ATAGO RX-7000a Manual

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Standard Operating
Procedure （SOP）
Automatic digital
Refractometer
RX-7000α(alpha)
Preface
To ensure proper care operation, many years accurate measurement, the Standard Operating
Procedure (SOP) document for the automatic digital refractometer,RX-7000α, is provided
herewith. The following instructions should be used as an example. Based on the steps
provided, write up your company’s own original SOP for the operation of the automatic
digital refractometer, RX-7000α.
The standard operation procedure document for the automatic
digital refractometer, RX-7000α
1. Operation Manual
Plug the AC power supply cable into an AC100 to 240V power outlet. If the optional
digital printer for the RX-7000αis connected, turn on the power supply switch of the
printer first, and then, turn on the power supply switch of the RX-7000α.
1) After checking the initial screen (opening message) and also checking the present
temperature in the measurement display screen, perform zero adjustment with
distilled water.
2) Drop 0.1ml or more distilled water onto the prism surface and close the cover lid,
then, press the SW1 (ZERO) key. Screen displays “WAITING…”. After the current
temperature(PRESENT) reaches the targeted temperature (TARGET), the screen
displays ”READING…”. After a few seconds, screen displays “ZERO SET END”.
Confirm the display of "ZERO SET END".
Wipe off the distilled water from the prism surface with tissue paper.
3) Drop 0.1ml or more of sample onto the prism surface and close the cover lid, then,
press the START key. Screen displays “WAITING…”. After the current temperature
(PRESENT) reaches the targeted temperature (TARGET), the screen displays
“READING…”. After a few seconds, the measured value will be indicated.
4) Record the measured value. If the printer is connected, it will print the
measurements taken. Wipe off the sample from the prism surface with tissue paper.
5) Measure the next sample.
6) After measurement is completed, wipe off the sample from the prism surface and the
surrounding area with soft tissue paper soaked in water and then wipe dry.
7) Turn off the power supply switch of the RX-7000α.If the printer is connected, turn off
the power supply switch of the printer.
*Keep the "instruction manual" of the RX-7000α in a convenient place for easy reference.

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Standard Operating
Procedure （SOP）
Automatic digital
Refractometer
RX-7000α(alpha)
2. Daily Check
Turn on the power supply switch. After checking the initial screen (opening message) and
also checking the present temperature in the measurement display screen, perform zero
adjustment with distilled water.The RX-7000α can be used by performing zero adjustment
with distilled water as a daily check. (Although, it is not necessary to perform zero
adjustment after each measurement. It is recommended to perform reference point
adjustment again when the refractometer has not been used for an interval of more than one
hour.)
*Zero adjustment is the operation to confirm the standard point (zero point) of the refractive
index in the RX-7000α. If zero point is confirmed by the calculation software of the
refractometer, that is all for calibrating all measuring spans.
3. Periodical Inspection
Inspect the RX-7000α with standard sucrose solution once a month to see if it indicates the
measured value correctly.
Select a concentration point which you often measure and use this as a reference for
inspection.
Save the checked data and record.
3-1. Inspection by Brix (standard sucrose solution)
Make a standard sucrose solution of 10.00%, 30.00%, and 50.00% correctly, and measure
them. Confirm that the measured value coincides with each standard sucrose solution.
(The method of making the standard sucrose solution is written in [data 1] of the
introduction for validation.).
Record of Periodical inspection
Distilled water
(0.00%)
Brix 10.00%
Brix 30.00%
Brix 50.00%
1st measurement
2nd measurement
3rd measurement
Caution
Measure at room temperature of 18.0 to 22.0
℃
.
Be sure to set the targeted temperature of the RX-7000α to 20.00℃.

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Standard Operating
Procedure （SOP）
Automatic digital
Refractometer
RX-7000α(alpha)
3-2. Inspection by nD (refractive index)
The RX-7000α has the thermostatic function.
Measure distilled water by setting the targeted temperature to 10.0, 20.0, 30.0, 40.0,
50.0, 60.0, and 70℃respectively.
Be sure to perform zero adjustment with distilled water at each temperature. Then,
measure distilled water. Confirm that the measured value coincides with "each
temperature of the refractive index of distilled water".
Accuracy :
Under measurement temp. 10.0 to 30℃
±0.0002
Under measurement temp. 31.0 to 70℃
±0.0003
4. Calibration
1) Perform the calibration once a year.
2) Perform in accordance with the performance test method of operational qualification (OQ).
3) Record the data and other items in a performance test report, and have an operator
and approver’s signature. The manufacturer of the refractometer may also be
requested to perform calibration.
5. Maintenance
1) When measurement is completed, be sure that nothing on the prism surface and
surrounding areas are completely wiped off with tissue paper moistened with water,
then wipe dry with tissue.
2) When the body of the instrument is dirt y, wipe it with dry tissue, cloth, etc.
3) If the digital printer is used, the thermo sensitive roll paper is a consumable item.
4) Place the refractometer at a designated location. Keep it in a place of relative humidity
less than 75%, if it is not to be used for a long period of time.
Temperature
Refractive index
10.0
℃
1.33369
20.0
℃
1.33299
30.0
℃
1.33194
40.0
℃
1.33061
50.0
℃
1.32904
60.0
℃
1.32725
70.0
℃
1.32533
When calibrating the RX-7000α at your company (end user), it is required to meet the
difficult conditions (maintenance and check for environmental conditions, examination
conditions and the accuracy of standard liquid, and the traceability of the jigs used) for
calibration.
In order to calibrate the RX-7000α, enormous expense and time may be spent.
When the matter of expense is taken into consideration, it may be more benefical to
request the manufacturer of the refractometer to carry out calibration.
* In that case, contact the company where the refractometer was purchased.

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Standard Operating
Procedure （SOP）
Automatic digital
Refractometer
RX-7000α(alpha)
6. In the event of a malfunction (failure)
When operational malfunction (abnormalities) of the RX-7000α occurs, check the operation of
the RX-7000α by the following procedure.
After turning on the RX-7000α, check the technical malfunction by "2. Daily check" and
measurement of the solution of a known concentration. (example: Brix 10%)
6-1. Check Procedure
1) Turn off the power supply switch of the RX-7000α and turn on again.
2) Confirm the initial screen (opening message) and the present temperature in the
measurement display screen.
3) Perform zero adjustment with distilled water.
4) Measure the solution of a known concentration. (example: Brix 10%)
In the above procedure, operational malfunction (abnormalities) can be checked or found.
Properly identify the situations of operational malfunction (abnormalities) of the
RX-7000α correctly.
6-2. Proper care
Depending on the operational malfunction (abnormalities) of the RX-7000α, decide proper
care to correct.
The cause of operational malfunction (abnormalities) can be divided roughly into two areas.
1) The measured value is lower or higher due to an error in operation, mistake, etc
a. Sample from the previous measurement may have been left on the prism surface.
b. Distilled water was left on the prism surface.
c. Measurement was executed without closing the cover lid.
2) If the operator of the RX-7000α makes any mistakes in operation or inputting of
work, the RX-7000α displays the corresponding error message.
a. Zero adjustment was performed with a sample.
b. Measurement was started with nothing on the prism, or zero adjustment was
started nothing on the prism .
In case of the above malfunctions, the end user can resolve by ensuring the above points are
checked and avoided. Carry out operation and measurement again. Perform step 3
(Periodical inspection of the preceding clause).
3) Failure in machinery or parts
When the RX-7000α does not operate properly due to mechanical trouble,
under the same measuring conditions, degradation of the parts inside the unit and/or
failure is assumed. The RX-7000α may be in need of repair.
a. Consult with the supervisor of the refractometer.
b. Record the trouble on an operating work report.
c. Contact the company where the refractometer was purchased.
d. Contact the manufacturer of the refractometer. (Make a contact list of the
company where the refractometer was purchased along with the manufacturer.)
e. In the case of repair, it becomes impossible to use the refractometer. Thus,
decide on methods of care and put them in writing.

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Standard Operating
Procedure （SOP）
Automatic digital
Refractometer
RX-7000α(alpha)
7. Establishment and record of revision
When this "standard operation procedure document" is revised, record the revised or changed
contents in this sheet and sign approval column.
Record of revision
Approval column
Author's
signature
Approved
by:
Date Establishment