Baker Bugbox M UM-020 User manual

UM-020
Bugbox M User Manual
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Bugbox M
UM-20 R4.0 Page 2of 47 Bugbox M User Manual
Product Summary
The world's smallest microaerophilic workstation packs all of the innovative features into a compact
bench print. Incorporating all of the familiar and proven features of the Bugbox, but tailored to
interface with the ICO2N2IC Advanced Gas Mixing System.
Like the Bugbox, taking just 800mm of bench space with a capacity to incubate up to 200 plates, the
Bugbox M is the perfect way to improve isolation rates and is an easy to justify alternative to anaerobic
jars providing a more stable environment. Baker Ruskinn recognise that most general laboratories
inoculate samples with cultures on the bench. That's why samples are transferred, at up to 10 at a
time in the colour coded petri tubes - 15 seconds is all it takes to transfer and guarantee the best
isolation rates for your Microaerophilic organisms.
Add to this a range of optional accessories and you have the most convenient system for incubation
of microaerophilic available.
The Bugbox M is very affordable and maintenance costs are the lowest in the product class.
Features and Benefits
Compact size - Your personal workstation
Quick and easy direct access with the gloveless, cuffed Ezee Sleeve system.
Read plates easily without exposure to oxygen - energy-saving lighting provides perfect
illumination.
Petri dish holders for quick plate transfer included.
Can be built with ICO2N2IC to offer a microaerophilic environment in which to grow
facultative anaerobes.
Optimum cell environment
oAccurate temperature control - from ambient + 5˚C to 45˚C.
oAccurate and automated humidity control - no dry spots.
oPalladium catalyst maintains anaerobic environment - plus anaerobic colour-
indicator strips verify anaerobic conditions.
oEzee Sleeve system allows access without disrupting atmosphere within the
chamber.
Economic and reliable long-term savings
Standard dual gas operation - low gas consumption and running costs.
Lower cost per plate compared to anaerobic jars.
Minimal maintenance and downtime - with annual or biennial preventative maintenance kits
available.
The ICO2N2IC provides accurate control over O2(0.1% - 20.9% in 0.1% increments, can reach 23.0%
using separate 25% O2cylinder) and CO2(0.1% - 30% in 0.1% increments).

Bugbox M
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Table of Contents
Product Summary ...................................................................................................................................2
Features and Benefits ............................................................................................................................. 2
Overview ....................................................................................................................................6
Safety Instructions ........................................................................................................6
Regulatory compliance .................................................................................................7
Symbols......................................................................................................................... 7
Installation and relocation............................................................................................ 8
Weight and dimensions ................................................................................................9
Gas and electrical supply requirement....................................................................................10
Gas supply requirements ............................................................................................10
Gas regulator requirements........................................................................................10
Electrical supply requirements ...................................................................................10
Voltage and frequency requirements.........................................................................10
Power consumption....................................................................................................10
Powering the workstation .......................................................................................... 11
Workstation overview..............................................................................................................12
Workstation layout .....................................................................................................12
Component layout ......................................................................................................12
Front view .......................................................................................................12
Rear view ........................................................................................................13
Left side view –Standard humidifier.............................................................. 14
Right side view................................................................................................15
Control system layout.................................................................................................16
Control panel .................................................................................................. 16
Temperature controller ..................................................................................17
Port selector knob controller.......................................................................... 17
Foot switch control panel ...............................................................................18
Power entry panel .......................................................................................... 18
Temperature and humidity control .........................................................................................19
Temperature control...................................................................................................19
Setting the temperature;................................................................................ 19
Humidity control .........................................................................................................19
Standard humidity control.............................................................................. 19

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Environmental gas composition control.................................................................................. 19
Using the workstation..............................................................................................................20
Using the interlock......................................................................................................20
Interlock overview ..........................................................................................20
Opening the interlock outer door................................................................... 20
Opening the interlock inner door ...................................................................20
Transferring material into the workstation chamber via the interlock..........21
Removing material from the workstation chamber via the interlock ............21
Hand access to the main chamber.............................................................................. 22
Ezee Sleeve overview...................................................................................... 22
Entering the workstation chamber using the Ezee Sleeves............................23
Exiting the workstation chamber using the Ezee Sleeves............................... 24
Arm gas displacement volume and pressure changes ...................................24
Single Plate Entry System (SPES)................................................................................. 25
SPES overview.................................................................................................25
Using the SPES ................................................................................................26
Internal lights ..............................................................................................................27
Rear shelf ....................................................................................................................27
Internal power socket.................................................................................................27
Optional extras............................................................................................................27
Cable gland .....................................................................................................28
Universal cable gland......................................................................................28
Gas sample port..............................................................................................29
Vacuum port ................................................................................................... 29
Online video user guides.............................................................................................30
Cleaning and maintenance ......................................................................................................31
Cleaning the workstation............................................................................................31
Cleaning agents...............................................................................................31
Cleaning procedure –during and after each use............................................31
Cleaning procedure –deep clean ................................................................... 32
Maintaining the workstation –user maintenance ......................................................33
Filling the pressure relief/ humidifier tank..................................................... 33
Replacing an Ezee Sleeve................................................................................34
Replacing detox sachets .................................................................................35

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Installing/ replacing a catalyst sachet............................................................. 36
Replacing the mains plug fuse –UK users only ..............................................37
Replacing the mains fuses .............................................................................. 38
Service requirements.................................................................................................. 39
Spare parts and accessories........................................................................................ 39
Overview.........................................................................................................39
Spare parts and cleaning agents.....................................................................39
Accessories......................................................................................................40
Workstation malfunction............................................................................................41
Common problems and solutions............................................................................... 42
Workstation general problems....................................................................... 42
Interlock problems..........................................................................................43
Gas mixer and gas consumption problems.....................................................43
Warranty information.............................................................................................................. 45
Disposal information................................................................................................................ 46
Contact details ......................................................................................................................... 47

Bugbox M
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Overview
Please read this manual carefully to familiarise yourself with the operation and maintenance of your
Bugbox M workstation. Note: The workstation should be located in a well-ventilated area.
Safety Instructions
For your safety, the safety of others using the workstation and those around you;
The covers on both ends and the top of the workstation must not be removed by anyone
other than a qualified service engineer. There are no end user serviceable parts within these
covers.
The AC Mains outlet that the workstation is connected to, MUST not be obstructed by the
workstation or any other equipment, and MUST be accessible in case of emergency. In case
of emergency, disconnect the workstation from the AC Mains Outlet.
In case of damage to the workstation or ICO2N2IC disconnect the workstation / ICO2N2IC
from the AC Mains outlet and contact your Local Distributor for advice
The workstation must be connected to a protective earth.
Only the power cord supplied with the workstation should be used to connect the
workstation to the mains supply.
Only Ruskinn Technology Limited replacement parts should be used.
Gas regulators must be used for each gas supply. A 2 stage regulator should be used for a
bottled gas supply. The maximum supply pressure permissible is 4 bar gauge.
The maximum permissible concentration of Hydrogen in the anaerobic mixed gas is 5.5%
Hydrogen.
The mains supply voltage fluctuations must not exceed +/- 10% of the nominal mains
voltage.
Only the gasses specified in this manual may be used.
The maximum power rating of the internal socket must not be exceeded.
The humidifier screw cap must not be covered or blocked.
The humidifier/ pressure relief tank must not be overfilled.
The exhaust valve outlets must not be covered or blocked.
The spot light should not be used continuously for more than 10 minutes. The spot light
should be allowed to cool for at least 10 minutes between uses.
The cooling fan covers and vents must not be covered or blocked.
The workstation should not be lifted by the glove ports, the interlock or the Single Plate
Entry System.
The weight limit for the rear shelf is 5.5kg. The weight must be evenly distributed.
The use of Radioactive materials in the workstation is strictly prohibited.
If an uninterrupted power supply is used, both the Bugbox M and the ICO2N2IC must be
connected to the uninterrupted power supply.
FAILURE TO ADHERE TO THESE SAFETY INSTRUCTIONS COULD CAUSE SERIOUS INJURY AND WILL
INVALIDATE THE WORKSTATION WARRANTY. RUSKINN TECHNOLOGY LIMITED ACCEPTS NO
RESPONSIBILITY FOR ANY ACCIDENT, INJURY OR LOSS CAUSED BY UNSAFE OPERATION OF THE
WORKSTATION

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Regulatory compliance
This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage
Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive
204/108/EC.
Symbols
Before using the Bugbox M, please ensure that you are familiar with the symbols on the Bugbox M.
Figure 1 explains the symbols found on the Bugbox M;
Figure 1: Bugbox M symbols
Symbol
Meaning
Refer to user manual.
~
Alternating current
O
Off
I
On
Primary earth connection
This product complies with the essential EEA
requirements for Electrical Safety and
Electromagnetic compatibility as set out in
the EMC directive 2004/108/EC and the Low
Voltage Directive 2006/95/EC
Caution, do not remove covers. No end user
serviceable parts behind covers. Please refer
to this manual in all cases where this symbol
appears, in order to find out the nature of the
Potential Hazard and actions to be taken in
order to avoid the Hazard.

Bugbox M
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Symbol
Meaning
Warning, this equipment contains high
voltage circuitry.
Bugbox M contains hazardous components
and must not be disposed of at a household
waste site. Instead it should be taken to the
appropriate collection point for the recycling
of electrical and electronic equipment.
Date of manufacture in format
YYYY MM
Installation and relocation
Bugbox M should not be installed or relocated by anyone other than a qualified engineer. To arrange
installation or relocation, please contact your local distributor.

Bugbox M
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Weight and dimensions
The Bugbox M workstation weighs approximately 65kg. Figure 2 lists the dimensions of the Bugbox M
workstation;
Figure 2: Bugbox M workstation dimensions
External width
800 mm
External height
650 mm
External depth
660 mm
Workstation chamber internal width
500 mm
Workstation chamber internal height
420 mm
Workstation chamber internal depth
460 mm
Interlock internal width
100 mm
Interlock internal height
200 mm
Interlock internal depth
100 mm
Interlock outer door clear opening diameter
105 mm
Interlock inner door clear opening width
130 mm
Interlock inner door clear opening height
190 mm
NOTE: The interlock is specifically designed for use with Ruskinn Technology Limited small Petri dish
holders.

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Gas and electrical supply requirement
Gas supply requirements
The Bugbox M workstation requires:
Oxygen free nitrogen.
In addition to this, the Bugbox M workstation should be connected to ICO2N2IC (supplied with
the Bugbox M workstation). All other gas connections should be made to the ICO2N2IC.
See UM-011 ICO2N2IC user manual for more details.
The maximum permissible concentration of hydrogen in the anaerobic mixed gas is 5.5%. The use
of any other gas(ses) will invalidate the warranty and may cause serious injury. The Gas supplies
must be securely fastened after installation.
Gas regulator requirements
Gas regulators must be used with all gas supplies to the workstation. A 2 stage regulator must be used
for each bottled gas supply. Regulators should be available from your local gas supplier. Ruskinn
Technology Limited does not supply gas regulators.
The gas regulator should provide a minimum supply pressure of 3 Bar Gauge (42psi). The maximum
supply pressure permissible is 4 bar gauge. A supply pressure greater than this will damage internal
components of the workstation and will invalidate the warranty.
Electrical supply requirements
The workstation must be connected to a mains power supply. A power cord is supplied to connect the
workstation to the mains supply. Only the power cord supplied should be used to connect the
workstation to the mains supply. It is advised that the workstation be located no greater than 1 metre
from the plug socket. The plug socket should not be obstructed by the Bugbox M workstation. The
workstation must be connected to a protective earth.
Voltage and frequency requirements
To ensure safe operation of the workstation, it must be connected to a supply of the correct voltage
and frequency, as shown in the rating label (item 4 Figure 7). The mains supply voltage fluctuations
must not exceed +/- 10% of the nominal mains voltage.
Power consumption
Figure 3 shows the power consumption ratings;
Figure 3: Power consumption ratings
Supply voltage and frequency
Power consumption
240V AC, 50Hz
150 W
110V AC, 60Hz
200 W
Note: The power consumption is for normal operating conditions with no equipment connected to
the internal power supply. Power consumption will vary dependent upon the conditions inside the
workstation chamber and the conditions inside the room where the workstation is located.