aplegen Omega Fluor User manual

Omega Fluor™and
Omega Fluor Plus™
Gel Documentation Systems
User Manual

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Table of Contents
Section Page
Omega Fluor
Introduction 3
Components and Parts 3
Omega Fluor System Specications 4
System Placement 5
Setup Illustrations 5
Software Installation 6
Omega Fluor Plus
Introduction 12
Components and Parts 12
Omega Fluor Plus System Specications 13
System Placement 14
Setup Illustrations 14
Omega Fluor and Omega Fluor Plus
Taking an Image 16
Omega Fluor
Taking an Image on the Omega Fluor 17
Selecting Your Light Source 17
Capturing Your Image 18
Omega Fluor Plus
Taking an Image on the Omega Fluor Plus 20
Selecting Your Light Source 20
Capturing Your Image 22
Omega Fluor and Omega Fluor Plus
Overview of Software Features 23
Omega Fluor UV Cutoff Switch 27
Common Imaging Questions 28
Appendix A—Ordering Information 29
Appendix B—Routine Maintenance 30
Appendix C—Regulatory 33
Appendix D—Mitsubishi Electric P-95D Printer Driver Installation 36
Omega Fluor
Appendix E—System Requirements 39
Omega Fluor Plus
Appendix F—Installing or Changing Emission Filters 40
Appendix G—USB Installation Guide 41
Appendix H—Basic Tablet Operations 43

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Introduction
Congratulations on purchasing your Aplegen Omega Fluor Gel Documentation System. The Omega Fluor is a powerful
yet simple tool for gel documentation and generating publication quality, 16bit tiff images. The Omega Fluor comes
completely assembled for quick startup. It includes Omega Fluor Acquisition Software which is compatible with Microsoft
Windows XP and Vista, Windows 7 (32 and 64 bit), and Windows 8 (32bit only). It has a clean user interface and
simple tools for annotations and contrast adjustments. For more in depth analysis, the data can be exported to a gel
analysis software.
Components and Parts
The Omega Fluor system includes the following
components:
• The cabinet—The cabinet is a light tight
imaging station. It includes a 302nm UV
transilluminator on a pull out tray. Samples
can be seen directly through the UV protected
viewport. The viewport includes an orange lter
for improved visual contrast. The cabinet also
contains an EPI white light.
• The camera—The Omega Fluor camera is
a 5MP and is pre-installed in the cabinet. The
590/50nm orange lter is pre-assembled on the
end of the lens
• The software—The Omega Fluor Acquisition
Software is included on the USB Drive and
can be loaded on any Windows™ compatible
computer.
Omega Fluor

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Specications
Camera •5MP for high resolution images
• USB connection for simple set up
• Fast refresh rate for live imaging
• High quality images ideal for downstream analysis
Lens 8mm F1.4 Lens
Field of View 20 cm by 24 cm
Cabinet •302 nm pull out UV transilluminator (or optional 365 nm)
•UV viewing port
•EPI white lights
Emission Filter 590 nm
Applications •Fluorescent gel imaging
•Visible gel imaging
•Gel documentation
Certications CE; CSA; UL compliant
Product Footprint 34.6cm x 31.1cm x 68.6cm
Computer Requirements Desktop Computers
•Supported OS:Windows 8 32 bit;Windows 7/Vista 32 or 64 bit,Windows XP
(with Microsoft .NET Framework 3.5)
• Minimum hardware requirements: 1.4 GHz processor speed, 2GB RAM, 16 GB
Free Hard Disk Space, 2 USB (camera and printer)
Laptop Computers
• Supported OS: Windows 8 32 bit; Windows 7/Vista 32 or 64 bit, Windows XP
(with Microsoft .NET Framework 3.5)
• Minimum hardware requirements: 1.4 GHz processor speed, 2GB RAM, 16 GB
Free Hard Disk Space, 2 USB (camera and printer)
Omega Fluor System Specications
Omega Fluor

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Setup Illustrations
• Begin by placing the Omega Fluor on a at, level
surface with adequate clearance on all sides.
• Plug the USB camera connection into the
computer.
• Connect the power supply and the AC adapter
cord.
• Plug the cord into a power outlet.
• Aplegen recommends a surge
protecting powerstrip to protect
against potential damage from power
surges.
• Install the Omega Fluor Software onto the
computer which will run the instrument.
Omega Fluor
System Placement
As with all electrical instruments, the Omega Fluor imaging system should be located away from water, solvents, or
corrosive materials, on a at and stable surface with adequate clearance on all sides. The top of the system should
have at least 10 cm clearance to allow sufcient air ow around the camera head.
The system is intended for indoor use with the following ambient conditions:
a. Altitude up to 2000 m;
b. Temperature 5 °C to 40 °C;
c. Maximum relative humidity 80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at
40 °C;
Further, the system should be placed away from interfering electrical signals and magnetic elds. If possible, a dedicated
electrical outlet should be used to eliminate electrical interference from other instrumentation in your laboratory.