Baker Bugbox Plus UM-017 User manual
Bugbox Plus
UM-017
Bugbox Plus User Manual
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Bugbox Plus
UM-17 R4.0 Page 2of 47 Bugbox Plus User Manual
Product Summary
Baker Ruskinn’s anaerobic workstations are designed specifically to help microbiologists cope with
rising workloads and provide the best primary isolation rates. The world's smallest microaerophilic
workstation packs all of the innovative features into a compact bench print.
Features and Benefits
Quick and easy direct access with the gloveless, cuffed Ezee Sleeve system.
Single plate entry system (SPES) –this optional mailbox-like slot allows quick side entry or exit
of individual plates, bypassing the interlock cycling process.
Energy saving lighting. Read plates easily under perfect illumination without O2 exposure.
High- intensity inspection spot lamp for close sample analysis is foot-operated for ease of
use.
Up to 180 90mm plate capacity.
Optimum cell environment.
oAccurate temperature control - from ambient + 5˚C to 45˚C.
oAccurate and automated humidity control - no dry spots.
oPalladium catalyst maintains anaerobic environment - plus anaerobic colour-
indicator strips verify anaerobic conditions.
oEzee Sleeve system allows access without disrupting atmosphere within the
chamber.
Economic and reliable long-term savings
oLow gas consumption and running costs.
oMinimal maintenance and downtime - with annual or biennial preventative
maintenance kits available.
*Note. The use of the word Interlock in this manual is medical terminology, and refers to the
environmental working area between both chambers. It does not refer to the engineering safety
interlock switch mechanism.
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Table of Contents
Product Summary ...................................................................................................................................2
Features and Benefits ............................................................................................................................. 2
Overview ....................................................................................................................................6
Safety Instructions ........................................................................................................ 6
Regulatory compliance ................................................................................................. 7
Symbols......................................................................................................................... 7
Installation and relocation............................................................................................ 8
Weight and dimensions ................................................................................................9
Gas and electrical supply requirement....................................................................................10
Gas supply requirements ............................................................................................10
Gas regulator requirements........................................................................................10
Electrical supply requirements ...................................................................................10
Voltage and frequency requirements.........................................................................10
Power consumption....................................................................................................10
Powering the workstation .......................................................................................... 11
Workstation overview..............................................................................................................12
Workstation layout .....................................................................................................12
Component layout ......................................................................................................12
Front view .......................................................................................................12
Rear view ........................................................................................................13
Left side view –Standard humidifier.............................................................. 14
Left side view –Ultrasonic humidifier ............................................................15
Right side view................................................................................................16
Control system layout.................................................................................................17
Control panel ..................................................................................................17
Temperature controller ..................................................................................18
Port selector knob controller.......................................................................... 18
Foot switch control panel ...............................................................................19
Power entry panel .......................................................................................... 19
Interlock inner door control panel..................................................................20
Temperature and humidity control .........................................................................................21
Temperature control...................................................................................................21
Setting the temperature;................................................................................ 21
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Humidity control .........................................................................................................21
Standard humidity control overview ..............................................................21
Environmental gas composition control..................................................................................22
Environmental control overview ................................................................................22
Using the anaerobic indicator strips........................................................................... 22
Using the workstation..............................................................................................................23
Using the interlock......................................................................................................23
Interlock overview ..........................................................................................23
Opening the interlock outer door................................................................... 23
Opening the interlock inner door ................................................................... 23
Transferring material into the workstation chamber via the interlock..........24
Removing material from the workstation chamber via the interlock ............24
Hand access to the main chamber.............................................................................. 25
Ezee Sleeve overview...................................................................................... 26
Entering the workstation chamber using the Ezee Sleeves............................26
Exiting the workstation chamber using the Ezee Sleeves...............................27
Arm gas displacement volume and pressure changes ...................................27
Internal lights ..............................................................................................................28
Rear shelf ....................................................................................................................28
Optional extras............................................................................................................29
Single Plate Entry System (SPES) .................................................................... 29
Internal power socket.....................................................................................31
Cable gland .....................................................................................................31
Universal cable gland......................................................................................31
Gas sample port..............................................................................................32
Vacuum port ...................................................................................................32
Online video user guides.............................................................................................32
Cleaning and maintenance ......................................................................................................33
Cleaning the workstation............................................................................................33
Cleaning agents...............................................................................................33
Cleaning procedure –during and after each use............................................ 33
Cleaning procedure –deep clean ...................................................................34
Maintaining the workstation –End user maintenance ..............................................35
Filling the pressure relief/ humidifier tank..................................................... 35
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Replacing an Ezee Sleeve................................................................................36
Replacing the detox and catalyst sachets.......................................................37
Replacing the mains plug fuse –UK users only ..............................................38
Replacing the mains fuses .............................................................................. 39
Service requirements.................................................................................................. 40
Spare parts and accessories........................................................................................40
Overview.........................................................................................................40
Spare parts and cleaning agents..................................................................... 41
Accessories......................................................................................................41
Workstation malfunction............................................................................................42
Common problems and solutions............................................................................... 42
Workstation general problems....................................................................... 42
Interlock problems..........................................................................................43
Gas consumption/ environmental control problems ..................................... 44
Warranty information.............................................................................................................. 45
Disposal information................................................................................................................ 46
Contact details .........................................................................................................................47
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Overview
Please read this manual carefully to familiarise yourself with the operation and maintenance of your
Bugbox Plus workstation. Note: The workstation should be located in a well-ventilated area.
Safety Instructions
For your safety, the safety of others using the workstation and those around you;
The covers on both ends and the top of the workstation must not be removed by anyone
other than a qualified service engineer. There are no end user serviceable parts within these
covers.
The AC Mains outlet that the workstation is connected to, MUST not be obstructed by the
workstation or any other equipment, and MUST be accessible in case of emergency. In case
of emergency, disconnect the workstation from the AC Mains Outlet.
In case of damage to the workstation disconnect the workstation from the AC Mains outlet
and contact your Local Distributor for advice
The workstation and stand must be connected to a protective earth.
Only the power cord supplied with the workstation should be used to connect the
workstation to the mains supply.
If an uninterrupted power supply is used, the Bugbox Plus must be connected to the
uninterrupted power supply.
Only Ruskinn Technology Limited replacement parts should be used.
Gas regulators must be used for each gas supply. A 2 stage regulator should be used for a
bottled gas supply. The maximum supply pressure permissible is 4 bar gauge.
The maximum permissible concentration of Hydrogen in the anaerobic mixed gas is 5.5%
Hydrogen.
The mains supply voltage fluctuations must not exceed +/- 10% of the nominal mains
voltage.
Only the gasses specified in this manual may be used.
The maximum power rating of the internal socket must not be exceeded.
The humidifier screw cap must not be covered or blocked.
The humidifier/ pressure relief tank must not be overfilled.
The spot light should not be used continuously for more than 10 minutes. The spot light
should be allowed to cool for at least 10 minutes between uses.
The cooling fan covers and cooling vents must not be covered or blocked.
The workstation should not be lifted by the glove ports, the interlock or the Single Plate
Entry System.
The weight limit for the rear shelf is 5.5kg. The weight must be evenly distributed.
The use of Radioactive materials in the workstation is strictly prohibited.
FAILURE TO ADHERE TO THESE SAFETY INSTRUCTIONS COULD CAUSE SERIOUS INJURY AND WILL
INVALIDATE THE WORKSTATION WARRANTY. RUSKINN TECHNOLOGY LIMITED ACCEPTS NO
RESPONSIBILITY FOR ANY ACCIDENT, INJURY OR LOSS CAUSED BY UNSAFE OPERATION OF THE
WORKSTATION
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Regulatory compliance
This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage
Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive
204/108/EC.
Symbols
Before using the Bugbox Plus, please ensure that you are familiar with the symbols on the Bugbox
Plus. Figure 1 explains the symbols found on the Bugbox Plus;
Figure 1: Bugbox Plus symbols
Symbol
Meaning
Refer to user manual.
~
Alternating current
O
Off
I
On
Primary earth connection
This product complies with the essential EEA
requirements for Electrical Safety and
Electromagnetic compatibility as set out in
the EMC directive 2004/108/EC and the Low
Voltage Directive 2006/95/EC
Caution, do not remove covers. No end user
serviceable parts behind covers. Please refer
to this manual in all cases where this symbol
appears, in order to find out the nature of the
Potential Hazard and actions to be taken in
order to avoid the Hazard.
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Symbol
Meaning
Warning, this equipment contains high
voltage circuitry.
Bugbox Plus contains hazardous components
and must not be disposed of at a household
waste site. Instead it should be taken to the
appropriate collection point for the recycling
of electrical and electronic equipment.
Date of manufacture in format
YYYY MM
Installation and relocation
Bugbox Plus should not be installed or relocated by anyone other than a qualified engineer. To arrange
installation or relocation, please contact your local distributor.
Bugbox Plus
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Weight and dimensions
The Bugbox Plus workstation weighs approximately 55kg. Figure 2 lists the dimensions of the Bugbox
Plus workstation;
Figure 2: Bugbox Plus workstation dimensions
External width
830 mm
External height
650 mm
External depth
660 mm
Workstation chamber internal width
500 mm
Workstation chamber internal height
420 mm
Workstation chamber internal depth
460 mm
Interlock internal width
150 mm
Interlock internal height
190 mm
Interlock internal depth
230 mm
Interlock outer door clear opening width
155 mm
Interlock outer door clear opening height
200 mm
Interlock inner door clear opening width
185 mm
Interlock inner door clear opening height
180 mm
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Gas and electrical supply requirement
Gas supply requirements
The Bugbox Plus workstation requires 2 gas supplies;
Anaerobic mixed gas (up to 5.5% hydrogen, up to 10% carbon dioxide, remainder nitrogen)
Oxygen free nitrogen.
The maximum permissible concentration of hydrogen in the anaerobic mixed gas is 5.5%. The use
of any other gas(ses) will invalidate the warranty and may cause serious injury. The Gas supplies
must be securely fastened after installation.
Gas regulator requirements
Gas regulators must be used with all gas supplies to the workstation. A 2 stage regulator must be used
for each bottled gas supply. Regulators should be available from your local gas supplier. Ruskinn
Technology Limited does not supply gas regulators.
The gas regulator should provide a minimum supply pressure of 3 bar gauge (42psi). The maximum
supply pressure permissible is 4 bar gauge. A supply pressure greater than this will damage internal
components of the workstation and will invalidate the warranty.
Electrical supply requirements
The workstation must be connected to a mains power supply. A power cord is supplied to connect the
workstation to the mains supply. Only the power cord supplied should be used to connect the
workstation to the mains supply. It is advised that the workstation be located no greater than 1 metre
from the plug socket. The plug socket should not be obstructed by the Bugbox Plus workstation. The
workstation must be connected to a protective earth.
Voltage and frequency requirements
To ensure safe operation of the workstation, it must be connected to a supply of the correct voltage
and frequency as shown in the rating label (item 2 Figure 8). The mains supply voltage fluctuations
must not exceed +/- 10% of the nominal mains voltage.
Power consumption
Figure 3 shows the power consumption ratings;
Figure 3: Power consumption ratings
Supply voltage and frequency
Power consumption
240V AC, 50Hz
150 W
110V AC, 60Hz
200 W
Note: The power consumption is for normal operating conditions with no equipment connected to
the internal power supply. Power consumption will vary dependent upon the conditions inside the
workstation chamber and the conditions inside the room the workstation is located in.
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Powering the workstation
To switch the workstation on, press the power switch (item 2 in Figure 13).
To switch the workstation off, press the power switch (item 2 in Figure 13 ).
Note: A glove port should be left open when the workstation is switched off, as the pressure inside
the workstation will decrease as the workstation cools. The open glove port will allow the pressure
inside the workstation chamber to equalise with the external pressure.
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Workstation overview
Workstation layout
The Bugbox Plus workstation consists of 2 main areas; the workstation chamber and the interlock
chamber. The workstation chamber is the main working area of the workstation. Access to the
workstation chamber is provided by the glove ports and the interlock. The interlock consists of the
interlock chamber, with an inner and an outer door. The Bugbox Plus workstation is controlled via the
control panel.
Component layout
Please familiarise yourself with the layout of your Bugbox Plus workstation.
Front view
Figure 4 shows the front view of the Bugbox Plus workstation;
Figure 4: Bugbox Plus workstation front view
1. Glove ports (shown without Ezee Sleeves).
2. Port entry selector knob.
3. Interlock outer door.
4. Control panel.
2
3
4
1
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Left side view –Standard humidifier
Figure 6 shows the left side view of the Bugbox Plus workstation with the standard humidifier;
Figure 6: Bugbox Plus workstation left side view
1. Pressure relief tank fill level indicator.
2. Pressure relief tank.
3. Pressure relief tank overflow pipe.
4. Pressure relief tank cover (pressure relief tank bung).
5. Condenser fan.
6. Single Plate Entry System (optional extra).
Note: Please locate a suitable container below the tubing to collect any excess water from the
overflow tube. (A container is provided in the accessory kit).
1
2
3
4
5
6
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Left side view –Ultrasonic humidifier
Figure 7 shows the left side view of the Bugbox Plus workstation with the ultrasonic humidifier;
Figure 7: Bugbox Plus workstation left side view with ultrasonic humidifier
1. Humidifier screw cap.
2. Humidifier tank.
3. Exhaust valve outlet.
4. Vacuum line (optional extra).
5. Condenser fan. (Plastic shown, metal now standard).
6. Single Plate Entry System (SPES), (optional extra).
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Control system layout
Please familiarise yourself with the control system layout of your Bugbox Plus workstation.
Control panel
Figure 9 shows the control panel of the Bugbox Plus workstation;
Figure 9: Bugbox Plus workstation control panel
1. Power indicator. Illuminates to indicate the workstation is connected to the mains and is
switched on.
2. Chamber light switch. Press to switch the chamber light on/ off.
3. Gas Alarm switch. Press to switch the gas alarm on/ off.
4. Humidistat. Controls the level of humidity inside the workstation chamber.
5. Interlock purge button. Press to initiate the interlock purge cycle.
6. Lock active indicator LED. Flashes to indicate that the interlock purge cycle is in progress.
7. Gas demand indicator. Illuminates when there is a low pressure condition inside the
workstation chamber.
8. ANO2low indicator. Illuminates to indicate that the anoxic mixed gas supply has run out.
9. N2low indicator. Illuminates to indicate that the nitrogen supply has run out.
10. Temperature controller. See section 4.1 for more details.
1
2
3
4
10
9
8
7
6
5
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Temperature controller
Figure 10 shows the temperature controller;
Figure 10: Temperature controller
1. Not user accessible (For service personnel only)
2. Not user accessible (For service personnel only)
3. Number scrolling button. Use to move across digits. Use with buttons 4
& 5 to increase and decrease the temperature.
4. Decrease temperature (0.1°C increments) or humidity.
5. Increase temperature (0.1°C increments) or humidity.
For temperature control, the white value (PV- Process Value) is the actual temperature. The green
value (SV- Set Value) is the required value (the set point). All temperatures are in oC.
For ultrasonic humidity control, the white value is the actual relative humidity and the green value the
required relative humidity (the set point). All humidity’s are percentage relative humidity.
Port selector knob controller
Figure 11 shows the port selector knob controller for hand access to the chamber (see section 0);
Figure 11: Port selector knob controller
1
2
3
4
5
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Foot switch control panel
Figure 12 shows the foot switch controller which is used to operate the spot light, vacuum control and
interlock purge control;
Figure 12: Foot switch controller
1. VAC for glove port vacuum control
2. LOCK for interlock purge control
3. SPOT for spotlight operation
Power entry panel
Figure 13 shows the power entry panel on the side of the workstation;
Figure 13: Power entry panel
1. Mains fuse holder.
2. On/ off switch.
3. Mains lead connection.
4. Nitrogen Gas inlet
5. Primary earth stud.
6. Anaerobic Mixed Gas Inlet (Max 5.5% Hydrogen in gas mixture)
1
2
3
1
2
3
4
5
6
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Interlock inner door control panel
Figure 14 shows the interlock inner door control panel;
Figure 14: Interlock inner door control panel
1. Interlock inner door LED indicator. Illuminates when the interlock inner door is open.
2. Interlock inner door button. Press to open the interlock inner door (when available).
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