ATP Electronics LBY-420 User manual

User Manual
1. Principle of Operation
2. Preparation for Use
3. Basic Operation
4.Clock Setting
5. Result Thresholds
6. Sample Measurements and Test Results
7. Transferring the Test Results to PC
8. Instrument Beeps
9. Faults
10. Technical Parameters

ATP bioluminescence detecting system
1. Principle of Operation
ATP bioluminescence detector converts invisible ATP
concentration to visible light output by bioluminescent chemistry
technology, to display a quantitative result of microbial population
indirectly. The result is a number from 0 to 9999 in Relative Light
Units(RLUs). RLUs are not a kind of tangible unit of light
measurement, but they can provide a real method to the ATP
bioluminescent test. In this application, 1 RLU is roughly equivalent
to 1fmol of ATP.
RLU value is compared with the result threshold set by user to
offer an overall result, which is pass , caution or failed
In order to ensure that you can get timely and accurate testing
result by ATP bioluminescence detecting system, please note the
following:
Before you begin, please read this user's manual carefully.
ATP bioluminescence detector should be auxiliarily used with
the ATP sampling swab.

2. Preparation for Use
Before you begin, please ensure you have read this user's manual
carefully and understood the system composition and method of
application of this instrument. Please confirm the integrity of the
product you bought according to the elements notedin the user's
manual.
mainfram
front and back
AA batteries or
rechargeable
batteries
Data line

Elements of ATP bioluminescence detecting system
Liquid Crystal Display：display test procedure and result
Unit lid：kill the light
Lable：display the instrument type and serial number
Battery compartment：fit two AA batteries
Up button：check last test result, and menu selection
Down button：check next test result, and menu selection
Start button：start test
Threshold set button：set threshold
Time set button：set time
Power on/off button：Power on/off the instrument
Special function button：Special function, such as enter delete
interface

Opening the lid reveals the following internal features:
Swab pocket: insert ATP sampling swab
Data interface: transfer the data to PC by data cable.

Instrument
indication

Saving introduction:
The testing code is a three-digit number from 001 to 999. The
number “1” after testing code refers to group one, and “2” refers to
group two. For example, “048 2” can be regarded as the 1048th
sample.
Fitting the batteries
The instrument is designed to operat from 2 AA batteries. The
batteries are fitted by openning the battery compartment cover on
the back of the instrument, and inserting two batteries with the
positive end towards top of the
instrument. After fitting the batteries,
the instrument powers on automaticly,
and enters into clock setting mode.
Date and time setting is discribed in
Section 4.
Caution: be careful not to swap the batteries’ poles over,

for this may destroy the electronic system of instrument
Tip: for better results, please use the batteries with good
quality and replace them as soon as they become low.
3. Basic operation
3.1 Power on the instrument
Press the button, and the instrument will beep once and
display the startup picture. Afterwards, the instrument will perform
an internal self-calibration check programme for 30 seconds.
Tip: If the clock is not set, the instrument will enter the time
and date set-up mode firstly (see section 4) and then perform
its self-calibration when the clock is set.
3.2 Internal self-calibration
When the instrument is turned on (see section 3.1), it performs
an internal self-calibration check, with the display counting down
from 30 to 0 seconds:

Tip:During self-calibration, there must be no ATP Swab in
the instrument and the lid must remain closed. If the icon
is flashing, close the lid.
The instrument will automatically perform the
self-calibration routine (the flashing CAL icon) under the
following circumstances;
a) When the instrument is in continuous operation for a
prolonged period of time (typically >30 minutes), and
b) The instrument is used in an environment where the
temperature changes significantly (typically >5℃).
If the E1 error code was showed in self-calibration, it
means the the temperature is higher than operating
temperature range, and it may caused by the reasons as
followed:

a) the operating temperature is higher than normal range,
and
b) The instrument is stored at the temperature exceeding
normal range:to make the instrument adopt to normal
temperature range, and
c) the instrument is damaged or in fault.
3.3 Ready for use：
Once the instrument has successfully performed its
self-calibration, it is ready to perform a measurement:
The detection procedure is discribed in Section 6.1
3.4 Power off the instrument
Press the button. The instrument will beep once and the
display will go blank.
TIP: the button is disabled while the instrument is

performing a mesurement.
3.5 Power Saving Standby Mode
If the unit is turned on and has stood by for more than 10
min(on operation for more than 10 min), it will automatically enter a
power saving standby mode.
To turn the unit back on, please press the button
3.6 Low Battery Indicator
The icon indicates the state of the batteries
-------------------------------------------------------------------------------
Not visible High
Visible Low, replace soon
Flashing Flat, replace now
When the batteries are flat, the unit will flash the icon,beep
three times, and then automatically turn off.
TIP:if the batteries are too flat, the unit will not power on. Store the
instrument in a cool dry place when not in use, as elevated
temperatures will shorten the battery life.

4. Clock Setting
With the unit turned on and ready for a measurement, press
button to display the current date and time.
To change the time and date, press the button. Then use the
and buttons to change the flashing value, and the button to
accept each new value in turn.
TIP: press button at any point will cancel this set-up
mode, leaving the time and date unchanged.
When batteries are fitted firstly or replaced, the
instrument powers on automaticly, and enters into clock
set-up mode. Once the clock is set, the unit will continue with
its internal self-calibration.
If the time is set incorrectly, the instrument will beep
once and display wrong time after the button is pressed,
and return to clock set-up mode.

5. Result Thresholds
The instrument can store up to 100 thresholds (0 to 99),each of
which defines a pair of upper and lower thresholds for the
measurement result.
Note: the different thresholds indicate specific sanitary
requirement of different testing sample. For the details how to
choose the thresholds, please contact your local hygiene and
epidemic prevention department, or set your own thresholds.
5.1 Changing the Thresholds Number
With the instrument turned on and ready for a measurement,
the thresholds number can be changed by pressing the button,
and use the and buttons to change the flashing
thresholds number, then press the button to accept the new
value.

TIP:Pressing the button again will exit this set-up mode,
leaving the thresholds number unchanged.
5.2 Changing the Thresholds Range
Press the button, use the and buttons to select the
required thresholds number , and then press the button.
Afterwards, use the and buttons to first change the value of
the upper threshold followed by the button; and then to change
the value of the lower threshold followed by the button to store
the new values:
TIP: Pressing the button at any point will exit this set-up mode,
leaving both the thresholds number and thresholds value
unchanged.
6. Sample Measurements and Test Results
6.1 Sample Measurement
With the instrument turned on and internal calibration done,
thepreparation of the new measurement is compeled.

the thresholds number, the value of upper and lower
threshold and test data number(68 indicates there are 68 test
results saving in the instrument)
TIP: When the stored test results take up more than 95% of
the memory, the MEM icon will flash. When the memory is
completely full, no more tests can be performed until the
memory has been deleted or uploaded to the PC(see to
section 6.3 and 7)
To perform a sample measurement, follow the steps below:
1.Activate the ATP swab
2.Ensure that the outside of the swab is clean and dry
3.Open the lid, insert the swab into the instrument, and
close the lid.

4.Press the button and wait 15 seconds for the result to
be displayed
While the measurement is being performed, the display
shows the new test number while the timer counts down from
15 to 0 seconds:
TIP: For consistent results, always keep the unit upright
and steady while it is performing a measurement to ensure
that the liquid in the ATP swab device is at the bottom of the
tube.
When the measurement is complete, the new test reading and
pass/caution/fail result (see section 5) are displayed. When the
icon flashes, take out the swab, and press the button, and the
instrument will get ready to the next measurement.

The indication of icons:
――――――――――――――――――――――――――
Test result≤ lower threshold
Test result＞lower threshold≤ upper threshold
Test result＞upper threshold .
――――――――――――――――――――――――――
TIP: After measurement, make sure to remove the swab to
prevent the liquid leakage of swab and damage the instrument
6.2 Viewing Stored Test Results
With the unit ready to perform a reading, the previous stored
test results can be viewed by pressing the and buttons:

The display then shows the latest test result, with the MEM
icon lit and the test number flashing. Press the button to scroll
backwards through the stored test results and the button to
scroll forwards.
Press the button to view the time and date that the test
was performed.
To exit the results review mode, please press the
button.

6.3 Deleting the Results Memory
The results memory can be completely deleted by entering the
results review mode (see section 6.2 above) and then pressing and
holding down the button for more than 2 seconds.
The display then shows the total number of results that will be
deleted, with the DELETE? Icon flashing.
The delete function is completed by pressing and holding down
the button for more than 2 seconds, or can cancelled by
pressing any other button.
7. Transferring the Test Results to PC
CD-ROM data processing software which is randomly equipped
can be used to upload the stored results to PC.
7.1 Install transmission procedure software
Insert CD into PC, then install according to installation tips.