Avantik QS11 User manual

Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
1
Avantik
Cryostat
INSTRUCTION MANUAL

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
2
CERTIFICATION
Avantik certifies that this instrument has been tested and checked carefully.
Its technical data was verified before shipment to be in accordance with the
published specifications.
The instrument complies with applicable international safety regulations.
WARRANTY
This Avantik product is warranted against defects in material and workmanship
for a period of 1 year. Parts which prove to be defective during the warranty
period will be repaired or replaced free of charge by the manufacturer. No other
other warranty is expressed or implied. Unauthorized modification or repair by third
party persons will void the warranty.
The warranty will expire in case of improper or wrong use of the instrument and in
case the warning and precautionary messages are not observed. Avantik is not
liable for any occurring damage.
Errors and omissions excepted. Subject to amendment and improvement without
further notice.
This instruction manual will be supplied together with each instrument. Further
copies can be ordered at the nearest Avantik sales office by giving the serial
number of the instrument, the number of the instruction manual and the date of
issue.
This instruction manual is available in the following languages:
Cat. No.
English: 387779

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
3
INTENDED USE
Dear valued Customer,
Thank you for buying this Avantik instrument.
Before putting the instrument into operation, please read these operating
instructions carefully to familiarize you with its proper operation and
functions.
The Cryostat QS11 / QS11UV is a highly efficient cryostat for sectioning
techniques in routine and research.
Only skilled or specially trained personnel must operate the microtome
cryostat, i.e. placing the specimen onto a chuck, sectioning and transferring
sections onto a slide. The listed and marked safety measures as well as the
regulations and hygiene measures of your respective lab must strictly be
observed.
Serial No.: __________________
Please check the Serial No. on the type plate, which is placed on the rear
side of your instrument and enter this number here. This way, questions and
service can be handled faster.
Instruction Manual No. 387779
Issued on January 27th, 2011
Avantik
36 Commerce Street
07081 NJ Springfield
USA
Phone: [phone]
Fax: [phone]

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
4
Intended Use
Table of Contents
Safety Precautions
PART 1 INTRODUCTION
1-1 Description of the QS11 / QS11UV
1-2 Technical data QS11 / QS11UV
PART 2 OPERATING INSTRUCTIONS
2-1 Setting up the cryostat
2-2 Initial turn-on
2-3 Basic operational rules
2-3-1 Actual and set value of the chamber temperature
2-3-2 Defrosting the evaporator
2-3-3 Interrupting a defrosting cycle
2-3-4 Emptying the defrosting liquid
2-3-5 Illumination of the cryo chamber
2-3-6 Freezing on a specimen
2-3-7 Heat extractors (optional accessories)
2-3-8 Specimen orientation with zero positioning
2-3-9 Handwheel lock
2-4 Cutting process
2-4-1 Cutting movement and retraction
2-4-2 Setting section thickness and trimming thickness
2-5 Feed
2-5-1 Specimen coarse feed
2-5-2 Memory function
2-5-3 Trimming and first cuts
2-5-4 Fine feed
2-6 Indication of cutting processes
2-6-1 Section counter
2-6-2 Sum of section thicknesses
2-6-3 Remaining travel to front end position
2-6-4 Setting the real time
2-7 Knife carriers
2-7-1 Standard knife carrier
2-7-2 Disposable blade carrier
2-7-3 Disposable blade carrier EC 70
2-8 Module U (Optional)

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
5
2-9 Error code indication
2-9-1 Definition of the error codes
2-10 Accessories
2-10-1 Standard equipment
2-10-2 Additional equipment
PART 3 THEORY OF OPERATION
3-1 Microtome cooling, defrost cycle
3-2 Cutting movement
3-3 Specimen coarse feed and trimming stages
PART 4 WORKING WITH THE CRYOSTAT
4-1 Preparations on the microtome and inside the cooling chamber
4-2 Freezing on the specimen
4-3 Orientation and trimming of specimens
4-4 Temperature list for cryo sectioning
4-5 Sectioning and taking off sections
4-6 How to avoid malfunctions
4-6-1 Possible sources of errors – cause and removal
PART 5 MAINTENANCE AND CARE OF THE CRYOSTAT
5-1 Shutting-off for cleaning
5-2 Removing the microtome
5-3 Cleaning and care of the microtome
5-4 Cleaning the cooling lamella
PART 6 CONDITIONS FOR THE TRANSPORTATION OF THE INSTRUMENT
6-1 Taking back the instrument for repair or routine maintenance
6-2 Disposal of the instrument after final shutdown

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
6
SAFETY PRECAUTIONS
WARNING SIGNALS AND SYMBOLS
The installation and routine use of the QS11 / QS11UV is easy and safe if the instructions in this manual are
being observed.
Note:
Special instructions regarding operation of the instrument.
Warning:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.
Caution – general danger spot:
The instruction manual must strictly be observed whenever this symbol is visible on the
instrument.
Biohazard:
Warning of biological danger.
Radioactivity:
Warning of radioactive danger.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European countries with a
separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
7
SAFETY PRECAUTIONS
C A U T I O N !
The operator's safety is affected, when the instrument is not operated in accordance
with this instruction manual.
Please observe the following general precautions during operation of this
instrument. Failure to comply with these precautions violates safety standards and
the intended use of the instrument. Avantik is not liable for misuse of the
instruments and failure to comply with basic safety requirements.
INSTRUMENT GROUNDING
To avoid injury from electrical current, the instrument must be connected with the protective earth. The
instrument is equipped with a three wire ground plug. The power outlet must be connected to the protective
earth and must meet the International Electrotechnical Commission (IEC) regulations.
CAUTION: MAINS VOLTAGE
Never remove instrument covers during operation. Component replacements as well as adjustments must
only be made by trained service personnel. Unplug the unit before removing or opening the covers.
DANGER IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable gases.
HAZARD OF FROSTBITE
Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at
unprotected hands and arms.
HAZARD OF RADIOACTIVE RADIATION
When working with radioactive specimens observe all applicable radiation safety procedures. When working
with radioactive contaminated material, appropriate safety and disinfection measures must be carried out.
According to the rules and regulations concerning the handling of radioactive contaminated material of the
respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
8
HAZARD OF INFECTION
Use the appropriate safety and disinfection measures when working with infectious specimens.
According to the rules and regulations concerning the handling of infectious/radioactive contaminated
material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must
be worn.
HAZARD OF BIOLOGICAL DANGER
Specimens used during the intended operation of the instrument might potentially be infectious. For this
reason, it is recommended to observe the general laboratory regulations concerning protection against
danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be read in
the Laboratory Biosafety Manual : 1984 of the World Health Organization.
HAZARD OF MALFUNCTION
To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic
environment. This means, that transmitters such as mobile phones must not be operated in their close
vicinity.
In case of malfunctions and/or service work, please turn off the instrument and contact your local dealer.
CARE IN USING MICROTOME KNIFE
To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting specimen
and knife. If possible, the specimen should be clamped in before the knife is inserted into the knife holder.
Before changing the knife holder, always remove blade or knife! Unused knives should always be kept in a
knife case. Never place the knife with the cutting edge upwards. Never try to catch a dropping knife!! Never
check the sharpness of the cutting edge with your fingers. The cutting edge is extremely sharp!
WASTE DISPOSAL
All debris, waste, defrosting liquid as well as infectious and radioactive contaminated material from operation
must be disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning
liquids as well as section waste must be disposed of according to the respective regulations for special
waste!

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
9
Fig. 1

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
10
1 INTRODUCTION
1-1 DESCRIPTION OF THE QS11 / QS11UV
Open top cryostat. Stainless steel cooling
chamber. Chamber temperature regulation
variable from +5°C down to –35°C.
Graphic LED-display of set and actual
temperatures, electronic control with user-oriented
touchpad keyboard, battery-buffered memory and
self-diagnostic system.
Automatic defrosting and in addition a manual
defrost cycle which can be activated when
needed. Defrost interrupt on keystroke request.
Integrated fast freezing device down to max.
–60°C via Peltier element.
Sliding window with heater. Integrated fluorescent
lamp for cryo-chamber. Brush shelf and storage
space inside the cryo chamber. Large, flat storage
space on top of the housing with integrated
storage space for marked slides.
Rotary Microtome with backlash and
maintenance-free cross roller bearings in stainless
steel. Electro-mechanical feed system.
Section thickness setting from 1 to 20 microns;
up to 10 µm in 1 µm-increments,
up to 20 µm in 2 µm-increments,
Trimming thickness setting from 10 to 500
microns;
up to 80 µm in 10 µm-increments,
up to 100 µm in 20 µm-increments,
up to 300 µm in 50 µm increments and
up to 500 µm in 100 µm-increments.
Specimen retraction during the return stroke with
optical indication. Horizontal feed range 28 mm.
Vertical cutting stroke 60 mm.
Max. specimen size 70 x 55 mm.
Motorized coarse feed in two directions.
Limit indication and automated switch off at front
and rear limits of horizontal travel.
Section counter and indication of sum of section
thicknesses with reset-button. Indication of
remaining travel.
Mechanical handwheel brake in the lower reversal
point of the specimen orientation.
Fine orientation of specimen 8° universally in x/y-
axis with zero device. Rotatable on Z-axis, 360°.
Basic outfit with 3 x 30 mm chucks, 1 x 40 mm
chuck, 118 ml freezing medium, 100 ml cryostat
oil, brush shelf and section waste tray, consisting
of two parts.

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
11
VU11SQ/11SQATADLACINHCET2-1
Chamber temperature control:.............................................................................+5°C to -35°C
Fast freezing station via Peltier element
depending on the chamber temperature: ...................................................down to max. -60°C
Defrosting: automatic defrosting, adjustable..............with temperature control
gnitsorfedetaidemmilaunam
Microtome: Section thickness range:.............................................. 1...500 µm
htnoitceseniF icknessrange:..........................................1...20 µm
:noituloseR ...................................................... 1 µm for 1...10 µm
mµ2........................................................................ for 10...20 µm
senkcihtgnimmirT s range:......................................... 10...500 µm
:noituloseR ....................................................10 µm for 10...80 µm
mµ02.................................................................... for 80...100 µm
ofmµ05.................................................................. r 100...300 µm
ofmµ001................................................................ r 300...500 µm
udnoitcarternemicepS ring return travel..............................40 µm
micepslacitreV en stroke ................................................... 60 mm
micepslatnoziroH en movement..........................................28 mm
Read-outs: Five-digit LED-display: section thicknesses, section counter, sum of
iniamer,sessenkcihtnoitces ng travel to front end position and
erutarepmetrebmahc
Coarse feed: ............................... motorized, graduated, single and continuous
Size of chucks: .......................................................................................30, 40 mm
laiceps............................................................. sizes upon request
Specimen orientation: x - and y - axes: ....................... universal 8° with zero positioning
:sixa-z ........................................................................ up to 360°
Cooling chamber illumination
Sliding window
Cutting drive: ................................................................ manuallyvia handwheel
Cutting area: ......................................... horizontal specimen movement 28 mm
mm06ekortsnemicepslacitrev.....................................................
All temperatures refer to an ambient temperature of +20°C! Cont'd on page -2-

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
12
page -2-
VU11SQ/11SQATADLACINHCET2-1
Handwheel brake: ..................................................................................... mechanical
Transportation and storage conditions
Storage temperature range: ........................................................................ -20°C up to +50°C
Operating conditions: ........................+5°C up to +35°C (at a max. rel. humidity of 60%)
itla.......................................................... tude up to 2000 m M.S.L.
irof.......................................................................... ndoor use only
roolF.......................................... loading requirements: 230 kg/m2
Power requirements: ............................................................ 100V/50 Hz, 12 A, +/-10%
zH06/V001............................................................. , 12 A, +/-10%
zH06/V511............................................................. , 12 A, +/-10%
05/V032...022...................................................... Hz, 6 A, +/-10%
zH06/V022............................................................... , 6 A, +/-10%
zH05/V042............................................................... , 6 A, +/-10%
Internal protection
Primary circuits:
Transformer: .......................................................................T5.0AH, 100...115 V
5.2T....................................................................... AH, 220...240 V
Illumination: .......................................................................T0,8AH, 100...115 V
4,0T....................................................................... AH, 220...240 V
Secondary circuits:
Window heating: ........................................................................................... T2,5AH
CPU: ..............................................................................11 VAC/T0.5AH
Panel: ..............................................................................11 VAC/T0.5AH
Stepping motor/valve: ..............................................................................24 VAC/T2.5AH
Fast freezing station: ..........................................................................2.3/8 VAC/T8.0AH
Pollution degree: ......................................................................................................2
Overvoltage category: ......................................................................................................II
Sound pressure: ......................................................................................... 45 dB(A)
............................... measured with 1 m distance to the instrument
Fluids and gases: .................................................................... cooling agent: R404a,
illif................................................... ng amount: 100...120 V: 250 g
...022.......................................................................... 240 V: 230 g
Dimensions: ..Wide (w/o handwheel): 640 mm, deep: 760 mm, high: 1200 mm
Weight: ...................................................143 kg (depending on the model)
All temperatures refer to an ambient temperature of +20°C!

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
13
Fig. 2
1 = LED retraction
2=Section thickness setting
3 = Trimming thickness setting
4 = LED fine feed
5 = LED trim feed
6 = Coarse feed, backwards
7 = Coarse feed, forwards
8 = Trim button
9 = Scroll button
10 = Display, left
11 = Reset button
12 = Memory button
13 = Setting defrost time
14 = Interruption defrosting cycle
15 = Down button and activation of Peltier element
16 = Up button
17 = Chamber illumination
18 = Display, right
MEMO
COUNT
SUM
TRAVEL
1
2
3
45678910
SECTIONTHICKNESS µm
RETRACT
TRIM THICKNESS µm
CHAMBERTEMPERATURE ° C
DOWN
DEFR
TIME INTER
RUPT
UP
12 14
16
18
20
10
20
3040 50 60 70 80 100 150 200
300
400
500
250
MICROM
HM525
P
1
2
3
4
5
67
8
9
10
11
12 13
14
15
16
17 18

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
14
2 OPERATING INSTRUCTIONS
2-1 SETTING UP THE CRYOSTAT
Unpacking the instrument:
•Cut through the three packing straps and
remove the packing.
•Remove the upper wooden cover (fig. 3.1).
•The hinges of the upper wooden cover are
secured with tape.
•Remove this tape.
•Lift the packing (fig. 3.2) over the instrument.
•Remove the upper and lower supporting
foams (fig. 3.3, 3.4, 3.5, 3.6) from the
cryostat.
Note:
The upper wooden cover now serves
as an incline (fig. 3a.1) on which the
cryostat can be moved from the palett
to the floor by using the handle (fig. 3a.2)
•The hinges of the upper wooden cover (fig.
3.1) are inserted into the screws of the
bottom plate (fig. 3a.3). The correct side for
this is marked with arrows.
• The tool (fig. 3b.2, size 30) for the height
adjustment of the setting feet of the cryostat
is packed together with the accessories of
the cryostat (separate carton on the foot rest
of the cryostat).
•Turn the setting feet completely upwards via
this tool so that the cryostat stands with its
rollers on the bottom plate (fig. 3a.3).
•Via the handle (fig. 3a.2) roll the cryostat
from the bottom plate (fig. 3a.3) over the
wooden cover (fig. 3a.1). In doing so, the
rear side of the instrument must show to the
incline (fig. 3a.1).
Caution:
The instrument is very heavy. While
the instrument rolls down on the
incline, it must be guaranteed that the
instrument does not move in an uncontrolled
way.
•Now the cryostat can be rolled to its site of
installation.
•For possible return shipments, please keep
the packing.
Fig. 3
Fig. 3a
Fig. 3b
HM550MVD
3
0
1
2
1
2
3
6
5
4
7
1
3
HM520
2
1
2

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
15
Warning:
The instrument must only be moved in
standing or slightly tilted (approx. 30°)
position.
Choose installation site that
•enough ventilation for the cooling system is
guaranteed.
•the distance between wall and rear panel is
approx. 15 cm. For this, install the distance
bolts (fig. 4a.1).
•the suction areas (fig. 4a.2) on either side are
kept free.
•the mains switch for separating the
instrument from the power supply is
accessible any time.
Moreover, the installation site must be free
from:
•draught by open doors or by air conditioning
systems.
•direct exposure to sunlight into the cooling
chamber.
Note:
Both measures reduce the formation of
frost and therefore result in more
favourable work conditions. A high air
moisture as well as high ambient temperatures
reduce the maximum performance of the
instrument.
•To fix the complete unit, tighten the screws
(fig. 4.1) by using the attached tool.
•Install the separate packed handwheel
handle.
Warning:
Instead of the handwheel handle, a
transportation screw (fig. 4b.1) is
inserted into the handwheel. This way,
the handwheel is tightly and securely
connected with the housing of the cryostat. It
is absolutely necessary to remove this
transportation screw before the initial turn-
on!
•Loosen the transportation screw (fig. 4b.1)
via the Allan key, size 6.
•Now fix the separately packed handwheel
handle on the handwheel with the attached
screw by means of the Allan key.
Fig. 4
Fig. 4a
Fig. 4b
SW13
1
1
2
1

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
16
2-2 INITIAL TURN-ON
Note:
The kind of the used examination
materials and all special conditions for
their processing, pre-treatment and, if
necessary, storage as well as instrument
controls for correct and safe operation is in the
responsibility of the operator.
The operator is also responsible for special
equipment and materials and/or reagents for
the operation of the instrument.
Warning:
Before turning on the instrument for
the first time, please check if the power
requirements indicated on the type
plate (fig. 6) correspond to the power supply
voltage being used.
•No other instruments should be connected to
the circuit used for the cryostat, as the
compressor needs high surge currents when
started.
•Do not use multi-socket power outlets with
small wire sizes for the supply of the
instrument.
•We recommend to immediately install all
accessories belonging into the cryo chamber,
e.g. blade carrier.
•The instruction manual of the instrument can
be found in the separate carton which is
placed on the foot part of the cryostat.
•Connect the power line of the instrument to
the power outlet.
•Turn on the power switch (fig. 5.1) on the
rear side of the instrument.
•Close the heated sliding window and cool
down the instrument.
The cooling phase will take several hours
depending on the set chamber temperature.
Fig. 5
1 = Power switch
2= Mains plug
Fig. 6
I
0
1
2
Avantik

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
17
2-3 BASIC OPERATIONAL RULES
After having turned on the instrument, the two
displays show the set chamber temperature as
well as the number of sections, the section
thickness sum as well as the remaining travel
to the front end position. Section thicknesses
are indicated via LEDs.
2-3-1 ACTUAL AND SET VALUE OF
THE TEMPERATURE
The cooling of the microtome chamber can be
controlled by the corresponding part of the
operating controls. The actual temperature of
the microtome chamber is shown in °C on the
digital display (fig. 7.1). Briefly press the UP- or
DOWN-button (fig. 7.2 or 7.3), the set value of
the temperature in the microtome chamber is
shown for approx. 2 sec. Afterwards, the
display shows again the actual value of the
temperature of the microtome chamber. To
change the set value, press permanently the
UP-button (fig. 7.2) or DOWN-button (fig. 7.3).
The UP-button leads to higher, the DOWN-
button to lower temperatures.
The valid range of the set value goes from
+5°C down to -35°C. If the UP-button is used
beyond the limit of +5°C, the display shows
" - - -" and the temperature control is turned
off. Using the DOWN-button the cooling
system is turned on again.
Fig. 7
MEMO
CHAMBERTEMPERATURE ° C
DOWN
DEFR
TIME INTER
RUPT
UP
P
1
32

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
18
2-3-2 DEFROSTING THE
EVAPORATOR
Every 24 hours the evaporator in the rear part
of the microtome chamber is defrosted
automatically.
During a defrosting process, the display shows
a temperature with the letter H. This
temperature refers to the heating of the
evaporator and not to the temperature of the
cooling chamber, which is normally shown.
This value, however, is of no importance for
the operation of the instrument.
Press the button DEFR TIME (fig. 8.4) and the
set defrosting time is shown for approx.
2 sec. on the display (fig. 8.1). It is advisable to
set the time of the defrosting process not
during routine working time. To change the
starting time of defrosting at intervals of
15 minutes, simultaneously press the button
DEFR TIME (fig. 8.4) and the button UP (fig.
8.2) or DOWN (fig. 8.3). Defrosting can only be
carried out at the set defrosting time, if the real
time is set correctly (see 2-6-4).
After finishing the adjusting process, the
display shows the set temperature of the
microtome chamber after two more seconds.
Note:
An immediate defrosting cycle can be
released at any time. For this, press
the button DEFR TIME (fig. 8.4) and
additionally the reset button (fig. 2.11).
2-3-3 INTERRUPTING A DEFROSTING
CYCLE
If needed, the daily defrosting can be
interrupted or cancelled. Press the button
INTERRUPT (fig. 8.5), the LED in this button
lights up. If the function INTERRUPT is chosen
during the defrosting period, the LED blinks for
the rest of the suppressed defrosting process.
A cancelled or interrupted defrosting process
must be repeated later on, as otherwise the
evaporator will cover completely with frost and
cannot cool any more.
For this, press the button (fig. 8.5) again to turn
off the function INTERRUPT (LED is off). The
next defrosting process will then be started
when the next pre-selected defrosting time is
reached.
Fig. 8
MEMO
CHAMBERTEMPERATURE ° C
DOWN
DEFR
TIME INTER
RUPT
UP
P
1
32
4 5

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
19
2-3-4 EMPTYING THE DEFROSTING
LIQUID
Note:
The liquid from the daily defrosting
process is collected in the container
(fig. 9.1). Please note that this
container is emptied in due time according to
the safety precautions in this instruction
manual.
We highly recommend to disinfect the
container with a surface disinfection solution
after having emptied it.
2-3-5 ILLUMINATION OF THE COOLING
CHAMBER
To illuminate the microtome chamber, a
fluorescent lamp is above the chamber, which
can be turned on and off by means of the
button (fig. 10.1).
At low temperatures and after long periods of
being turned off, the lamp might flare when
turning it on.
Fig. 9
Fig. 10
MEMO
CHAMBER TEMPERATURE ° C
DOWN
DEFR
TIME INTER
RUPT
UP
1
12

Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
20
2-3-6 FREEZING-ON OF SPECIMENS
There are several possibilities to freeze-on
specimens.
Various specimen chucks are available. Round
specimen chucks can be supplied with a
diameter of 30 mm and 40 mm and rectangular
specimen chucks with a size of 50, 55, 60 and
70 mm. Special sizes on request.
For the especially fast and almost artefact-free
freezing-on, the QS11 / QS11UV is equipped
with a Peltier element (fig. 12.1) (down to max.
–60°C surface temperature).
•Briefly press the down button (fig. 10.2)
twice to activate the Peltier element.
•Put the warm chuck into the hole (fig. 11.1)
of the fast freezing rail.
•Put freezing medium onto the chuck.
•Place fresh tissue onto the still liquid
freezing medium.
•When the medium and tissue are frozen,
the chuck can be inserted into the
specimen clamping and can be fixed.
•The specimen can now be sectioned.
•The remaining 23 stations of the fast
freezing station can also be used for
freezing-on or storing specimens.
Note:
The upper side of the freezing station
must not be covered by frost or ice. To
avoid this, use ethanol or the like.
Note:
The Peltier element automatically turns
off after 8 min.
Caution:
With regard to hazard of frostbite, it is
absolutely necessary to observe the
safety precautions listed at the
beginning of this manual.
Fig. 11
Fig. 12
Fig. 13
1
2
3
10
11
19
20
21
22
23
24
13
8
9
26
27
17
18
25
5
6
7
12
4
14
15
16
1
1