Baker Bugbox M UM-020 User manual

UM-020
Bugbox M User Manual
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Bugbox M
UM-20 R4.0 Page 2of 47 Bugbox M User Manual
Product Summary
The world's smallest microaerophilic workstation packs all of the innovative features into a compact
bench print. Incorporating all of the familiar and proven features of the Bugbox, but tailored to
interface with the ICO2N2IC Advanced Gas Mixing System.
Like the Bugbox, taking just 800mm of bench space with a capacity to incubate up to 200 plates, the
Bugbox M is the perfect way to improve isolation rates and is an easy to justify alternative to anaerobic
jars providing a more stable environment. Baker Ruskinn recognise that most general laboratories
inoculate samples with cultures on the bench. That's why samples are transferred, at up to 10 at a
time in the colour coded petri tubes - 15 seconds is all it takes to transfer and guarantee the best
isolation rates for your Microaerophilic organisms.
Add to this a range of optional accessories and you have the most convenient system for incubation
of microaerophilic available.
The Bugbox M is very affordable and maintenance costs are the lowest in the product class.
Features and Benefits
Compact size - Your personal workstation
Quick and easy direct access with the gloveless, cuffed Ezee Sleeve system.
Read plates easily without exposure to oxygen - energy-saving lighting provides perfect
illumination.
Petri dish holders for quick plate transfer included.
Can be built with ICO2N2IC to offer a microaerophilic environment in which to grow
facultative anaerobes.
Optimum cell environment
oAccurate temperature control - from ambient + 5˚C to 45˚C.
oAccurate and automated humidity control - no dry spots.
oPalladium catalyst maintains anaerobic environment - plus anaerobic colour-
indicator strips verify anaerobic conditions.
oEzee Sleeve system allows access without disrupting atmosphere within the
chamber.
Economic and reliable long-term savings
Standard dual gas operation - low gas consumption and running costs.
Lower cost per plate compared to anaerobic jars.
Minimal maintenance and downtime - with annual or biennial preventative maintenance kits
available.
The ICO2N2IC provides accurate control over O2(0.1% - 20.9% in 0.1% increments, can reach 23.0%
using separate 25% O2cylinder) and CO2(0.1% - 30% in 0.1% increments).

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Table of Contents
Product Summary ...................................................................................................................................2
Features and Benefits ............................................................................................................................. 2
Overview ....................................................................................................................................6
Safety Instructions ........................................................................................................6
Regulatory compliance .................................................................................................7
Symbols......................................................................................................................... 7
Installation and relocation............................................................................................ 8
Weight and dimensions ................................................................................................9
Gas and electrical supply requirement....................................................................................10
Gas supply requirements ............................................................................................10
Gas regulator requirements........................................................................................10
Electrical supply requirements ...................................................................................10
Voltage and frequency requirements.........................................................................10
Power consumption....................................................................................................10
Powering the workstation .......................................................................................... 11
Workstation overview..............................................................................................................12
Workstation layout .....................................................................................................12
Component layout ......................................................................................................12
Front view .......................................................................................................12
Rear view ........................................................................................................13
Left side view –Standard humidifier.............................................................. 14
Right side view................................................................................................15
Control system layout.................................................................................................16
Control panel .................................................................................................. 16
Temperature controller ..................................................................................17
Port selector knob controller.......................................................................... 17
Foot switch control panel ...............................................................................18
Power entry panel .......................................................................................... 18
Temperature and humidity control .........................................................................................19
Temperature control...................................................................................................19
Setting the temperature;................................................................................ 19
Humidity control .........................................................................................................19
Standard humidity control.............................................................................. 19

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Environmental gas composition control.................................................................................. 19
Using the workstation..............................................................................................................20
Using the interlock......................................................................................................20
Interlock overview ..........................................................................................20
Opening the interlock outer door................................................................... 20
Opening the interlock inner door ...................................................................20
Transferring material into the workstation chamber via the interlock..........21
Removing material from the workstation chamber via the interlock ............21
Hand access to the main chamber.............................................................................. 22
Ezee Sleeve overview...................................................................................... 22
Entering the workstation chamber using the Ezee Sleeves............................23
Exiting the workstation chamber using the Ezee Sleeves............................... 24
Arm gas displacement volume and pressure changes ...................................24
Single Plate Entry System (SPES)................................................................................. 25
SPES overview.................................................................................................25
Using the SPES ................................................................................................26
Internal lights ..............................................................................................................27
Rear shelf ....................................................................................................................27
Internal power socket.................................................................................................27
Optional extras............................................................................................................27
Cable gland .....................................................................................................28
Universal cable gland......................................................................................28
Gas sample port..............................................................................................29
Vacuum port ................................................................................................... 29
Online video user guides.............................................................................................30
Cleaning and maintenance ......................................................................................................31
Cleaning the workstation............................................................................................31
Cleaning agents...............................................................................................31
Cleaning procedure –during and after each use............................................31
Cleaning procedure –deep clean ................................................................... 32
Maintaining the workstation –user maintenance ......................................................33
Filling the pressure relief/ humidifier tank..................................................... 33
Replacing an Ezee Sleeve................................................................................34
Replacing detox sachets .................................................................................35

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Installing/ replacing a catalyst sachet............................................................. 36
Replacing the mains plug fuse –UK users only ..............................................37
Replacing the mains fuses .............................................................................. 38
Service requirements.................................................................................................. 39
Spare parts and accessories........................................................................................ 39
Overview.........................................................................................................39
Spare parts and cleaning agents.....................................................................39
Accessories......................................................................................................40
Workstation malfunction............................................................................................41
Common problems and solutions............................................................................... 42
Workstation general problems....................................................................... 42
Interlock problems..........................................................................................43
Gas mixer and gas consumption problems.....................................................43
Warranty information.............................................................................................................. 45
Disposal information................................................................................................................ 46
Contact details ......................................................................................................................... 47

Bugbox M
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Overview
Please read this manual carefully to familiarise yourself with the operation and maintenance of your
Bugbox M workstation. Note: The workstation should be located in a well-ventilated area.
Safety Instructions
For your safety, the safety of others using the workstation and those around you;
The covers on both ends and the top of the workstation must not be removed by anyone
other than a qualified service engineer. There are no end user serviceable parts within these
covers.
The AC Mains outlet that the workstation is connected to, MUST not be obstructed by the
workstation or any other equipment, and MUST be accessible in case of emergency. In case
of emergency, disconnect the workstation from the AC Mains Outlet.
In case of damage to the workstation or ICO2N2IC disconnect the workstation / ICO2N2IC
from the AC Mains outlet and contact your Local Distributor for advice
The workstation must be connected to a protective earth.
Only the power cord supplied with the workstation should be used to connect the
workstation to the mains supply.
Only Ruskinn Technology Limited replacement parts should be used.
Gas regulators must be used for each gas supply. A 2 stage regulator should be used for a
bottled gas supply. The maximum supply pressure permissible is 4 bar gauge.
The maximum permissible concentration of Hydrogen in the anaerobic mixed gas is 5.5%
Hydrogen.
The mains supply voltage fluctuations must not exceed +/- 10% of the nominal mains
voltage.
Only the gasses specified in this manual may be used.
The maximum power rating of the internal socket must not be exceeded.
The humidifier screw cap must not be covered or blocked.
The humidifier/ pressure relief tank must not be overfilled.
The exhaust valve outlets must not be covered or blocked.
The spot light should not be used continuously for more than 10 minutes. The spot light
should be allowed to cool for at least 10 minutes between uses.
The cooling fan covers and vents must not be covered or blocked.
The workstation should not be lifted by the glove ports, the interlock or the Single Plate
Entry System.
The weight limit for the rear shelf is 5.5kg. The weight must be evenly distributed.
The use of Radioactive materials in the workstation is strictly prohibited.
If an uninterrupted power supply is used, both the Bugbox M and the ICO2N2IC must be
connected to the uninterrupted power supply.
FAILURE TO ADHERE TO THESE SAFETY INSTRUCTIONS COULD CAUSE SERIOUS INJURY AND WILL
INVALIDATE THE WORKSTATION WARRANTY. RUSKINN TECHNOLOGY LIMITED ACCEPTS NO
RESPONSIBILITY FOR ANY ACCIDENT, INJURY OR LOSS CAUSED BY UNSAFE OPERATION OF THE
WORKSTATION

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Regulatory compliance
This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage
Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive
204/108/EC.
Symbols
Before using the Bugbox M, please ensure that you are familiar with the symbols on the Bugbox M.
Figure 1 explains the symbols found on the Bugbox M;
Figure 1: Bugbox M symbols
Symbol
Meaning
Refer to user manual.
~
Alternating current
O
Off
I
On
Primary earth connection
This product complies with the essential EEA
requirements for Electrical Safety and
Electromagnetic compatibility as set out in
the EMC directive 2004/108/EC and the Low
Voltage Directive 2006/95/EC
Caution, do not remove covers. No end user
serviceable parts behind covers. Please refer
to this manual in all cases where this symbol
appears, in order to find out the nature of the
Potential Hazard and actions to be taken in
order to avoid the Hazard.

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Symbol
Meaning
Warning, this equipment contains high
voltage circuitry.
Bugbox M contains hazardous components
and must not be disposed of at a household
waste site. Instead it should be taken to the
appropriate collection point for the recycling
of electrical and electronic equipment.
Date of manufacture in format
YYYY MM
Installation and relocation
Bugbox M should not be installed or relocated by anyone other than a qualified engineer. To arrange
installation or relocation, please contact your local distributor.

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Weight and dimensions
The Bugbox M workstation weighs approximately 65kg. Figure 2 lists the dimensions of the Bugbox M
workstation;
Figure 2: Bugbox M workstation dimensions
External width
800 mm
External height
650 mm
External depth
660 mm
Workstation chamber internal width
500 mm
Workstation chamber internal height
420 mm
Workstation chamber internal depth
460 mm
Interlock internal width
100 mm
Interlock internal height
200 mm
Interlock internal depth
100 mm
Interlock outer door clear opening diameter
105 mm
Interlock inner door clear opening width
130 mm
Interlock inner door clear opening height
190 mm
NOTE: The interlock is specifically designed for use with Ruskinn Technology Limited small Petri dish
holders.

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Gas and electrical supply requirement
Gas supply requirements
The Bugbox M workstation requires:
Oxygen free nitrogen.
In addition to this, the Bugbox M workstation should be connected to ICO2N2IC (supplied with
the Bugbox M workstation). All other gas connections should be made to the ICO2N2IC.
See UM-011 ICO2N2IC user manual for more details.
The maximum permissible concentration of hydrogen in the anaerobic mixed gas is 5.5%. The use
of any other gas(ses) will invalidate the warranty and may cause serious injury. The Gas supplies
must be securely fastened after installation.
Gas regulator requirements
Gas regulators must be used with all gas supplies to the workstation. A 2 stage regulator must be used
for each bottled gas supply. Regulators should be available from your local gas supplier. Ruskinn
Technology Limited does not supply gas regulators.
The gas regulator should provide a minimum supply pressure of 3 Bar Gauge (42psi). The maximum
supply pressure permissible is 4 bar gauge. A supply pressure greater than this will damage internal
components of the workstation and will invalidate the warranty.
Electrical supply requirements
The workstation must be connected to a mains power supply. A power cord is supplied to connect the
workstation to the mains supply. Only the power cord supplied should be used to connect the
workstation to the mains supply. It is advised that the workstation be located no greater than 1 metre
from the plug socket. The plug socket should not be obstructed by the Bugbox M workstation. The
workstation must be connected to a protective earth.
Voltage and frequency requirements
To ensure safe operation of the workstation, it must be connected to a supply of the correct voltage
and frequency, as shown in the rating label (item 4 Figure 7). The mains supply voltage fluctuations
must not exceed +/- 10% of the nominal mains voltage.
Power consumption
Figure 3 shows the power consumption ratings;
Figure 3: Power consumption ratings
Supply voltage and frequency
Power consumption
240V AC, 50Hz
150 W
110V AC, 60Hz
200 W
Note: The power consumption is for normal operating conditions with no equipment connected to
the internal power supply. Power consumption will vary dependent upon the conditions inside the
workstation chamber and the conditions inside the room where the workstation is located.

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Powering the workstation
To switch the workstation on, press the power switch (item 2 in Figure 12).
To switch the workstation off, press the power switch (item 2 in Figure 12).
Note: A glove port should be left open when the workstation is switched off, as the pressure inside
the workstation will decrease as the workstation cools. The open glove port will allow the pressure
inside the workstation chamber to equalise with the external pressure.

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Workstation overview
Workstation layout
The Bugbox M workstation consists of 2 main areas; the workstation chamber and the interlock
chamber. The workstation chamber is the main working area of the workstation. Access to the
workstation chamber is provided by the glove ports and the interlock. The interlock consists of the
interlock chamber, with an inner and an outer door. The Bugbox M workstation is controlled via the
control panel.
Component layout
Please familiarise yourself with the layout of your Bugbox M workstation.
Front view
Figure 4 shows the front view of the Bugbox M workstation;
Figure 4: Bugbox M workstation front view
1. Glove ports (shown without Ezee Sleeves).
2. Port entry selector switch.
3. Interlock outer door.
4. Control panel.
5. Interlock outer door knob
2
4
5
3
1

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Rear view
Figure 5 shows the rear view of the Bugbox M workstation;
Figure 5: Bugbox M workstation rear view

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Left side view –Standard humidifier
Figure 6 shows the left side view of the Bugbox M workstation with the standard humidifier;
Figure 6: Bugbox M workstation left side view
1. Pressure relief tank cover (pressure relief tank bung).
2. Pressure relief tank.
3. Pressure relief tank cover.
4. Pressure relief tank overflow pipe.
5. Vacuum port (optional extra).
6. Exhaust valve
7. Condenser fan.
8. Single Plate Entry System.
Note: Please locate a suitable container below the tubing to collect any excess water from the
overflow tube.
1
2
4
5
7
8
6
3

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Right side view
Figure 7 shows the right side view of the Bugbox M workstation;
Figure 7: Bugbox M right side view
1. Interlock outer door knob.
2. Interlock outer door.
3. Power entry panel.
4. Serial number label.
5. PAT test label.
6. Mixed gas inlet.
7. Sample gas return.
8. Sample gas out.
1
2
4
5
8
7
6
3

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Control system layout
Please familiarise yourself with the control system layout of your Bugbox M workstation.
Control panel
Figure 8 shows the control panel of the Bugbox M workstation;
Figure 8: Bugbox M workstation control panel
1. Power indicator. Illuminates to indicate the workstation is connected to the mains and is
switched on.
2. Chamber light switch.
3. Gas Alarm switch. Press to switch the gas alarm on/ off.
4. Humidistat. Controls the level of humidity inside the workstation chamber.
5. Lock active indicator LED. Flashes to indicate that the interlock purge cycle is in progress.
6. Interlock purge button. Press to initiate the interlock purge cycle.
7. Gas demand indicator. Illuminates when there is a low pressure condition inside the
workstation chamber.
8. N2low indicator. Illuminates to indicate that the nitrogen supply has run out.
9. Temperature controller. See section 3.3.2, for more details.
1
2
4
5
7
8
6
3
9

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Temperature controller
Figure 9 shows the temperature controller;
Figure 9: Temperature controller
1. Not user accessible (For service personnel only)
2. Not user accessible (For service personnel only)
3. Number scrolling button. Use to move across digits. Use with buttons 4
& 5 to increase and decrease the temperature.
4. Decrease temperature (0.1°C increments) or humidity.
5. Increase temperature (0.1°C increments) or humidity.
For temperature control, the white value (PV- Process Value) is the actual temperature. The green
value (SV- Set Value) is the required value (the set point). All temperatures are in oC.
For ultrasonic humidity control, the white value is the actual relative humidity and the green value the
required relative humidity (the set point). All humidity’s are percentage relative humidity.
Port selector knob controller
Figure 10 shows the port selector knob controller;
Figure 10: Port selector knob controller
1
2
3
4
5

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Foot switch control panel
Figure 11 shows the foot switch controller which is used to operate the spot light, vacuum control
and interlock purge control.
Figure 11: Foot switch control panel
1. VAC for glove port vacuum control
2. LOCK for interlock purge control
3. SPOT for spotlight operation
Power entry panel
Figure 12 shows the power entry panel and label;
Figure 12: Power entry panel
1. Mains fuse holder.
2. On / off switch.
3. Mains lead connection.
4. Nitrogen gas inlet.
5. Primary earth stud.
6. ICO2N2IC umbilical connection.
1
2
3
1
2
3
4
5
6

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Temperature and humidity control
Temperature control
The Bugbox M workstation can control the workstation chamber temperature between ambient plus
5oC and 45oC.
The temperature in the main chamber can be set using the temperature controller on the control
panel.
Setting the temperature;
Referring to Figure 9:
To decrease the temperature set point, press button 4.
To increase the temperature set point, press button 5.
The temperature set point will increase or decrease in intervals of 0.1oC.
Humidity control
The Bugbox M workstation can control the workstation chamber humidity from ambient to 85%
relative humidity. There are 2 methods of humidity control on Bugbox M workstations;
Standard
Ultrasonic
Humidistat is the standard method of humidity control on the Bugbox M workstation. Ultrasonic is an
optional extra.
Note: Ultrasonic humidity can only be fitted at time of order; it cannot be added after manufacture of
the workstation.
Note: Any equipment installed in the workstation chamber must be compatible with the humidity
level inside the workstation chamber. If in doubt, consult the owner’s manual for any equipment that
is installed in the workstation chamber. Ruskinn Technology Limited accepts no responsibility for
damage to any equipment installed in the workstation chamber that is not suitable for the conditions
inside the workstation chamber.
Standard humidity control
To control the humidity of the workstation chamber using the humidistat (item 4 in Figure 8).
To increase the humidity, rotate the humidistat clockwise.
To decrease the humidity, rotate the humidistat anti-clockwise (counter-clockwise).
Note: For Bugbox M workstations fitted with standard humidity, a Petri dish/ tray of distilled water
should be placed in the workstation chamber to provide the humidity source for the workstation
chamber.
Environmental gas composition control
The environmental gas composition of the workstation chamber is controlled by the ICO2N2IC
Advanced Gas Mixer. For more information, please see UM-011 ICO2N2IC User Manual.

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Using the workstation
Using the interlock
The Bugbox M workstation has a specially designed interlock for transferring Petri dishes into and out
of the workstation chamber.
Interlock overview
The interlock consists of 2 main components;
Interlock outer door
Interlock inner door
The interlock is accessed by opening either the outer or inner door.
Note: Both doors should not be open at the same time.
Opening the interlock outer door
Before opening the interlock outer door, check that the interlock inner door is closed. To open the
interlock outer door, push the interlock outer door knob (item 5 in Figure 4) towards the workstation.
The interlock outer door will release. The interlock outer door can then be lifted open.
To close the interlock outer door, push the interlock outer door down. The door latch will click to
indicate that the door is closed.
Note: Do not open the interlock outer door if the interlock inner door is open. Do not place object(s)
on the interlock outer door, as this may damage the workstation.
Opening the interlock inner door
To open the interlock inner door;
Access the workstation chamber via the Ezee Sleeves. See section 6.2.2, for details.
Undo the inner door catch by turning the catch anti-clockwise (counter-clockwise).
Pull the inner door backwards to open the inner door.
To close the interlock inner door;
Push the interlock inner door closed.
Tighten the inner door catch by turning it clockwise. Do not over tighten the inner door catch.
Note: The interlock inner door should only be open when transferring materials from the interlock
chamber to the workstation chamber and vice versa. The interlock inner door should not be opened
if the interlock outer door is open.