjusted, and approximate clear image
should be found with the other objectives after getting the clear image with 10X
objective. If the image isn’t enough clear with the other objectives, please turn fine
focusing knob slightly to find the satisfactory image.
▲Don’t turn left and right coarse and fine focusing knobs with different direction with
power at the same time, if so, the focusing system will be damaged.
▲Don’t directly pull objective to turn nosepiece when user changes the different
objectives, if so, optical quality of
microscope possibly be affected. The
right way is to take the tooth-like part
of the nosepiece to turn it, and make
the objective into correct position and
into the bright path.
4.6 Usage of the tension adjustment
ring (Shown in Fig.6)
The tension adjustment ring can adjust the tension of the coarse and fine focusing
unit to prevent the stage from sliding down automatically and to improve the comfort
Fig.6 Coarse A F Fnd ine ocusing
Left Coarse
Left Fine
Right Fine
Right Coarse
Tension
FocusingKnob
FocusingKnob
FocusingKnob
Adjustment Ring
FocusingKnob
KnobsAnd Tension Adjustment Ring

7
of operation. Rotation clockwise makes tension decrease, and by contraries, rotation
counterclockwise makes tension increase.
4.7 Usage of immersion oil objective
The 100X objective of B series microscope can be used for observation even if
without immersion oil. However, adding moderate immersion oil between the front
lens of 100X objective and the cover glass of specimen can make the image clearer.
Please pay attention that air bubble and impurity can’t be in the immersion oil,
otherwise, the image would be affected.
First of all, take 40X objective which has been focused well out from bright path,
then take 100X objective into bright path.At this time, nosepiece or stage moving
knob should be turned slightly, and also slightly turn fine focusing knob to clear away
the air bubble of immersion oil, otherwise, the air bubble would affect the image
badly.
▲After immersion oil used, the oil of specimen and the microscope surface should be
soon cleaned by absorbent cotton, lens paper, gauze or soft cotton cloth with
moderate mixture of pure industrial alcohol and ether (proportion 1:4)
▲Standard thickness 0.17mm cover glass should be chosen when high times objective
used, and thickness error should be within 0.01mm, otherwise, image definition
would be affected.
4.8 Mirror (Shown in Fig.4)
Mirror of B series microscope is optional, and it is used when electrical power isn’t
supplied.
Mirror doesn’t be suggested as illumination source when 100X objective used.

8
Take light collector down from base, then take mirror on base. Turn mirror to make
reflecting face to the light source, and make reflecting face and horizontal face as 45°
approximately to make the light to specimen as possible.The mirror has two sides, one is
a plane mirror, and the other one is a concave one. Concave mirror is suitable for
large-area outside light source, such as bright sky.
4.9 Points of attention after using
4.9.1 Power of microscope should be turned off after using, and the plug should be
pulled down. If immersion oil used, please clean objective and specimen soon.
Finally, cover microscope with dust cover.
4.9.2 Please take eyepiece and objectives out from microscope if user will stop using
it for a long time, and place eyepiece and objectives into drier with drying agent.
Cover microscope with dust cover.
5.Maintenance
5.1 Clean microscope
5.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or
absorbent cotton or cleaned by absorbent cotton, lens paper with the mixture of
alcohol and ether (proportion 1:4).
5.1.2 Alcohol and ether all are burnt easily, please take them away from fire. Be
careful for turn on and off power.
5.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such as
alcohol, ether or the mixture of the both. Silicon cloth or soft cleaning preparation is
suggested to clean it.

9
5.1.4 Plastic should be cleaned by soft cloth with clear water.
5.2 Environment of using and placing
5.2.1 Microscope should be used and placed in a cool, dry, non-dust, non-shake and
non-corrosive gases environment.
5.2.2 Microscope should be used in environment of indoor temperature 0°-40°C and
maximum relative humidity 85%.
5.2.3 Removing humidity equipment is suggested to be installed when microscope
used in heavy humidity area to avoid fungus and mist damage instrument.
5.2.4 Please pay attention to prevent microscope from violent shake and vibration in
application and in carrying. Don’t drag it on the surface of worktable to avoid
damage microscope and worktable.
U screwKnurledn
LampAdjustment Knurled
Pull Over
Bulb
HousingCover,
ReplaceBulb,
CloseCover
TheLamp
Cover
ThumbScrew
ThumbScrew
Fig.7 Replacement of Bulb
5.3 Replacement of bulb (Shown in Fig.7)
5.3.1 Turn off power, and pull out plug.
5.3.2 Wait the bulb become cool.
▲Please be sure that the bulb is cool, then follow by the operations.
5.3.3 Lay aside the microscope reliably, unscrew the knurled thumb screw of the

10
lamp housing cover on the underside of base.
5.3.4 Pull over the lamp housing cover.
5.3.5 Pull out the bulb should be replaced, hold a new bulb with silk cloth to avoid
fingerprint and dust affect bulb brightness and service life, and insert fully the contact
pins into the bulb socket.
5.3.6 Close the lamp housing
cover, and screw the knurled
thumb screw.
▲After working for above 10
hours continuously, better cut off
the microscope about 30 minutes.
5.4 Replacement of fuse (Shown in Fig.8)
5.4.1 Cut off power of microscope, and pull out the plug.
5.4.2 Unscrew fuse cap in the back of base, take out old fuse.
5.4.3 Replace a new fuse, then screw the fuse cap.
5.5 Stop to use microscope, please cut off power, cover the dust cover, and place it in
a cool and dry environment.
6.Troubleshooting
In the period of using B series microscope, if there is any trouble occurs, please
referring to the following sheet listing some common troubleshooting，and resolve
them.
Power Wire FuseCap
Fig.8 Replacement of Fuse

11
Trouble
Causation
Remedy
Switch on but bulb dark
Plug is unreliable
Plug in again
Bulb is broken
Change bulb
Fuse is broken
Change fuse
Bulb is flickering or
brightness is unsteady
Bulb is unstable
Insert it again
Bulb is broken
Replacing bulb
Brightness of view field
isn’t enough or is
uneven
Bulb specification doesn’t
meet the requirement
Replacing bulb
Brightness isn’t adjusted
correctly
Adjust rotation
potentiometer
Objective isn’t in correct
position
Make the objective in
correct position
The size of iris aperture is too
small
Adjust the size of iris
aperture
Lens (objective, eyepiece,
condenser, light collector) has
dust
Clean it
Position of condenser is too
low
Higher condenser
Image isn’t clear
Cover glass of specimen
doesn’t meet the requirement
Use required thickness
cover glass (0.17mm)
Cover glass of specimen isn’t
in up direction
Place specimen correctly

12
(contrast or definition
isn’t enough)
Surface of objective lens is
dirty (especially it is easy for
the front lens of 40X
objective to dip in immersion
oil)
Clean it
Immersion oil isn’t used for
100X objective (oil)
Use immersion oil
Immersion oil doesn’t meet
the requirement
Use immersion oil
supplied by us
There is bubble in immersion
oil
Clear the bubble way
Size of iris aperture isn’t
proper
Adjust the size of iris
aperture
Position of condenser is too
low
Readjust the position of
condenser
One side of image is
dark or image is moving
as focusing
Objective isn’t in correct
position
Make the objective in
correct position
Specimen isn’t placed
correctly
Place specimen levelly
on stage and clip it with
clamp
Objective touches
specimen as changing
low times objective to
high times objective
Cover glass of specimen isn’t
in up direction
Place specimen correctly
Cover glass doesn’t meet the
requirement
Use required thickness
cover glass (0.17mm)
Image observed by two
eyes aren’t in
superposition entirely.
Interpupilary distance isn’t
adjusted correctly
Adjust interpupilary
distance according to two
eyes
It is easy for eyes to be
tired during observing
Diopter isn’t adjusted
correctly
Readjust diopter

13
B Series Biological Microscope Outfits
Items
Specification
B102
B103
B104
B202
B203
B204
Order No.
Eyepiece
WF 10X-18mm
●
●
●
●●
●●
●●
WF10
WF 16X-13mm
○
○
○
○○
○○
○○
WF16
WF 10X-18mm
(With Pointer)
○
○
○
○
○
○
WF10P
WF10X-18 mm
(Reticule 0.1 mm)
○
○
○
○
○
○
WF10R
WF 5X-20 mm
○
○
○
○○
○○
○○
WF5
Achromatic
Objective
4X/0.10
●
●
●
●
●
B-A4
10X/0.25
●
●
●
●
●
B-A10
40X/0.65 (S)
●
●
●
●
●
B-A40
100X/1.25(Oil)(S)
○
○
●
○
●
B-AO100
Plan
Objective
4X/0.10
○
○
○
○
●
B-P4
10X/0.25
○
○
○
○
●
B-P10
40X/0.65 (S)
○
○
○
○
●
B-P40
100X/1.25 (Oil) (S)
○
○
○
○
●
B-PO100
Monocular
Head
Inclined 30°,
Rotatable 360°
●
●
●
Mo-1
Seidentopf
Binocular
Head
Inclined 30°,
Rotatable 360°,
Interpupilary Distance:
55-75mm
●
●
●
Bi-1
Nosepiece
Quadplex,
Coaxial Coarse and
Fine Focusing Knobs
●
●
●
●
●
●
N-4
Fixed Stage
Clips
●
●
○
S-1
Attachable
Mechanical Stage
Travel:
30mm×50mm
○
○
●
○
○
S-2
Mechanical
Stage
Travel:
50mm×70mm
●
○
●
●
S-Ma

14
Condenser
Single Condenser
(5 Aperture Disc
Diaphragm)
●
○
CS
Abbe N.A. 1.25
(Iris Diaphragm)
●
●
●
●
●
Ca
Dark Field
Ring
Available For
4X-40X Objective
○
○
○
○
○
D-1
Base
Mirror
Built In Blue Filter
○
○
○
○
○
○
Mo
Incandescent Lamp
110V/220V, 20W
Spare Lamp:1pc
Built In Blue Filter
●
○
E1
Halogen Lamp
6V 20W
Wide Voltage:
85V-265V
Spare Lamp: 1 pc
Built In Blue Filter
●
●
●
●
●
E2
LED Illumination
Systems
○
○
○
○
○
○
LED
Fuse
Spare Fuse: 2 pcs
●
●
●
●
●
●
Note: “●”In Table Is Standard Attachment. “○” Is Optional Accessories
Packing Size: 340mm X 243mm X 425mm
Gross Weight: 7 kgs Net Weight: 5.5 kgs